Rroxscaffold_5G00380470
MADS Family

MADS-box transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002890
Physical Location & Seq
Reverse (-)
60622484 .. 60626607
4124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00380470.1

Sequence Viewer

Length: 1056 bp
ATGGGAAGGGTAAAGCTAAAGATAAAGAGGTTGGAGAATACAAATGGGCGTCAAGCAACTTATGCGAAAAGGAAGCATGGCATCATGAAAAAGGCTAATGAATTATCTATACTATGTGACATAGACATTGTCCTTCTTATGTTCTCGCCGACTGGGAAGCCTTCTTTATGCTGCGGAAAACGCAGCATTGAAGAGGTTATAGCAAAGTTTTCCCAACTAACTCCACAGGAAAGAGCTAAAAGGAAACTGGAAAGCCTTGAAGCACTGAAGAAAACATTCAAGAAGTTGGACCATGATGTGAATATACAAGAATTTTTGGGTACGAGTTCTCAAACGGTTGAGGACTTGAGTAACCAATCTAGGTTATTGCAAACCCGACTTTCCGAAATAAAGAAGAGATTAAGCTGTTGGACGAACCCTGATCAGATCAACAATGTAGATCAGTTGGGGCAAATGGAAGATTCAATTAGAGAATCGCTTAACCAGATTCGAACACATAAGGAAAACTTACAAAAACAACAACTAATGTCATTAGAATGCACCAGTCAGTTCCAAAATGGATTGCATATACCCTTCAGCATGAGTACTGAGCAACAACTCCAGCCTCTGCCATGGATTCCTAGTAATGAAAACCGGCATATCGTTTTACCAGAGGACCCGAACTTGCTTCCTCATAGGGATATAGAATGTTCTGCAAGTTCTTCCTTCGGCAGTTACTCTGGTTACCTTGGGACAGGAAAAAGTCCAGAGATTTCCAACTCTGGTCAGGAAAACAGTATTCTAAATGACTTGAGCAGAACTGCGCCACTGAGGCTACAGCTGGGCGGGCAGTTTTCCTCCTACCTTCCATACAATCTCAATATGTTGACCGATACAAAATTTCAACAACCCACAGAAATGAGTCCTCAAGAAAACCCTGTGGAATATCATGTTAATGGAAGCTTTGAAACTCCTAGACCTGATTTTTTCGCTACCCAGCATAGCTGGGCATCTACATCTGGTCCCTGTGCTGTTACTATGTTTGACGAGCATTTATATTCCCAGCAGACAAACTAA

Protein Analysis

351

Amino Acids

39.85

Weight (kDa)

7.11

Isoelectric Point (pI)

