Rw4G034030

Vacuolar iron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002581
Physical Location & Seq
Forward (+)
61149784 .. 61151347
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034030.1

Sequence Viewer

Length: 753 bp
ATGGGGAGTGATCTTGACAATGGCTATGGTGACTTGGAGAAGCAGATGCTGGTTCTTCACGAACACGAAGAGAAGCACTTCATGTCCAGTGAGATTGTCCGTGACGTCATTCTCGGCGTCTCCGACGGTCTCACTGTCCCTTTTGCCCTCGCCGCGGGGTTGTCCGGCGCTGATGTCAGCTCCTCCATTATCCTCATTGCCGGCATTGCTGAGGTTGCCGCCGGTGCCATCTCCATGGGACTTGGCGGGTATCTTGCGGCGAAGAGTGAAGAAGATCATTACAAGAAAGAATTAAAGAGGGAGCAAGATGAAATCATCGCCGTCCCTGATATAGAAGCTGCTGAGGTTGCAGAAATCTTGTCACAATATGGGGTTGAAGCACGTGAATATGGGCCTGTGGTGAATGCTCTAAGAAGGAACCCTCAAGCTTGGCTTGATTTCATGATGAAATTTGAACTAGGACTCGACAAGCCAGACCCAATGAGAGCGCTGCAGAGTGCAATGACAATAGCCTTTTCATACGTCTGTGGTGGACTGGTGCCTCTCCTTCCCTACATGCTCATTCCAGTTGCTACAAAGGCGGCGCTTGCATCTGTGGTGGTCACCATAGTGGCATTGCTGATCTTCGGCTTTGCAAAGGGTCACTTCACCGGAACTCAACCTTTCAAGAGCGCTTTCCAAACCGCCTTCATTGGAGCCATCGCATCCGCCGCAGCTTATTCCATTGCGAAACTCTTCCAATCCTACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

250

Amino Acids

26.76

Weight (kDa)

