Rorug04G0306400

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009465
Physical Location & Seq
Reverse (-)
48268412 .. 48269201
790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0306400.1

Sequence Viewer

Length: 711 bp
ATGAACGAGAAGGCCAACGTCACCATGGAGCTTAACGCCAAGCACAGAAAGATACTGGAAGGACTTGTTAAATTGCCGGAGAATAGGGAATGTGCTGACTGCAAAGCCAAGTGTCCAAGATGGGCCAGTGTGAACTTAGGCATCTTTATATGCATGCAGTGTTCTGGGATCCACAGAAGTCTTGGGGTACACATATCCAAGGTGAGGTCTGCTACCTTGGACACATGGCTTCCAGAGCAAGTTGCATTTATTCAATCAATGGGAAATGAAAAGGCAAATAATAAGAGATGGGCCGCTAAAGATGGAAACCCAAAATCACCTTGTAGAGGACGCGAGCAAACTGCTTGCAAACATTGGCAGGGACCTGTGGAAAGCAGTGGGCATGGGTATATAGGAAACCCAGAAAAGAAATTGGAGGAAAGGAAGAACATACAAACAGCTAGTAGAAGAGAAACTGTTCCTGCTGGAAGAATCAGTCTTCCTGTTCCTCCTGAACATAAACCACAAAATGTTCAGAAAATAGAACCAGCAATTCCACAGGCTGAAGCAATAAAGCCAGCTGCTGAAGTTGCCCCTCCAAAGATTGATTTTGCTACTGACCTTATCGGCATGCTCTCAGTCGTAGATAGTCTCAGTGAGAATAGCTCATCAGATGCAGCACCAGCTGACGATAATGATTGGGCAGGTTTCCAGTCGGCTAACGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006950 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007266 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0009893 GO:0009966 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0012501 GO:0016043 GO:0016301 GO:0016310 GO:0016477 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017048 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023051 GO:0023052 GO:0030029 GO:0030036 GO:0031098 GO:0031267 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0040011 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043408 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048365 GO:0048518 GO:0048522 GO:0048583 GO:0048870 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051674 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902531
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

236

Amino Acids

25.82

Weight (kDa)

8.14

Isoelectric Point (pI)

