Rw0G016190

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00751
OG0009465
Physical Location & Seq
Reverse (-)
135658 .. 144729
9072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G016190.1

Sequence Viewer

Length: 2388 bp
ATGAATCCGACCGGCACCCCTCGCCGGACCCGAAATATCCCGCCGAAACCCGACCTCTACTCCACCGTAGTCGTCCACGGCGACGAAGATTCCACCTCCGAATCCGACCGAGACCGGCGGCGCGGCAACCAGGAACCCGACCCGTACGCCACATTCGTATACAAGAACAACGCTCACGACGAAGACGACGACGACTCCTCACTCCCTCCCCTCCTCAAACGCCTCCCCAAGGACTTCGGCGGCGGCGCCTCCCTCAACTACTTCGACGACGAGGACGACAACGGCGGCGATTTCGGCACCGTGATCGTCAAGAGCGACCGCAATCGGACCACCAGGAGGTCGTCACGTGACTGGGGGAGCCGAGCCGCGGAGGCGGAGGACGGTGACGGCGGAGCTGCGTTCTCGACGTTTGTGATGAGGCCGAGTGAGAGAGAGTCGTCGCTGACCGGGACGGTGAGGAGGACCAGCGGCGGCAGTTCCACGATGAGCAGGGCGGTGGCGAGTATGCAGGCCTCCGGCGACGGGATGGGGAAGCAGAGGAAGAGCAGTGCCGCCTCGTCGCAAAACGAGGAGGCTCGCCAGAATATGAAAATGTCGACCAGTTCCATACCGGAAAGCGTCACCAGAGAAGATCCGACTACAAAGTACGAGTTGCTCAATGAGCTTGGGAAGGGGTCTTATGGTGCGGTTTATAAAGCTAGGGACTTAAAGACTTCCGAGCTGGTGGCAATTAAAGTCATTTCATTATCTCAAGGGGAGGAAGGGTATGAAGAGATTTGTGGTGAAATCGAGATGCTGCAGCAATGCAGTCATCCCAATGTCGTTCGGTACTTGGGGAGCTACCAAGGAGAAGAGTATCTTTGGATTGTGATGGAGTACTGTGGTGGTGGAAGTGTTGCTGATTTAATGAATGTTACCGAGGATGCTTTGGAGGAGTATCAAATAGCTTACATTTGCAGGGAAGCATTGAAGGGCCTTGCTTATTTGCACTCAATTTTCAAGGTCCACAGAGATATTAAGGGTGGTAATATTCTGCTAACTGAACAAGGAGATGTCAAGTTGGGTGACTTTGGGGTTGCAGCACAGCTTACAAGGACCATGTCAAAGCGCAATACGTTCATTGGGACTCCACATTGGATGGCTCCAGAAGTTATTCAGGAAAGCCGCTATGATGGAAAGGTGGATGTATGGGCTCTTGGTGTTTCTGCAATTGAAATGGCTGAGGGACTTCCTCCAAGATCTGCGGTGCATCCAATGAGGGTTCTATTCATGATATCCCTTGAGCCAGCTCCGATGCTTGAGGATAAAGAAAAATGGTCTCTCGTGTTTCATGATTTTGTGGCGAAGTGCCTGACAAAAGAACCTCGGCTACGCCCTACTGCAGCTGAGATGTTGAAGCACAAATTCATCGAAAAATGCAAATGTGGTCCCTCTGCAATGGTGCCAAGGATTGAGAAGGCCAGGCAAATTAGGACCTCTATGGCTTTGCAAGCACAAAATGAAGCTCCAGATGAACAGGAAGACGATACACTTGAAGCTCCCAAAGTGAATGAAGATTATGGAGATACCGTTCCCTCAAGGCCTGTAGGGGCAAATGAAGTGACAGGCAGCACATTGACGAAGCAGCACATGTCTGGTATTGGGGAGCATGCAGAGGAAGGTAATTTTGGCACAGTAATAATTCATGGTGGAGATGAAAGAGATGAGACTGGTACTCGGACACAAATTTTTAATTCCAAAGAAGATTCACCTGGACTTGGCCCTTCCACTGCTGGTACAGTAGGTAAATCAAGCGAAGTCAGGGTGGGAGACGAAAATAGCACCTCAGTTGGAGTACGAAATACAGTTACACAGGCCAAACCAGCATTTTCTCTTTCAACCTTTACCTCTTCTGAGCCGAACTTGAGAAAATGCAAATCTCAACCACAGGTTGGAAGTCAAAGCAGTGGCACAGTGAAGAATGAAACTTTCAGCCGGAAAGCTTTTGCAGTGCAAGATAAGCTTTGGTCCATATATGCAGCTGGTAATACAGTGCCTATTCCATTTTTGAGGGCAACAGATATATCCCCCATTGCTCTCTTATCTGATAATGTTGTTGGAGGCATGCAACGAGATCATAGTGGAACTGGTGCTATAGATGCACTACAGGAGCTTTTTTCTGGCGATGCACAGTCTAAAAAGGGACGAAGAGGGCAAAATGAGATGCCCCTTCCTCCAAGTGTTTACAAAAGACTCTTTGCAAGTCCAACTCTATTAAATCTCACACAAGCTTTAGCCTACCACAAGATGTGCTATGAAGATATGCCTCTCCAAGAATTGCAAGCAACTCAGGAACAACAGACTATTCAAAATCTTTGCGATACACTAAGAACCATTCTTCGATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006950 