Prupe.1G289000_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0009465
Physical Location & Seq
Forward (+)
28828981 .. 28836826
7846 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G289000.1

Sequence Viewer

Length: 2448 bp
ATGGATCCGATGCCCAACACCCGCCGGACCCGAAAAATCCCACCTAAACCCGAACTCTACTCCACCGTTGTCATCCACGAAGATGGCGATTCCAACTCCGAATCCGATCGAGACCAGAGGCAGCGGCGGAGCAAGCCCAAGAGCTCCGACCCGGAACCCGACCTCTACGCCACAATGGTCTACAAGGGCAATGCTCGAGACGACGAAGACGAAGACGAAGACGACGACGCTTCACTCCCTCCGCTCCTCAAACGCCTTCCCAAAGACTTCGGCGGCGGAGCCTCAATCGATTACTTCGACGACGAGGAGGACGAAAATGGCGGCGATTTCGGCACTATGATCATCAAGCCCGATCGCAATCGGACCACGGGGAGGTCACGTGACTTCAAGCGAGGCTCCATCGACGATGACGGAGATGGCGATGGGTTCTCCACTTTTGTGGTCAGGTCGTCGAGCGAGAGAGAGTCGATAAGTGGGACCGTGGTGAGGAGGACGAGCAGCGGCGCTGGTTCGACGATGAGTAGGGCGGTGGCGAGCATGCAGGCCTCGTCGGAGTTAGGGTTTGGGAAGCAGAGGAGGGGCAGTGGATCCTCGCAAGGCGAAGAGTATCGCCAAACCACTAAAATGTCGTCGAGTTCCATACCCGATAGCGTTACCAGAGAAGACCCCACTGTAAAGTACGAGTTGCTCAATGAACTTGGGAAGGGGTCTTATGGCGCGGTTTATAAAGCTAGGGATATAAAGACTTCGGAGCTCGTGGCAATCAAAGTCATTTCCTTGTCTCAAGGGGAGGAGGGGTATGAAGAAATTTGTGGTGAAATTGAGATGCTGCAGCAATGCAATCACCCTAATGTTGTTCGGTACCTGGGGAGCTACCAAGGAGAAGAGTATCTTTGGATTGTGATGGAGTACTGTGGTGGTGGAAGTGTTGCAGACTTGATGAATGTTACTGAGGATGCTTTGGAGGAGTACCAAATAGCCTACATTTGTAGGGAGGCATTGAAGGGCCTTGCTTATTTGCACTCCATTTTCAAGGTCCACAGAGATATAAAGGGTGGTAATATTCTGTTAACTGAACAAGGAGATGTGAAGTTGGGTGATTTTGGGGTTGCAGCACAGCTAACAAGAACCATGTCCAAGCGCAACACGTTCATTGGCACTCCACATTGGATGGCTCCAGAAGTTATTCAGGAAAGCCGCTATGATGGAAAGGTGGATGTATGGGCTCTTGGTGTTTCTGCAATTGAAATGGCAGAGGGGCTCCCTCCAAGATCTTCTGTGCATCCAATGAGGGTTTTATTCATGATATCCCTTGAGCCAGCCCCGATGCTTGAGGATAAAGAAAAATGGTCTCTCGTGTTTCATGATTTTGTGGCGAAGTGCCTCACAAAAGAACCTCGTCTACGTCCTACTGCATCTGAGATGCTGAAGCACAAATTCATCGAAAAATGCAAGTGTGGTCCCTCTGCAATGCTGGCAAAGATTGAAAAAGCAAGGCAAATTAGGGCTTCCATGGCTTTGCAAGCACAAAGTATTGCTCCAGTTGAACCGGAAGATAGTACACTTGTAGTTTCAAAAGTCAATGAGGATTATGGAGATACTGTTCCTTCAAGGCCTAATAATCAAGTGGAAAATGAAGTATCTACAGCTAGCACGTTGAGGAAGCAGCACATATCTGGTGATGCAGGACTGGCCGGGGAAGGTAATTTTGGCACTGTAATAATTCACGGTGGAGATGAAAGAGATGAGACTGCTAATCAGACACAAAGTTTTAATGTCAAAGAACCTGCAGCTGGTCCAGGATTTCTTGAAAATCCTTCCAATACTGGCACAGGAGGTAAACCAGCTGAACCCAGGGTGGAAAATGCTGGAGGTGTTTCCTTACATAGCATTTCGGTTGGAGAACCACATTCAGTTACACAGACCATACAGGCATCTTCTCGTTCAATTCTTGGCTCTTCTGAGCAGAACCTCAAGACAAAAGGACAGGTTGAAGGTCAAAGCAGTGGCACATTGAAGAATGAAACTGTCAACCGGAAAGCTTTTGCAATGCAAGATAAGCTTTGGTCCATATATGCAGCTGGTAATACAGTGCCTATTCCATTTTTGAGGGCAACGGATATATCCCCCATTGCTCTCTTATCAGATAATGTTCTTGGAGGCATGCATGAGGATAATAATGGAAGTGTAGCCGTAGAGGCTCTACAGGAGCTTTTTACTGGTGATGGACAGTCTAAAAAGGGACGAAGAGGACAAAATGAGATGCCCCTCCCTCCAAGTGTTTACAAAAGGCTCTTTACAAGTTCAACTCTAATGAATCTCGCACAGGCTTTAGCCTACCACAAGATGTGCTACGAAGATATGCCGCTCCAAGAGTTGCAAGCAACTCAGGAACAACAGACGATTCAAAATCTTTGTGATACACTAAGAACCATTCTTAGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006950 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007266 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0009893 GO:0009966 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0012501 GO:0016043 GO:0016301 GO:0016310 GO:0016477 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017048 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023051 GO:0023052 GO:0030029 GO:0030036 GO:0031098 GO:0031267 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0040011 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043408 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048365 GO:0048518 GO:0048522 GO:0048583 GO:0048870 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051674 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902531
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

816

Amino Acids

89.46

Weight (kDa)

5.19

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
Acc36I ACCTGC 1 cut(s) 1795
Acc65I GGTACC 1 cut(s) 861
AccB1I GGYRCC 1 cut(s) 861
AccBSI CCGCTC 2 cut(s) 244, 2368
AccI GTMKAC 2 cut(s) 180, 1402
AccII CGCG 1 cut(s) 719
AclWI GGATC 3 cut(s) 12, 582, 595
AcoI YGGCCR 1 cut(s) 1692
AcsI RAATTY 2 cut(s) 807, 1436
AcuI CTGAAG 1 cut(s) 1448
AcvI CACGTG 1 cut(s) 380
AfaI GTAC 5 cut(s) 680, 863, 911, 971, 1561
AfiI CCNNNNNNNGG 3 cut(s) 372, 486, 1793
AflIII ACRYGT 1 cut(s) 1146
AjnI CCWGG 3 cut(s) 864, 1798, 1853
AjuI GAANNNNNNNTTGG 2 cut(s) 1692, 1724
AleI CACNNNNGTG 1 cut(s) 437
AloI GAACNNNNNNTCC 2 cut(s) 1587, 1619
Alw21I GWGCWC 2 cut(s) 146, 756
Alw26I GTCTC 5 cut(s) 105, 192, 786, 1356, 1742
