Rh4BG406900

Vacuolar iron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002581
Physical Location & Seq
Forward (+)
55682490 .. 55684067
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG406900.1

Sequence Viewer

Length: 627 bp
ATGGGGAGTGATCATGACAATGGCTATGGTGACTTGGAGAAGCAGATGCTGGTTCTTCACGAACACGAAGAGAAGCACTTCATGTCCAGTGAGATTGTCCGTGACGTCATTCTCGGCGTGTCCGACGGTCTCACTGTCCCTTTTGCCCTCGCCGCGGGGTTGTCCGGCGCTGACGTCAGCTCCTCCATTATCCTCATTGCCGGCATTGCTGAGGTTGCCGCCGGTGCCATCTCCATGGGACTTGGCGGGTATCTTGCGGCGAAGAGTGAAGAAGATCATTACAAGAAAGAACTAAAGAGGGAGCAAGATGAAATCATCGCCGTCCCGGATATAGGACTCGACAAGCCAGACCCAATGAGAGCGCTGCAGAGTGCAATGACAATAGCCTTTTCATACGTCTGTGGTGGACTGGTGCCTCTCCTTCCCTACATGCTCATTCCAGTTGCTACAAAGGCGGTGCTTGCATCTGTGGTGGTCACCATAGTGGCATTGCTGATCTTCGGCTTTGCAAAGGGTCACTTCACAGGAACTCAACCTTTCAAGAGCGCTTTCCAAACCGCCTTCATTGGAGCCATCGCATCCGCCGCAGCTTATTCCATTGCTAAACTCTTCCAATCCTACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

208

Amino Acids

21.99

Weight (kDa)