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007266 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0009893 GO:0009966 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0012501 GO:0016043 GO:0016301 GO:0016310 GO:0016477 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017048 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023051 GO:0023052 GO:0030029 GO:0030036 GO:0031098 GO:0031267 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0040011 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043408 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048365 GO:0048518 GO:0048522 GO:0048583 GO:0048870 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051674 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902531
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

795

Amino Acids

87.29

Weight (kDa)

5.38

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 216 - 470 1.2e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 218 - 466 7.3e-50 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 289 - 367 1.4e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AccB1I GGYRCC 4 cut(s) 14, 245, 296, 1441
AccB7I CCANNNNNTGG 1 cut(s) 1931
AccI GTMKAC 2 cut(s) 159, 596
AccII CGCG 2 cut(s) 123, 368
AclWI GGATC 1 cut(s) 626
AcsI RAATTY 2 cut(s) 1403, 1725
AcvI CACGTG 1 cut(s) 347
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 7 cut(s) 146, 647, 830, 878, 1714, 1777, 1836
AfiI CCNNNNNNNGG 7 cut(s) 24, 229, 336, 367, 522, 1757, 1931
AflIII ACRYGT 1 cut(s) 1629
AgsI TTSAA 7 cut(s) 970, 1000, 1214, 1396, 1535, 1878, 2348
AjnI CCWGG 4 cut(s) 129, 332, 1460, 1750
AjuI GAANNNNNNNTTGG 2 cut(s) 1650, 1682
AloI GAACNNNNNNTCC 2 cut(s) 1554, 1586
Alw26I GTCTC 4 cut(s) 105, 1323, 1700, 1803
AlwI GGATC 1 cut(s) 626
AoxI GGCC 7 cut(s) 419, 510, 973, 1458, 1580, 1759, 1854
ApeKI GCWGC 8 cut(s) 395, 796, 799, 1079, 1382, 1608, 1624, 2018
ApoI RAATTY 2 cut(s) 1403, 1725
Asp700I GAANNNNTTC 1 cut(s) 1152
AspLEI GCGC 3 cut(s) 123, 248, 1110
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 6 cut(s) 395, 466, 613, 794, 1076, 1740
AvaII GGWCC 8 cut(s) 27, 327, 462, 1003, 1095, 1427, 1473, 2007
BanI GGYRCC 4 cut(s) 14, 245, 296, 1441
BanII GRGCYC 1 cut(s) 1195
BauI CACGAG 1 cut(s) 1322
