FvH4_4g29800

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009465
Physical Location & Seq
Reverse (-)
29767754 .. 29775821
8068 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29800.t1

Sequence Viewer

Length: 2394 bp
ATGGATCCGACCCGGACCCGGAAAGTCCCTCCGAAACCCGACCTCTACTCCACCGTAGTCGTCCACGGCGACGACGAATCCGCCTCCGATTCCGACCGCCGCGCCAAGTCACCAACGGACCCGGAACCCGACCCGTACGCCACGTTCGTCTACAAGAACAATGCTCACGACGACGACGATGATGACGACGACGACGACGACGCCTCCCTCCCTCCGCTCCTCAAGCGCCTTCCCAAGGACTTCGGCGGCGGCGCCTCCCTCAATTACTTCGACGACGAGGACGACACCGCCGGCGATTTCGGGACCATGATCGTCCGCAATCGGACCACGCGGAGGTCACGTGACTGGGGGAGCCGTGCGGCCGAGGAGGAGGAGGACGACGACGGCGGAGATGCGTTCTCGACGTTTGTGATGAGGCCGAGTGAGAGAGAGTCGTCGCTGACCGGGACGGTGAGGAGGAACAGCGCCGGCGGGAATTCTACTATGAGCAGAGCCGTCGCGAGTATGCAGGCCTCCGGCGACGGGATGGGGAAGCAGAGGAAGAGCAGCGCCGCCTCGTCGCAGAACGAGGAGGCTCGCCAGAATATGAAAATGTCGACCAGTTCCATACCGGAAAGCGTCACCAGAGAAGATCCGACTACGAAGTACGAGCTGCTCAATGAGCTCGGGAAGGGGTCTTATGGTGCGGTTTATAAAGCAAGGGACTTGAAGACTTCCGAGATGGTGGCGATCAAAGTCATTTCATTATCTCAAGGGGAGGAGGGGTATGAAGAGATTTGCGGTGAAATCGAGATGCTGCAGCAATGCAGTCATCCGAATGTTGTTCGGTACTTGGGGAGCTACCAAGGAGAAGAGTATCTTTGGATTGTGATGGAGTATTGTGGAGGTGGAAGTGTTGCTGACTTGATGAATGTTACCGAGGATGCTTTGGAGGAGTATCAAATAGCGTACATTTGCAGGGAAGCATTGAAGGGCCTTGCTTATTTGCACTCGATTTTCAAGGTCCACAGAGATATTAAGGGTGGTAATATTCTGTTAACTGAACAAGGAGACGTCAAGTTGGGTGATTTTGGGGTTGCAGCACAGCTTACAAGGACCATGTCAAAGCGCAATACGTTCATTGGGACTCCACATTGGATGGCTCCCGAAGTAATTCAGGAAAGTCGTTATGATGGAAAGGTGGATGTATGGGCTCTTGGTGTTTCTGCAATTGAAATGGCTGAGGGACTTCCTCCAAGATCTGCAGTGCATCCAATGAGGGTTCTATTCATGATATCCCTTGAGCCAGCTCCAATGCTTGAGGATAAAGAAAAATGGTCTCTCGTGTTTCATGATTTTGTGGCGAAGTGCCTGACAAAAGAACCACGTTTACGCCCTACTGCAGCTGAGATGTTGAAGCACAAATTCATTGAAAAATGCAAATGTGGTCCCTCTGCAATGGTGCCTAGGATTGAGAAGGCCAGGCAAATCAGGACCTCTATGGCTTTGCAAGCACAAAATGATGCTCCAGATGAATTGGAAGATGATGGAGCTCCCAAAGTGAATGAAGATTATGGAGATACTGTTCCTTCAAGGCCTCTGGGGGCAAATGAAGTGCCTACAGGCAGCACATTGATGAAGCAGCACATGTCTGGTATTGGGGAGCATGCAGAGGAAGGTAATTTTGGCACCGTAATAGTTCATGGTGGAGATGAAAGAGATGTGACTGGTACTCGGACACAAATTTTTGATTCCAAAGAACATTCATCTGCTTCTGGACTTGGTCTTTCCACTGCTGGCGTAGGTAAATCAAGCGAAGTCAGGTTGGGAGGTGAAAATAACACTTCGGTTGGAGTACCAAATACAGTTACACAGACGAAACATGCATTTTCTCTATCACCTTTTAGCTCTCCTGAGCCGAACTTGAGAAAAAGCAGATCTCAGCCACAGGTCGGAAGTCTAACTATAAGTGGTGGCACAGTGAAGAATGAAACTGTCAGCCGGAAAGCTTTTGCAGTGCAAGATAAGCTTTGGGCCATTTATGCAGCTGGTAATACAGTACCTATTCCATTTTTGAGGGCGACAGATATATCCCCCATTGCTCTCTTATCAGATAATGTTCTCGGAGGCATGCAGCGAGATAATAGTGGAGCCGGTGCTATAGATGCACTACAGGAGCTTTTTACTGGTGATGCACAGTCAAAAAAGGGACGAAGAGGACAAAATGAGATTCCCCTTCCTCCAAGTGTTTACAAAAGACTCCATTCAAGTCCAACTCTATTGAATCTCACACAAGCTTTAGCCTACCACAAGATGTGCTACGAAGATATGCCTCTCCAAGAATTGCAAGCAACTCAGGAACAACAGACCATTCAAAATCTTTGCGATACATTAAGAACCATTCTTCGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006950 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007266 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0009893 GO:0009966 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0012501 GO:0016043 GO:0016301 GO:0016310 GO:0016477 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017048 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023051 GO:0023052 GO:0030029 GO:0030036 GO:0031098 GO:0031267 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0040011 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043408 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048365 GO:0048518 GO:0048522 GO:0048583 GO:0048870 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051674 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902531
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

798

Amino Acids

87.29

Weight (kDa)

5.12

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 216 - 470 9.1e-69 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 218 - 466 4.2e-50 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 289 - 367 1.4e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AatII GACGTC 1 cut(s) 1056
AccB1I GGYRCC 3 cut(s) 251, 1441, 1667
AccBSI CCGCTC 1 cut(s) 217
AccI GTMKAC 2 cut(s) 150, 596
AccII CGCG 3 cut(s) 102, 331, 500
AclWI GGATC 2 cut(s) 12, 626
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 3 cut(s) 475, 1403, 1722
AcvI CACGTG 1 cut(s) 341
AcyI GRCGYC 3 cut(s) 201, 252, 1053
AfaI GTAC 7 cut(s) 137, 647, 830, 950, 1711, 1836, 2040
AfiI CCNNNNNNNGG 5 cut(s) 18, 235, 333, 522, 1931
AflIII ACRYGT 1 cut(s) 1626
AjnI CCWGG 1 cut(s) 1460
AjuI GAANNNNNNNTTGG 2 cut(s) 1647, 1679
AloI GAACNNNNNNTCC 2 cut(s) 1548, 1580
Alw21I GWGCWC 2 cut(s) 666, 1534
Alw26I GTCTC 2 cut(s) 1044, 1323
AlwI GGATC 2 cut(s) 12, 626