AlwI GGATC 3 cut(s) 12, 582, 595
Ama87I CYCGRG 1 cut(s) 195
AoxI GGCC 4 cut(s) 543, 1006, 1613, 1692
ApeKI GCWGC 8 cut(s) 121, 498, 829, 832, 1112, 1666, 1790, 2078
ApoI RAATTY 2 cut(s) 807, 1436
Asp700I GAANNNNTTC 1 cut(s) 1185
Asp718I GGTACC 1 cut(s) 861
AspLEI GCGC 3 cut(s) 506, 719, 1143
AspS9I GGNCC 8 cut(s) 27, 363, 477, 1006, 1036, 1460, 1796, 2067
AsuC2I CCSGG 2 cut(s) 152, 1696
AsuHPI GGTGA 6 cut(s) 496, 827, 836, 1109, 1691, 2234
AsuNHI GCTAGC 1 cut(s) 1649
AvaI CYCGRG 1 cut(s) 195
AvaII GGWCC 7 cut(s) 27, 363, 477, 1036, 1460, 1796, 2067
BamHI GGATCC 2 cut(s) 4, 587
BanI GGYRCC 1 cut(s) 861
BanII GRGCYC 4 cut(s) 146, 756, 1228, 1263
BauI CACGAG 2 cut(s) 755, 1355
BbrPI CACGTG 1 cut(s) 380
BbsI GAAGAC 4 cut(s) 213, 219, 225, 669
Bbv12I GWGCWC 2 cut(s) 146, 756
BbvI GCAGC 8 cut(s) 133, 510, 816, 844, 1124, 1678, 1802, 2090
BccI CCATC 8 cut(s) 77, 407, 410, 416, 898, 1165, 1199, 2219
BceAI ACGGC 1 cut(s) 2177
BciT130I CCWGG 3 cut(s) 866, 1800, 1855
BclI TGATCA 1 cut(s) 339
BcnI CCSGG 2 cut(s) 152, 1696
BcoDI GTCTC 5 cut(s) 105, 192, 786, 1356, 1742
BfaI CTAG 2 cut(s) 732, 1650
BfmI CTRYAG 4 cut(s) 830, 1644, 1788, 2204
BfoI RGCGCY 1 cut(s) 507
BfuAI ACCTGC 1 cut(s) 1795
BglI GCCNNNNNGGC 1 cut(s) 2198
BglII AGATCT 1 cut(s) 1271
BmcAI AGTACT 1 cut(s) 911
Bme1390I CCNGG 5 cut(s) 152, 866, 1696, 1800, 1855
Bme18I GGWCC 7 cut(s) 27, 363, 477, 1036, 1460, 1796, 2067
BmeT110I CYCGRG 1 cut(s) 195
BmgT120I GGNCC 8 cut(s) 27, 363, 477, 1006, 1036, 1460, 1796, 2067
BmrFI CCNGG 5 cut(s) 152, 866, 1696, 1800, 1855
BmsI GCATC 9 cut(s) 816, 946, 1291, 1317, 1413, 1424, 1672, 1943, 2253
BmtI GCTAGC 1 cut(s) 1653
BpiI GAAGAC 4 cut(s) 213, 219, 225, 669
BpmI CTGGAG 3 cut(s) 1161, 1524, 1890
BpuEI CTTGAG 4 cut(s) 768, 1334, 1352, 1958
BpuMI CCSGG 2 cut(s) 152, 1696
Bsa29I ATCGAT 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 380
BsaBI GATNNNNATC 1 cut(s) 357
BsaI GGTCTC 2 cut(s) 105, 1356
BsaJI CCNNGG 8 cut(s) 366, 480, 865, 877, 1512, 1695, 1853, 1854
BsaWI WCCGGW 2 cut(s) 1549, 2034
Bsc4I CCNNNNNNNGG 3 cut(s) 372, 486, 1793
Bse1I ACTGG 4 cut(s) 1541, 1695, 1831, 2224
