RLG00000006392

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009465
Physical Location & Seq
Forward (+)
6716810 .. 6724230
7421 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006392

Sequence Viewer

Length: 2328 bp
ATGGATCCGACCGGCACCCCTCGCCGGACCCGAAATATCCCGCCGAAACCCAACCTCTACTCCACTGTAGTCGTCCATGGCGACGACGATTCCACCTCCGAATCCAACCGAGACCGGCGGCGCGGCAACCAGGAACCCGACCCGTACGCCACATTCGTATACAAGAACAATGCTCACGACGAAGACGACGAAGACGACGACGACGACTCCTCCCTCCCTCCCCTCCTCAAACGCCTCCCCAAGGACTTCGGCGGCGGCGCCTCCCTCAACTACTTCGACGACGAGGACGACAACGGCGGCGATTTCGGCACCGTGATCGTCAAGAGCGACCGCAATCGGACCACCAGGAGGTCGTCACGTGACTGGGGGAGCCGAGCCGCGGAGGCGGACGACGGTGACGGCGGAGATGCGTTCTCGACGTTTGTGATGAGGCCGAGTGAGAGAGAGTCGTCGCTGACCGGGACGGTGAGGAGGACCAGCGGCGGCAGTTCCACGATGAGCAGGGCGGTGGCGAGTATGCAGGCCTCCGGCGACGGGATGGGGAAGCAGAGGAAGAGCAGTGCCGCCTCCTCTCAAAACGAGGAGGCTCGCCAGAATATGAAAATGTCGGCCAGTTCCATACCGGAAAGCGTCACCAGAGAAGATCCGACTACAAAGTACGAGTTGCTCAATGAGCTTGGGAAGGGGTCTTATGGTGCGGTTTATAAAGCTAGGGACTTAAAGACTTCCGAGCTGGTGGCAATTAAAGTCATTTCATTATCTCAAGGGGAGGAAGGGTATGAAGAGATTTGTGGTGAAATTGAGATGCTGCAGCAATGCAGTCATCCGAATGTCGTTCGGTACTTGGGGAGCTACCAAGGAGAAGAGTATCTTTGGATTGTGATGGAGTACTGTGGCGGTGGAAGTGTTGCTGACTTAATGAATGTTACCGAGGATGCTTTGGAGGAGTATCAAATAGCTTACATTTGCAGGGAAGCATTGAAGGGCCTTGCTTATTTGCACTCAATTTTCAAGGTCCACAGAGATATTAAGGGTGGTAATATTCTGTTAACTGAACAAGGAGATGTCAAGTTGGGTGACTTTGGGGTTGCAGCACAGCTTACAAGGACCATGTCAAAGCGCAATACGTTCATTGGGACTCCACATTGGATGGCTCCAGAAGTTATTCAGGAAAGCCGTTATGATGGAAAGGTGGATGTATGGGCTCTTGGTGTTTCTGCAATTGAAATGGCTGAGGGACTTCCTCCAAGATCTGCGGTGCATCCAATGAGGGTTCTATTCATGATATCCCTTGAGCCAGCTCCAATGCTTGAGGATAAAGAAAAATGGTCGCACAAATTCATCGAAAAATGCAAATGTGGTCCCTCTGCAATGGTGCCAAGGATTGAGAATGCCAGGCAAATTAGGACCTCTATGGCTTTGCAAGCACAAAATGAAGCTCCAGATGAACAGGAAGACGATACACTTGGAGCTCCCAAAGTGAATGAAGATTATGGAGATACCGTTCCTTCAAGGCCTCTAGGGGCAAATGAAGTGACAGGCAGCACATTGACGAAGCAGCACATGTCTGGTATTGGGGAGCATGCAGAGGAAGGTAATTTTGGCACCGTAATAATTCATGGTGGAGATGAAAGAGATGAGACTGGTACTCAGACACAAATTTTTAATTCCAAAGAAGATTCACCTGCCTCTGGACTTGGCCCTTCCACTGCTGGTACAGTAGGTAAATCAAGCGAAGTCAGGGTGGGAGACGAAAATAACACCTCAGTTGGAGTACGAAGTACAGTTACACAGACCAAACCAGCATTTTCTCTTTCAACCTTTACCTCTTCTGAGCCGAACTTGAGAAAATGCAAATCTCAACCACAGGTTGGAAGTCAAAGCGGTGGCACAGTGAAGAATGAAACTTTCAGCCGGAAAGCTTTTGCAGTGCAAGATAAGCTTTGGTCCATATATGCAGCTGGTAATACAGTGCCTATTCCATTTTTGAGGGCAACAGATATATCCCCCATTGCTCTCTTATCAGATAATGTTGTTGGAGGCATGCAACGAGATCATAGTGGAACTGGTGCTATCGATGCACTACAGGAGCTTTTTACTGGCGATGCACAGTCTAAAAAGGGACGAAGAGGGCAAAATGAGATGCCCCTTCCTCCAAGTGTTTACAAAAGACTCTTTGCAAGTCCAACTCTATTAAATCTCACACAAGCTTTAGCCTACCACAAGATGTGCTATGAAGATATGCCTCTCCAAGAATTGCAAGCAACTCAGGAACAACAGACTATTCAAAATCTTTGCGATACTCTAAGAACCATTCTTCGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006928 GO:0006950 GO:0006996 GO:0007010 GO:0007154 GO:0007165 GO:0007264 GO:0007265 GO:0007266 GO:0007346 GO:0008150 GO:0008152 GO:0008219 GO:0008283 GO:0009893 GO:0009966 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0012501 GO:0016043 GO:0016301 GO:0016310 GO:0016477 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017048 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0023014 GO:0023051 GO:0023052 GO:0030029 GO:0030036 GO:0031098 GO:0031267 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0040011 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043408 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048365 GO:0048518 GO:0048522 GO:0048583 GO:0048870 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051020 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051674 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1902531
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

776

Amino Acids

84.79

Weight (kDa)

5.09

Isoelectric Point (pI)

50.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 220 - 421 4.8e-61 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 222 - 431 9.7e-46 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 293 - 371 1.3e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
AarI CACCTGC 1 cut(s) 1693
Acc36I ACCTGC 1 cut(s) 1693
AccB1I GGYRCC 5 cut(s) 14, 257, 308, 1375, 1604
AccB7I CCANNNNNTGG 1 cut(s) 1871
AccI GTMKAC 1 cut(s) 159
AccII CGCG 2 cut(s) 123, 380
AclWI GGATC 2 cut(s) 12, 638
AcoI YGGCCR 1 cut(s) 609
AcsI RAATTY 2 cut(s) 1337, 1659
AcvI CACGTG 1 cut(s) 359
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 8 cut(s) 146, 659, 842, 890, 1648, 1717, 1776, 1783
AfiI CCNNNNNNNGG 7 cut(s) 24, 241, 348, 379, 534, 1691, 1871
AflIII ACRYGT 1 cut(s) 1563
AgsI TTSAA 6 cut(s) 982, 1012, 1226, 1512, 1818, 2288
