Rh4CG382900

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009465
Physical Location & Seq
Reverse (-)
63615170 .. 63622809
7640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG382900.1

Sequence Viewer

Length: 2397 bp
ATGGATCCGACCGGCACCCCTCGCCGGACCCGAAATATCCCGCCGAAACCCGACCTCTACTCCACCGTAGTCGTCCACGGCGACGACGATTCCACCTCCGAGTCCGACCGAGACCGGCGGCGCGGCAACCAGGAACCCGACCCGTACGCCACATTCGTATACAAGAACAATGCTCACGACGAAGACGACGAAGACGACTCCTCACTCCCTCCCCTCCTCAAACGCCTCCCCAAGGACTTCGGCGGCGGCGCCTCCCTCAACTACTTCGACGACGAGGACGACAACGGCGGCGATTTCGGCACCGTGATCGTCAAGAGCGACCGCAATCGGACCACCAGGAGGTCGTCACGTGACTGGGGGAGCCGAGCCGCGGAGCGGGACGACGGTGACGGCGGAGATGCGTTCTCGACGTTTGTGATGAGGCCGAGTGAGAGAGAGTCGTCGCTGACCGGGACGGTGAGGAGGACCAGCGGCGGCAGTTCCACGATGAGCAGGGCGGTGGCGAGTATGCAGGCCTCCGGCGACGGGATGGGGAAGCAGAGGAAGAGCAGTGCCGCCTCGTCGCAAAACGAGGAGGCTCGCCAGAATATGAAAATGTCGACCAGTTCCATACCGGAAAGCGTCACCAGAGAAGATCCGACTACAAAGTACGAGTTGCTCAATGAGCTTGGGAAGGGGTCTTATGGTGCGGTTTATAAAGCTAGGGACTTAAAGACTTCCGAGCTGGTGGCAATTAAAGTCATTTCATTATCTCAAGGGGAGGAAGGGTATGAAGAGATTTGTGGTGAAATTGAGATGCTGCAGCAATGCAGTCATCCGAATGTCGTTCGGTACTTGGGGAGCTACCAAGGAGAAGAGTATCTTTGGATTGTGATGGAGTACTGTGGTGGTGGAAGTGTTGCTGACTTAATGAATGTTACCGAGGATGCTTTGGAGGAGTATCAAATAGCTTACATTTGCAGGGAAGCATTGAAGGGCCTTGCTTATTTGCACTCAATTTTCAAGGTCCACAGAGATATTAAGGGTGGTAATATTCTGTTAACTGAACAAGGAGATGTCAAGTTGGGTGACTTTGGGGTTGCAGCACAGCTTACAAGGACCATGTCAAAGCGCAATACGTTCATTGGGACTCCACATTGGATGGCTCCAGAAGTTATTCAGGAAAGCCGCTATGATGGAAAGGTGGATGTATGGGCTCTTGGTGTTTCTGCAATTGAAATGGCTGAGGGACTTCCTCCAAGATCTGCGGTGCATCCAATGAGGGTTCTATTCATGATATCCCTTGAGCCAGCTCCGATGCTTGAGGATAAAGAAAAATGGTCTCTCGTGTTTCATGATTTTGTGGCGAAGTGCCTGACAAAAGAACCTCGGTTACGCCCTACTGCAGCTGAGATGTTGAAGCACAAATTCATCGAAAAATGCAAATGTGGTCCCTCTGCAATGGTGCCAAGGATTGAGAAGGCCAGGCAAATTAGGACCTCTATGGCTTTGCAAGCACAAAATGAAGCTCCAGATGAACAGGAAGACGATACACTTGGAGCTCCCAAAGTGAATGAAGATTATGGAGATACCGTTCCTTCAAGGCCTCTAGGGGCAAATGAAGTGACAGGCAGCACATTGACGAAGCAGCACATGTCTGGTATTGGGGAGCATGCAGAGGAAGGTAATTATGGCACCGTAATAATTCATGGTGGAGATGAAAGAGATGAGACTGGTACTCGGACACAAATTTTTAATTCCAAAGAAGATTCACCTGCCTCTGGACTTGGCCCTTCCACTGCTGGTACAGTAGGTAAATCAAGCACAGTCAGGGTGGGAGACGAAAATAACACCTCGGTTGGAGTACGAAATACAGTTACAGAGGCCAAACCAGCATTTTCTCTTTCAACCTTTACCTCTTCTGAGCCGAACTTGAGAAAATGCAAATCTCAACCACAGGTTGGAAGTCAAAGCGGTGGCACAGTGAAGAATGAAACTTTCAGCCGGAAAGCTTTTGCAGTGCAAGATAAGCTTTGGTCCATATATGCAGCTGGTAATACAGTGCCTATTCCATTTTTGAGGGCAACAGATATATCCCCCATTGCTCTCTTATCAGATAATGTTGTTGGAGGCATGCAACGAGATCATAGTGGAACTGGTGCTATAGATGCACTACAGGAGCTTTTTACTGGCGATGCACAGTCTAAAAAGGGACGAAGAGGGCAAAATGAGATGCCCCTTCCTCCAAGTGTTTACAAAAGACTCTTTGCAAGTCCAACTCTATTAAATCTCACACAAGCTTTAGCCTACCACAAGATGTGCTATGAAGATATGCCTCTCCAAGAATTGCAAGCAACTCAGGAACAACAGACTATTCAAAATCTTTGCGATACACTAAGAACCATTCTTCGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006950 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007266 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0009893 GO:0009966 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0012501 GO:0016043 GO:0016301 GO:0016310 GO:0016477 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017048 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023051 GO:0023052 GO:0030029 GO:0030036 GO:0031098 GO:0031267 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0040011 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043408 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048365 GO:0048518 GO:0048522 GO:0048583 GO:0048870 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051674 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902531
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

798

Amino Acids

87.59

Weight (kDa)

5.33

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 217 - 471 1.3e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 219 - 467 7.3e-50 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 290 - 368 1.4e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AarI CACCTGC 1 cut(s) 1762
Acc36I ACCTGC 1 cut(s) 1762
AccB1I GGYRCC 5 cut(s) 14, 248, 299, 1444, 1673
AccB7I CCANNNNNTGG 1 cut(s) 1940
AccBSI CCGCTC 1 cut(s) 376
AccI GTMKAC 2 cut(s) 159, 599
AccII CGCG 2 cut(s) 123, 371
AclWI GGATC 2 cut(s) 12, 629
AcsI RAATTY 2 cut(s) 1406, 1728
AcvI CACGTG 1 cut(s) 350
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 7 cut(s) 146, 650, 833, 881, 1717, 1786, 1845
AfiI CCNNNNNNNGG 8 cut(s) 24, 232, 339, 370, 375, 525, 1760, 1940
AflIII ACRYGT 1 cut(s) 1632
AgsI TTSAA 7 cut(s) 973, 1003, 1217, 1399, 1581, 1887, 2357
