FvH4_4g31160
MADS Family

MADS-box transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002890
Physical Location & Seq
Forward (+)
30420522 .. 30424484
3963 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31160.t2

Sequence Viewer

Length: 1059 bp
ATGGGAAGGGTAAAGCTAAAGATTAAGAGGTTGGAGAATACAAATGGGCGTCAAGCAACTTATGCGAAAAGGAAGCATGGGATCATGAAGAAGGCTAATGAATTATCCATACTATGTGATATAGACATTGTCCTTCTTATGTTTTCGCCCACTGGGAAGCCTTCTTTATGCAGTGGAAAACGCAGTAGCATTGAAGAGGTTATAGCAAAGTTTTCTCAACTAACTCCACAAGAAAGAGCTAAAAGGAAACTGGAAAGCCTCGAAGCACTGAAGAAAACATTCAGGAAGTTGGACCATGATGTGAACATACAAGAATTTTTGGGTACAAGTTCTCAAACAGTCGAGGACTTGAGTAACCGATCTAGGTTGCTGCAAACCCAAATTTCCGAAATAGAGAAGAGATTAAGCTGTTGGACGAACCCTGATAAGGTCAACAGTGTAGATCAATTGGGGCAAATGGAAGATTCAATTAGAGAATCGCTTAACCAGATTCGAACACATAAGGAAAATTTACAAAAACAACAACTAATGTCATTAGAATGCACCAGTCAGTTCCAAAATGGATTACATATACCCTTCAGCATGAGTACTGAGCATCAGCTCCAGCCTATGCCATGGATTCCTAGTAATGAAAACCGGCACATCGTTTTACCAGAGGACCCGACGTTGCTTCCTCATAGGGACATGGAATGTTCTGCAAGTTCCTCCTTTGGCAGTTATTCTGGTTACCTTGGGACAGGAAAAAGTCCAGAGATTTCCAACTCTGGTCAGGAAAACGGTATTCTAAACGAGTTAAGCAGAACTGCACCACTGAGGGTACAGCTGGGAGGGCAGTTTTCCTCCTACCTTCCATACAATCTCAATATGTTGACCGATACAAAATTCCAACAACCCGCAGAAATGAGTCCTCAAGAAAACCCTGTGGAATATCATGTTAATGGAAGCTTTGAAGCTCCTAGACCTGATTTTTTCGCTACCCAGCATAGCTGGGCATCTACATCTGGTCCCTGTGCTGTTACTATGTTTGACGAGCATTTATTTTCCCAGCAGACAAACTAA

Protein Analysis

353

Amino Acids

39.83

Weight (kDa)

6.8

Isoelectric Point (pI)

