RLG00000005672

BUD13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0010092
Physical Location & Seq
Forward (+)
737856 .. 741119
3264 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005672

Sequence Viewer

Length: 1443 bp
ATGGCGGCTTCCACGAAATCGCTTAAAGAGTATTTGAAACGATACGAATCTAGTAATAACGAGGACGAGACGAAGAAGAAGAGGAAGAAGAAGCAGAAGAGCAAGGCGGAGGCAATGGGGGTTAAAGTGGTGGATGAAGATCCTGTGTGGCAGAAACCGGTGAAAATTGAGGGAGAAGAAGATAACAATTCAGCCGACGAAGAGGAGAAGCCTTTAGTTGATGAAGACATTGAGGTGAAGAGGATGAAGAGACTTGAGTTATTAAGGGCTCAGCGTCCCTATGATGCTATATCTGAAGATGGCAGTGGTTGGGTTAAAGTCCCTCCGAAACGAGAAGGCTCCGAAGAGGCTGACTTGTCACCTCCACGGAAGCGCTATCATTCTCCCTCACCTGAGCCTGGAATGGCTTCGAGGGGATCAGACCTTTCGCCGCCGAGGAAAAGTAGAAGCAATGTTGAAGATCTTTCTCCACCGAGGAAGAGGAGGAACCATGTTGCGGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGGTGATGGAGATCTTTCTCCACCAAGGAACAGGCGGAACAATGTTGAAGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGTTGCAGATCTTTCTCCACCGAGGAACAGGCGGAACAATGTTGAAGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGTTGCAGATCTTTCTCCACCAAGGAAAAGTAGGAGGGACATTGATGAAGATCTATCTCCACCAAGGAAAAAGAAGCAAGTATCTCCGGGTCCTTTGAAAGAGAAGCCAAAAAGTGGTTTGATTTCTGGCCAAGATATCAGCAAAGAGATTTCTAAAACGAAGAGAGCTGAGCAGTCGAGATTTGAACAGATGGATCCTTCTGAAACTGGGCAACAAGCAGCAGTTGTATTTCGTAATAAGAAAACAGGAGAACGCATGTCGGAGGAGGAGCGATTGAAATCAGAAAAGAAACTTGATGAAAAGCCTAAGGAGATAAAGTTGGAATGGGGTAAGGGCTTGGCTCAAAAGCGGGAAGCTGAAGCTAAGCTAGAGGAATTAGAACGTGAGAAGGAAAAACCATTTGCACGGACTAGAGATGATCCAGAACTTGACAAAATGATGAAGGAGAGAGTAAGATGGGGTGACCCAATGGCACATTTGGTAAAGAAAAAGTATCCTGAAGCAGTTCTCCCTAACCTAGGGGATAGCGAGAAAATGAAGGAATCTGGATTTATTATCCCTCAGGATATTCCTAGTCATAGCTGGTTAAAAAGAGGATTAGATGCTGCGCCAAATCGATATGGTATAAAACCAGGGAGACATTGGGATGGAGTTGATCGCAGTAACGGATTTGAGAAAGGATTGTTCAAACGGACTAACGAGAAACAAGCCACCCAGACGGAGGCTTATCTTTGGTCTGTCGCAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bud13

Protein Analysis

481

Amino Acids

54.99

Weight (kDa)

9.59

Isoelectric Point (pI)

