pycom16g05200

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009465
Physical Location & Seq
Reverse (-)
3387426 .. 3393881
6456 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g05200.15

Sequence Viewer

Length: 2340 bp
ATGGATCCGATCCTCACCACTCGCCGGACCCGAAAAATCCCACCCAAACCCGATTACTCAACGGTAGTCATCCACGACGACGATGACTCCGAGTCCGATCGACAGAGCAAGCACAAGAGCTCTGACCTGGAACCCGACCTGTACGCCACCATGGTCTATAAGGGTAATGCCGACGAAGACGAAGACGAAGACGACGACGCCTCTCTCCCTCCGCTCCTCAAACGCCTCCCGAAGGACTTCGGCGGCGGACCCTCAATCGATTACTTTGACGACGAAGAGGAAGACGACAACGGCGGCGATTTCGGCACAATGATCATCAAGCCCGATCGAAACCGGACCACGAGGAGGTCGCGCGACTTCAAGCGGGCGTCGTTCGACGATGACGGAGACGGCAGTGGGTTCTCGACATTTGTGGTGAGGCCGTCGAGTGAGAGAGAGTCGGTCAGCGGGACCGTGGTGAGGAGGACGAGCAGTGGCGCTGGTTCGACAATGAGTAGGGCAGTGGCGAGTATGCAGGCCTCGTCGGAGTTAGGAGTTGGGAAGCAGAGGAGGGGCAGTGGGTCGTCGCACGGAGAAGAGTATCGGCAAACCACTAAAATGTCGTCGAGTTCGATACCAGATAGCGTTACCAGAGAAGATCCAACTGTAAAGTACGAGCTGCTCAATGAGCTTGGGAAGGGGTCGTATGGCGCTGTTTATAAAGCTAGGGATATTAAGACGTCGGAGCTGGTGGCCATCAAAGTCATTTCATTGTCTCAAGGGGAAGAGGGGTATGAAGAAATTTGCGGAGAAATCGAGATGCTGCAACAATGTAACCATCCGAATGTGGTTCGGTACCTGGGAAGCTACCAAGGAGAAGAGTATCTTTGGATTGTGATGGAGTACTGTGGTGGTGGTAGTATTGCCGACTTGATCAATGCTACCGAGGATGCTCTGGAGGAGTATCAAATAGCCTACATTTGTAGGGAAGCGTTGAAGGGCCTTGCTTATCTGCACTCAATTTTCAAGGTCCACAGAGATATTAAGGGTGGTAATATTCTGTTAACTGAACAAGGAGATGTCAAGTTGGGTGATTTTGGTGTTGCAGCTCAACTTACAAGAACCATGTCAAAGCGCAACACGTTCATTGGCACTCCACATTGGATGGCTCCAGAAGTTATTCAGGAAAGTCGCTATGATGGAAAGGTGGATGTATGGGCTCTTGGTGTTTCTGCAATTGAAATGGCAGAGGGGCTCCCTCCAAGATCTTCAGTGCATCCAATGAGGGTTTTATTCATGATATCCCTTGAGCCAGCCCCGATGCTTGAGGATAAAGAAAAATGGTCTCTTGTGTTTCATGATTTTGTGGCAAAGTGCCTAACTAAAGAACCTCGTCTACGCCCTACTGCATCTGAGATGCTGAAGCACAAGTTCATTGAAAAATGCCAATGTGGTCCCTCTGTAATGTTGACAAAGATTGAAAAGGCAAGGCAAGCAAGGGCTTCAATGGCTTTGCAAGCACAACATATTGCTCCACCTGAACAGGATAGTACAATTGAAGCTCCCAAAGTCAATGAGGATTATGGAGATACTGTTCCTTCAAGGCCACTTCAAGTGGAAAATGAAGTTTCTTCAGGTAACTTTGGCACCTTTATTATTCACGAGGGGGATGAAAAAGATGAGATCTCTAGTGAGACAAAGAGTTTTAGTGTCAAAGAACCTTCACCTGCTCCTGGACTTCCTGGAAGCGCCTCCAATGCTGATGCAGGAGGCAAATTACCCGAACCCAGGGAGGAAAATTCAAGTAGTATTACCAAAAACAGCATTCCAGTTACGCACACTCTACAAGCATCTGAGCAGAGCATAAAGACAAAAGGCATATCTCAAGTCCAGGTTGGAGATCCAAGCGGTATAAGCAGTGGCACAGTGAAGAATGAAACTGTCAGCCGGAAGGCTTTCGCAATGCAAGATAAGCTATGGTCCATATATGCAGCTGGTAATACAGTGCCTATTCCATTTTTGAGGGCAACCGATATATCCCCCATTGCTCTTTTATCAGACACTGTTCTTGGAAGCATTCATCAGGATAATAGTGGAACTGTAGCCATGGAGGCACTACAAGAGCTTTTTACTGGTGATGGACAGTCTAAGAAGGGACGAAGAGCGCAAAACGAGATTCCCCTCCCTCCAAGTGTTTACAAAAGGCTCTTTACAAGTTCAACTCTAATGAATCTGGCACAAGCATTAGCCTACCACAAGATGTGCTATGAAGATATGCCGCTTCAAGAAATGCAAGCAACTCAGGAGCAACAGACTATTCAAAATCTTTGTGATACACTAAGAACCATTCTTCGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006950 