Rw4G036870

BUD13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010092
Physical Location & Seq
Reverse (-)
63684641 .. 63688525
3885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G036870.1

Sequence Viewer

Length: 1530 bp
ATGGCGGCTTCCACGAAATCGCTTAAAGAGTATTTGAAACGATACGAATCTAGTAATAACGAGGACGATACGAAGAAGAAGAGGAAGAAGAAGCAGAAGAGCAAGGCGGAGGCAATGGGGGTTAAGGTGGTGGATGAGGACCCTGTGTGGCAGAAACCGGTGAAAATTGAGGGAGAAGAAGATAACAATTCAGCTGACGAAGAGGAGAAGCCTTTAGTTGATGAAGACATTGAGGTGAAGAGGATGAAGAGGCTTGAGTTATTAAGGGCGCGGCGGCCCTATGATGCTATATCTGAAGATGGCAGTGGTTGGGTTAAGGTCCCTCTGAAACGAGAAGGCTCCGAAGAGGCTGACTTGTCACCTCCGCGGAAGCGGTATCTTTCTCCCTCACCTGAGCCTGGAATGGCTTCGAGGGGATCAGACCTTTCGCCGCCGAGGAAAAGTAGGAGGGGCGGTGATGAAGATCTTTCTCCACCGAGGAAGAGTAGGAGCAATGTTGAAGATCTTTCTCCACCGAGGAAGAGTAGGAACAATTTTGAAGATCTTTCTCCACCGAGGAAGGGTAGGAACAATGGTGATGGAGATCTTTCTCCACCAAGGAAGAGGAGGAACAATGTTGCAGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGGTGATGGAGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGTTGTAGATCTTTCTCCACCAAGGAACAGGCGGAACAATGTTGAAGATCTTTCTCCACCAAGGAAGAGAAGGAACAATGGTGATGCAGATCTTTCTCCACCAAGGAAAAGTAGGAGGGATATTGATGAAGATCTATCTCCACCAAGGAAAAAGAAGCAAGTATCTCCGGGTCCTTTGAAAGAGAAGCCAAAAAGTGGTTTGATTTCTGGCCGAGATATCAGCGAAGAGATTTCTAAAACGAAGAGAGCTGAGCAGTCGAGATTTGAACAGATGGATCCTTCTGAAACTGGGCAACAAGCAGCAGTTGTATTTCGTAATAAGAAAACAGGAGAACGCATGTCGGAGGAGGAGCGATTGAAATCAGAAAAGAAACTTGATGAAAAGCCTAAGGAGATAAAGTTGGAATGGGGTAAGGGCTTGGCTCAAAAGCGGGAAGCTGAAGCTAAGCTAGAGGAATTAGAACGTGAGAAGGAAAAACCATTTGCACGGACTAGAGATGATCCAGAACTTGACAAAATGATGAAGGAGAGAGTAAGATGGGGTGACCCAATGGCACATTTGGTAAAGAAAAAGTATCCTGAAGCAGTTCTCCCTAACCTAGGGGATAGCGAGAAAATGAAGGACTCTGGATTTATTATCCCTCAGGATATTCCTAATCATAGCTGGTTAAAAAGAGGATTAGATGCTGCACCAAATCGATATGGTATAAAACCAGGGAGACATTGGGATGGAGTTGATCGCAGTAACGGATTTGAGAAAGGATTGTTCAAACGGACTAACGAGAAACAAGCTACTCAGACGGAGGCTTATCTTTGGTCTGTCGCAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bud13

Protein Analysis

509

Amino Acids

58.19

Weight (kDa)

9.63

Isoelectric Point (pI)