BbrPI CACGTG 1 cut(s) 347
BbsI GAAGAC 2 cut(s) 189, 1527
BbvCI CCTCAGC 1 cut(s) 1221
BbvI GCAGC 8 cut(s) 382, 783, 811, 1091, 1394, 1620, 1636, 2030
BccI CCATC 4 cut(s) 520, 865, 1132, 1166
BceAI ACGGC 3 cut(s) 94, 298, 403
BcgI CGANNNNNNTGC 2 cut(s) 286, 320
BciT130I CCWGG 4 cut(s) 131, 334, 1462, 1752
BcnI CCSGG 1 cut(s) 448
BcoDI GTCTC 4 cut(s) 105, 1323, 1700, 1803
BfaI CTAG 1 cut(s) 699
BfmI CTRYAG 5 cut(s) 797, 1380, 1584, 2133, 2144
BfoI RGCGCY 1 cut(s) 249
BglI GCCNNNNNGGC 1 cut(s) 371
BglII AGATCT 1 cut(s) 1238
BmcAI AGTACT 1 cut(s) 878
Bme1390I CCNGG 5 cut(s) 131, 334, 448, 1462, 1752
Bme18I GGWCC 8 cut(s) 27, 327, 462, 1003, 1095, 1427, 1473, 2007
BmiI GGNNCC 9 cut(s) 16, 29, 135, 247, 298, 359, 1143, 1429, 1443
BmrFI CCNGG 5 cut(s) 131, 334, 448, 1462, 1752
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 7 cut(s) 783, 913, 1258, 1284, 2128, 2155, 2193
BmuI ACTGGG 1 cut(s) 361
BpiI GAAGAC 2 cut(s) 189, 1527
BpmI CTGGAG 2 cut(s) 1128, 1491
Bpu10I CCTNAGC 1 cut(s) 1221
BpuEI CTTGAG 5 cut(s) 735, 1301, 1319, 1561, 1924
BpuMI CCSGG 1 cut(s) 448
BsaAI YACGTR 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 2 cut(s) 105, 1323
BsaJI CCNNGG 7 cut(s) 76, 228, 366, 844, 918, 1364, 1445
BsaWI WCCGGW 1 cut(s) 610
BsaXI ACNNNNNCTCC 8 cut(s) 44, 74, 179, 209, 749, 779, 1554, 1584
Bsc4I CCNNNNNNNGG 7 cut(s) 24, 229, 336, 367, 522, 1757, 1931
Bse118I RCCGGY 2 cut(s) 11, 114
Bse1I ACTGG 4 cut(s) 356, 600, 1714, 2131
Bse3DI GCAATG 3 cut(s) 809, 1443, 2070
BseBI CCWGG 4 cut(s) 131, 334, 1462, 1752
BseDI CCNNGG 7 cut(s) 76, 228, 366, 844, 918, 1364, 1445
BseGI GGATG 6 cut(s) 531, 811, 928, 1143, 1189, 1249
BseLI CCNNNNNNNGG 7 cut(s) 24, 229, 336, 367, 522, 1757, 1931
BseMI GCAATG 3 cut(s) 809, 1443, 2070
BseMII CTCAG 5 cut(s) 1212, 1377, 1839, 1884, 2342
BseNI ACTGG 4 cut(s) 356, 600, 1714, 2131
BseRI GAGGAG 5 cut(s) 187, 203, 472, 584, 947
BseXI GCAGC 8 cut(s) 382, 783, 811, 1091, 1394, 1620, 1636, 2030
Bsh1236I CGCG 2 cut(s) 123, 368
Bsh1285I CGRYCG 3 cut(s) 12, 109, 319
BshFI GGCC 7 cut(s) 421, 512, 975, 1460, 1582, 1761, 1856
BshNI GGYRCC 4 cut(s) 14, 245, 296, 1441
BsiEI CGRYCG 3 cut(s) 12, 109, 319
BsiSI CCGG 7 cut(s) 12, 25, 115, 447, 516, 611, 1975
BsiWI CGTACG 1 cut(s) 144
BslFI GGGAC 6 cut(s) 463, 716, 1138, 1239, 1413, 2196
BslI CCNNNNNNNGG 7 cut(s) 24, 229, 336, 367, 522, 1757, 1931
BsmAI GTCTC 4 cut(s) 105, 1323, 1700, 1803
BsmBI CGTCTC 1 cut(s) 1803
BsmFI GGGAC 6 cut(s) 463, 716, 1138, 1239, 1413, 2196