AjnI CCWGG 3 cut(s) 129, 335, 1463
AloI GAACNNNNNNTCC 2 cut(s) 1557, 1589
Alw21I GWGCWC 1 cut(s) 1543
Alw26I GTCTC 4 cut(s) 105, 1326, 1703, 1812
AlwI GGATC 2 cut(s) 12, 629
AoxI GGCC 7 cut(s) 422, 513, 976, 1461, 1583, 1768, 1863
ApeKI GCWGC 7 cut(s) 799, 802, 1082, 1385, 1611, 1627, 2027
ApoI RAATTY 2 cut(s) 1406, 1728
Asp700I GAANNNNTTC 1 cut(s) 1155
AspLEI GCGC 3 cut(s) 123, 251, 1113
AsuC2I CCSGG 1 cut(s) 451
AsuHPI GGTGA 6 cut(s) 398, 469, 616, 797, 1079, 1743
AvaII GGWCC 8 cut(s) 27, 330, 465, 1006, 1098, 1430, 1476, 2016
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 5 cut(s) 14, 248, 299, 1444, 1673
BanII GRGCYC 2 cut(s) 1198, 1543
BauI CACGAG 1 cut(s) 1325
BbrPI CACGTG 1 cut(s) 350
BbsI GAAGAC 3 cut(s) 189, 198, 1530
Bbv12I GWGCWC 1 cut(s) 1543
BbvCI CCTCAGC 1 cut(s) 1224
BbvI GCAGC 7 cut(s) 786, 814, 1094, 1397, 1623, 1639, 2039
BccI CCATC 4 cut(s) 523, 868, 1135, 1169
BceAI ACGGC 3 cut(s) 94, 301, 406
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BciT130I CCWGG 3 cut(s) 131, 337, 1465
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 4 cut(s) 105, 1326, 1703, 1812
BfaI CTAG 2 cut(s) 702, 1589
BfmI CTRYAG 4 cut(s) 800, 1383, 2142, 2153
BfoI RGCGCY 1 cut(s) 252
BfuAI ACCTGC 1 cut(s) 1762
BglII AGATCT 1 cut(s) 1241
BmcAI AGTACT 1 cut(s) 881
Bme1390I CCNGG 4 cut(s) 131, 337, 451, 1465
Bme18I GGWCC 8 cut(s) 27, 330, 465, 1006, 1098, 1430, 1476, 2016
BmrFI CCNGG 4 cut(s) 131, 337, 451, 1465
BmrI ACTGGG 1 cut(s) 364
BmsI GCATC 8 cut(s) 388, 786, 916, 1261, 1287, 2137, 2164, 2202
BmuI ACTGGG 1 cut(s) 364
BpiI GAAGAC 3 cut(s) 189, 198, 1530
BpmI CTGGAG 2 cut(s) 1131, 1494
Bpu10I CCTNAGC 1 cut(s) 1224
BpuEI CTTGAG 4 cut(s) 738, 1304, 1322, 1933
BpuMI CCSGG 1 cut(s) 451
BsaAI YACGTR 1 cut(s) 350
BsaHI GRCGYC 1 cut(s) 249
BsaI GGTCTC 2 cut(s) 105, 1326
BsaJI CCNNGG 8 cut(s) 76, 231, 369, 847, 921, 1367, 1448, 1833
BsaWI WCCGGW 1 cut(s) 613
BsaXI ACNNNNNCTCC 6 cut(s) 44, 74, 752, 782, 1557, 1587
Bsc4I CCNNNNNNNGG 8 cut(s) 24, 232, 339, 370, 375, 525, 1760, 1940
Bse118I RCCGGY 2 cut(s) 11, 114
Bse1I ACTGG 5 cut(s) 359, 603, 1717, 2140, 2173
Bse3DI GCAATG 3 cut(s) 812, 1446, 2079
BseBI CCWGG 3 cut(s) 131, 337, 1465