5.26

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 33 - 110 1.4e-29 VIT family
VIT1 PF01988 113 - 201 5.1e-22 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 108, 177
AccB1I GGYRCC 2 cut(s) 224, 412
AccII CGCG 1 cut(s) 155
AciI CCGC 9 cut(s) 153, 155, 219, 246, 257, 455, 558, 582, 585
AcyI GRCGYC 2 cut(s) 105, 174
AfeI AGCGCT 2 cut(s) 363, 547
AfiI CCNNNNNNNGG 2 cut(s) 154, 332
AgsI TTSAA 1 cut(s) 541
AleI CACNNNNGTG 1 cut(s) 482
AluBI AGCT 2 cut(s) 180, 590
AluI AGCT 2 cut(s) 180, 590
Alw26I GTCTC 1 cut(s) 134
AlwNI CAGNNNCTG 1 cut(s) 49
Aor51HI AGCGCT 2 cut(s) 363, 547
ApeKI GCWGC 2 cut(s) 364, 587
Asp700I GAANNNNTTC 1 cut(s) 77
AspLEI GCGC 3 cut(s) 170, 364, 548
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 41, 469
BanI GGYRCC 2 cut(s) 224, 412
BbvCI CCTCAGC 1 cut(s) 210
BbvI GCAGC 2 cut(s) 351, 599
BccI CCATC 2 cut(s) 236, 581
BceAI ACGGC 1 cut(s) 305
BclI TGATCA 1 cut(s) 10
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 134
BfmI CTRYAG 1 cut(s) 365
BfoI RGCGCY 3 cut(s) 171, 365, 549
BisI GCNGC 6 cut(s) 153, 219, 258, 365, 585, 588
BlsI GCNGC 6 cut(s) 154, 220, 259, 366, 586, 589
Bme1390I CCNGG 1 cut(s) 326
BmiI GGNNCC 3 cut(s) 226, 414, 571
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 3 cut(s) 36, 473, 587
Bpu10I CCTNAGC 1 cut(s) 210
BpuMI CCSGG 1 cut(s) 326
BsaHI GRCGYC 2 cut(s) 105, 174
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 153, 234
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 332
Bse118I RCCGGY 2 cut(s) 200, 221
Bse1I ACTGG 3 cut(s) 87, 414, 440
Bse3DI GCAATG 5 cut(s) 195, 204, 381, 488, 597
BseDI CCNNGG 2 cut(s) 153, 234
BseGI GGATG 1 cut(s) 578
BseLI CCNNNNNNNGG 2 cut(s) 154, 332
BseMI GCAATG 5 cut(s) 195, 204, 381, 488, 597
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 3 cut(s) 87, 414, 440
BseRI GAGGAG 1 cut(s) 172
BseXI GCAGC 2 cut(s) 351, 599
Bsh1236I CGCG 1 cut(s) 155
BshNI GGYRCC 2 cut(s) 224, 412
BsiSI CCGG 4 cut(s) 165, 201, 222, 326
BslFI GGGAC 3 cut(s) 122, 252, 308
BslI CCNNNNNNNGG 2 cut(s) 154, 332
BsmAI GTCTC 1 cut(s) 134
BsmFI GGGAC 3 cut(s) 122, 252, 308
Bso31I GGTCTC 1 cut(s) 134
Bsp143I GATC 3 cut(s) 10, 274, 495
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 9 cut(s) 153, 155, 219, 246, 257, 455, 558, 582, 585
BspCNI CTCAG 1 cut(s) 202
BspFNI CGCG 1 cut(s) 155
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 3 cut(s) 226, 414, 571
BspMAI CTGCAG 1 cut(s) 369
BspT107I GGYRCC 2 cut(s) 224, 412
BspTNI GGTCTC 1 cut(s) 134
BsrDI GCAATG 5 cut(s) 195, 204, 381, 488, 597
BsrFI RCCGGY 2 cut(s) 200, 221
BsrI ACTGG 3 cut(s) 87, 414, 440
BssAI RCCGGY 2 cut(s) 200, 221
BssECI CCNNGG 2 cut(s) 153, 234
BssMI GATC 3 cut(s) 10, 274, 495
BssNI GRCGYC 2 cut(s) 105, 174
BssT1I CCWWGG 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 128, 136
Bst6I CTCTTC 3 cut(s) 63, 257, 614
BstACI GRCGYC 2 cut(s) 105, 174
BstC8I GCNNGC 2 cut(s) 202, 462
BstDEI CTNAG 1 cut(s) 210
BstDSI CCRYGG 2 cut(s) 153, 234
BstEII GGTNACC 1 cut(s) 475
BstF5I GGATG 1 cut(s) 578
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 3 cut(s) 171, 365, 549
BstHHI GCGC 3 cut(s) 170, 364, 548
BstKTI GATC 3 cut(s) 13, 277, 498
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 3 cut(s) 10, 274, 495
BstMWI GCNNNNNNNGC 7 cut(s) 152, 206, 215, 224, 452, 461, 584
BstNSI RCATGY 1 cut(s) 433
BstPI GGTNACC 1 cut(s) 475
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 1 cut(s) 365
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 2 cut(s) 351, 599
BstXI CCANNNNNNTGG 1 cut(s) 235
BtgI CCRYGG 2 cut(s) 153, 234
BtgZI GCGATG 2 cut(s) 301, 559
BtsCI GGATG 1 cut(s) 578
BtsIMutI CAGTG 2 cut(s) 94, 132
Cac8I GCNNGC 2 cut(s) 202, 462
CaiI CAGNNNCTG 1 cut(s) 49
CciI TCATGA 1 cut(s) 13
CfoI GCGC 3 cut(s) 170, 364, 548
Cfr10I RCCGGY 2 cut(s) 200, 221
Cfr42I CCGCGG 1 cut(s) 156
CviAII CATG 4 cut(s) 14, 82, 235, 430
CviJI RGCY 7 cut(s) 24, 180, 346, 386, 504, 572, 590
CviKI_1 RGCY 7 cut(s) 24, 180, 346, 386, 504, 572, 590
DdeI CTNAG 1 cut(s) 210
DpnI GATC 3 cut(s) 12, 276, 497
DpnII GATC 3 cut(s) 10, 274, 495
Eam1104I CTCTTC 3 cut(s) 63, 257, 614
EarI CTCTTC 3 cut(s) 63, 257, 614
EciI GGCGGA 1 cut(s) 571
