MD16G1059600.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009465
Physical Location & Seq
Reverse (-)
4287259 .. 4294354
7096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1059600.v1.1.491

Sequence Viewer

Length: 2391 bp
ATGGATCCGATCCTCACCACTCGCCGGACCCGAAAAATCCCACCCAAACCCGATTACTCAACGGTAGTCATCCACGACGATGATGACGCCGAGTCCAATCGACAGAGCAAGCACAAGAGCTCCGACCCGGAACCCGACCTCTACGCCACCATGGTCTATAAGGGTAATGCCGACGAAGACGAAGACGACGACGACGCCTCTCTCCCTCCGCTCCTCAAACGCCTCCCCAAGGACTTCGGCGGCGGACCCTCAATCGATTACTTCGACGACGAAGAGGAAGACGAAAACGGCGGCGATTTCGGCACAATGATCATTAAGCCCGATCGAAACCGGACCACGAGGAGGTCGCGCGACTTCAAGCGGGCGTCGTTCGACGATGACGGAGAAGGCAGTGGGTTCTCGACCTTTGTGGTGAGGCCGTCGAGCGAGAGAGAGTCGGTCAGCGGGACCGTGGTGAGGAGGACGAGCAGCGGCGCTGGTTCGACGATGAGTAGGGCAGTGGCGAGTATGCAGGCCTCGTCGGAGTTAGGAGTTGGGAAGCAGAGGAGGGGCAGTGGGTCGTCGCAGGGAGAAGAGTATCGGCAAACCACTAAAATGTCGTCGAGTTCGATACCAGATAGCATTACCAGAGAAGACCCAACTGTAAAGTACGAGCTGCTCAATGAGCTTGGGAAGGGATCTTATGGCGAGGTTTATAAAGCCAGGGATATTAAGACGTCGGAGCTGGTGGCGATCAAAGTCATTTCATTGTCTCAAGAGGAAGAGGGGTATGAAGAAATTTGCGGAGAAATCGAGATGCTGCAACAATGTAACCATCCGAATGTGGTTCGGTACCTGGGGAGCTACCAAGGAGAAGAGTATCTTTGGATTGTGATGGAGTACTGTGGTGGTGGTAGTGTTGCCGACTTGATCAATGCTACCGAGGATGCTCTGGAGGAGTATCAAATAGCCTACATTTGTAGGGAAGCTTTGAAGGGCCTTGCGTATCTGCACTCGATTTTCAAGGTCCACAGAGATATTAAGGGTGGTAATATTCTGTTAACTGAACAAGGAGATGTCAAGTTGGGTGATTTTGGTGTTGCAGCTCAACTTACAAGAACCATGTCAAAGCGCAACACGTTCATCGGCACTCCACATTGGATGGCTCCAGAAGTTATTCAGGAAAGTCGCTACGACGGAAAGGTGGATGTATGGGCTCTTGGTGTTTCTGCAATTGAAATGGCAGAGGGGCTCCCTCCAAGATCTTCAGTGCATCCAATGAGGGTTATATTCATGATATCCCTTGAGCCAGCCCCGATGCTTGAGGATAAAGAAAAATGGTCTCTTGTGTTTCATGATTTTGTGGCAAAATGCCTAACTAAAGAACCTCGTCTACGCCCTACTGCATCTGAGATGCTTAAGCACAAGTTCATTGAAAAATGCCAATGTGGTCCCTCCGTAATGTTGGCAAAGATTGAAAAGGCAAGGCAAGCAAGGGCTTCAATGGCTTTGGAAGCACAACATATTGCTCCACCTGAACGGGATAGTACAATTGAAGCTCCCAAAGTCAATGAGGATTATGGAGATACTGTTCCTTCAAAGCCACTCCAAGTGGAAAATGAAGTATCTTCAGGTAGCACATTGAGGAAGCAGCACATATCTGGTGATGTGGGAATGGCTGGGGAAGGTAACTTTGGGACGTTTATTATTCATGAGGGGGATGAAAAAGATGAGATGTCTAGCGAGACAGAGAGTTTTAGTGTCAAAGAACCTTCACCTGCTCCTGGACTTCCTGAAAGCTCTTCCAATACTGGCGCAGGAGGCAAATTACCCAAACCCAGGGAGGAAAATTCAAGTAGTATTACCAAAAACGGCATTCCAGTTACGCACACTCTACAGGCATCTGAGCAGAGCATAAAGACAAAAAGCACATCTCAAGTCCAGGTTGGAGGTCCAAGCAGTATAAGCAGTGGCAAAGTGAAGAATGAAGCTGTCAGCCGGAAAGCTTTTGCAATGCAAGATAAGCTTTGGTCCATATATGCAGCTGGTAATACAGTGCCTATTCCATTTTTGAGGGCAACCGATATATCCCCCATTGCTCTCTTATCAGACAATGTTCTTGGAGGCATGCATCAGGATAATAGTGGATCTGTAGCCGTGGAGGCACTACAGGAGCTTTTTACTGGTGATGGACAGTCTAAGAAGGGACGAAGAGGGCAAAACGAGATGCCCCTCCCTCCATGTGTTTACAAAAGGCTCTTTACAAGTTCAACTCTAATGAATCTGGCACAAGCGTTAGCCTACCACAAGATGTGTTACGAAGATATGCCGCTTCAAGAAATGCAAGCAACTCAGGAGCAACAGACCATTCAAAATCTTTGTGATACGCTAAGAACCATTCTTAGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006950 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007266 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0009893 GO:0009966 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0012501 GO:0016043 GO:0016301 GO:0016310 GO:0016477 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017048 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023051 GO:0023052 GO:0030029 GO:0030036 GO:0031098 GO:0031267 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0040011 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043408 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048365 GO:0048518 GO:0048522 GO:0048583 GO:0048870 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051674 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902531
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

797

Amino Acids

87.61

Weight (kDa)

5.19

Isoelectric Point (pI)

55.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 217 - 471 2e-70 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 219 - 467 4.9e-51 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 289 - 368 2.3e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AarI CACCTGC 1 cut(s) 1765
AatII GACGTC 1 cut(s) 719
Acc36I ACCTGC 1 cut(s) 1765
Acc65I GGTACC 1 cut(s) 831
AccB1I GGYRCC 1 cut(s) 831
AccBSI CCGCTC 1 cut(s) 211
AccI GTMKAC 1 cut(s) 1372
AccII CGCG 2 cut(s) 349, 351
AciI CCGC 9 cut(s) 209, 240, 243, 291, 361, 444, 471, 783, 2309
AclWI GGATC 4 cut(s) 4, 12, 685, 2134
AcsI RAATTY 2 cut(s) 777, 1828
AcuI CTGAAG 2 cut(s) 1230, 1593
AcyI GRCGYC 4 cut(s) 87, 195, 365, 716
AfaI GTAC 4 cut(s) 650, 833, 881, 1528
AfiI CCNNNNNNNGG 6 cut(s) 24, 229, 342, 456, 1763, 1818
AflII CTTAAG 1 cut(s) 1397
AflIII ACRYGT 1 cut(s) 1116
AhdI GACNNNNNGTC 1 cut(s) 91
AjnI CCWGG 5 cut(s) 701, 834, 1762, 1817, 1920
