FvH4_4g36560

BUD13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010092
Physical Location & Seq
Reverse (-)
33446883 .. 33453324
6442 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36560.t1

Sequence Viewer

Length: 1449 bp
ATGGCGGATTCCGCGAAATCGCTCAAAGAGTATTTGAAACGATACGAGTCTAGTGACAACGCGGACGAGACGAAGAAGAAGAGGAAGAAGAAGCAGAAGAGCAGGACGGAGGCCATGGGGGTTAGAGTGGTGGATGAAGATCCTGTGTGGCAGAAACCGGTGAAAATCAAGGGAGAAGATGAGAAAGATTCGGGAGACGAAGAGGAGGAGAAGCCGTTGGTGGATGAAGACATTGAGGTGAAGAGGATGAGGAGGCTTGAGTTGTTGAGAGCGCGGCGGCCGTATGATGCTATATCTGAAGATGGCAGTGGTTGGGTTCAAGTCCCTCTGAAAAGAGAAGGCTCCGAAGAAGCTGACTTGTCGCCTCCGCGGAAGCGGTTTCGTTCTCCCTCTCCCGAGCCTGGGATGGCTTCGAGGGGATCAGACCTTTCGCCCCCGAGGAAAAGTAGAAGGGGCGGTGATGAAGATCTGTCTCCACCGAGGAAGAGTAGGAACAATGTTGAGGATCTTTCGCCACCGAGAAAGAGTAGGAACAGTGTTGCAGATCTTTCTCCCCCGAGGAAGAGTAGGAACAATGTTGAGGATCTTTCGCCACCAAGGAAGAGTAGGAACAATGTTGAAGATCTTTCTCCACCAAGGAAGAGACGGATCAATGGTGGTGAGGATCTTTCTCCACCAAGGAAGAGACAGAACAATGTTGCAGATCTTTCTCCACCGAGGAAGAGTAGGATCAATGGTGATGCGGATCTTTCTCCACCAAGGAAAAAGAAGCAATCATCTCCAGGTCCTGTAAAAGAGAAGCCAAAAAGTGGTTTGATATCTGGCCGAGATATCAGCGAAGAGATTTCTAAAAAGAAGAGGGCTGAGCAGTTGAGATTTCAACAGATGGATCCTTCTGAAACTGGGCAAAATGCAGCAAATGTATTTCGTAATAAGAAAACAGGTGAACGCATGTCGGAGGAGGAGAAATTGAAATCAGAGAAGAAACGTGATGAAAAGCCTAAGGAGATAAAGGTTGAATGGGGTAAGGGCTTGGCTCAAAAGCGGGAAGCTGAAGCTACGCTGGAGGCATTAGAACGTGAGAAGGAGAAACCATTTGCACGGACTAGAGATGATCCAGAACTTGACAAAATGATGAAGGAGAAAGTAAGATGGGGTGACCCTATGGCGCATTTGGTGAAGAAAAAGTTTCCTGAAGCAGTTCTTGCGAACCTAGGGGACAGCGATAAAATGAAGGAATCTGGATTTATCATCCCTCAGGATATTCCTAATCATAGCTGGTTGAAAAGAGGGTTAGATGCTGCACCAAATCGTTATGGTATAAAACCAGGGAGACATTGGGATGGAGTTGATCGCAGTAATGGATTTGAGAAGAAACTGTTCACGCAAATAAACGAGAAACAAGCTACTCAGGCAGAGGCGTATCTGTGGTCCGTGGCAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bud13

Protein Analysis

483

Amino Acids

55.03

Weight (kDa)

9.66

Isoelectric Point (pI)