Bse3DI GCAATG 5 cut(s) 196, 842, 1476, 2055, 2130
Bse8I GATNNNNATC 1 cut(s) 357
BseBI CCWGG 3 cut(s) 866, 1800, 1855
BseCI ATCGAT 1 cut(s) 288
BseDI CCNNGG 8 cut(s) 366, 480, 865, 877, 1512, 1695, 1853, 1854
BseGI GGATG 5 cut(s) 72, 961, 1176, 1222, 1282
BseJI GATNNNNATC 1 cut(s) 357
BseLI CCNNNNNNNGG 3 cut(s) 372, 486, 1793
BseMI GCAATG 5 cut(s) 196, 842, 1476, 2055, 2130
BseMII CTCAG 4 cut(s) 942, 1410, 1953, 2402
BseNI ACTGG 4 cut(s) 1541, 1695, 1831, 2224
BseRI GAGGAG 6 cut(s) 236, 320, 502, 589, 806, 980
BseXI GCAGC 8 cut(s) 133, 510, 816, 844, 1124, 1678, 1802, 2090
Bsh1236I CGCG 1 cut(s) 719
Bsh1285I CGRYCG 2 cut(s) 109, 355
BshFI GGCC 4 cut(s) 545, 1008, 1615, 1694
BshNI GGYRCC 1 cut(s) 861
BshVI ATCGAT 1 cut(s) 288
BsiEI CGRYCG 2 cut(s) 109, 355
BsiHKAI GWGCWC 2 cut(s) 146, 756
BsiHKCI CYCGRG 1 cut(s) 195
BsiSI CCGG 5 cut(s) 25, 152, 1550, 1695, 2035
BslFI GGGAC 3 cut(s) 490, 1446, 2256
BslI CCNNNNNNNGG 3 cut(s) 372, 486, 1793
BsmAI GTCTC 5 cut(s) 105, 192, 786, 1356, 1742
BsmBI CGTCTC 1 cut(s) 192
BsmFI GGGAC 3 cut(s) 490, 1446, 2256
BsnI GGCC 4 cut(s) 545, 1008, 1615, 1694
Bso31I GGTCTC 2 cut(s) 105, 1356
BsoBI CYCGRG 1 cut(s) 195
Bsp1286I GDGCHC 4 cut(s) 146, 756, 1228, 1263
Bsp143I GATC 6 cut(s) 4, 106, 339, 352, 587, 1271
Bsp19I CCATGG 1 cut(s) 1512
BspANI GGCC 4 cut(s) 545, 1008, 1615, 1694
BspCNI CTCAG 4 cut(s) 943, 1411, 1954, 2401
BspDI ATCGAT 1 cut(s) 288
BspFNI CGCG 1 cut(s) 719
BspHI TCATGA 2 cut(s) 1302, 1363
BspMAI CTGCAG 2 cut(s) 834, 1792
BspMI ACCTGC 1 cut(s) 1795
BspOI GCTAGC 1 cut(s) 1653
BspPI GGATC 3 cut(s) 12, 582, 595
BspQI GCTCTTC 1 cut(s) 1963
BspT107I GGYRCC 1 cut(s) 861
BspTNI GGTCTC 2 cut(s) 105, 1356
BsrBI CCGCTC 2 cut(s) 244, 2368
BsrDI GCAATG 5 cut(s) 196, 842, 1476, 2055, 2130
BsrI ACTGG 4 cut(s) 1541, 1695, 1831, 2224
BssECI CCNNGG 8 cut(s) 366, 480, 865, 877, 1512, 1695, 1853, 1854
BssMI GATC 6 cut(s) 4, 106, 339, 352, 587, 1271
BssSI CACGAG 2 cut(s) 755, 1355
BssT1I CCWWGG 2 cut(s) 877, 1512
Bst2BI CACGAG 2 cut(s) 755, 1355
Bst2UI CCWGG 3 cut(s) 866, 1800, 1855
Bst6I CTCTTC 4 cut(s) 597, 879, 1963, 2242
BstBAI YACGTR 1 cut(s) 380
BstDEI CTNAG 6 cut(s) 951, 1419, 1962, 2388, 2426, 2438
