MD13G1070300.v1.1
MADS Family

MADS-box transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002890
Physical Location & Seq
Reverse (-)
4925935 .. 4930553
4619 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1070300.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGGAAGGGTAAAGCTAAAGATTAAGAGGTTGGAGAACACAAATGGGCGTCAAGCAACTTATGCTAAAAGGAAGAATGGCATTATGAAAAAGGCTAATGAATTATCTATACTATGCGACATTGACATTATCCTTCTCATGTTTTCGCCTACCGGAAAGCCTTCTTTGTGCTGTGGCAAACGCAGTAGCATTGAAGACGTTATAGCAAAGTTTGCTCAACTCACTCCACAGGAAAGAGCTAAAAGGAAATTGGAAAGCCTCGAAGCACTGAAGAAAACATTCAAGAAGTTGGATCATGACGTTAATATACAAGAATTTTTGGGTACAAGTTCTCAAACGATTGAGGATCTGAGTAACCAGTCTAGTTTATTGCAAACCCAGCTTTCCGAAATAAAAAAGAGACTAAGCTGTTGGACTAGCCTTGATAAGATCAACAGTGTAGAACAGTTGGGGCATATGGAAGATTCACTTAGAGAATCGCTTAACCAGATTCGAACACATAAGGAAAATTTACAAAAGCAGCAGCTTATGTCAATAAAATGCACTAGTCAGTTCCAAAATGGGTTGCATATACCCTTCAGCATGAATGCTGAGCAGCAGCTCCAGCCTTTGTCATGGATTCCGACTAATGAAAGCCGACATATAGTTTTACCTGAGGACGCGACCTTGCTTCCCCATAGGGATATGGAATGCTCAGCAAGTTCCTCCTTTGGTAGTTATTCTGGTTACCTTGGCACAGGAAAAAGTCCAGAGATTTCTACTTCTGGACAAGAAAATGGTATTCTAAATGACTTAAGCAGAACCGCACCGCTGAATCTACAGCTGGGTGGGCACATTCCCTATCTTCCGTACAATCTCAATATGCTGACTGATACGAAATTCCAACCACCGGCAGAAATGAGTCCTCAAGAAAACCCTGTTGAGTATCAAGTTAATGGAAGCTTTGAAGCTCCTAGACCTGAGTTTTACCCCACTCAGCATAATTGGGCTTCTACATCTGGTCCTTGTGCAGTTACCATGTTTGACGAGCATTTATATTCCCAGACAAACTGA

Protein Analysis

351

Amino Acids

39.42

Weight (kDa)

7.11

Isoelectric Point (pI)

