MD13G1060400.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009465
Physical Location & Seq
Reverse (-)
4240083 .. 4246954
6872 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1060400.v1.1.491

Sequence Viewer

Length: 2400 bp
ATGGATCCGACCCCCACCACCCGCCGGACCCGGAAAACTCCACCCAAACCCGATTACTCAACCGTAGTCATCCATGACGACGATGGCTCCAACTCAGAATCCGAACGTCGGAGCAAACCCAAGAGCTCCGACCCGGAAACCGACCTCTACGCCACAATGGTCTACAAGGGTAATGCCGACGAAGACGAAGACGAAGATGACGACGCCTCTCTTCCTCCGCTCCTCAAACGCCTCCCCAAGGACTTCGGCGGCGGAGCTCCCATCGATTACTTCGATGACGAGGAGGAAGACGAAAATGGCGGCGATTTCGGCACAATGATCGTCAAGCCCGATCGGAATCGGGCCACGAGGAGGTCGCGCGACTTCAAGCGGGCGTCTTTCGACGATGGCGGAGACGGCGATGGGTTCTCGACCTTTGTGGTGAGGCCGTCGAGTGAGAGAGAGTCTGTTAGCGGGACCGTGGTGAGGAGGACAAGCAGCGGCGTTGGTTCGACGATGAGTAGGGCAGTGGCAAGCATGCAGGCCTCGTCGGAGTCAGGCTTTGGGAAGCAGAGGAGGGGCAGTGGGTCGTCGCAGGGCGAAGAGTATCGCCAAACCACTAAAATGTCTTCGAGTTCGATACCCGATAGCATTACCAGAGAAGACCCTACTGTGAAGTACGACCTGCTCAATGAGCTTGGGAAGGGGTCTTATGGCGCTGTTTATAAAGCTAGGGATATTAAGACGTCGGAGCTGGTGGCGATCAAAGTCATTTCATTGTCTCAAGGGGAGGAGGGTTATGAAGAAATTTGCGGTGAAATTGAGATGCTGCAACAATGTAATCATCCGAATGTGGTTCGGTATCTGGGGAGCTACCAAGGAGAAGAGTATCTTTGGATTGTGATGGAGTACTGTGGTGGTGGCAGTGTTGCCGACTTGATGAATGTTACCGAGGATGCTCTGGAGGAGTATCAAATAGCCTTCATTTGTAGGGAGGCGTTGAAGGGCCTTGCTTATCTGCACTCGATTTTCAAGGTCCACAGAGATATTAAGGGTGGTAATATTCTGTTAACTGAGCAAGGAGATGTCAAGTTGGGTGATTTTGGTGTTGCAGCTCAACTTACAAGAACCATGTCAAAGCGCAACACGTTCATTGGCACTCCACATTGGATGGCTCCAGAAGTTATTCAGGAAAGTCGTTATGATGGAAAGGTGGATGTATGGGCTCTTGGTGTTTCTGCAATTGAAATGGCAGAGGGCCTCCCTCCAAGATCTTCGGTGCATCCAATGAGGGTTTTATTCATGATATCCCTTGAGCCAGCCCCGATGCTTGAGGATAAAGAAAAATGGTCTCTTGTGTTTCATGATTTTGTGGCAAAGTCCCTAACTAAAGAACCTCGTCTACGCCCTACTGCATCTGAGATGCTGAAGCACAAGTTCATTGAAAAATGCAAATGTGGTCCCTCTGCAATGTTGGCAAAGATTGAAAAGGCAAGGCAAGCAAGGGCTTCAATGGCTTTGCAAGCACAAAATATTGCTCCACCTGAACGGGACAATACAATTGAAGCTCCCAAAGTCAATGAGGATTATGGAGATACTGTCCCTTCAAGGCCACTTCAAGGAGAAAATGAAGTATCTTCAGGTAGCACATTGAGGAAGCAGCACATATCTGGTGATGTGGGAATGGCTGGGGAAGGTAACTTTGGCACTTTTATTATTCATGAGGGGGATGAAAGAGATGAGATCTCTAGCGAGACAGAGAGTTTTAGTGCCAAAGAACCTTCACCTGCTCCTGGATTTCCTGAAAGCTCTTCCAATACTGGCGCAGGAGGCAAATTGCCCGAACCCAGGGAGGAAAATTCAAGTGGTATTACCCAAAACAGCAGTCCAGTTGCGCACACTCTACGGGCATCTGAGCCGAGCCTAAAGACAAAGGGCATATCTCAAGTCCAGGTTGGAGGCCCAAGCAGTATAAGCAGTGGCACGGTAAAGAATGAAACTGTCAGCCGGAAAGCTTTTGCAATGCAAGATAAGCTTTGGTCCATATATGCAGCTGGTAATACAGTGCCTATACCATTTTTGAGGGCAACCGATATATCCCCCATTGCTCTCTTATCAGACAATGTTCTTGGAGGCATGCATCAAGATAACAGTGGAACTGTAGCCATGGAGGCACTACAGGAGCTTTTTACTGGTGATGGACAGTCTAAGAAGGGACGAAGAGGGCAAAACGAGATGCCCCTCCCTCCAAGTGTTTACAAAAGGCTCTTTACAAGTCCAACTCTAATGAATCTGGCACAAGCATTAGCCTACCACAAGATGTGCTACGAAGATATGCCGCTCCAAGAAATGCAAGCAACTCAGGAACAACAGACTATTCAAAATCTTTGTGATACACTAAGAACCATTCTTAGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006950 