Ama87I CYCGRG 1 cut(s) 665
AoxI GGCC 7 cut(s) 360, 416, 510, 973, 1458, 1574, 2013
ApoI RAATTY 3 cut(s) 475, 1403, 1722
Asp700I GAANNNNTTC 1 cut(s) 1152
AspA2I CCTAGG 1 cut(s) 1445
AspLEI GCGC 6 cut(s) 104, 228, 254, 467, 551, 1110
AsuC2I CCSGG 4 cut(s) 13, 19, 122, 445
AsuHPI GGTGA 8 cut(s) 102, 463, 613, 794, 1076, 1823, 1869, 2180
AvaI CYCGRG 1 cut(s) 665
AvaII GGWCC 8 cut(s) 15, 118, 303, 324, 1003, 1095, 1427, 1473
AvrII CCTAGG 1 cut(s) 1445
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 3 cut(s) 251, 1441, 1667
BanII GRGCYC 3 cut(s) 666, 1195, 1534
BauI CACGAG 1 cut(s) 1322
BbrPI CACGTG 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 716
Bbv12I GWGCWC 2 cut(s) 666, 1534
BbvCI CCTCAGC 1 cut(s) 1221
BccI CCATC 6 cut(s) 520, 715, 865, 1132, 1166, 1520
BceAI ACGGC 4 cut(s) 82, 339, 400, 479
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BciT130I CCWGG 1 cut(s) 1462
BcnI CCSGG 4 cut(s) 13, 19, 122, 445
BcoDI GTCTC 2 cut(s) 1044, 1323
BfaI CTAG 1 cut(s) 1446
BfmI CTRYAG 6 cut(s) 797, 1242, 1380, 1599, 2139, 2150
BfoI RGCGCY 4 cut(s) 229, 255, 468, 552
BglII AGATCT 2 cut(s) 1238, 1916
BlnI CCTAGG 1 cut(s) 1445
Bme1390I CCNGG 5 cut(s) 13, 19, 122, 445, 1462
Bme18I GGWCC 8 cut(s) 15, 118, 303, 324, 1003, 1095, 1427, 1473
BmeT110I CYCGRG 1 cut(s) 665
BmrFI CCNGG 5 cut(s) 13, 19, 122, 445, 1462
BmrI ACTGGG 1 cut(s) 355
BmsI GCATC 7 cut(s) 382, 783, 913, 1258, 1492, 2134, 2161
BmuI ACTGGG 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 716
BpmI CTGGAG 1 cut(s) 1491
Bpu10I CCTNAGC 2 cut(s) 1221, 1893
BpuEI CTTGAG 5 cut(s) 206, 735, 1301, 1319, 1924
BpuMI CCSGG 4 cut(s) 13, 19, 122, 445
BsaAI YACGTR 1 cut(s) 341
BsaHI GRCGYC 3 cut(s) 201, 252, 1053
BsaI GGTCTC 1 cut(s) 1323
BsaJI CCNNGG 6 cut(s) 64, 234, 363, 844, 918, 1445
BsaWI WCCGGW 1 cut(s) 610
Bsc4I CCNNNNNNNGG 5 cut(s) 18, 235, 333, 522, 1931
Bse118I RCCGGY 3 cut(s) 290, 467, 2132
Bse1I ACTGG 4 cut(s) 350, 600, 1711, 2170
Bse3DI GCAATG 3 cut(s) 809, 1443, 2076
BseBI CCWGG 1 cut(s) 1462
BseDI CCNNGG 6 cut(s) 64, 234, 363, 844, 918, 1445
BseGI GGATG 6 cut(s) 531, 811, 928, 1143, 1189, 1249
BseLI CCNNNNNNNGG 5 cut(s) 18, 235, 333, 522, 1931
BseMI GCAATG 3 cut(s) 809, 1443, 2076
BseMII CTCAG 5 cut(s) 1212, 1377, 1884, 1934, 2348