AjnI CCWGG 3 cut(s) 129, 344, 1394
AjuI GAANNNNNNNTTGG 2 cut(s) 1584, 1616
AloI GAACNNNNNNTCC 2 cut(s) 1488, 1520
Alw21I GWGCWC 1 cut(s) 1474
Alw26I GTCTC 3 cut(s) 105, 1634, 1743
AlwI GGATC 2 cut(s) 12, 638
AoxI GGCC 6 cut(s) 431, 522, 609, 985, 1514, 1699
ApeKI GCWGC 6 cut(s) 808, 811, 1091, 1542, 1558, 1958
ApoI RAATTY 2 cut(s) 1337, 1659
Asp700I GAANNNNTTC 1 cut(s) 1164
AspLEI GCGC 3 cut(s) 123, 260, 1122
AsuC2I CCSGG 1 cut(s) 460
AsuHPI GGTGA 6 cut(s) 407, 478, 625, 806, 1088, 1674
AvaII GGWCC 8 cut(s) 27, 339, 474, 1015, 1107, 1361, 1407, 1947
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 5 cut(s) 14, 257, 308, 1375, 1604
BanII GRGCYC 2 cut(s) 1207, 1474
BarI GAAGNNNNNNTAC 2 cut(s) 1771, 1803
BbrPI CACGTG 1 cut(s) 359
BbsI GAAGAC 3 cut(s) 189, 198, 1461
Bbv12I GWGCWC 1 cut(s) 1474
BbvCI CCTCAGC 1 cut(s) 1233
BbvI GCAGC 6 cut(s) 795, 823, 1103, 1554, 1570, 1970
BccI CCATC 4 cut(s) 532, 877, 1144, 1178
BceAI ACGGC 3 cut(s) 310, 415, 1161
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BciT130I CCWGG 3 cut(s) 131, 346, 1396
BcnI CCSGG 1 cut(s) 460
BcoDI GTCTC 3 cut(s) 105, 1634, 1743
BfaI CTAG 2 cut(s) 711, 1520
BfmI CTRYAG 3 cut(s) 66, 809, 2084
BfoI RGCGCY 1 cut(s) 261
BfuAI ACCTGC 1 cut(s) 1693
BglI GCCNNNNNGGC 1 cut(s) 383
BglII AGATCT 1 cut(s) 1250
BmcAI AGTACT 1 cut(s) 890
Bme1390I CCNGG 4 cut(s) 131, 346, 460, 1396
Bme18I GGWCC 8 cut(s) 27, 339, 474, 1015, 1107, 1361, 1407, 1947
BmrFI CCNGG 4 cut(s) 131, 346, 460, 1396
BmrI ACTGGG 1 cut(s) 373
BmsI GCATC 7 cut(s) 397, 795, 925, 1270, 2068, 2095, 2133
BmuI ACTGGG 1 cut(s) 373
BpiI GAAGAC 3 cut(s) 189, 198, 1461
BpmI CTGGAG 2 cut(s) 1140, 1425
Bpu10I CCTNAGC 1 cut(s) 1233
BpuEI CTTGAG 4 cut(s) 747, 1313, 1331, 1864
BpuMI CCSGG 1 cut(s) 460
Bsa29I ATCGAT 1 cut(s) 2076
BsaAI YACGTR 1 cut(s) 359
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 6 cut(s) 76, 240, 378, 856, 930, 1379
BsaWI WCCGGW 1 cut(s) 622
Bsc4I CCNNNNNNNGG 7 cut(s) 24, 241, 348, 379, 534, 1691, 1871
Bse118I RCCGGY 2 cut(s) 11, 114
Bse1I ACTGG 5 cut(s) 368, 612, 1648, 2071, 2104
Bse3DI GCAATG 3 cut(s) 821, 1377, 2010
BseBI CCWGG 3 cut(s) 131, 346, 1396
BseCI ATCGAT 1 cut(s) 2076
BseDI CCNNGG 6 cut(s) 76, 240, 378, 856, 930, 1379
