Rw4G031070

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004856
Physical Location & Seq
Reverse (-)
57941247 .. 57944152
2906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031070.1

Sequence Viewer

Length: 1545 bp
ATGGATGGAAAAGATGATCTCGAATATGGCCAGGAGGTGAAGGATGAGAAGTCTTACATTAATGGGAAGCTCAAGTACTGGAAATATGCCGGAGGAATGAAGGCTGAACTCTTGAAGGATTTGATGAACGGGACTAATATCAAAGCCGAGAAGTACTTGAAAGGGTTGGTGATAGCTAGGATCCGACTCTCCCGGTTGAAATTGTGGGCGGTTCGACTGACGAAAGTGCTGCTACTCTTGGCTATTGCATTGCAGGTCAAGGGTTTGGGAGATACCTTGTTGCCAAAGATATCCAAAAGCTCTCTTCCCCCTGAAAGGATTTACGAAAACAATGGATATTTGATGGTTTCATCCAATGGAGGACTAAATCAGATGCGATCTGGCATATGTGACATGGTAGCAATTGCAAGATATTTAAATGTCACCCTTATAGTTCCTGAGTTGGATAACACCTCCTTCTGGAATGACCGCAGTCAATTTGAAGACATATTCGATGTGGATTATTTCATTACCTCATTGAGAGATGAGGTCAAAATTTTAAAAGAACTACCTCCTAAGCAGAAGAAGAAAGTGGAATTAGAATCCCTTTATTCCATGCCCCCAATTAGTTGGTCCAATATGACTTATTACTACAACACGATTCTTCCTCGCATACAAACATACGAAGTTGTGCACTTCACGAAAACAGATGCTAGGCTTGCCAACAATGGCATCCCTGAAGAGGTTCAGAAGCTTCGCTGCCGTGCGAATTACCAAGCTCTGAAATTCGCTCCTCCGATTGAGGCATTAGGCAAGAAAATTGTTAACATCCTCCGTGAAAGAGGCCCTTTCATGGTCCTTCATCTTCGGTACGAAATGGACATGGTGGCTTTTTCTGGTTGCACTGAGGGTTGCAATCGAGAAGAGATTGAAGAGACAACGAAAATGAGATATGCTTATCCATGGTGGAAAGAAAAGGAGATTGATTCAGAGAAGAAAAGGAAGGATGGACTTTGCCCTTTAACACCTGAAGAAACTGCTCTAGCATTAAGAGCACTAGGCATTGATCGTGATATTCAAGTCTATATTGCTGCTGGAGATATATACAAGGCAGAAAGGAGAATGGCACCTCTCAAAGAAGCTTTTCCAAATTTGGTGAGAAAGGAGACCTTGGTTGATGCATCAGATCTTCTCCCTCTCCAAAACCACTCGAACCAAATGGCGGCTCTGGACTACTACGTCTCGATAGAAAGTGACATATTTGTCCCAACATATGGAGGAAACATGGCAAAAGTAGTTGAAGGCCATAGAAGATACTTGGGATTCAAGAAAACCATTCTTCTAGACAGAAAGGCTCTGGTGGATCTGATAGACCAGTACAACAATGGAACCCTGAGCTGGAGCGAGTTCGCACTCGGAGTGAATACTGTTCATGCAGAGCGTATGGGACAACCAACTAGAAGAGTGGAGGTTCCAGGAAAACCTAAACTAGAGGACTACTTCTATACAAACCCAACTGAGTGTTTGTCACTGCCAGCTGAAGATGATGATCAACTATTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

514

Amino Acids

58.95

Weight (kDa)