65.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bud13 PF09736 305 - 463 3.2e-40 Pre-mRNA-splicing factor of RES complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 803
AciI CCGC 7 cut(s) 5, 107, 431, 497, 565, 643, 1039
AclWI GGATC 6 cut(s) 134, 424, 507, 878, 891, 1103
AcoI YGGCCR 1 cut(s) 817
AcuI CTGAAG 3 cut(s) 315, 1068, 1209
AfeI AGCGCT 1 cut(s) 374
AfiI CCNNNNNNNGG 5 cut(s) 398, 496, 803, 1208, 1411
AgeI ACCGGT 1 cut(s) 157
AgsI TTSAA 8 cut(s) 37, 458, 578, 656, 787, 875, 967, 1378
AjnI CCWGG 2 cut(s) 397, 1321
AluBI AGCT 5 cut(s) 857, 1046, 1052, 1057, 1272
AluI AGCT 5 cut(s) 857, 1046, 1052, 1057, 1272
Alw26I GTCTC 3 cut(s) 62, 244, 1321
AlwI GGATC 6 cut(s) 134, 424, 507, 878, 891, 1103
Aor51HI AGCGCT 1 cut(s) 374
AoxI GGCC 1 cut(s) 817
ApeKI GCWGC 2 cut(s) 908, 1295
AsiGI ACCGGT 1 cut(s) 157
Asp700I GAANNNNTTC 2 cut(s) 406, 1194
AspA2I CCTAGG 1 cut(s) 1207
AspLEI GCGC 2 cut(s) 375, 1300
AspS9I GGNCC 1 cut(s) 779
AsuC2I CCSGG 1 cut(s) 777
AsuHPI GGTGA 6 cut(s) 172, 247, 351, 381, 545, 1163
AvaII GGWCC 1 cut(s) 779
AvrII CCTAGG 1 cut(s) 1207
AxyI CCTNAGG 2 cut(s) 996, 1251
BalI TGGCCA 1 cut(s) 819
BamHI GGATCC 1 cut(s) 883
BanII GRGCYC 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 920, 1282
BccI CCATC 5 cut(s) 293, 530, 874, 1140, 1331
BciT130I CCWGG 2 cut(s) 399, 1323
BciVI GTATCC 1 cut(s) 1194
BcnI CCSGG 1 cut(s) 777
BcoDI GTCTC 3 cut(s) 62, 244, 1321
BfaI CTAG 5 cut(s) 51, 1058, 1101, 1208, 1263
BfoI RGCGCY 1 cut(s) 376
BfuI GTATCC 1 cut(s) 1194
BglII AGATCT 7 cut(s) 460, 541, 580, 619, 658, 697, 739
BisI GCNGC 4 cut(s) 6, 431, 909, 1296
BlnI CCTAGG 1 cut(s) 1207
BlpI GCTNAGC 3 cut(s) 270, 858, 1053
BlsI GCNGC 4 cut(s) 7, 432, 910, 1297
Bme1390I CCNGG 3 cut(s) 399, 777, 1323
Bme18I GGWCC 1 cut(s) 779
BmgT120I GGNCC 1 cut(s) 779
BmiI GGNNCC 4 cut(s) 340, 488, 780, 885
BmrFI CCNGG 3 cut(s) 399, 777, 1323
BmrI ACTGGG 1 cut(s) 906
BmsI GCATC 2 cut(s) 274, 1282
BmuI ACTGGG 1 cut(s) 906
BpiI GAAGAC 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 393
Bpu1102I GCTNAGC 3 cut(s) 270, 858, 1053
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 777
Bsa29I ATCGAT 1 cut(s) 1306
BsaBI GATNNNNATC 5 cut(s) 46, 138, 540, 738, 967
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 992, 1022
Bsc4I CCNNNNNNNGG 5 cut(s) 398, 496, 803, 1208, 1411
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 901
Bse21I CCTNAGG 2 cut(s) 996, 1251
Bse3DI GCAATG 2 cut(s) 120, 457
Bse8I GATNNNNATC 5 cut(s) 46, 138, 540, 738, 967
BseBI CCWGG 2 cut(s) 399, 1323
BseCI ATCGAT 1 cut(s) 1306
BseGI GGATG 3 cut(s) 139, 249, 1342
BseJI GATNNNNATC 5 cut(s) 46, 138, 540, 738, 967
BseLI CCNNNNNNNGG 5 cut(s) 398, 496, 803, 1208, 1411
BseMI GCAATG 2 cut(s) 120, 457
BseMII CTCAG 4 cut(s) 284, 384, 849, 1265
BseNI ACTGG 1 cut(s) 901
BseRI GAGGAG 4 cut(s) 218, 496, 968, 971
BseXI GCAGC 2 cut(s) 920, 1282
BshFI GGCC 1 cut(s) 819
BshTI ACCGGT 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 1306
BsiSI CCGG 2 cut(s) 158, 776
BslFI GGGAC 3 cut(s) 261, 305, 740
BslI CCNNNNNNNGG 5 cut(s) 398, 496, 803, 1208, 1411
