FvH4_4g33340

Vacuolar iron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002581
Physical Location & Seq
Forward (+)
31589081 .. 31594186
5106 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33340.t1

Sequence Viewer

Length: 747 bp
ATGGGGCAAGAAAATGGCTACGGTGACTTGGAGAAGCAGATGCTGGTTCTTCACGACCACGAAGAGAAGCACTTCAGGTCCAGTGAGATTGTCCGTGACGTCATTCTCGGCGTTTCCGACGGTCTCACTGTCCCTTTTGCCCTCGCCGCAGGGTTGTCCGGCGCTGACGTCAGCTCCTCCATTATCCTCGTTGCCGGCATTGCTGAGGTTGCCGCCGGTGCCATCTCCATGGGACTTGGCGGGTACCTTGCGGCCAAGAGTGAAGAAGATCATTACAAGAAAGAACTAAAGCGGGAGCAAGATGAGATCATCGCCGTCCCTGATATAGAGGCTGCTGAGGTTGCAGAAATATTGTCACAGTATGGAGTCGAGGCACATGAATACGGGCCTGTGGTTAACGCCCTAAGAAGGAACCCACAAGCCTGGCTTGATTTCATGATGAAATTTGAGCTAGGACTCGACAAGCCGGACCCAATGAGAGCACTGCAGAGTGCAATGACAATAGCCTTTTCATACGTATGTGGTGGACTGGTGCCTCTCCTTCCCTACATGCTCATTCCAATTGCCACAAAGGCCGTGCTTGCATCTGTGGTTGTCACCATAGTCGCATTGCTGATCTTCGGCTTTGCAAAGGGTCACTTCACCGGAACTCAACCTTTCAAGAGCGCTTTCCAAACCGCCTTGATCGGAGCCATCGCCTCCGCCGCAGCTTACTCCATTGCTAAGCTCTTCCAATCCTACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

249

Amino Acids

26.57

Weight (kDa)

