RchiOBHm_Chr4g0438031

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009465
Physical Location & Seq
Reverse (-)
60947640 .. 60955355
7716 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40622

Sequence Viewer

Length: 2394 bp
ATGGATCCGACCGGCACCCCTCGCCGGACCCGAAATATCCCGCCGAAACCCGACCTCTACTCCACCGTAGTCGTCCACGGCGACGAAGATTCCACCTCCGAATCCGACCGAGACCGGCGGCGCGGCAACCAGGAACCCGACCCGTACGCCACATTCGTATACAAGAACAATGCTCACGACGAAGACGACGACGACTCCTCACTGCCTCCCCTCCTCAAACGCCTCCCCAAGGACTTCGGCGGCGGCGCCTCCCTCAACTACTTCGACGACGAGGACGACAACGGCGGCGATTTCGGCACCGTGATCGTCAAGAGCGACCGCAATCGGACCACCAGGAGGTCGTCACGTGACTGGGGGAGCCGAGCCGCGGAGGCGGAGGACGGTGACGGCGGAGATGCGTTCTCGACGTTTGTGATGAGGCCGAGTGAGAGAGAGTCGTCGCTGACCGGGACGGTGAGGAGGACCAGCGGCGGCAGTTCCACGATGAGCAGGGCAGTGGCGAGCATGCAGGCCTCCGGCGACGGGATGGGGAAGCAGAGGAAGAGCAGTGCCGCCTCGTCGCAAAACGAGGAGGCTCGCCAGAATATGAAAATGTCGGCCAGTTCCATACCGGAAAGCGTCACCAGAGAAGATCCGACTACAAAGTACGAGTTGCTCAATGAGCTTGGGAAGGGGTCTTATGGTGCGGTTTATAAAGCTAGGGACTTGAAGACTTCCGAGCTGGTGGCAATTAAAGTCATTTCATTATCTCAAGGGGAGGAAGGGTATGAAGAGATTTGTGGTGAAATCGAGATGCTGCAGCAATGCAGTCATCCGAATGTCGTTCGGTACTTGGGGAGCTACCAAGGAGAAGAGTATCTTTGGATTGTGATGGAGTACTGTGGTGGTGGAAGTGTTGCTGATTTAATGAATGTTACCGAGGATGCTTTGGAGGAGTATCAAATAGCTTACATTTGCAGGGAAGCATTGAAGGGCCTTGCTTATTTGCACTCAATTTTCAAGGTCCACAGAGATATTAAGGGTGGTAATATTCTGTTAACTGAACAAGGAGATGTCAAGTTGGGTGACTTTGGGGTTGCAGCACAGCTTACAAGGACCATGTCAAAGCGCAATACGTTCATTGGGACTCCACATTGGATGGCTCCAGAAGTTATTCAGGAAAGCCGCTATGATGGAAAGGTGGATGTATGGGCTCTTGGTGTTTCTGCAATTGAAATGGCTGAGGGACTTCCTCCAAGATCTGCGGTGCATCCAATGAGGGTTCTATTCATGATATCCCTTGAGCCAGCTCCGATGCTTGAGGATAAAGAAAAATGGTCTCTCGTGTTTCATGATTTTGTGGCGAAGTGCCTGACAAAAGAACCTCGGCTACGCCCTACTGCGGCTGAGATGTTGAAGCACAAATTCATCGAAAAATGCAAATGTGGTCCCTCTGCAATGGTGCCAAGGATTGAGAAGGCCAGGCAAATTAGGACCTCTATGGCTTTGCAAGCACAAAATGAAGCTCCAGACGAACAGGAAGACGATACACTTGGAGCTCCCAAAGTGAATGAAGATTATGGAGATACCGTTCCCTCAAGGCCTCTAGGGGCAAATGAAGTGACAGGCAGCACATTGACGAAGCAGCACATGTCTGGTATTGGGGAGCATGCAGAGGAAGGTAATTTTGGCACCGTAATAATTCATGGTGGAGATGAAAGAGATGAGACTGGTACTCGGACACAAATTTTTAATTCCAAAGAAGATTCACCTGCCTCTGGACTTGGCCCTTCCACTGCTGGTACAGTAGGTAAATCAAGCGAAGTCAGGGTGGGAGACGAAAATAACACCTCAGTTGGAGTACGAAATACAGTTACACAGACCAAACCAGCATTTTCTCTTTCAACCTTTACCTCTTCTGAGCCGAACTTGAGAAAATGCAAATCTCAACCACAGGTTGGAAGTCAAAGCAGTGGCACAATGAAGAATGAAACTTTCAGCCGGAAAGCTTTTGCAGTGCAAGATAAGCTTTCGTCCATATATGCAGCTGGTAATACGGTGCCTATTCCATTTTTGAGGGCAACAGATATATCCCCCATTGCTCTCTTATCAGATAATGTTGTTGGAGGCATGCAACGAGATCATAGTGGAACTGGTGCTATAGATGCACTGCAGGAGCTTTTTTCTGGCGATGCACAGTCTAAAAAGGGACGAAGAGGGCAAAATGAGATGCCCCTTCCTCCAAGTGTTTACAAAAGACTCTTTGCAAGTCCAACTCTATTAAATCTCACACAAGCTTTAGCCTACCACAAGATGTGCTATGAAGATATGCCTCTCCAAGAATTGCAAGCAACTCAGGAACAACAGACTATTCAAAATCTTTGCGATACACTAAGAACCATTCTTCGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006950 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007266 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0009893 GO:0009966 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0012501 GO:0016043 GO:0016301 GO:0016310 GO:0016477 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017048 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023051 GO:0023052 GO:0030029 GO:0030036 GO:0031098 GO:0031267 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0040011 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043408 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048365 GO:0048518 GO:0048522 GO:0048583 GO:0048870 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051674 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902531
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

797

Amino Acids

87.36

Weight (kDa)

5.34

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 216 - 470 1.2e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 218 - 466 7.3e-50 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 289 - 367 1.4e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AarI CACCTGC 1 cut(s) 1759
Acc36I ACCTGC 1 cut(s) 1759
AccB1I GGYRCC 6 cut(s) 14, 245, 296, 1441, 1670, 2038
AccB7I CCANNNNNTGG 1 cut(s) 1937
AccI GTMKAC 1 cut(s) 159
AccII CGCG 2 cut(s) 123, 368
AclWI GGATC 2 cut(s) 12, 626
AcoI YGGCCR 1 cut(s) 597
AcsI RAATTY 2 cut(s) 1403, 1725
AcvI CACGTG 1 cut(s) 347
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 7 cut(s) 146, 647, 830, 878, 1714, 1783, 1842
AfiI CCNNNNNNNGG 8 cut(s) 24, 229, 336, 367, 522, 1381, 1757, 1937
AflIII ACRYGT 1 cut(s) 1629
AgsI TTSAA 7 cut(s) 709, 970, 1000, 1214, 1396, 1884, 2354
AjnI CCWGG 3 cut(s) 129, 332, 1460
AjuI GAANNNNNNNTTGG 2 cut(s) 1650, 1682
AloI GAACNNNNNNTCC 2 cut(s) 1554, 1586
Alw21I GWGCWC 1 cut(s) 1540