BseNI ACTGG 4 cut(s) 350, 600, 1711, 2170
BseRI GAGGAG 8 cut(s) 209, 380, 383, 386, 469, 584, 773, 947
BseX3I CGGCCG 1 cut(s) 360
Bsh1236I CGCG 3 cut(s) 102, 331, 500
Bsh1285I CGRYCG 2 cut(s) 97, 363
BshFI GGCC 7 cut(s) 362, 418, 512, 975, 1460, 1576, 2015
BshNI GGYRCC 3 cut(s) 251, 1441, 1667
BsiEI CGRYCG 2 cut(s) 97, 363
BsiHKAI GWGCWC 2 cut(s) 666, 1534
BsiHKCI CYCGRG 1 cut(s) 665
BsiWI CGTACG 1 cut(s) 135
BslFI GGGAC 8 cut(s) 11, 316, 460, 716, 1138, 1239, 1413, 2202
BslI CCNNNNNNNGG 5 cut(s) 18, 235, 333, 522, 1931
BsmAI GTCTC 2 cut(s) 1044, 1323
BsmBI CGTCTC 1 cut(s) 1044
BsmFI GGGAC 8 cut(s) 11, 316, 460, 716, 1138, 1239, 1413, 2202
BsnI GGCC 7 cut(s) 362, 418, 512, 975, 1460, 1576, 2015
Bso31I GGTCTC 1 cut(s) 1323
BsoBI CYCGRG 1 cut(s) 665
Bsp1286I GDGCHC 3 cut(s) 666, 1195, 1534
Bsp143I GATC 6 cut(s) 4, 309, 631, 729, 1238, 1916
Bsp68I TCGCGA 1 cut(s) 500
BspANI GGCC 7 cut(s) 362, 418, 512, 975, 1460, 1576, 2015
BspCNI CTCAG 5 cut(s) 1213, 1378, 1885, 1933, 2347
BspFNI CGCG 3 cut(s) 102, 331, 500
BspHI TCATGA 2 cut(s) 1269, 1330
BspMAI CTGCAG 3 cut(s) 801, 1246, 1384
BspPI GGATC 2 cut(s) 12, 626
BspQI GCTCTTC 1 cut(s) 536
BspT107I GGYRCC 3 cut(s) 251, 1441, 1667
BspTNI GGTCTC 1 cut(s) 1323
BsrBI CCGCTC 1 cut(s) 217
BsrDI GCAATG 3 cut(s) 809, 1443, 2076
BsrFI RCCGGY 3 cut(s) 290, 467, 2132
BsrI ACTGG 4 cut(s) 350, 600, 1711, 2170
BssAI RCCGGY 3 cut(s) 290, 467, 2132
BssECI CCNNGG 6 cut(s) 64, 234, 363, 844, 918, 1445
BssMI GATC 6 cut(s) 4, 309, 631, 729, 1238, 1916
BssNI GRCGYC 3 cut(s) 201, 252, 1053
BssSI CACGAG 1 cut(s) 1322
BssT1I CCWWGG 3 cut(s) 234, 844, 1445
Bst2BI CACGAG 1 cut(s) 1322
Bst2UI CCWGG 1 cut(s) 1462
Bst4CI ACNGT 9 cut(s) 55, 451, 1564, 1672, 1846, 1960, 1975, 2038, 2178
Bst6I CTCTTC 4 cut(s) 536, 765, 846, 2188
BstACI GRCGYC 3 cut(s) 201, 252, 1053
BstBAI YACGTR 1 cut(s) 341
BstDEI CTNAG 5 cut(s) 1221, 1386, 1893, 1920, 2334
BstDSI CCRYGG 1 cut(s) 64
BstENI CCTNNNNNAGG 1 cut(s) 233
BstF5I GGATG 6 cut(s) 531, 811, 928, 1143, 1189, 1249
BstFNI CGCG 3 cut(s) 102, 331, 500
BstH2I RGCGCY 4 cut(s) 229, 255, 468, 552
BstHHI GCGC 6 cut(s) 104, 228, 254, 467, 551, 1110
BstKTI GATC 6 cut(s) 7, 312, 634, 732, 1241, 1919
BstMAI GTCTC 2 cut(s) 1044, 1323
BstMBI GATC 6 cut(s) 4, 309, 631, 729, 1238, 1916