AjuI GAANNNNNNNTTGG 2 cut(s) 1656, 1688
AloI GAACNNNNNNTCC 2 cut(s) 1554, 1586
Alw21I GWGCWC 1 cut(s) 122
Alw26I GTCTC 3 cut(s) 756, 1326, 1718
AlwI GGATC 4 cut(s) 4, 12, 685, 2134
AoxI GGCC 3 cut(s) 416, 513, 976
ApeKI GCWGC 6 cut(s) 468, 655, 799, 1082, 1630, 2021
ApoI RAATTY 2 cut(s) 777, 1828
Asp700I GAANNNNTTC 1 cut(s) 1155
Asp718I GGTACC 1 cut(s) 831
AspLEI GCGC 4 cut(s) 351, 476, 1113, 1796
AspS9I GGNCC 9 cut(s) 27, 245, 333, 447, 976, 1006, 1430, 1931, 2010
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 7 cut(s) 7, 424, 466, 1079, 1655, 1746, 2177
AvaII GGWCC 8 cut(s) 27, 245, 333, 447, 1006, 1430, 1931, 2010
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 831
BanII GRGCYC 3 cut(s) 122, 1198, 1233
BauI CACGAG 1 cut(s) 337
BbsI GAAGAC 4 cut(s) 183, 189, 285, 639
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 6 cut(s) 480, 642, 786, 1094, 1642, 2033
BccI CCATC 4 cut(s) 822, 868, 1135, 2162
BceAI ACGGC 4 cut(s) 304, 403, 1867, 2120
BciT130I CCWGG 5 cut(s) 703, 836, 1764, 1819, 1922
BclI TGATCA 2 cut(s) 309, 909
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 3 cut(s) 756, 1326, 1718
BfaI CTAG 1 cut(s) 1719
BfmI CTRYAG 3 cut(s) 1874, 2130, 2147
BfoI RGCGCY 1 cut(s) 477
BfrI CTTAAG 1 cut(s) 1397
BfuAI ACCTGC 1 cut(s) 1765
BglI GCCNNNNNGGC 1 cut(s) 2141
BglII AGATCT 1 cut(s) 1241
BmcAI AGTACT 1 cut(s) 881
Bme1390I CCNGG 6 cut(s) 128, 703, 836, 1764, 1819, 1922
Bme18I GGWCC 8 cut(s) 27, 245, 333, 447, 1006, 1430, 1931, 2010
BmeRI GACNNNNNGTC 1 cut(s) 91
BmgT120I GGNCC 9 cut(s) 27, 245, 333, 447, 976, 1006, 1430, 1931, 2010
BmiI GGNNCC 9 cut(s) 6, 29, 132, 247, 448, 833, 1146, 1232, 1432
BmrFI CCNGG 6 cut(s) 128, 703, 836, 1764, 1819, 1922
BmsI GCATC 9 cut(s) 786, 916, 1261, 1287, 1383, 1394, 1889, 2119, 2196
BpiI GAAGAC 4 cut(s) 183, 189, 285, 639
BpmI CTGGAG 2 cut(s) 953, 1131
BpuEI CTTGAG 4 cut(s) 738, 1304, 1322, 1899
BpuMI CCSGG 1 cut(s) 128
Bsa29I ATCGAT 1 cut(s) 255
BsaHI GRCGYC 4 cut(s) 87, 195, 365, 716
BsaI GGTCTC 1 cut(s) 1326
BsaWI WCCGGW 1 cut(s) 330
BsaXI ACNNNNNCTCC 6 cut(s) 104, 134, 538, 568, 1554, 1584
Bsc4I CCNNNNNNNGG 6 cut(s) 24, 229, 342, 456, 1763, 1818
Bse1I ACTGG 3 cut(s) 1795, 1859, 2167
Bse3DI GCAATG 2 cut(s) 1998, 2073