4.94

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 33 - 243 2.2e-73 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 108
AccB1I GGYRCC 2 cut(s) 224, 538
AccII CGCG 1 cut(s) 155
AciI CCGC 9 cut(s) 153, 155, 219, 246, 257, 581, 684, 708, 711
AcsI RAATTY 1 cut(s) 449
AcvI CACGTG 1 cut(s) 383
AcyI GRCGYC 2 cut(s) 105, 117
AfeI AGCGCT 2 cut(s) 489, 673
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 3 cut(s) 377, 455, 667
AleI CACNNNNGTG 1 cut(s) 608
AluBI AGCT 4 cut(s) 180, 338, 428, 716
AluI AGCT 4 cut(s) 180, 338, 428, 716
Alw26I GTCTC 2 cut(s) 124, 134
AlwNI CAGNNNCTG 1 cut(s) 49
Aor51HI AGCGCT 2 cut(s) 489, 673
AoxI GGCC 1 cut(s) 392
ApeKI GCWGC 3 cut(s) 338, 490, 713
ApoI RAATTY 1 cut(s) 449
Asp700I GAANNNNTTC 2 cut(s) 77, 734
AspLEI GCGC 4 cut(s) 170, 490, 586, 674
AspS9I GGNCC 1 cut(s) 392
AsuHPI GGTGA 4 cut(s) 41, 412, 595, 640
BanI GGYRCC 2 cut(s) 224, 538
BbrPI CACGTG 1 cut(s) 383
BbvCI CCTCAGC 2 cut(s) 210, 342
BbvI GCAGC 3 cut(s) 325, 477, 725
BccI CCATC 2 cut(s) 236, 707
BceAI ACGGC 1 cut(s) 305
BcoDI GTCTC 2 cut(s) 124, 134
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 491
BfoI RGCGCY 4 cut(s) 171, 491, 587, 675
BisI GCNGC 8 cut(s) 153, 219, 258, 339, 491, 582, 711, 714
BlsI GCNGC 8 cut(s) 154, 220, 259, 340, 492, 583, 712, 715
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 4 cut(s) 226, 419, 540, 697
BmsI GCATC 3 cut(s) 36, 599, 713
Bpu10I CCTNAGC 2 cut(s) 210, 342
BpuEI CTTGAG 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 383
BsaHI GRCGYC 2 cut(s) 105, 117
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 153, 234
BsaWI WCCGGW 1 cut(s) 650
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse118I RCCGGY 2 cut(s) 200, 221
Bse1I ACTGG 3 cut(s) 87, 540, 566
Bse3DI GCAATG 5 cut(s) 195, 204, 507, 614, 723
BseDI CCNNGG 2 cut(s) 153, 234
BseGI GGATG 1 cut(s) 704
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMI GCAATG 5 cut(s) 195, 204, 507, 614, 723
BseMII CTCAG 2 cut(s) 201, 333
BseNI ACTGG 3 cut(s) 87, 540, 566
BseRI GAGGAG 1 cut(s) 172
BseXI GCAGC 3 cut(s) 325, 477, 725
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 1 cut(s) 394
BshNI GGYRCC 2 cut(s) 224, 538
BsiSI CCGG 4 cut(s) 165, 201, 222, 651
BslFI GGGAC 3 cut(s) 122, 252, 308
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 2 cut(s) 124, 134
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 3 cut(s) 122, 252, 308
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 1 cut(s) 394
Bso31I GGTCTC 1 cut(s) 134
Bsp143I GATC 3 cut(s) 10, 274, 621
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 9 cut(s) 153, 155, 219, 246, 257, 581, 684, 708, 711
BspANI GGCC 1 cut(s) 394
BspCNI CTCAG 2 cut(s) 202, 334
BspFNI CGCG 1 cut(s) 155
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 4 cut(s) 226, 419, 540, 697
BspMAI CTGCAG 1 cut(s) 495
BspT107I GGYRCC 2 cut(s) 224, 538
BspTNI GGTCTC 1 cut(s) 134
BsrDI GCAATG 5 cut(s) 195, 204, 507, 614, 723
BsrFI RCCGGY 2 cut(s) 200, 221
BsrI ACTGG 3 cut(s) 87, 540, 566
BssAI RCCGGY 2 cut(s) 200, 221
BssECI CCNNGG 2 cut(s) 153, 234
BssMI GATC 3 cut(s) 10, 274, 621
BssNI GRCGYC 2 cut(s) 105, 117
