pycom13g05380

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009465
Physical Location & Seq
Reverse (-)
3591675 .. 3598422
6748 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g05380.9

Sequence Viewer

Length: 2490 bp
ATGGGTACCCAAGAGCCCAAAAACTACGTCATCGTGCTCAACAGCTGGTTCCCACTGGTTTGCATCCACTCCGAGCTATGGCGTTCTCCAACTGCTAACACACTCTCTCCTTCTATCTCCCTCTCTCTCTCTCTCACTCCCAATCCTGAAATGGATCCGACCCCCACCACCCGCCGGACCCGGAAAATTCCACCCAAACCCGATTACTCAACCGTAGTCATCCATGACGACGATGGCTCCAACTCCGAATCCGATCGTCGGAGCAAACCCAAGAGCTCCGACCCGGAAACCGACCTCTACGCCACAATGGTCTACAAGGGTAATGCCGACGAAGACGAAGACGACGACGCCTCTCTTCCTCCGCTCCTCAAACGCCTCCCCAAGGACTTCGGCGGCGGAGCCCCCATCGATTACTTCGATGACGAGGAGGAAGACGAAAATGGCGGCGATTTTGGCACAATGATCGTCAAGCCCGATCGGAATCGGGCCACGAGGAGGTCGCGCGACTTCAAGCGGGCGTCTTTCGACGATGGCGGAGACGGCGATGGGTTCTCGACCTTTGTGGTGAGGCCGTCGAGTGAGAGAGAGTCTGTCAGCGGGACCGTGGTGAGGAGGACAAGCAGCGGCGTTGGTTCGACGATGAGTAGGGCAGTGGCAAGCATGCAGGCCTCGTCGGAGTCAGGCTTTGGGAAGCAGAGGAGGGGCAGTGGGTCGTCGCAGGGCGAAGAGTATCGCCAAACTACTAAAATGTCGTCGAGTTCAATACCCGATAGCATTACCAGAGAAGACCCAACTGTGAAGTACGACCTGCTCAATGAGCTTGGGAAGGGGTCTTATGGCGCGGTTTATAAAGCTAGGGATATTAAGACGTCGGAGCTGGTGGCCATCAAAGTTATTTCATTGTCTCAAGGGGAGGAGGGGTATGAAGAAATATGCGGTGAAATTGAGATGCTGCAACAATGTAATCATCCGAATGTCGTTCGGTACCTGGGGAGCTACCAAGGAGAAGAGTATCTTTGGATTGTGATGGAGTACTGTGGTGGTGGCAGTGTTGTCGACTTGATGAATGTTACCGAGGATGCTCTGGAGGAGTATCAAATAGCCTTCATTTGTAGGGAGGCGTTGAAGGGCCTTGCTTATCTGCACTCGATTTTCAAGGTCCACAGAGATATTAAGGGTGGTAATATTCTGTTAACTGAACAAGGAGATGTCAAGTTGGGTGATTTTGGTGTTGCAGCTCAACTTACAAGAACCATGTCAAAGCGCAACACGTTCATTGGCACTCCACATTGGATGGCTCCAGAAGTTATTCAGGAAAGTCGTTATGATGGAAAGGTGGATGTATGGGCTCTTGGTGTTTCTGCAATTGAAATGGCAGAGGGCCTCCCTCCAAGATCTTCGGTGCATCCAATGAGGGTTTTATTCATGATATCCCTTGAGCCAGCCCCGATGCTTGAGGATAAAGAAAAATGGTCTCTTGTGTTTCATGATTTTGTGGCAAAGTGCCTAACTAAAGAACCTCGTCTACGCCCTACTGCATCTGAGATGCTGAAGCACAAGTTCATTGAAAAATGCAAATGTGGTCCCTCTGCAATGTTGGCAAAGATTGAAAAGGCAAGGCAAGCAAGAGCTTCAATGGCTTTGCAAGCACAAATTATTGCTCCACCTGAACGGGACAATACAATTGAAGCTCCCAAAGTCAATGAGGATTATGGAGATACTGTCCCTTCAAGGCCACTTCAAGGGGAAAATGAAGTATCTTCAGGTAACTTTGGCACTTTTATTATTCATGAGGGGGATGAAAGAGATGAGATCCCTAGCGAGGTAGAGAGTTTTAGTGCCAAAGAACCTTCACCTGCTCCTGGACTTCCTGAAGGCTCTTCCAATACTGGCGCAGGAGGCAAATTGCCCGAACCCAGGGAGGAAAATTCAAGTGGTATTACCCAAACCAGCATTCCAGTTGTAAACACCCTACGTGCATCTGAGCAGAGCCTAAAGACAAAAGGCATATCTCAAGTCCAGGTTGGAGGTCCAAGCAGTATAAGCAGTGGCACAGTGAAGAATGAAACTGTTAGCCGGAAAGCTTTTGCAATGCAAGATAAGCTTTGGTCCATATATGCAGCTGGTAACACAGTGCCTATACCATTTTTGAGGGCAACCGATATATCCCCCATTGCTCTCTTATCAGACAATGTTCTTGGAGACATGCATCAGGATAAAAGTGGAACTGTAGCCATGGAGGCACTACAGGAGCTTTTTACTGGTGATGGACAGTCTAAGAAGGGACGAAGAGGGCAAAACGAGATGCCCCTCCCTCCAAGTGTTTACAAAAGGCTCTTTACAAGTCCAACTCTAATGAATCTGGCACAAGCATTAGCCTACCACAAGATGTGCTATGAAGATATGGCGCTCCAAGAAATGCAAGCAACTCAGGAACAACAGACTATTCAAAATCTTTGTGATACACTAAGAACCATTCTTAGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006950 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007266 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0009893 GO:0009966 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0012501 GO:0016043 GO:0016301 GO:0016310 GO:0016477 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017048 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023051 GO:0023052 GO:0030029 GO:0030036 GO:0031098 GO:0031267 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0040011 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043408 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048365 GO:0048518 GO:0048522 GO:0048583 GO:0048870 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051674 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902531
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

830

Amino Acids

91.19

Weight (kDa)

5.16

Isoelectric Point (pI)

54.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 849
AarI CACCTGC 1 cut(s) 1864
AatII GACGTC 1 cut(s) 872
Acc36I ACCTGC 2 cut(s) 816, 1864
Acc65I GGTACC 2 cut(s) 5, 984
AccB1I GGYRCC 2 cut(s) 5, 984
AccBSI CCGCTC 1 cut(s) 364
AccI GTMKAC 3 cut(s) 312, 1056, 1525
AccII CGCG 3 cut(s) 502, 504, 842
AclWI GGATC 3 cut(s) 149, 162, 1807
AcoI YGGCCR 1 cut(s) 882
AcsI RAATTY 2 cut(s) 186, 1927
AcuI CTGAAG 3 cut(s) 1571, 1746, 1893
AcyI GRCGYC 3 cut(s) 348, 518, 869
AfaI GTAC 4 cut(s) 7, 803, 986, 1034
AflIII ACRYGT 1 cut(s) 1269
AjnI CCWGG 4 cut(s) 987, 1861, 1916, 2019
AjuI GAANNNNNNNTTGG 2 cut(s) 1938, 1970
Alw21I GWGCWC 2 cut(s) 39, 278