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007266 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0009893 GO:0009966 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0012501 GO:0016043 GO:0016301 GO:0016310 GO:0016477 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017048 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023051 GO:0023052 GO:0030029 GO:0030036 GO:0031098 GO:0031267 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0040011 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043408 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048365 GO:0048518 GO:0048522 GO:0048583 GO:0048870 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051674 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902531
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

780

Amino Acids

85.82

Weight (kDa)

5.11

Isoelectric Point (pI)

54.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 699
AarI CACCTGC 1 cut(s) 1714
AatII GACGTC 1 cut(s) 722
Acc36I ACCTGC 1 cut(s) 1714
Acc65I GGTACC 1 cut(s) 834
AccB1I GGYRCC 2 cut(s) 834, 1625
AccBSI CCGCTC 1 cut(s) 214
AccI GTMKAC 1 cut(s) 1375
AccII CGCG 2 cut(s) 352, 354
AciI CCGC 9 cut(s) 212, 243, 246, 294, 364, 447, 786, 1887, 2258
AclWI GGATC 4 cut(s) 4, 12, 632, 1874
AcoI YGGCCR 1 cut(s) 732
AcsI RAATTY 2 cut(s) 780, 1777
AcuI CTGAAG 3 cut(s) 1233, 1421, 1596
AcyI GRCGYC 3 cut(s) 198, 368, 719
AfaI GTAC 5 cut(s) 143, 653, 836, 884, 1531
AfiI CCNNNNNNNGG 6 cut(s) 24, 232, 345, 459, 1712, 1767
AflIII ACRYGT 1 cut(s) 1119
AhdI GACNNNNNGTC 1 cut(s) 91
AjnI CCWGG 6 cut(s) 126, 837, 1711, 1720, 1766, 1869
AloI GAACNNNNNNTCC 2 cut(s) 1557, 1589
Alw21I GWGCWC 1 cut(s) 122
Alw26I GTCTC 4 cut(s) 381, 759, 1329, 1667
AlwI GGATC 4 cut(s) 4, 12, 632, 1874
AlwNI CAGNNNCTG 1 cut(s) 2042
AoxI GGCC 5 cut(s) 419, 516, 732, 979, 1583
ApeKI GCWGC 4 cut(s) 658, 802, 1085, 1970
ApoI RAATTY 2 cut(s) 780, 1777
Asp700I GAANNNNTTC 3 cut(s) 236, 1158, 1934
Asp718I GGTACC 1 cut(s) 834
AspLEI GCGC 6 cut(s) 354, 479, 692, 1116, 1730, 2146
AspS9I GGNCC 8 cut(s) 27, 248, 336, 450, 979, 1009, 1433, 1959
AsuHPI GGTGA 6 cut(s) 7, 427, 469, 1082, 1695, 2126
AvaII GGWCC 7 cut(s) 27, 248, 336, 450, 1009, 1433, 1959