BssT1I CCWWGG 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 128, 136
Bst6I CTCTTC 3 cut(s) 63, 257, 740
BstACI GRCGYC 2 cut(s) 105, 117
BstBAI YACGTR 1 cut(s) 383
BstC8I GCNNGC 2 cut(s) 202, 588
BstDEI CTNAG 3 cut(s) 210, 342, 410
BstDSI CCRYGG 2 cut(s) 153, 234
BstEII GGTNACC 1 cut(s) 601
BstF5I GGATG 1 cut(s) 704
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 4 cut(s) 171, 491, 587, 675
BstHHI GCGC 4 cut(s) 170, 490, 586, 674
BstKTI GATC 3 cut(s) 13, 277, 624
BstMAI GTCTC 2 cut(s) 124, 134
BstMBI GATC 3 cut(s) 10, 274, 621
BstMWI GCNNNNNNNGC 8 cut(s) 152, 206, 215, 224, 347, 578, 587, 710
BstNSI RCATGY 1 cut(s) 559
BstPI GGTNACC 1 cut(s) 601
BstSFI CTRYAG 1 cut(s) 491
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 3 cut(s) 325, 477, 725
BstXI CCANNNNNNTGG 1 cut(s) 235
BsuRI GGCC 1 cut(s) 394
BtgI CCRYGG 2 cut(s) 153, 234
BtgZI GCGATG 2 cut(s) 301, 685
BtsCI GGATG 1 cut(s) 704
BtsIMutI CAGTG 2 cut(s) 94, 132
Cac8I GCNNGC 2 cut(s) 202, 588
CaiI CAGNNNCTG 1 cut(s) 49
CciI TCATGA 1 cut(s) 441
CfoI GCGC 4 cut(s) 170, 490, 586, 674
Cfr10I RCCGGY 2 cut(s) 200, 221
Cfr13I GGNCC 1 cut(s) 392
Cfr42I CCGCGG 1 cut(s) 156
CseI GACGC 1 cut(s) 106
CviAII CATG 4 cut(s) 82, 235, 442, 556
DdeI CTNAG 3 cut(s) 210, 342, 410
DpnI GATC 3 cut(s) 12, 276, 623
DpnII GATC 3 cut(s) 10, 274, 621
Eam1104I CTCTTC 3 cut(s) 63, 257, 740
EarI CTCTTC 3 cut(s) 63, 257, 740
EciI GGCGGA 1 cut(s) 697
Eco130I CCWWGG 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 134
Eco47III AGCGCT 2 cut(s) 489, 673
Eco72I CACGTG 1 cut(s) 383
Eco91I GGTNACC 1 cut(s) 601
EcoO65I GGTNACC 1 cut(s) 601
EcoT14I CCWWGG 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 4 cut(s) 85, 238, 445, 559
FalI AAGNNNNNCTT 4 cut(s) 417, 449, 629, 661
FaqI GGGAC 3 cut(s) 122, 252, 308
FatI CATG 4 cut(s) 81, 234, 441, 555
FauI CCCGC 2 cut(s) 148, 239
Fnu4HI GCNGC 8 cut(s) 153, 219, 258, 339, 491, 582, 711, 714
FokI GGATG 1 cut(s) 691
Fsp4HI GCNGC 8 cut(s) 153, 219, 258, 339, 491, 582, 711, 714
FspBI CTAG 1 cut(s) 458
GlaI GCGC 4 cut(s) 169, 489, 585, 673
GluI GCNGC 8 cut(s) 153, 219, 258, 339, 491, 582, 711, 714
HaeII RGCGCY 4 cut(s) 171, 491, 587, 675
HaeIII GGCC 1 cut(s) 394
HapII CCGG 4 cut(s) 165, 201, 222, 651
HgaI GACGC 1 cut(s) 106
HhaI GCGC 4 cut(s) 170, 490, 586, 674
Hin1I GRCGYC 2 cut(s) 105, 117
Hin1II CATG 4 cut(s) 85, 238, 445, 559
Hin6I GCGC 4 cut(s) 168, 488, 584, 672
HinP1I GCGC 4 cut(s) 168, 488, 584, 672
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 1 cut(s) 462
HpaII CCGG 4 cut(s) 165, 201, 222, 651
HphI GGTGA 4 cut(s) 41, 412, 595, 640
Hpy166II GTNNAC 1 cut(s) 533
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 4 cut(s) 14, 59, 442, 667
Hpy8I GTNNAC 1 cut(s) 533
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 408, 557, 697
HpyCH4III ACNGT 2 cut(s) 128, 136
HpyCH4IV ACGT 3 cut(s) 105, 382, 522
HpyCH4V TGCA 5 cut(s) 350, 493, 500, 590, 635
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 206, 215, 224, 347, 578, 587, 710
HpyF3I CTNAG 3 cut(s) 210, 342, 410
HpySE526I ACGT 3 cut(s) 105, 382, 522
Hsp92I GRCGYC 2 cut(s) 105, 117
Hsp92II CATG 4 cut(s) 85, 238, 445, 559