Alw26I GTCTC 4 cut(s) 105, 1323, 1700, 1809
AlwI GGATC 2 cut(s) 12, 626
AoxI GGCC 7 cut(s) 419, 510, 597, 973, 1458, 1580, 1765
ApeKI GCWGC 6 cut(s) 796, 799, 1079, 1608, 1624, 2024
ApoI RAATTY 2 cut(s) 1403, 1725
Asp700I GAANNNNTTC 1 cut(s) 1152
AspLEI GCGC 3 cut(s) 123, 248, 1110
AspS9I GGNCC 9 cut(s) 27, 327, 462, 973, 1003, 1095, 1427, 1473, 1766
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 6 cut(s) 395, 466, 613, 794, 1076, 1740
AvaII GGWCC 7 cut(s) 27, 327, 462, 1003, 1095, 1427, 1473
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 6 cut(s) 14, 245, 296, 1441, 1670, 2038
BanII GRGCYC 2 cut(s) 1195, 1540
BauI CACGAG 1 cut(s) 1322
BbrPI CACGTG 1 cut(s) 347
BbsI GAAGAC 3 cut(s) 189, 716, 1527
Bbv12I GWGCWC 1 cut(s) 1540
BbvCI CCTCAGC 1 cut(s) 1221
BbvI GCAGC 6 cut(s) 783, 811, 1091, 1620, 1636, 2036
BccI CCATC 4 cut(s) 520, 865, 1132, 1166
BceAI ACGGC 3 cut(s) 94, 298, 403
BcgI CGANNNNNNTGC 2 cut(s) 286, 320
BciT130I CCWGG 3 cut(s) 131, 334, 1462
BcnI CCSGG 1 cut(s) 448
BcoDI GTCTC 4 cut(s) 105, 1323, 1700, 1809
BfaI CTAG 2 cut(s) 699, 1586
BfmI CTRYAG 3 cut(s) 797, 2139, 2150
BfoI RGCGCY 1 cut(s) 249
BfuAI ACCTGC 1 cut(s) 1759
BglI GCCNNNNNGGC 1 cut(s) 371
BglII AGATCT 1 cut(s) 1238
BmcAI AGTACT 1 cut(s) 878
Bme1390I CCNGG 4 cut(s) 131, 334, 448, 1462
Bme18I GGWCC 7 cut(s) 27, 327, 462, 1003, 1095, 1427, 1473
BmgT120I GGNCC 9 cut(s) 27, 327, 462, 973, 1003, 1095, 1427, 1473, 1766
BmrFI CCNGG 4 cut(s) 131, 334, 448, 1462
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 8 cut(s) 385, 783, 913, 1258, 1284, 2134, 2161, 2199
BmuI ACTGGG 1 cut(s) 361
BpiI GAAGAC 3 cut(s) 189, 716, 1527
BpmI CTGGAG 2 cut(s) 1128, 1491
Bpu10I CCTNAGC 1 cut(s) 1221
BpuEI CTTGAG 5 cut(s) 735, 1301, 1319, 1561, 1930
BpuMI CCSGG 1 cut(s) 448
BsaAI YACGTR 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 2 cut(s) 105, 1323
BsaJI CCNNGG 7 cut(s) 76, 228, 366, 844, 918, 1364, 1445
BsaWI WCCGGW 1 cut(s) 610
BsaXI ACNNNNNCTCC 8 cut(s) 44, 74, 179, 209, 749, 779, 1554, 1584
Bsc4I CCNNNNNNNGG 8 cut(s) 24, 229, 336, 367, 522, 1381, 1757, 1937
Bse118I RCCGGY 2 cut(s) 11, 114
Bse1I ACTGG 4 cut(s) 356, 600, 1714, 2137
Bse3DI GCAATG 3 cut(s) 809, 1443, 2076
BseBI CCWGG 3 cut(s) 131, 334, 1462