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007266 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0009893 GO:0009966 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0012501 GO:0016043 GO:0016301 GO:0016310 GO:0016477 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017048 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023051 GO:0023052 GO:0030029 GO:0030036 GO:0031098 GO:0031267 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0040011 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043408 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048365 GO:0048518 GO:0048522 GO:0048583 GO:0048870 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051674 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902531
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

800

Amino Acids

87.63

Weight (kDa)

5.16

Isoelectric Point (pI)

57.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 220 - 474 5.3e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 222 - 470 6.5e-48 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 293 - 371 3.7e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
AarI CACCTGC 1 cut(s) 1774
AatII GACGTC 1 cut(s) 728
Acc16I TGCGCA 1 cut(s) 1875
Acc36I ACCTGC 2 cut(s) 672, 1774
AccBSI CCGCTC 2 cut(s) 220, 2320
AccI GTMKAC 2 cut(s) 162, 1381
AccII CGCG 2 cut(s) 358, 360
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 786, 1837
AcuI CTGAAG 2 cut(s) 1427, 1602
AcyI GRCGYC 3 cut(s) 204, 374, 725
AfaI GTAC 2 cut(s) 659, 890
AfiI CCNNNNNNNGG 8 cut(s) 24, 108, 238, 351, 465, 1598, 1772, 1827
AflIII ACRYGT 1 cut(s) 1125
AjnI CCWGG 3 cut(s) 1771, 1826, 1929
AjuI GAANNNNNNNTTGG 2 cut(s) 1665, 1697
Alw21I GWGCWC 2 cut(s) 128, 259
Alw26I GTCTC 4 cut(s) 387, 765, 1335, 1727
AlwI GGATC 1 cut(s) 12
AoxI GGCC 7 cut(s) 342, 425, 522, 985, 1237, 1589, 1939
ApeKI GCWGC 5 cut(s) 477, 808, 1091, 1639, 2030
ApoI RAATTY 2 cut(s) 786, 1837
ArsI GACNNNNNNTTYG 2 cut(s) 1849, 1881
Asp700I GAANNNNTTC 1 cut(s) 1164
AspLEI GCGC 5 cut(s) 360, 698, 1122, 1805, 1876
AspS9I GGNCC 9 cut(s) 27, 342, 456, 985, 1015, 1237, 1439, 1940, 2019
AsuC2I CCSGG 2 cut(s) 31, 134
AsuHPI GGTGA 7 cut(s) 433, 475, 806, 1088, 1664, 1755, 2186
AvaII GGWCC 5 cut(s) 27, 456, 1015, 1439, 2019
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 3 cut(s) 128, 259, 1207