BseBI CCWGG 5 cut(s) 703, 836, 1764, 1819, 1922
BseCI ATCGAT 1 cut(s) 255
BseGI GGATG 7 cut(s) 69, 814, 931, 1146, 1192, 1252, 1705
BseLI CCNNNNNNNGG 6 cut(s) 24, 229, 342, 456, 1763, 1818
BseMI GCAATG 2 cut(s) 1998, 2073
BseMII CTCAG 3 cut(s) 1380, 1875, 2345
BseNI ACTGG 3 cut(s) 1795, 1859, 2167
BseRI GAGGAG 5 cut(s) 203, 355, 472, 559, 950
BseXI GCAGC 6 cut(s) 480, 642, 786, 1094, 1642, 2033
BseYI CCCAGC 1 cut(s) 1658
BsgI GTGCAG 1 cut(s) 974
Bsh1236I CGCG 2 cut(s) 349, 351
Bsh1285I CGRYCG 1 cut(s) 325
BshFI GGCC 3 cut(s) 418, 515, 978
BshNI GGYRCC 1 cut(s) 831
BshVI ATCGAT 1 cut(s) 255
BsiEI CGRYCG 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 122
BsiSI CCGG 4 cut(s) 25, 128, 331, 1978
BslFI GGGAC 4 cut(s) 460, 1416, 1690, 2199
BslI CCNNNNNNNGG 6 cut(s) 24, 229, 342, 456, 1763, 1818
BsmAI GTCTC 3 cut(s) 756, 1326, 1718
BsmFI GGGAC 4 cut(s) 460, 1416, 1690, 2199
BsmI GAATGC 1 cut(s) 1854
BsnI GGCC 3 cut(s) 418, 515, 978
Bso31I GGTCTC 1 cut(s) 1326
Bsp1286I GDGCHC 3 cut(s) 122, 1198, 1233
Bsp143I GATC 9 cut(s) 4, 9, 309, 322, 677, 732, 909, 1241, 2126
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 9 cut(s) 209, 240, 243, 291, 361, 444, 471, 783, 2309
BspANI GGCC 3 cut(s) 418, 515, 978
BspCNI CTCAG 3 cut(s) 1381, 1876, 2344
BspDI ATCGAT 1 cut(s) 255
BspFNI CGCG 2 cut(s) 349, 351
BspHI TCATGA 3 cut(s) 1272, 1333, 1690
BspLI GGNNCC 9 cut(s) 6, 29, 132, 247, 448, 833, 1146, 1232, 1432
BspMI ACCTGC 1 cut(s) 1765
BspPI GGATC 4 cut(s) 4, 12, 685, 2134
BspQI GCTCTTC 1 cut(s) 1786
BspT107I GGYRCC 1 cut(s) 831
BspTI CTTAAG 1 cut(s) 1397
BspTNI GGTCTC 1 cut(s) 1326
BsrBI CCGCTC 1 cut(s) 211
BsrDI GCAATG 2 cut(s) 1998, 2073
BsrI ACTGG 3 cut(s) 1795, 1859, 2167
BssMI GATC 9 cut(s) 4, 9, 309, 322, 677, 732, 909, 1241, 2126
BssNI GRCGYC 4 cut(s) 87, 195, 365, 716
BssSI CACGAG 1 cut(s) 337
BssT1I CCWWGG 3 cut(s) 150, 228, 847
Bst2BI CACGAG 1 cut(s) 337
Bst2UI CCWGG 5 cut(s) 703, 836, 1764, 1819, 1922
Bst4CI ACNGT 7 cut(s) 64, 451, 643, 884, 1570, 2035, 2175
Bst6I CTCTTC 6 cut(s) 267, 567, 756, 849, 1786, 2185
BstACI GRCGYC 4 cut(s) 87, 195, 365, 716
BstAFI CTTAAG 1 cut(s) 1397
BstC8I GCNNGC 7 cut(s) 110, 363, 513, 1290, 1470, 2108, 2325
BstDEI CTNAG 6 cut(s) 1389, 1884, 2178, 2331, 2369, 2381