BseDI CCNNGG 7 cut(s) 76, 228, 366, 844, 918, 1364, 1445
BseGI GGATG 6 cut(s) 531, 811, 928, 1143, 1189, 1249
BseLI CCNNNNNNNGG 8 cut(s) 24, 229, 336, 367, 522, 1381, 1757, 1937
BseMI GCAATG 3 cut(s) 809, 1443, 2076
BseMII CTCAG 5 cut(s) 1212, 1377, 1845, 1890, 2348
BseNI ACTGG 4 cut(s) 356, 600, 1714, 2137
BseRI GAGGAG 5 cut(s) 187, 203, 472, 584, 947
BseXI GCAGC 6 cut(s) 783, 811, 1091, 1620, 1636, 2036
Bsh1236I CGCG 2 cut(s) 123, 368
Bsh1285I CGRYCG 3 cut(s) 12, 109, 319
BshFI GGCC 7 cut(s) 421, 512, 599, 975, 1460, 1582, 1767
BshNI GGYRCC 6 cut(s) 14, 245, 296, 1441, 1670, 2038
BsiEI CGRYCG 3 cut(s) 12, 109, 319
BsiHKAI GWGCWC 1 cut(s) 1540
BsiSI CCGG 7 cut(s) 12, 25, 115, 447, 516, 611, 1981
BsiWI CGTACG 1 cut(s) 144
BslFI GGGAC 6 cut(s) 463, 716, 1138, 1239, 1413, 2202
BslI CCNNNNNNNGG 8 cut(s) 24, 229, 336, 367, 522, 1381, 1757, 1937
BsmAI GTCTC 4 cut(s) 105, 1323, 1700, 1809
BsmBI CGTCTC 1 cut(s) 1809
BsmFI GGGAC 6 cut(s) 463, 716, 1138, 1239, 1413, 2202
BsnI GGCC 7 cut(s) 421, 512, 599, 975, 1460, 1582, 1767
Bso31I GGTCTC 2 cut(s) 105, 1323
Bsp1286I GDGCHC 2 cut(s) 1195, 1540
Bsp143I GATC 5 cut(s) 4, 303, 631, 1238, 2119
BspANI GGCC 7 cut(s) 421, 512, 599, 975, 1460, 1582, 1767
BspCNI CTCAG 5 cut(s) 1213, 1378, 1844, 1891, 2347
BspFNI CGCG 2 cut(s) 123, 368
BspHI TCATGA 2 cut(s) 1269, 1330
BspMAI CTGCAG 2 cut(s) 801, 2154
BspMI ACCTGC 1 cut(s) 1759
BspPI GGATC 2 cut(s) 12, 626
BspQI GCTCTTC 1 cut(s) 536
BspT107I GGYRCC 6 cut(s) 14, 245, 296, 1441, 1670, 2038
BspTNI GGTCTC 2 cut(s) 105, 1323
BsrDI GCAATG 3 cut(s) 809, 1443, 2076
BsrFI RCCGGY 2 cut(s) 11, 114
BsrI ACTGG 4 cut(s) 356, 600, 1714, 2137
BssAI RCCGGY 2 cut(s) 11, 114
BssECI CCNNGG 7 cut(s) 76, 228, 366, 844, 918, 1364, 1445
BssMI GATC 5 cut(s) 4, 303, 631, 1238, 2119
BssNAI GTATAC 1 cut(s) 160
BssNI GRCGYC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 1322
BssT1I CCWWGG 3 cut(s) 228, 844, 1445
Bst1107I GTATAC 1 cut(s) 160
Bst2BI CACGAG 1 cut(s) 1322
Bst2UI CCWGG 3 cut(s) 131, 334, 1462
Bst6I CTCTTC 5 cut(s) 536, 765, 846, 1900, 2188
BstACI GRCGYC 1 cut(s) 246
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 9 cut(s) 502, 506, 510, 577, 1287, 1491, 1650, 2111, 2328
BstDEI CTNAG 6 cut(s) 1221, 1386, 1831, 1899, 2334, 2372
BstDSI CCRYGG 2 cut(s) 76, 366