BauI CACGAG 1 cut(s) 346
BbsI GAAGAC 5 cut(s) 189, 195, 294, 600, 648
Bbv12I GWGCWC 2 cut(s) 128, 259
BbvI GCAGC 5 cut(s) 489, 795, 1103, 1651, 2042
BccI CCATC 8 cut(s) 77, 269, 380, 395, 877, 1144, 1178, 2171
BceAI ACGGC 2 cut(s) 412, 412
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciT130I CCWGG 3 cut(s) 1773, 1828, 1931
BcnI CCSGG 2 cut(s) 31, 134
BcoDI GTCTC 4 cut(s) 387, 765, 1335, 1727
BfaI CTAG 2 cut(s) 711, 1728
BfmI CTRYAG 2 cut(s) 2139, 2156
BfoI RGCGCY 1 cut(s) 699
BfuAI ACCTGC 2 cut(s) 672, 1774
BglI GCCNNNNNGGC 1 cut(s) 2150
BglII AGATCT 2 cut(s) 1250, 1722
BisI GCNGC 9 cut(s) 250, 301, 478, 481, 809, 1092, 1640, 2031, 2318
BlsI GCNGC 9 cut(s) 251, 302, 479, 482, 810, 1093, 1641, 2032, 2319
BmcAI AGTACT 1 cut(s) 890
Bme1390I CCNGG 5 cut(s) 31, 134, 1773, 1828, 1931
Bme18I GGWCC 5 cut(s) 27, 456, 1015, 1439, 2019
BmgT120I GGNCC 9 cut(s) 27, 342, 456, 985, 1015, 1237, 1439, 1940, 2019
BmiI GGNNCC 6 cut(s) 6, 29, 88, 457, 1155, 1441
BmrFI CCNGG 5 cut(s) 31, 134, 1773, 1828, 1931
BmsI GCATC 9 cut(s) 795, 925, 1270, 1296, 1392, 1403, 1898, 2128, 2205
BpiI GAAGAC 5 cut(s) 189, 195, 294, 600, 648
BpmI CTGGAG 2 cut(s) 962, 1140
BpuEI CTTGAG 4 cut(s) 747, 1313, 1331, 1908
BpuMI CCSGG 2 cut(s) 31, 134
Bsa29I ATCGAT 1 cut(s) 264
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 3 cut(s) 204, 374, 725
BsaI GGTCTC 1 cut(s) 1335
BsaJI CCNNGG 7 cut(s) 237, 459, 856, 930, 1826, 1827, 2145
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 547, 577, 1563, 1593
Bsc4I CCNNNNNNNGG 8 cut(s) 24, 108, 238, 351, 465, 1598, 1772, 1827
Bse1I ACTGG 3 cut(s) 1804, 1868, 2176
Bse3DI GCAATG 3 cut(s) 1455, 2007, 2082
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 3 cut(s) 1773, 1828, 1931
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 7 cut(s) 237, 459, 856, 930, 1826, 1827, 2145
BseGI GGATG 7 cut(s) 69, 823, 940, 1155, 1201, 1261, 1714
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 8 cut(s) 24, 108, 238, 351, 465, 1598, 1772, 1827
BseMI GCAATG 3 cut(s) 1455, 2007, 2082
BseMII CTCAG 5 cut(s) 108, 1044, 1389, 1884, 2354
BseNI ACTGG 3 cut(s) 1804, 1868, 2176
BseRI GAGGAG 7 cut(s) 212, 296, 364, 481, 568, 785, 959
BseXI GCAGC 5 cut(s) 489, 795, 1103, 1651, 2042
BseYI CCCAGC 1 cut(s) 1667
BsgI GTGCAG 1 cut(s) 983
Bsh1236I CGCG 2 cut(s) 358, 360
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 7 cut(s) 344, 427, 524, 987, 1239, 1591, 1941
BshVI ATCGAT 1 cut(s) 264
BsiEI CGRYCG 1 cut(s) 334
BsiHKAI GWGCWC 2 cut(s) 128, 259
BsiSI CCGG 4 cut(s) 25, 31, 134, 1987