59.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 54 3.8e-20 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 662
AciI CCGC 2 cut(s) 804, 809
AclWI GGATC 2 cut(s) 300, 354
AcsI RAATTY 3 cut(s) 314, 508, 878
AcuI CTGAAG 2 cut(s) 290, 562
AcyI GRCGYC 1 cut(s) 49
AfaI GTAC 2 cut(s) 325, 851
AfiI CCNNNNNNNGG 1 cut(s) 889
AflII CTTAAG 1 cut(s) 793
AgsI TTSAA 3 cut(s) 194, 283, 947
AhlI ACTAGT 1 cut(s) 545
AluBI AGCT 9 cut(s) 16, 239, 382, 408, 526, 601, 823, 942, 950
AluI AGCT 9 cut(s) 16, 239, 382, 408, 526, 601, 823, 942, 950
Alw26I GTCTC 1 cut(s) 394
AlwI GGATC 2 cut(s) 300, 354
ApeKI GCWGC 4 cut(s) 520, 523, 595, 598
ApoI RAATTY 3 cut(s) 314, 508, 878
Asp700I GAANNNNTTC 2 cut(s) 160, 278
AspS9I GGNCC 1 cut(s) 1001
AsuII TTCGAA 1 cut(s) 493
AvaII GGWCC 1 cut(s) 1001
AxyI CCTNAGG 1 cut(s) 654
BaeGI GKGCMC 1 cut(s) 834
BbsI GAAGAC 1 cut(s) 201
BbvI GCAGC 4 cut(s) 532, 535, 607, 610
BcoDI GTCTC 1 cut(s) 394
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 4 cut(s) 363, 417, 546, 954
BfmI CTRYAG 1 cut(s) 818
BfrI CTTAAG 1 cut(s) 793
BisI GCNGC 4 cut(s) 521, 524, 596, 599
BlpI GCTNAGC 2 cut(s) 591, 694
BlsI GCNGC 4 cut(s) 522, 525, 597, 600
Bme18I GGWCC 1 cut(s) 1001
BmgT120I GGNCC 1 cut(s) 1001
BpiI GAAGAC 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 587
Bpu1102I GCTNAGC 2 cut(s) 591, 694
Bpu14I TTCGAA 1 cut(s) 493
BpuEI CTTGAG 1 cut(s) 891
BsaHI GRCGYC 1 cut(s) 49
BsaJI CCNNGG 1 cut(s) 730
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 1 cut(s) 889
Bse118I RCCGGY 1 cut(s) 889
Bse1I ACTGG 1 cut(s) 358
Bse21I CCTNAGG 1 cut(s) 654
BseDI CCNNGG 1 cut(s) 730
BseLI CCNNNNNNNGG 1 cut(s) 889
BseMII CTCAG 6 cut(s) 341, 582, 645, 708, 951, 989
BseNI ACTGG 1 cut(s) 358
BseSI GKGCMC 1 cut(s) 834
BseXI GCAGC 4 cut(s) 532, 535, 607, 610
BseYI CCCAGC 2 cut(s) 378, 823
BsgI GTGCAG 1 cut(s) 1029
Bsh1236I CGCG 1 cut(s) 662
BsiSI CCGG 2 cut(s) 153, 890
BslI CCNNNNNNNGG 1 cut(s) 889
BsmAI GTCTC 1 cut(s) 394
BsmI GAATGC 2 cut(s) 592, 695
Bsp119I TTCGAA 1 cut(s) 493
Bsp1286I GDGCHC 1 cut(s) 834
Bsp143I GATC 3 cut(s) 292, 346, 429
Bsp1720I GCTNAGC 2 cut(s) 591, 694
BspACI CCGC 2 cut(s) 804, 809
BspCNI CTCAG 6 cut(s) 342, 583, 646, 707, 952, 988
BspFNI CGCG 1 cut(s) 662
BspHI TCATGA 1 cut(s) 295
BspPI GGATC 2 cut(s) 300, 354
BspT104I TTCGAA 1 cut(s) 493
BspTI CTTAAG 1 cut(s) 793
BsrFI RCCGGY 1 cut(s) 889
BsrI ACTGG 1 cut(s) 358
BssAI RCCGGY 1 cut(s) 889
BssECI CCNNGG 1 cut(s) 730
BssMI GATC 3 cut(s) 292, 346, 429