BseGI GGATG 6 cut(s) 543, 823, 940, 1155, 1201, 1261
BseLI CCNNNNNNNGG 7 cut(s) 24, 241, 348, 379, 534, 1691, 1871
BseMI GCAATG 3 cut(s) 821, 1377, 2010
BseMII CTCAG 5 cut(s) 1224, 1664, 1779, 1824, 2282
BseNI ACTGG 5 cut(s) 368, 612, 1648, 2071, 2104
BseRI GAGGAG 6 cut(s) 199, 215, 484, 559, 596, 959
BseXI GCAGC 6 cut(s) 795, 823, 1103, 1554, 1570, 1970
Bsh1236I CGCG 2 cut(s) 123, 380
Bsh1285I CGRYCG 2 cut(s) 12, 331
BshFI GGCC 6 cut(s) 433, 524, 611, 987, 1516, 1701
BshNI GGYRCC 5 cut(s) 14, 257, 308, 1375, 1604
BshVI ATCGAT 1 cut(s) 2076
BsiEI CGRYCG 2 cut(s) 12, 331
BsiHKAI GWGCWC 1 cut(s) 1474
BsiSI CCGG 7 cut(s) 12, 25, 115, 459, 528, 623, 1915
BsiWI CGTACG 1 cut(s) 144
BslFI GGGAC 6 cut(s) 475, 728, 1150, 1251, 1347, 2136
BslI CCNNNNNNNGG 7 cut(s) 24, 241, 348, 379, 534, 1691, 1871
BsmAI GTCTC 3 cut(s) 105, 1634, 1743
BsmBI CGTCTC 1 cut(s) 1743
BsmFI GGGAC 6 cut(s) 475, 728, 1150, 1251, 1347, 2136
BsmI GAATGC 1 cut(s) 1396
BsnI GGCC 6 cut(s) 433, 524, 611, 987, 1516, 1701
Bso31I GGTCTC 1 cut(s) 105
Bsp1286I GDGCHC 2 cut(s) 1207, 1474
Bsp143I GATC 5 cut(s) 4, 315, 643, 1250, 2053
Bsp19I CCATGG 1 cut(s) 76
BspANI GGCC 6 cut(s) 433, 524, 611, 987, 1516, 1701
BspCNI CTCAG 5 cut(s) 1225, 1663, 1778, 1825, 2281
BspDI ATCGAT 1 cut(s) 2076
BspFNI CGCG 2 cut(s) 123, 380
BspHI TCATGA 1 cut(s) 1281
BspMAI CTGCAG 1 cut(s) 813
BspMI ACCTGC 1 cut(s) 1693
BspPI GGATC 2 cut(s) 12, 638
BspQI GCTCTTC 1 cut(s) 548
BspT107I GGYRCC 5 cut(s) 14, 257, 308, 1375, 1604
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 3 cut(s) 821, 1377, 2010
BsrFI RCCGGY 2 cut(s) 11, 114
BsrI ACTGG 5 cut(s) 368, 612, 1648, 2071, 2104
BssAI RCCGGY 2 cut(s) 11, 114
BssECI CCNNGG 6 cut(s) 76, 240, 378, 856, 930, 1379
BssMI GATC 5 cut(s) 4, 315, 643, 1250, 2053
BssNAI GTATAC 1 cut(s) 160
BssNI GRCGYC 1 cut(s) 258
BssT1I CCWWGG 4 cut(s) 76, 240, 856, 1379
Bst1107I GTATAC 1 cut(s) 160
Bst2UI CCWGG 3 cut(s) 131, 346, 1396
Bst6I CTCTTC 5 cut(s) 548, 777, 858, 1834, 2122
BstACI GRCGYC 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 359
BstC8I GCNNGC 7 cut(s) 522, 589, 1299, 1425, 1584, 2045, 2262
BstDEI CTNAG 6 cut(s) 1233, 1650, 1765, 1833, 2268, 2306
BstDSI CCRYGG 2 cut(s) 76, 378
