MD16G1016500.v1.1

Vacuolar iron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002581
Physical Location & Seq
Forward (+)
1240143 .. 1242202
2060 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1016500.v1.1.491

Sequence Viewer

Length: 747 bp
ATGGCAGATCAAAATGGTTATGCTGACCTAGAGAAGCAGATGCTGATTCTGCATGACCATGAAGAGAAGCACTTCATGTCGAGTGAGATTGTGCGTGACGTCATTCTCGGTGTATCCGACGGTCTCACTGTCCCTTTTGCCCTAGCCGCTGGCTTGTCCGGTGCCGATGTTAGCTCCTCCATCATTCTCATTGCCGGCATTGCTGAAGTTGCCGCCGGTGCAATATCTATGGGACTTGGCGGGTACCTTGCTGCTGCGAGTGAAGCTGATCATTACAAGAAAGAACTAAAGAGGGAACAAGATGAAATCATCGCTGTCCCTGATACAGAGGCTGCTGAGGTTGCTGAAATATTGTCACAATATGGAGTTGAACCACATGAATATGGGCCTGTGGTGAATGCTCTCAGGAGGAACCCTCAAGCCTGGCTTGATTTCATGATGAAATTTGAACTAGGGCTGGAAAAACCAGACCCAATGAGAGCACTACAAAGTGCAATGACAATTGCGTTTTCATACACTGTTGGTGGATTGGTTCCCCTAATACCCTACATGCTCATTCCAATTGCAACACGAGCTGTGGTTGCATCTGTAGTCGTCACCATACTTGCTCTGATGATATTTGGGTTTGCAAAGGGTCACTTCACCGGCACTCAACCCTTCAAGAGCGCCGTCCAAACCGCCTTCATCGGAGCCATTGCCTCCGCCGCTGCTTATTCCATTGCTAAACTTTTCCAAGCTTACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VIT1

Protein Analysis

249

Amino Acids

26.52

Weight (kDa)