66.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bud13 PF09736 334 - 492 6.2e-40 Pre-mRNA-splicing factor of RES complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 890
AccII CGCG 2 cut(s) 271, 367
AclWI GGATC 4 cut(s) 424, 965, 978, 1190
AcoI YGGCCR 1 cut(s) 904
AcuI CTGAAG 3 cut(s) 315, 1155, 1296
AfiI CCNNNNNNNGG 4 cut(s) 398, 560, 890, 1295
AgeI ACCGGT 1 cut(s) 157
AgsI TTSAA 8 cut(s) 37, 500, 539, 740, 874, 962, 1054, 1465
AjnI CCWGG 2 cut(s) 397, 1408
AluBI AGCT 7 cut(s) 194, 944, 1133, 1139, 1144, 1359, 1487
AluI AGCT 7 cut(s) 194, 944, 1133, 1139, 1144, 1359, 1487
Alw26I GTCTC 1 cut(s) 1408
AlwI GGATC 4 cut(s) 424, 965, 978, 1190
AoxI GGCC 2 cut(s) 275, 904
ApeKI GCWGC 2 cut(s) 995, 1382
AsiGI ACCGGT 1 cut(s) 157
Asp700I GAANNNNTTC 2 cut(s) 406, 1281
AspA2I CCTAGG 1 cut(s) 1294
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 4 cut(s) 139, 276, 319, 866
AsuC2I CCSGG 1 cut(s) 864
AsuHPI GGTGA 9 cut(s) 172, 247, 351, 381, 467, 587, 668, 788, 1250
AvaII GGWCC 3 cut(s) 139, 319, 866
AvrII CCTAGG 1 cut(s) 1294
AxyI CCTNAGG 2 cut(s) 1083, 1338
BamHI GGATCC 1 cut(s) 970
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 1007, 1369
BccI CCATC 6 cut(s) 293, 572, 653, 961, 1227, 1418
BciT130I CCWGG 2 cut(s) 399, 1410
BciVI GTATCC 1 cut(s) 1281
BcnI CCSGG 1 cut(s) 864
BcoDI GTCTC 1 cut(s) 1408
BfaI CTAG 4 cut(s) 51, 1145, 1188, 1295
BfuI GTATCC 1 cut(s) 1281
BisI GCNGC 6 cut(s) 6, 272, 275, 431, 996, 1383
BlnI CCTAGG 1 cut(s) 1294
BlpI GCTNAGC 2 cut(s) 945, 1140
BlsI GCNGC 6 cut(s) 7, 273, 276, 432, 997, 1384
Bme1390I CCNGG 3 cut(s) 399, 864, 1410
Bme18I GGWCC 3 cut(s) 139, 319, 866
BmgT120I GGNCC 4 cut(s) 139, 276, 319, 866
BmiI GGNNCC 5 cut(s) 141, 321, 340, 867, 972
BmrFI CCNGG 3 cut(s) 399, 864, 1410
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 3 cut(s) 274, 769, 1369
BmuI ACTGGG 1 cut(s) 993
BpiI GAAGAC 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 393
Bpu1102I GCTNAGC 2 cut(s) 945, 1140
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 864
Bsa29I ATCGAT 1 cut(s) 1393
BsaBI GATNNNNATC 7 cut(s) 46, 462, 582, 663, 783, 825, 1054
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 4 cut(s) 439, 469, 1079, 1109
Bsc4I CCNNNNNNNGG 4 cut(s) 398, 560, 890, 1295
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 988
Bse21I CCTNAGG 2 cut(s) 1083, 1338
Bse3DI GCAATG 2 cut(s) 120, 499
Bse8I GATNNNNATC 7 cut(s) 46, 462, 582, 663, 783, 825, 1054
BseBI CCWGG 2 cut(s) 399, 1410
BseCI ATCGAT 1 cut(s) 1393
BseGI GGATG 3 cut(s) 139, 249, 1429
BseJI GATNNNNATC 7 cut(s) 46, 462, 582, 663, 783, 825, 1054
BseLI CCNNNNNNNGG 4 cut(s) 398, 560, 890, 1295
BseMI GCAATG 2 cut(s) 120, 499
BseMII CTCAG 4 cut(s) 384, 936, 1352, 1505
BseNI ACTGG 1 cut(s) 988
BseRI GAGGAG 4 cut(s) 218, 619, 1055, 1058
BseXI GCAGC 2 cut(s) 1007, 1369
BsgI GTGCAG 1 cut(s) 1368
Bsh1236I CGCG 2 cut(s) 271, 367
BshFI GGCC 2 cut(s) 277, 906
BshTI ACCGGT 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 1393
BsiSI CCGG 2 cut(s) 158, 863
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 4 cut(s) 398, 560, 890, 1295