BstDSI CCRYGG 3 cut(s) 150, 450, 2136
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 7 cut(s) 69, 814, 931, 1146, 1192, 1252, 1705
BstFNI CGCG 2 cut(s) 349, 351
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 4 cut(s) 351, 476, 1113, 1796
BstKTI GATC 9 cut(s) 7, 12, 312, 325, 680, 735, 912, 1244, 2129
BstMAI GTCTC 3 cut(s) 756, 1326, 1718
BstMBI GATC 9 cut(s) 4, 9, 309, 322, 677, 732, 909, 1241, 2126
BstMCI CGRYCG 1 cut(s) 325
BstMWI GCNNNNNNNGC 9 cut(s) 300, 664, 1469, 1484, 1493, 1800, 1944, 2002, 2141
BstNI CCWGG 5 cut(s) 703, 836, 1764, 1819, 1922
BstNSI RCATGY 1 cut(s) 2110
BstSCI CCNGG 6 cut(s) 126, 701, 834, 1762, 1817, 1920
BstSFI CTRYAG 3 cut(s) 1874, 2130, 2147
BstUI CGCG 2 cut(s) 349, 351
BstV1I GCAGC 6 cut(s) 480, 642, 786, 1094, 1642, 2033
BstV2I GAAGAC 4 cut(s) 183, 189, 285, 639
BstX2I RGATCY 4 cut(s) 4, 677, 1241, 2126
BstYI RGATCY 4 cut(s) 4, 677, 1241, 2126
Bsu15I ATCGAT 1 cut(s) 255
BsuRI GGCC 3 cut(s) 418, 515, 978
BsuTUI ATCGAT 1 cut(s) 255
BtgI CCRYGG 3 cut(s) 150, 450, 2136
BtsCI GGATG 7 cut(s) 69, 814, 931, 1146, 1192, 1252, 1705
BtsI GCAGTG 4 cut(s) 397, 504, 559, 1954
BtsIMutI CAGTG 6 cut(s) 397, 504, 559, 1254, 1954, 2040
BveI ACCTGC 1 cut(s) 1765
Cac8I GCNNGC 7 cut(s) 110, 363, 513, 1290, 1470, 2108, 2325
CciI TCATGA 3 cut(s) 1272, 1333, 1690
CfoI GCGC 4 cut(s) 351, 476, 1113, 1796
Cfr13I GGNCC 9 cut(s) 27, 245, 333, 447, 976, 1006, 1430, 1931, 2010
ClaI ATCGAT 1 cut(s) 255
CseI GACGC 3 cut(s) 95, 203, 354
Csp6I GTAC 4 cut(s) 649, 832, 880, 1527
CviAII CATG 7 cut(s) 151, 1102, 1273, 1334, 1691, 2107, 2220
CviQI GTAC 4 cut(s) 649, 832, 880, 1527
DdeI CTNAG 6 cut(s) 1389, 1884, 2178, 2331, 2369, 2381
DpnI GATC 9 cut(s) 6, 11, 311, 324, 679, 734, 911, 1243, 2128
DpnII GATC 9 cut(s) 4, 9, 309, 322, 677, 732, 909, 1241, 2126
DriI GACNNNNNGTC 1 cut(s) 91
Eam1104I CTCTTC 6 cut(s) 267, 567, 756, 849, 1786, 2185
Eam1105I GACNNNNNGTC 1 cut(s) 91
EarI CTCTTC 6 cut(s) 267, 567, 756, 849, 1786, 2185
EciI GGCGGA 1 cut(s) 258
Ecl136II GAGCTC 1 cut(s) 120
Eco130I CCWWGG 3 cut(s) 150, 228, 847
Eco147I AGGCCT 1 cut(s) 515
Eco24I GRGCYC 3 cut(s) 122, 1198, 1233
Eco31I GGTCTC 1 cut(s) 1326
Eco32I GATATC 1 cut(s) 1278
Eco47I GGWCC 8 cut(s) 27, 245, 333, 447, 1006, 1430, 1931, 2010