5.0

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 31 - 241 1.3e-73 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 102
Acc65I GGTACC 1 cut(s) 243
AccB1I GGYRCC 2 cut(s) 161, 243
AciI CCGC 6 cut(s) 147, 213, 240, 678, 702, 705
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 1 cut(s) 245
AgsI TTSAA 3 cut(s) 371, 449, 661
AjnI CCWGG 1 cut(s) 422
AluBI AGCT 4 cut(s) 174, 266, 575, 737
AluI AGCT 4 cut(s) 174, 266, 575, 737
Alw21I GWGCWC 1 cut(s) 484
Alw26I GTCTC 1 cut(s) 128
AlwNI CAGNNNCTG 2 cut(s) 43, 332
AoxI GGCC 1 cut(s) 386
ApeKI GCWGC 4 cut(s) 251, 254, 332, 707
ApoI RAATTY 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 71
Asp718I GGTACC 1 cut(s) 243
AspLEI GCGC 1 cut(s) 668
AspS9I GGNCC 1 cut(s) 386
AsuHPI GGTGA 3 cut(s) 406, 589, 634
BanI GGYRCC 2 cut(s) 161, 243
BauI CACGAG 1 cut(s) 570
Bbv12I GWGCWC 1 cut(s) 484
BbvCI CCTCAGC 1 cut(s) 336
BbvI GCAGC 4 cut(s) 238, 241, 319, 694
BccI CCATC 1 cut(s) 188
BceAI ACGGC 1 cut(s) 653
BciT130I CCWGG 1 cut(s) 424
BciVI GTATCC 1 cut(s) 124
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 3 cut(s) 29, 143, 452
BfmI CTRYAG 1 cut(s) 588
BfoI RGCGCY 1 cut(s) 669
BfuI GTATCC 1 cut(s) 124
BisI GCNGC 7 cut(s) 147, 213, 252, 255, 333, 705, 708
BlsI GCNGC 7 cut(s) 148, 214, 253, 256, 334, 706, 709
Bme1390I CCNGG 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 5 cut(s) 163, 245, 413, 534, 691
BmrFI CCNGG 1 cut(s) 424
BmsI GCATC 2 cut(s) 30, 593
BplI GAGNNNNNCTC 2 cut(s) 400, 432
Bpu10I CCTNAGC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 128
BsaWI WCCGGW 1 cut(s) 158
Bse118I RCCGGY 3 cut(s) 194, 215, 644
Bse3DI GCAATG 5 cut(s) 189, 198, 501, 693, 717
BseBI CCWGG 1 cut(s) 424
BseMI GCAATG 5 cut(s) 189, 198, 501, 693, 717
BseMII CTCAG 2 cut(s) 327, 418
BseRI GAGGAG 1 cut(s) 166
BseXI GCAGC 4 cut(s) 238, 241, 319, 694
BshFI GGCC 1 cut(s) 388
BshNI GGYRCC 2 cut(s) 161, 243
BsiHKAI GWGCWC 1 cut(s) 484
BsiSI CCGG 4 cut(s) 159, 195, 216, 645
BslFI GGGAC 3 cut(s) 116, 246, 302
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 3 cut(s) 116, 246, 302
BsmI GAATGC 1 cut(s) 403
BsnI GGCC 1 cut(s) 388
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 484
Bsp143I GATC 2 cut(s) 7, 268
BspACI CCGC 6 cut(s) 147, 213, 240, 678, 702, 705
BspANI GGCC 1 cut(s) 388
BspCNI CTCAG 2 cut(s) 328, 417
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 5 cut(s) 163, 245, 413, 534, 691
BspT107I GGYRCC 2 cut(s) 161, 243
BspTNI GGTCTC 1 cut(s) 128
BsrDI GCAATG 5 cut(s) 189, 198, 501, 693, 717
BsrFI RCCGGY 3 cut(s) 194, 215, 644
BssAI RCCGGY 3 cut(s) 194, 215, 644
BssMI GATC 2 cut(s) 7, 268
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 1 cut(s) 570
Bst2BI CACGAG 1 cut(s) 570
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 3 cut(s) 122, 130, 520
Bst6I CTCTTC 1 cut(s) 57
BstACI GRCGYC 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 151, 196
BstDEI CTNAG 2 cut(s) 336, 404
BstH2I RGCGCY 1 cut(s) 669
BstHHI GCGC 1 cut(s) 668
BstKTI GATC 2 cut(s) 10, 271
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 2 cut(s) 7, 268
BstNI CCWGG 1 cut(s) 424
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 1 cut(s) 422
BstSFI CTRYAG 1 cut(s) 588
BstV1I GCAGC 4 cut(s) 238, 241, 319, 694
BsuI GTATCC 1 cut(s) 124
BsuRI GGCC 1 cut(s) 388
BtgZI GCGATG 1 cut(s) 295
BtsIMutI CAGTG 2 cut(s) 126, 516
Cac8I GCNNGC 2 cut(s) 151, 196
CaiI CAGNNNCTG 2 cut(s) 43, 332
CciI TCATGA 1 cut(s) 435
CfoI GCGC 1 cut(s) 668
Cfr10I RCCGGY 3 cut(s) 194, 215, 644
Cfr13I GGNCC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 244
CviAII CATG 6 cut(s) 53, 59, 76, 377, 436, 550
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 2 cut(s) 336, 404
DpnI GATC 2 cut(s) 9, 270
DpnII GATC 2 cut(s) 7, 268
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
EciI GGCGGA 1 cut(s) 691
Eco31I GGTCTC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 422
FaeI CATG 6 cut(s) 56, 62, 79, 380, 439, 553
FalI AAGNNNNNCTT 4 cut(s) 411, 443, 623, 655
