Rh4CG458400

BUD13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0010092
Physical Location & Seq
Reverse (-)
68982081 .. 68985309
3229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG458400.1

Sequence Viewer

Length: 1449 bp
ATGGCGGCTTCCACGAAATCGCTTAAAGAGTATTTGAAACGATACGAATCTAGTAATAACGAGGACGAGACGAAGAAGAAGAGGAAGAAGAAGCAGAAGAGCAAGGCGGAGGCAATGGGGGTTAAAGTGGTAGATGAAGACCCTGTGTGGCAGAAACCGGTGAAAGCTGAGAGAGAAGATGATAACAATTCAGCTGACGAAGAGGAGAAGCCTTTAGTTGATGAAGACATTGAGGTGAAGAGGATGAAGAGACTTGAGTTATTAAGGGCTCGGCGTCCCTATGATGCTATATCTGAAGATGGCAGTGGTTGGGTTAAGGTCCCTCTGAAACGAGAAGGCTCCGAAGAGGCTGACTTGTCACCTCCGCGGAAGCGCTATCATTCTCCCTCACCTGAGCCTGGAATGACTTCGAGGGGATCAGACCTTTCGCCGCCGAGGAAAAGTAGGAGGAGCAATGTTGAAGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGTTGAAGATCTTTCTCCACCGAGGAAGAGGAGGAACAATGTTGCGGATCTTTCACCACCAAGGAAGAGTAGGAACAATGGTGATGGAGATCTTTCTCCACCAAGGAACAGGCGGAACAATGTTGCAGATCTTTCTCCACCGAGGAACAGGCGGAACAATGTTGAAGATCTTTCTCCACCAAGGAAGAGAAGGAACAATGGTGATGCAGATCTTTCTCCACCAAGAAAAAGTAGGAGGGATATTGATGAAGATCTATCTCCACCAAGGAAAAAGAAGCAAGTATCTCCCGGTCCTTTGAAAGAGAAGCCAAAAAGTGGTTTGATATCTGGCCGAGATATCAGCGAAGAGATTTCTAAAACGAAGAGAGCTGAGCAGTCGAGATTTGAACAGATGGATCCTTCTGAAACTGGGCAACAAGCAGCAGTTGTATTTCGTAATAAGAAAACAGGAGAACGCATGTCGGAGGAGGAGCGATTGAAATCAGAAAAGAAACTTGATGAAAAGCCTAAGGAGATAAAGTTGGAATGGGGTAAGGGCTTGGCTCAAAAGCGGGAAGCTGAAGCTAAGCTAGAGGAAATAGAACGTGAGAAGGAAAAACCATTTGCACGGACTAGAGATGATCCAGAACTTGACAAAATGATGAAGGAGAGAGTAAGATGGGGTGACCCAATGGCACATTTGGTAAAGAAAAAGTATCCTGAAGCAGTTCTCCCTAACCTAGGGGATAGCGAGCAAATGAAGGAATCTGGATTTATTATCCCTCAGGATATTCCTAATCATAGCTGGTTAAAAAGAGGATTAGATGCTGCACCAAATCGATATGGTATAAAACCAGGGAGACATTGGGATGGAGTTGATCGCAGTAACGGATTTGAGAAAGGATTGTTCAAACGGACTAACGAGAAACAAGCTACTCAGACGGAGGCTTATCTTTGGTCTGTCGCAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bud13

Protein Analysis

482

Amino Acids

55.44

Weight (kDa)

9.64

Isoelectric Point (pI)