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 6 cut(s) 531, 811, 928, 1143, 1189, 1249
BstFNI CGCG 2 cut(s) 123, 368
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 3 cut(s) 123, 248, 1110
BstKTI GATC 5 cut(s) 7, 306, 634, 1241, 2122
BstMAI GTCTC 4 cut(s) 105, 1323, 1700, 1809
BstMBI GATC 5 cut(s) 4, 303, 631, 1238, 2119
BstMCI CGRYCG 3 cut(s) 12, 109, 319
BstMWI GCNNNNNNNGC 7 cut(s) 21, 294, 371, 661, 1490, 2005, 2144
BstNI CCWGG 3 cut(s) 131, 334, 1462
BstNSI RCATGY 4 cut(s) 508, 1633, 1652, 2113
BstSCI CCNGG 4 cut(s) 129, 332, 446, 1460
BstSFI CTRYAG 3 cut(s) 797, 2139, 2150
BstUI CGCG 2 cut(s) 123, 368
BstV1I GCAGC 6 cut(s) 783, 811, 1091, 1620, 1636, 2036
BstV2I GAAGAC 3 cut(s) 189, 716, 1527
BstX2I RGATCY 3 cut(s) 4, 631, 1238
BstYI RGATCY 3 cut(s) 4, 631, 1238
BstZ17I GTATAC 1 cut(s) 160
BsuRI GGCC 7 cut(s) 421, 512, 599, 975, 1460, 1582, 1767
BtgI CCRYGG 2 cut(s) 76, 366
BtgZI GCGATG 1 cut(s) 2184
BtsCI GGATG 6 cut(s) 531, 811, 928, 1143, 1189, 1249
BtsI GCAGTG 7 cut(s) 200, 501, 553, 1773, 1957, 2001, 2147
BtsIMutI CAGTG 7 cut(s) 200, 501, 553, 1773, 1957, 2001, 2147
BveI ACCTGC 1 cut(s) 1759
Cac8I GCNNGC 9 cut(s) 502, 506, 510, 577, 1287, 1491, 1650, 2111, 2328
CciI TCATGA 2 cut(s) 1269, 1330
CfoI GCGC 3 cut(s) 123, 248, 1110
Cfr10I RCCGGY 2 cut(s) 11, 114
Cfr13I GGNCC 9 cut(s) 27, 327, 462, 973, 1003, 1095, 1427, 1473, 1766
Cfr42I CCGCGG 1 cut(s) 369
CseI GACGC 1 cut(s) 607
Csp6I GTAC 7 cut(s) 145, 646, 829, 877, 1713, 1782, 1841
CviAII CATG 8 cut(s) 505, 1099, 1270, 1331, 1630, 1649, 1685, 2110
CviQI GTAC 7 cut(s) 145, 646, 829, 877, 1713, 1782, 1841
DdeI CTNAG 6 cut(s) 1221, 1386, 1831, 1899, 2334, 2372
DinI GGCGCC 1 cut(s) 247
DpnI GATC 5 cut(s) 6, 305, 633, 1240, 2121
DpnII GATC 5 cut(s) 4, 303, 631, 1238, 2119
EaeI YGGCCR 1 cut(s) 597
Eam1104I CTCTTC 5 cut(s) 536, 765, 846, 1900, 2188
EarI CTCTTC 5 cut(s) 536, 765, 846, 1900, 2188
EciI GGCGGA 2 cut(s) 389, 405
Ecl136II GAGCTC 1 cut(s) 1538
Eco130I CCWWGG 3 cut(s) 228, 844, 1445
Eco147I AGGCCT 2 cut(s) 512, 1582
Eco24I GRGCYC 2 cut(s) 1195, 1540
Eco31I GGTCTC 2 cut(s) 105, 1323
Eco32I GATATC 1 cut(s) 1275
Eco47I GGWCC 7 cut(s) 27, 327, 462, 1003, 1095, 1427, 1473
Eco53kI GAGCTC 1 cut(s) 1538