FaqI GGGAC 3 cut(s) 116, 246, 302
FatI CATG 6 cut(s) 52, 58, 75, 376, 435, 549
FauI CCCGC 1 cut(s) 233
FbaI TGATCA 1 cut(s) 268
Fnu4HI GCNGC 7 cut(s) 147, 213, 252, 255, 333, 705, 708
Fsp4HI GCNGC 7 cut(s) 147, 213, 252, 255, 333, 705, 708
FspBI CTAG 3 cut(s) 29, 143, 452
GlaI GCGC 1 cut(s) 667
GluI GCNGC 7 cut(s) 147, 213, 252, 255, 333, 705, 708
HaeII RGCGCY 1 cut(s) 669
HaeIII GGCC 1 cut(s) 388
HapII CCGG 4 cut(s) 159, 195, 216, 645
HhaI GCGC 1 cut(s) 668
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 6 cut(s) 56, 62, 79, 380, 439, 553
Hin6I GCGC 1 cut(s) 666
HinP1I GCGC 1 cut(s) 666
HindIII AAGCTT 1 cut(s) 735
HinfI GANTC 1 cut(s) 46
HpaII CCGG 4 cut(s) 159, 195, 216, 645
HphI GGTGA 3 cut(s) 406, 589, 634
Hpy188I TCNGA 3 cut(s) 118, 612, 689
Hpy188III TCNNGA 3 cut(s) 406, 436, 661
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 2 cut(s) 667, 691
HpyCH4III ACNGT 3 cut(s) 122, 130, 520
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 6 cut(s) 52, 221, 494, 566, 584, 629
HpyF3I CTNAG 2 cut(s) 336, 404
HpySE526I ACGT 1 cut(s) 99
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 6 cut(s) 56, 62, 79, 380, 439, 553
HspAI GCGC 1 cut(s) 666
KpnI GGTACC 1 cut(s) 247
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 2 cut(s) 7, 268
LmnI GCTCC 2 cut(s) 179, 689
Lsp1109I GCAGC 4 cut(s) 238, 241, 319, 694
LweI GCATC 2 cut(s) 30, 593
MaeI CTAG 3 cut(s) 29, 143, 452
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 4 cut(s) 95, 354, 595, 635
MalI GATC 2 cut(s) 9, 270
MboI GATC 2 cut(s) 7, 268
MboII GAAGA 1 cut(s) 74
MfeI CAATTG 2 cut(s) 501, 561
MhlI GDGCHC 1 cut(s) 484
MluCI AATT 3 cut(s) 443, 501, 561
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 7 cut(s) 187, 285, 322, 331, 402, 426, 709
MroNI GCCGGC 1 cut(s) 194
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 1 cut(s) 745
MslI CAYNNNNRTG 2 cut(s) 57, 381
MspA1I CMGCKG 2 cut(s) 149, 707
MspI CCGG 4 cut(s) 159, 195, 216, 645
MspR9I CCNGG 1 cut(s) 424
MunI CAATTG 2 cut(s) 501, 561
Mva1269I GAATGC 1 cut(s) 403
MvaI CCWGG 1 cut(s) 424
NaeI GCCGGC 1 cut(s) 196
NdeII GATC 2 cut(s) 7, 268
NgoMIV GCCGGC 1 cut(s) 194
NlaIII CATG 6 cut(s) 56, 62, 79, 380, 439, 553
NlaIV GGNNCC 5 cut(s) 163, 245, 413, 534, 691
NmuCI GTSAC 4 cut(s) 95, 354, 595, 635
NspI RCATGY 1 cut(s) 553
PagI TCATGA 1 cut(s) 435
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 1 cut(s) 403
PdiI GCCGGC 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 46
PkrI GCNGC 7 cut(s) 148, 214, 253, 256, 334, 706, 709
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspN4I GGNNCC 5 cut(s) 163, 245, 413, 534, 691
PspPI GGNCC 1 cut(s) 386
PstNI CAGNNNCTG 2 cut(s) 43, 332
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 2 cut(s) 57, 381
SaqAI TTAA 1 cut(s) 745
SatI GCNGC 7 cut(s) 147, 213, 252, 255, 333, 705, 708
Sau3AI GATC 2 cut(s) 7, 268
Sau96I GGNCC 1 cut(s) 386
ScrFI CCNGG 1 cut(s) 424
SduI GDGCHC 1 cut(s) 484
SetI ASST 8 cut(s) 30, 102, 176, 249, 268, 342, 577, 739
SfaNI GCATC 2 cut(s) 30, 593
SfcI CTRYAG 1 cut(s) 588
SgrAI CRCCGGYG 1 cut(s) 215
SmiMI CAYNNNNRTG 2 cut(s) 57, 381
SmlI CTYRAG 1 cut(s) 417
SmoI CTYRAG 1 cut(s) 417
Sse9I AATT 3 cut(s) 443, 501, 561
SsiI CCGC 6 cut(s) 147, 213, 240, 678, 702, 705
SspI AATATT 1 cut(s) 351
SspMI CTAG 3 cut(s) 29, 143, 452
StyD4I CCNGG 1 cut(s) 422
TaaI ACNGT 3 cut(s) 122, 130, 520
TaiI ACGT 1 cut(s) 102
TaqI TCGA 1 cut(s) 80
TasI AATT 3 cut(s) 443, 501, 561
TauI GCSGC 3 cut(s) 149, 215, 707
TfiI GAWTC 1 cut(s) 46
Tru1I TTAA 1 cut(s) 745
Tru9I TTAA 1 cut(s) 745
TscAI CASTG 2 cut(s) 133, 523
TseFI GTSAC 4 cut(s) 95, 354, 595, 635
TseI GCWGC 4 cut(s) 251, 254, 332, 707
Tsp45I GTSAC 4 cut(s) 95, 354, 595, 635
TspDTI ATGAA 8 cut(s) 64, 75, 318, 393, 424, 455, 501, 673
TspRI CASTG 2 cut(s) 133, 523
XapI RAATTY 1 cut(s) 443
XceI RCATGY 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 3 cut(s) 29, 143, 452
ZraI GACGTC 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.