Rh4AG354300

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004856
Physical Location & Seq
Reverse (-)
66309559 .. 66312637
3079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG354300.1

Sequence Viewer

Length: 1560 bp
ATGGATGGAAAAGATGATCTCAAGGACCCGGAATATGGCCAGGAGGTGAAGGATGAGAAGTCTTACTCTTACATTAATGGGAAGCTCAAGTACTGGAAATATGCCGGAGGAATGAAGGCTGAACTCTTGAAGGATTTGATGAACGGGACTAATATCAAAGCCGAGAAGTACTTGAAAGGGTTGGTGATAGCTAGGACCCGACTCGCCCGGTTGAAATTGTGGGCGGTTCGACTGACGAAAGTGCTGCTACTCTTGGCTATTGCATTGCAGGTCAAGGGTTTGGGAGATACCTTGTTGCCAAAGATATCCAAAAGCTCTCTTCCCCCTGAAAGGATTTACGAAAACAATGGATATTTGATGGTTTCATCCAATGGAGGACTAAATCAGATGCGATCTGGCATATGTGACATGGTAGCAATTGCAAGATATTTAAATGTCACCCTTATAGTTCCTGAGTTGGATAACACCTCCTTCTGGAATGACCGCAGTCAATTTGAAGACATATTCGATGTGGATTATTTCATTACCTCATTGAGAGATGAGGTCAAAATTTTAAAAGAACTACCTCCTAAGCAGAAGAAGAAAGTGGAATTAGAATCCCTTTATTCCATGCCCCCAATTAGTTGGTCCAATATGACTTATTACTATAACACGATTCTTCCTCGCATACAAACATACGAAGTTGTGCACTTCACGAAAACAGATGCTAGGCTTGCCAACAATGGCATCCCTGAAGAGGTTCAGAAGCTTCGCTGCCGTGCTAATTACCAAGCTCTGAAATTCGCTCCTCCGATTGAGGCATTAGGCAAGAAAATTGTTAACATCCTCCGTGAAAGAGGCCCTTTCATGGTCCTTCATCTTCGGTACGAAATGGACATGGTGGCTTTTTCTGGTTGCACTGAGGGTTGCAATCGAGAAGAGATTGAAGAGACAACGAAAATGAGATATGCTTATCCATGGTGGAAAGAAAAGGAGATTGATTCAGAGAAGAAAAGGAAGGATGGACTTTGCCCTTTAACACCTGAAGAAACTGCTCTAGCATTAAGAGCACTAGGCATTGATCGTGATATTCAAGTCTATATTGCTGCTGGAGATATATACAAGGCAGAAAGGAGAATGGCACCTCTCAAAGAAGCTTTTCCAAATTTGGTGAGAAAGGAGACGTTGGTTGATGCATCAGATCTTCTCCCTCTCCAAAACCACTCGAACCAAATGGCGGCTCTGGACTACTACGTCTCGATAGAAAGTGACATCTTTGTCCCAACATATGGAGGAAACATGGCAAAAGTAGTTGAAGGCCATAGAAGATACTTGGGATTCAAGAAAACCATTCTTCTAGACAGAAAGGCTCTGGTGGATCTGATAGACCAGTACAACAATGGAACCCTGAGCTGGAGCGAGTTCGCACTCGGAGTGAATACTGTTCATGCAGAGCGTATGGGACAACCAACTAGAAGAGTGGAGGTTCCAGGAAAACCTAAACTAGAGGACTACTTCTATACAAACCCAACTGAGTGTTTGTCACTGCCAGCTGAAGATGATGATCAACTATTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

519

Amino Acids

59.51

Weight (kDa)