Alw26I GTCTC 4 cut(s) 531, 909, 1479, 2196
AlwI GGATC 3 cut(s) 149, 162, 1807
AoxI GGCC 7 cut(s) 486, 569, 666, 882, 1129, 1381, 1733
ApeKI GCWGC 4 cut(s) 621, 952, 1235, 2120
ApoI RAATTY 2 cut(s) 186, 1927
ArsI GACNNNNNNTTYG 2 cut(s) 12, 44
Asp700I GAANNNNTTC 1 cut(s) 1308
Asp718I GGTACC 2 cut(s) 5, 984
AspLEI GCGC 5 cut(s) 504, 842, 1266, 1895, 2410
AspS9I GGNCC 9 cut(s) 177, 486, 600, 1129, 1159, 1381, 1583, 2030, 2109
AsuC2I CCSGG 2 cut(s) 181, 284
AsuHPI GGTGA 6 cut(s) 577, 619, 950, 1232, 1845, 2276
AvaII GGWCC 6 cut(s) 177, 600, 1159, 1583, 2030, 2109
BalI TGGCCA 1 cut(s) 884
BamHI GGATCC 1 cut(s) 154
BanI GGYRCC 2 cut(s) 5, 984
BanII GRGCYC 4 cut(s) 18, 278, 403, 1351
BauI CACGAG 1 cut(s) 490
BbsI GAAGAC 4 cut(s) 339, 345, 438, 792
Bbv12I GWGCWC 2 cut(s) 39, 278
BbvI GCAGC 4 cut(s) 633, 939, 1247, 2132
BccI CCATC 9 cut(s) 227, 413, 524, 539, 893, 1021, 1288, 1322, 2261
BceAI ACGGC 2 cut(s) 556, 556
BcgI CGANNNNNNTGC 4 cut(s) 445, 479, 1036, 1070
BciT130I CCWGG 4 cut(s) 989, 1863, 1918, 2021
BcnI CCSGG 2 cut(s) 181, 284
BcoDI GTCTC 4 cut(s) 531, 909, 1479, 2196
BfaI CTAG 2 cut(s) 855, 1818
BfmI CTRYAG 2 cut(s) 2229, 2246
BfoI RGCGCY 1 cut(s) 2411
BfuAI ACCTGC 2 cut(s) 816, 1864
BglI GCCNNNNNGGC 1 cut(s) 2240
BglII AGATCT 1 cut(s) 1394
BisI GCNGC 7 cut(s) 394, 445, 622, 625, 953, 1236, 2121
BlsI GCNGC 7 cut(s) 395, 446, 623, 626, 954, 1237, 2122
BmcAI AGTACT 1 cut(s) 1034
Bme1390I CCNGG 6 cut(s) 181, 284, 989, 1863, 1918, 2021
Bme18I GGWCC 6 cut(s) 177, 600, 1159, 1583, 2030, 2109
BmgT120I GGNCC 9 cut(s) 177, 486, 600, 1129, 1159, 1381, 1583, 2030, 2109
BmrFI CCNGG 6 cut(s) 181, 284, 989, 1863, 1918, 2021
BpiI GAAGAC 4 cut(s) 339, 345, 438, 792
BpmI CTGGAG 2 cut(s) 1106, 1284
BpuEI CTTGAG 4 cut(s) 891, 1457, 1475, 1998
BpuMI CCSGG 2 cut(s) 181, 284
Bsa29I ATCGAT 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 1976
BsaBI GATNNNNATC 1 cut(s) 480
BsaHI GRCGYC 3 cut(s) 348, 518, 869
BsaI GGTCTC 1 cut(s) 1479
BsaJI CCNNGG 8 cut(s) 381, 603, 988, 1000, 1074, 1916, 1917, 2235
Bse1I ACTGG 4 cut(s) 60, 1894, 1958, 2266
Bse3DI GCAATG 3 cut(s) 1599, 2097, 2172
Bse8I GATNNNNATC 1 cut(s) 480
BseBI CCWGG 4 cut(s) 989, 1863, 1918, 2021