55.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 17 - 113 2e-35 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 674
AccII CGCG 1 cut(s) 333
AciI CCGC 1 cut(s) 294
AclWI GGATC 2 cut(s) 163, 176
AcuI CTGAAG 2 cut(s) 564, 585
AfaI GTAC 1 cut(s) 189
AfiI CCNNNNNNNGG 2 cut(s) 204, 326
AgsI TTSAA 1 cut(s) 254
AluBI AGCT 5 cut(s) 31, 440, 560, 645, 665
AluI AGCT 5 cut(s) 31, 440, 560, 645, 665
Alw26I GTCTC 1 cut(s) 635
AlwI GGATC 2 cut(s) 163, 176
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 3 cut(s) 12, 123, 291
ApeKI GCWGC 2 cut(s) 560, 656
Asp700I GAANNNNTTC 1 cut(s) 456
AspS9I GGNCC 3 cut(s) 123, 291, 362
AsuHPI GGTGA 3 cut(s) 13, 214, 309
AvaII GGWCC 1 cut(s) 362
BamHI GGATCC 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 470
BbvI GCAGC 2 cut(s) 547, 668
BccI CCATC 3 cut(s) 114, 282, 296
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BcoDI GTCTC 1 cut(s) 635
BfaI CTAG 1 cut(s) 441
BfuAI ACCTGC 1 cut(s) 674
BisI GCNGC 3 cut(s) 294, 561, 657
BlsI GCNGC 3 cut(s) 295, 562, 658
Bme18I GGWCC 1 cut(s) 362
BmgT120I GGNCC 3 cut(s) 123, 291, 362
BmiI GGNNCC 2 cut(s) 170, 363
BmsI GCATC 2 cut(s) 150, 643
BpiI GAAGAC 1 cut(s) 470
BplI GAGNNNNNCTC 2 cut(s) 629, 661
BsaJI CCNNGG 3 cut(s) 24, 198, 216
Bsc4I CCNNNNNNNGG 2 cut(s) 204, 326
Bse1I ACTGG 3 cut(s) 60, 126, 691
BseDI CCNNGG 3 cut(s) 24, 198, 216
BseLI CCNNNNNNNGG 2 cut(s) 204, 326
BseMII CTCAG 2 cut(s) 630, 646
BseNI ACTGG 3 cut(s) 60, 126, 691
BseXI GCAGC 2 cut(s) 547, 668
Bsh1236I CGCG 1 cut(s) 333
BshFI GGCC 3 cut(s) 14, 125, 293
BsiSI CCGG 1 cut(s) 77
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 2 cut(s) 204, 326
BsmAI GTCTC 1 cut(s) 635
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 3 cut(s) 14, 125, 293
Bsp143I GATC 1 cut(s) 168
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 1 cut(s) 294
BspANI GGCC 3 cut(s) 14, 125, 293
BspCNI CTCAG 2 cut(s) 629, 645
BspFNI CGCG 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 170, 363
BspMI ACCTGC 1 cut(s) 674
BspPI GGATC 2 cut(s) 163, 176
BsrI ACTGG 3 cut(s) 60, 126, 691
BssECI CCNNGG 3 cut(s) 24, 198, 216
BssMI GATC 1 cut(s) 168
BssT1I CCWWGG 3 cut(s) 24, 198, 216
Bst4CI ACNGT 1 cut(s) 457
Bst6I CTCTTC 1 cut(s) 442
BstC8I GCNNGC 5 cut(s) 155, 335, 346, 558, 611
BstDEI CTNAG 3 cut(s) 136, 616, 632
BstDSI CCRYGG 1 cut(s) 24
BstENI CCTNNNNNAGG 1 cut(s) 324
BstFNI CGCG 1 cut(s) 333
BstKTI GATC 1 cut(s) 171
BstMAI GTCTC 1 cut(s) 635
BstMBI GATC 1 cut(s) 168
BstMWI GCNNNNNNNGC 4 cut(s) 235, 569, 662, 704
BstNSI RCATGY 2 cut(s) 157, 613
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 547, 668
BstV2I GAAGAC 1 cut(s) 470
BstX2I RGATCY 1 cut(s) 168
BstYI RGATCY 1 cut(s) 168
BsuRI GGCC 3 cut(s) 14, 125, 293
BtgI CCRYGG 1 cut(s) 24
BtsI GCAGTG 2 cut(s) 164, 382
BtsIMutI CAGTG 4 cut(s) 133, 164, 382, 640
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 5 cut(s) 155, 335, 346, 558, 611
CaiI CAGNNNCTG 1 cut(s) 563
Cfr13I GGNCC 3 cut(s) 123, 291, 362
CseI GACGC 1 cut(s) 339
Csp6I GTAC 1 cut(s) 188
CviAII CATG 5 cut(s) 25, 154, 225, 383, 610
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 3 cut(s) 136, 616, 632
DpnI GATC 1 cut(s) 170
DpnII GATC 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 442
EarI CTCTTC 1 cut(s) 442
Eco130I CCWWGG 3 cut(s) 24, 198, 216
Eco47I GGWCC 1 cut(s) 362