7.52

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 113 - 433 2.1e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1217, 1241
Acc36I ACCTGC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 1105
AccB7I CCANNNNNTGG 1 cut(s) 1253
AciI CCGC 3 cut(s) 209, 469, 1202
AclWI GGATC 3 cut(s) 175, 188, 1350
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 3 cut(s) 534, 764, 1129
AcuI CTGAAG 3 cut(s) 738, 1029, 1539
AfaI GTAC 4 cut(s) 77, 155, 851, 1358
AfiI CCNNNNNNNGG 7 cut(s) 315, 459, 721, 832, 1201, 1253, 1377
AgsI TTSAA 8 cut(s) 115, 160, 199, 482, 911, 1058, 1280, 1306
AjnI CCWGG 2 cut(s) 30, 1453
AluBI AGCT 8 cut(s) 70, 176, 300, 733, 758, 1121, 1377, 1517
AluI AGCT 8 cut(s) 70, 176, 300, 733, 758, 1121, 1377, 1517
Alw21I GWGCWC 2 cut(s) 675, 1036
Alw26I GTCTC 3 cut(s) 908, 1139, 1225
Alw44I GTGCAC 1 cut(s) 671
AlwI GGATC 3 cut(s) 175, 188, 1350
AoxI GGCC 3 cut(s) 28, 823, 1282
ApaLI GTGCAC 1 cut(s) 671
ApeKI GCWGC 3 cut(s) 229, 738, 1070
ApoI RAATTY 3 cut(s) 534, 764, 1129
AseI ATTAAT 1 cut(s) 60
Asp700I GAANNNNTTC 2 cut(s) 723, 1122
AspS9I GGNCC 3 cut(s) 612, 824, 835
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 4 cut(s) 49, 181, 415, 1147
AvaII GGWCC 2 cut(s) 612, 835
BaeGI GKGCMC 1 cut(s) 675
BalI TGGCCA 1 cut(s) 30
BamHI GGATCC 1 cut(s) 180
BanI GGYRCC 1 cut(s) 1105
BarI GAAGNNNNNNTAC 2 cut(s) 59, 91
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 2 cut(s) 675, 1036
BbvI GCAGC 3 cut(s) 216, 725, 1057
BccI CCATC 2 cut(s) 337, 980
BceAI ACGGC 1 cut(s) 726
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 2 cut(s) 32, 1455
BclI TGATCA 1 cut(s) 1528
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 3 cut(s) 908, 1139, 1225
BfaI CTAG 7 cut(s) 177, 693, 1022, 1037, 1322, 1437, 1469
BfuAI ACCTGC 1 cut(s) 244
BglII AGATCT 1 cut(s) 1165
BisI GCNGC 4 cut(s) 230, 739, 1071, 1203
BlsI GCNGC 4 cut(s) 231, 740, 1072, 1204
BmcAI AGTACT 2 cut(s) 77, 155
Bme1390I CCNGG 3 cut(s) 32, 193, 1455
Bme18I GGWCC 2 cut(s) 612, 835
BmgT120I GGNCC 3 cut(s) 612, 824, 835
BmiI GGNNCC 4 cut(s) 182, 1107, 1369, 1452
BmrFI CCNGG 3 cut(s) 32, 193, 1455
BmsI GCATC 5 cut(s) 363, 679, 720, 1147, 1169
BoxI GACNNNNGTC 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 489
BpmI CTGGAG 2 cut(s) 1095, 1399
Bpu10I CCTNAGC 2 cut(s) 555, 1373
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 1 cut(s) 193
BsaBI GATNNNNATC 1 cut(s) 1527
BsaI GGTCTC 1 cut(s) 1139
BsaJI CCNNGG 2 cut(s) 941, 1149
Bsc4I CCNNNNNNNGG 7 cut(s) 315, 459, 721, 832, 1201, 1253, 1377
Bse1I ACTGG 2 cut(s) 83, 1354
Bse3DI GCAATG 1 cut(s) 248
Bse8I GATNNNNATC 1 cut(s) 1527
BseBI CCWGG 2 cut(s) 32, 1455
BseDI CCNNGG 2 cut(s) 941, 1149
BseGI GGATG 6 cut(s) 10, 49, 350, 711, 807, 991
BseJI GATNNNNATC 1 cut(s) 1527
BseLI CCNNNNNNNGG 7 cut(s) 315, 459, 721, 832, 1201, 1253, 1377
BseMI GCAATG 1 cut(s) 248
BseMII CTCAG 4 cut(s) 429, 876, 1364, 1488
BseNI ACTGG 2 cut(s) 83, 1354
BseRI GAGGAG 1 cut(s) 762
BseSI GKGCMC 1 cut(s) 675
BseXI GCAGC 3 cut(s) 216, 725, 1057
BshFI GGCC 3 cut(s) 30, 825, 1284
BshNI GGYRCC 1 cut(s) 1105
BsiHKAI GWGCWC 2 cut(s) 675, 1036
BsiSI CCGG 2 cut(s) 90, 193
BslFI GGGAC 3 cut(s) 145, 1229, 1440
BslI CCNNNNNNNGG 7 cut(s) 315, 459, 721, 832, 1201, 1253, 1377
BsmAI GTCTC 3 cut(s) 908, 1139, 1225
BsmBI CGTCTC 1 cut(s) 1225
BsmFI GGGAC 3 cut(s) 145, 1229, 1440
BsnI GGCC 3 cut(s) 30, 825, 1284
Bso31I GGTCTC 1 cut(s) 1139
Bsp1286I GDGCHC 2 cut(s) 675, 1036
Bsp143I GATC 7 cut(s) 16, 180, 377, 1045, 1165, 1342, 1528
Bsp19I CCATGG 1 cut(s) 941
BspACI CCGC 3 cut(s) 209, 469, 1202
BspANI GGCC 3 cut(s) 30, 825, 1284
BspCNI CTCAG 4 cut(s) 430, 877, 1365, 1489
BspLI GGNNCC 4 cut(s) 182, 1107, 1369, 1452
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 3 cut(s) 175, 188, 1350
BspT107I GGYRCC 1 cut(s) 1105
BspTNI GGTCTC 1 cut(s) 1139
BsrDI GCAATG 1 cut(s) 248
BsrI ACTGG 2 cut(s) 83, 1354
BssECI CCNNGG 2 cut(s) 941, 1149
BssMI GATC 7 cut(s) 16, 180, 377, 1045, 1165, 1342, 1528
BssT1I CCWWGG 2 cut(s) 941, 1149
Bst2UI CCWGG 2 cut(s) 32, 1455
Bst4CI ACNGT 2 cut(s) 1408, 1542
Bst6I CTCTTC 5 cut(s) 309, 714, 897, 906, 1435
BstC8I GCNNGC 2 cut(s) 699, 1515
BstDEI CTNAG 5 cut(s) 438, 555, 885, 1373, 1497
BstDSI CCRYGG 1 cut(s) 941
BstF5I GGATG 6 cut(s) 10, 49, 350, 711, 807, 991
BstKTI GATC 7 cut(s) 19, 183, 380, 1048, 1168, 1345, 1531
BstMAI GTCTC 3 cut(s) 908, 1139, 1225
BstMBI GATC 7 cut(s) 16, 180, 377, 1045, 1165, 1342, 1528
BstMWI GCNNNNNNNGC 2 cut(s) 698, 1031
BstNI CCWGG 2 cut(s) 32, 1455
BstPAI GACNNNNGTC 1 cut(s) 471
BstSCI CCNGG 3 cut(s) 30, 191, 1453
BstSLI GKGCMC 1 cut(s) 675
BstV1I GCAGC 3 cut(s) 216, 725, 1057
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 3 cut(s) 180, 1165, 1342
BstXI CCANNNNNNTGG 1 cut(s) 609
BstYI RGATCY 3 cut(s) 180, 1165, 1342
BsuRI GGCC 3 cut(s) 30, 825, 1284
BtgI CCRYGG 1 cut(s) 941
BtsCI GGATG 6 cut(s) 10, 49, 350, 711, 807, 991
BtsI GCAGTG 1 cut(s) 1508
BtsIMutI CAGTG 2 cut(s) 882, 1508
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 2 cut(s) 699, 1515
Cfr13I GGNCC 3 cut(s) 612, 824, 835
Csp6I GTAC 4 cut(s) 76, 154, 850, 1357
CviAII CATG 7 cut(s) 394, 595, 832, 862, 942, 1264, 1412
CviQI GTAC 4 cut(s) 76, 154, 850, 1357
DdeI CTNAG 5 cut(s) 438, 555, 885, 1373, 1497
DpnI GATC 7 cut(s) 18, 182, 379, 1047, 1167, 1344, 1530
DpnII GATC 7 cut(s) 16, 180, 377, 1045, 1165, 1342, 1528
DraI TTTAAA 2 cut(s) 417, 540
DrdI GACNNNNNNGTC 2 cut(s) 1217, 1241
DseDI GACNNNNNNGTC 2 cut(s) 1217, 1241