64.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bud13 PF09736 308 - 465 1.2e-39 Pre-mRNA-splicing factor of RES complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 809
AccII CGCG 4 cut(s) 14, 62, 274, 370
AcoI YGGCCR 2 cut(s) 278, 823
AcuI CTGAAG 3 cut(s) 318, 1074, 1215
AfiI CCNNNNNNNGG 3 cut(s) 401, 402, 809
AgeI ACCGGT 1 cut(s) 157
AgsI TTSAA 7 cut(s) 37, 320, 620, 881, 973, 1019, 1285
AjnI CCWGG 3 cut(s) 400, 781, 1327
AluBI AGCT 5 cut(s) 353, 1052, 1058, 1278, 1406
AluI AGCT 5 cut(s) 353, 1052, 1058, 1278, 1406
Alw26I GTCTC 6 cut(s) 62, 189, 477, 637, 679, 1327
AlwNI CAGNNNCTG 1 cut(s) 788
Ama87I CYCGRG 3 cut(s) 395, 436, 556
AoxI GGCC 3 cut(s) 111, 278, 823
ApeKI GCWGC 2 cut(s) 914, 1301
AsiGI ACCGGT 1 cut(s) 157
Asp700I GAANNNNTTC 2 cut(s) 1200, 1379
AspA2I CCTAGG 1 cut(s) 1213
AspLEI GCGC 2 cut(s) 274, 1171
AspS9I GGNCC 2 cut(s) 785, 1431
AsuHPI GGTGA 8 cut(s) 172, 250, 470, 671, 749, 956, 1169, 1189
AvaI CYCGRG 3 cut(s) 395, 436, 556
AvaII GGWCC 2 cut(s) 785, 1431
AvrII CCTAGG 1 cut(s) 1213
AxyI CCTNAGG 2 cut(s) 1002, 1257
BamHI GGATCC 1 cut(s) 889
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 2 cut(s) 926, 1288
BccI CCATC 5 cut(s) 296, 400, 880, 1146, 1337
BceAI ACGGC 2 cut(s) 199, 265
BciT130I CCWGG 3 cut(s) 402, 783, 1329
BcoDI GTCTC 6 cut(s) 62, 189, 477, 637, 679, 1327
BfaI CTAG 3 cut(s) 51, 1107, 1214
BglII AGATCT 4 cut(s) 466, 544, 622, 703
BisI GCNGC 4 cut(s) 275, 278, 915, 1302
BlnI CCTAGG 1 cut(s) 1213
BlpI GCTNAGC 1 cut(s) 864
BlsI GCNGC 4 cut(s) 276, 279, 916, 1303
Bme1390I CCNGG 3 cut(s) 402, 783, 1329
Bme18I GGWCC 2 cut(s) 785, 1431
BmeT110I CYCGRG 3 cut(s) 395, 436, 556
BmgT120I GGNCC 2 cut(s) 785, 1431
BmiI GGNNCC 2 cut(s) 343, 891
BmrFI CCNGG 3 cut(s) 402, 783, 1329
BmrI ACTGGG 1 cut(s) 912
BmsI GCATC 3 cut(s) 277, 730, 1288
BmuI ACTGGG 1 cut(s) 912
BpiI GAAGAC 1 cut(s) 234
BpmI CTGGAG 2 cut(s) 765, 1085
Bpu1102I GCTNAGC 1 cut(s) 864
BpuEI CTTGAG 1 cut(s) 278
BsaBI GATNNNNATC 3 cut(s) 138, 465, 744
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 402, 809
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 907
Bse21I CCTNAGG 2 cut(s) 1002, 1257
Bse8I GATNNNNATC 3 cut(s) 138, 465, 744
BseBI CCWGG 3 cut(s) 402, 783, 1329
BseGI GGATG 6 cut(s) 139, 229, 252, 411, 1251, 1348
BseJI GATNNNNATC 3 cut(s) 138, 465, 744