BsmAI GTCTC 1 cut(s) 1408
BsmFI GGGAC 1 cut(s) 305
BsnI GGCC 2 cut(s) 277, 906
Bsp1720I GCTNAGC 2 cut(s) 945, 1140
BspANI GGCC 2 cut(s) 277, 906
BspCNI CTCAG 4 cut(s) 385, 937, 1351, 1504
BspDI ATCGAT 1 cut(s) 1393
BspFNI CGCG 2 cut(s) 271, 367
BspLI GGNNCC 5 cut(s) 141, 321, 340, 867, 972
BspPI GGATC 4 cut(s) 424, 965, 978, 1190
BspQI GCTCTTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 120, 499
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 988
BssAI RCCGGY 1 cut(s) 157
BssT1I CCWWGG 6 cut(s) 596, 716, 755, 797, 839, 1294
Bst2UI CCWGG 2 cut(s) 399, 1410
BstDEI CTNAG 6 cut(s) 393, 945, 1083, 1140, 1338, 1491
BstDSI CCRYGG 1 cut(s) 365
BstEII GGTNACC 1 cut(s) 1238
BstENI CCTNNNNNAGG 1 cut(s) 1293
BstF5I GGATG 3 cut(s) 139, 249, 1429
BstFNI CGCG 2 cut(s) 271, 367
BstHHI GCGC 1 cut(s) 271
BstMAI GTCTC 1 cut(s) 1408
BstNI CCWGG 2 cut(s) 399, 1410
BstNSI RCATGY 1 cut(s) 1036
BstPI GGTNACC 1 cut(s) 1238
BstSCI CCNGG 3 cut(s) 397, 862, 1408
BstUI CGCG 2 cut(s) 271, 367
BstV1I GCAGC 2 cut(s) 1007, 1369
BstV2I GAAGAC 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 1393
Bsu36I CCTNAGG 2 cut(s) 1083, 1338
BsuI GTATCC 1 cut(s) 1281
BsuRI GGCC 2 cut(s) 277, 906
BsuTUI ATCGAT 1 cut(s) 1393
BtgI CCRYGG 1 cut(s) 365
BtsCI GGATG 3 cut(s) 139, 249, 1429
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
CfoI GCGC 1 cut(s) 271
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 4 cut(s) 139, 276, 319, 866
Cfr42I CCGCGG 1 cut(s) 368
ClaI ATCGAT 1 cut(s) 1393
CspAI ACCGGT 1 cut(s) 157
CviAII CATG 1 cut(s) 1033
DdeI CTNAG 6 cut(s) 393, 945, 1083, 1140, 1338, 1491
EaeI YGGCCR 1 cut(s) 904
EciI GGCGGA 2 cut(s) 122, 742
Eco130I CCWWGG 6 cut(s) 596, 716, 755, 797, 839, 1294
Eco32I GATATC 1 cut(s) 913
Eco47I GGWCC 3 cut(s) 139, 319, 866
Eco57I CTGAAG 3 cut(s) 315, 1155, 1296
Eco81I CCTNAGG 2 cut(s) 1083, 1338
Eco91I GGTNACC 1 cut(s) 1238
EcoNI CCTNNNNNAGG 1 cut(s) 1293
EcoO109I RGGNCCY 3 cut(s) 139, 319, 866
EcoO65I GGTNACC 1 cut(s) 1238
EcoRII CCWGG 2 cut(s) 397, 1408
EcoRV GATATC 1 cut(s) 913
EcoT14I CCWWGG 6 cut(s) 596, 716, 755, 797, 839, 1294
ErhI CCWWGG 6 cut(s) 596, 716, 755, 797, 839, 1294
FaeI CATG 1 cut(s) 1036
FaiI YATR 7 cut(s) 282, 290, 1034, 1356, 1398, 1403, 1526
FaqI GGGAC 1 cut(s) 305
FatI CATG 1 cut(s) 1032
FauI CCCGC 1 cut(s) 1119
Fnu4HI GCNGC 6 cut(s) 6, 272, 275, 431, 996, 1383
FokI GGATG 3 cut(s) 146, 256, 1436
Fsp4HI GCNGC 6 cut(s) 6, 272, 275, 431, 996, 1383
FspBI CTAG 4 cut(s) 51, 1145, 1188, 1295
GlaI GCGC 1 cut(s) 270
GluI GCNGC 6 cut(s) 6, 272, 275, 431, 996, 1383
HaeIII GGCC 2 cut(s) 277, 906
HapII CCGG 2 cut(s) 158, 863
HhaI GCGC 1 cut(s) 271
Hin1II CATG 1 cut(s) 1036
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HinfI GANTC 2 cut(s) 47, 1319
HpaII CCGG 2 cut(s) 158, 863
HphI GGTGA 9 cut(s) 172, 247, 351, 381, 467, 587, 668, 788, 1250
Hpy188I TCNGA 8 cut(s) 295, 327, 343, 421, 979, 1039, 1060, 1494
Hpy188III TCNNGA 5 cut(s) 954, 1199, 1274, 1323, 1340
HpyAV CCTTC 7 cut(s) 329, 553, 759, 984, 1159, 1213, 1309
HpyCH4IV ACGT 1 cut(s) 1159
HpyCH4V TGCA 4 cut(s) 620, 782, 1181, 1385