BalI TGGCCA 1 cut(s) 734
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 834, 1625
BanII GRGCYC 3 cut(s) 122, 1201, 1236
BauI CACGAG 2 cut(s) 340, 1640
BbsI GAAGAC 4 cut(s) 183, 189, 195, 288
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 4 cut(s) 645, 789, 1097, 1982
BccI CCATC 6 cut(s) 743, 825, 871, 1138, 1172, 2111
BceAI ACGGC 3 cut(s) 307, 406, 406
BciT130I CCWGG 6 cut(s) 128, 839, 1713, 1722, 1768, 1871
BclI TGATCA 2 cut(s) 312, 912
BcoDI GTCTC 4 cut(s) 381, 759, 1329, 1667
BfaI CTAG 2 cut(s) 705, 1668
BfmI CTRYAG 1 cut(s) 2079
BfoI RGCGCY 3 cut(s) 480, 693, 1731
BfuAI ACCTGC 1 cut(s) 1714
BglI GCCNNNNNGGC 1 cut(s) 2090
BglII AGATCT 2 cut(s) 1244, 1662
BisI GCNGC 7 cut(s) 244, 295, 659, 803, 1086, 1971, 2258
BlsI GCNGC 7 cut(s) 245, 296, 660, 804, 1087, 1972, 2259
BmcAI AGTACT 1 cut(s) 884
Bme1390I CCNGG 6 cut(s) 128, 839, 1713, 1722, 1768, 1871
Bme18I GGWCC 7 cut(s) 27, 248, 336, 450, 1009, 1433, 1959
BmeRI GACNNNNNGTC 1 cut(s) 91
BmgT120I GGNCC 8 cut(s) 27, 248, 336, 450, 979, 1009, 1433, 1959
BmrFI CCNGG 6 cut(s) 128, 839, 1713, 1722, 1768, 1871
BmsI GCATC 8 cut(s) 789, 919, 1264, 1290, 1386, 1397, 1732, 1838
BpiI GAAGAC 4 cut(s) 183, 189, 195, 288
BpmI CTGGAG 2 cut(s) 956, 1134
BpuEI CTTGAG 4 cut(s) 741, 1307, 1325, 1848
Bsa29I ATCGAT 1 cut(s) 258
BsaHI GRCGYC 3 cut(s) 198, 368, 719
BsaI GGTCTC 1 cut(s) 1329
BsaJI CCNNGG 8 cut(s) 150, 453, 838, 850, 924, 1766, 1767, 2085
BsaWI WCCGGW 1 cut(s) 333
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 541, 571, 1557, 1587
Bsc4I CCNNNNNNNGG 6 cut(s) 24, 232, 345, 459, 1712, 1767
Bse1I ACTGG 2 cut(s) 1808, 2116
Bse3DI GCAATG 2 cut(s) 1947, 2022
BseBI CCWGG 6 cut(s) 128, 839, 1713, 1722, 1768, 1871
BseCI ATCGAT 1 cut(s) 258
BseDI CCNNGG 8 cut(s) 150, 453, 838, 850, 924, 1766, 1767, 2085
BseGI GGATG 7 cut(s) 69, 817, 934, 1149, 1195, 1255, 1654
BseLI CCNNNNNNNGG 6 cut(s) 24, 232, 345, 459, 1712, 1767
BseMI GCAATG 2 cut(s) 1947, 2022
BseMII CTCAG 3 cut(s) 1383, 1824, 2294
BseNI ACTGG 2 cut(s) 1808, 2116
BseRI GAGGAG 5 cut(s) 206, 358, 475, 562, 953
BseXI GCAGC 4 cut(s) 645, 789, 1097, 1982
BsgI GTGCAG 1 cut(s) 977
Bsh1236I CGCG 2 cut(s) 352, 354
Bsh1285I CGRYCG 2 cut(s) 100, 328
BshFI GGCC 5 cut(s) 421, 518, 734, 981, 1585
BshNI GGYRCC 2 cut(s) 834, 1625
BshVI ATCGAT 1 cut(s) 258
BsiEI CGRYCG 2 cut(s) 100, 328
BsiHKAI GWGCWC 1 cut(s) 122
BsiSI CCGG 3 cut(s) 25, 334, 1927