7.51

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 118 - 438 2.1e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1232, 1256
Acc36I ACCTGC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 1120
AccB7I CCANNNNNTGG 1 cut(s) 1268
AciI CCGC 3 cut(s) 224, 484, 1217
AclWI GGATC 1 cut(s) 1365
AcoI YGGCCR 1 cut(s) 37
AcsI RAATTY 3 cut(s) 549, 779, 1144
AcuI CTGAAG 3 cut(s) 753, 1044, 1554
AfaI GTAC 4 cut(s) 92, 170, 866, 1373
AfiI CCNNNNNNNGG 8 cut(s) 35, 330, 474, 736, 847, 1216, 1268, 1392
AgsI TTSAA 8 cut(s) 130, 175, 214, 497, 926, 1073, 1295, 1321
AjnI CCWGG 2 cut(s) 39, 1468
AluBI AGCT 8 cut(s) 85, 191, 315, 748, 773, 1136, 1392, 1532
AluI AGCT 8 cut(s) 85, 191, 315, 748, 773, 1136, 1392, 1532
Alw21I GWGCWC 2 cut(s) 690, 1051
Alw26I GTCTC 3 cut(s) 923, 1154, 1240
Alw44I GTGCAC 1 cut(s) 686
AlwI GGATC 1 cut(s) 1365
AoxI GGCC 3 cut(s) 37, 838, 1297
ApaLI GTGCAC 1 cut(s) 686
ApeKI GCWGC 3 cut(s) 244, 753, 1085
ApoI RAATTY 3 cut(s) 549, 779, 1144
AseI ATTAAT 1 cut(s) 75
Asp700I GAANNNNTTC 2 cut(s) 738, 1137
AspS9I GGNCC 5 cut(s) 25, 195, 627, 839, 850
AsuC2I CCSGG 2 cut(s) 29, 208
AsuHPI GGTGA 4 cut(s) 58, 196, 430, 1162
AvaII GGWCC 4 cut(s) 25, 195, 627, 850
BaeGI GKGCMC 1 cut(s) 690
BalI TGGCCA 1 cut(s) 39
BanI GGYRCC 1 cut(s) 1120
BarI GAAGNNNNNNTAC 2 cut(s) 74, 106
BbsI GAAGAC 1 cut(s) 504
Bbv12I GWGCWC 2 cut(s) 690, 1051
BbvI GCAGC 3 cut(s) 231, 740, 1072
BccI CCATC 2 cut(s) 352, 995
BceAI ACGGC 1 cut(s) 741
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 2 cut(s) 41, 1470
BclI TGATCA 1 cut(s) 1543
BcnI CCSGG 2 cut(s) 29, 208
BcoDI GTCTC 3 cut(s) 923, 1154, 1240
BfaI CTAG 7 cut(s) 192, 708, 1037, 1052, 1337, 1452, 1484
BfuAI ACCTGC 1 cut(s) 259
BglII AGATCT 1 cut(s) 1180
BisI GCNGC 4 cut(s) 245, 754, 1086, 1218
BlsI GCNGC 4 cut(s) 246, 755, 1087, 1219
BmcAI AGTACT 2 cut(s) 92, 170
Bme1390I CCNGG 4 cut(s) 29, 41, 208, 1470
Bme18I GGWCC 4 cut(s) 25, 195, 627, 850
BmgT120I GGNCC 5 cut(s) 25, 195, 627, 839, 850
BmiI GGNNCC 5 cut(s) 27, 197, 1122, 1384, 1467
BmrFI CCNGG 4 cut(s) 29, 41, 208, 1470
BmsI GCATC 5 cut(s) 378, 694, 735, 1162, 1184
BoxI GACNNNNGTC 1 cut(s) 486
BpiI GAAGAC 1 cut(s) 504
BpmI CTGGAG 2 cut(s) 1110, 1414
Bpu10I CCTNAGC 2 cut(s) 570, 1388
BpuEI CTTGAG 2 cut(s) 5, 71
BpuMI CCSGG 2 cut(s) 29, 208
BsaBI GATNNNNATC 1 cut(s) 1542
BsaJI CCNNGG 1 cut(s) 956
Bsc4I CCNNNNNNNGG 8 cut(s) 35, 330, 474, 736, 847, 1216, 1268, 1392
Bse1I ACTGG 2 cut(s) 98, 1369
Bse3DI GCAATG 1 cut(s) 263
Bse8I GATNNNNATC 1 cut(s) 1542
BseBI CCWGG 2 cut(s) 41, 1470
BseDI CCNNGG 1 cut(s) 956
BseGI GGATG 6 cut(s) 10, 58, 365, 726, 822, 1006
BseJI GATNNNNATC 1 cut(s) 1542
BseLI CCNNNNNNNGG 8 cut(s) 35, 330, 474, 736, 847, 1216, 1268, 1392