BslFI GGGAC 6 cut(s) 469, 1345, 1425, 1544, 1565, 2208
BslI CCNNNNNNNGG 8 cut(s) 24, 108, 238, 351, 465, 1598, 1772, 1827
BsmAI GTCTC 4 cut(s) 387, 765, 1335, 1727
BsmBI CGTCTC 1 cut(s) 387
BsmFI GGGAC 6 cut(s) 469, 1345, 1425, 1544, 1565, 2208
BsnI GGCC 7 cut(s) 344, 427, 524, 987, 1239, 1591, 1941
Bso31I GGTCTC 1 cut(s) 1335
Bsp1286I GDGCHC 3 cut(s) 128, 259, 1207
Bsp143I GATC 6 cut(s) 4, 318, 331, 741, 1250, 1722
Bsp19I CCATGG 1 cut(s) 2145
BspANI GGCC 7 cut(s) 344, 427, 524, 987, 1239, 1591, 1941
BspCNI CTCAG 5 cut(s) 107, 1045, 1390, 1885, 2353
BspDI ATCGAT 1 cut(s) 264
BspFNI CGCG 2 cut(s) 358, 360
BspHI TCATGA 3 cut(s) 1281, 1342, 1699
BspLI GGNNCC 6 cut(s) 6, 29, 88, 457, 1155, 1441
BspMI ACCTGC 2 cut(s) 672, 1774
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 1795
BspTNI GGTCTC 1 cut(s) 1335
BsrBI CCGCTC 2 cut(s) 220, 2320
BsrDI GCAATG 3 cut(s) 1455, 2007, 2082
BsrI ACTGG 3 cut(s) 1804, 1868, 2176
BssECI CCNNGG 7 cut(s) 237, 459, 856, 930, 1826, 1827, 2145
BssMI GATC 6 cut(s) 4, 318, 331, 741, 1250, 1722
BssNI GRCGYC 3 cut(s) 204, 374, 725
BssSI CACGAG 1 cut(s) 346
BssT1I CCWWGG 3 cut(s) 237, 856, 2145
Bst2BI CACGAG 1 cut(s) 346
Bst2UI CCWGG 3 cut(s) 1773, 1828, 1931
Bst6I CTCTTC 5 cut(s) 216, 576, 858, 1795, 2194
BstACI GRCGYC 3 cut(s) 204, 374, 725
BstC8I GCNNGC 9 cut(s) 372, 514, 518, 522, 1299, 1479, 1503, 2117, 2334
BstDEI CTNAG 8 cut(s) 94, 1053, 1398, 1893, 2187, 2340, 2378, 2390
BstDSI CCRYGG 2 cut(s) 459, 2145
BstENI CCTNNNNNAGG 1 cut(s) 236
BstF5I GGATG 7 cut(s) 69, 823, 940, 1155, 1201, 1261, 1714
BstFNI CGCG 2 cut(s) 358, 360
BstH2I RGCGCY 1 cut(s) 699
BstHHI GCGC 5 cut(s) 360, 698, 1122, 1805, 1876
BstKTI GATC 6 cut(s) 7, 321, 334, 744, 1253, 1725
BstMAI GTCTC 4 cut(s) 387, 765, 1335, 1727
BstMBI GATC 6 cut(s) 4, 318, 331, 741, 1250, 1722
BstMCI CGRYCG 1 cut(s) 334
BstNI CCWGG 3 cut(s) 1773, 1828, 1931
BstNSI RCATGY 2 cut(s) 520, 2119
BstSCI CCNGG 5 cut(s) 29, 132, 1771, 1826, 1929
BstSFI CTRYAG 2 cut(s) 2139, 2156
BstUI CGCG 2 cut(s) 358, 360
BstV1I GCAGC 5 cut(s) 489, 795, 1103, 1651, 2042
BstV2I GAAGAC 5 cut(s) 189, 195, 294, 600, 648
BstX2I RGATCY 3 cut(s) 4, 1250, 1722
BstYI RGATCY 3 cut(s) 4, 1250, 1722
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 7 cut(s) 344, 427, 524, 987, 1239, 1591, 1941
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 2 cut(s) 459, 2145
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 7 cut(s) 69, 823, 940, 1155, 1201, 1261, 1714
BtsI GCAGTG 4 cut(s) 513, 568, 910, 1963