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 8 cut(s) 381, 603, 988, 1000, 1074, 1916, 1917, 2235
BseGI GGATG 8 cut(s) 63, 219, 967, 1084, 1299, 1345, 1405, 1804
BseJI GATNNNNATC 1 cut(s) 480
BseMI GCAATG 3 cut(s) 1599, 2097, 2172
BseMII CTCAG 3 cut(s) 1533, 1974, 2444
BseNI ACTGG 4 cut(s) 60, 1894, 1958, 2266
BseRI GAGGAG 7 cut(s) 356, 440, 508, 625, 712, 929, 1103
BseXI GCAGC 4 cut(s) 633, 939, 1247, 2132
BsgI GTGCAG 1 cut(s) 1127
Bsh1236I CGCG 3 cut(s) 502, 504, 842
Bsh1285I CGRYCG 2 cut(s) 256, 478
BshFI GGCC 7 cut(s) 488, 571, 668, 884, 1131, 1383, 1735
BshNI GGYRCC 2 cut(s) 5, 984
BshVI ATCGAT 1 cut(s) 408
BsiEI CGRYCG 2 cut(s) 256, 478
BsiHKAI GWGCWC 2 cut(s) 39, 278
BsiSI CCGG 4 cut(s) 175, 181, 284, 2077
BslFI GGGAC 5 cut(s) 613, 1569, 1688, 1709, 2298
BsmAI GTCTC 4 cut(s) 531, 909, 1479, 2196
BsmBI CGTCTC 1 cut(s) 531
BsmFI GGGAC 5 cut(s) 613, 1569, 1688, 1709, 2298
BsmI GAATGC 1 cut(s) 1953
BsnI GGCC 7 cut(s) 488, 571, 668, 884, 1131, 1383, 1735
Bso31I GGTCTC 1 cut(s) 1479
Bsp1286I GDGCHC 5 cut(s) 18, 39, 278, 403, 1351
Bsp143I GATC 6 cut(s) 154, 253, 462, 475, 1394, 1812
Bsp19I CCATGG 1 cut(s) 2235
BspANI GGCC 7 cut(s) 488, 571, 668, 884, 1131, 1383, 1735
BspCNI CTCAG 3 cut(s) 1534, 1975, 2443
BspDI ATCGAT 1 cut(s) 408
BspFNI CGCG 3 cut(s) 502, 504, 842
BspHI TCATGA 3 cut(s) 1425, 1486, 1789
BspMI ACCTGC 2 cut(s) 816, 1864
BspPI GGATC 3 cut(s) 149, 162, 1807
BspQI GCTCTTC 1 cut(s) 1885
BspT107I GGYRCC 2 cut(s) 5, 984
BspTNI GGTCTC 1 cut(s) 1479
BsrBI CCGCTC 1 cut(s) 364
BsrDI GCAATG 3 cut(s) 1599, 2097, 2172
BsrI ACTGG 4 cut(s) 60, 1894, 1958, 2266
BssECI CCNNGG 8 cut(s) 381, 603, 988, 1000, 1074, 1916, 1917, 2235
BssMI GATC 6 cut(s) 154, 253, 462, 475, 1394, 1812
BssNI GRCGYC 3 cut(s) 348, 518, 869
BssSI CACGAG 1 cut(s) 490
BssT1I CCWWGG 3 cut(s) 381, 1000, 2235
Bst2BI CACGAG 1 cut(s) 490
Bst2UI CCWGG 4 cut(s) 989, 1863, 1918, 2021
Bst6I CTCTTC 5 cut(s) 360, 720, 1002, 1885, 2284
BstACI GRCGYC 3 cut(s) 348, 518, 869
BstBAI YACGTR 1 cut(s) 1976
BstC8I GCNNGC 8 cut(s) 516, 658, 662, 666, 1443, 1623, 1647, 2424
BstDEI CTNAG 6 cut(s) 1542, 1983, 2277, 2430, 2468, 2480