BseMI GCAATG 1 cut(s) 263
BseMII CTCAG 4 cut(s) 444, 891, 1379, 1503
BseNI ACTGG 2 cut(s) 98, 1369
BseRI GAGGAG 1 cut(s) 777
BseSI GKGCMC 1 cut(s) 690
BseXI GCAGC 3 cut(s) 231, 740, 1072
BshFI GGCC 3 cut(s) 39, 840, 1299
BshNI GGYRCC 1 cut(s) 1120
BsiHKAI GWGCWC 2 cut(s) 690, 1051
BsiSI CCGG 3 cut(s) 29, 105, 208
BslFI GGGAC 3 cut(s) 160, 1244, 1455
BslI CCNNNNNNNGG 8 cut(s) 35, 330, 474, 736, 847, 1216, 1268, 1392
BsmAI GTCTC 3 cut(s) 923, 1154, 1240
BsmBI CGTCTC 2 cut(s) 1154, 1240
BsmFI GGGAC 3 cut(s) 160, 1244, 1455
BsnI GGCC 3 cut(s) 39, 840, 1299
Bsp1286I GDGCHC 2 cut(s) 690, 1051
Bsp143I GATC 6 cut(s) 16, 392, 1060, 1180, 1357, 1543
Bsp19I CCATGG 1 cut(s) 956
BspACI CCGC 3 cut(s) 224, 484, 1217
BspANI GGCC 3 cut(s) 39, 840, 1299
BspCNI CTCAG 4 cut(s) 445, 892, 1380, 1504
BspLI GGNNCC 5 cut(s) 27, 197, 1122, 1384, 1467
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 1 cut(s) 1365
BspT107I GGYRCC 1 cut(s) 1120
BsrDI GCAATG 1 cut(s) 263
BsrI ACTGG 2 cut(s) 98, 1369
BssECI CCNNGG 1 cut(s) 956
BssMI GATC 6 cut(s) 16, 392, 1060, 1180, 1357, 1543
BssT1I CCWWGG 1 cut(s) 956
Bst2UI CCWGG 2 cut(s) 41, 1470
Bst4CI ACNGT 2 cut(s) 1423, 1557
Bst6I CTCTTC 5 cut(s) 324, 729, 912, 921, 1450
BstC8I GCNNGC 2 cut(s) 714, 1530
BstDEI CTNAG 5 cut(s) 453, 570, 900, 1388, 1512
BstDSI CCRYGG 1 cut(s) 956
BstF5I GGATG 6 cut(s) 10, 58, 365, 726, 822, 1006
BstKTI GATC 6 cut(s) 19, 395, 1063, 1183, 1360, 1546
BstMAI GTCTC 3 cut(s) 923, 1154, 1240
BstMBI GATC 6 cut(s) 16, 392, 1060, 1180, 1357, 1543
BstMWI GCNNNNNNNGC 2 cut(s) 713, 1046
BstNI CCWGG 2 cut(s) 41, 1470
BstPAI GACNNNNGTC 1 cut(s) 486
BstSCI CCNGG 4 cut(s) 27, 39, 206, 1468
BstSLI GKGCMC 1 cut(s) 690
BstV1I GCAGC 3 cut(s) 231, 740, 1072
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 2 cut(s) 1180, 1357
BstXI CCANNNNNNTGG 1 cut(s) 624
BstYI RGATCY 2 cut(s) 1180, 1357
BsuRI GGCC 3 cut(s) 39, 840, 1299
BtgI CCRYGG 1 cut(s) 956
BtsCI GGATG 6 cut(s) 10, 58, 365, 726, 822, 1006
BtsI GCAGTG 1 cut(s) 1523
BtsIMutI CAGTG 2 cut(s) 897, 1523
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 2 cut(s) 714, 1530
Cfr13I GGNCC 5 cut(s) 25, 195, 627, 839, 850
Csp6I GTAC 4 cut(s) 91, 169, 865, 1372
CviAII CATG 7 cut(s) 409, 610, 847, 877, 957, 1279, 1427
CviQI GTAC 4 cut(s) 91, 169, 865, 1372
DdeI CTNAG 5 cut(s) 453, 570, 900, 1388, 1512
DpnI GATC 6 cut(s) 18, 394, 1062, 1182, 1359, 1545
DpnII GATC 6 cut(s) 16, 392, 1060, 1180, 1357, 1543
DraI TTTAAA 2 cut(s) 432, 555
DrdI GACNNNNNNGTC 2 cut(s) 1232, 1256
DseDI GACNNNNNNGTC 2 cut(s) 1232, 1256
EaeI YGGCCR 1 cut(s) 37
Eam1104I CTCTTC 5 cut(s) 324, 729, 912, 921, 1450
EarI CTCTTC 5 cut(s) 324, 729, 912, 921, 1450
Eco130I CCWWGG 1 cut(s) 956
Eco32I GATATC 1 cut(s) 306
