Rorug04G0299400

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004856
Physical Location & Seq
Reverse (-)
47403350 .. 47406194
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0299400.1

Sequence Viewer

Length: 762 bp
ATGACGCTGGGAGATGCCACGACGACGACGACGACGCAGCAGCAAGCCTACGGCGAGCCATGGTACTGGGACAACCGCTACGCCAACGAATCCGGGGCGTTCGATTGGTACCAGAAGTACCAATCTCTAGCTCCCATTATCAATCTCTACGTCCCCCGCCACAACCACCTCCGCCACCGCATCCTCGTCGTCGGCTGCGGCAACTCAGCATTTAGCGAAGGAATGGCGGACGATGGGTATGAGGAGGTGGTTAGTATCGACATTTCATCTGTGGTCATCCAAGCTATGCAGGAGAAGCACTCCAATCGTCAAGACCTTAAATATTTGGAAATGGATGTGAGAGATATGAATGCTTTCCAAACCGCTTCCTTTGATGCTGTTGTTGACAAAGGAACTCTAGACTCTCTTCTGTGCGGAAGTAATTCACAACAAAATGCTGCTCGGATGCTCAGTGAAGTTTGGAGGGTCCTCAAGGATAAAGGAGTCTATATTCTGGTCACATATGGAGCCCCATTGTATCGTTTACGGTTGCTGAAAGAATCAAGCTCGTGGGTTATAAAACTCCATGTGATAGATAAACTTGTTTCCGATGATAAATCAGAACATCCAATATGGGAGCTGACAAATCCTGTTCCCTTGAATGATGATGGAAGCTCAGCAGAGGAATTGCTAGGAAACAACCCTGATGTCCATTTTATTTACATTTGTACGAAGGATAATTCTTCGAAGCCAGGCCTTAAGCGTGAAGTGTCAGTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

253

Amino Acids

28.61

Weight (kDa)

5.07

Isoelectric Point (pI)