BsnI GGCC 7 cut(s) 421, 512, 975, 1460, 1582, 1761, 1856
Bso31I GGTCTC 2 cut(s) 105, 1323
Bsp1286I GDGCHC 1 cut(s) 1195
Bsp143I GATC 4 cut(s) 303, 631, 1238, 2113
BspANI GGCC 7 cut(s) 421, 512, 975, 1460, 1582, 1761, 1856
BspCNI CTCAG 5 cut(s) 1213, 1378, 1838, 1885, 2341
BspFNI CGCG 2 cut(s) 123, 368
BspHI TCATGA 2 cut(s) 1269, 1330
BspLI GGNNCC 9 cut(s) 16, 29, 135, 247, 298, 359, 1143, 1429, 1443
BspMAI CTGCAG 2 cut(s) 801, 1384
BspPI GGATC 1 cut(s) 626
BspQI GCTCTTC 1 cut(s) 536
BspT107I GGYRCC 4 cut(s) 14, 245, 296, 1441
BspTNI GGTCTC 2 cut(s) 105, 1323
BsrDI GCAATG 3 cut(s) 809, 1443, 2070
BsrFI RCCGGY 2 cut(s) 11, 114
BsrI ACTGG 4 cut(s) 356, 600, 1714, 2131
BssAI RCCGGY 2 cut(s) 11, 114
BssECI CCNNGG 7 cut(s) 76, 228, 366, 844, 918, 1364, 1445
BssMI GATC 4 cut(s) 303, 631, 1238, 2113
BssNAI GTATAC 1 cut(s) 160
BssNI GRCGYC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 1322
BssT1I CCWWGG 3 cut(s) 228, 844, 1445
Bst1107I GTATAC 1 cut(s) 160
Bst2BI CACGAG 1 cut(s) 1322
Bst2UI CCWGG 4 cut(s) 131, 334, 1462, 1752
Bst6I CTCTTC 5 cut(s) 536, 765, 846, 1894, 2182
BstACI GRCGYC 1 cut(s) 246
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 7 cut(s) 510, 577, 1287, 1491, 1650, 2105, 2322
BstDEI CTNAG 6 cut(s) 1221, 1386, 1825, 1893, 2328, 2366
BstDSI CCRYGG 2 cut(s) 76, 366
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 6 cut(s) 531, 811, 928, 1143, 1189, 1249
BstFNI CGCG 2 cut(s) 123, 368
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 3 cut(s) 123, 248, 1110
BstKTI GATC 4 cut(s) 306, 634, 1241, 2116
BstMAI GTCTC 4 cut(s) 105, 1323, 1700, 1803
BstMBI GATC 4 cut(s) 303, 631, 1238, 2113
BstMCI CGRYCG 3 cut(s) 12, 109, 319
BstMWI GCNNNNNNNGC 8 cut(s) 21, 294, 371, 661, 1490, 1862, 1999, 2138
BstNI CCWGG 4 cut(s) 131, 334, 1462, 1752
BstNSI RCATGY 3 cut(s) 1633, 1652, 2107
BstSCI CCNGG 5 cut(s) 129, 332, 446, 1460, 1750
BstSFI CTRYAG 5 cut(s) 797, 1380, 1584, 2133, 2144
BstUI CGCG 2 cut(s) 123, 368
BstV1I GCAGC 8 cut(s) 382, 783, 811, 1091, 1394, 1620, 1636, 2030
BstV2I GAAGAC 2 cut(s) 189, 1527
BstX2I RGATCY 2 cut(s) 631, 1238
BstYI RGATCY 2 cut(s) 631, 1238
BstZ17I GTATAC 1 cut(s) 160
BsuRI GGCC 7 cut(s) 421, 512, 975, 1460, 1582, 1761, 1856
BtgI CCRYGG 2 cut(s) 76, 366
BtgZI GCGATG 1 cut(s) 2178
BtsCI GGATG 6 cut(s) 531, 811, 928, 1143, 1189, 1249
BtsI GCAGTG 4 cut(s) 553, 1767, 1951, 1995
BtsIMutI CAGTG 6 cut(s) 553, 1767, 1951, 1959, 1995, 2037
Cac8I GCNNGC 7 cut(s) 510, 577, 1287, 1491, 1650, 2105, 2322