Eco47I GGWCC 4 cut(s) 25, 195, 627, 850
Eco57I CTGAAG 3 cut(s) 753, 1044, 1554
EcoO109I RGGNCCY 3 cut(s) 25, 195, 839
EcoRII CCWGG 2 cut(s) 39, 1468
EcoRV GATATC 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 956
EcoT22I ATGCAT 1 cut(s) 1177
ErhI CCWWGG 1 cut(s) 956
Esp3I CGTCTC 2 cut(s) 1154, 1240
FaeI CATG 7 cut(s) 412, 613, 850, 880, 960, 1282, 1430
FalI AAGNNNNNCTT 2 cut(s) 826, 858
FaqI GGGAC 3 cut(s) 160, 1244, 1455
FatI CATG 7 cut(s) 408, 609, 846, 876, 956, 1278, 1426
FauNDI CATATG 2 cut(s) 401, 1267
FbaI TGATCA 1 cut(s) 1543
Fnu4HI GCNGC 4 cut(s) 245, 754, 1086, 1218
FokI GGATG 6 cut(s) 17, 65, 352, 713, 809, 1013
Fsp4HI GCNGC 4 cut(s) 245, 754, 1086, 1218
FspBI CTAG 7 cut(s) 192, 708, 1037, 1052, 1337, 1452, 1484
GluI GCNGC 4 cut(s) 245, 754, 1086, 1218
GsuI CTGGAG 2 cut(s) 1110, 1414
HaeIII GGCC 3 cut(s) 39, 840, 1299
HapII CCGG 3 cut(s) 29, 105, 208
Hin1II CATG 7 cut(s) 412, 613, 850, 880, 960, 1282, 1430
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 2 cut(s) 746, 1134
HinfI GANTC 5 cut(s) 201, 596, 655, 980, 1317
HpaI GTTAAC 1 cut(s) 820
HpaII CCGG 3 cut(s) 29, 105, 208
HphI GGTGA 4 cut(s) 58, 196, 430, 1162
Hpy166II GTNNAC 2 cut(s) 688, 820
Hpy188I TCNGA 8 cut(s) 387, 744, 777, 792, 985, 1180, 1362, 1412
Hpy8I GTNNAC 2 cut(s) 688, 820
HpyAV CCTTC 7 cut(s) 43, 109, 124, 481, 863, 991, 1289
HpyCH4III ACNGT 2 cut(s) 1423, 1557
HpyCH4IV ACGT 2 cut(s) 1163, 1233
HpyCH4V TGCA 8 cut(s) 263, 268, 422, 688, 897, 909, 1175, 1430
HpyF10VI GCNNNNNNNGC 2 cut(s) 713, 1046
HpyF3I CTNAG 5 cut(s) 453, 570, 900, 1388, 1512
HpySE526I ACGT 2 cut(s) 1163, 1233
Hsp92II CATG 7 cut(s) 412, 613, 850, 880, 960, 1282, 1430
Ksp22I TGATCA 1 cut(s) 1543
KspAI GTTAAC 1 cut(s) 820
Kzo9I GATC 6 cut(s) 16, 392, 1060, 1180, 1357, 1543
LmnI GCTCC 2 cut(s) 790, 1395
Lsp1109I GCAGC 3 cut(s) 231, 740, 1072
LweI GCATC 5 cut(s) 378, 694, 735, 1162, 1184
MaeI CTAG 7 cut(s) 192, 708, 1037, 1052, 1337, 1452, 1484
MaeII ACGT 2 cut(s) 1163, 1233
MaeIII GTNAC 4 cut(s) 404, 436, 1247, 1521
MalI GATC 6 cut(s) 18, 394, 1062, 1182, 1359, 1545
MboI GATC 6 cut(s) 16, 392, 1060, 1180, 1357, 1543
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 2 cut(s) 1180, 1357
MhlI GDGCHC 2 cut(s) 690, 1051
MlsI TGGCCA 1 cut(s) 39
MluNI TGGCCA 1 cut(s) 39
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 1 cut(s) 438
Mox20I TGGCCA 1 cut(s) 39
Mph1103I ATGCAT 1 cut(s) 1177
MroXI GAANNNNTTC 2 cut(s) 738, 1137
MscI TGGCCA 1 cut(s) 39
MseI TTAA 6 cut(s) 75, 431, 554, 819, 1016, 1043
Msp20I TGGCCA 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 1532
MspI CCGG 3 cut(s) 29, 105, 208
MspR9I CCNGG 4 cut(s) 29, 41, 208, 1470
MunI CAATTG 1 cut(s) 417
MvaI CCWGG 2 cut(s) 41, 1470