5.12

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 31 - 241 1.1e-73 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 102, 171
Acc65I GGTACC 1 cut(s) 243
AccB1I GGYRCC 3 cut(s) 218, 243, 532
AciI CCGC 8 cut(s) 147, 213, 240, 251, 292, 678, 702, 705
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 58
AcyI GRCGYC 2 cut(s) 99, 168
AfaI GTAC 1 cut(s) 245
AfeI AGCGCT 1 cut(s) 667
AfiI CCNNNNNNNGG 1 cut(s) 408
AgsI TTSAA 1 cut(s) 661
AjnI CCWGG 1 cut(s) 422
AluBI AGCT 4 cut(s) 174, 451, 710, 727
AluI AGCT 4 cut(s) 174, 451, 710, 727
Alw21I GWGCWC 1 cut(s) 484
Alw26I GTCTC 1 cut(s) 128
AlwNI CAGNNNCTG 1 cut(s) 43
Aor51HI AGCGCT 1 cut(s) 667
AoxI GGCC 3 cut(s) 252, 386, 573
ApeKI GCWGC 2 cut(s) 332, 707
ApoI RAATTY 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 71
Asp718I GGTACC 1 cut(s) 243
AspLEI GCGC 2 cut(s) 164, 668
AspS9I GGNCC 3 cut(s) 78, 386, 469
AsuHPI GGTGA 3 cut(s) 35, 589, 634
AvaII GGWCC 2 cut(s) 78, 469
BanI GGYRCC 3 cut(s) 218, 243, 532
Bbv12I GWGCWC 1 cut(s) 484
BbvCI CCTCAGC 2 cut(s) 204, 336
BbvI GCAGC 2 cut(s) 319, 719
BccI CCATC 2 cut(s) 230, 701
BceAI ACGGC 2 cut(s) 299, 560
BciT130I CCWGG 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 1 cut(s) 452
BfmI CTRYAG 1 cut(s) 485
BfoI RGCGCY 2 cut(s) 165, 669
BglI GCCNNNNNGGC 1 cut(s) 572
BisI GCNGC 6 cut(s) 147, 213, 252, 333, 705, 708
BlpI GCTNAGC 1 cut(s) 723
BlsI GCNGC 6 cut(s) 148, 214, 253, 334, 706, 709
Bme1390I CCNGG 1 cut(s) 424
Bme18I GGWCC 2 cut(s) 78, 469
BmgT120I GGNCC 3 cut(s) 78, 386, 469
BmiI GGNNCC 6 cut(s) 220, 245, 413, 471, 534, 691
BmrFI CCNGG 1 cut(s) 424
BmsI GCATC 2 cut(s) 30, 593
Bpu10I CCTNAGC 2 cut(s) 204, 336
Bpu1102I GCTNAGC 1 cut(s) 723
BsaAI YACGTR 1 cut(s) 517
BsaHI GRCGYC 2 cut(s) 99, 168
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 228
BsaWI WCCGGW 1 cut(s) 644
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 1 cut(s) 408
Bse118I RCCGGY 2 cut(s) 194, 215
Bse1I ACTGG 2 cut(s) 81, 534
Bse3DI GCAATG 4 cut(s) 198, 501, 608, 717
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 228
BseLI CCNNNNNNNGG 1 cut(s) 408
BseMI GCAATG 4 cut(s) 198, 501, 608, 717
BseMII CTCAG 2 cut(s) 195, 327
BseNI ACTGG 2 cut(s) 81, 534
BseRI GAGGAG 1 cut(s) 166
BseXI GCAGC 2 cut(s) 319, 719
BshFI GGCC 3 cut(s) 254, 388, 575
BshNI GGYRCC 3 cut(s) 218, 243, 532
BsiHKAI GWGCWC 1 cut(s) 484
BsiSI CCGG 5 cut(s) 159, 195, 216, 467, 645
BslFI GGGAC 3 cut(s) 116, 246, 302
BslI CCNNNNNNNGG 1 cut(s) 408
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 3 cut(s) 116, 246, 302
BsnI GGCC 3 cut(s) 254, 388, 575
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 484
Bsp143I GATC 4 cut(s) 268, 306, 615, 684
Bsp1720I GCTNAGC 1 cut(s) 723
Bsp19I CCATGG 1 cut(s) 228
BspACI CCGC 8 cut(s) 147, 213, 240, 251, 292, 678, 702, 705
BspANI GGCC 3 cut(s) 254, 388, 575
BspCNI CTCAG 2 cut(s) 196, 328
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 6 cut(s) 220, 245, 413, 471, 534, 691
BspMAI CTGCAG 1 cut(s) 489
BspQI GCTCTTC 1 cut(s) 734
BspT107I GGYRCC 3 cut(s) 218, 243, 532
BspTNI GGTCTC 1 cut(s) 128
BsrDI GCAATG 4 cut(s) 198, 501, 608, 717
BsrFI RCCGGY 2 cut(s) 194, 215
BsrI ACTGG 2 cut(s) 81, 534
BssAI RCCGGY 2 cut(s) 194, 215
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 4 cut(s) 268, 306, 615, 684