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 6 cut(s) 543, 823, 940, 1155, 1201, 1261
BstFNI CGCG 2 cut(s) 123, 380
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 3 cut(s) 123, 260, 1122
BstKTI GATC 5 cut(s) 7, 318, 646, 1253, 2056
BstMAI GTCTC 3 cut(s) 105, 1634, 1743
BstMBI GATC 5 cut(s) 4, 315, 643, 1250, 2053
BstMCI CGRYCG 2 cut(s) 12, 331
BstMWI GCNNNNNNNGC 7 cut(s) 21, 306, 383, 673, 1424, 1939, 2078
BstNI CCWGG 3 cut(s) 131, 346, 1396
BstNSI RCATGY 3 cut(s) 1567, 1586, 2047
BstSCI CCNGG 4 cut(s) 129, 344, 458, 1394
BstSFI CTRYAG 3 cut(s) 66, 809, 2084
BstUI CGCG 2 cut(s) 123, 380
BstV1I GCAGC 6 cut(s) 795, 823, 1103, 1554, 1570, 1970
BstV2I GAAGAC 3 cut(s) 189, 198, 1461
BstX2I RGATCY 3 cut(s) 4, 643, 1250
BstYI RGATCY 3 cut(s) 4, 643, 1250
BstZ17I GTATAC 1 cut(s) 160
Bsu15I ATCGAT 1 cut(s) 2076
BsuRI GGCC 6 cut(s) 433, 524, 611, 987, 1516, 1701
BsuTUI ATCGAT 1 cut(s) 2076
BtgI CCRYGG 2 cut(s) 76, 378
BtgZI GCGATG 1 cut(s) 2118
BtsCI GGATG 6 cut(s) 543, 823, 940, 1155, 1201, 1261
BtsI GCAGTG 3 cut(s) 565, 1707, 1935
BtsIMutI CAGTG 6 cut(s) 63, 565, 1707, 1899, 1935, 1977
BveI ACCTGC 1 cut(s) 1693
Cac8I GCNNGC 7 cut(s) 522, 589, 1299, 1425, 1584, 2045, 2262
CciI TCATGA 1 cut(s) 1281
CfoI GCGC 3 cut(s) 123, 260, 1122
Cfr10I RCCGGY 2 cut(s) 11, 114
Cfr42I CCGCGG 1 cut(s) 381
ClaI ATCGAT 1 cut(s) 2076
CseI GACGC 1 cut(s) 619
Csp6I GTAC 8 cut(s) 145, 658, 841, 889, 1647, 1716, 1775, 1782
CviAII CATG 7 cut(s) 77, 1111, 1282, 1564, 1583, 1619, 2044
CviQI GTAC 8 cut(s) 145, 658, 841, 889, 1647, 1716, 1775, 1782
DdeI CTNAG 6 cut(s) 1233, 1650, 1765, 1833, 2268, 2306
DinI GGCGCC 1 cut(s) 259
DpnI GATC 5 cut(s) 6, 317, 645, 1252, 2055
DpnII GATC 5 cut(s) 4, 315, 643, 1250, 2053
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 5 cut(s) 548, 777, 858, 1834, 2122
EarI CTCTTC 5 cut(s) 548, 777, 858, 1834, 2122
EciI GGCGGA 2 cut(s) 401, 417
Ecl136II GAGCTC 1 cut(s) 1472
Eco130I CCWWGG 4 cut(s) 76, 240, 856, 1379
Eco147I AGGCCT 2 cut(s) 524, 1516
Eco24I GRGCYC 2 cut(s) 1207, 1474
Eco31I GGTCTC 1 cut(s) 105
Eco32I GATATC 1 cut(s) 1287
Eco47I GGWCC 8 cut(s) 27, 339, 474, 1015, 1107, 1361, 1407, 1947
Eco53kI GAGCTC 1 cut(s) 1472
Eco72I CACGTG 1 cut(s) 359
EcoICRI GAGCTC 1 cut(s) 1472