BslFI GGGAC 3 cut(s) 463, 1419, 2148
BslI CCNNNNNNNGG 6 cut(s) 24, 232, 345, 459, 1712, 1767
BsmAI GTCTC 4 cut(s) 381, 759, 1329, 1667
BsmBI CGTCTC 1 cut(s) 381
BsmFI GGGAC 3 cut(s) 463, 1419, 2148
BsmI GAATGC 2 cut(s) 1803, 2055
BsnI GGCC 5 cut(s) 421, 518, 734, 981, 1585
Bso31I GGTCTC 1 cut(s) 1329
Bsp1286I GDGCHC 3 cut(s) 122, 1201, 1236
Bsp19I CCATGG 2 cut(s) 150, 2085
BspACI CCGC 9 cut(s) 212, 243, 246, 294, 364, 447, 786, 1887, 2258
BspANI GGCC 5 cut(s) 421, 518, 734, 981, 1585
BspCNI CTCAG 3 cut(s) 1384, 1825, 2293
BspDI ATCGAT 1 cut(s) 258
BspFNI CGCG 2 cut(s) 352, 354
BspHI TCATGA 2 cut(s) 1275, 1336
BspMI ACCTGC 1 cut(s) 1714
BspPI GGATC 4 cut(s) 4, 12, 632, 1874
BspQI GCTCTTC 1 cut(s) 2134
BspT107I GGYRCC 2 cut(s) 834, 1625
BspTNI GGTCTC 1 cut(s) 1329
BsrBI CCGCTC 1 cut(s) 214
BsrDI GCAATG 2 cut(s) 1947, 2022
BsrI ACTGG 2 cut(s) 1808, 2116
BssECI CCNNGG 8 cut(s) 150, 453, 838, 850, 924, 1766, 1767, 2085
BssNI GRCGYC 3 cut(s) 198, 368, 719
BssSI CACGAG 2 cut(s) 340, 1640
BssT1I CCWWGG 3 cut(s) 150, 850, 2085
Bst2BI CACGAG 2 cut(s) 340, 1640
Bst2UI CCWGG 6 cut(s) 128, 839, 1713, 1722, 1768, 1871
Bst6I CTCTTC 5 cut(s) 270, 570, 759, 852, 2134
BstACI GRCGYC 3 cut(s) 198, 368, 719
BstC8I GCNNGC 7 cut(s) 110, 366, 516, 1293, 1473, 1497, 2274
BstDEI CTNAG 5 cut(s) 1392, 1833, 2127, 2280, 2318
BstDSI CCRYGG 3 cut(s) 150, 453, 2085
BstENI CCTNNNNNAGG 1 cut(s) 230
BstF5I GGATG 7 cut(s) 69, 817, 934, 1149, 1195, 1255, 1654
BstFNI CGCG 2 cut(s) 352, 354
BstH2I RGCGCY 3 cut(s) 480, 693, 1731
BstHHI GCGC 6 cut(s) 354, 479, 692, 1116, 1730, 2146
BstMAI GTCTC 4 cut(s) 381, 759, 1329, 1667
BstMCI CGRYCG 2 cut(s) 100, 328
BstMWI GCNNNNNNNGC 9 cut(s) 303, 667, 1472, 1487, 1496, 1736, 1893, 1951, 2090
BstNI CCWGG 6 cut(s) 128, 839, 1713, 1722, 1768, 1871
BstSCI CCNGG 6 cut(s) 126, 837, 1711, 1720, 1766, 1869
BstSFI CTRYAG 1 cut(s) 2079
BstUI CGCG 2 cut(s) 352, 354
BstV1I GCAGC 4 cut(s) 645, 789, 1097, 1982
BstV2I GAAGAC 4 cut(s) 183, 189, 195, 288
BstX2I RGATCY 5 cut(s) 4, 637, 1244, 1662, 1879
BstYI RGATCY 5 cut(s) 4, 637, 1244, 1662, 1879
Bsu15I ATCGAT 1 cut(s) 258
BsuRI GGCC 5 cut(s) 421, 518, 734, 981, 1585
BsuTUI ATCGAT 1 cut(s) 258
BtgI CCRYGG 3 cut(s) 150, 453, 2085
BtsCI GGATG 7 cut(s) 69, 817, 934, 1149, 1195, 1255, 1654
BtsI GCAGTG 5 cut(s) 400, 478, 507, 562, 1903
BtsIMutI CAGTG 9 cut(s) 400, 478, 507, 562, 1257, 1903, 1911, 1989, 2040
BveI ACCTGC 1 cut(s) 1714