57.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 54 4.9e-20 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 894
AclWI GGATC 1 cut(s) 89
AcsI RAATTY 4 cut(s) 314, 381, 508, 881
AcuI CTGAAG 2 cut(s) 290, 562
AcyI GRCGYC 1 cut(s) 49
AfaI GTAC 3 cut(s) 325, 589, 819
AfiI CCNNNNNNNGG 1 cut(s) 427
AgsI TTSAA 3 cut(s) 194, 468, 950
AluBI AGCT 8 cut(s) 16, 239, 408, 601, 823, 945, 953, 987
AluI AGCT 8 cut(s) 16, 239, 408, 601, 823, 945, 953, 987
AlwI GGATC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 370
ApoI RAATTY 4 cut(s) 314, 381, 508, 881
Asp700I GAANNNNTTC 1 cut(s) 278
AspS9I GGNCC 3 cut(s) 292, 658, 1004
AsuII TTCGAA 1 cut(s) 493
AvaII GGWCC 3 cut(s) 292, 658, 1004
BbvI GCAGC 1 cut(s) 357
BfaI CTAG 3 cut(s) 363, 624, 957
BisI GCNGC 1 cut(s) 371
BlsI GCNGC 1 cut(s) 372
BmcAI AGTACT 1 cut(s) 589
Bme18I GGWCC 3 cut(s) 292, 658, 1004
BmgT120I GGNCC 3 cut(s) 292, 658, 1004
BmiI GGNNCC 2 cut(s) 660, 1006
BmrI ACTGGG 1 cut(s) 162
BmsI GCATC 2 cut(s) 604, 1001
BmuI ACTGGG 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 587
Bpu14I TTCGAA 1 cut(s) 493
BpuEI CTTGAG 2 cut(s) 370, 894
BsaHI GRCGYC 1 cut(s) 49
BsaJI CCNNGG 2 cut(s) 614, 730
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse118I RCCGGY 1 cut(s) 636
Bse1I ACTGG 3 cut(s) 157, 255, 546
BseDI CCNNGG 2 cut(s) 614, 730
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMII CTCAG 2 cut(s) 582, 803
BseNI ACTGG 3 cut(s) 157, 255, 546
BseXI GCAGC 1 cut(s) 357
BseYI CCCAGC 4 cut(s) 823, 978, 987, 1044
BsgI GTGCAG 1 cut(s) 789
BsiSI CCGG 1 cut(s) 637
BslFI GGGAC 3 cut(s) 695, 748, 990
BslI CCNNNNNNNGG 1 cut(s) 427
BsmFI GGGAC 3 cut(s) 695, 748, 990
BsmI GAATGC 1 cut(s) 545
Bsp119I TTCGAA 1 cut(s) 493
Bsp143I GATC 3 cut(s) 81, 359, 442
Bsp19I CCATGG 1 cut(s) 614
BspACI CCGC 1 cut(s) 894
BspCNI CTCAG 2 cut(s) 583, 804
BspHI TCATGA 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 660, 1006
BspPI GGATC 1 cut(s) 89
BspT104I TTCGAA 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 636
BsrI ACTGG 3 cut(s) 157, 255, 546
BssAI RCCGGY 1 cut(s) 636
BssECI CCNNGG 2 cut(s) 614, 730
BssMI GATC 3 cut(s) 81, 359, 442
BssNI GRCGYC 1 cut(s) 49
BssT1I CCWWGG 2 cut(s) 614, 730
Bst4CI ACNGT 3 cut(s) 340, 437, 779
Bst6I CTCTTC 2 cut(s) 189, 392
BstACI GRCGYC 1 cut(s) 49
BstAPI GCANNNNNTGC 1 cut(s) 62
BstBI TTCGAA 1 cut(s) 493
BstDEI CTNAG 2 cut(s) 591, 812
BstDSI CCRYGG 1 cut(s) 614
BstEII GGTNACC 1 cut(s) 725
BstKTI GATC 3 cut(s) 84, 362, 445
BstMBI GATC 3 cut(s) 81, 359, 442
BstMWI GCNNNNNNNGC 2 cut(s) 62, 829
BstPI GGTNACC 1 cut(s) 725
BstV1I GCAGC 1 cut(s) 357
BtgI CCRYGG 1 cut(s) 614
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 5 cut(s) 150, 178, 266, 442, 809
CciI TCATGA 1 cut(s) 84
Cfr10I RCCGGY 1 cut(s) 636
Cfr13I GGNCC 3 cut(s) 292, 658, 1004
CseI GACGC 1 cut(s) 38
Csp6I GTAC 3 cut(s) 324, 588, 818
CviAII CATG 7 cut(s) 77, 85, 296, 583, 615, 685, 932
CviQI GTAC 3 cut(s) 324, 588, 818
DdeI CTNAG 2 cut(s) 591, 812
DpnI GATC 3 cut(s) 83, 361, 444
DpnII GATC 3 cut(s) 81, 359, 442
Eam1104I CTCTTC 2 cut(s) 189, 392
EarI CTCTTC 2 cut(s) 189, 392
Eco130I CCWWGG 2 cut(s) 614, 730
Eco47I GGWCC 3 cut(s) 292, 658, 1004
Eco57I CTGAAG 2 cut(s) 290, 562
Eco91I GGTNACC 1 cut(s) 725
EcoO109I RGGNCCY 1 cut(s) 658
EcoO65I GGTNACC 1 cut(s) 725
EcoT14I CCWWGG 2 cut(s) 614, 730
ErhI CCWWGG 2 cut(s) 614, 730
FaeI CATG 7 cut(s) 80, 88, 299, 586, 618, 688, 935
FaqI GGGAC 3 cut(s) 695, 748, 990
FatI CATG 7 cut(s) 76, 84, 295, 582, 614, 684, 931
FauI CCCGC 1 cut(s) 901
Fnu4HI GCNGC 1 cut(s) 371
Fsp4HI GCNGC 1 cut(s) 371
FspBI CTAG 3 cut(s) 363, 624, 957
GluI GCNGC 1 cut(s) 371
GsaI CCCAGC 4 cut(s) 827, 982, 991, 1048