BstMCI CGRYCG 2 cut(s) 97, 363
BstMWI GCNNNNNNNGC 6 cut(s) 223, 661, 1490, 2005, 2021, 2144
BstNI CCWGG 1 cut(s) 1462
BstNSI RCATGY 4 cut(s) 1630, 1649, 1865, 2113
BstSCI CCNGG 5 cut(s) 11, 17, 120, 443, 1460
BstSFI CTRYAG 6 cut(s) 797, 1242, 1380, 1599, 2139, 2150
BstUI CGCG 3 cut(s) 102, 331, 500
BstV2I GAAGAC 1 cut(s) 716
BstX2I RGATCY 4 cut(s) 4, 631, 1238, 1916
BstYI RGATCY 4 cut(s) 4, 631, 1238, 1916
BstZI CGGCCG 1 cut(s) 360
BsuRI GGCC 7 cut(s) 362, 418, 512, 975, 1460, 1576, 2015
BtgI CCRYGG 1 cut(s) 64
BtsCI GGATG 6 cut(s) 531, 811, 928, 1143, 1189, 1249
BtsI GCAGTG 3 cut(s) 1251, 1770, 2001
BtsIMutI CAGTG 4 cut(s) 1251, 1770, 1965, 2001
BtuMI TCGCGA 1 cut(s) 500
CciI TCATGA 2 cut(s) 1269, 1330
CfoI GCGC 6 cut(s) 104, 228, 254, 467, 551, 1110
Cfr10I RCCGGY 3 cut(s) 290, 467, 2132
CseI GACGC 2 cut(s) 209, 607
Csp6I GTAC 7 cut(s) 136, 646, 829, 949, 1710, 1835, 2039
CviAII CATG 9 cut(s) 307, 1099, 1270, 1331, 1627, 1646, 1682, 1862, 2110
CviQI GTAC 7 cut(s) 136, 646, 829, 949, 1710, 1835, 2039
DdeI CTNAG 5 cut(s) 1221, 1386, 1893, 1920, 2334
DinI GGCGCC 1 cut(s) 253
DpnI GATC 6 cut(s) 6, 311, 633, 731, 1240, 1918
DpnII GATC 6 cut(s) 4, 309, 631, 729, 1238, 1916
EaeI YGGCCR 1 cut(s) 360
EagI CGGCCG 1 cut(s) 360
Eam1104I CTCTTC 4 cut(s) 536, 765, 846, 2188
EarI CTCTTC 4 cut(s) 536, 765, 846, 2188
EciI GGCGGA 2 cut(s) 70, 402
Ecl136II GAGCTC 2 cut(s) 664, 1532
EclXI CGGCCG 1 cut(s) 360
Eco130I CCWWGG 3 cut(s) 234, 844, 1445
Eco147I AGGCCT 2 cut(s) 512, 1576
Eco24I GRGCYC 3 cut(s) 666, 1195, 1534
Eco31I GGTCTC 1 cut(s) 1323
Eco32I GATATC 1 cut(s) 1275
Eco47I GGWCC 8 cut(s) 15, 118, 303, 324, 1003, 1095, 1427, 1473
Eco52I CGGCCG 1 cut(s) 360
Eco53kI GAGCTC 2 cut(s) 664, 1532
Eco72I CACGTG 1 cut(s) 341
Eco88I CYCGRG 1 cut(s) 665
EcoICRI GAGCTC 2 cut(s) 664, 1532
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoO109I RGGNCCY 2 cut(s) 973, 1473
EcoRI GAATTC 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 1460
EcoRV GATATC 1 cut(s) 1275
EcoT14I CCWWGG 3 cut(s) 234, 844, 1445
EcoT22I ATGCAT 1 cut(s) 1867
EcoT38I GRGCYC 3 cut(s) 666, 1195, 1534
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 3 cut(s) 234, 844, 1445
Esp3I CGTCTC 1 cut(s) 1044