BssNI GRCGYC 2 cut(s) 99, 168
BssT1I CCWWGG 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 4 cut(s) 23, 122, 130, 360
Bst6I CTCTTC 2 cut(s) 57, 734
BstACI GRCGYC 2 cut(s) 99, 168
BstBAI YACGTR 1 cut(s) 517
BstC8I GCNNGC 2 cut(s) 196, 582
BstDEI CTNAG 4 cut(s) 204, 336, 404, 723
BstDSI CCRYGG 1 cut(s) 228
BstH2I RGCGCY 2 cut(s) 165, 669
BstHHI GCGC 2 cut(s) 164, 668
BstKTI GATC 4 cut(s) 271, 309, 618, 687
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 4 cut(s) 268, 306, 615, 684
BstMWI GCNNNNNNNGC 8 cut(s) 146, 200, 209, 218, 341, 572, 581, 704
BstNI CCWGG 1 cut(s) 424
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 1 cut(s) 422
BstSFI CTRYAG 1 cut(s) 485
BstSNI TACGTA 1 cut(s) 517
BstV1I GCAGC 2 cut(s) 319, 719
BstXI CCANNNNNNTGG 2 cut(s) 229, 423
BsuRI GGCC 3 cut(s) 254, 388, 575
BtgI CCRYGG 1 cut(s) 228
BtgZI GCGATG 2 cut(s) 295, 679
BtsI GCAGTG 1 cut(s) 482
BtsIMutI CAGTG 3 cut(s) 88, 126, 482
Cac8I GCNNGC 2 cut(s) 196, 582
CaiI CAGNNNCTG 1 cut(s) 43
CciI TCATGA 1 cut(s) 435
CfoI GCGC 2 cut(s) 164, 668
Cfr10I RCCGGY 2 cut(s) 194, 215
Cfr13I GGNCC 3 cut(s) 78, 386, 469
Csp6I GTAC 1 cut(s) 244
CviAII CATG 4 cut(s) 229, 377, 436, 550
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 4 cut(s) 204, 336, 404, 723
DpnI GATC 4 cut(s) 270, 308, 617, 686
DpnII GATC 4 cut(s) 268, 306, 615, 684
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 2 cut(s) 57, 734
EarI CTCTTC 2 cut(s) 57, 734
EciI GGCGGA 1 cut(s) 691
Eco105I TACGTA 1 cut(s) 517
Eco130I CCWWGG 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 128
Eco47I GGWCC 2 cut(s) 78, 469
Eco47III AGCGCT 1 cut(s) 667
Eco57I CTGAAG 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 422
EcoT14I CCWWGG 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 4 cut(s) 232, 380, 439, 553
FaiI YATR 9 cut(s) 230, 326, 363, 378, 437, 514, 520, 551, 602
FalI AAGNNNNNCTT 4 cut(s) 411, 443, 623, 655
FaqI GGGAC 3 cut(s) 116, 246, 302
FatI CATG 4 cut(s) 228, 376, 435, 549
FauI CCCGC 2 cut(s) 233, 285
Fnu4HI GCNGC 6 cut(s) 147, 213, 252, 333, 705, 708
Fsp4HI GCNGC 6 cut(s) 147, 213, 252, 333, 705, 708
FspBI CTAG 1 cut(s) 452
GlaI GCGC 2 cut(s) 163, 667
GluI GCNGC 6 cut(s) 147, 213, 252, 333, 705, 708
HaeII RGCGCY 2 cut(s) 165, 669
HaeIII GGCC 3 cut(s) 254, 388, 575
HapII CCGG 5 cut(s) 159, 195, 216, 467, 645
HhaI GCGC 2 cut(s) 164, 668
Hin1I GRCGYC 2 cut(s) 99, 168
Hin1II CATG 4 cut(s) 232, 380, 439, 553
Hin6I GCGC 2 cut(s) 162, 666
HinP1I GCGC 2 cut(s) 162, 666
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 2 cut(s) 366, 456
HpaI GTTAAC 1 cut(s) 397
HpaII CCGG 5 cut(s) 159, 195, 216, 467, 645
HphI GGTGA 3 cut(s) 35, 589, 634
Hpy166II GTNNAC 2 cut(s) 397, 527
Hpy188I TCNGA 2 cut(s) 118, 689
Hpy188III TCNNGA 3 cut(s) 53, 436, 661
Hpy8I GTNNAC 2 cut(s) 397, 527
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 2 cut(s) 402, 551
HpyCH4III ACNGT 4 cut(s) 23, 122, 130, 360
HpyCH4IV ACGT 3 cut(s) 99, 168, 516
HpyCH4V TGCA 5 cut(s) 344, 487, 494, 584, 629
HpyF10VI GCNNNNNNNGC 8 cut(s) 146, 200, 209, 218, 341, 572, 581, 704
HpyF3I CTNAG 4 cut(s) 204, 336, 404, 723
HpySE526I ACGT 3 cut(s) 99, 168, 516
Hsp92I GRCGYC 2 cut(s) 99, 168
Hsp92II CATG 4 cut(s) 232, 380, 439, 553
HspAI GCGC 2 cut(s) 162, 666
