Rroxscaffold_5G00383390

Vacuolar iron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002581
Physical Location & Seq
Forward (+)
63003708 .. 63005224
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383390.1

Sequence Viewer

Length: 510 bp
ATGTTGTATCTTGTGGCGAAGAGTGAAGAAGATCATTACAAGAAAGAACTAAAGAGGGAGCAAGATGAAATCATCGCCGTCCCTGATATAGAAGCTGCCGAGGTTGCAGAAATCTTGTCACAATATGGGGTTGAAGCACGTGAATATGGGCCTGTGGTGAATGCTCTAAGAAGGAACCCTCAAGCTTGGCTTGATTTCATGATGAAATTTGAACTAGGACTCGACAAGCCGGACCCAATGAGAGCGCTGCAGAGTGCAATGACAATAGCCTTTTCATACGTCTGTGGTGGACTGGTGCCTCTCCTTCCCTACATGCTCATTCCAGTTGCTACAAAGGCGGTGCTTGCATCTGTGGTGGTCACCATAGTGGCATTGCTGATCTTCGGCTTTGCAAAGGGTCACTTCACCGGAACTCAACCTTTCAAGAGCGCTTTCCAAACCGCCTTCATTGGAGCCATCGCATCCGCCGCAGCTTATTCCATTGCTAAACTCTTCCAATCCTACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

169

Amino Acids

18.62

Weight (kDa)