BtsIMutI CAGTG 6 cut(s) 513, 568, 910, 1963, 2049, 2137
BveI ACCTGC 2 cut(s) 672, 1774
Cac8I GCNNGC 9 cut(s) 372, 514, 518, 522, 1299, 1479, 1503, 2117, 2334
CciI TCATGA 3 cut(s) 1281, 1342, 1699
CfoI GCGC 5 cut(s) 360, 698, 1122, 1805, 1876
Cfr13I GGNCC 9 cut(s) 27, 342, 456, 985, 1015, 1237, 1439, 1940, 2019
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 2 cut(s) 212, 363
Csp6I GTAC 2 cut(s) 658, 889
CviAII CATG 8 cut(s) 74, 517, 1111, 1282, 1343, 1700, 2116, 2146
CviQI GTAC 2 cut(s) 658, 889
DdeI CTNAG 8 cut(s) 94, 1053, 1398, 1893, 2187, 2340, 2378, 2390
DpnI GATC 6 cut(s) 6, 320, 333, 743, 1252, 1724
DpnII GATC 6 cut(s) 4, 318, 331, 741, 1250, 1722
Eam1104I CTCTTC 5 cut(s) 216, 576, 858, 1795, 2194
EarI CTCTTC 5 cut(s) 216, 576, 858, 1795, 2194
EciI GGCGGA 2 cut(s) 267, 405
Ecl136II GAGCTC 2 cut(s) 126, 257
Eco130I CCWWGG 3 cut(s) 237, 856, 2145
Eco147I AGGCCT 1 cut(s) 524
Eco24I GRGCYC 3 cut(s) 128, 259, 1207
Eco31I GGTCTC 1 cut(s) 1335
Eco32I GATATC 1 cut(s) 1287
Eco47I GGWCC 5 cut(s) 27, 456, 1015, 1439, 2019
Eco53kI GAGCTC 2 cut(s) 126, 257
Eco57I CTGAAG 2 cut(s) 1427, 1602
EcoICRI GAGCTC 2 cut(s) 126, 257
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoO109I RGGNCCY 2 cut(s) 985, 1237
EcoRII CCWGG 3 cut(s) 1771, 1826, 1929
EcoRV GATATC 1 cut(s) 1287
EcoT14I CCWWGG 3 cut(s) 237, 856, 2145
EcoT22I ATGCAT 1 cut(s) 2121
EcoT38I GRGCYC 3 cut(s) 128, 259, 1207
ErhI CCWWGG 3 cut(s) 237, 856, 2145
Esp3I CGTCTC 1 cut(s) 387
FaeI CATG 8 cut(s) 77, 520, 1114, 1285, 1346, 1703, 2119, 2149
FalI AAGNNNNNCTT 4 cut(s) 855, 887, 1998, 2030
FaqI GGGAC 6 cut(s) 469, 1345, 1425, 1544, 1565, 2208
FatI CATG 8 cut(s) 73, 516, 1110, 1281, 1342, 1699, 2115, 2145
FauI CCCGC 3 cut(s) 29, 363, 446
FblI GTMKAC 2 cut(s) 162, 1381
Fnu4HI GCNGC 9 cut(s) 250, 301, 478, 481, 809, 1092, 1640, 2031, 2318
FokI GGATG 7 cut(s) 56, 810, 947, 1162, 1208, 1248, 1721
FriOI GRGCYC 3 cut(s) 128, 259, 1207
Fsp4HI GCNGC 9 cut(s) 250, 301, 478, 481, 809, 1092, 1640, 2031, 2318
FspBI CTAG 2 cut(s) 711, 1728
FspI TGCGCA 1 cut(s) 1875
GlaI GCGC 5 cut(s) 359, 697, 1121, 1804, 1875
GluI GCNGC 9 cut(s) 250, 301, 478, 481, 809, 1092, 1640, 2031, 2318
GsaI CCCAGC 1 cut(s) 1671
GsuI CTGGAG 2 cut(s) 962, 1140
HaeII RGCGCY 1 cut(s) 699
HaeIII GGCC 7 cut(s) 344, 427, 524, 987, 1239, 1591, 1941
HapII CCGG 4 cut(s) 25, 31, 134, 1987
HgaI GACGC 2 cut(s) 212, 363
HhaI GCGC 5 cut(s) 360, 698, 1122, 1805, 1876
Hin1I GRCGYC 3 cut(s) 204, 374, 725
Hin1II CATG 8 cut(s) 77, 520, 1114, 1285, 1346, 1703, 2119, 2149