27.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPMT PF05724 22 - 167 3.7e-07 Thiopurine S-methyltransferase (TPMT)
Methyltransf_31 PF13847 60 - 169 5.8e-15 Methyltransferase domain
Methyltransf_25 PF13649 61 - 161 1.8e-12 Methyltransferase domain
Methyltransf_11 PF08241 62 - 164 2.1e-15 Methyltransferase domain
Methyltransf_12 PF08242 62 - 162 6.3e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 557
Acc65I GGTACC 1 cut(s) 108
AccB1I GGYRCC 1 cut(s) 108
AccI GTMKAC 1 cut(s) 756
AciI CCGC 8 cut(s) 76, 157, 172, 178, 198, 227, 363, 414
AfaI GTAC 4 cut(s) 65, 110, 119, 709
AflII CTTAAG 1 cut(s) 737
AgsI TTSAA 1 cut(s) 640
AjnI CCWGG 1 cut(s) 730
AluBI AGCT 5 cut(s) 131, 284, 546, 619, 654
AluI AGCT 5 cut(s) 131, 284, 546, 619, 654
AoxI GGCC 1 cut(s) 733
ApeKI GCWGC 4 cut(s) 37, 40, 195, 437
Asp700I GAANNNNTTC 2 cut(s) 353, 421
Asp718I GGTACC 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 466
AsuC2I CCSGG 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 725
AvaII GGWCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 108
BanII GRGCYC 1 cut(s) 511
BauI CACGAG 1 cut(s) 547
BbvI GCAGC 4 cut(s) 49, 52, 182, 424
BccI CCATC 2 cut(s) 227, 641
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 4 cut(s) 169, 203, 287, 321
BciT130I CCWGG 1 cut(s) 732
BcnI CCSGG 1 cut(s) 94
BfaI CTAG 3 cut(s) 128, 398, 671
BfrI CTTAAG 1 cut(s) 737
BisI GCNGC 5 cut(s) 38, 41, 196, 199, 438
BlpI GCTNAGC 1 cut(s) 655
BlsI GCNGC 5 cut(s) 39, 42, 197, 200, 439
Bme1390I CCNGG 2 cut(s) 94, 732
Bme18I GGWCC 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 3 cut(s) 110, 467, 508
BmrFI CCNGG 2 cut(s) 94, 732
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 4 cut(s) 4, 189, 364, 435
BmuI ACTGGG 1 cut(s) 76
BplI GAGNNNNNCTC 2 cut(s) 284, 316
Bpu1102I GCTNAGC 1 cut(s) 655
Bpu14I TTCGAA 1 cut(s) 725
BpuEI CTTGAG 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 94
BsaJI CCNNGG 2 cut(s) 59, 93
Bse1I ACTGG 1 cut(s) 71
BseBI CCWGG 1 cut(s) 732
BseDI CCNNGG 2 cut(s) 59, 93
BseGI GGATG 5 cut(s) 180, 276, 340, 450, 604
BseMII CTCAG 3 cut(s) 219, 463, 669
BseNI ACTGG 1 cut(s) 71
BseRI GAGGAG 1 cut(s) 257
BseXI GCAGC 4 cut(s) 49, 52, 182, 424
BseYI CCCAGC 1 cut(s) 7
BshFI GGCC 1 cut(s) 735
BshNI GGYRCC 1 cut(s) 108
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 2 cut(s) 83, 137
BsmFI GGGAC 2 cut(s) 83, 137
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 1 cut(s) 735
Bsp119I TTCGAA 1 cut(s) 725
Bsp1286I GDGCHC 1 cut(s) 511
Bsp1720I GCTNAGC 1 cut(s) 655
Bsp19I CCATGG 1 cut(s) 59
BspACI CCGC 8 cut(s) 76, 157, 172, 178, 198, 227, 363, 414
BspANI GGCC 1 cut(s) 735
BspCNI CTCAG 3 cut(s) 218, 462, 668
BspLI GGNNCC 3 cut(s) 110, 467, 508
BspT104I TTCGAA 1 cut(s) 725
BspT107I GGYRCC 1 cut(s) 108
BspTI CTTAAG 1 cut(s) 737
BsrI ACTGG 1 cut(s) 71
BssECI CCNNGG 2 cut(s) 59, 93
BssSI CACGAG 1 cut(s) 547
BssT1I CCWWGG 1 cut(s) 59
Bst2BI CACGAG 1 cut(s) 547
Bst2UI CCWGG 1 cut(s) 732
Bst4CI ACNGT 1 cut(s) 528
Bst6I CTCTTC 1 cut(s) 411
BstAFI CTTAAG 1 cut(s) 737
BstBI TTCGAA 1 cut(s) 725
BstC8I GCNNGC 2 cut(s) 45, 56
BstDEI CTNAG 3 cut(s) 205, 449, 655
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 5 cut(s) 180, 276, 340, 450, 604
BstMWI GCNNNNNNNGC 1 cut(s) 295
BstNI CCWGG 1 cut(s) 732
BstSCI CCNGG 2 cut(s) 92, 730
BstV1I GCAGC 4 cut(s) 49, 52, 182, 424
BstXI CCANNNNNNTGG 1 cut(s) 66
BsuRI GGCC 1 cut(s) 735
BtgI CCRYGG 1 cut(s) 59
BtsCI GGATG 5 cut(s) 180, 276, 340, 450, 604
BtsIMutI CAGTG 1 cut(s) 457
Cac8I GCNNGC 2 cut(s) 45, 56
Cfr13I GGNCC 1 cut(s) 466
CseI GACGC 2 cut(s) 13, 43
Csp6I GTAC 4 cut(s) 64, 109, 118, 708
CviAII CATG 2 cut(s) 60, 566
CviQI GTAC 4 cut(s) 64, 109, 118, 708
DdeI CTNAG 3 cut(s) 205, 449, 655
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
EciI GGCGGA 2 cut(s) 161, 242
Eco130I CCWWGG 1 cut(s) 59
Eco147I AGGCCT 1 cut(s) 735
Eco24I GRGCYC 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 466
EcoO109I RGGNCCY 1 cut(s) 466
EcoRII CCWGG 1 cut(s) 730
EcoT14I CCWWGG 1 cut(s) 59