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoO109I RGGNCCY 2 cut(s) 985, 1407
EcoRII CCWGG 3 cut(s) 129, 344, 1394
EcoRV GATATC 1 cut(s) 1287
EcoT14I CCWWGG 4 cut(s) 76, 240, 856, 1379
EcoT38I GRGCYC 2 cut(s) 1207, 1474
EgeI GGCGCC 1 cut(s) 259
EheI GGCGCC 1 cut(s) 259
ErhI CCWWGG 4 cut(s) 76, 240, 856, 1379
Esp3I CGTCTC 1 cut(s) 1743
FaeI CATG 7 cut(s) 80, 1114, 1285, 1567, 1586, 1622, 2047
FalI AAGNNNNNCTT 4 cut(s) 855, 887, 1926, 1958
FaqI GGGAC 6 cut(s) 475, 728, 1150, 1251, 1347, 2136
FatI CATG 7 cut(s) 76, 1110, 1281, 1563, 1582, 1618, 2043
FauI CCCGC 1 cut(s) 48
FblI GTMKAC 1 cut(s) 159
FokI GGATG 6 cut(s) 550, 810, 947, 1162, 1208, 1248
FriOI GRGCYC 2 cut(s) 1207, 1474
FspBI CTAG 2 cut(s) 711, 1520
GlaI GCGC 3 cut(s) 122, 259, 1121
GsuI CTGGAG 2 cut(s) 1140, 1425
HaeII RGCGCY 1 cut(s) 261
HaeIII GGCC 6 cut(s) 433, 524, 611, 987, 1516, 1701
HapII CCGG 7 cut(s) 12, 25, 115, 459, 528, 623, 1915
HgaI GACGC 1 cut(s) 619
HhaI GCGC 3 cut(s) 123, 260, 1122
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 7 cut(s) 80, 1114, 1285, 1567, 1586, 1622, 2047
Hin6I GCGC 3 cut(s) 121, 258, 1120
HinP1I GCGC 3 cut(s) 121, 258, 1120
HincII GTYRAC 1 cut(s) 1050
HindII GTYRAC 1 cut(s) 1050
HindIII AAGCTT 3 cut(s) 1920, 1940, 2208
HinfI GANTC 7 cut(s) 89, 101, 206, 446, 1138, 1679, 2172
HpaI GTTAAC 1 cut(s) 1050
HpaII CCGG 7 cut(s) 12, 25, 115, 459, 528, 623, 1915
HphI GGTGA 6 cut(s) 407, 478, 625, 806, 1088, 1674
Hpy166II GTNNAC 4 cut(s) 160, 1018, 1050, 2164
Hpy188I TCNGA 9 cut(s) 9, 100, 339, 648, 730, 828, 1653, 1834, 2026
Hpy188III TCNNGA 9 cut(s) 176, 322, 415, 1157, 1169, 1282, 1442, 1692, 2270
Hpy8I GTNNAC 4 cut(s) 160, 1018, 1050, 2164
HpyAV CCTTC 7 cut(s) 676, 767, 976, 1518, 1586, 1713, 2159
HpyCH4IV ACGT 3 cut(s) 358, 419, 1127
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 306, 383, 673, 1424, 1939, 2078
HpyF3I CTNAG 6 cut(s) 1233, 1650, 1765, 1833, 2268, 2306
HpySE526I ACGT 3 cut(s) 358, 419, 1127
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 7 cut(s) 80, 1114, 1285, 1567, 1586, 1622, 2047
HspAI GCGC 3 cut(s) 121, 258, 1120
KasI GGCGCC 1 cut(s) 257
KspAI GTTAAC 1 cut(s) 1050
KspI CCGCGG 1 cut(s) 381
Kzo9I GATC 5 cut(s) 4, 315, 643, 1250, 2053