FaeI CATG 9 cut(s) 310, 1102, 1273, 1334, 1630, 1649, 1685, 1865, 2113
FalI AAGNNNNNCTT 4 cut(s) 843, 875, 1992, 2024
FaqI GGGAC 8 cut(s) 11, 316, 460, 716, 1138, 1239, 1413, 2202
FatI CATG 9 cut(s) 306, 1098, 1269, 1330, 1626, 1645, 1681, 1861, 2109
FauI CCCGC 1 cut(s) 464
FblI GTMKAC 2 cut(s) 150, 596
FokI GGATG 6 cut(s) 538, 798, 935, 1150, 1196, 1236
FriOI GRGCYC 3 cut(s) 666, 1195, 1534
FspBI CTAG 1 cut(s) 1446
GlaI GCGC 6 cut(s) 103, 227, 253, 466, 550, 1109
GsuI CTGGAG 1 cut(s) 1491
HaeII RGCGCY 4 cut(s) 229, 255, 468, 552
HaeIII GGCC 7 cut(s) 362, 418, 512, 975, 1460, 1576, 2015
HgaI GACGC 2 cut(s) 209, 607
HhaI GCGC 6 cut(s) 104, 228, 254, 467, 551, 1110
Hin1I GRCGYC 3 cut(s) 201, 252, 1053
Hin1II CATG 9 cut(s) 310, 1102, 1273, 1334, 1630, 1649, 1685, 1865, 2113
Hin6I GCGC 6 cut(s) 102, 226, 252, 465, 549, 1108
HinP1I GCGC 6 cut(s) 102, 226, 252, 465, 549, 1108
HincII GTYRAC 2 cut(s) 597, 1038
HindII GTYRAC 2 cut(s) 597, 1038
HindIII AAGCTT 3 cut(s) 1986, 2006, 2274
HinfI GANTC 8 cut(s) 77, 89, 431, 1126, 1730, 2209, 2238, 2263
HpaI GTTAAC 1 cut(s) 1038
HphI GGTGA 8 cut(s) 102, 463, 613, 794, 1076, 1823, 1869, 2180
Hpy166II GTNNAC 7 cut(s) 64, 151, 597, 1006, 1038, 1370, 2230
Hpy8I GTNNAC 7 cut(s) 64, 151, 597, 1006, 1038, 1370, 2230
HpyAV CCTTC 7 cut(s) 239, 664, 964, 1450, 1578, 1649, 2225
HpyCH4III ACNGT 9 cut(s) 55, 451, 1564, 1672, 1846, 1960, 1975, 2038, 2178
HpyCH4IV ACGT 6 cut(s) 143, 340, 404, 1053, 1115, 1366
HpyF10VI GCNNNNNNNGC 6 cut(s) 223, 661, 1490, 2005, 2021, 2144
HpyF3I CTNAG 5 cut(s) 1221, 1386, 1893, 1920, 2334
HpySE526I ACGT 6 cut(s) 143, 340, 404, 1053, 1115, 1366
Hsp92I GRCGYC 3 cut(s) 201, 252, 1053
Hsp92II CATG 9 cut(s) 310, 1102, 1273, 1334, 1630, 1649, 1685, 1865, 2113
HspAI GCGC 6 cut(s) 102, 226, 252, 465, 549, 1108
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 2 cut(s) 290, 467
KroNI GCCGGC 2 cut(s) 292, 469
KspAI GTTAAC 1 cut(s) 1038
Kzo9I GATC 6 cut(s) 4, 309, 631, 729, 1238, 1916
LguI GCTCTTC 1 cut(s) 536
LweI GCATC 7 cut(s) 382, 783, 913, 1258, 1492, 2134, 2161
MaeI CTAG 1 cut(s) 1446
MaeII ACGT 6 cut(s) 143, 340, 404, 1053, 1115, 1366
MaeIII GTNAC 7 cut(s) 108, 336, 341, 619, 913, 1702, 1846
MalI GATC 6 cut(s) 6, 311, 633, 731, 1240, 1918
MbiI CCGCTC 1 cut(s) 217