BstDSI CCRYGG 2 cut(s) 603, 2235
BstENI CCTNNNNNAGG 1 cut(s) 380
BstF5I GGATG 8 cut(s) 63, 219, 967, 1084, 1299, 1345, 1405, 1804
BstFNI CGCG 3 cut(s) 502, 504, 842
BstH2I RGCGCY 1 cut(s) 2411
BstHHI GCGC 5 cut(s) 504, 842, 1266, 1895, 2410
BstKTI GATC 6 cut(s) 157, 256, 465, 478, 1397, 1815
BstMAI GTCTC 4 cut(s) 531, 909, 1479, 2196
BstMBI GATC 6 cut(s) 154, 253, 462, 475, 1394, 1812
BstMCI CGRYCG 2 cut(s) 256, 478
BstNI CCWGG 4 cut(s) 989, 1863, 1918, 2021
BstNSI RCATGY 2 cut(s) 664, 2209
BstSCI CCNGG 6 cut(s) 179, 282, 987, 1861, 1916, 2019
BstSFI CTRYAG 2 cut(s) 2229, 2246
BstUI CGCG 3 cut(s) 502, 504, 842
BstV1I GCAGC 4 cut(s) 633, 939, 1247, 2132
BstV2I GAAGAC 4 cut(s) 339, 345, 438, 792
BstX2I RGATCY 3 cut(s) 154, 1394, 1812
BstYI RGATCY 3 cut(s) 154, 1394, 1812
Bsu15I ATCGAT 1 cut(s) 408
BsuRI GGCC 7 cut(s) 488, 571, 668, 884, 1131, 1383, 1735
BsuTUI ATCGAT 1 cut(s) 408
BtgI CCRYGG 2 cut(s) 603, 2235
BtgZI GCGATG 1 cut(s) 558
BtsCI GGATG 8 cut(s) 63, 219, 967, 1084, 1299, 1345, 1405, 1804
BtsI GCAGTG 4 cut(s) 657, 712, 1054, 2053
BtsIMutI CAGTG 7 cut(s) 53, 657, 712, 1054, 2053, 2061, 2139
BveI ACCTGC 2 cut(s) 816, 1864
Cac8I GCNNGC 8 cut(s) 516, 658, 662, 666, 1443, 1623, 1647, 2424
CciI TCATGA 3 cut(s) 1425, 1486, 1789
CfoI GCGC 5 cut(s) 504, 842, 1266, 1895, 2410
Cfr13I GGNCC 9 cut(s) 177, 486, 600, 1129, 1159, 1381, 1583, 2030, 2109
ClaI ATCGAT 1 cut(s) 408
CseI GACGC 2 cut(s) 356, 507
Csp6I GTAC 4 cut(s) 6, 802, 985, 1033
CviAII CATG 8 cut(s) 224, 661, 1255, 1426, 1487, 1790, 2206, 2236
CviQI GTAC 4 cut(s) 6, 802, 985, 1033
DdeI CTNAG 6 cut(s) 1542, 1983, 2277, 2430, 2468, 2480
DpnI GATC 6 cut(s) 156, 255, 464, 477, 1396, 1814
DpnII GATC 6 cut(s) 154, 253, 462, 475, 1394, 1812
EaeI YGGCCR 1 cut(s) 882
Eam1104I CTCTTC 5 cut(s) 360, 720, 1002, 1885, 2284
EarI CTCTTC 5 cut(s) 360, 720, 1002, 1885, 2284
EciI GGCGGA 2 cut(s) 411, 549
Ecl136II GAGCTC 1 cut(s) 276
Eco130I CCWWGG 3 cut(s) 381, 1000, 2235
Eco147I AGGCCT 1 cut(s) 668
Eco24I GRGCYC 4 cut(s) 18, 278, 403, 1351
Eco31I GGTCTC 1 cut(s) 1479
Eco32I GATATC 1 cut(s) 1431
Eco47I GGWCC 6 cut(s) 177, 600, 1159, 1583, 2030, 2109
Eco53kI GAGCTC 1 cut(s) 276