CciI TCATGA 2 cut(s) 1269, 1330
CfoI GCGC 3 cut(s) 123, 248, 1110
Cfr10I RCCGGY 2 cut(s) 11, 114
Cfr42I CCGCGG 1 cut(s) 369
CseI GACGC 1 cut(s) 607
Csp6I GTAC 7 cut(s) 145, 646, 829, 877, 1713, 1776, 1835
CviAII CATG 7 cut(s) 1099, 1270, 1331, 1630, 1649, 1685, 2104
CviQI GTAC 7 cut(s) 145, 646, 829, 877, 1713, 1776, 1835
DdeI CTNAG 6 cut(s) 1221, 1386, 1825, 1893, 2328, 2366
DinI GGCGCC 1 cut(s) 247
DpnI GATC 4 cut(s) 305, 633, 1240, 2115
DpnII GATC 4 cut(s) 303, 631, 1238, 2113
Eam1104I CTCTTC 5 cut(s) 536, 765, 846, 1894, 2182
EarI CTCTTC 5 cut(s) 536, 765, 846, 1894, 2182
EciI GGCGGA 2 cut(s) 389, 405
Eco130I CCWWGG 3 cut(s) 228, 844, 1445
Eco147I AGGCCT 2 cut(s) 512, 1582
Eco24I GRGCYC 1 cut(s) 1195
Eco31I GGTCTC 2 cut(s) 105, 1323
Eco32I GATATC 1 cut(s) 1275
Eco47I GGWCC 8 cut(s) 27, 327, 462, 1003, 1095, 1427, 1473, 2007
Eco72I CACGTG 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoO109I RGGNCCY 2 cut(s) 973, 1473
EcoRII CCWGG 4 cut(s) 129, 332, 1460, 1750
EcoRV GATATC 1 cut(s) 1275
EcoT14I CCWWGG 3 cut(s) 228, 844, 1445
EcoT38I GRGCYC 1 cut(s) 1195
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
ErhI CCWWGG 3 cut(s) 228, 844, 1445
Esp3I CGTCTC 1 cut(s) 1803
FaeI CATG 7 cut(s) 1102, 1273, 1334, 1633, 1652, 1688, 2107
FalI AAGNNNNNCTT 4 cut(s) 843, 875, 1986, 2018
FaqI GGGAC 6 cut(s) 463, 716, 1138, 1239, 1413, 2196
FatI CATG 7 cut(s) 1098, 1269, 1330, 1629, 1648, 1684, 2103
FauI CCCGC 1 cut(s) 48
FblI GTMKAC 2 cut(s) 159, 596
FokI GGATG 6 cut(s) 538, 798, 935, 1150, 1196, 1236
FriOI GRGCYC 1 cut(s) 1195
FspBI CTAG 1 cut(s) 699
GlaI GCGC 3 cut(s) 122, 247, 1109
GsuI CTGGAG 2 cut(s) 1128, 1491
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 7 cut(s) 421, 512, 975, 1460, 1582, 1761, 1856
HapII CCGG 7 cut(s) 12, 25, 115, 447, 516, 611, 1975
HgaI GACGC 1 cut(s) 607
HhaI GCGC 3 cut(s) 123, 248, 1110
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 7 cut(s) 1102, 1273, 1334, 1633, 1652, 1688, 2107
Hin6I GCGC 3 cut(s) 121, 246, 1108
HinP1I GCGC 3 cut(s) 121, 246, 1108
HincII GTYRAC 1 cut(s) 597
HindII GTYRAC 1 cut(s) 597
HindIII AAGCTT 3 cut(s) 1980, 2000, 2268
HinfI GANTC 9 cut(s) 4, 89, 101, 194, 434, 1126, 1745, 2232, 2382
HpaII CCGG 7 cut(s) 12, 25, 115, 447, 516, 611, 1975
HphI GGTGA 6 cut(s) 395, 466, 613, 794, 1076, 1740
Hpy166II GTNNAC 5 cut(s) 76, 160, 597, 1006, 2224
Hpy8I GTNNAC 5 cut(s) 76, 160, 597, 1006, 2224
HpyAV CCTTC 7 cut(s) 664, 755, 964, 1450, 1652, 1773, 2219