BsmAI GTCTC 3 cut(s) 62, 244, 1321
BsmBI CGTCTC 1 cut(s) 62
BsmFI GGGAC 3 cut(s) 261, 305, 740
BsnI GGCC 1 cut(s) 819
Bsp1286I GDGCHC 1 cut(s) 271
Bsp1720I GCTNAGC 3 cut(s) 270, 858, 1053
BspACI CCGC 7 cut(s) 5, 107, 431, 497, 565, 643, 1039
BspANI GGCC 1 cut(s) 819
BspCNI CTCAG 4 cut(s) 283, 385, 850, 1264
BspDI ATCGAT 1 cut(s) 1306
BspLI GGNNCC 4 cut(s) 340, 488, 780, 885
BspPI GGATC 6 cut(s) 134, 424, 507, 878, 891, 1103
BspQI GCTCTTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 120, 457
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 901
BssAI RCCGGY 1 cut(s) 157
BssT1I CCWWGG 4 cut(s) 554, 710, 752, 1207
Bst2UI CCWGG 2 cut(s) 399, 1323
BstDEI CTNAG 6 cut(s) 270, 393, 858, 996, 1053, 1251
BstDSI CCRYGG 1 cut(s) 365
BstEII GGTNACC 1 cut(s) 1151
BstENI CCTNNNNNAGG 1 cut(s) 1206
BstF5I GGATG 3 cut(s) 139, 249, 1342
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 2 cut(s) 375, 1300
BstMAI GTCTC 3 cut(s) 62, 244, 1321
BstNI CCWGG 2 cut(s) 399, 1323
BstNSI RCATGY 1 cut(s) 949
BstPI GGTNACC 1 cut(s) 1151
BstSCI CCNGG 3 cut(s) 397, 775, 1321
BstV1I GCAGC 2 cut(s) 920, 1282
BstV2I GAAGAC 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 1306
Bsu36I CCTNAGG 2 cut(s) 996, 1251
BsuI GTATCC 1 cut(s) 1194
BsuRI GGCC 1 cut(s) 819
BsuTUI ATCGAT 1 cut(s) 1306
BtgI CCRYGG 1 cut(s) 365
BtsCI GGATG 3 cut(s) 139, 249, 1342
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
CfoI GCGC 2 cut(s) 375, 1300
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 1 cut(s) 779
ClaI ATCGAT 1 cut(s) 1306
CseI GACGC 1 cut(s) 263
CspAI ACCGGT 1 cut(s) 157
CviAII CATG 2 cut(s) 491, 946
DdeI CTNAG 6 cut(s) 270, 393, 858, 996, 1053, 1251
EaeI YGGCCR 1 cut(s) 817
EciI GGCGGA 3 cut(s) 122, 580, 658
Eco130I CCWWGG 4 cut(s) 554, 710, 752, 1207
Eco24I GRGCYC 1 cut(s) 271
Eco32I GATATC 1 cut(s) 826
Eco47I GGWCC 1 cut(s) 779
Eco47III AGCGCT 1 cut(s) 374
Eco57I CTGAAG 3 cut(s) 315, 1068, 1209
Eco81I CCTNAGG 2 cut(s) 996, 1251
Eco91I GGTNACC 1 cut(s) 1151
EcoNI CCTNNNNNAGG 1 cut(s) 1206
EcoO109I RGGNCCY 1 cut(s) 779
EcoO65I GGTNACC 1 cut(s) 1151
EcoRII CCWGG 2 cut(s) 397, 1321
EcoRV GATATC 1 cut(s) 826
EcoT14I CCWWGG 4 cut(s) 554, 710, 752, 1207
EcoT38I GRGCYC 1 cut(s) 271
ErhI CCWWGG 4 cut(s) 554, 710, 752, 1207
Esp3I CGTCTC 1 cut(s) 62
FaeI CATG 2 cut(s) 494, 949
FaiI YATR 8 cut(s) 282, 290, 492, 947, 1269, 1311, 1316, 1439
FaqI GGGAC 3 cut(s) 261, 305, 740
FatI CATG 2 cut(s) 490, 945
FauI CCCGC 1 cut(s) 1032
Fnu4HI GCNGC 4 cut(s) 6, 431, 909, 1296
FokI GGATG 3 cut(s) 146, 256, 1349
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 4 cut(s) 6, 431, 909, 1296
FspBI CTAG 5 cut(s) 51, 1058, 1101, 1208, 1263
GlaI GCGC 2 cut(s) 374, 1299
GluI GCNGC 4 cut(s) 6, 431, 909, 1296
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 1 cut(s) 819
HapII CCGG 2 cut(s) 158, 776
HgaI GACGC 1 cut(s) 263
HhaI GCGC 2 cut(s) 375, 1300
Hin1II CATG 2 cut(s) 494, 949
Hin6I GCGC 2 cut(s) 373, 1298
HinP1I GCGC 2 cut(s) 373, 1298
HinfI GANTC 2 cut(s) 47, 1232
HpaII CCGG 2 cut(s) 158, 776
HphI GGTGA 6 cut(s) 172, 247, 351, 381, 545, 1163