Eco57I CTGAAG 2 cut(s) 564, 585
EcoNI CCTNNNNNAGG 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 362
EcoT14I CCWWGG 3 cut(s) 24, 198, 216
EcoT22I ATGCAT 1 cut(s) 155
ErhI CCWWGG 3 cut(s) 24, 198, 216
FaeI CATG 5 cut(s) 28, 157, 228, 386, 613
FaqI GGGAC 1 cut(s) 375
FatI CATG 5 cut(s) 24, 153, 224, 382, 609
Fnu4HI GCNGC 3 cut(s) 294, 561, 657
Fsp4HI GCNGC 3 cut(s) 294, 561, 657
FspBI CTAG 1 cut(s) 441
GluI GCNGC 3 cut(s) 294, 561, 657
HaeIII GGCC 3 cut(s) 14, 125, 293
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 339
Hin1II CATG 5 cut(s) 28, 157, 228, 386, 613
HinfI GANTC 1 cut(s) 471
HpaII CCGG 1 cut(s) 77
HphI GGTGA 3 cut(s) 13, 214, 309
Hpy166II GTNNAC 2 cut(s) 133, 190
Hpy188I TCNGA 2 cut(s) 516, 652
Hpy188III TCNNGA 2 cut(s) 233, 491
Hpy8I GTNNAC 2 cut(s) 133, 190
HpyAV CCTTC 2 cut(s) 4, 53
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 6 cut(s) 102, 153, 157, 245, 348, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 235, 569, 662, 704
HpyF3I CTNAG 3 cut(s) 136, 616, 632
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 5 cut(s) 28, 157, 228, 386, 613
Kzo9I GATC 1 cut(s) 168
LmnI GCTCC 1 cut(s) 28
Lsp1109I GCAGC 2 cut(s) 547, 668
LweI GCATC 2 cut(s) 150, 643
MaeI CTAG 1 cut(s) 441
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 19
MalI GATC 1 cut(s) 170
MboI GATC 1 cut(s) 168
MboII GAAGA 4 cut(s) 436, 459, 470, 480
MflI RGATCY 1 cut(s) 168
MluCI AATT 3 cut(s) 71, 410, 531
MnlI CCTC 5 cut(s) 198, 320, 409, 498, 585
Mph1103I ATGCAT 1 cut(s) 155
MroXI GAANNNNTTC 1 cut(s) 456
MseI TTAA 2 cut(s) 33, 69
MspA1I CMGCKG 2 cut(s) 560, 665
MspI CCGG 1 cut(s) 77
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 4 cut(s) 235, 569, 662, 704
NcoI CCATGG 1 cut(s) 24
NdeII GATC 1 cut(s) 168
NlaIII CATG 5 cut(s) 28, 157, 228, 386, 613
NlaIV GGNNCC 2 cut(s) 170, 363
NmuCI GTSAC 1 cut(s) 19
NsiI ATGCAT 1 cut(s) 155
NspI RCATGY 2 cut(s) 157, 613
PaeI GCATGC 2 cut(s) 157, 613
PdmI GAANNNNTTC 1 cut(s) 456
PfeI GAWTC 1 cut(s) 471
PkrI GCNGC 3 cut(s) 295, 562, 658
PpuMI RGGWCCY 1 cut(s) 362
Psp5II RGGWCCY 1 cut(s) 362
PspN4I GGNNCC 2 cut(s) 170, 363
PspPI GGNCC 3 cut(s) 123, 291, 362
PspPPI RGGWCCY 1 cut(s) 362
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 1 cut(s) 168
PvuII CAGCTG 2 cut(s) 560, 665
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
SaqAI TTAA 2 cut(s) 33, 69
SatI GCNGC 3 cut(s) 294, 561, 657
Sau3AI GATC 1 cut(s) 168
Sau96I GGNCC 3 cut(s) 123, 291, 362
SfaNI GCATC 2 cut(s) 150, 643
SinI GGWCC 1 cut(s) 362
SphI GCATGC 2 cut(s) 157, 613
Sse9I AATT 3 cut(s) 71, 410, 531
SsiI CCGC 1 cut(s) 294
SspMI CTAG 1 cut(s) 441
StyI CCWWGG 3 cut(s) 24, 198, 216
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 1 cut(s) 21
TasI AATT 3 cut(s) 71, 410, 531
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 1 cut(s) 471
Tru1I TTAA 2 cut(s) 33, 69
Tru9I TTAA 2 cut(s) 33, 69
TscAI CASTG 4 cut(s) 133, 164, 382, 640
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 2 cut(s) 560, 656
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 17, 282
TspRI CASTG 4 cut(s) 133, 164, 382, 640
VpaK11BI GGWCC 1 cut(s) 362
XagI CCTNNNNNAGG 1 cut(s) 324
XceI RCATGY 2 cut(s) 157, 613
XcmI CCANNNNNNNNNTGG 2 cut(s) 22, 179
XmnI GAANNNNTTC 1 cut(s) 456
XspI CTAG 1 cut(s) 441
Zsp2I ATGCAT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.