Eco72I CACGTG 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 1538
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoO109I RGGNCCY 2 cut(s) 973, 1473
EcoRII CCWGG 3 cut(s) 129, 332, 1460
EcoRV GATATC 1 cut(s) 1275
EcoT14I CCWWGG 3 cut(s) 228, 844, 1445
EcoT38I GRGCYC 2 cut(s) 1195, 1540
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
ErhI CCWWGG 3 cut(s) 228, 844, 1445
Esp3I CGTCTC 1 cut(s) 1809
FaeI CATG 8 cut(s) 508, 1102, 1273, 1334, 1633, 1652, 1688, 2113
FalI AAGNNNNNCTT 4 cut(s) 843, 875, 1992, 2024
FaqI GGGAC 6 cut(s) 463, 716, 1138, 1239, 1413, 2202
FatI CATG 8 cut(s) 504, 1098, 1269, 1330, 1629, 1648, 1684, 2109
FauI CCCGC 1 cut(s) 48
FblI GTMKAC 1 cut(s) 159
FokI GGATG 6 cut(s) 538, 798, 935, 1150, 1196, 1236
FriOI GRGCYC 2 cut(s) 1195, 1540
FspBI CTAG 2 cut(s) 699, 1586
GlaI GCGC 3 cut(s) 122, 247, 1109
GsuI CTGGAG 2 cut(s) 1128, 1491
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 7 cut(s) 421, 512, 599, 975, 1460, 1582, 1767
HapII CCGG 7 cut(s) 12, 25, 115, 447, 516, 611, 1981
HgaI GACGC 1 cut(s) 607
HhaI GCGC 3 cut(s) 123, 248, 1110
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 8 cut(s) 508, 1102, 1273, 1334, 1633, 1652, 1688, 2113
Hin6I GCGC 3 cut(s) 121, 246, 1108
HinP1I GCGC 3 cut(s) 121, 246, 1108
HincII GTYRAC 1 cut(s) 1038
HindII GTYRAC 1 cut(s) 1038
HindIII AAGCTT 3 cut(s) 1986, 2006, 2274
HinfI GANTC 7 cut(s) 89, 101, 194, 434, 1126, 1745, 2238
HpaI GTTAAC 1 cut(s) 1038
HpaII CCGG 7 cut(s) 12, 25, 115, 447, 516, 611, 1981
HphI GGTGA 6 cut(s) 395, 466, 613, 794, 1076, 1740
Hpy166II GTNNAC 5 cut(s) 76, 160, 1006, 1038, 2230
Hpy8I GTNNAC 5 cut(s) 76, 160, 1006, 1038, 2230
HpyAV CCTTC 7 cut(s) 664, 755, 964, 1450, 1652, 1779, 2225
HpyCH4IV ACGT 3 cut(s) 346, 407, 1115
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 294, 371, 661, 1490, 2005, 2144
HpyF3I CTNAG 6 cut(s) 1221, 1386, 1831, 1899, 2334, 2372
HpySE526I ACGT 3 cut(s) 346, 407, 1115
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 8 cut(s) 508, 1102, 1273, 1334, 1633, 1652, 1688, 2113
HspAI GCGC 3 cut(s) 121, 246, 1108
KasI GGCGCC 1 cut(s) 245
KspAI GTTAAC 1 cut(s) 1038
KspI CCGCGG 1 cut(s) 369
Kzo9I GATC 5 cut(s) 4, 303, 631, 1238, 2119
LguI GCTCTTC 1 cut(s) 536
LmnI GCTCC 9 cut(s) 357, 837, 1147, 1294, 1510, 1535, 1543, 1645, 2155