Eco130I CCWWGG 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 134
Eco47III AGCGCT 2 cut(s) 363, 547
Eco91I GGTNACC 1 cut(s) 475
EcoO65I GGTNACC 1 cut(s) 475
EcoT14I CCWWGG 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 4 cut(s) 17, 85, 238, 433
FaiI YATR 8 cut(s) 15, 27, 83, 236, 332, 394, 431, 482
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FaqI GGGAC 3 cut(s) 122, 252, 308
FatI CATG 4 cut(s) 13, 81, 234, 429
FauI CCCGC 2 cut(s) 148, 239
FbaI TGATCA 1 cut(s) 10
Fnu4HI GCNGC 6 cut(s) 153, 219, 258, 365, 585, 588
FokI GGATG 1 cut(s) 565
Fsp4HI GCNGC 6 cut(s) 153, 219, 258, 365, 585, 588
GlaI GCGC 3 cut(s) 169, 363, 547
GluI GCNGC 6 cut(s) 153, 219, 258, 365, 585, 588
HaeII RGCGCY 3 cut(s) 171, 365, 549
HapII CCGG 4 cut(s) 165, 201, 222, 326
HhaI GCGC 3 cut(s) 170, 364, 548
Hin1I GRCGYC 2 cut(s) 105, 174
Hin1II CATG 4 cut(s) 17, 85, 238, 433
Hin6I GCGC 3 cut(s) 168, 362, 546
HinP1I GCGC 3 cut(s) 168, 362, 546
HinfI GANTC 1 cut(s) 336
HpaII CCGG 4 cut(s) 165, 201, 222, 326
HphI GGTGA 2 cut(s) 41, 469
Hpy166II GTNNAC 1 cut(s) 407
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 3 cut(s) 14, 59, 541
Hpy8I GTNNAC 1 cut(s) 407
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 2 cut(s) 431, 571
HpyCH4III ACNGT 2 cut(s) 128, 136
HpyCH4IV ACGT 3 cut(s) 105, 174, 396
HpyCH4V TGCA 4 cut(s) 367, 374, 464, 509
HpyF10VI GCNNNNNNNGC 7 cut(s) 152, 206, 215, 224, 452, 461, 584
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 3 cut(s) 105, 174, 396
Hsp92I GRCGYC 2 cut(s) 105, 174
Hsp92II CATG 4 cut(s) 17, 85, 238, 433
HspAI GCGC 3 cut(s) 168, 362, 546
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 10
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 3 cut(s) 10, 274, 495
LmnI GCTCC 3 cut(s) 185, 301, 569
Lsp1109I GCAGC 2 cut(s) 351, 599
LweI GCATC 3 cut(s) 36, 473, 587
MaeII ACGT 3 cut(s) 105, 174, 396
MaeIII GTNAC 4 cut(s) 29, 101, 475, 515
MalI GATC 3 cut(s) 12, 276, 497
MboI GATC 3 cut(s) 10, 274, 495
MboII GAAGA 7 cut(s) 47, 80, 274, 281, 284, 490, 601
MlyI GAGTC 1 cut(s) 330
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 6 cut(s) 158, 193, 203, 205, 291, 426
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 77
MslI CAYNNNNRTG 3 cut(s) 18, 233, 482
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 4 cut(s) 165, 201, 222, 326
MspR9I CCNGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 7 cut(s) 152, 206, 215, 224, 452, 461, 584
NaeI GCCGGC 1 cut(s) 202
NciI CCSGG 1 cut(s) 326
NcoI CCATGG 1 cut(s) 234
NdeII GATC 3 cut(s) 10, 274, 495
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 4 cut(s) 17, 85, 238, 433
NlaIV GGNNCC 3 cut(s) 226, 414, 571
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 4 cut(s) 29, 101, 475, 515
NspI RCATGY 1 cut(s) 433
OliI CACNNNNGTG 1 cut(s) 482
PagI TCATGA 1 cut(s) 13
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 77
PfoI TCCNGGA 1 cut(s) 324
PkrI GCNGC 6 cut(s) 154, 220, 259, 366, 586, 589
PleI GAGTC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 330
PspEI GGTNACC 1 cut(s) 475
PspN4I GGNNCC 3 cut(s) 226, 414, 571
PstI CTGCAG 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 49
RseI CAYNNNNRTG 3 cut(s) 18, 233, 482
SacII CCGCGG 1 cut(s) 156
SatI GCNGC 6 cut(s) 153, 219, 258, 365, 585, 588
Sau3AI GATC 3 cut(s) 10, 274, 495
SchI GAGTC 1 cut(s) 330
ScrFI CCNGG 1 cut(s) 326
SetI ASST 7 cut(s) 108, 177, 182, 216, 399, 538, 592
SfaNI GCATC 3 cut(s) 36, 473, 587
SfcI CTRYAG 1 cut(s) 365
Sfr303I CCGCGG 1 cut(s) 156
SgrAI CRCCGGYG 1 cut(s) 221
SgrBI CCGCGG 1 cut(s) 156
SmiMI CAYNNNNRTG 3 cut(s) 18, 233, 482
SsiI CCGC 9 cut(s) 153, 155, 219, 246, 257, 455, 558, 582, 585
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 2 cut(s) 128, 136
TaiI ACGT 3 cut(s) 108, 177, 399
TaqI TCGA 1 cut(s) 339
TauI GCSGC 4 cut(s) 155, 221, 260, 587
TscAI CASTG 2 cut(s) 94, 139
TseFI GTSAC 4 cut(s) 29, 101, 475, 515
TseI GCWGC 2 cut(s) 364, 587
Tsp45I GTSAC 4 cut(s) 29, 101, 475, 515
TspDTI ATGAA 4 cut(s) 70, 324, 381, 553
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 2 cut(s) 94, 139
XceI RCATGY 1 cut(s) 433
XmnI GAANNNNTTC 1 cut(s) 77
ZraI GACGTC 2 cut(s) 106, 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.