BssNI GRCGYC 1 cut(s) 49
BssT1I CCWWGG 1 cut(s) 730
Bst4CI ACNGT 2 cut(s) 437, 447
BstACI GRCGYC 1 cut(s) 49
BstAFI CTTAAG 1 cut(s) 793
BstAPI GCANNNNNTGC 2 cut(s) 62, 212
BstBI TTCGAA 1 cut(s) 493
BstDEI CTNAG 8 cut(s) 350, 404, 470, 591, 654, 694, 960, 975
BstEII GGTNACC 1 cut(s) 725
BstFNI CGCG 1 cut(s) 662
BstKTI GATC 3 cut(s) 295, 349, 432
BstMAI GTCTC 1 cut(s) 394
BstMBI GATC 3 cut(s) 292, 346, 429
BstMWI GCNNNNNNNGC 5 cut(s) 62, 212, 379, 604, 829
BstPI GGTNACC 1 cut(s) 725
BstSFI CTRYAG 1 cut(s) 818
BstSLI GKGCMC 1 cut(s) 834
BstUI CGCG 1 cut(s) 662
BstV1I GCAGC 4 cut(s) 532, 535, 607, 610
BstV2I GAAGAC 1 cut(s) 201
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu36I CCTNAGG 1 cut(s) 654
BtsIMutI CAGTG 2 cut(s) 266, 442
CciI TCATGA 1 cut(s) 295
Cfr10I RCCGGY 1 cut(s) 889
Cfr13I GGNCC 1 cut(s) 1001
CseI GACGC 2 cut(s) 38, 668
Csp6I GTAC 2 cut(s) 324, 850
CviAII CATG 5 cut(s) 139, 296, 583, 615, 1018
CviQI GTAC 2 cut(s) 324, 850
DdeI CTNAG 8 cut(s) 350, 404, 470, 591, 654, 694, 960, 975
DpnI GATC 3 cut(s) 294, 348, 431
DpnII GATC 3 cut(s) 292, 346, 429
Eco130I CCWWGG 1 cut(s) 730
Eco47I GGWCC 1 cut(s) 1001
Eco57I CTGAAG 2 cut(s) 290, 562
Eco81I CCTNAGG 1 cut(s) 654
Eco91I GGTNACC 1 cut(s) 725
EcoO65I GGTNACC 1 cut(s) 725
EcoT14I CCWWGG 1 cut(s) 730
ErhI CCWWGG 1 cut(s) 730
FaeI CATG 5 cut(s) 142, 299, 586, 618, 1021
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 5 cut(s) 138, 295, 582, 614, 1017
FauNDI CATATG 1 cut(s) 456
Fnu4HI GCNGC 4 cut(s) 521, 524, 596, 599
Fsp4HI GCNGC 4 cut(s) 521, 524, 596, 599
FspBI CTAG 4 cut(s) 363, 417, 546, 954
GluI GCNGC 4 cut(s) 521, 524, 596, 599
GsaI CCCAGC 2 cut(s) 382, 827
GsuI CTGGAG 1 cut(s) 587
HapII CCGG 2 cut(s) 153, 890
HgaI GACGC 2 cut(s) 38, 668
Hin1I GRCGYC 1 cut(s) 49
Hin1II CATG 5 cut(s) 142, 299, 586, 618, 1021
HindIII AAGCTT 1 cut(s) 940
HinfI GANTC 6 cut(s) 464, 476, 490, 619, 814, 901
HpaII CCGG 2 cut(s) 153, 890
Hpy188I TCNGA 3 cut(s) 351, 388, 624
Hpy188III TCNNGA 5 cut(s) 283, 296, 749, 765, 908
HpyAV CCTTC 3 cut(s) 143, 171, 586
HpyCH4III ACNGT 2 cut(s) 437, 447
HpyCH4IV ACGT 2 cut(s) 198, 300
HpyCH4V TGCA 4 cut(s) 373, 543, 568, 1010
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 212, 379, 604, 829
HpyF3I CTNAG 8 cut(s) 350, 404, 470, 591, 654, 694, 960, 975
HpySE526I ACGT 2 cut(s) 198, 300
Hsp92I GRCGYC 1 cut(s) 49
Hsp92II CATG 5 cut(s) 142, 299, 586, 618, 1021
Kzo9I GATC 3 cut(s) 292, 346, 429
LmnI GCTCC 2 cut(s) 606, 955