Hin6I GCGC 5 cut(s) 358, 696, 1120, 1803, 1874
HinP1I GCGC 5 cut(s) 358, 696, 1120, 1803, 1874
HincII GTYRAC 1 cut(s) 1050
HindII GTYRAC 1 cut(s) 1050
HindIII AAGCTT 2 cut(s) 1992, 2012
HinfI GANTC 5 cut(s) 98, 337, 443, 533, 2269
HpaI GTTAAC 1 cut(s) 1050
HpaII CCGG 4 cut(s) 25, 31, 134, 1987
HphI GGTGA 7 cut(s) 433, 475, 806, 1088, 1664, 1755, 2186
Hpy166II GTNNAC 5 cut(s) 163, 1018, 1050, 1382, 2236
Hpy8I GTNNAC 5 cut(s) 163, 1018, 1050, 1382, 2236
HpyAV CCTTC 7 cut(s) 676, 970, 976, 1593, 1667, 1770, 2185
HpyCH4IV ACGT 3 cut(s) 106, 725, 1127
HpyF3I CTNAG 8 cut(s) 94, 1053, 1398, 1893, 2187, 2340, 2378, 2390
HpySE526I ACGT 3 cut(s) 106, 725, 1127
Hsp92I GRCGYC 3 cut(s) 204, 374, 725
Hsp92II CATG 8 cut(s) 77, 520, 1114, 1285, 1346, 1703, 2119, 2149
HspAI GCGC 5 cut(s) 358, 696, 1120, 1803, 1874
KspAI GTTAAC 1 cut(s) 1050
Kzo9I GATC 6 cut(s) 4, 318, 331, 741, 1250, 1722
LguI GCTCTTC 1 cut(s) 1795
Lsp1109I GCAGC 5 cut(s) 489, 795, 1103, 1651, 2042
LweI GCATC 9 cut(s) 795, 925, 1270, 1296, 1392, 1403, 1898, 2128, 2205
MaeI CTAG 2 cut(s) 711, 1728
MaeII ACGT 3 cut(s) 106, 725, 1127
MaeIII GTNAC 2 cut(s) 925, 1676
MalI GATC 6 cut(s) 6, 320, 333, 743, 1252, 1724
MbiI CCGCTC 2 cut(s) 220, 2320
MboI GATC 6 cut(s) 4, 318, 331, 741, 1250, 1722
MfeI CAATTG 2 cut(s) 1221, 1539
MflI RGATCY 3 cut(s) 4, 1250, 1722
MhlI GDGCHC 3 cut(s) 128, 259, 1207
MluCI AATT 6 cut(s) 786, 798, 1221, 1539, 1814, 1837
MlyI GAGTC 2 cut(s) 452, 542
MmeI TCCRAC 8 cut(s) 32, 89, 114, 153, 510, 708, 1915, 2282
Mph1103I ATGCAT 1 cut(s) 2121
MroXI GAANNNNTTC 1 cut(s) 1164
MseI TTAA 3 cut(s) 720, 1029, 1049
MslI CAYNNNNRTG 2 cut(s) 602, 828
MspA1I CMGCKG 2 cut(s) 480, 2033
MspI CCGG 4 cut(s) 25, 31, 134, 1987
MspR9I CCNGG 5 cut(s) 31, 134, 1773, 1828, 1931
MunI CAATTG 2 cut(s) 1221, 1539
MvaI CCWGG 3 cut(s) 1773, 1828, 1931
MvnI CGCG 2 cut(s) 358, 360
NciI CCSGG 2 cut(s) 31, 134
NcoI CCATGG 1 cut(s) 2145
NdeII GATC 6 cut(s) 4, 318, 331, 741, 1250, 1722
NlaIII CATG 8 cut(s) 77, 520, 1114, 1285, 1346, 1703, 2119, 2149
NlaIV GGNNCC 6 cut(s) 6, 29, 88, 457, 1155, 1441
NmeAIII GCCGAG 1 cut(s) 1923
NsbI TGCGCA 1 cut(s) 1875
NsiI ATGCAT 1 cut(s) 2121
NspI RCATGY 2 cut(s) 520, 2119
PaeI GCATGC 2 cut(s) 520, 2119
PagI TCATGA 3 cut(s) 1281, 1342, 1699
PaqCI CACCTGC 1 cut(s) 1774
PasI CCCWGGG 1 cut(s) 1827
PceI AGGCCT 1 cut(s) 524
PciSI GCTCTTC 1 cut(s) 1795
PcsI WCGNNNNNNNCGW 3 cut(s) 198, 270, 327
PdmI GAANNNNTTC 1 cut(s) 1164
PfeI GAWTC 3 cut(s) 98, 337, 2269
PfoI TCCNGGA 1 cut(s) 1771