BseLI CCNNNNNNNGG 3 cut(s) 401, 402, 809
BseMII CTCAG 3 cut(s) 855, 1271, 1424
BseNI ACTGG 1 cut(s) 907
BseRI GAGGAG 5 cut(s) 218, 221, 265, 974, 977
BseX3I CGGCCG 1 cut(s) 278
BseXI GCAGC 2 cut(s) 926, 1288
BsgI GTGCAG 1 cut(s) 1287
Bsh1236I CGCG 4 cut(s) 14, 62, 274, 370
Bsh1285I CGRYCG 1 cut(s) 281
BshFI GGCC 3 cut(s) 113, 280, 825
BshTI ACCGGT 1 cut(s) 157
BsiEI CGRYCG 1 cut(s) 281
BsiHKCI CYCGRG 3 cut(s) 395, 436, 556
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 2 cut(s) 308, 1232
BslI CCNNNNNNNGG 3 cut(s) 401, 402, 809
BsmAI GTCTC 6 cut(s) 62, 189, 477, 637, 679, 1327
BsmBI CGTCTC 3 cut(s) 62, 189, 637
BsmFI GGGAC 2 cut(s) 308, 1232
BsnI GGCC 3 cut(s) 113, 280, 825
BsoBI CYCGRG 3 cut(s) 395, 436, 556
Bsp1720I GCTNAGC 1 cut(s) 864
Bsp19I CCATGG 1 cut(s) 114
BspANI GGCC 3 cut(s) 113, 280, 825
BspCNI CTCAG 3 cut(s) 856, 1270, 1423
BspFNI CGCG 4 cut(s) 14, 62, 274, 370
BspLI GGNNCC 2 cut(s) 343, 891
BspQI GCTCTTC 1 cut(s) 92
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 907
BssAI RCCGGY 1 cut(s) 157
BssT1I CCWWGG 6 cut(s) 114, 596, 635, 677, 758, 1213
Bst2UI CCWGG 3 cut(s) 402, 783, 1329
Bst4CI ACNGT 2 cut(s) 536, 1380
BstAPI GCANNNNNTGC 1 cut(s) 1205
BstDEI CTNAG 4 cut(s) 864, 1002, 1257, 1410
BstDSI CCRYGG 3 cut(s) 114, 368, 1434
BstEII GGTNACC 1 cut(s) 1157
BstF5I GGATG 6 cut(s) 139, 229, 252, 411, 1251, 1348
BstFNI CGCG 4 cut(s) 14, 62, 274, 370
BstHHI GCGC 2 cut(s) 274, 1171
BstMAI GTCTC 6 cut(s) 62, 189, 477, 637, 679, 1327
BstMCI CGRYCG 1 cut(s) 281
BstMWI GCNNNNNNNGC 3 cut(s) 11, 1205, 1412
BstNI CCWGG 3 cut(s) 402, 783, 1329
BstNSI RCATGY 1 cut(s) 955
BstPI GGTNACC 1 cut(s) 1157
BstSCI CCNGG 3 cut(s) 400, 781, 1327
BstUI CGCG 4 cut(s) 14, 62, 274, 370
BstV1I GCAGC 2 cut(s) 926, 1288
BstV2I GAAGAC 1 cut(s) 234
BstZI CGGCCG 1 cut(s) 278
Bsu36I CCTNAGG 2 cut(s) 1002, 1257
BsuRI GGCC 3 cut(s) 113, 280, 825
BtgI CCRYGG 3 cut(s) 114, 368, 1434
BtsCI GGATG 6 cut(s) 139, 229, 252, 411, 1251, 1348
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 2 cut(s) 313, 541
CaiI CAGNNNCTG 1 cut(s) 788
CfoI GCGC 2 cut(s) 274, 1171
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 785, 1431
Cfr42I CCGCGG 1 cut(s) 371
CspAI ACCGGT 1 cut(s) 157
CviAII CATG 2 cut(s) 115, 952
DdeI CTNAG 4 cut(s) 864, 1002, 1257, 1410
EaeI YGGCCR 2 cut(s) 278, 823