BseDI CCNNGG 8 cut(s) 76, 231, 369, 847, 921, 1367, 1448, 1833
BseGI GGATG 6 cut(s) 534, 814, 931, 1146, 1192, 1252
BseLI CCNNNNNNNGG 8 cut(s) 24, 232, 339, 370, 375, 525, 1760, 1940
BseMI GCAATG 3 cut(s) 812, 1446, 2079
BseMII CTCAG 4 cut(s) 1215, 1380, 1893, 2351
BseNI ACTGG 5 cut(s) 359, 603, 1717, 2140, 2173
BseRI GAGGAG 5 cut(s) 190, 206, 475, 587, 950
BseXI GCAGC 7 cut(s) 786, 814, 1094, 1397, 1623, 1639, 2039
Bsh1236I CGCG 2 cut(s) 123, 371
Bsh1285I CGRYCG 3 cut(s) 12, 109, 322
BshFI GGCC 7 cut(s) 424, 515, 978, 1463, 1585, 1770, 1865
BshNI GGYRCC 5 cut(s) 14, 248, 299, 1444, 1673
BsiEI CGRYCG 3 cut(s) 12, 109, 322
BsiHKAI GWGCWC 1 cut(s) 1543
BsiSI CCGG 7 cut(s) 12, 25, 115, 450, 519, 614, 1984
BsiWI CGTACG 1 cut(s) 144
BslFI GGGAC 7 cut(s) 392, 466, 719, 1141, 1242, 1416, 2205
BslI CCNNNNNNNGG 8 cut(s) 24, 232, 339, 370, 375, 525, 1760, 1940
BsmAI GTCTC 4 cut(s) 105, 1326, 1703, 1812
BsmBI CGTCTC 1 cut(s) 1812
BsmFI GGGAC 7 cut(s) 392, 466, 719, 1141, 1242, 1416, 2205
BsnI GGCC 7 cut(s) 424, 515, 978, 1463, 1585, 1770, 1865
Bso31I GGTCTC 2 cut(s) 105, 1326
Bsp1286I GDGCHC 2 cut(s) 1198, 1543
Bsp143I GATC 5 cut(s) 4, 306, 634, 1241, 2122
BspANI GGCC 7 cut(s) 424, 515, 978, 1463, 1585, 1770, 1865
BspCNI CTCAG 4 cut(s) 1216, 1381, 1894, 2350
BspFNI CGCG 2 cut(s) 123, 371
BspHI TCATGA 2 cut(s) 1272, 1333
BspMAI CTGCAG 2 cut(s) 804, 1387
BspMI ACCTGC 1 cut(s) 1762
BspPI GGATC 2 cut(s) 12, 629
BspQI GCTCTTC 1 cut(s) 539
BspT107I GGYRCC 5 cut(s) 14, 248, 299, 1444, 1673
BspTNI GGTCTC 2 cut(s) 105, 1326
BsrBI CCGCTC 1 cut(s) 376
BsrDI GCAATG 3 cut(s) 812, 1446, 2079
BsrFI RCCGGY 2 cut(s) 11, 114
BsrI ACTGG 5 cut(s) 359, 603, 1717, 2140, 2173
BssAI RCCGGY 2 cut(s) 11, 114
BssECI CCNNGG 8 cut(s) 76, 231, 369, 847, 921, 1367, 1448, 1833
BssMI GATC 5 cut(s) 4, 306, 634, 1241, 2122
BssNAI GTATAC 1 cut(s) 160
BssNI GRCGYC 1 cut(s) 249
BssSI CACGAG 1 cut(s) 1325
BssT1I CCWWGG 3 cut(s) 231, 847, 1448
Bst1107I GTATAC 1 cut(s) 160
Bst2BI CACGAG 1 cut(s) 1325
Bst2UI CCWGG 3 cut(s) 131, 337, 1465
Bst6I CTCTTC 5 cut(s) 539, 768, 849, 1903, 2191
BstACI GRCGYC 1 cut(s) 249
BstBAI YACGTR 1 cut(s) 350
BstC8I GCNNGC 7 cut(s) 513, 580, 1290, 1494, 1653, 2114, 2331
