Rroxscaffold_5G00378430

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004856
Physical Location & Seq
Reverse (-)
58909871 .. 58911922
2052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00378430.1

Sequence Viewer

Length: 1287 bp
ATGGAGGCCTATAGCTCTGTTCGGAGGGAGCATTTACTCCCACACTCCAGGCCAGAGTCCAAGAAAAAATCAACAATGGCACCACAGATATATGTGTGCGATTGTGCATTTAGCAGTATGCATGTCGAAATTATTTGTGACATGGTAGCTATTGCAAGGTTTTTGAATGTCACGCTTATAGTTCCCGAGTTGGATAAAACCTCCTTTTGGAATGATCGGAGTCAGTTTGAAGATATATTCGACGTGGATCACTTCATAGCGTCATTGAGCGATGAAGTCCGCATATTGAAAGTTCTGCCCCAGAAGCAGAGAAGAAGAATAGAAATGGCTTCCCTCTATTCCATGCCCCCTGCTAGTTGGTCTAATATGACATTTTACTACAACAGGGTTCTTCCTCAGATAAAGAAGTACGAAGTACTGCATTTCACTAAAACTGATACAAGACTTGCCAATAATGGGATCCCTGTGGAAGTTCAGAAAATGCGCTGCAAAGCAAATTATGAAGCCCTGAGGTTCACACCCCCAATTGAGGAATTGGGGAAGAAGATTGTAAGGATTCTGAGAGAAAGGGGCCCGTTCTTAGTTCTTCATCTCAGATATGAAATGGACATGTTAGCCTTTTCGGGTTGTACTGAAGGCTGCGATGACAAAGAAATTGAAGAATTGACAAATATGAGATACGCTTATCCATGGTGGAAAGAGAAAGTAATTAATTCCACAAAAAAAAGAAAATCTGGGAAATGCCCATTAACTCCCGAGGAGACTGCTCTTGCACTGAGGGCATTGGATATCGATCCTAGTATCCAAGTATACATAGCTGCAGGTGATATTTATGGGGGAGAAAGAAGAATGGCATCTCTGAGATTAGGCTTTCCACACATAGTTAAGAAAGAAACATTGCTGGAAGCCTCAAACCTTGAGCCCTTCCGAAACCATTCGAACCAAATGGCTGCATTGGATTACATTGTGTCACTGGAAAGTGATATCTTCGCTCCAACTTATGGGGGAAATATGGCAAGAGTTGTCGAAGGCCATAGACGGTACTTAGGATTCAGGACAACAATTCGTCTGGACAGAATGCTTCTAGTGAATTTGATAGACCAGTACAAGAAGGGTAGACTGAGCTGGGATGAATTTTCCCAGGCAGTGAAAACAGGACATGCCGATCGCATGGGTAGCCCTACACAAAGATTGGAGATTCCTGGTAAACCCAAGGAAGAGGAATACTTCTATAACAATCCACAGGAATGTTTACCACCAATTTCTACAAAACCAAAGAGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

428

Amino Acids

49.6

Weight (kDa)