MboI GATC 6 cut(s) 4, 309, 631, 729, 1238, 1916
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 4 cut(s) 4, 631, 1238, 1916
MhlI GDGCHC 3 cut(s) 666, 1195, 1534
MluCI AATT 9 cut(s) 262, 475, 1152, 1209, 1403, 1514, 1660, 1722, 2321
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 3 cut(s) 440, 1120, 2232
MmeI TCCRAC 6 cut(s) 32, 117, 659, 1810, 1912, 2276
Mph1103I ATGCAT 1 cut(s) 1867
MreI CGCCGGCG 2 cut(s) 290, 467
MroNI GCCGGC 2 cut(s) 290, 467
MroXI GAANNNNTTC 1 cut(s) 1152
MseI TTAA 3 cut(s) 1017, 1037, 2372
MslI CAYNNNNRTG 2 cut(s) 816, 1613
MspA1I CMGCKG 2 cut(s) 1385, 2027
MspR9I CCNGG 5 cut(s) 13, 19, 122, 445, 1462
MunI CAATTG 1 cut(s) 1209
MvaI CCWGG 1 cut(s) 1462
MvnI CGCG 3 cut(s) 102, 331, 500
MwoI GCNNNNNNNGC 6 cut(s) 223, 661, 1490, 2005, 2021, 2144
NaeI GCCGGC 2 cut(s) 292, 469
NarI GGCGCC 1 cut(s) 252
NciI CCSGG 4 cut(s) 13, 19, 122, 445
NdeII GATC 6 cut(s) 4, 309, 631, 729, 1238, 1916
NgoMIV GCCGGC 2 cut(s) 290, 467
NlaIII CATG 9 cut(s) 310, 1102, 1273, 1334, 1630, 1649, 1685, 1865, 2113
NmeAIII GCCGAG 2 cut(s) 388, 444
NmuCI GTSAC 5 cut(s) 108, 336, 341, 619, 1702
NruI TCGCGA 1 cut(s) 500
NsiI ATGCAT 1 cut(s) 1867
NspI RCATGY 4 cut(s) 1630, 1649, 1865, 2113
PaeI GCATGC 2 cut(s) 1649, 2113
PagI TCATGA 2 cut(s) 1269, 1330
PceI AGGCCT 2 cut(s) 512, 1576
PciI ACATGT 1 cut(s) 1626
PciSI GCTCTTC 1 cut(s) 536
PcsI WCGNNNNNNNCGW 8 cut(s) 66, 72, 144, 174, 183, 192, 195, 279
PdiI GCCGGC 2 cut(s) 292, 469
PdmI GAANNNNTTC 1 cut(s) 1152
PfeI GAWTC 5 cut(s) 77, 89, 1730, 2209, 2263
Pfl23II CGTACG 1 cut(s) 135
PflFI GACNNNGTC 2 cut(s) 1099, 1761
PleI GAGTC 3 cut(s) 439, 1120, 2232
PluTI GGCGCC 1 cut(s) 255
PmaCI CACGTG 1 cut(s) 341
PmlI CACGTG 1 cut(s) 341
PpsI GAGTC 3 cut(s) 439, 1120, 2232
Ppu21I YACGTR 1 cut(s) 341
PpuMI RGGWCCY 1 cut(s) 1473
PscI ACATGT 1 cut(s) 1626
PsiI TTATAA 1 cut(s) 693
Psp124BI GAGCTC 2 cut(s) 666, 1534
Psp5II RGGWCCY 1 cut(s) 1473
Psp6I CCWGG 1 cut(s) 1460
PspCI CACGTG 1 cut(s) 341
PspGI CCWGG 1 cut(s) 1460
PspLI CGTACG 1 cut(s) 135
PspPPI RGGWCCY 1 cut(s) 1473
PsrI GAACNNNNNNTAC 4 cut(s) 128, 160, 1353, 1385
PstI CTGCAG 3 cut(s) 801, 1246, 1384
PsuI RGATCY 4 cut(s) 4, 631, 1238, 1916
PsyI GACNNNGTC 2 cut(s) 1099, 1761
PvuII CAGCTG 2 cut(s) 1385, 2027