Hpy188I TCNGA 7 cut(s) 295, 327, 343, 421, 892, 952, 973
Hpy188III TCNNGA 5 cut(s) 867, 1112, 1187, 1236, 1253
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 5 cut(s) 329, 897, 1072, 1126, 1222
HpyCH4IV ACGT 1 cut(s) 1072
HpyCH4V TGCA 3 cut(s) 617, 695, 1094
HpyF3I CTNAG 6 cut(s) 270, 393, 858, 996, 1053, 1251
HpySE526I ACGT 1 cut(s) 1072
Hsp92II CATG 2 cut(s) 494, 949
HspAI GCGC 2 cut(s) 373, 1298
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 2 cut(s) 344, 958
Lsp1109I GCAGC 2 cut(s) 920, 1282
LweI GCATC 2 cut(s) 274, 1282
MaeI CTAG 5 cut(s) 51, 1058, 1101, 1208, 1263
MaeII ACGT 1 cut(s) 1072
MaeIII GTNAC 3 cut(s) 357, 1151, 1352
MhlI GDGCHC 1 cut(s) 271
MlsI TGGCCA 1 cut(s) 819
MluCI AATT 3 cut(s) 165, 187, 1064
MluNI TGGCCA 1 cut(s) 819
MmeI TCCRAC 2 cut(s) 930, 990
Mox20I TGGCCA 1 cut(s) 819
MroXI GAANNNNTTC 2 cut(s) 406, 1194
MscI TGGCCA 1 cut(s) 819
MseI TTAA 5 cut(s) 24, 123, 263, 315, 1277
MslI CAYNNNNRTG 2 cut(s) 233, 1335
Msp20I TGGCCA 1 cut(s) 819
MspI CCGG 2 cut(s) 158, 776
MspR9I CCNGG 3 cut(s) 399, 777, 1323
MvaI CCWGG 2 cut(s) 399, 1323
NciI CCSGG 1 cut(s) 777
NlaIII CATG 2 cut(s) 494, 949
NlaIV GGNNCC 4 cut(s) 340, 488, 780, 885
NmeAIII GCCGAG 1 cut(s) 459
NmuCI GTSAC 2 cut(s) 357, 1151
NspI RCATGY 1 cut(s) 949
PciSI GCTCTTC 1 cut(s) 92
PdmI GAANNNNTTC 2 cut(s) 406, 1194
PfeI GAWTC 2 cut(s) 47, 1232
PflMI CCANNNNNTGG 1 cut(s) 803
PinAI ACCGGT 1 cut(s) 157
PkrI GCNGC 4 cut(s) 7, 432, 910, 1297
PpuMI RGGWCCY 1 cut(s) 779
Psp5II RGGWCCY 1 cut(s) 779
Psp6I CCWGG 2 cut(s) 397, 1321
PspEI GGTNACC 1 cut(s) 1151
PspGI CCWGG 2 cut(s) 397, 1321
PspN4I GGNNCC 4 cut(s) 340, 488, 780, 885
PspPI GGNCC 1 cut(s) 779
PspPPI RGGWCCY 1 cut(s) 779
RseI CAYNNNNRTG 2 cut(s) 233, 1335
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 5 cut(s) 24, 123, 263, 315, 1277
SatI GCNGC 4 cut(s) 6, 431, 909, 1296
Sau96I GGNCC 1 cut(s) 779
ScrFI CCNGG 3 cut(s) 399, 777, 1323
SduI GDGCHC 1 cut(s) 271
SfaNI GCATC 2 cut(s) 274, 1282
SinI GGWCC 1 cut(s) 779
SmiMI CAYNNNNRTG 2 cut(s) 233, 1335
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 3 cut(s) 165, 187, 1064
SsiI CCGC 7 cut(s) 5, 107, 431, 497, 565, 643, 1039
SspMI CTAG 5 cut(s) 51, 1058, 1101, 1208, 1263
StyD4I CCNGG 3 cut(s) 397, 775, 1321
StyI CCWWGG 4 cut(s) 554, 710, 752, 1207
TaiI ACGT 1 cut(s) 1075
TaqI TCGA 3 cut(s) 410, 866, 1306
TasI AATT 3 cut(s) 165, 187, 1064
TauI GCSGC 2 cut(s) 8, 433
TfiI GAWTC 2 cut(s) 47, 1232
Tru1I TTAA 5 cut(s) 24, 123, 263, 315, 1277
Tru9I TTAA 5 cut(s) 24, 123, 263, 315, 1277
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 2 cut(s) 357, 1151
TseI GCWGC 2 cut(s) 908, 1295
Tsp45I GTSAC 2 cut(s) 357, 1151
TspDTI ATGAA 7 cut(s) 150, 237, 260, 750, 1002, 1145, 1241
TspGWI ACGGA 5 cut(s) 382, 1111, 1371, 1396, 1424
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 803
VpaK11BI GGWCC 1 cut(s) 779
XagI CCTNNNNNAGG 1 cut(s) 1206
XceI RCATGY 1 cut(s) 949
XcmI CCANNNNNNNNNTGG 1 cut(s) 1329
XmaJI CCTAGG 1 cut(s) 1207
XmnI GAANNNNTTC 2 cut(s) 406, 1194
XspI CTAG 5 cut(s) 51, 1058, 1101, 1208, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.