BstDEI CTNAG 5 cut(s) 1224, 1389, 1902, 2337, 2375
BstDSI CCRYGG 2 cut(s) 76, 369
BstENI CCTNNNNNAGG 1 cut(s) 230
BstF5I GGATG 6 cut(s) 534, 814, 931, 1146, 1192, 1252
BstFNI CGCG 2 cut(s) 123, 371
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 3 cut(s) 123, 251, 1113
BstKTI GATC 5 cut(s) 7, 309, 637, 1244, 2125
BstMAI GTCTC 4 cut(s) 105, 1326, 1703, 1812
BstMBI GATC 5 cut(s) 4, 306, 634, 1241, 2122
BstMCI CGRYCG 3 cut(s) 12, 109, 322
BstMWI GCNNNNNNNGC 7 cut(s) 21, 297, 664, 1493, 1871, 2008, 2147
BstNI CCWGG 3 cut(s) 131, 337, 1465
BstNSI RCATGY 3 cut(s) 1636, 1655, 2116
BstSCI CCNGG 4 cut(s) 129, 335, 449, 1463
BstSFI CTRYAG 4 cut(s) 800, 1383, 2142, 2153
BstUI CGCG 2 cut(s) 123, 371
BstV1I GCAGC 7 cut(s) 786, 814, 1094, 1397, 1623, 1639, 2039
BstV2I GAAGAC 3 cut(s) 189, 198, 1530
BstX2I RGATCY 3 cut(s) 4, 634, 1241
BstYI RGATCY 3 cut(s) 4, 634, 1241
BstZ17I GTATAC 1 cut(s) 160
BsuRI GGCC 7 cut(s) 424, 515, 978, 1463, 1585, 1770, 1865
BtgI CCRYGG 2 cut(s) 76, 369
BtgZI GCGATG 1 cut(s) 2187
BtsCI GGATG 6 cut(s) 534, 814, 931, 1146, 1192, 1252
BtsI GCAGTG 3 cut(s) 556, 1776, 2004
BtsIMutI CAGTG 5 cut(s) 556, 1776, 1968, 2004, 2046
BveI ACCTGC 1 cut(s) 1762
Cac8I GCNNGC 7 cut(s) 513, 580, 1290, 1494, 1653, 2114, 2331
CciI TCATGA 2 cut(s) 1272, 1333
CfoI GCGC 3 cut(s) 123, 251, 1113
Cfr10I RCCGGY 2 cut(s) 11, 114
Cfr42I CCGCGG 1 cut(s) 372
CseI GACGC 1 cut(s) 610
Csp6I GTAC 7 cut(s) 145, 649, 832, 880, 1716, 1785, 1844
CviAII CATG 7 cut(s) 1102, 1273, 1334, 1633, 1652, 1688, 2113
CviQI GTAC 7 cut(s) 145, 649, 832, 880, 1716, 1785, 1844
DdeI CTNAG 5 cut(s) 1224, 1389, 1902, 2337, 2375
DinI GGCGCC 1 cut(s) 250
DpnI GATC 5 cut(s) 6, 308, 636, 1243, 2124
DpnII GATC 5 cut(s) 4, 306, 634, 1241, 2122
Eam1104I CTCTTC 5 cut(s) 539, 768, 849, 1903, 2191
EarI CTCTTC 5 cut(s) 539, 768, 849, 1903, 2191
EciI GGCGGA 1 cut(s) 408
Ecl136II GAGCTC 1 cut(s) 1541
Eco130I CCWWGG 3 cut(s) 231, 847, 1448
Eco147I AGGCCT 2 cut(s) 515, 1585
Eco24I GRGCYC 2 cut(s) 1198, 1543
Eco31I GGTCTC 2 cut(s) 105, 1326
Eco32I GATATC 1 cut(s) 1278
Eco47I GGWCC 8 cut(s) 27, 330, 465, 1006, 1098, 1430, 1476, 2016
Eco53kI GAGCTC 1 cut(s) 1541
Eco72I CACGTG 1 cut(s) 350
EcoICRI GAGCTC 1 cut(s) 1541