RruI TCGCGA 1 cut(s) 500
RsaI GTAC 7 cut(s) 137, 647, 830, 950, 1711, 1836, 2040
RsaNI GTAC 7 cut(s) 136, 646, 829, 949, 1710, 1835, 2039
RseI CAYNNNNRTG 2 cut(s) 816, 1613
SacI GAGCTC 2 cut(s) 666, 1534
SalI GTCGAC 1 cut(s) 595
SapI GCTCTTC 1 cut(s) 536
SaqAI TTAA 3 cut(s) 1017, 1037, 2372
Sau3AI GATC 6 cut(s) 4, 309, 631, 729, 1238, 1916
SchI GAGTC 3 cut(s) 440, 1120, 2232
ScrFI CCNGG 5 cut(s) 13, 19, 122, 445, 1462
SduI GDGCHC 3 cut(s) 666, 1195, 1534
SfaNI GCATC 7 cut(s) 382, 783, 913, 1258, 1492, 2134, 2161
SfcI CTRYAG 6 cut(s) 797, 1242, 1380, 1599, 2139, 2150
SfoI GGCGCC 1 cut(s) 253
SgrAI CRCCGGYG 2 cut(s) 290, 467
SinI GGWCC 8 cut(s) 15, 118, 303, 324, 1003, 1095, 1427, 1473
SmiMI CAYNNNNRTG 2 cut(s) 816, 1613
SmlI CTYRAG 5 cut(s) 221, 750, 1280, 1298, 1903
SmoI CTYRAG 5 cut(s) 221, 750, 1280, 1298, 1903
SphI GCATGC 2 cut(s) 1649, 2113
Sse9I AATT 9 cut(s) 262, 475, 1152, 1209, 1403, 1514, 1660, 1722, 2321
SseBI AGGCCT 2 cut(s) 512, 1576
SspDI GGCGCC 1 cut(s) 251
SspI AATATT 1 cut(s) 1030
SspMI CTAG 1 cut(s) 1446
SstI GAGCTC 2 cut(s) 666, 1534
StuI AGGCCT 2 cut(s) 512, 1576
StyD4I CCNGG 5 cut(s) 11, 17, 120, 443, 1460
StyI CCWWGG 3 cut(s) 234, 844, 1445
TaaI ACNGT 9 cut(s) 55, 451, 1564, 1672, 1846, 1960, 1975, 2038, 2178
TaiI ACGT 6 cut(s) 146, 343, 407, 1056, 1118, 1369
TaqI TCGA 5 cut(s) 270, 401, 596, 789, 992
TasI AATT 9 cut(s) 262, 475, 1152, 1209, 1403, 1514, 1660, 1722, 2321
TauI GCSGC 5 cut(s) 102, 249, 252, 362, 554
TfiI GAWTC 5 cut(s) 77, 89, 1730, 2209, 2263
Tru1I TTAA 3 cut(s) 1017, 1037, 2372
Tru9I TTAA 3 cut(s) 1017, 1037, 2372
TscAI CASTG 4 cut(s) 1251, 1777, 1965, 2001
TseFI GTSAC 5 cut(s) 108, 336, 341, 619, 1702
Tsp45I GTSAC 5 cut(s) 108, 336, 341, 619, 1702
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 4 cut(s) 1251, 1777, 1965, 2001
Tth111I GACNNNGTC 2 cut(s) 1099, 1761
VpaK11BI GGWCC 8 cut(s) 15, 118, 303, 324, 1003, 1095, 1427, 1473
XagI CCTNNNNNAGG 1 cut(s) 233
XapI RAATTY 3 cut(s) 475, 1403, 1722
XceI RCATGY 4 cut(s) 1630, 1649, 1865, 2113
XmaJI CCTAGG 1 cut(s) 1445
XmiI GTMKAC 2 cut(s) 150, 596
XmnI GAANNNNTTC 1 cut(s) 1152
XspI CTAG 1 cut(s) 1446
ZraI GACGTC 1 cut(s) 1054
Zsp2I ATGCAT 1 cut(s) 1867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.