HspAI GCGC 4 cut(s) 168, 488, 584, 672
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 3 cut(s) 10, 274, 621
LmnI GCTCC 3 cut(s) 185, 301, 695
Lsp1109I GCAGC 3 cut(s) 325, 477, 725
LweI GCATC 3 cut(s) 36, 599, 713
MaeI CTAG 1 cut(s) 458
MaeII ACGT 3 cut(s) 105, 382, 522
MaeIII GTNAC 5 cut(s) 29, 101, 360, 601, 641
MalI GATC 3 cut(s) 12, 276, 623
MboI GATC 3 cut(s) 10, 274, 621
MboII GAAGA 7 cut(s) 47, 80, 274, 281, 284, 616, 727
MluCI AATT 2 cut(s) 290, 449
MlyI GAGTC 1 cut(s) 456
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 8 cut(s) 158, 193, 203, 205, 291, 337, 432, 552
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 2 cut(s) 77, 734
MseI TTAA 1 cut(s) 293
MslI CAYNNNNRTG 2 cut(s) 233, 608
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 4 cut(s) 165, 201, 222, 651
Mva1269I GAATGC 1 cut(s) 409
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 8 cut(s) 152, 206, 215, 224, 347, 578, 587, 710
NaeI GCCGGC 1 cut(s) 202
NcoI CCATGG 1 cut(s) 234
NdeII GATC 3 cut(s) 10, 274, 621
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 4 cut(s) 85, 238, 445, 559
NlaIV GGNNCC 4 cut(s) 226, 419, 540, 697
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 5 cut(s) 29, 101, 360, 601, 641
NspI RCATGY 1 cut(s) 559
OliI CACNNNNGTG 1 cut(s) 608
PagI TCATGA 1 cut(s) 441
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 1 cut(s) 409
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 2 cut(s) 77, 734
PkrI GCNGC 8 cut(s) 154, 220, 259, 340, 492, 583, 712, 715
PleI GAGTC 1 cut(s) 456
PmaCI CACGTG 1 cut(s) 383
PmlI CACGTG 1 cut(s) 383
PpsI GAGTC 1 cut(s) 456
Ppu21I YACGTR 1 cut(s) 383
PspCI CACGTG 1 cut(s) 383
PspEI GGTNACC 1 cut(s) 601
PspN4I GGNNCC 4 cut(s) 226, 419, 540, 697
PspPI GGNCC 1 cut(s) 392
PstI CTGCAG 1 cut(s) 495
PstNI CAGNNNCTG 1 cut(s) 49
RseI CAYNNNNRTG 2 cut(s) 233, 608
SacII CCGCGG 1 cut(s) 156
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 8 cut(s) 153, 219, 258, 339, 491, 582, 711, 714
Sau3AI GATC 3 cut(s) 10, 274, 621
Sau96I GGNCC 1 cut(s) 392
SchI GAGTC 1 cut(s) 456
SfaNI GCATC 3 cut(s) 36, 599, 713
SfcI CTRYAG 1 cut(s) 491
Sfr303I CCGCGG 1 cut(s) 156
SgrAI CRCCGGYG 1 cut(s) 221
SgrBI CCGCGG 1 cut(s) 156
SmiMI CAYNNNNRTG 2 cut(s) 233, 608
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
Sse9I AATT 2 cut(s) 290, 449
SsiI CCGC 9 cut(s) 153, 155, 219, 246, 257, 581, 684, 708, 711
SspMI CTAG 1 cut(s) 458
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 2 cut(s) 128, 136
TaiI ACGT 3 cut(s) 108, 385, 525
TaqI TCGA 1 cut(s) 465
TasI AATT 2 cut(s) 290, 449
TauI GCSGC 5 cut(s) 155, 221, 260, 584, 713
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TscAI CASTG 2 cut(s) 94, 139
TseFI GTSAC 5 cut(s) 29, 101, 360, 601, 641
TseI GCWGC 3 cut(s) 338, 490, 713
Tsp45I GTSAC 5 cut(s) 29, 101, 360, 601, 641
TspDTI ATGAA 6 cut(s) 70, 324, 430, 461, 507, 679
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 2 cut(s) 94, 139
XapI RAATTY 1 cut(s) 449
XceI RCATGY 1 cut(s) 559
XmnI GAANNNNTTC 2 cut(s) 77, 734
XspI CTAG 1 cut(s) 458
ZraI GACGTC 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.