8.9

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 44 - 349 1.7e-93 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 812
Acc36I ACCTGC 1 cut(s) 812
AccB1I GGYRCC 1 cut(s) 79
AccB7I CCANNNNNTGG 1 cut(s) 1001
AccI GTMKAC 2 cut(s) 810, 1117
AciI CCGC 1 cut(s) 280
AclWI GGATC 4 cut(s) 255, 454, 467, 788
AcsI RAATTY 2 cut(s) 1090, 1133
AcuI CTGAAG 1 cut(s) 654
AfaI GTAC 5 cut(s) 410, 417, 631, 1043, 1106
AfiI CCNNNNNNNGG 4 cut(s) 207, 456, 529, 1001
AflIII ACRYGT 1 cut(s) 609
AgsI TTSAA 4 cut(s) 166, 230, 289, 659
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 3 cut(s) 47, 1140, 1201
AluBI AGCT 4 cut(s) 15, 149, 818, 1125
AluI AGCT 4 cut(s) 15, 149, 818, 1125
Alw26I GTCTC 1 cut(s) 755
AlwI GGATC 4 cut(s) 255, 454, 467, 788
Ama87I CYCGRG 2 cut(s) 185, 755
AoxI GGCC 4 cut(s) 6, 50, 571, 1030
ApaI GGGCCC 1 cut(s) 575
ApeKI GCWGC 4 cut(s) 486, 639, 818, 950
ApoI RAATTY 2 cut(s) 1090, 1133
AseI ATTAAT 1 cut(s) 711
Asp700I GAANNNNTTC 1 cut(s) 934
AspLEI GCGC 1 cut(s) 486
AspS9I GGNCC 2 cut(s) 571, 572
AsuHPI GGTGA 1 cut(s) 836
AsuII TTCGAA 1 cut(s) 938
AvaI CYCGRG 2 cut(s) 185, 755
AxyI CCTNAGG 1 cut(s) 509
BaeGI GKGCMC 1 cut(s) 575
BamHI GGATCC 1 cut(s) 459
BanI GGYRCC 1 cut(s) 79
BanII GRGCYC 2 cut(s) 575, 924
BbvI GCAGC 4 cut(s) 473, 626, 805, 937
BcgI CGANNNNNNTGC 2 cut(s) 746, 780
BciT130I CCWGG 3 cut(s) 49, 1142, 1203
BciVI GTATCC 1 cut(s) 812
BcoDI GTCTC 1 cut(s) 755
BfaI CTAG 3 cut(s) 354, 798, 1085
BfmI CTRYAG 2 cut(s) 10, 819
BfuAI ACCTGC 1 cut(s) 812
BfuI GTATCC 1 cut(s) 812
BisI GCNGC 4 cut(s) 487, 640, 819, 951
BlsI GCNGC 4 cut(s) 488, 641, 820, 952
BmcAI AGTACT 1 cut(s) 417
Bme1390I CCNGG 3 cut(s) 49, 1142, 1203
BmeT110I CYCGRG 2 cut(s) 185, 755
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 571, 572
BmiI GGNNCC 4 cut(s) 81, 461, 572, 573
BmrFI CCNGG 3 cut(s) 49, 1142, 1203
BmsI GCATC 1 cut(s) 863
BpmI CTGGAG 1 cut(s) 31
Bpu14I TTCGAA 1 cut(s) 938
BpuEI CTTGAG 1 cut(s) 938
Bsa29I ATCGAT 1 cut(s) 792
BsaBI GATNNNNATC 1 cut(s) 792
BsaJI CCNNGG 4 cut(s) 689, 756, 1140, 1212
Bsc4I CCNNNNNNNGG 4 cut(s) 207, 456, 529, 1001
Bse1I ACTGG 2 cut(s) 978, 1102
Bse21I CCTNAGG 1 cut(s) 509
Bse3DI GCAATG 1 cut(s) 896
Bse8I GATNNNNATC 1 cut(s) 792
BseBI CCWGG 3 cut(s) 49, 1142, 1203
BseCI ATCGAT 1 cut(s) 792
BseDI CCNNGG 4 cut(s) 689, 756, 1140, 1212
BseGI GGATG 1 cut(s) 1135
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 4 cut(s) 207, 456, 529, 1001
BseMI GCAATG 1 cut(s) 896
BseMII CTCAG 7 cut(s) 410, 500, 551, 607, 767, 851, 1112
BseNI ACTGG 2 cut(s) 978, 1102
BseRI GAGGAG 1 cut(s) 773
BseSI GKGCMC 1 cut(s) 575