Lsp1109I GCAGC 6 cut(s) 783, 811, 1091, 1620, 1636, 2036
LweI GCATC 8 cut(s) 385, 783, 913, 1258, 1284, 2134, 2161, 2199
MaeI CTAG 2 cut(s) 699, 1586
MaeII ACGT 3 cut(s) 346, 407, 1115
MaeIII GTNAC 8 cut(s) 342, 347, 383, 619, 913, 1064, 1600, 1852
MalI GATC 5 cut(s) 6, 305, 633, 1240, 2121
MboI GATC 5 cut(s) 4, 303, 631, 1238, 2119
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 3 cut(s) 4, 631, 1238
MhlI GDGCHC 2 cut(s) 1195, 1540
Mly113I GGCGCC 1 cut(s) 246
MlyI GAGTC 4 cut(s) 188, 443, 1120, 2232
MmeI TCCRAC 7 cut(s) 32, 129, 659, 1816, 1918, 2083, 2276
MroXI GAANNNNTTC 1 cut(s) 1152
MseI TTAA 6 cut(s) 732, 905, 1017, 1037, 1731, 2261
MslI CAYNNNNRTG 1 cut(s) 816
MspA1I CMGCKG 3 cut(s) 368, 468, 2027
MspI CCGG 7 cut(s) 12, 25, 115, 447, 516, 611, 1981
MspR9I CCNGG 4 cut(s) 131, 334, 448, 1462
MunI CAATTG 1 cut(s) 1209
MvaI CCWGG 3 cut(s) 131, 334, 1462
MvnI CGCG 2 cut(s) 123, 368
MwoI GCNNNNNNNGC 7 cut(s) 21, 294, 371, 661, 1490, 2005, 2144
NarI GGCGCC 1 cut(s) 246
NciI CCSGG 1 cut(s) 448
NdeII GATC 5 cut(s) 4, 303, 631, 1238, 2119
NlaIII CATG 8 cut(s) 508, 1102, 1273, 1334, 1633, 1652, 1688, 2113
NmeAIII GCCGAG 3 cut(s) 386, 447, 1345
NmuCI GTSAC 6 cut(s) 342, 347, 383, 619, 1064, 1600
NspI RCATGY 4 cut(s) 508, 1633, 1652, 2113
PaeI GCATGC 3 cut(s) 508, 1652, 2113
PagI TCATGA 2 cut(s) 1269, 1330
PaqCI CACCTGC 1 cut(s) 1759
PceI AGGCCT 2 cut(s) 512, 1582
PciI ACATGT 1 cut(s) 1629
PciSI GCTCTTC 1 cut(s) 536
PcsI WCGNNNNNNNCGW 7 cut(s) 28, 78, 153, 183, 186, 273, 312
PdmI GAANNNNTTC 1 cut(s) 1152
PfeI GAWTC 3 cut(s) 89, 101, 1745
Pfl23II CGTACG 1 cut(s) 144
PflFI GACNNNGTC 1 cut(s) 1099
PflMI CCANNNNNTGG 1 cut(s) 1937
PleI GAGTC 4 cut(s) 188, 442, 1120, 2232
PluTI GGCGCC 1 cut(s) 249
PmaCI CACGTG 1 cut(s) 347
PmlI CACGTG 1 cut(s) 347
PpsI GAGTC 4 cut(s) 188, 442, 1120, 2232
Ppu21I YACGTR 1 cut(s) 347
PpuMI RGGWCCY 1 cut(s) 1473
PscI ACATGT 1 cut(s) 1629
PsiI TTATAA 1 cut(s) 693
Psp124BI GAGCTC 1 cut(s) 1540
Psp5II RGGWCCY 1 cut(s) 1473
Psp6I CCWGG 3 cut(s) 129, 332, 1460
PspCI CACGTG 1 cut(s) 347
PspGI CCWGG 3 cut(s) 129, 332, 1460
PspLI CGTACG 1 cut(s) 144
PspPI GGNCC 9 cut(s) 27, 327, 462, 973, 1003, 1095, 1427, 1473, 1766
PspPPI RGGWCCY 1 cut(s) 1473
PsrI GAACNNNNNNTAC 2 cut(s) 1353, 1385