EagI CGGCCG 1 cut(s) 278
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 278
Eco130I CCWWGG 6 cut(s) 114, 596, 635, 677, 758, 1213
Eco32I GATATC 2 cut(s) 819, 832
Eco47I GGWCC 2 cut(s) 785, 1431
Eco52I CGGCCG 1 cut(s) 278
Eco57I CTGAAG 3 cut(s) 318, 1074, 1215
Eco81I CCTNAGG 2 cut(s) 1002, 1257
Eco88I CYCGRG 3 cut(s) 395, 436, 556
Eco91I GGTNACC 1 cut(s) 1157
EcoO109I RGGNCCY 1 cut(s) 785
EcoO65I GGTNACC 1 cut(s) 1157
EcoRII CCWGG 3 cut(s) 400, 781, 1327
EcoRV GATATC 2 cut(s) 819, 832
EcoT14I CCWWGG 6 cut(s) 114, 596, 635, 677, 758, 1213
ErhI CCWWGG 6 cut(s) 114, 596, 635, 677, 758, 1213
Esp3I CGTCTC 3 cut(s) 62, 189, 637
FaeI CATG 2 cut(s) 118, 955
FaiI YATR 9 cut(s) 116, 285, 293, 953, 1166, 1275, 1317, 1322, 1445
FalI AAGNNNNNCTT 2 cut(s) 1188, 1220
FaqI GGGAC 2 cut(s) 308, 1232
FatI CATG 2 cut(s) 114, 951
FauI CCCGC 1 cut(s) 1038
Fnu4HI GCNGC 4 cut(s) 275, 278, 915, 1302
FokI GGATG 6 cut(s) 146, 236, 259, 418, 1238, 1355
Fsp4HI GCNGC 4 cut(s) 275, 278, 915, 1302
FspBI CTAG 3 cut(s) 51, 1107, 1214
GlaI GCGC 2 cut(s) 273, 1170
GluI GCNGC 4 cut(s) 275, 278, 915, 1302
GsuI CTGGAG 2 cut(s) 765, 1085
HaeIII GGCC 3 cut(s) 113, 280, 825
HapII CCGG 1 cut(s) 158
HhaI GCGC 2 cut(s) 274, 1171
Hin1II CATG 2 cut(s) 118, 955
Hin6I GCGC 2 cut(s) 272, 1169
HinP1I GCGC 2 cut(s) 272, 1169
HinfI GANTC 4 cut(s) 8, 47, 188, 1238
HpaII CCGG 1 cut(s) 158
HphI GGTGA 8 cut(s) 172, 250, 470, 671, 749, 956, 1169, 1189
Hpy166II GTNNAC 2 cut(s) 947, 1383
Hpy188I TCNGA 7 cut(s) 298, 330, 346, 424, 898, 958, 979
Hpy188III TCNNGA 6 cut(s) 192, 395, 1118, 1193, 1242, 1259
Hpy8I GTNNAC 2 cut(s) 947, 1383
HpyAV CCTTC 6 cut(s) 332, 444, 903, 1078, 1132, 1228
HpyCH4III ACNGT 2 cut(s) 536, 1380
HpyCH4IV ACGT 2 cut(s) 988, 1078
HpyCH4V TGCA 5 cut(s) 542, 701, 914, 1100, 1304
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 1205, 1412
HpyF3I CTNAG 4 cut(s) 864, 1002, 1257, 1410
HpySE526I ACGT 2 cut(s) 988, 1078
Hsp92II CATG 2 cut(s) 118, 955
HspAI GCGC 2 cut(s) 272, 1169
KspI CCGCGG 1 cut(s) 371
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 1 cut(s) 347
Lsp1109I GCAGC 2 cut(s) 926, 1288
LweI GCATC 3 cut(s) 277, 730, 1288
MaeI CTAG 3 cut(s) 51, 1107, 1214
MaeII ACGT 2 cut(s) 988, 1078
MaeIII GTNAC 2 cut(s) 53, 1157
MluCI AATT 1 cut(s) 968