BseXI GCAGC 4 cut(s) 473, 626, 805, 937
BseYI CCCAGC 1 cut(s) 1125
Bsh1285I CGRYCG 1 cut(s) 1168
BshFI GGCC 4 cut(s) 8, 52, 573, 1032
BshNI GGYRCC 1 cut(s) 79
BshVI ATCGAT 1 cut(s) 792
BsiEI CGRYCG 1 cut(s) 1168
BsiHKCI CYCGRG 2 cut(s) 185, 755
BslI CCNNNNNNNGG 4 cut(s) 207, 456, 529, 1001
BsmAI GTCTC 1 cut(s) 755
BsmI GAATGC 1 cut(s) 1083
BsnI GGCC 4 cut(s) 8, 52, 573, 1032
BsoBI CYCGRG 2 cut(s) 185, 755
Bsp119I TTCGAA 1 cut(s) 938
Bsp120I GGGCCC 1 cut(s) 571
Bsp1286I GDGCHC 2 cut(s) 575, 924
Bsp143I GATC 5 cut(s) 214, 247, 459, 793, 1165
Bsp19I CCATGG 1 cut(s) 689
BspACI CCGC 1 cut(s) 280
BspANI GGCC 4 cut(s) 8, 52, 573, 1032
BspCNI CTCAG 7 cut(s) 409, 501, 552, 606, 768, 852, 1113
BspDI ATCGAT 1 cut(s) 792
BspLI GGNNCC 4 cut(s) 81, 461, 572, 573
BspMAI CTGCAG 1 cut(s) 823
BspMI ACCTGC 1 cut(s) 812
BspPI GGATC 4 cut(s) 255, 454, 467, 788
BspT104I TTCGAA 1 cut(s) 938
BspT107I GGYRCC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 896
BsrI ACTGG 2 cut(s) 978, 1102
BssECI CCNNGG 4 cut(s) 689, 756, 1140, 1212
BssMI GATC 5 cut(s) 214, 247, 459, 793, 1165
BssNAI GTATAC 1 cut(s) 811
BssT1I CCWWGG 2 cut(s) 689, 1212
Bst1107I GTATAC 1 cut(s) 811
Bst2UI CCWGG 3 cut(s) 49, 1142, 1203
Bst4CI ACNGT 1 cut(s) 1041
Bst6I CTCTTC 1 cut(s) 1212
BstBI TTCGAA 1 cut(s) 938
BstDEI CTNAG 9 cut(s) 396, 509, 560, 580, 593, 776, 860, 1045, 1121
BstDSI CCRYGG 1 cut(s) 689
BstF5I GGATG 1 cut(s) 1135
BstHHI GCGC 1 cut(s) 486
BstKTI GATC 5 cut(s) 217, 250, 462, 796, 1168
BstMAI GTCTC 1 cut(s) 755
BstMBI GATC 5 cut(s) 214, 247, 459, 793, 1165
BstMCI CGRYCG 1 cut(s) 1168
BstMWI GCNNNNNNNGC 3 cut(s) 304, 779, 1176
BstNI CCWGG 3 cut(s) 49, 1142, 1203
BstNSI RCATGY 3 cut(s) 125, 613, 1163
BstSCI CCNGG 3 cut(s) 47, 1140, 1201
BstSFI CTRYAG 2 cut(s) 10, 819
BstSLI GKGCMC 1 cut(s) 575
BstV1I GCAGC 4 cut(s) 473, 626, 805, 937
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BstZ17I GTATAC 1 cut(s) 811
Bsu15I ATCGAT 1 cut(s) 792
Bsu36I CCTNAGG 1 cut(s) 509
BsuI GTATCC 1 cut(s) 812
BsuRI GGCC 4 cut(s) 8, 52, 573, 1032
BsuTUI ATCGAT 1 cut(s) 792
BtgI CCRYGG 1 cut(s) 689
BtgZI GCGATG 2 cut(s) 285, 657
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 1 cut(s) 1135
BtsI GCAGTG 1 cut(s) 1152
BtsIMutI CAGTG 3 cut(s) 773, 971, 1152
BveI ACCTGC 1 cut(s) 812
CfoI GCGC 1 cut(s) 486
Cfr13I GGNCC 2 cut(s) 571, 572
ClaI ATCGAT 1 cut(s) 792
CseI GACGC 1 cut(s) 249
Csp6I GTAC 5 cut(s) 409, 416, 630, 1042, 1105
CviAII CATG 7 cut(s) 122, 142, 343, 610, 690, 1160, 1171
CviQI GTAC 5 cut(s) 409, 416, 630, 1042, 1105
DdeI CTNAG 9 cut(s) 396, 509, 560, 580, 593, 776, 860, 1045, 1121