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 5 cut(s) 309, 714, 897, 906, 1435
EarI CTCTTC 5 cut(s) 309, 714, 897, 906, 1435
Eco130I CCWWGG 2 cut(s) 941, 1149
Eco31I GGTCTC 1 cut(s) 1139
Eco32I GATATC 1 cut(s) 291
Eco47I GGWCC 2 cut(s) 612, 835
Eco57I CTGAAG 3 cut(s) 738, 1029, 1539
EcoO109I RGGNCCY 1 cut(s) 824
EcoRII CCWGG 2 cut(s) 30, 1453
EcoRV GATATC 1 cut(s) 291
EcoT14I CCWWGG 2 cut(s) 941, 1149
EcoT22I ATGCAT 1 cut(s) 1162
ErhI CCWWGG 2 cut(s) 941, 1149
Esp3I CGTCTC 1 cut(s) 1225
FaeI CATG 7 cut(s) 397, 598, 835, 865, 945, 1267, 1415
FalI AAGNNNNNCTT 4 cut(s) 811, 843, 1133, 1165
FaqI GGGAC 3 cut(s) 145, 1229, 1440
FatI CATG 7 cut(s) 393, 594, 831, 861, 941, 1263, 1411
FauNDI CATATG 2 cut(s) 386, 1252
FbaI TGATCA 1 cut(s) 1528
Fnu4HI GCNGC 4 cut(s) 230, 739, 1071, 1203
FokI GGATG 6 cut(s) 17, 56, 337, 698, 794, 998
Fsp4HI GCNGC 4 cut(s) 230, 739, 1071, 1203
FspBI CTAG 7 cut(s) 177, 693, 1022, 1037, 1322, 1437, 1469
GluI GCNGC 4 cut(s) 230, 739, 1071, 1203
GsuI CTGGAG 2 cut(s) 1095, 1399
HaeIII GGCC 3 cut(s) 30, 825, 1284
HapII CCGG 2 cut(s) 90, 193
Hin1II CATG 7 cut(s) 397, 598, 835, 865, 945, 1267, 1415
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HindIII AAGCTT 2 cut(s) 731, 1119
HinfI GANTC 5 cut(s) 186, 581, 640, 965, 1302
HpaI GTTAAC 1 cut(s) 805
HpaII CCGG 2 cut(s) 90, 193
HphI GGTGA 4 cut(s) 49, 181, 415, 1147
Hpy166II GTNNAC 2 cut(s) 673, 805
Hpy188I TCNGA 9 cut(s) 185, 372, 729, 762, 777, 970, 1165, 1347, 1397
Hpy8I GTNNAC 2 cut(s) 673, 805
HpyAV CCTTC 7 cut(s) 34, 94, 109, 466, 848, 976, 1274
HpyCH4III ACNGT 2 cut(s) 1408, 1542
HpyCH4IV ACGT 1 cut(s) 1218
HpyCH4V TGCA 8 cut(s) 248, 253, 407, 673, 882, 894, 1160, 1415
HpyF10VI GCNNNNNNNGC 2 cut(s) 698, 1031
HpyF3I CTNAG 5 cut(s) 438, 555, 885, 1373, 1497
HpySE526I ACGT 1 cut(s) 1218
Hsp92II CATG 7 cut(s) 397, 598, 835, 865, 945, 1267, 1415
Ksp22I TGATCA 1 cut(s) 1528
KspAI GTTAAC 1 cut(s) 805
Kzo9I GATC 7 cut(s) 16, 180, 377, 1045, 1165, 1342, 1528
LmnI GCTCC 2 cut(s) 775, 1380
Lsp1109I GCAGC 3 cut(s) 216, 725, 1057
LweI GCATC 5 cut(s) 363, 679, 720, 1147, 1169
MaeI CTAG 7 cut(s) 177, 693, 1022, 1037, 1322, 1437, 1469
MaeII ACGT 1 cut(s) 1218
MaeIII GTNAC 4 cut(s) 389, 421, 1232, 1506
MalI GATC 7 cut(s) 18, 182, 379, 1047, 1167, 1344, 1530
MboI GATC 7 cut(s) 16, 180, 377, 1045, 1165, 1342, 1528
MfeI CAATTG 1 cut(s) 402
MflI RGATCY 3 cut(s) 180, 1165, 1342
MhlI GDGCHC 2 cut(s) 675, 1036
MlsI TGGCCA 1 cut(s) 30
MluNI TGGCCA 1 cut(s) 30
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 2 cut(s) 208, 423
Mox20I TGGCCA 1 cut(s) 30
Mph1103I ATGCAT 1 cut(s) 1162
MroXI GAANNNNTTC 2 cut(s) 723, 1122
MscI TGGCCA 1 cut(s) 30
MseI TTAA 6 cut(s) 60, 416, 539, 804, 1001, 1028
Msp20I TGGCCA 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 1517