HpyCH4IV ACGT 3 cut(s) 346, 407, 1115
HpyF10VI GCNNNNNNNGC 8 cut(s) 21, 294, 371, 661, 1490, 1862, 1999, 2138
HpyF3I CTNAG 6 cut(s) 1221, 1386, 1825, 1893, 2328, 2366
HpySE526I ACGT 3 cut(s) 346, 407, 1115
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 7 cut(s) 1102, 1273, 1334, 1633, 1652, 1688, 2107
HspAI GCGC 3 cut(s) 121, 246, 1108
KasI GGCGCC 1 cut(s) 245
KspI CCGCGG 1 cut(s) 369
Kzo9I GATC 4 cut(s) 303, 631, 1238, 2113
LguI GCTCTTC 1 cut(s) 536
LmnI GCTCC 9 cut(s) 357, 392, 837, 1147, 1294, 1510, 1543, 1645, 2149
Lsp1109I GCAGC 8 cut(s) 382, 783, 811, 1091, 1394, 1620, 1636, 2030
LweI GCATC 7 cut(s) 783, 913, 1258, 1284, 2128, 2155, 2193
MaeI CTAG 1 cut(s) 699
MaeII ACGT 3 cut(s) 346, 407, 1115
MaeIII GTNAC 8 cut(s) 342, 347, 383, 619, 913, 1064, 1600, 1846
MalI GATC 4 cut(s) 305, 633, 1240, 2115
MboI GATC 4 cut(s) 303, 631, 1238, 2113
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 2 cut(s) 631, 1238
MhlI GDGCHC 1 cut(s) 1195
Mly113I GGCGCC 1 cut(s) 246
MlyI GAGTC 4 cut(s) 188, 443, 1120, 2226
MmeI TCCRAC 7 cut(s) 32, 129, 659, 1810, 1912, 2077, 2270
MroXI GAANNNNTTC 1 cut(s) 1152
MseI TTAA 6 cut(s) 707, 732, 905, 1017, 1731, 2255
MslI CAYNNNNRTG 1 cut(s) 816
MspA1I CMGCKG 4 cut(s) 368, 468, 1385, 2021
MspI CCGG 7 cut(s) 12, 25, 115, 447, 516, 611, 1975
MspR9I CCNGG 5 cut(s) 131, 334, 448, 1462, 1752
MunI CAATTG 1 cut(s) 1209
MvaI CCWGG 4 cut(s) 131, 334, 1462, 1752
MvnI CGCG 2 cut(s) 123, 368
MwoI GCNNNNNNNGC 8 cut(s) 21, 294, 371, 661, 1490, 1862, 1999, 2138
NarI GGCGCC 1 cut(s) 246
NciI CCSGG 1 cut(s) 448
NdeII GATC 4 cut(s) 303, 631, 1238, 2113
NlaIII CATG 7 cut(s) 1102, 1273, 1334, 1633, 1652, 1688, 2107
NlaIV GGNNCC 9 cut(s) 16, 29, 135, 247, 298, 359, 1143, 1429, 1443
NmeAIII GCCGAG 3 cut(s) 386, 447, 1345
NmuCI GTSAC 6 cut(s) 342, 347, 383, 619, 1064, 1600
NspI RCATGY 3 cut(s) 1633, 1652, 2107
PaeI GCATGC 2 cut(s) 1652, 2107
PagI TCATGA 2 cut(s) 1269, 1330
PceI AGGCCT 2 cut(s) 512, 1582
PciI ACATGT 1 cut(s) 1629
PciSI GCTCTTC 1 cut(s) 536
PcsI WCGNNNNNNNCGW 8 cut(s) 28, 78, 153, 177, 183, 186, 273, 312
PdmI GAANNNNTTC 1 cut(s) 1152
PfeI GAWTC 5 cut(s) 4, 89, 101, 1745, 2382
Pfl23II CGTACG 1 cut(s) 144
PflFI GACNNNGTC 1 cut(s) 1099
PflMI CCANNNNNTGG 1 cut(s) 1931
PleI GAGTC 4 cut(s) 188, 442, 1120, 2226
PluTI GGCGCC 1 cut(s) 249
PmaCI CACGTG 1 cut(s) 347
PmlI CACGTG 1 cut(s) 347
PpsI GAGTC 4 cut(s) 188, 442, 1120, 2226
Ppu21I YACGTR 1 cut(s) 347
PpuMI RGGWCCY 1 cut(s) 1473
PscI ACATGT 1 cut(s) 1629
PsiI TTATAA 1 cut(s) 693