Eco57I CTGAAG 3 cut(s) 1571, 1746, 1893
EcoICRI GAGCTC 1 cut(s) 276
EcoNI CCTNNNNNAGG 1 cut(s) 380
EcoO109I RGGNCCY 2 cut(s) 1129, 1381
EcoRII CCWGG 4 cut(s) 987, 1861, 1916, 2019
EcoRV GATATC 1 cut(s) 1431
EcoT14I CCWWGG 3 cut(s) 381, 1000, 2235
EcoT22I ATGCAT 1 cut(s) 2211
EcoT38I GRGCYC 4 cut(s) 18, 278, 403, 1351
ErhI CCWWGG 3 cut(s) 381, 1000, 2235
Esp3I CGTCTC 1 cut(s) 531
FaeI CATG 8 cut(s) 227, 664, 1258, 1429, 1490, 1793, 2209, 2239
FalI AAGNNNNNCTT 4 cut(s) 999, 1031, 2088, 2120
FaqI GGGAC 5 cut(s) 613, 1569, 1688, 1709, 2298
FatI CATG 8 cut(s) 223, 660, 1254, 1425, 1486, 1789, 2205, 2235
FauI CCCGC 3 cut(s) 179, 507, 590
FblI GTMKAC 3 cut(s) 312, 1056, 1525
Fnu4HI GCNGC 7 cut(s) 394, 445, 622, 625, 953, 1236, 2121
FokI GGATG 8 cut(s) 50, 206, 954, 1091, 1306, 1352, 1392, 1811
FriOI GRGCYC 4 cut(s) 18, 278, 403, 1351
Fsp4HI GCNGC 7 cut(s) 394, 445, 622, 625, 953, 1236, 2121
FspBI CTAG 2 cut(s) 855, 1818
GlaI GCGC 5 cut(s) 503, 841, 1265, 1894, 2409
GluI GCNGC 7 cut(s) 394, 445, 622, 625, 953, 1236, 2121
GsuI CTGGAG 2 cut(s) 1106, 1284
HaeII RGCGCY 1 cut(s) 2411
HaeIII GGCC 7 cut(s) 488, 571, 668, 884, 1131, 1383, 1735
HapII CCGG 4 cut(s) 175, 181, 284, 2077
HgaI GACGC 2 cut(s) 356, 507
HhaI GCGC 5 cut(s) 504, 842, 1266, 1895, 2410
Hin1I GRCGYC 3 cut(s) 348, 518, 869
Hin1II CATG 8 cut(s) 227, 664, 1258, 1429, 1490, 1793, 2209, 2239
Hin6I GCGC 5 cut(s) 502, 840, 1264, 1893, 2408
HinP1I GCGC 5 cut(s) 502, 840, 1264, 1893, 2408
HincII GTYRAC 2 cut(s) 1057, 1194
HindII GTYRAC 2 cut(s) 1057, 1194
HindIII AAGCTT 2 cut(s) 2082, 2102
HinfI GANTC 5 cut(s) 248, 481, 587, 677, 2359
HpaI GTTAAC 1 cut(s) 1194
HpaII CCGG 4 cut(s) 175, 181, 284, 2077
HphI GGTGA 6 cut(s) 577, 619, 950, 1232, 1845, 2276
Hpy166II GTNNAC 7 cut(s) 313, 1057, 1162, 1194, 1526, 1966, 2326
Hpy8I GTNNAC 7 cut(s) 313, 1057, 1162, 1194, 1526, 1966, 2326
HpyAV CCTTC 8 cut(s) 120, 820, 1114, 1120, 1737, 1860, 1868, 2275
HpyCH4IV ACGT 4 cut(s) 27, 869, 1271, 1975
HpyF3I CTNAG 6 cut(s) 1542, 1983, 2277, 2430, 2468, 2480
HpySE526I ACGT 4 cut(s) 27, 869, 1271, 1975
Hsp92I GRCGYC 3 cut(s) 348, 518, 869
Hsp92II CATG 8 cut(s) 227, 664, 1258, 1429, 1490, 1793, 2209, 2239