EcoT38I GRGCYC 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 59
FaeI CATG 2 cut(s) 63, 569
FaqI GGGAC 2 cut(s) 83, 137
FatI CATG 2 cut(s) 59, 565
FauI CCCGC 1 cut(s) 164
FauNDI CATATG 1 cut(s) 502
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 5 cut(s) 38, 41, 196, 199, 438
FokI GGATG 5 cut(s) 167, 263, 347, 457, 591
FriOI GRGCYC 1 cut(s) 511
Fsp4HI GCNGC 5 cut(s) 38, 41, 196, 199, 438
FspBI CTAG 3 cut(s) 128, 398, 671
GluI GCNGC 5 cut(s) 38, 41, 196, 199, 438
GsaI CCCAGC 1 cut(s) 11
HaeIII GGCC 1 cut(s) 735
HapII CCGG 1 cut(s) 93
HgaI GACGC 2 cut(s) 13, 43
Hin1II CATG 2 cut(s) 63, 569
HincII GTYRAC 2 cut(s) 385, 757
HindII GTYRAC 2 cut(s) 385, 757
HinfI GANTC 4 cut(s) 89, 401, 483, 539
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 3 cut(s) 385, 524, 757
Hpy188I TCNGA 3 cut(s) 444, 589, 601
Hpy188III TCNNGA 2 cut(s) 311, 398
Hpy8I GTNNAC 3 cut(s) 385, 524, 757
Hpy99I CGWCG 7 cut(s) 25, 28, 31, 34, 37, 191, 194
HpyAV CCTTC 2 cut(s) 212, 706
HpyCH4III ACNGT 1 cut(s) 528
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 1 cut(s) 289
HpyF10VI GCNNNNNNNGC 1 cut(s) 295
HpyF3I CTNAG 3 cut(s) 205, 449, 655
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 2 cut(s) 63, 569
KpnI GGTACC 1 cut(s) 112
LmnI GCTCC 3 cut(s) 136, 506, 616
LpnPI CCDG 9 cut(s) 52, 106, 125, 275, 479, 642, 696, 717, 744
Lsp1109I GCAGC 4 cut(s) 49, 52, 182, 424
LweI GCATC 4 cut(s) 4, 189, 364, 435
MaeI CTAG 3 cut(s) 128, 398, 671
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 496
MboII GAAGA 2 cut(s) 398, 714
MhlI GDGCHC 1 cut(s) 511
MluCI AATT 3 cut(s) 421, 665, 718
MlyI GAGTC 2 cut(s) 395, 492
MnlI CCTC 7 cut(s) 179, 194, 235, 238, 456, 479, 655
MroXI GAANNNNTTC 2 cut(s) 353, 421
MseI TTAA 2 cut(s) 318, 738
MspCI CTTAAG 1 cut(s) 737
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 2 cut(s) 94, 732
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 1 cut(s) 732
MwoI GCNNNNNNNGC 1 cut(s) 295
NciI CCSGG 1 cut(s) 94
NcoI CCATGG 1 cut(s) 59
NdeI CATATG 1 cut(s) 502
NlaIII CATG 2 cut(s) 63, 569
NlaIV GGNNCC 3 cut(s) 110, 467, 508
NmuCI GTSAC 1 cut(s) 496
NspV TTCGAA 1 cut(s) 725
PceI AGGCCT 1 cut(s) 735
PcsI WCGNNNNNNNCGW 2 cut(s) 26, 29
PctI GAATGC 1 cut(s) 355
PdmI GAANNNNTTC 2 cut(s) 353, 421
PfeI GAWTC 2 cut(s) 89, 539
PkrI GCNGC 5 cut(s) 39, 42, 197, 200, 439
PleI GAGTC 2 cut(s) 395, 491
PpsI GAGTC 2 cut(s) 395, 491
PpuMI RGGWCCY 1 cut(s) 466
PsiI TTATAA 1 cut(s) 557
Psp5II RGGWCCY 1 cut(s) 466
Psp6I CCWGG 1 cut(s) 730
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 730
PspN4I GGNNCC 3 cut(s) 110, 467, 508
PspPI GGNCC 1 cut(s) 466
PspPPI RGGWCCY 1 cut(s) 466
RsaI GTAC 4 cut(s) 65, 110, 119, 709
RsaNI GTAC 4 cut(s) 64, 109, 118, 708
SalI GTCGAC 1 cut(s) 755
SaqAI TTAA 2 cut(s) 318, 738
SatI GCNGC 5 cut(s) 38, 41, 196, 199, 438
Sau96I GGNCC 1 cut(s) 466
SchI GAGTC 2 cut(s) 395, 492
ScrFI CCNGG 2 cut(s) 94, 732
SduI GDGCHC 1 cut(s) 511
SetI ASST 9 cut(s) 133, 153, 171, 249, 286, 318, 548, 621, 656
SfaNI GCATC 4 cut(s) 4, 189, 364, 435
SfuI TTCGAA 1 cut(s) 725
SinI GGWCC 1 cut(s) 466
SmlI CTYRAG 2 cut(s) 470, 737
SmoI CTYRAG 2 cut(s) 470, 737
Sse9I AATT 3 cut(s) 421, 665, 718
SseBI AGGCCT 1 cut(s) 735
SsiI CCGC 8 cut(s) 76, 157, 172, 178, 198, 227, 363, 414
SspI AATATT 1 cut(s) 323
SspMI CTAG 3 cut(s) 128, 398, 671
StuI AGGCCT 1 cut(s) 735
StyD4I CCNGG 2 cut(s) 92, 730
StyI CCWWGG 1 cut(s) 59
TaaI ACNGT 1 cut(s) 528
TaiI ACGT 1 cut(s) 153
TaqI TCGA 4 cut(s) 102, 258, 725, 756
TasI AATT 3 cut(s) 421, 665, 718
TauI GCSGC 1 cut(s) 201
TfiI GAWTC 2 cut(s) 89, 539
Tru1I TTAA 2 cut(s) 318, 738
Tru9I TTAA 2 cut(s) 318, 738
TscAI CASTG 1 cut(s) 457
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 4 cut(s) 37, 40, 195, 437
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 2 cut(s) 255, 362
TspRI CASTG 1 cut(s) 457
Vha464I CTTAAG 1 cut(s) 737
VpaK11BI GGWCC 1 cut(s) 466
XbaI TCTAGA 1 cut(s) 397
XmiI GTMKAC 1 cut(s) 756
XmnI GAANNNNTTC 2 cut(s) 353, 421
XspI CTAG 3 cut(s) 128, 398, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.