5.91

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 3 - 162 2.3e-47 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 295
AciI CCGC 4 cut(s) 338, 441, 465, 468
AcsI RAATTY 1 cut(s) 206
AcvI CACGTG 1 cut(s) 140
AfeI AGCGCT 2 cut(s) 246, 430
AgsI TTSAA 3 cut(s) 134, 212, 424
AleI CACNNNNGTG 1 cut(s) 365
AluBI AGCT 3 cut(s) 95, 185, 473
AluI AGCT 3 cut(s) 95, 185, 473
Aor51HI AGCGCT 2 cut(s) 246, 430
AoxI GGCC 1 cut(s) 149
ApeKI GCWGC 3 cut(s) 95, 247, 470
ApoI RAATTY 1 cut(s) 206
AspLEI GCGC 2 cut(s) 247, 431
AspS9I GGNCC 2 cut(s) 149, 232
AsuHPI GGTGA 3 cut(s) 169, 352, 397
AvaII GGWCC 1 cut(s) 232
BanI GGYRCC 1 cut(s) 295
BbrPI CACGTG 1 cut(s) 140
BbvI GCAGC 3 cut(s) 82, 234, 482
BccI CCATC 1 cut(s) 464
BceAI ACGGC 1 cut(s) 62
BfaI CTAG 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 248
BfoI RGCGCY 2 cut(s) 248, 432
BisI GCNGC 4 cut(s) 96, 248, 468, 471
BlsI GCNGC 4 cut(s) 97, 249, 469, 472
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 2 cut(s) 149, 232
BmiI GGNNCC 4 cut(s) 176, 234, 297, 454
BmsI GCATC 2 cut(s) 356, 470
BpuEI CTTGAG 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 99
BsaWI WCCGGW 1 cut(s) 407
Bse1I ACTGG 2 cut(s) 297, 323
Bse3DI GCAATG 3 cut(s) 264, 371, 480
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 1 cut(s) 461
BseMI GCAATG 3 cut(s) 264, 371, 480
BseNI ACTGG 2 cut(s) 297, 323
BseXI GCAGC 3 cut(s) 82, 234, 482
BshFI GGCC 1 cut(s) 151
BshNI GGYRCC 1 cut(s) 295
BsiSI CCGG 2 cut(s) 230, 408
BslFI GGGAC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 65
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 1 cut(s) 151
Bsp143I GATC 2 cut(s) 31, 378
BspACI CCGC 4 cut(s) 338, 441, 465, 468
BspANI GGCC 1 cut(s) 151
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 4 cut(s) 176, 234, 297, 454
BspMAI CTGCAG 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 295
BsrDI GCAATG 3 cut(s) 264, 371, 480
BsrI ACTGG 2 cut(s) 297, 323
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 2 cut(s) 31, 378
Bst6I CTCTTC 2 cut(s) 14, 497
BstBAI YACGTR 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 1 cut(s) 167
BstEII GGTNACC 1 cut(s) 358
BstF5I GGATG 1 cut(s) 461
BstH2I RGCGCY 2 cut(s) 248, 432
BstHHI GCGC 2 cut(s) 247, 431
BstKTI GATC 2 cut(s) 34, 381
BstMBI GATC 2 cut(s) 31, 378
BstMWI GCNNNNNNNGC 4 cut(s) 104, 335, 344, 467
BstNSI RCATGY 1 cut(s) 316
BstPI GGTNACC 1 cut(s) 358
BstSFI CTRYAG 1 cut(s) 248
BstV1I GCAGC 3 cut(s) 82, 234, 482
BsuRI GGCC 1 cut(s) 151
BtgZI GCGATG 2 cut(s) 58, 442
BtsCI GGATG 1 cut(s) 461
Cac8I GCNNGC 1 cut(s) 345
CciI TCATGA 1 cut(s) 198
CfoI GCGC 2 cut(s) 247, 431
Cfr13I GGNCC 2 cut(s) 149, 232
CviAII CATG 2 cut(s) 199, 313
CviJI RGCY 9 cut(s) 95, 151, 185, 190, 229, 269, 387, 455, 473
CviKI_1 RGCY 9 cut(s) 95, 151, 185, 190, 229, 269, 387, 455, 473
DdeI CTNAG 1 cut(s) 167
DpnI GATC 2 cut(s) 33, 380
DpnII GATC 2 cut(s) 31, 378
Eam1104I CTCTTC 2 cut(s) 14, 497
EarI CTCTTC 2 cut(s) 14, 497
EciI GGCGGA 1 cut(s) 454
Eco47I GGWCC 1 cut(s) 232
Eco47III AGCGCT 2 cut(s) 246, 430
Eco72I CACGTG 1 cut(s) 140
Eco91I GGTNACC 1 cut(s) 358
EcoO65I GGTNACC 1 cut(s) 358