BstDSI CCRYGG 3 cut(s) 366, 480, 1512
BstF5I GGATG 5 cut(s) 72, 961, 1176, 1222, 1282
BstFNI CGCG 1 cut(s) 719
BstH2I RGCGCY 1 cut(s) 507
BstHHI GCGC 3 cut(s) 506, 719, 1143
BstKTI GATC 6 cut(s) 7, 109, 342, 355, 590, 1274
BstMAI GTCTC 5 cut(s) 105, 192, 786, 1356, 1742
BstMBI GATC 6 cut(s) 4, 106, 339, 352, 587, 1271
BstMCI CGRYCG 2 cut(s) 109, 355
BstMWI GCNNNNNNNGC 8 cut(s) 133, 330, 1475, 1514, 1523, 1691, 2059, 2198
BstNI CCWGG 3 cut(s) 866, 1800, 1855
BstNSI RCATGY 2 cut(s) 541, 2167
BstSCI CCNGG 5 cut(s) 150, 864, 1694, 1798, 1853
BstSFI CTRYAG 4 cut(s) 830, 1644, 1788, 2204
BstUI CGCG 1 cut(s) 719
BstV1I GCAGC 8 cut(s) 133, 510, 816, 844, 1124, 1678, 1802, 2090
BstV2I GAAGAC 4 cut(s) 213, 219, 225, 669
BstX2I RGATCY 3 cut(s) 4, 587, 1271
BstXI CCANNNNNNTGG 2 cut(s) 83, 439
BstYI RGATCY 3 cut(s) 4, 587, 1271
Bsu15I ATCGAT 1 cut(s) 288
BsuRI GGCC 4 cut(s) 545, 1008, 1615, 1694
BsuTUI ATCGAT 1 cut(s) 288
BtgI CCRYGG 3 cut(s) 366, 480, 1512
BtgZI GCGATG 1 cut(s) 435
BtsCI GGATG 5 cut(s) 72, 961, 1176, 1222, 1282
BtsI GCAGTG 2 cut(s) 589, 2011
BtsIMutI CAGTG 5 cut(s) 589, 669, 1713, 2011, 2097
BveI ACCTGC 1 cut(s) 1795
CciI TCATGA 2 cut(s) 1302, 1363
CfoI GCGC 3 cut(s) 506, 719, 1143
Cfr13I GGNCC 8 cut(s) 27, 363, 477, 1006, 1036, 1460, 1796, 2067
ClaI ATCGAT 1 cut(s) 288
CseI GACGC 1 cut(s) 236
Csp6I GTAC 5 cut(s) 679, 862, 910, 970, 1560
CviAII CATG 7 cut(s) 538, 1132, 1303, 1364, 1513, 2164, 2168
CviQI GTAC 5 cut(s) 679, 862, 910, 970, 1560
DdeI CTNAG 6 cut(s) 951, 1419, 1962, 2388, 2426, 2438
DpnI GATC 6 cut(s) 6, 108, 341, 354, 589, 1273
DpnII GATC 6 cut(s) 4, 106, 339, 352, 587, 1271
EaeI YGGCCR 1 cut(s) 1692
Eam1104I CTCTTC 4 cut(s) 597, 879, 1963, 2242
EarI CTCTTC 4 cut(s) 597, 879, 1963, 2242
EciI GGCGGA 2 cut(s) 142, 291
Ecl136II GAGCTC 2 cut(s) 144, 754
Eco130I CCWWGG 2 cut(s) 877, 1512
Eco147I AGGCCT 2 cut(s) 545, 1615
Eco24I GRGCYC 4 cut(s) 146, 756, 1228, 1263
Eco31I GGTCTC 2 cut(s) 105, 1356
Eco32I GATATC 1 cut(s) 1308
Eco47I GGWCC 7 cut(s) 27, 363, 477, 1036, 1460, 1796, 2067
Eco53kI GAGCTC 2 cut(s) 144, 754
Eco57I CTGAAG 1 cut(s) 1448
Eco72I CACGTG 1 cut(s) 380
Eco88I CYCGRG 1 cut(s) 195