HpyF3I CTNAG 6 cut(s) 393, 945, 1083, 1140, 1338, 1491
HpySE526I ACGT 1 cut(s) 1159
Hsp92II CATG 1 cut(s) 1036
HspAI GCGC 1 cut(s) 269
KspI CCGCGG 1 cut(s) 368
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 3 cut(s) 344, 489, 1045
Lsp1109I GCAGC 2 cut(s) 1007, 1369
LweI GCATC 3 cut(s) 274, 769, 1369
MaeI CTAG 4 cut(s) 51, 1145, 1188, 1295
MaeII ACGT 1 cut(s) 1159
MaeIII GTNAC 3 cut(s) 357, 1238, 1439
MluCI AATT 4 cut(s) 165, 187, 532, 1151
MlyI GAGTC 1 cut(s) 1313
MmeI TCCRAC 2 cut(s) 1017, 1077
MroXI GAANNNNTTC 2 cut(s) 406, 1281
MseI TTAA 5 cut(s) 24, 123, 263, 315, 1364
MslI CAYNNNNRTG 2 cut(s) 233, 1422
MspA1I CMGCKG 2 cut(s) 194, 367
MspI CCGG 2 cut(s) 158, 863
MspR9I CCNGG 3 cut(s) 399, 864, 1410
MvaI CCWGG 2 cut(s) 399, 1410
MvnI CGCG 2 cut(s) 271, 367
NciI CCSGG 1 cut(s) 864
NlaIII CATG 1 cut(s) 1036
NlaIV GGNNCC 5 cut(s) 141, 321, 340, 867, 972
NmeAIII GCCGAG 2 cut(s) 459, 932
NmuCI GTSAC 2 cut(s) 357, 1238
NspI RCATGY 1 cut(s) 1036
PciSI GCTCTTC 1 cut(s) 92
PdmI GAANNNNTTC 2 cut(s) 406, 1281
PfeI GAWTC 1 cut(s) 47
PflMI CCANNNNNTGG 1 cut(s) 890
PinAI ACCGGT 1 cut(s) 157
PkrI GCNGC 6 cut(s) 7, 273, 276, 432, 997, 1384
PleI GAGTC 1 cut(s) 1313
PpsI GAGTC 1 cut(s) 1313
PpuMI RGGWCCY 3 cut(s) 139, 319, 866
Psp5II RGGWCCY 3 cut(s) 139, 319, 866
Psp6I CCWGG 2 cut(s) 397, 1408
PspEI GGTNACC 1 cut(s) 1238
PspGI CCWGG 2 cut(s) 397, 1408
PspN4I GGNNCC 5 cut(s) 141, 321, 340, 867, 972
PspPI GGNCC 4 cut(s) 139, 276, 319, 866
PspPPI RGGWCCY 3 cut(s) 139, 319, 866
PvuII CAGCTG 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 233, 1422
SacII CCGCGG 1 cut(s) 368
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 5 cut(s) 24, 123, 263, 315, 1364
SatI GCNGC 6 cut(s) 6, 272, 275, 431, 996, 1383
Sau96I GGNCC 4 cut(s) 139, 276, 319, 866
SchI GAGTC 1 cut(s) 1313
ScrFI CCNGG 3 cut(s) 399, 864, 1410
SfaNI GCATC 3 cut(s) 274, 769, 1369
Sfr303I CCGCGG 1 cut(s) 368
SgrBI CCGCGG 1 cut(s) 368
SinI GGWCC 3 cut(s) 139, 319, 866
SmiMI CAYNNNNRTG 2 cut(s) 233, 1422
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 4 cut(s) 165, 187, 532, 1151
SspMI CTAG 4 cut(s) 51, 1145, 1188, 1295
StyD4I CCNGG 3 cut(s) 397, 862, 1408
StyI CCWWGG 6 cut(s) 596, 716, 755, 797, 839, 1294
TaiI ACGT 1 cut(s) 1162
TaqI TCGA 3 cut(s) 410, 953, 1393
TasI AATT 4 cut(s) 165, 187, 532, 1151
TauI GCSGC 4 cut(s) 8, 274, 277, 433
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 5 cut(s) 24, 123, 263, 315, 1364
Tru9I TTAA 5 cut(s) 24, 123, 263, 315, 1364
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 2 cut(s) 357, 1238
TseI GCWGC 2 cut(s) 995, 1382
Tsp45I GTSAC 2 cut(s) 357, 1238
TspDTI ATGAA 7 cut(s) 237, 260, 474, 837, 1089, 1232, 1328
TspGWI ACGGA 4 cut(s) 1198, 1458, 1483, 1511
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 890
VpaK11BI GGWCC 3 cut(s) 139, 319, 866
XagI CCTNNNNNAGG 1 cut(s) 1293
XceI RCATGY 1 cut(s) 1036
XcmI CCANNNNNNNNNTGG 1 cut(s) 1416
XmaJI CCTAGG 1 cut(s) 1294
XmnI GAANNNNTTC 2 cut(s) 406, 1281
XspI CTAG 4 cut(s) 51, 1145, 1188, 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.