GsuI CTGGAG 1 cut(s) 587
HapII CCGG 1 cut(s) 637
HgaI GACGC 1 cut(s) 38
Hin1I GRCGYC 1 cut(s) 49
Hin1II CATG 7 cut(s) 80, 88, 299, 586, 618, 688, 935
HincII GTYRAC 2 cut(s) 433, 870
HindII GTYRAC 2 cut(s) 433, 870
HindIII AAGCTT 1 cut(s) 943
HinfI GANTC 5 cut(s) 464, 476, 490, 619, 904
HpaII CCGG 1 cut(s) 637
Hpy166II GTNNAC 3 cut(s) 304, 433, 870
Hpy188I TCNGA 1 cut(s) 388
Hpy188III TCNNGA 5 cut(s) 85, 283, 749, 770, 911
Hpy8I GTNNAC 3 cut(s) 304, 433, 870
Hpy99I CGWCG 1 cut(s) 667
HpyAV CCTTC 5 cut(s) 85, 143, 171, 586, 857
HpyCH4III ACNGT 3 cut(s) 340, 437, 779
HpyCH4IV ACGT 1 cut(s) 665
HpyCH4V TGCA 5 cut(s) 171, 373, 543, 698, 806
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 829
HpyF3I CTNAG 2 cut(s) 591, 812
HpySE526I ACGT 1 cut(s) 665
Hsp92I GRCGYC 1 cut(s) 49
Hsp92II CATG 7 cut(s) 80, 88, 299, 586, 618, 688, 935
Kzo9I GATC 3 cut(s) 81, 359, 442
LmnI GCTCC 2 cut(s) 606, 958
Lsp1109I GCAGC 1 cut(s) 357
LweI GCATC 2 cut(s) 604, 1001
MaeI CTAG 3 cut(s) 363, 624, 957
MaeII ACGT 1 cut(s) 665
MaeIII GTNAC 3 cut(s) 353, 725, 1015
MalI GATC 3 cut(s) 83, 361, 444
MboI GATC 3 cut(s) 81, 359, 442
MboII GAAGA 5 cut(s) 100, 206, 283, 409, 473
MfeI CAATTG 1 cut(s) 446
MluCI AATT 7 cut(s) 101, 314, 381, 446, 468, 508, 881
MlyI GAGTC 1 cut(s) 913
MmeI TCCRAC 5 cut(s) 12, 270, 392, 783, 910
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 5 cut(s) 24, 404, 483, 794, 936
MslI CAYNNNNRTG 2 cut(s) 538, 936
MspA1I CMGCKG 1 cut(s) 823
MspI CCGG 1 cut(s) 637
MunI CAATTG 1 cut(s) 446
Mva1269I GAATGC 1 cut(s) 545
MwoI GCNNNNNNNGC 2 cut(s) 62, 829
NcoI CCATGG 1 cut(s) 614
NdeII GATC 3 cut(s) 81, 359, 442
NlaIII CATG 7 cut(s) 80, 88, 299, 586, 618, 688, 935
NlaIV GGNNCC 2 cut(s) 660, 1006
NspV TTCGAA 1 cut(s) 493
PagI TCATGA 1 cut(s) 84
PctI GAATGC 1 cut(s) 545
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 4 cut(s) 464, 476, 490, 619
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 1 cut(s) 372
PleI GAGTC 1 cut(s) 912
PpsI GAGTC 1 cut(s) 912
PpuMI RGGWCCY 1 cut(s) 658
Psp5II RGGWCCY 1 cut(s) 658
PspEI GGTNACC 1 cut(s) 725
PspFI CCCAGC 4 cut(s) 823, 978, 987, 1044
PspN4I GGNNCC 2 cut(s) 660, 1006
PspPI GGNCC 3 cut(s) 292, 658, 1004
PspPPI RGGWCCY 1 cut(s) 658
PsyI GACNNNGTC 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 823
RsaI GTAC 3 cut(s) 325, 589, 819
RsaNI GTAC 3 cut(s) 324, 588, 818
RseI CAYNNNNRTG 2 cut(s) 538, 936
SaqAI TTAA 5 cut(s) 24, 404, 483, 794, 936
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 3 cut(s) 81, 359, 442
Sau96I GGNCC 3 cut(s) 292, 658, 1004
ScaI AGTACT 1 cut(s) 589
SchI GAGTC 1 cut(s) 913
SfaNI GCATC 2 cut(s) 604, 1001
SfuI TTCGAA 1 cut(s) 493
SinI GGWCC 3 cut(s) 292, 658, 1004
SmiMI CAYNNNNRTG 2 cut(s) 538, 936
SmlI CTYRAG 2 cut(s) 349, 909
SmoI CTYRAG 2 cut(s) 349, 909
Sse9I AATT 7 cut(s) 101, 314, 381, 446, 468, 508, 881
SsiI CCGC 1 cut(s) 894
SspMI CTAG 3 cut(s) 363, 624, 957
StyI CCWWGG 2 cut(s) 614, 730
TaaI ACNGT 3 cut(s) 340, 437, 779
TaiI ACGT 1 cut(s) 668
TaqI TCGA 3 cut(s) 261, 342, 493
TaqII GACCGA 1 cut(s) 887
TasI AATT 7 cut(s) 101, 314, 381, 446, 468, 508, 881
TatI WGTACW 1 cut(s) 587
TfiI GAWTC 4 cut(s) 464, 476, 490, 619
Tru1I TTAA 5 cut(s) 24, 404, 483, 794, 936
Tru9I TTAA 5 cut(s) 24, 404, 483, 794, 936
TscAI CASTG 5 cut(s) 157, 178, 273, 442, 816
TseI GCWGC 1 cut(s) 370
TspDTI ATGAA 3 cut(s) 101, 114, 645
TspRI CASTG 5 cut(s) 157, 178, 273, 442, 816
Tth111I GACNNNGTC 1 cut(s) 128
VpaK11BI GGWCC 3 cut(s) 292, 658, 1004
XapI RAATTY 4 cut(s) 314, 381, 508, 881
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 3 cut(s) 363, 624, 957
ZrmI AGTACT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.