LguI GCTCTTC 1 cut(s) 548
LmnI GCTCC 9 cut(s) 369, 849, 1159, 1306, 1444, 1469, 1477, 1579, 2089
Lsp1109I GCAGC 6 cut(s) 795, 823, 1103, 1554, 1570, 1970
LweI GCATC 7 cut(s) 397, 795, 925, 1270, 2068, 2095, 2133
MaeI CTAG 2 cut(s) 711, 1520
MaeII ACGT 3 cut(s) 358, 419, 1127
MaeIII GTNAC 8 cut(s) 354, 359, 395, 631, 925, 1076, 1534, 1786
MalI GATC 5 cut(s) 6, 317, 645, 1252, 2055
MboI GATC 5 cut(s) 4, 315, 643, 1250, 2053
MfeI CAATTG 1 cut(s) 1221
MflI RGATCY 3 cut(s) 4, 643, 1250
MhlI GDGCHC 2 cut(s) 1207, 1474
Mly113I GGCGCC 1 cut(s) 258
MlyI GAGTC 4 cut(s) 200, 455, 1132, 2166
MmeI TCCRAC 7 cut(s) 32, 129, 671, 1750, 1852, 2017, 2210
MroXI GAANNNNTTC 1 cut(s) 1164
MseI TTAA 7 cut(s) 719, 744, 917, 1029, 1049, 1665, 2195
MslI CAYNNNNRTG 1 cut(s) 828
MspA1I CMGCKG 3 cut(s) 380, 480, 1961
MspI CCGG 7 cut(s) 12, 25, 115, 459, 528, 623, 1915
MspR9I CCNGG 4 cut(s) 131, 346, 460, 1396
MunI CAATTG 1 cut(s) 1221
Mva1269I GAATGC 1 cut(s) 1396
MvaI CCWGG 3 cut(s) 131, 346, 1396
MvnI CGCG 2 cut(s) 123, 380
MwoI GCNNNNNNNGC 7 cut(s) 21, 306, 383, 673, 1424, 1939, 2078
NarI GGCGCC 1 cut(s) 258
NciI CCSGG 1 cut(s) 460
NcoI CCATGG 1 cut(s) 76
NdeII GATC 5 cut(s) 4, 315, 643, 1250, 2053
NlaIII CATG 7 cut(s) 80, 1114, 1285, 1567, 1586, 1622, 2047
NmeAIII GCCGAG 2 cut(s) 398, 459
NmuCI GTSAC 6 cut(s) 354, 359, 395, 631, 1076, 1534
NspI RCATGY 3 cut(s) 1567, 1586, 2047
PaeI GCATGC 2 cut(s) 1586, 2047
PagI TCATGA 1 cut(s) 1281
PaqCI CACCTGC 1 cut(s) 1693
PceI AGGCCT 2 cut(s) 524, 1516
PciI ACATGT 1 cut(s) 1563
PciSI GCTCTTC 1 cut(s) 548
PctI GAATGC 1 cut(s) 1396
PdmI GAANNNNTTC 1 cut(s) 1164
PfeI GAWTC 3 cut(s) 89, 101, 1679
Pfl23II CGTACG 1 cut(s) 144
PflFI GACNNNGTC 1 cut(s) 1111
PflMI CCANNNNNTGG 1 cut(s) 1871
PleI GAGTC 4 cut(s) 200, 454, 1132, 2166
PluTI GGCGCC 1 cut(s) 261
PmaCI CACGTG 1 cut(s) 359
PmlI CACGTG 1 cut(s) 359
PpsI GAGTC 4 cut(s) 200, 454, 1132, 2166
Ppu21I YACGTR 1 cut(s) 359
PpuMI RGGWCCY 1 cut(s) 1407
PscI ACATGT 1 cut(s) 1563
PsiI TTATAA 1 cut(s) 705
Psp124BI GAGCTC 1 cut(s) 1474
Psp5II RGGWCCY 1 cut(s) 1407
Psp6I CCWGG 3 cut(s) 129, 344, 1394
PspCI CACGTG 1 cut(s) 359
PspGI CCWGG 3 cut(s) 129, 344, 1394
PspLI CGTACG 1 cut(s) 144
PspPPI RGGWCCY 1 cut(s) 1407
PstI CTGCAG 1 cut(s) 813