MwoI GCNNNNNNNGC 2 cut(s) 713, 1046
NciI CCSGG 2 cut(s) 29, 208
NcoI CCATGG 1 cut(s) 956
NdeI CATATG 2 cut(s) 401, 1267
NdeII GATC 6 cut(s) 16, 392, 1060, 1180, 1357, 1543
NlaIII CATG 7 cut(s) 412, 613, 850, 880, 960, 1282, 1430
NlaIV GGNNCC 5 cut(s) 27, 197, 1122, 1384, 1467
NmeAIII GCCGAG 1 cut(s) 187
NmuCI GTSAC 4 cut(s) 404, 436, 1247, 1521
NsiI ATGCAT 1 cut(s) 1177
PdmI GAANNNNTTC 2 cut(s) 738, 1137
PfeI GAWTC 4 cut(s) 596, 655, 980, 1317
PflMI CCANNNNNTGG 1 cut(s) 1268
PfoI TCCNGGA 1 cut(s) 1468
PkrI GCNGC 4 cut(s) 246, 755, 1087, 1219
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
PpuMI RGGWCCY 2 cut(s) 25, 195
PshAI GACNNNNGTC 1 cut(s) 486
PshBI ATTAAT 1 cut(s) 75
Psp5II RGGWCCY 2 cut(s) 25, 195
Psp6I CCWGG 2 cut(s) 39, 1468
PspGI CCWGG 2 cut(s) 39, 1468
PspN4I GGNNCC 5 cut(s) 27, 197, 1122, 1384, 1467
PspPI GGNCC 5 cut(s) 25, 195, 627, 839, 850
PspPPI RGGWCCY 2 cut(s) 25, 195
PsuI RGATCY 2 cut(s) 1180, 1357
PvuII CAGCTG 1 cut(s) 1532
RsaI GTAC 4 cut(s) 92, 170, 866, 1373
RsaNI GTAC 4 cut(s) 91, 169, 865, 1372
SaqAI TTAA 6 cut(s) 75, 431, 554, 819, 1016, 1043
SatI GCNGC 4 cut(s) 245, 754, 1086, 1218
Sau3AI GATC 6 cut(s) 16, 392, 1060, 1180, 1357, 1543
Sau96I GGNCC 5 cut(s) 25, 195, 627, 839, 850
ScaI AGTACT 2 cut(s) 92, 170
SchI GAGTC 1 cut(s) 195
ScrFI CCNGG 4 cut(s) 29, 41, 208, 1470
SduI GDGCHC 2 cut(s) 690, 1051
SfaNI GCATC 5 cut(s) 378, 694, 735, 1162, 1184
SinI GGWCC 4 cut(s) 25, 195, 627, 850
SmiI ATTTAAAT 1 cut(s) 432
SmlI CTYRAG 2 cut(s) 20, 86
SmoI CTYRAG 2 cut(s) 20, 86
SsiI CCGC 3 cut(s) 224, 484, 1217
SspMI CTAG 7 cut(s) 192, 708, 1037, 1052, 1337, 1452, 1484
StyD4I CCNGG 4 cut(s) 27, 39, 206, 1468
StyI CCWWGG 1 cut(s) 956
SwaI ATTTAAAT 1 cut(s) 432
TaaI ACNGT 2 cut(s) 1423, 1557
TaiI ACGT 2 cut(s) 1166, 1236
TaqI TCGA 5 cut(s) 229, 507, 913, 1205, 1238
TatI WGTACW 3 cut(s) 90, 168, 1371
TauI GCSGC 1 cut(s) 1220
TfiI GAWTC 4 cut(s) 596, 655, 980, 1317
Tru1I TTAA 6 cut(s) 75, 431, 554, 819, 1016, 1043
Tru9I TTAA 6 cut(s) 75, 431, 554, 819, 1016, 1043
TscAI CASTG 2 cut(s) 904, 1530
TseFI GTSAC 4 cut(s) 404, 436, 1247, 1521
TseI GCWGC 3 cut(s) 244, 753, 1085
Tsp45I GTSAC 4 cut(s) 404, 436, 1247, 1521
TspDTI ATGAA 7 cut(s) 128, 155, 354, 511, 835, 845, 1415
TspGWI ACGGA 1 cut(s) 818
TspRI CASTG 2 cut(s) 904, 1530
Van91I CCANNNNNTGG 1 cut(s) 1268
VneI GTGCAC 1 cut(s) 686
VpaK11BI GGWCC 4 cut(s) 25, 195, 627, 850
VspI ATTAAT 1 cut(s) 75
XapI RAATTY 3 cut(s) 549, 779, 1144
XbaI TCTAGA 1 cut(s) 1336
XcmI CCANNNNNNNNNTGG 1 cut(s) 1376
XmnI GAANNNNTTC 2 cut(s) 738, 1137
XspI CTAG 7 cut(s) 192, 708, 1037, 1052, 1337, 1452, 1484
ZrmI AGTACT 2 cut(s) 92, 170
Zsp2I ATGCAT 1 cut(s) 1177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.