DpnI GATC 5 cut(s) 216, 249, 461, 795, 1167
DpnII GATC 5 cut(s) 214, 247, 459, 793, 1165
Eam1104I CTCTTC 1 cut(s) 1212
EarI CTCTTC 1 cut(s) 1212
Eco130I CCWWGG 2 cut(s) 689, 1212
Eco147I AGGCCT 1 cut(s) 8
Eco24I GRGCYC 2 cut(s) 575, 924
Eco32I GATATC 2 cut(s) 790, 985
Eco57I CTGAAG 1 cut(s) 654
Eco81I CCTNAGG 1 cut(s) 509
Eco88I CYCGRG 2 cut(s) 185, 755
EcoO109I RGGNCCY 1 cut(s) 571
EcoRII CCWGG 3 cut(s) 47, 1140, 1201
EcoRV GATATC 2 cut(s) 790, 985
EcoT14I CCWWGG 2 cut(s) 689, 1212
EcoT22I ATGCAT 1 cut(s) 123
EcoT38I GRGCYC 2 cut(s) 575, 924
ErhI CCWWGG 2 cut(s) 689, 1212
FaeI CATG 7 cut(s) 125, 145, 346, 613, 693, 1163, 1174
FatI CATG 7 cut(s) 121, 141, 342, 609, 689, 1159, 1170
FblI GTMKAC 2 cut(s) 810, 1117
Fnu4HI GCNGC 4 cut(s) 487, 640, 819, 951
FokI GGATG 1 cut(s) 1142
FriOI GRGCYC 2 cut(s) 575, 924
Fsp4HI GCNGC 4 cut(s) 487, 640, 819, 951
FspBI CTAG 3 cut(s) 354, 798, 1085
GlaI GCGC 1 cut(s) 485
GluI GCNGC 4 cut(s) 487, 640, 819, 951
GsaI CCCAGC 1 cut(s) 1129
GsuI CTGGAG 1 cut(s) 31
HaeIII GGCC 4 cut(s) 8, 52, 573, 1032
HgaI GACGC 1 cut(s) 249
HhaI GCGC 1 cut(s) 486
Hin1II CATG 7 cut(s) 125, 145, 346, 613, 693, 1163, 1174
Hin6I GCGC 1 cut(s) 484
HinP1I GCGC 1 cut(s) 484
HinfI GANTC 5 cut(s) 56, 220, 556, 1050, 1198
HphI GGTGA 1 cut(s) 836
Hpy166II GTNNAC 5 cut(s) 516, 811, 1118, 1208, 1253
Hpy188I TCNGA 8 cut(s) 24, 219, 399, 477, 561, 596, 861, 929
Hpy188III TCNNGA 4 cut(s) 185, 755, 1054, 1070
Hpy8I GTNNAC 5 cut(s) 516, 811, 1118, 1208, 1253
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 4 cut(s) 629, 934, 1022, 1105
HpyCH4III ACNGT 1 cut(s) 1041
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 8 cut(s) 107, 121, 155, 421, 489, 773, 821, 953
HpyF10VI GCNNNNNNNGC 3 cut(s) 304, 779, 1176
HpyF3I CTNAG 9 cut(s) 396, 509, 560, 580, 593, 776, 860, 1045, 1121
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 7 cut(s) 125, 145, 346, 613, 693, 1163, 1174
HspAI GCGC 1 cut(s) 484
Kzo9I GATC 5 cut(s) 214, 247, 459, 793, 1165
LmnI GCTCC 2 cut(s) 28, 997
Lsp1109I GCAGC 4 cut(s) 473, 626, 805, 937
LweI GCATC 1 cut(s) 863
MaeI CTAG 3 cut(s) 354, 798, 1085
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 3 cut(s) 137, 169, 969
MalI GATC 5 cut(s) 216, 249, 461, 795, 1167
MboI GATC 5 cut(s) 214, 247, 459, 793, 1165
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 2 cut(s) 575, 924
MlyI GAGTC 2 cut(s) 65, 229
MmeI TCCRAC 2 cut(s) 171, 1019
Mph1103I ATGCAT 1 cut(s) 123
MroXI GAANNNNTTC 1 cut(s) 934
MseI TTAA 3 cut(s) 711, 749, 885
MslI CAYNNNNRTG 1 cut(s) 1246