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoO109I RGGNCCY 2 cut(s) 976, 1476
EcoRII CCWGG 3 cut(s) 129, 335, 1463
EcoRV GATATC 1 cut(s) 1278
EcoT14I CCWWGG 3 cut(s) 231, 847, 1448
EcoT38I GRGCYC 2 cut(s) 1198, 1543
EgeI GGCGCC 1 cut(s) 250
EheI GGCGCC 1 cut(s) 250
ErhI CCWWGG 3 cut(s) 231, 847, 1448
Esp3I CGTCTC 1 cut(s) 1812
FaeI CATG 7 cut(s) 1105, 1276, 1337, 1636, 1655, 1691, 2116
FalI AAGNNNNNCTT 4 cut(s) 846, 878, 1995, 2027
FaqI GGGAC 7 cut(s) 392, 466, 719, 1141, 1242, 1416, 2205
FatI CATG 7 cut(s) 1101, 1272, 1333, 1632, 1651, 1687, 2112
FauI CCCGC 2 cut(s) 48, 369
FblI GTMKAC 2 cut(s) 159, 599
FokI GGATG 6 cut(s) 541, 801, 938, 1153, 1199, 1239
FriOI GRGCYC 2 cut(s) 1198, 1543
FspBI CTAG 2 cut(s) 702, 1589
GlaI GCGC 3 cut(s) 122, 250, 1112
GsuI CTGGAG 2 cut(s) 1131, 1494
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 7 cut(s) 424, 515, 978, 1463, 1585, 1770, 1865
HapII CCGG 7 cut(s) 12, 25, 115, 450, 519, 614, 1984
HgaI GACGC 1 cut(s) 610
HhaI GCGC 3 cut(s) 123, 251, 1113
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 7 cut(s) 1105, 1276, 1337, 1636, 1655, 1691, 2116
Hin6I GCGC 3 cut(s) 121, 249, 1111
HinP1I GCGC 3 cut(s) 121, 249, 1111
HincII GTYRAC 2 cut(s) 600, 1041
HindII GTYRAC 2 cut(s) 600, 1041
HindIII AAGCTT 3 cut(s) 1989, 2009, 2277
HinfI GANTC 7 cut(s) 89, 101, 197, 437, 1129, 1748, 2241
HpaI GTTAAC 1 cut(s) 1041
HpaII CCGG 7 cut(s) 12, 25, 115, 450, 519, 614, 1984
HphI GGTGA 6 cut(s) 398, 469, 616, 797, 1079, 1743
Hpy166II GTNNAC 6 cut(s) 76, 160, 600, 1009, 1041, 2233
Hpy8I GTNNAC 6 cut(s) 76, 160, 600, 1009, 1041, 2233
HpyAV CCTTC 8 cut(s) 667, 758, 967, 1453, 1587, 1655, 1782, 2228
HpyCH4IV ACGT 3 cut(s) 349, 410, 1118
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 297, 664, 1493, 1871, 2008, 2147
HpyF3I CTNAG 5 cut(s) 1224, 1389, 1902, 2337, 2375
HpySE526I ACGT 3 cut(s) 349, 410, 1118
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 7 cut(s) 1105, 1276, 1337, 1636, 1655, 1691, 2116
HspAI GCGC 3 cut(s) 121, 249, 1111
KasI GGCGCC 1 cut(s) 248
KspAI GTTAAC 1 cut(s) 1041
KspI CCGCGG 1 cut(s) 372
Kzo9I GATC 5 cut(s) 4, 306, 634, 1241, 2122
LguI GCTCTTC 1 cut(s) 539