Cac8I GCNNGC 7 cut(s) 110, 366, 516, 1293, 1473, 1497, 2274
CaiI CAGNNNCTG 1 cut(s) 2042
CciI TCATGA 2 cut(s) 1275, 1336
CfoI GCGC 6 cut(s) 354, 479, 692, 1116, 1730, 2146
Cfr13I GGNCC 8 cut(s) 27, 248, 336, 450, 979, 1009, 1433, 1959
ClaI ATCGAT 1 cut(s) 258
CseI GACGC 2 cut(s) 206, 357
Csp6I GTAC 5 cut(s) 142, 652, 835, 883, 1530
CviAII CATG 5 cut(s) 151, 1105, 1276, 1337, 2086
CviQI GTAC 5 cut(s) 142, 652, 835, 883, 1530
DdeI CTNAG 5 cut(s) 1392, 1833, 2127, 2280, 2318
DriI GACNNNNNGTC 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 732
Eam1104I CTCTTC 5 cut(s) 270, 570, 759, 852, 2134
Eam1105I GACNNNNNGTC 1 cut(s) 91
EarI CTCTTC 5 cut(s) 270, 570, 759, 852, 2134
EciI GGCGGA 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 120
Eco130I CCWWGG 3 cut(s) 150, 850, 2085
Eco147I AGGCCT 1 cut(s) 518
Eco24I GRGCYC 3 cut(s) 122, 1201, 1236
Eco31I GGTCTC 1 cut(s) 1329
Eco32I GATATC 1 cut(s) 1281
Eco47I GGWCC 7 cut(s) 27, 248, 336, 450, 1009, 1433, 1959
Eco53kI GAGCTC 1 cut(s) 120
Eco57I CTGAAG 3 cut(s) 1233, 1421, 1596
EcoICRI GAGCTC 1 cut(s) 120
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 979
EcoRII CCWGG 6 cut(s) 126, 837, 1711, 1720, 1766, 1869
EcoRV GATATC 1 cut(s) 1281
EcoT14I CCWWGG 3 cut(s) 150, 850, 2085
EcoT38I GRGCYC 3 cut(s) 122, 1201, 1236
ErhI CCWWGG 3 cut(s) 150, 850, 2085
Esp3I CGTCTC 1 cut(s) 381
FaeI CATG 5 cut(s) 154, 1108, 1279, 1340, 2089
FalI AAGNNNNNCTT 2 cut(s) 849, 881
FaqI GGGAC 3 cut(s) 463, 1419, 2148
FatI CATG 5 cut(s) 150, 1104, 1275, 1336, 2085
FauI CCCGC 2 cut(s) 357, 440
FbaI TGATCA 2 cut(s) 312, 912
FblI GTMKAC 1 cut(s) 1375
Fnu4HI GCNGC 7 cut(s) 244, 295, 659, 803, 1086, 1971, 2258
FokI GGATG 7 cut(s) 56, 804, 941, 1156, 1202, 1242, 1661
FriOI GRGCYC 3 cut(s) 122, 1201, 1236
Fsp4HI GCNGC 7 cut(s) 244, 295, 659, 803, 1086, 1971, 2258
FspBI CTAG 2 cut(s) 705, 1668
GlaI GCGC 6 cut(s) 353, 478, 691, 1115, 1729, 2145
GluI GCNGC 7 cut(s) 244, 295, 659, 803, 1086, 1971, 2258
GsuI CTGGAG 2 cut(s) 956, 1134
HaeII RGCGCY 3 cut(s) 480, 693, 1731
HaeIII GGCC 5 cut(s) 421, 518, 734, 981, 1585
HapII CCGG 3 cut(s) 25, 334, 1927
HgaI GACGC 2 cut(s) 206, 357
HhaI GCGC 6 cut(s) 354, 479, 692, 1116, 1730, 2146
Hin1I GRCGYC 3 cut(s) 198, 368, 719
Hin1II CATG 5 cut(s) 154, 1108, 1279, 1340, 2089
Hin6I GCGC 6 cut(s) 352, 477, 690, 1114, 1728, 2144
HinP1I GCGC 6 cut(s) 352, 477, 690, 1114, 1728, 2144
HincII GTYRAC 2 cut(s) 1044, 1449