HspAI GCGC 5 cut(s) 502, 840, 1264, 1893, 2408
KpnI GGTACC 2 cut(s) 9, 988
KspAI GTTAAC 1 cut(s) 1194
Kzo9I GATC 6 cut(s) 154, 253, 462, 475, 1394, 1812
LguI GCTCTTC 1 cut(s) 1885
Lsp1109I GCAGC 4 cut(s) 633, 939, 1247, 2132
MaeI CTAG 2 cut(s) 855, 1818
MaeII ACGT 4 cut(s) 27, 869, 1271, 1975
MaeIII GTNAC 3 cut(s) 1069, 1766, 2126
MalI GATC 6 cut(s) 156, 255, 464, 477, 1396, 1814
MbiI CCGCTC 1 cut(s) 364
MboI GATC 6 cut(s) 154, 253, 462, 475, 1394, 1812
MfeI CAATTG 2 cut(s) 1365, 1683
MflI RGATCY 3 cut(s) 154, 1394, 1812
MhlI GDGCHC 5 cut(s) 18, 39, 278, 403, 1351
MlsI TGGCCA 1 cut(s) 884
MluCI AATT 7 cut(s) 186, 942, 1365, 1653, 1683, 1904, 1927
MluNI TGGCCA 1 cut(s) 884
MlyI GAGTC 2 cut(s) 596, 686
MmeI TCCRAC 9 cut(s) 113, 182, 239, 264, 303, 654, 852, 2005, 2372
Mox20I TGGCCA 1 cut(s) 884
Mph1103I ATGCAT 1 cut(s) 2211
MroXI GAANNNNTTC 1 cut(s) 1308
MscI TGGCCA 1 cut(s) 884
MseI TTAA 3 cut(s) 864, 1173, 1193
MslI CAYNNNNRTG 1 cut(s) 972
Msp20I TGGCCA 1 cut(s) 884
MspA1I CMGCKG 4 cut(s) 45, 597, 624, 2123
MspI CCGG 4 cut(s) 175, 181, 284, 2077
MspR9I CCNGG 6 cut(s) 181, 284, 989, 1863, 1918, 2021
MunI CAATTG 2 cut(s) 1365, 1683
Mva1269I GAATGC 1 cut(s) 1953
MvaI CCWGG 4 cut(s) 989, 1863, 1918, 2021
MvnI CGCG 3 cut(s) 502, 504, 842
NciI CCSGG 2 cut(s) 181, 284
NcoI CCATGG 1 cut(s) 2235
NdeII GATC 6 cut(s) 154, 253, 462, 475, 1394, 1812
NlaIII CATG 8 cut(s) 227, 664, 1258, 1429, 1490, 1793, 2209, 2239
NsiI ATGCAT 1 cut(s) 2211
NspI RCATGY 2 cut(s) 664, 2209
PaeI GCATGC 1 cut(s) 664
PagI TCATGA 3 cut(s) 1425, 1486, 1789
PaqCI CACCTGC 1 cut(s) 1864
PasI CCCWGGG 1 cut(s) 1917
PceI AGGCCT 1 cut(s) 668
PciSI GCTCTTC 1 cut(s) 1885
PcsI WCGNNNNNNNCGW 3 cut(s) 342, 414, 471
PctI GAATGC 1 cut(s) 1953
PdmI GAANNNNTTC 1 cut(s) 1308
PfeI GAWTC 3 cut(s) 248, 481, 2359
PfoI TCCNGGA 1 cut(s) 1861
PkrI GCNGC 7 cut(s) 395, 446, 623, 626, 954, 1237, 2122
Ple19I CGATCG 2 cut(s) 256, 478
PleI GAGTC 2 cut(s) 595, 685
PpsI GAGTC 2 cut(s) 595, 685
Ppu21I YACGTR 1 cut(s) 1976
PsiI TTATAA 1 cut(s) 849
Psp124BI GAGCTC 1 cut(s) 278
Psp6I CCWGG 4 cut(s) 987, 1861, 1916, 2019
PspGI CCWGG 4 cut(s) 987, 1861, 1916, 2019