EcoICRI GAGCTC 2 cut(s) 144, 754
EcoO109I RGGNCCY 1 cut(s) 1006
EcoRII CCWGG 3 cut(s) 864, 1798, 1853
EcoRV GATATC 1 cut(s) 1308
EcoT14I CCWWGG 2 cut(s) 877, 1512
EcoT22I ATGCAT 1 cut(s) 2169
EcoT38I GRGCYC 4 cut(s) 146, 756, 1228, 1263
ErhI CCWWGG 2 cut(s) 877, 1512
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 7 cut(s) 541, 1135, 1306, 1367, 1516, 2167, 2171
FalI AAGNNNNNCTT 4 cut(s) 876, 908, 2046, 2078
FaqI GGGAC 3 cut(s) 490, 1446, 2256
FatI CATG 7 cut(s) 537, 1131, 1302, 1363, 1512, 2163, 2167
FauI CCCGC 1 cut(s) 29
FbaI TGATCA 1 cut(s) 339
FblI GTMKAC 2 cut(s) 180, 1402
FokI GGATG 5 cut(s) 59, 968, 1183, 1229, 1269
FriOI GRGCYC 4 cut(s) 146, 756, 1228, 1263
FspBI CTAG 2 cut(s) 732, 1650
GlaI GCGC 3 cut(s) 505, 718, 1142
GsuI CTGGAG 3 cut(s) 1161, 1524, 1890
HaeII RGCGCY 1 cut(s) 507
HaeIII GGCC 4 cut(s) 545, 1008, 1615, 1694
HapII CCGG 5 cut(s) 25, 152, 1550, 1695, 2035
HgaI GACGC 1 cut(s) 236
HhaI GCGC 3 cut(s) 506, 719, 1143
Hin1II CATG 7 cut(s) 541, 1135, 1306, 1367, 1516, 2167, 2171
Hin6I GCGC 3 cut(s) 504, 717, 1141
HinP1I GCGC 3 cut(s) 504, 717, 1141
HincII GTYRAC 2 cut(s) 1071, 2032
HindII GTYRAC 2 cut(s) 1071, 2032
HindIII AAGCTT 2 cut(s) 2040, 2060
HinfI GANTC 5 cut(s) 89, 101, 464, 2317, 2404
HpaI GTTAAC 1 cut(s) 1071
HpaII CCGG 5 cut(s) 25, 152, 1550, 1695, 2035
HphI GGTGA 6 cut(s) 496, 827, 836, 1109, 1691, 2234
Hpy166II GTNNAC 8 cut(s) 181, 1039, 1071, 1403, 1562, 1841, 2032, 2284
Hpy188III TCNNGA 9 cut(s) 110, 197, 1178, 1190, 1303, 1364, 1808, 1975, 2390
Hpy8I GTNNAC 8 cut(s) 181, 1039, 1071, 1403, 1562, 1841, 2032, 2284
HpyAV CCTTC 7 cut(s) 266, 697, 997, 1617, 1694, 1827, 1988
HpyCH4IV ACGT 4 cut(s) 379, 1148, 1405, 1655
HpyF10VI GCNNNNNNNGC 8 cut(s) 133, 330, 1475, 1514, 1523, 1691, 2059, 2198
HpyF3I CTNAG 6 cut(s) 951, 1419, 1962, 2388, 2426, 2438
HpySE526I ACGT 4 cut(s) 379, 1148, 1405, 1655
Hsp92II CATG 7 cut(s) 541, 1135, 1306, 1367, 1516, 2167, 2171
HspAI GCGC 3 cut(s) 504, 717, 1141
KpnI GGTACC 1 cut(s) 865
Ksp22I TGATCA 1 cut(s) 339
KspAI GTTAAC 1 cut(s) 1071
Kzo9I GATC 6 cut(s) 4, 106, 339, 352, 587, 1271
LguI GCTCTTC 1 cut(s) 1963