HindII GTYRAC 2 cut(s) 1044, 1449
HinfI GANTC 5 cut(s) 86, 92, 437, 2155, 2209
HpaI GTTAAC 1 cut(s) 1044
HpaII CCGG 3 cut(s) 25, 334, 1927
HphI GGTGA 6 cut(s) 7, 427, 469, 1082, 1695, 2126
Hpy166II GTNNAC 5 cut(s) 1012, 1044, 1376, 1449, 2176
Hpy8I GTNNAC 5 cut(s) 1012, 1044, 1376, 1449, 2176
HpyAV CCTTC 7 cut(s) 226, 670, 970, 1587, 1710, 1924, 2125
HpyCH4IV ACGT 2 cut(s) 719, 1121
HpyF10VI GCNNNNNNNGC 9 cut(s) 303, 667, 1472, 1487, 1496, 1736, 1893, 1951, 2090
HpyF3I CTNAG 5 cut(s) 1392, 1833, 2127, 2280, 2318
HpySE526I ACGT 2 cut(s) 719, 1121
Hsp92I GRCGYC 3 cut(s) 198, 368, 719
Hsp92II CATG 5 cut(s) 154, 1108, 1279, 1340, 2089
HspAI GCGC 6 cut(s) 352, 477, 690, 1114, 1728, 2144
KpnI GGTACC 1 cut(s) 838
Ksp22I TGATCA 2 cut(s) 312, 912
KspAI GTTAAC 1 cut(s) 1044
LguI GCTCTTC 1 cut(s) 2134
LmnI GCTCC 8 cut(s) 219, 724, 1153, 1239, 1516, 1546, 1714, 2284
Lsp1109I GCAGC 4 cut(s) 645, 789, 1097, 1982
LweI GCATC 8 cut(s) 789, 919, 1264, 1290, 1386, 1397, 1732, 1838
MaeI CTAG 2 cut(s) 705, 1668
MaeII ACGT 2 cut(s) 719, 1121
MaeIII GTNAC 4 cut(s) 625, 812, 1616, 1810
MbiI CCGCTC 1 cut(s) 214
MfeI CAATTG 2 cut(s) 1215, 1533
MflI RGATCY 5 cut(s) 4, 637, 1244, 1662, 1879
MhlI GDGCHC 3 cut(s) 122, 1201, 1236
MlsI TGGCCA 1 cut(s) 734
MluCI AATT 6 cut(s) 780, 999, 1215, 1533, 1754, 1777
MluNI TGGCCA 1 cut(s) 734
MlyI GAGTC 3 cut(s) 80, 101, 446
MmeI TCCRAC 4 cut(s) 504, 665, 702, 1855
Mox20I TGGCCA 1 cut(s) 734
MroXI GAANNNNTTC 3 cut(s) 236, 1158, 1934
MscI TGGCCA 1 cut(s) 734
MseI TTAA 3 cut(s) 714, 1023, 1043
MslI CAYNNNNRTG 2 cut(s) 596, 822
Msp20I TGGCCA 1 cut(s) 734
MspA1I CMGCKG 2 cut(s) 447, 1973
MspI CCGG 3 cut(s) 25, 334, 1927
MspR9I CCNGG 6 cut(s) 128, 839, 1713, 1722, 1768, 1871
MunI CAATTG 2 cut(s) 1215, 1533
Mva1269I GAATGC 2 cut(s) 1803, 2055
MvaI CCWGG 6 cut(s) 128, 839, 1713, 1722, 1768, 1871
MvnI CGCG 2 cut(s) 352, 354
MwoI GCNNNNNNNGC 9 cut(s) 303, 667, 1472, 1487, 1496, 1736, 1893, 1951, 2090
NcoI CCATGG 2 cut(s) 150, 2085
NlaIII CATG 5 cut(s) 154, 1108, 1279, 1340, 2089
PagI TCATGA 2 cut(s) 1275, 1336
PaqCI CACCTGC 1 cut(s) 1714
PasI CCCWGGG 1 cut(s) 1767
PceI AGGCCT 1 cut(s) 518
PciSI GCTCTTC 1 cut(s) 2134
PcsI WCGNNNNNNNCGW 4 cut(s) 28, 87, 192, 608
PctI GAATGC 2 cut(s) 1803, 2055
PdmI GAANNNNTTC 3 cut(s) 236, 1158, 1934
PfeI GAWTC 2 cut(s) 2155, 2209
PfoI TCCNGGA 2 cut(s) 1711, 1720
PkrI GCNGC 7 cut(s) 245, 296, 660, 804, 1087, 1972, 2259