Lsp1109I GCAGC 7 cut(s) 786, 814, 1094, 1397, 1623, 1639, 2039
LweI GCATC 8 cut(s) 388, 786, 916, 1261, 1287, 2137, 2164, 2202
MaeI CTAG 2 cut(s) 702, 1589
MaeII ACGT 3 cut(s) 349, 410, 1118
MaeIII GTNAC 9 cut(s) 345, 350, 386, 622, 916, 1067, 1371, 1603, 1855
MalI GATC 5 cut(s) 6, 308, 636, 1243, 2124
MbiI CCGCTC 1 cut(s) 376
MboI GATC 5 cut(s) 4, 306, 634, 1241, 2122
MfeI CAATTG 1 cut(s) 1212
MflI RGATCY 3 cut(s) 4, 634, 1241
MhlI GDGCHC 2 cut(s) 1198, 1543
Mly113I GGCGCC 1 cut(s) 249
MlyI GAGTC 5 cut(s) 110, 191, 446, 1123, 2235
MmeI TCCRAC 7 cut(s) 32, 129, 662, 1819, 1921, 2086, 2279
MroXI GAANNNNTTC 1 cut(s) 1155
MseI TTAA 7 cut(s) 710, 735, 908, 1020, 1040, 1734, 2264
MslI CAYNNNNRTG 1 cut(s) 819
MspA1I CMGCKG 4 cut(s) 371, 471, 1388, 2030
MspI CCGG 7 cut(s) 12, 25, 115, 450, 519, 614, 1984
MspR9I CCNGG 4 cut(s) 131, 337, 451, 1465
MunI CAATTG 1 cut(s) 1212
MvaI CCWGG 3 cut(s) 131, 337, 1465
MvnI CGCG 2 cut(s) 123, 371
MwoI GCNNNNNNNGC 7 cut(s) 21, 297, 664, 1493, 1871, 2008, 2147
NarI GGCGCC 1 cut(s) 249
NciI CCSGG 1 cut(s) 451
NdeII GATC 5 cut(s) 4, 306, 634, 1241, 2122
NlaIII CATG 7 cut(s) 1105, 1276, 1337, 1636, 1655, 1691, 2116
NmeAIII GCCGAG 2 cut(s) 389, 450
NmuCI GTSAC 6 cut(s) 345, 350, 386, 622, 1067, 1603
NspI RCATGY 3 cut(s) 1636, 1655, 2116
PaeI GCATGC 2 cut(s) 1655, 2116
PagI TCATGA 2 cut(s) 1272, 1333
PaqCI CACCTGC 1 cut(s) 1762
PceI AGGCCT 2 cut(s) 515, 1585
PciI ACATGT 1 cut(s) 1632
PciSI GCTCTTC 1 cut(s) 539
PcsI WCGNNNNNNNCGW 9 cut(s) 28, 78, 84, 153, 183, 186, 192, 276, 315
PdmI GAANNNNTTC 1 cut(s) 1155
PfeI GAWTC 2 cut(s) 89, 1748
Pfl23II CGTACG 1 cut(s) 144
PflFI GACNNNGTC 1 cut(s) 1102
PflMI CCANNNNNTGG 1 cut(s) 1940
PleI GAGTC 5 cut(s) 109, 191, 445, 1123, 2235
PluTI GGCGCC 1 cut(s) 252
PmaCI CACGTG 1 cut(s) 350
PmlI CACGTG 1 cut(s) 350
PpsI GAGTC 5 cut(s) 109, 191, 445, 1123, 2235
Ppu21I YACGTR 1 cut(s) 350
PpuMI RGGWCCY 1 cut(s) 1476
PscI ACATGT 1 cut(s) 1632
PsiI TTATAA 1 cut(s) 696
Psp124BI GAGCTC 1 cut(s) 1543
Psp5II RGGWCCY 1 cut(s) 1476
Psp6I CCWGG 3 cut(s) 129, 335, 1463
PspCI CACGTG 1 cut(s) 350
PspGI CCWGG 3 cut(s) 129, 335, 1463
PspLI CGTACG 1 cut(s) 144
PspPPI RGGWCCY 1 cut(s) 1476
PsrI GAACNNNNNNTAC 2 cut(s) 1356, 1388