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 1 cut(s) 936
MroXI GAANNNNTTC 2 cut(s) 1200, 1379
MslI CAYNNNNRTG 2 cut(s) 236, 1341
MspA1I CMGCKG 1 cut(s) 370
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 3 cut(s) 402, 783, 1329
MvaI CCWGG 3 cut(s) 402, 783, 1329
MvnI CGCG 4 cut(s) 14, 62, 274, 370
MwoI GCNNNNNNNGC 3 cut(s) 11, 1205, 1412
NcoI CCATGG 1 cut(s) 114
NlaIII CATG 2 cut(s) 118, 955
NlaIV GGNNCC 2 cut(s) 343, 891
NmeAIII GCCGAG 1 cut(s) 851
NmuCI GTSAC 2 cut(s) 53, 1157
NspI RCATGY 1 cut(s) 955
PciSI GCTCTTC 1 cut(s) 92
PdmI GAANNNNTTC 2 cut(s) 1200, 1379
PfeI GAWTC 3 cut(s) 8, 188, 1238
PflMI CCANNNNNTGG 1 cut(s) 809
PinAI ACCGGT 1 cut(s) 157
PkrI GCNGC 4 cut(s) 276, 279, 916, 1303
PleI GAGTC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 55
PpuMI RGGWCCY 1 cut(s) 785
Psp5II RGGWCCY 1 cut(s) 785
Psp6I CCWGG 3 cut(s) 400, 781, 1327
PspEI GGTNACC 1 cut(s) 1157
PspGI CCWGG 3 cut(s) 400, 781, 1327
PspN4I GGNNCC 2 cut(s) 343, 891
PspPI GGNCC 2 cut(s) 785, 1431
PspPPI RGGWCCY 1 cut(s) 785
PstNI CAGNNNCTG 1 cut(s) 788
RseI CAYNNNNRTG 2 cut(s) 236, 1341
SacII CCGCGG 1 cut(s) 371
SapI GCTCTTC 1 cut(s) 92
SatI GCNGC 4 cut(s) 275, 278, 915, 1302
Sau96I GGNCC 2 cut(s) 785, 1431
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 3 cut(s) 402, 783, 1329
SfaNI GCATC 3 cut(s) 277, 730, 1288
Sfr303I CCGCGG 1 cut(s) 371
SgrBI CCGCGG 1 cut(s) 371
SinI GGWCC 2 cut(s) 785, 1431
SmiMI CAYNNNNRTG 2 cut(s) 236, 1341
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 1 cut(s) 968
SspMI CTAG 3 cut(s) 51, 1107, 1214
StyD4I CCNGG 3 cut(s) 400, 781, 1327
StyI CCWWGG 6 cut(s) 114, 596, 635, 677, 758, 1213
TaaI ACNGT 2 cut(s) 536, 1380
TaiI ACGT 2 cut(s) 991, 1081
TaqI TCGA 1 cut(s) 413
TasI AATT 1 cut(s) 968
TauI GCSGC 2 cut(s) 277, 280
TfiI GAWTC 3 cut(s) 8, 188, 1238
TscAI CASTG 2 cut(s) 313, 541
TseFI GTSAC 2 cut(s) 53, 1157
TseI GCWGC 2 cut(s) 914, 1301
Tsp45I GTSAC 2 cut(s) 53, 1157
TspDTI ATGAA 6 cut(s) 150, 240, 477, 1008, 1151, 1247
TspGWI ACGGA 4 cut(s) 122, 661, 1117, 1423
TspRI CASTG 2 cut(s) 313, 541
Van91I CCANNNNNTGG 1 cut(s) 809
VpaK11BI GGWCC 2 cut(s) 785, 1431
XceI RCATGY 1 cut(s) 955
XcmI CCANNNNNNNNNTGG 1 cut(s) 1335
XmaJI CCTAGG 1 cut(s) 1213
XmnI GAANNNNTTC 2 cut(s) 1200, 1379
XspI CTAG 3 cut(s) 51, 1107, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.