Eco53kI GAGCTC 1 cut(s) 120
Eco57I CTGAAG 2 cut(s) 1230, 1593
EcoICRI GAGCTC 1 cut(s) 120
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 976
EcoRII CCWGG 5 cut(s) 701, 834, 1762, 1817, 1920
EcoRV GATATC 1 cut(s) 1278
EcoT14I CCWWGG 3 cut(s) 150, 228, 847
EcoT22I ATGCAT 1 cut(s) 2112
EcoT38I GRGCYC 3 cut(s) 122, 1198, 1233
ErhI CCWWGG 3 cut(s) 150, 228, 847
FaeI CATG 7 cut(s) 154, 1105, 1276, 1337, 1694, 2110, 2223
FalI AAGNNNNNCTT 4 cut(s) 846, 878, 1989, 2021
FaqI GGGAC 4 cut(s) 460, 1416, 1690, 2199
FatI CATG 7 cut(s) 150, 1101, 1272, 1333, 1690, 2106, 2219
FauI CCCGC 2 cut(s) 354, 437
FbaI TGATCA 2 cut(s) 309, 909
FblI GTMKAC 1 cut(s) 1372
FokI GGATG 7 cut(s) 56, 801, 938, 1153, 1199, 1239, 1712
FriOI GRGCYC 3 cut(s) 122, 1198, 1233
FspBI CTAG 1 cut(s) 1719
GlaI GCGC 4 cut(s) 350, 475, 1112, 1795
GsaI CCCAGC 1 cut(s) 1662
GsuI CTGGAG 2 cut(s) 953, 1131
HaeII RGCGCY 1 cut(s) 477
HaeIII GGCC 3 cut(s) 418, 515, 978
HapII CCGG 4 cut(s) 25, 128, 331, 1978
HgaI GACGC 3 cut(s) 95, 203, 354
HhaI GCGC 4 cut(s) 351, 476, 1113, 1796
Hin1I GRCGYC 4 cut(s) 87, 195, 365, 716
Hin1II CATG 7 cut(s) 154, 1105, 1276, 1337, 1694, 2110, 2223
Hin6I GCGC 4 cut(s) 349, 474, 1111, 1794
HinP1I GCGC 4 cut(s) 349, 474, 1111, 1794
HincII GTYRAC 1 cut(s) 1041
HindII GTYRAC 1 cut(s) 1041
HindIII AAGCTT 3 cut(s) 966, 1983, 2003
HinfI GANTC 3 cut(s) 92, 434, 2260
HpaI GTTAAC 1 cut(s) 1041
HpaII CCGG 4 cut(s) 25, 128, 331, 1978
HphI GGTGA 7 cut(s) 7, 424, 466, 1079, 1655, 1746, 2177
Hpy166II GTNNAC 4 cut(s) 1009, 1041, 1373, 2227
Hpy188I TCNGA 8 cut(s) 9, 124, 523, 721, 819, 1390, 1885, 2089
Hpy8I GTNNAC 4 cut(s) 1009, 1041, 1373, 2227
HpyAV CCTTC 7 cut(s) 380, 667, 967, 1584, 1658, 1761, 2176
HpyCH4III ACNGT 7 cut(s) 64, 451, 643, 884, 1570, 2035, 2175
HpyCH4IV ACGT 3 cut(s) 716, 1118, 1679
HpyF10VI GCNNNNNNNGC 9 cut(s) 300, 664, 1469, 1484, 1493, 1800, 1944, 2002, 2141
HpyF3I CTNAG 6 cut(s) 1389, 1884, 2178, 2331, 2369, 2381
HpySE526I ACGT 3 cut(s) 716, 1118, 1679
Hsp92I GRCGYC 4 cut(s) 87, 195, 365, 716
Hsp92II CATG 7 cut(s) 154, 1105, 1276, 1337, 1694, 2110, 2223
HspAI GCGC 4 cut(s) 349, 474, 1111, 1794
KpnI GGTACC 1 cut(s) 835
Ksp22I TGATCA 2 cut(s) 309, 909
KspAI GTTAAC 1 cut(s) 1041
Kzo9I GATC 9 cut(s) 4, 9, 309, 322, 677, 732, 909, 1241, 2126