KpnI GGTACC 1 cut(s) 247
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
KspAI GTTAAC 1 cut(s) 397
Kzo9I GATC 4 cut(s) 268, 306, 615, 684
LguI GCTCTTC 1 cut(s) 734
LmnI GCTCC 3 cut(s) 179, 295, 689
Lsp1109I GCAGC 2 cut(s) 319, 719
LweI GCATC 2 cut(s) 30, 593
MaeI CTAG 1 cut(s) 452
MaeII ACGT 3 cut(s) 99, 168, 516
MaeIII GTNAC 5 cut(s) 23, 95, 354, 595, 635
MalI GATC 4 cut(s) 270, 308, 617, 686
MboI GATC 4 cut(s) 268, 306, 615, 684
MboII GAAGA 6 cut(s) 41, 74, 275, 278, 610, 721
MfeI CAATTG 1 cut(s) 561
MhlI GDGCHC 1 cut(s) 484
MluCI AATT 2 cut(s) 443, 561
MlyI GAGTC 2 cut(s) 375, 450
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 9 cut(s) 152, 187, 197, 199, 322, 331, 364, 546, 709
MroNI GCCGGC 1 cut(s) 194
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 1 cut(s) 396
MslI CAYNNNNRTG 2 cut(s) 227, 517
MspI CCGG 5 cut(s) 159, 195, 216, 467, 645
MspR9I CCNGG 1 cut(s) 424
MunI CAATTG 1 cut(s) 561
MvaI CCWGG 1 cut(s) 424
MwoI GCNNNNNNNGC 8 cut(s) 146, 200, 209, 218, 341, 572, 581, 704
NaeI GCCGGC 1 cut(s) 196
NcoI CCATGG 1 cut(s) 228
NdeII GATC 4 cut(s) 268, 306, 615, 684
NgoMIV GCCGGC 1 cut(s) 194
NlaIII CATG 4 cut(s) 232, 380, 439, 553
NlaIV GGNNCC 6 cut(s) 220, 245, 413, 471, 534, 691
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 5 cut(s) 23, 95, 354, 595, 635
NspI RCATGY 1 cut(s) 553
PagI TCATGA 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 734
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PdiI GCCGGC 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 71
PkrI GCNGC 6 cut(s) 148, 214, 253, 334, 706, 709
PleI GAGTC 2 cut(s) 374, 450
PpsI GAGTC 2 cut(s) 374, 450
Ppu21I YACGTR 1 cut(s) 517
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspN4I GGNNCC 6 cut(s) 220, 245, 413, 471, 534, 691
PspPI GGNCC 3 cut(s) 78, 386, 469
PstI CTGCAG 1 cut(s) 489
PstNI CAGNNNCTG 1 cut(s) 43
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 2 cut(s) 227, 517
SapI GCTCTTC 1 cut(s) 734
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 6 cut(s) 147, 213, 252, 333, 705, 708
Sau3AI GATC 4 cut(s) 268, 306, 615, 684
Sau96I GGNCC 3 cut(s) 78, 386, 469
SchI GAGTC 2 cut(s) 375, 450
ScrFI CCNGG 1 cut(s) 424
SduI GDGCHC 1 cut(s) 484
SfaNI GCATC 2 cut(s) 30, 593
SfcI CTRYAG 1 cut(s) 485
SgrAI CRCCGGYG 1 cut(s) 215
SinI GGWCC 2 cut(s) 78, 469
SmiMI CAYNNNNRTG 2 cut(s) 227, 517
SnaBI TACGTA 1 cut(s) 517
Sse9I AATT 2 cut(s) 443, 561
SsiI CCGC 8 cut(s) 147, 213, 240, 251, 292, 678, 702, 705
SspI AATATT 1 cut(s) 351
SspMI CTAG 1 cut(s) 452
StyD4I CCNGG 1 cut(s) 422
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 4 cut(s) 23, 122, 130, 360
TaiI ACGT 3 cut(s) 102, 171, 519
TaqI TCGA 2 cut(s) 369, 459
TasI AATT 2 cut(s) 443, 561
TauI GCSGC 4 cut(s) 149, 215, 254, 707
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 3 cut(s) 88, 133, 489
TseFI GTSAC 5 cut(s) 23, 95, 354, 595, 635
TseI GCWGC 2 cut(s) 332, 707
Tsp45I GTSAC 5 cut(s) 23, 95, 354, 595, 635
TspDTI ATGAA 4 cut(s) 393, 424, 455, 501
TspGWI ACGGA 1 cut(s) 83
TspRI CASTG 3 cut(s) 88, 133, 489
VpaK11BI GGWCC 2 cut(s) 78, 469
XapI RAATTY 1 cut(s) 443
XceI RCATGY 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 1 cut(s) 452
ZraI GACGTC 2 cut(s) 100, 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.