PkrI GCNGC 9 cut(s) 251, 302, 479, 482, 810, 1093, 1641, 2032, 2319
Ple19I CGATCG 1 cut(s) 334
PleI GAGTC 2 cut(s) 451, 541
PpsI GAGTC 2 cut(s) 451, 541
PsiI TTATAA 1 cut(s) 705
Psp124BI GAGCTC 2 cut(s) 128, 259
Psp6I CCWGG 3 cut(s) 1771, 1826, 1929
PspFI CCCAGC 1 cut(s) 1667
PspGI CCWGG 3 cut(s) 1771, 1826, 1929
PspN4I GGNNCC 6 cut(s) 6, 29, 88, 457, 1155, 1441
PspPI GGNCC 9 cut(s) 27, 342, 456, 985, 1015, 1237, 1439, 1940, 2019
PsrI GAACNNNNNNTAC 2 cut(s) 1365, 1397
PsuI RGATCY 3 cut(s) 4, 1250, 1722
PvuI CGATCG 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 2033
RsaI GTAC 2 cut(s) 659, 890
RsaNI GTAC 2 cut(s) 658, 889
RseI CAYNNNNRTG 2 cut(s) 602, 828
SacI GAGCTC 2 cut(s) 128, 259
SapI GCTCTTC 1 cut(s) 1795
SaqAI TTAA 3 cut(s) 720, 1029, 1049
SatI GCNGC 9 cut(s) 250, 301, 478, 481, 809, 1092, 1640, 2031, 2318
Sau3AI GATC 6 cut(s) 4, 318, 331, 741, 1250, 1722
Sau96I GGNCC 9 cut(s) 27, 342, 456, 985, 1015, 1237, 1439, 1940, 2019
ScaI AGTACT 1 cut(s) 890
SchI GAGTC 2 cut(s) 452, 542
ScrFI CCNGG 5 cut(s) 31, 134, 1773, 1828, 1931
SduI GDGCHC 3 cut(s) 128, 259, 1207
SfaNI GCATC 9 cut(s) 795, 925, 1270, 1296, 1392, 1403, 1898, 2128, 2205
SfcI CTRYAG 2 cut(s) 2139, 2156
SinI GGWCC 5 cut(s) 27, 456, 1015, 1439, 2019
SmiMI CAYNNNNRTG 2 cut(s) 602, 828
SmlI CTYRAG 4 cut(s) 762, 1292, 1310, 1923
SmoI CTYRAG 4 cut(s) 762, 1292, 1310, 1923
SphI GCATGC 2 cut(s) 520, 2119
Sse9I AATT 6 cut(s) 786, 798, 1221, 1539, 1814, 1837
SseBI AGGCCT 1 cut(s) 524
SspI AATATT 2 cut(s) 1042, 1513
SspMI CTAG 2 cut(s) 711, 1728
SstI GAGCTC 2 cut(s) 128, 259
StuI AGGCCT 1 cut(s) 524
StyD4I CCNGG 5 cut(s) 29, 132, 1771, 1826, 1929
StyI CCWWGG 3 cut(s) 237, 856, 2145
TaiI ACGT 3 cut(s) 109, 728, 1130
TaqI TCGA 9 cut(s) 264, 273, 381, 410, 431, 491, 611, 617, 1004
TasI AATT 6 cut(s) 786, 798, 1221, 1539, 1814, 1837
TatI WGTACW 1 cut(s) 888
TauI GCSGC 4 cut(s) 252, 303, 483, 2320
TfiI GAWTC 3 cut(s) 98, 337, 2269
Tru1I TTAA 3 cut(s) 720, 1029, 1049
Tru9I TTAA 3 cut(s) 720, 1029, 1049
TscAI CASTG 6 cut(s) 513, 568, 910, 1963, 2049, 2137
TseI GCWGC 5 cut(s) 477, 808, 1091, 1639, 2030
TspRI CASTG 6 cut(s) 513, 568, 910, 1963, 2049, 2137
VpaK11BI GGWCC 5 cut(s) 27, 456, 1015, 1439, 2019
XagI CCTNNNNNAGG 1 cut(s) 236
XapI RAATTY 2 cut(s) 786, 1837
XceI RCATGY 2 cut(s) 520, 2119
XcmI CCANNNNNNNNNTGG 1 cut(s) 80
XmiI GTMKAC 2 cut(s) 162, 1381
XmnI GAANNNNTTC 1 cut(s) 1164
XspI CTAG 2 cut(s) 711, 1728
ZraI GACGTC 1 cut(s) 726
ZrmI AGTACT 1 cut(s) 890
Zsp2I ATGCAT 1 cut(s) 2121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.