PstI CTGCAG 2 cut(s) 801, 2154
PsuI RGATCY 3 cut(s) 4, 631, 1238
PsyI GACNNNGTC 1 cut(s) 1099
PvuII CAGCTG 1 cut(s) 2027
RsaI GTAC 7 cut(s) 146, 647, 830, 878, 1714, 1783, 1842
RsaNI GTAC 7 cut(s) 145, 646, 829, 877, 1713, 1782, 1841
RseI CAYNNNNRTG 1 cut(s) 816
SacI GAGCTC 1 cut(s) 1540
SacII CCGCGG 1 cut(s) 369
SapI GCTCTTC 1 cut(s) 536
SaqAI TTAA 6 cut(s) 732, 905, 1017, 1037, 1731, 2261
Sau3AI GATC 5 cut(s) 4, 303, 631, 1238, 2119
Sau96I GGNCC 9 cut(s) 27, 327, 462, 973, 1003, 1095, 1427, 1473, 1766
ScaI AGTACT 1 cut(s) 878
SchI GAGTC 4 cut(s) 188, 443, 1120, 2232
ScrFI CCNGG 4 cut(s) 131, 334, 448, 1462
SduI GDGCHC 2 cut(s) 1195, 1540
SfaNI GCATC 8 cut(s) 385, 783, 913, 1258, 1284, 2134, 2161, 2199
SfcI CTRYAG 3 cut(s) 797, 2139, 2150
SfoI GGCGCC 1 cut(s) 247
Sfr303I CCGCGG 1 cut(s) 369
SgrBI CCGCGG 1 cut(s) 369
SinI GGWCC 7 cut(s) 27, 327, 462, 1003, 1095, 1427, 1473
SmiMI CAYNNNNRTG 1 cut(s) 816
SmlI CTYRAG 5 cut(s) 750, 1280, 1298, 1576, 1909
SmoI CTYRAG 5 cut(s) 750, 1280, 1298, 1576, 1909
SphI GCATGC 3 cut(s) 508, 1652, 2113
SseBI AGGCCT 2 cut(s) 512, 1582
SspDI GGCGCC 1 cut(s) 245
SspI AATATT 1 cut(s) 1030
SspMI CTAG 2 cut(s) 699, 1586
SstI GAGCTC 1 cut(s) 1540
StuI AGGCCT 2 cut(s) 512, 1582
StyD4I CCNGG 4 cut(s) 129, 332, 446, 1460
StyI CCWWGG 3 cut(s) 228, 844, 1445
TaiI ACGT 3 cut(s) 349, 410, 1118
TaqI TCGA 5 cut(s) 264, 404, 789, 1410, 2386
TaqII GACCGA 1 cut(s) 123
TatI WGTACW 1 cut(s) 876
TfiI GAWTC 3 cut(s) 89, 101, 1745
Tru1I TTAA 6 cut(s) 732, 905, 1017, 1037, 1731, 2261
Tru9I TTAA 6 cut(s) 732, 905, 1017, 1037, 1731, 2261
TscAI CASTG 7 cut(s) 207, 501, 553, 1780, 1957, 2001, 2154
TseFI GTSAC 6 cut(s) 342, 347, 383, 619, 1064, 1600
TseI GCWGC 6 cut(s) 796, 799, 1079, 1608, 1624, 2024
Tsp45I GTSAC 6 cut(s) 342, 347, 383, 619, 1064, 1600
TspRI CASTG 7 cut(s) 207, 501, 553, 1780, 1957, 2001, 2154
Tth111I GACNNNGTC 1 cut(s) 1099
Van91I CCANNNNNTGG 1 cut(s) 1937
VpaK11BI GGWCC 7 cut(s) 27, 327, 462, 1003, 1095, 1427, 1473
XagI CCTNNNNNAGG 1 cut(s) 227
XapI RAATTY 2 cut(s) 1403, 1725
XceI RCATGY 4 cut(s) 508, 1633, 1652, 2113
XmiI GTMKAC 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 1152
XspI CTAG 2 cut(s) 699, 1586
ZrmI AGTACT 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.