MspR9I CCNGG 3 cut(s) 49, 1142, 1203
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 1083
MvaI CCWGG 3 cut(s) 49, 1142, 1203
MwoI GCNNNNNNNGC 3 cut(s) 304, 779, 1176
NcoI CCATGG 1 cut(s) 689
NdeII GATC 5 cut(s) 214, 247, 459, 793, 1165
NlaIII CATG 7 cut(s) 125, 145, 346, 613, 693, 1163, 1174
NlaIV GGNNCC 4 cut(s) 81, 461, 572, 573
NmuCI GTSAC 3 cut(s) 137, 169, 969
NsiI ATGCAT 1 cut(s) 123
NspI RCATGY 3 cut(s) 125, 613, 1163
NspV TTCGAA 1 cut(s) 938
PaqCI CACCTGC 1 cut(s) 812
PceI AGGCCT 1 cut(s) 8
PciI ACATGT 1 cut(s) 609
PctI GAATGC 1 cut(s) 1083
PdmI GAANNNNTTC 1 cut(s) 934
PfeI GAWTC 3 cut(s) 556, 1050, 1198
PflMI CCANNNNNTGG 1 cut(s) 1001
PkrI GCNGC 4 cut(s) 488, 641, 820, 952
Ple19I CGATCG 1 cut(s) 1168
PleI GAGTC 2 cut(s) 64, 228
PpsI GAGTC 2 cut(s) 64, 228
PscI ACATGT 1 cut(s) 609
PshBI ATTAAT 1 cut(s) 711
Psp6I CCWGG 3 cut(s) 47, 1140, 1201
PspFI CCCAGC 1 cut(s) 1125
PspGI CCWGG 3 cut(s) 47, 1140, 1201
PspN4I GGNNCC 4 cut(s) 81, 461, 572, 573
PspOMI GGGCCC 1 cut(s) 571
PspPI GGNCC 2 cut(s) 571, 572
PstI CTGCAG 1 cut(s) 823
PsuI RGATCY 1 cut(s) 459
PvuI CGATCG 1 cut(s) 1168
RsaI GTAC 5 cut(s) 410, 417, 631, 1043, 1106
RsaNI GTAC 5 cut(s) 409, 416, 630, 1042, 1105
RseI CAYNNNNRTG 1 cut(s) 1246
SaqAI TTAA 3 cut(s) 711, 749, 885
SatI GCNGC 4 cut(s) 487, 640, 819, 951
Sau3AI GATC 5 cut(s) 214, 247, 459, 793, 1165
Sau96I GGNCC 2 cut(s) 571, 572
ScaI AGTACT 1 cut(s) 417
SchI GAGTC 2 cut(s) 65, 229
ScrFI CCNGG 3 cut(s) 49, 1142, 1203
SduI GDGCHC 2 cut(s) 575, 924
SfaNI GCATC 1 cut(s) 863
SfcI CTRYAG 2 cut(s) 10, 819
SfuI TTCGAA 1 cut(s) 938
SmiMI CAYNNNNRTG 1 cut(s) 1246
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 1 cut(s) 280
SspMI CTAG 3 cut(s) 354, 798, 1085
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 47, 1140, 1201
StyI CCWWGG 2 cut(s) 689, 1212
TaaI ACNGT 1 cut(s) 1041
TaiI ACGT 1 cut(s) 246
TaqI TCGA 5 cut(s) 126, 240, 792, 938, 1026
TatI WGTACW 3 cut(s) 415, 629, 1104
TfiI GAWTC 3 cut(s) 556, 1050, 1198
Tru1I TTAA 3 cut(s) 711, 749, 885
Tru9I TTAA 3 cut(s) 711, 749, 885
TscAI CASTG 3 cut(s) 780, 978, 1152
TseFI GTSAC 3 cut(s) 137, 169, 969
TseI GCWGC 4 cut(s) 486, 639, 818, 950
Tsp45I GTSAC 3 cut(s) 137, 169, 969
TspDTI ATGAA 6 cut(s) 244, 288, 516, 578, 615, 1146
TspRI CASTG 3 cut(s) 780, 978, 1152
Van91I CCANNNNNTGG 1 cut(s) 1001
VspI ATTAAT 1 cut(s) 711
XapI RAATTY 2 cut(s) 1090, 1133
XceI RCATGY 3 cut(s) 125, 613, 1163
XmiI GTMKAC 2 cut(s) 810, 1117
XmnI GAANNNNTTC 1 cut(s) 934
XspI CTAG 3 cut(s) 354, 798, 1085
ZrmI AGTACT 1 cut(s) 417
Zsp2I ATGCAT 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.