LguI GCTCTTC 1 cut(s) 1786
Lsp1109I GCAGC 6 cut(s) 480, 642, 786, 1094, 1642, 2033
LweI GCATC 9 cut(s) 786, 916, 1261, 1287, 1383, 1394, 1889, 2119, 2196
MaeI CTAG 1 cut(s) 1719
MaeII ACGT 3 cut(s) 716, 1118, 1679
MaeIII GTNAC 4 cut(s) 809, 1667, 1861, 2294
MalI GATC 9 cut(s) 6, 11, 311, 324, 679, 734, 911, 1243, 2128
MbiI CCGCTC 1 cut(s) 211
MboI GATC 9 cut(s) 4, 9, 309, 322, 677, 732, 909, 1241, 2126
MfeI CAATTG 2 cut(s) 1212, 1530
MflI RGATCY 4 cut(s) 4, 677, 1241, 2126
MhlI GDGCHC 3 cut(s) 122, 1198, 1233
MluCI AATT 5 cut(s) 777, 1212, 1530, 1805, 1828
MlyI GAGTC 2 cut(s) 101, 443
MmeI TCCRAC 4 cut(s) 147, 501, 699, 1906
Mph1103I ATGCAT 1 cut(s) 2112
MroXI GAANNNNTTC 1 cut(s) 1155
MseI TTAA 5 cut(s) 315, 711, 1020, 1040, 1398
MslI CAYNNNNRTG 3 cut(s) 78, 593, 819
MspA1I CMGCKG 3 cut(s) 444, 471, 2024
MspCI CTTAAG 1 cut(s) 1397
MspI CCGG 4 cut(s) 25, 128, 331, 1978
MspR9I CCNGG 6 cut(s) 128, 703, 836, 1764, 1819, 1922
MunI CAATTG 2 cut(s) 1212, 1530
Mva1269I GAATGC 1 cut(s) 1854
MvaI CCWGG 5 cut(s) 703, 836, 1764, 1819, 1922
MvnI CGCG 2 cut(s) 349, 351
MwoI GCNNNNNNNGC 9 cut(s) 300, 664, 1469, 1484, 1493, 1800, 1944, 2002, 2141
NciI CCSGG 1 cut(s) 128
NcoI CCATGG 1 cut(s) 150
NdeII GATC 9 cut(s) 4, 9, 309, 322, 677, 732, 909, 1241, 2126
NlaIII CATG 7 cut(s) 154, 1105, 1276, 1337, 1694, 2110, 2223
NlaIV GGNNCC 9 cut(s) 6, 29, 132, 247, 448, 833, 1146, 1232, 1432
NmeAIII GCCGAG 1 cut(s) 115
NsiI ATGCAT 1 cut(s) 2112
NspI RCATGY 1 cut(s) 2110
PaeI GCATGC 1 cut(s) 2110
PagI TCATGA 3 cut(s) 1272, 1333, 1690
PaqCI CACCTGC 1 cut(s) 1765
PasI CCCWGGG 1 cut(s) 1818
PceI AGGCCT 1 cut(s) 515
PciSI GCTCTTC 1 cut(s) 1786
PcsI WCGNNNNNNNCGW 4 cut(s) 28, 186, 261, 605
PctI GAATGC 1 cut(s) 1854
PdmI GAANNNNTTC 1 cut(s) 1155
PfeI GAWTC 1 cut(s) 2260
PfoI TCCNGGA 1 cut(s) 1762
Ple19I CGATCG 1 cut(s) 325
PleI GAGTC 2 cut(s) 100, 442
PpsI GAGTC 2 cut(s) 100, 442
PsiI TTATAA 1 cut(s) 696
Psp124BI GAGCTC 1 cut(s) 122
Psp6I CCWGG 5 cut(s) 701, 834, 1762, 1817, 1920
PspFI CCCAGC 1 cut(s) 1658
PspGI CCWGG 5 cut(s) 701, 834, 1762, 1817, 1920
PspN4I GGNNCC 9 cut(s) 6, 29, 132, 247, 448, 833, 1146, 1232, 1432
PspPI GGNCC 9 cut(s) 27, 245, 333, 447, 976, 1006, 1430, 1931, 2010