Psp5II RGGWCCY 1 cut(s) 1473
Psp6I CCWGG 4 cut(s) 129, 332, 1460, 1750
PspCI CACGTG 1 cut(s) 347
PspGI CCWGG 4 cut(s) 129, 332, 1460, 1750
PspLI CGTACG 1 cut(s) 144
PspN4I GGNNCC 9 cut(s) 16, 29, 135, 247, 298, 359, 1143, 1429, 1443
PspPPI RGGWCCY 1 cut(s) 1473
PsrI GAACNNNNNNTAC 2 cut(s) 1353, 1385
PstI CTGCAG 2 cut(s) 801, 1384
PsuI RGATCY 2 cut(s) 631, 1238
PsyI GACNNNGTC 1 cut(s) 1099
PvuII CAGCTG 2 cut(s) 1385, 2021
RsaI GTAC 7 cut(s) 146, 647, 830, 878, 1714, 1777, 1836
RsaNI GTAC 7 cut(s) 145, 646, 829, 877, 1713, 1776, 1835
RseI CAYNNNNRTG 1 cut(s) 816
SacII CCGCGG 1 cut(s) 369
SalI GTCGAC 1 cut(s) 595
SapI GCTCTTC 1 cut(s) 536
SaqAI TTAA 6 cut(s) 707, 732, 905, 1017, 1731, 2255
Sau3AI GATC 4 cut(s) 303, 631, 1238, 2113
ScaI AGTACT 1 cut(s) 878
SchI GAGTC 4 cut(s) 188, 443, 1120, 2226
ScrFI CCNGG 5 cut(s) 131, 334, 448, 1462, 1752
SduI GDGCHC 1 cut(s) 1195
SfaNI GCATC 7 cut(s) 783, 913, 1258, 1284, 2128, 2155, 2193
SfcI CTRYAG 5 cut(s) 797, 1380, 1584, 2133, 2144
SfoI GGCGCC 1 cut(s) 247
Sfr303I CCGCGG 1 cut(s) 369
SgrBI CCGCGG 1 cut(s) 369
SinI GGWCC 8 cut(s) 27, 327, 462, 1003, 1095, 1427, 1473, 2007
SmiMI CAYNNNNRTG 1 cut(s) 816
SmlI CTYRAG 5 cut(s) 750, 1280, 1298, 1576, 1903
SmoI CTYRAG 5 cut(s) 750, 1280, 1298, 1576, 1903
SphI GCATGC 2 cut(s) 1652, 2107
SseBI AGGCCT 2 cut(s) 512, 1582
SspDI GGCGCC 1 cut(s) 245
SspI AATATT 1 cut(s) 1030
SspMI CTAG 1 cut(s) 699
StuI AGGCCT 2 cut(s) 512, 1582
StyD4I CCNGG 5 cut(s) 129, 332, 446, 1460, 1750
StyI CCWWGG 3 cut(s) 228, 844, 1445
TaiI ACGT 3 cut(s) 349, 410, 1118
TaqI TCGA 6 cut(s) 264, 404, 596, 789, 1410, 2380
TaqII GACCGA 1 cut(s) 123
TatI WGTACW 1 cut(s) 876
TfiI GAWTC 5 cut(s) 4, 89, 101, 1745, 2382
Tru1I TTAA 6 cut(s) 707, 732, 905, 1017, 1731, 2255
Tru9I TTAA 6 cut(s) 707, 732, 905, 1017, 1731, 2255
TscAI CASTG 6 cut(s) 553, 1774, 1951, 1959, 1995, 2037
TseFI GTSAC 6 cut(s) 342, 347, 383, 619, 1064, 1600
TseI GCWGC 8 cut(s) 395, 796, 799, 1079, 1382, 1608, 1624, 2018
Tsp45I GTSAC 6 cut(s) 342, 347, 383, 619, 1064, 1600
TspRI CASTG 6 cut(s) 553, 1774, 1951, 1959, 1995, 2037
Tth111I GACNNNGTC 1 cut(s) 1099
Van91I CCANNNNNTGG 1 cut(s) 1931
VpaK11BI GGWCC 8 cut(s) 27, 327, 462, 1003, 1095, 1427, 1473, 2007
XagI CCTNNNNNAGG 1 cut(s) 227
XapI RAATTY 2 cut(s) 1403, 1725
XceI RCATGY 3 cut(s) 1633, 1652, 2107
XmiI GTMKAC 2 cut(s) 159, 596
XmnI GAANNNNTTC 1 cut(s) 1152
XspI CTAG 1 cut(s) 699
ZrmI AGTACT 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.