59.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 54 4.9e-20 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 174, 825
AcsI RAATTY 2 cut(s) 311, 878
AcuI CTGAAG 2 cut(s) 287, 559
AcyI GRCGYC 1 cut(s) 49
AfaI GTAC 2 cut(s) 322, 586
AgsI TTSAA 6 cut(s) 191, 260, 280, 465, 884, 947
AluBI AGCT 6 cut(s) 16, 236, 405, 820, 942, 984
AluI AGCT 6 cut(s) 16, 236, 405, 820, 942, 984
AlwNI CAGNNNCTG 1 cut(s) 607
ApeKI GCWGC 2 cut(s) 171, 183
ApoI RAATTY 2 cut(s) 311, 878
Asp700I GAANNNNTTC 1 cut(s) 275
AspLEI GCGC 1 cut(s) 805
AspS9I GGNCC 3 cut(s) 289, 655, 1001
AsuII TTCGAA 1 cut(s) 490
AvaII GGWCC 3 cut(s) 289, 655, 1001
BbvI GCAGC 2 cut(s) 158, 195
BclI TGATCA 1 cut(s) 421
BfaI CTAG 3 cut(s) 360, 621, 954
BfmI CTRYAG 1 cut(s) 815
BglI GCCNNNNNGGC 1 cut(s) 811
BisI GCNGC 2 cut(s) 172, 184
BlsI GCNGC 2 cut(s) 173, 185
BmcAI AGTACT 1 cut(s) 586
Bme18I GGWCC 3 cut(s) 289, 655, 1001
BmgT120I GGNCC 3 cut(s) 289, 655, 1001
BmiI GGNNCC 2 cut(s) 657, 1003
BmrI ACTGGG 1 cut(s) 162
BmsI GCATC 2 cut(s) 90, 998
BmuI ACTGGG 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 584
Bpu14I TTCGAA 1 cut(s) 490
BpuEI CTTGAG 3 cut(s) 367, 811, 891
BsaHI GRCGYC 1 cut(s) 49
BsaJI CCNNGG 2 cut(s) 611, 727
Bse118I RCCGGY 1 cut(s) 633
Bse1I ACTGG 3 cut(s) 157, 252, 543
BseDI CCNNGG 2 cut(s) 611, 727
BseMII CTCAG 2 cut(s) 579, 800
BseNI ACTGG 3 cut(s) 157, 252, 543
BseXI GCAGC 2 cut(s) 158, 195
BseYI CCCAGC 4 cut(s) 820, 975, 984, 1041
BsiSI CCGG 1 cut(s) 634
BslFI GGGAC 2 cut(s) 745, 987
BsmFI GGGAC 2 cut(s) 745, 987
BsmI GAATGC 1 cut(s) 542
Bsp119I TTCGAA 1 cut(s) 490
Bsp143I GATC 3 cut(s) 421, 426, 439
Bsp19I CCATGG 1 cut(s) 611
BspACI CCGC 2 cut(s) 174, 825
BspCNI CTCAG 2 cut(s) 580, 801
BspHI TCATGA 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 657, 1003
BspT104I TTCGAA 1 cut(s) 490
BsrFI RCCGGY 1 cut(s) 633
BsrI ACTGG 3 cut(s) 157, 252, 543
BssAI RCCGGY 1 cut(s) 633
BssECI CCNNGG 2 cut(s) 611, 727
BssMI GATC 3 cut(s) 421, 426, 439
BssNI GRCGYC 1 cut(s) 49
BssT1I CCWWGG 2 cut(s) 611, 727
Bst4CI ACNGT 2 cut(s) 337, 776
Bst6I CTCTTC 2 cut(s) 186, 389
BstACI GRCGYC 1 cut(s) 49
BstAPI GCANNNNNTGC 1 cut(s) 62
BstBI TTCGAA 1 cut(s) 490
BstC8I GCNNGC 1 cut(s) 827
BstDEI CTNAG 2 cut(s) 588, 809
BstDSI CCRYGG 1 cut(s) 611
BstEII GGTNACC 1 cut(s) 722
BstHHI GCGC 1 cut(s) 805
BstKTI GATC 3 cut(s) 424, 429, 442
BstMBI GATC 3 cut(s) 421, 426, 439
BstMWI GCNNNNNNNGC 4 cut(s) 62, 180, 811, 826
BstPI GGTNACC 1 cut(s) 722
BstSFI CTRYAG 1 cut(s) 815
BstV1I GCAGC 2 cut(s) 158, 195
BtgI CCRYGG 1 cut(s) 611
BtsIMutI CAGTG 2 cut(s) 263, 806
Cac8I GCNNGC 1 cut(s) 827
CaiI CAGNNNCTG 1 cut(s) 607
CciI TCATGA 1 cut(s) 84
CfoI GCGC 1 cut(s) 805
Cfr10I RCCGGY 1 cut(s) 633
Cfr13I GGNCC 3 cut(s) 289, 655, 1001
CseI GACGC 1 cut(s) 38
Csp6I GTAC 2 cut(s) 321, 585
CviAII CATG 6 cut(s) 77, 85, 293, 580, 612, 929
CviQI GTAC 2 cut(s) 321, 585
DdeI CTNAG 2 cut(s) 588, 809
DpnI GATC 3 cut(s) 423, 428, 441
DpnII GATC 3 cut(s) 421, 426, 439
Eam1104I CTCTTC 2 cut(s) 186, 389
EarI CTCTTC 2 cut(s) 186, 389
Eco130I CCWWGG 2 cut(s) 611, 727
Eco47I GGWCC 3 cut(s) 289, 655, 1001
Eco57I CTGAAG 2 cut(s) 287, 559
Eco91I GGTNACC 1 cut(s) 722
EcoO109I RGGNCCY 1 cut(s) 655
EcoO65I GGTNACC 1 cut(s) 722
EcoT14I CCWWGG 2 cut(s) 611, 727
ErhI CCWWGG 2 cut(s) 611, 727
FaeI CATG 6 cut(s) 80, 88, 296, 583, 615, 932
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 2 cut(s) 745, 987
FatI CATG 6 cut(s) 76, 84, 292, 579, 611, 928
FauI CCCGC 1 cut(s) 818
FbaI TGATCA 1 cut(s) 421
Fnu4HI GCNGC 2 cut(s) 172, 184
Fsp4HI GCNGC 2 cut(s) 172, 184
FspBI CTAG 3 cut(s) 360, 621, 954
GlaI GCGC 1 cut(s) 804
GluI GCNGC 2 cut(s) 172, 184
GsaI CCCAGC 4 cut(s) 824, 979, 988, 1045