FaeI CATG 2 cut(s) 202, 316
FaiI YATR 7 cut(s) 89, 126, 147, 200, 277, 314, 365
FalI AAGNNNNNCTT 4 cut(s) 174, 206, 386, 418
FaqI GGGAC 1 cut(s) 65
FatI CATG 2 cut(s) 198, 312
Fnu4HI GCNGC 4 cut(s) 96, 248, 468, 471
FokI GGATG 1 cut(s) 448
Fsp4HI GCNGC 4 cut(s) 96, 248, 468, 471
FspBI CTAG 1 cut(s) 215
GlaI GCGC 2 cut(s) 246, 430
GluI GCNGC 4 cut(s) 96, 248, 468, 471
HaeII RGCGCY 2 cut(s) 248, 432
HaeIII GGCC 1 cut(s) 151
HapII CCGG 2 cut(s) 230, 408
HhaI GCGC 2 cut(s) 247, 431
Hin1II CATG 2 cut(s) 202, 316
Hin6I GCGC 2 cut(s) 245, 429
HinP1I GCGC 2 cut(s) 245, 429
HindIII AAGCTT 1 cut(s) 183
HinfI GANTC 1 cut(s) 219
HpaII CCGG 2 cut(s) 230, 408
HphI GGTGA 3 cut(s) 169, 352, 397
Hpy166II GTNNAC 1 cut(s) 290
Hpy188III TCNNGA 2 cut(s) 199, 424
Hpy8I GTNNAC 1 cut(s) 290
HpyAV CCTTC 3 cut(s) 165, 314, 454
HpyCH4IV ACGT 2 cut(s) 139, 279
HpyCH4V TGCA 5 cut(s) 107, 250, 257, 347, 392
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 335, 344, 467
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 2 cut(s) 139, 279
Hsp92II CATG 2 cut(s) 202, 316
HspAI GCGC 2 cut(s) 245, 429
Kzo9I GATC 2 cut(s) 31, 378
LmnI GCTCC 2 cut(s) 58, 452
LpnPI CCDG 6 cut(s) 96, 165, 243, 278, 336, 421
Lsp1109I GCAGC 3 cut(s) 82, 234, 482
LweI GCATC 2 cut(s) 356, 470
MaeI CTAG 1 cut(s) 215
MaeII ACGT 2 cut(s) 139, 279
MaeIII GTNAC 3 cut(s) 117, 358, 398
MalI GATC 2 cut(s) 33, 380
MboI GATC 2 cut(s) 31, 378
MboII GAAGA 5 cut(s) 31, 38, 41, 373, 484
MluCI AATT 1 cut(s) 206
MlyI GAGTC 1 cut(s) 213
MnlI CCTC 4 cut(s) 48, 94, 189, 309
MslI CAYNNNNRTG 1 cut(s) 365
MspI CCGG 2 cut(s) 230, 408
Mva1269I GAATGC 1 cut(s) 166
MwoI GCNNNNNNNGC 4 cut(s) 104, 335, 344, 467
NdeII GATC 2 cut(s) 31, 378
NlaIII CATG 2 cut(s) 202, 316
NlaIV GGNNCC 4 cut(s) 176, 234, 297, 454
NmeAIII GCCGAG 1 cut(s) 124
NmuCI GTSAC 3 cut(s) 117, 358, 398
NspI RCATGY 1 cut(s) 316
OliI CACNNNNGTG 1 cut(s) 365
PagI TCATGA 1 cut(s) 198
PctI GAATGC 1 cut(s) 166
PkrI GCNGC 4 cut(s) 97, 249, 469, 472
PleI GAGTC 1 cut(s) 213
PmaCI CACGTG 1 cut(s) 140
PmlI CACGTG 1 cut(s) 140
PpsI GAGTC 1 cut(s) 213
Ppu21I YACGTR 1 cut(s) 140
PspCI CACGTG 1 cut(s) 140
PspEI GGTNACC 1 cut(s) 358
PspN4I GGNNCC 4 cut(s) 176, 234, 297, 454
PspPI GGNCC 2 cut(s) 149, 232
PstI CTGCAG 1 cut(s) 252
RseI CAYNNNNRTG 1 cut(s) 365
SatI GCNGC 4 cut(s) 96, 248, 468, 471
Sau3AI GATC 2 cut(s) 31, 378
Sau96I GGNCC 2 cut(s) 149, 232
SchI GAGTC 1 cut(s) 213
SetI ASST 7 cut(s) 97, 105, 142, 187, 282, 421, 475
SfaNI GCATC 2 cut(s) 356, 470
SfcI CTRYAG 1 cut(s) 248
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 365
SmlI CTYRAG 1 cut(s) 180
SmoI CTYRAG 1 cut(s) 180
Sse9I AATT 1 cut(s) 206
SsiI CCGC 4 cut(s) 338, 441, 465, 468
SspMI CTAG 1 cut(s) 215
TaiI ACGT 2 cut(s) 142, 282
TaqI TCGA 1 cut(s) 222
TasI AATT 1 cut(s) 206
TauI GCSGC 1 cut(s) 470
TseFI GTSAC 3 cut(s) 117, 358, 398
TseI GCWGC 3 cut(s) 95, 247, 470
Tsp45I GTSAC 3 cut(s) 117, 358, 398
TspDTI ATGAA 5 cut(s) 81, 187, 218, 264, 436
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 1 cut(s) 206
XceI RCATGY 1 cut(s) 316
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.