PsrI GAACNNNNNNTAC 2 cut(s) 1356, 1388
PsuI RGATCY 4 cut(s) 4, 677, 1241, 2126
PvuI CGATCG 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 2024
RsaI GTAC 4 cut(s) 650, 833, 881, 1528
RsaNI GTAC 4 cut(s) 649, 832, 880, 1527
RseI CAYNNNNRTG 3 cut(s) 78, 593, 819
SacI GAGCTC 1 cut(s) 122
SapI GCTCTTC 1 cut(s) 1786
SaqAI TTAA 5 cut(s) 315, 711, 1020, 1040, 1398
Sau3AI GATC 9 cut(s) 4, 9, 309, 322, 677, 732, 909, 1241, 2126
Sau96I GGNCC 9 cut(s) 27, 245, 333, 447, 976, 1006, 1430, 1931, 2010
ScaI AGTACT 1 cut(s) 881
SchI GAGTC 2 cut(s) 101, 443
ScrFI CCNGG 6 cut(s) 128, 703, 836, 1764, 1819, 1922
SduI GDGCHC 3 cut(s) 122, 1198, 1233
SfaNI GCATC 9 cut(s) 786, 916, 1261, 1287, 1383, 1394, 1889, 2119, 2196
SfcI CTRYAG 3 cut(s) 1874, 2130, 2147
SinI GGWCC 8 cut(s) 27, 245, 333, 447, 1006, 1430, 1931, 2010
SmiMI CAYNNNNRTG 3 cut(s) 78, 593, 819
SmlI CTYRAG 5 cut(s) 753, 1283, 1301, 1397, 1914
SmoI CTYRAG 5 cut(s) 753, 1283, 1301, 1397, 1914
SphI GCATGC 1 cut(s) 2110
Sse9I AATT 5 cut(s) 777, 1212, 1530, 1805, 1828
SseBI AGGCCT 1 cut(s) 515
SsiI CCGC 9 cut(s) 209, 240, 243, 291, 361, 444, 471, 783, 2309
SspI AATATT 1 cut(s) 1033
SspMI CTAG 1 cut(s) 1719
SstI GAGCTC 1 cut(s) 122
StuI AGGCCT 1 cut(s) 515
StyD4I CCNGG 6 cut(s) 126, 701, 834, 1762, 1817, 1920
StyI CCWWGG 3 cut(s) 150, 228, 847
TaaI ACNGT 7 cut(s) 64, 451, 643, 884, 1570, 2035, 2175
TaiI ACGT 3 cut(s) 719, 1121, 1682
TaqII GACCGA 1 cut(s) 427
TasI AATT 5 cut(s) 777, 1212, 1530, 1805, 1828
TatI WGTACW 2 cut(s) 879, 1526
TauI GCSGC 4 cut(s) 243, 294, 474, 2311
TfiI GAWTC 1 cut(s) 2260
Tru1I TTAA 5 cut(s) 315, 711, 1020, 1040, 1398
Tru9I TTAA 5 cut(s) 315, 711, 1020, 1040, 1398
TscAI CASTG 6 cut(s) 397, 504, 559, 1254, 1954, 2040
TseI GCWGC 6 cut(s) 468, 655, 799, 1082, 1630, 2021
TspGWI ACGGA 3 cut(s) 396, 1191, 1426
TspRI CASTG 6 cut(s) 397, 504, 559, 1254, 1954, 2040
Vha464I CTTAAG 1 cut(s) 1397
VpaK11BI GGWCC 8 cut(s) 27, 245, 333, 447, 1006, 1430, 1931, 2010
XagI CCTNNNNNAGG 1 cut(s) 227
XapI RAATTY 2 cut(s) 777, 1828
XceI RCATGY 1 cut(s) 2110
XmiI GTMKAC 1 cut(s) 1372
XmnI GAANNNNTTC 1 cut(s) 1155
XspI CTAG 1 cut(s) 1719
ZraI GACGTC 1 cut(s) 717
ZrmI AGTACT 1 cut(s) 881
Zsp2I ATGCAT 1 cut(s) 2112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.