PspPI GGNCC 9 cut(s) 177, 486, 600, 1129, 1159, 1381, 1583, 2030, 2109
PsrI GAACNNNNNNTAC 2 cut(s) 1509, 1541
PsuI RGATCY 3 cut(s) 154, 1394, 1812
PvuI CGATCG 2 cut(s) 256, 478
PvuII CAGCTG 2 cut(s) 45, 2123
RsaI GTAC 4 cut(s) 7, 803, 986, 1034
RsaNI GTAC 4 cut(s) 6, 802, 985, 1033
RseI CAYNNNNRTG 1 cut(s) 972
SacI GAGCTC 1 cut(s) 278
SalI GTCGAC 1 cut(s) 1055
SapI GCTCTTC 1 cut(s) 1885
SaqAI TTAA 3 cut(s) 864, 1173, 1193
SatI GCNGC 7 cut(s) 394, 445, 622, 625, 953, 1236, 2121
Sau3AI GATC 6 cut(s) 154, 253, 462, 475, 1394, 1812
Sau96I GGNCC 9 cut(s) 177, 486, 600, 1129, 1159, 1381, 1583, 2030, 2109
ScaI AGTACT 1 cut(s) 1034
SchI GAGTC 2 cut(s) 596, 686
ScrFI CCNGG 6 cut(s) 181, 284, 989, 1863, 1918, 2021
SduI GDGCHC 5 cut(s) 18, 39, 278, 403, 1351
SfcI CTRYAG 2 cut(s) 2229, 2246
SinI GGWCC 6 cut(s) 177, 600, 1159, 1583, 2030, 2109
SmiMI CAYNNNNRTG 1 cut(s) 972
SmlI CTYRAG 4 cut(s) 906, 1436, 1454, 2013
SmoI CTYRAG 4 cut(s) 906, 1436, 1454, 2013
SphI GCATGC 1 cut(s) 664
Sse9I AATT 7 cut(s) 186, 942, 1365, 1653, 1683, 1904, 1927
SseBI AGGCCT 1 cut(s) 668
SspI AATATT 1 cut(s) 1186
SspMI CTAG 2 cut(s) 855, 1818
SstI GAGCTC 1 cut(s) 278
StuI AGGCCT 1 cut(s) 668
StyD4I CCNGG 6 cut(s) 179, 282, 987, 1861, 1916, 2019
StyI CCWWGG 3 cut(s) 381, 1000, 2235
TaiI ACGT 4 cut(s) 30, 872, 1274, 1978
TaqI TCGA 9 cut(s) 408, 417, 525, 554, 575, 635, 755, 1056, 1148
TasI AATT 7 cut(s) 186, 942, 1365, 1653, 1683, 1904, 1927
TatI WGTACW 1 cut(s) 1032
TauI GCSGC 3 cut(s) 396, 447, 627
TfiI GAWTC 3 cut(s) 248, 481, 2359
Tru1I TTAA 3 cut(s) 864, 1173, 1193
Tru9I TTAA 3 cut(s) 864, 1173, 1193
TscAI CASTG 7 cut(s) 60, 657, 712, 1054, 2053, 2061, 2139
TseI GCWGC 4 cut(s) 621, 952, 1235, 2120
TspRI CASTG 7 cut(s) 60, 657, 712, 1054, 2053, 2061, 2139
VpaK11BI GGWCC 6 cut(s) 177, 600, 1159, 1583, 2030, 2109
XagI CCTNNNNNAGG 1 cut(s) 380
XapI RAATTY 2 cut(s) 186, 1927
XceI RCATGY 2 cut(s) 664, 2209
XcmI CCANNNNNNNNNTGG 2 cut(s) 148, 230
XmiI GTMKAC 3 cut(s) 312, 1056, 1525
XmnI GAANNNNTTC 1 cut(s) 1308
XspI CTAG 2 cut(s) 855, 1818
ZraI GACGTC 1 cut(s) 870
ZrmI AGTACT 1 cut(s) 1034
Zsp2I ATGCAT 1 cut(s) 2211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.