Lsp1109I GCAGC 8 cut(s) 133, 510, 816, 844, 1124, 1678, 1802, 2090
LweI GCATC 9 cut(s) 816, 946, 1291, 1317, 1413, 1424, 1672, 1943, 2253
MaeI CTAG 2 cut(s) 732, 1650
MaeII ACGT 4 cut(s) 379, 1148, 1405, 1655
MaeIII GTNAC 5 cut(s) 375, 380, 652, 946, 1915
MalI GATC 6 cut(s) 6, 108, 341, 354, 589, 1273
MbiI CCGCTC 2 cut(s) 244, 2368
MboI GATC 6 cut(s) 4, 106, 339, 352, 587, 1271
MfeI CAATTG 1 cut(s) 1242
MflI RGATCY 3 cut(s) 4, 587, 1271
MhlI GDGCHC 4 cut(s) 146, 756, 1228, 1263
MluCI AATT 8 cut(s) 807, 819, 1242, 1436, 1500, 1705, 1722, 1947
MlyI GAGTC 1 cut(s) 473
MmeI TCCRAC 4 cut(s) 117, 171, 531, 1879
Mph1103I ATGCAT 1 cut(s) 2169
MroXI GAANNNNTTC 1 cut(s) 1185
MseI TTAA 2 cut(s) 1070, 1773
MslI CAYNNNNRTG 4 cut(s) 81, 437, 623, 849
MspA1I CMGCKG 5 cut(s) 124, 501, 1793, 1847, 2081
MspI CCGG 5 cut(s) 25, 152, 1550, 1695, 2035
MspR9I CCNGG 5 cut(s) 152, 866, 1696, 1800, 1855
MunI CAATTG 1 cut(s) 1242
MvaI CCWGG 3 cut(s) 866, 1800, 1855
MvnI CGCG 1 cut(s) 719
MwoI GCNNNNNNNGC 8 cut(s) 133, 330, 1475, 1514, 1523, 1691, 2059, 2198
NciI CCSGG 2 cut(s) 152, 1696
NcoI CCATGG 1 cut(s) 1512
NdeII GATC 6 cut(s) 4, 106, 339, 352, 587, 1271
NheI GCTAGC 1 cut(s) 1649
NlaIII CATG 7 cut(s) 541, 1135, 1306, 1367, 1516, 2167, 2171
NmuCI GTSAC 2 cut(s) 375, 380
NsiI ATGCAT 1 cut(s) 2169
NspI RCATGY 2 cut(s) 541, 2167
OliI CACNNNNGTG 1 cut(s) 437
PaeI GCATGC 2 cut(s) 541, 2167
PaeR7I CTCGAG 1 cut(s) 195
PagI TCATGA 2 cut(s) 1302, 1363
PasI CCCWGGG 1 cut(s) 1854
PceI AGGCCT 2 cut(s) 545, 1615
PciSI GCTCTTC 1 cut(s) 1963
PcsI WCGNNNNNNNCGW 6 cut(s) 84, 207, 222, 294, 309, 417
PdmI GAANNNNTTC 1 cut(s) 1185
PfeI GAWTC 4 cut(s) 89, 101, 2317, 2404
PfoI TCCNGGA 1 cut(s) 1798
Ple19I CGATCG 2 cut(s) 109, 355
PleI GAGTC 1 cut(s) 472
PmaCI CACGTG 1 cut(s) 380
PmlI CACGTG 1 cut(s) 380
PpsI GAGTC 1 cut(s) 472
Ppu21I YACGTR 1 cut(s) 380
PsiI TTATAA 1 cut(s) 726
Psp124BI GAGCTC 2 cut(s) 146, 756
Psp6I CCWGG 3 cut(s) 864, 1798, 1853
PspCI CACGTG 1 cut(s) 380
PspGI CCWGG 3 cut(s) 864, 1798, 1853
PspPI GGNCC 8 cut(s) 27, 363, 477, 1006, 1036, 1460, 1796, 2067
PsrI GAACNNNNNNTAC 2 cut(s) 1386, 1418
PstI CTGCAG 2 cut(s) 834, 1792
PsuI RGATCY 3 cut(s) 4, 587, 1271