Lsp1109I GCAGC 4 cut(s) 532, 535, 607, 610
MaeI CTAG 4 cut(s) 363, 417, 546, 954
MaeII ACGT 2 cut(s) 198, 300
MaeIII GTNAC 3 cut(s) 353, 725, 1012
MalI GATC 3 cut(s) 294, 348, 431
MboI GATC 3 cut(s) 292, 346, 429
MboII GAAGA 5 cut(s) 85, 206, 283, 473, 836
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 1 cut(s) 834
MluCI AATT 6 cut(s) 101, 248, 314, 508, 878, 982
MlyI GAGTC 1 cut(s) 910
MmeI TCCRAC 5 cut(s) 12, 270, 392, 647, 907
MnlI CCTC 6 cut(s) 21, 269, 337, 649, 715, 915
MroXI GAANNNNTTC 2 cut(s) 160, 278
MseI TTAA 5 cut(s) 24, 303, 483, 794, 933
MspA1I CMGCKG 2 cut(s) 811, 823
MspCI CTTAAG 1 cut(s) 793
MspI CCGG 2 cut(s) 153, 890
Mva1269I GAATGC 2 cut(s) 592, 695
MvnI CGCG 1 cut(s) 662
MwoI GCNNNNNNNGC 5 cut(s) 62, 212, 379, 604, 829
NdeI CATATG 1 cut(s) 456
NdeII GATC 3 cut(s) 292, 346, 429
NlaIII CATG 5 cut(s) 142, 299, 586, 618, 1021
NspV TTCGAA 1 cut(s) 493
PagI TCATGA 1 cut(s) 295
PctI GAATGC 2 cut(s) 592, 695
PdmI GAANNNNTTC 2 cut(s) 160, 278
PfeI GAWTC 5 cut(s) 464, 476, 490, 619, 814
PkrI GCNGC 4 cut(s) 522, 525, 597, 600
PleI GAGTC 1 cut(s) 909
PpsI GAGTC 1 cut(s) 909
PspEI GGTNACC 1 cut(s) 725
PspFI CCCAGC 2 cut(s) 378, 823
PspPI GGNCC 1 cut(s) 1001
PsuI RGATCY 1 cut(s) 346
PvuII CAGCTG 1 cut(s) 823
RsaI GTAC 2 cut(s) 325, 851
RsaNI GTAC 2 cut(s) 324, 850
SaqAI TTAA 5 cut(s) 24, 303, 483, 794, 933
SatI GCNGC 4 cut(s) 521, 524, 596, 599
Sau3AI GATC 3 cut(s) 292, 346, 429
Sau96I GGNCC 1 cut(s) 1001
SchI GAGTC 1 cut(s) 910
SduI GDGCHC 1 cut(s) 834
SfcI CTRYAG 1 cut(s) 818
SfuI TTCGAA 1 cut(s) 493
SinI GGWCC 1 cut(s) 1001
SmlI CTYRAG 2 cut(s) 793, 906
SmoI CTYRAG 2 cut(s) 793, 906
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 6 cut(s) 101, 248, 314, 508, 878, 982
SsiI CCGC 2 cut(s) 804, 809
SspMI CTAG 4 cut(s) 363, 417, 546, 954
StyI CCWWGG 1 cut(s) 730
TaaI ACNGT 2 cut(s) 437, 447
TaiI ACGT 2 cut(s) 201, 303
TaqI TCGA 2 cut(s) 261, 493
TasI AATT 6 cut(s) 101, 248, 314, 508, 878, 982
TfiI GAWTC 5 cut(s) 464, 476, 490, 619, 814
Tru1I TTAA 5 cut(s) 24, 303, 483, 794, 933
Tru9I TTAA 5 cut(s) 24, 303, 483, 794, 933
TscAI CASTG 2 cut(s) 273, 442
TseI GCWGC 4 cut(s) 520, 523, 595, 598
TspDTI ATGAA 4 cut(s) 101, 114, 599, 645
TspGWI ACGGA 1 cut(s) 837
TspRI CASTG 2 cut(s) 273, 442
Vha464I CTTAAG 1 cut(s) 793
VpaK11BI GGWCC 1 cut(s) 1001
XapI RAATTY 3 cut(s) 314, 508, 878
XmnI GAANNNNTTC 2 cut(s) 160, 278
XspI CTAG 4 cut(s) 363, 417, 546, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.