PstI CTGCAG 2 cut(s) 804, 1387
PsuI RGATCY 3 cut(s) 4, 634, 1241
PsyI GACNNNGTC 1 cut(s) 1102
PvuII CAGCTG 2 cut(s) 1388, 2030
RsaI GTAC 7 cut(s) 146, 650, 833, 881, 1717, 1786, 1845
RsaNI GTAC 7 cut(s) 145, 649, 832, 880, 1716, 1785, 1844
RseI CAYNNNNRTG 1 cut(s) 819
SacI GAGCTC 1 cut(s) 1543
SacII CCGCGG 1 cut(s) 372
SalI GTCGAC 1 cut(s) 598
SapI GCTCTTC 1 cut(s) 539
SaqAI TTAA 7 cut(s) 710, 735, 908, 1020, 1040, 1734, 2264
Sau3AI GATC 5 cut(s) 4, 306, 634, 1241, 2122
ScaI AGTACT 1 cut(s) 881
SchI GAGTC 5 cut(s) 110, 191, 446, 1123, 2235
ScrFI CCNGG 4 cut(s) 131, 337, 451, 1465
SduI GDGCHC 2 cut(s) 1198, 1543
SfaNI GCATC 8 cut(s) 388, 786, 916, 1261, 1287, 2137, 2164, 2202
SfcI CTRYAG 4 cut(s) 800, 1383, 2142, 2153
SfoI GGCGCC 1 cut(s) 250
Sfr303I CCGCGG 1 cut(s) 372
SgrBI CCGCGG 1 cut(s) 372
SinI GGWCC 8 cut(s) 27, 330, 465, 1006, 1098, 1430, 1476, 2016
SmiMI CAYNNNNRTG 1 cut(s) 819
SmlI CTYRAG 4 cut(s) 753, 1283, 1301, 1912
SmoI CTYRAG 4 cut(s) 753, 1283, 1301, 1912
SphI GCATGC 2 cut(s) 1655, 2116
SseBI AGGCCT 2 cut(s) 515, 1585
SspDI GGCGCC 1 cut(s) 248
SspI AATATT 1 cut(s) 1033
SspMI CTAG 2 cut(s) 702, 1589
SstI GAGCTC 1 cut(s) 1543
StuI AGGCCT 2 cut(s) 515, 1585
StyD4I CCNGG 4 cut(s) 129, 335, 449, 1463
StyI CCWWGG 3 cut(s) 231, 847, 1448
TaiI ACGT 3 cut(s) 352, 413, 1121
TaqI TCGA 5 cut(s) 267, 407, 599, 1413, 2389
TaqII GACCGA 1 cut(s) 123
TatI WGTACW 1 cut(s) 879
TfiI GAWTC 2 cut(s) 89, 1748
Tru1I TTAA 7 cut(s) 710, 735, 908, 1020, 1040, 1734, 2264
Tru9I TTAA 7 cut(s) 710, 735, 908, 1020, 1040, 1734, 2264
TscAI CASTG 5 cut(s) 556, 1783, 1968, 2004, 2046
TseFI GTSAC 6 cut(s) 345, 350, 386, 622, 1067, 1603
TseI GCWGC 7 cut(s) 799, 802, 1082, 1385, 1611, 1627, 2027
Tsp45I GTSAC 6 cut(s) 345, 350, 386, 622, 1067, 1603
TspRI CASTG 5 cut(s) 556, 1783, 1968, 2004, 2046
Tth111I GACNNNGTC 1 cut(s) 1102
Van91I CCANNNNNTGG 1 cut(s) 1940
VpaK11BI GGWCC 8 cut(s) 27, 330, 465, 1006, 1098, 1430, 1476, 2016
XagI CCTNNNNNAGG 1 cut(s) 230
XapI RAATTY 2 cut(s) 1406, 1728
XceI RCATGY 3 cut(s) 1636, 1655, 2116
XmiI GTMKAC 2 cut(s) 159, 599
XmnI GAANNNNTTC 1 cut(s) 1155
XspI CTAG 2 cut(s) 702, 1589
ZrmI AGTACT 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.