PvuI CGATCG 2 cut(s) 109, 355
PvuII CAGCTG 3 cut(s) 1793, 1847, 2081
RsaI GTAC 5 cut(s) 680, 863, 911, 971, 1561
RsaNI GTAC 5 cut(s) 679, 862, 910, 970, 1560
RseI CAYNNNNRTG 4 cut(s) 81, 437, 623, 849
SacI GAGCTC 2 cut(s) 146, 756
SapI GCTCTTC 1 cut(s) 1963
SaqAI TTAA 2 cut(s) 1070, 1773
Sau3AI GATC 6 cut(s) 4, 106, 339, 352, 587, 1271
Sau96I GGNCC 8 cut(s) 27, 363, 477, 1006, 1036, 1460, 1796, 2067
ScaI AGTACT 1 cut(s) 911
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 5 cut(s) 152, 866, 1696, 1800, 1855
SduI GDGCHC 4 cut(s) 146, 756, 1228, 1263
SfaNI GCATC 9 cut(s) 816, 946, 1291, 1317, 1413, 1424, 1672, 1943, 2253
SfcI CTRYAG 4 cut(s) 830, 1644, 1788, 2204
Sfr274I CTCGAG 1 cut(s) 195
SinI GGWCC 7 cut(s) 27, 363, 477, 1036, 1460, 1796, 2067
SlaI CTCGAG 1 cut(s) 195
SmiMI CAYNNNNRTG 4 cut(s) 81, 437, 623, 849
SmlI CTYRAG 5 cut(s) 195, 783, 1313, 1331, 1973
SmoI CTYRAG 5 cut(s) 195, 783, 1313, 1331, 1973
SphI GCATGC 2 cut(s) 541, 2167
Sse9I AATT 8 cut(s) 807, 819, 1242, 1436, 1500, 1705, 1722, 1947
SseBI AGGCCT 2 cut(s) 545, 1615
SspI AATATT 1 cut(s) 1063
SspMI CTAG 2 cut(s) 732, 1650
SstI GAGCTC 2 cut(s) 146, 756
StuI AGGCCT 2 cut(s) 545, 1615
StyD4I CCNGG 5 cut(s) 150, 864, 1694, 1798, 1853
StyI CCWWGG 2 cut(s) 877, 1512
TaiI ACGT 4 cut(s) 382, 1151, 1408, 1658
TasI AATT 8 cut(s) 807, 819, 1242, 1436, 1500, 1705, 1722, 1947
TatI WGTACW 2 cut(s) 909, 1559
TauI GCSGC 6 cut(s) 127, 276, 324, 504, 1200, 2368
TfiI GAWTC 4 cut(s) 89, 101, 2317, 2404
Tru1I TTAA 2 cut(s) 1070, 1773
Tru9I TTAA 2 cut(s) 1070, 1773
TscAI CASTG 5 cut(s) 589, 676, 1720, 2011, 2097
TseFI GTSAC 2 cut(s) 375, 380
TseI GCWGC 8 cut(s) 121, 498, 829, 832, 1112, 1666, 1790, 2078
Tsp45I GTSAC 2 cut(s) 375, 380
TspGWI ACGGA 2 cut(s) 426, 2132
TspRI CASTG 5 cut(s) 589, 676, 1720, 2011, 2097
VpaK11BI GGWCC 7 cut(s) 27, 363, 477, 1036, 1460, 1796, 2067
XapI RAATTY 2 cut(s) 807, 1436
XceI RCATGY 2 cut(s) 541, 2167
XhoI CTCGAG 1 cut(s) 195
XmiI GTMKAC 2 cut(s) 180, 1402
XmnI GAANNNNTTC 1 cut(s) 1185
XspI CTAG 2 cut(s) 732, 1650
ZrmI AGTACT 1 cut(s) 911
Zsp2I ATGCAT 1 cut(s) 2169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.