MspI CCGG 2 cut(s) 90, 193
MspR9I CCNGG 3 cut(s) 32, 193, 1455
MunI CAATTG 1 cut(s) 402
MvaI CCWGG 2 cut(s) 32, 1455
MwoI GCNNNNNNNGC 2 cut(s) 698, 1031
NciI CCSGG 1 cut(s) 193
NcoI CCATGG 1 cut(s) 941
NdeI CATATG 2 cut(s) 386, 1252
NdeII GATC 7 cut(s) 16, 180, 377, 1045, 1165, 1342, 1528
NlaIII CATG 7 cut(s) 397, 598, 835, 865, 945, 1267, 1415
NlaIV GGNNCC 4 cut(s) 182, 1107, 1369, 1452
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 4 cut(s) 389, 421, 1232, 1506
NsiI ATGCAT 1 cut(s) 1162
PdmI GAANNNNTTC 2 cut(s) 723, 1122
PfeI GAWTC 4 cut(s) 581, 640, 965, 1302
PflMI CCANNNNNTGG 1 cut(s) 1253
PfoI TCCNGGA 1 cut(s) 1453
PkrI GCNGC 4 cut(s) 231, 740, 1072, 1204
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
PshAI GACNNNNGTC 1 cut(s) 471
PshBI ATTAAT 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 30, 1453
PspGI CCWGG 2 cut(s) 30, 1453
PspN4I GGNNCC 4 cut(s) 182, 1107, 1369, 1452
PspPI GGNCC 3 cut(s) 612, 824, 835
PsuI RGATCY 3 cut(s) 180, 1165, 1342
PvuII CAGCTG 1 cut(s) 1517
RsaI GTAC 4 cut(s) 77, 155, 851, 1358
RsaNI GTAC 4 cut(s) 76, 154, 850, 1357
SaqAI TTAA 6 cut(s) 60, 416, 539, 804, 1001, 1028
SatI GCNGC 4 cut(s) 230, 739, 1071, 1203
Sau3AI GATC 7 cut(s) 16, 180, 377, 1045, 1165, 1342, 1528
Sau96I GGNCC 3 cut(s) 612, 824, 835
ScaI AGTACT 2 cut(s) 77, 155
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 3 cut(s) 32, 193, 1455
SduI GDGCHC 2 cut(s) 675, 1036
SfaNI GCATC 5 cut(s) 363, 679, 720, 1147, 1169
SinI GGWCC 2 cut(s) 612, 835
SmiI ATTTAAAT 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SsiI CCGC 3 cut(s) 209, 469, 1202
SspMI CTAG 7 cut(s) 177, 693, 1022, 1037, 1322, 1437, 1469
StyD4I CCNGG 3 cut(s) 30, 191, 1453
StyI CCWWGG 2 cut(s) 941, 1149
SwaI ATTTAAAT 1 cut(s) 417
TaaI ACNGT 2 cut(s) 1408, 1542
TaiI ACGT 1 cut(s) 1221
TaqI TCGA 6 cut(s) 21, 214, 492, 898, 1190, 1223
TatI WGTACW 3 cut(s) 75, 153, 1356
TauI GCSGC 1 cut(s) 1205
TfiI GAWTC 4 cut(s) 581, 640, 965, 1302
Tru1I TTAA 6 cut(s) 60, 416, 539, 804, 1001, 1028
Tru9I TTAA 6 cut(s) 60, 416, 539, 804, 1001, 1028
TscAI CASTG 2 cut(s) 889, 1515
TseFI GTSAC 4 cut(s) 389, 421, 1232, 1506
TseI GCWGC 3 cut(s) 229, 738, 1070
Tsp45I GTSAC 4 cut(s) 389, 421, 1232, 1506
TspDTI ATGAA 7 cut(s) 113, 140, 339, 496, 820, 830, 1400
TspGWI ACGGA 1 cut(s) 803
TspRI CASTG 2 cut(s) 889, 1515
Van91I CCANNNNNTGG 1 cut(s) 1253
VneI GTGCAC 1 cut(s) 671
VpaK11BI GGWCC 2 cut(s) 612, 835
VspI ATTAAT 1 cut(s) 60
XapI RAATTY 3 cut(s) 534, 764, 1129
XbaI TCTAGA 1 cut(s) 1321
XcmI CCANNNNNNNNNTGG 1 cut(s) 1361
XmnI GAANNNNTTC 2 cut(s) 723, 1122
XspI CTAG 7 cut(s) 177, 693, 1022, 1037, 1322, 1437, 1469
ZrmI AGTACT 2 cut(s) 77, 155
Zsp2I ATGCAT 1 cut(s) 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.