PsuI RGATCY 3 cut(s) 4, 643, 1250
PsyI GACNNNGTC 1 cut(s) 1111
PvuII CAGCTG 1 cut(s) 1961
RsaI GTAC 8 cut(s) 146, 659, 842, 890, 1648, 1717, 1776, 1783
RsaNI GTAC 8 cut(s) 145, 658, 841, 889, 1647, 1716, 1775, 1782
RseI CAYNNNNRTG 1 cut(s) 828
SacI GAGCTC 1 cut(s) 1474
SacII CCGCGG 1 cut(s) 381
SapI GCTCTTC 1 cut(s) 548
SaqAI TTAA 7 cut(s) 719, 744, 917, 1029, 1049, 1665, 2195
Sau3AI GATC 5 cut(s) 4, 315, 643, 1250, 2053
ScaI AGTACT 1 cut(s) 890
SchI GAGTC 4 cut(s) 200, 455, 1132, 2166
ScrFI CCNGG 4 cut(s) 131, 346, 460, 1396
SduI GDGCHC 2 cut(s) 1207, 1474
SfaNI GCATC 7 cut(s) 397, 795, 925, 1270, 2068, 2095, 2133
SfcI CTRYAG 3 cut(s) 66, 809, 2084
SfoI GGCGCC 1 cut(s) 259
Sfr303I CCGCGG 1 cut(s) 381
SgrBI CCGCGG 1 cut(s) 381
SinI GGWCC 8 cut(s) 27, 339, 474, 1015, 1107, 1361, 1407, 1947
SmiMI CAYNNNNRTG 1 cut(s) 828
SmlI CTYRAG 4 cut(s) 762, 1292, 1310, 1843
SmoI CTYRAG 4 cut(s) 762, 1292, 1310, 1843
SphI GCATGC 2 cut(s) 1586, 2047
SseBI AGGCCT 2 cut(s) 524, 1516
SspDI GGCGCC 1 cut(s) 257
SspI AATATT 1 cut(s) 1042
SspMI CTAG 2 cut(s) 711, 1520
SstI GAGCTC 1 cut(s) 1474
StuI AGGCCT 2 cut(s) 524, 1516
StyD4I CCNGG 4 cut(s) 129, 344, 458, 1394
StyI CCWWGG 4 cut(s) 76, 240, 856, 1379
TaiI ACGT 3 cut(s) 361, 422, 1130
TaqI TCGA 5 cut(s) 276, 416, 1344, 2076, 2320
TatI WGTACW 2 cut(s) 888, 1781
TauI GCSGC 9 cut(s) 121, 126, 255, 258, 300, 380, 483, 486, 566
TfiI GAWTC 3 cut(s) 89, 101, 1679
Tru1I TTAA 7 cut(s) 719, 744, 917, 1029, 1049, 1665, 2195
Tru9I TTAA 7 cut(s) 719, 744, 917, 1029, 1049, 1665, 2195
TscAI CASTG 6 cut(s) 70, 565, 1714, 1899, 1935, 1977
TseFI GTSAC 6 cut(s) 354, 359, 395, 631, 1076, 1534
TseI GCWGC 6 cut(s) 808, 811, 1091, 1542, 1558, 1958
Tsp45I GTSAC 6 cut(s) 354, 359, 395, 631, 1076, 1534
TspRI CASTG 6 cut(s) 70, 565, 1714, 1899, 1935, 1977
Tth111I GACNNNGTC 1 cut(s) 1111
Van91I CCANNNNNTGG 1 cut(s) 1871
VpaK11BI GGWCC 8 cut(s) 27, 339, 474, 1015, 1107, 1361, 1407, 1947
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 2 cut(s) 1337, 1659
XceI RCATGY 3 cut(s) 1567, 1586, 2047
XmiI GTMKAC 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 1164
XspI CTAG 2 cut(s) 711, 1520
ZrmI AGTACT 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.