GsuI CTGGAG 1 cut(s) 584
HapII CCGG 1 cut(s) 634
HgaI GACGC 1 cut(s) 38
HhaI GCGC 1 cut(s) 805
Hin1I GRCGYC 1 cut(s) 49
Hin1II CATG 6 cut(s) 80, 88, 296, 583, 615, 932
Hin6I GCGC 1 cut(s) 803
HinP1I GCGC 1 cut(s) 803
HincII GTYRAC 1 cut(s) 867
HindII GTYRAC 1 cut(s) 867
HindIII AAGCTT 1 cut(s) 940
HinfI GANTC 5 cut(s) 461, 473, 487, 616, 901
HpaII CCGG 1 cut(s) 634
Hpy166II GTNNAC 1 cut(s) 867
Hpy188I TCNGA 2 cut(s) 385, 426
Hpy188III TCNNGA 5 cut(s) 85, 280, 746, 767, 908
Hpy8I GTNNAC 1 cut(s) 867
HpyAV CCTTC 5 cut(s) 143, 171, 583, 715, 854
HpyCH4III ACNGT 2 cut(s) 337, 776
HpyCH4V TGCA 4 cut(s) 370, 540, 565, 695
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 180, 811, 826
HpyF3I CTNAG 2 cut(s) 588, 809
Hsp92I GRCGYC 1 cut(s) 49
Hsp92II CATG 6 cut(s) 80, 88, 296, 583, 615, 932
HspAI GCGC 1 cut(s) 803
Ksp22I TGATCA 1 cut(s) 421
Kzo9I GATC 3 cut(s) 421, 426, 439
Lsp1109I GCAGC 2 cut(s) 158, 195
LweI GCATC 2 cut(s) 90, 998
MaeI CTAG 3 cut(s) 360, 621, 954
MaeIII GTNAC 5 cut(s) 116, 350, 713, 722, 1012
MalI GATC 3 cut(s) 423, 428, 441
MboI GATC 3 cut(s) 421, 426, 439
MboII GAAGA 5 cut(s) 203, 280, 406, 470, 693
MluCI AATT 4 cut(s) 101, 311, 465, 878
MlyI GAGTC 1 cut(s) 910
MmeI TCCRAC 4 cut(s) 12, 267, 389, 780
MnlI CCTC 9 cut(s) 21, 187, 334, 615, 646, 681, 804, 847, 915
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 3 cut(s) 401, 480, 933
MslI CAYNNNNRTG 3 cut(s) 535, 896, 933
MspA1I CMGCKG 1 cut(s) 820
MspI CCGG 1 cut(s) 634
Mva1269I GAATGC 1 cut(s) 542
MwoI GCNNNNNNNGC 4 cut(s) 62, 180, 811, 826
NcoI CCATGG 1 cut(s) 611
NdeII GATC 3 cut(s) 421, 426, 439
NlaIII CATG 6 cut(s) 80, 88, 296, 583, 615, 932
NlaIV GGNNCC 2 cut(s) 657, 1003
NmuCI GTSAC 1 cut(s) 116
NspV TTCGAA 1 cut(s) 490
PagI TCATGA 1 cut(s) 84
PctI GAATGC 1 cut(s) 542
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 4 cut(s) 461, 473, 487, 616
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 2 cut(s) 173, 185
PleI GAGTC 1 cut(s) 909
PpsI GAGTC 1 cut(s) 909
PpuMI RGGWCCY 1 cut(s) 655
Psp5II RGGWCCY 1 cut(s) 655
PspEI GGTNACC 1 cut(s) 722
PspFI CCCAGC 4 cut(s) 820, 975, 984, 1041
PspN4I GGNNCC 2 cut(s) 657, 1003
PspPI GGNCC 3 cut(s) 289, 655, 1001
PspPPI RGGWCCY 1 cut(s) 655
PstNI CAGNNNCTG 1 cut(s) 607
PsyI GACNNNGTC 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 820
RsaI GTAC 2 cut(s) 322, 586
RsaNI GTAC 2 cut(s) 321, 585
RseI CAYNNNNRTG 3 cut(s) 535, 896, 933
SaqAI TTAA 3 cut(s) 401, 480, 933
SatI GCNGC 2 cut(s) 172, 184
Sau3AI GATC 3 cut(s) 421, 426, 439
Sau96I GGNCC 3 cut(s) 289, 655, 1001
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 1 cut(s) 910
SfaNI GCATC 2 cut(s) 90, 998
SfcI CTRYAG 1 cut(s) 815
SfuI TTCGAA 1 cut(s) 490
SinI GGWCC 3 cut(s) 289, 655, 1001
SmiMI CAYNNNNRTG 3 cut(s) 535, 896, 933
SmlI CTYRAG 3 cut(s) 346, 790, 906
SmoI CTYRAG 3 cut(s) 346, 790, 906
Sse9I AATT 4 cut(s) 101, 311, 465, 878
SsiI CCGC 2 cut(s) 174, 825
SspMI CTAG 3 cut(s) 360, 621, 954
StyI CCWWGG 2 cut(s) 611, 727
TaaI ACNGT 2 cut(s) 337, 776
TaqI TCGA 1 cut(s) 490
TaqII GACCGA 1 cut(s) 884
TasI AATT 4 cut(s) 101, 311, 465, 878
TatI WGTACW 1 cut(s) 584
TfiI GAWTC 4 cut(s) 461, 473, 487, 616
Tru1I TTAA 3 cut(s) 401, 480, 933
Tru9I TTAA 3 cut(s) 401, 480, 933
TscAI CASTG 2 cut(s) 270, 813
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 2 cut(s) 171, 183
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 101, 114, 642
TspRI CASTG 2 cut(s) 270, 813
Tth111I GACNNNGTC 1 cut(s) 128
VpaK11BI GGWCC 3 cut(s) 289, 655, 1001
XapI RAATTY 2 cut(s) 311, 878
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 3 cut(s) 360, 621, 954
ZrmI AGTACT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.