Ple19I CGATCG 2 cut(s) 100, 328
PleI GAGTC 3 cut(s) 80, 100, 445
PpsI GAGTC 3 cut(s) 80, 100, 445
PsiI TTATAA 1 cut(s) 699
Psp124BI GAGCTC 1 cut(s) 122
Psp6I CCWGG 6 cut(s) 126, 837, 1711, 1720, 1766, 1869
PspGI CCWGG 6 cut(s) 126, 837, 1711, 1720, 1766, 1869
PspPI GGNCC 8 cut(s) 27, 248, 336, 450, 979, 1009, 1433, 1959
PsrI GAACNNNNNNTAC 2 cut(s) 1359, 1391
PstNI CAGNNNCTG 1 cut(s) 2042
PsuI RGATCY 5 cut(s) 4, 637, 1244, 1662, 1879
PvuI CGATCG 2 cut(s) 100, 328
PvuII CAGCTG 1 cut(s) 1973
RsaI GTAC 5 cut(s) 143, 653, 836, 884, 1531
RsaNI GTAC 5 cut(s) 142, 652, 835, 883, 1530
RseI CAYNNNNRTG 2 cut(s) 596, 822
SacI GAGCTC 1 cut(s) 122
SapI GCTCTTC 1 cut(s) 2134
SaqAI TTAA 3 cut(s) 714, 1023, 1043
SatI GCNGC 7 cut(s) 244, 295, 659, 803, 1086, 1971, 2258
Sau96I GGNCC 8 cut(s) 27, 248, 336, 450, 979, 1009, 1433, 1959
ScaI AGTACT 1 cut(s) 884
SchI GAGTC 3 cut(s) 80, 101, 446
ScrFI CCNGG 6 cut(s) 128, 839, 1713, 1722, 1768, 1871
SduI GDGCHC 3 cut(s) 122, 1201, 1236
SfaNI GCATC 8 cut(s) 789, 919, 1264, 1290, 1386, 1397, 1732, 1838
SfcI CTRYAG 1 cut(s) 2079
SinI GGWCC 7 cut(s) 27, 248, 336, 450, 1009, 1433, 1959
SmiMI CAYNNNNRTG 2 cut(s) 596, 822
SmlI CTYRAG 4 cut(s) 756, 1286, 1304, 1863
SmoI CTYRAG 4 cut(s) 756, 1286, 1304, 1863
Sse9I AATT 6 cut(s) 780, 999, 1215, 1533, 1754, 1777
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 9 cut(s) 212, 243, 246, 294, 364, 447, 786, 1887, 2258
SspI AATATT 1 cut(s) 1036
SspMI CTAG 2 cut(s) 705, 1668
SstI GAGCTC 1 cut(s) 122
StuI AGGCCT 1 cut(s) 518
StyD4I CCNGG 6 cut(s) 126, 837, 1711, 1720, 1766, 1869
StyI CCWWGG 3 cut(s) 150, 850, 2085
TaiI ACGT 2 cut(s) 722, 1124
TaqII GACCGA 1 cut(s) 430
TasI AATT 6 cut(s) 780, 999, 1215, 1533, 1754, 1777
TatI WGTACW 2 cut(s) 882, 1529
TauI GCSGC 3 cut(s) 246, 297, 2260
TfiI GAWTC 2 cut(s) 2155, 2209
Tru1I TTAA 3 cut(s) 714, 1023, 1043
Tru9I TTAA 3 cut(s) 714, 1023, 1043
TscAI CASTG 9 cut(s) 400, 478, 507, 562, 1257, 1903, 1911, 1989, 2047
TseI GCWGC 4 cut(s) 658, 802, 1085, 1970
TspGWI ACGGA 2 cut(s) 399, 585
TspRI CASTG 9 cut(s) 400, 478, 507, 562, 1257, 1903, 1911, 1989, 2047
VpaK11BI GGWCC 7 cut(s) 27, 248, 336, 450, 1009, 1433, 1959
XagI CCTNNNNNAGG 1 cut(s) 230
XapI RAATTY 2 cut(s) 780, 1777
XmiI GTMKAC 1 cut(s) 1375
XmnI GAANNNNTTC 3 cut(s) 236, 1158, 1934
XspI CTAG 2 cut(s) 705, 1668
ZraI GACGTC 1 cut(s) 720
ZrmI AGTACT 1 cut(s) 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.