68.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bud13 PF09736 307 - 465 9.7e-40 Pre-mRNA-splicing factor of RES complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 809
AccII CGCG 1 cut(s) 367
AciI CCGC 9 cut(s) 5, 107, 365, 367, 431, 539, 607, 646, 1045
AclWI GGATC 5 cut(s) 424, 549, 884, 897, 1109
AcoI YGGCCR 1 cut(s) 823
AcuI CTGAAG 3 cut(s) 315, 1074, 1215
AcyI GRCGYC 1 cut(s) 274
AfeI AGCGCT 1 cut(s) 374
AfiI CCNNNNNNNGG 3 cut(s) 398, 809, 1214
AgeI ACCGGT 1 cut(s) 157
AgsI TTSAA 8 cut(s) 37, 461, 500, 659, 793, 881, 973, 1384
AjnI CCWGG 2 cut(s) 397, 1327
AluBI AGCT 8 cut(s) 167, 194, 863, 1052, 1058, 1063, 1278, 1406
AluI AGCT 8 cut(s) 167, 194, 863, 1052, 1058, 1063, 1278, 1406
Alw26I GTCTC 3 cut(s) 62, 244, 1327
AlwI GGATC 5 cut(s) 424, 549, 884, 897, 1109
Aor51HI AGCGCT 1 cut(s) 374
AoxI GGCC 1 cut(s) 823
ApeKI GCWGC 2 cut(s) 914, 1301
AsiGI ACCGGT 1 cut(s) 157
Asp700I GAANNNNTTC 2 cut(s) 406, 1200
AspA2I CCTAGG 1 cut(s) 1213
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 2 cut(s) 319, 785
AsuC2I CCSGG 1 cut(s) 783
AsuHPI GGTGA 8 cut(s) 172, 247, 351, 381, 540, 587, 707, 1169
AvaII GGWCC 2 cut(s) 319, 785
AvrII CCTAGG 1 cut(s) 1213
AxyI CCTNAGG 2 cut(s) 1002, 1257
BamHI GGATCC 1 cut(s) 889
BanII GRGCYC 1 cut(s) 271
BbsI GAAGAC 2 cut(s) 144, 231
BbvI GCAGC 2 cut(s) 926, 1288
BccI CCATC 5 cut(s) 293, 572, 880, 1146, 1337
BciT130I CCWGG 2 cut(s) 399, 1329
BciVI GTATCC 1 cut(s) 1200
BcnI CCSGG 1 cut(s) 783
BcoDI GTCTC 3 cut(s) 62, 244, 1327
BfaI CTAG 4 cut(s) 51, 1064, 1107, 1214
BfoI RGCGCY 1 cut(s) 376
BfuI GTATCC 1 cut(s) 1200
BglII AGATCT 7 cut(s) 463, 502, 583, 622, 661, 703, 745
BisI GCNGC 4 cut(s) 6, 431, 915, 1302
BlnI CCTAGG 1 cut(s) 1213
BlpI GCTNAGC 2 cut(s) 864, 1059
BlsI GCNGC 4 cut(s) 7, 432, 916, 1303
Bme1390I CCNGG 3 cut(s) 399, 783, 1329
Bme18I GGWCC 2 cut(s) 319, 785
BmgT120I GGNCC 2 cut(s) 319, 785
BmiI GGNNCC 3 cut(s) 321, 340, 891
BmrFI CCNGG 3 cut(s) 399, 783, 1329
BmrI ACTGGG 1 cut(s) 912
BmsI GCATC 3 cut(s) 274, 688, 1288
BmuI ACTGGG 1 cut(s) 912
BpiI GAAGAC 2 cut(s) 144, 231
Bpu10I CCTNAGC 1 cut(s) 393
Bpu1102I GCTNAGC 2 cut(s) 864, 1059
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 783
Bsa29I ATCGAT 1 cut(s) 1312
BsaBI GATNNNNATC 5 cut(s) 46, 582, 702, 744, 973
BsaHI GRCGYC 1 cut(s) 274
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 998, 1028
Bsc4I CCNNNNNNNGG 3 cut(s) 398, 809, 1214
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 907
Bse21I CCTNAGG 2 cut(s) 1002, 1257
Bse3DI GCAATG 2 cut(s) 120, 460
Bse8I GATNNNNATC 5 cut(s) 46, 582, 702, 744, 973
BseBI CCWGG 2 cut(s) 399, 1329
BseCI ATCGAT 1 cut(s) 1312
BseGI GGATG 2 cut(s) 249, 1348
BseJI GATNNNNATC 5 cut(s) 46, 582, 702, 744, 973
BseLI CCNNNNNNNGG 3 cut(s) 398, 809, 1214
BseMI GCAATG 2 cut(s) 120, 460
BseMII CTCAG 5 cut(s) 159, 384, 855, 1271, 1424
BseNI ACTGG 1 cut(s) 907
BseRI GAGGAG 5 cut(s) 218, 463, 538, 974, 977
BseXI GCAGC 2 cut(s) 926, 1288
BsgI GTGCAG 1 cut(s) 1287
Bsh1236I CGCG 1 cut(s) 367
BshFI GGCC 1 cut(s) 825
BshTI ACCGGT 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 1312
BsiSI CCGG 2 cut(s) 158, 783
BslFI GGGAC 2 cut(s) 261, 305
BslI CCNNNNNNNGG 3 cut(s) 398, 809, 1214
BsmAI GTCTC 3 cut(s) 62, 244, 1327
BsmBI CGTCTC 1 cut(s) 62
BsmFI GGGAC 2 cut(s) 261, 305
BsnI GGCC 1 cut(s) 825
Bsp1286I GDGCHC 1 cut(s) 271
Bsp1720I GCTNAGC 2 cut(s) 864, 1059
BspACI CCGC 9 cut(s) 5, 107, 365, 367, 431, 539, 607, 646, 1045
BspANI GGCC 1 cut(s) 825
BspCNI CTCAG 5 cut(s) 160, 385, 856, 1270, 1423
BspDI ATCGAT 1 cut(s) 1312
BspFNI CGCG 1 cut(s) 367
BspLI GGNNCC 3 cut(s) 321, 340, 891
BspPI GGATC 5 cut(s) 424, 549, 884, 897, 1109
BspQI GCTCTTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 120, 460
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 907
BssAI RCCGGY 1 cut(s) 157
BssNI GRCGYC 1 cut(s) 274
BssT1I CCWWGG 5 cut(s) 554, 596, 674, 758, 1213
Bst2UI CCWGG 2 cut(s) 399, 1329
BstACI GRCGYC 1 cut(s) 274
BstC8I GCNNGC 1 cut(s) 1226
BstDEI CTNAG 7 cut(s) 168, 393, 864, 1002, 1059, 1257, 1410
BstDSI CCRYGG 1 cut(s) 365
BstEII GGTNACC 1 cut(s) 1157
BstENI CCTNNNNNAGG 1 cut(s) 1212
BstF5I GGATG 2 cut(s) 249, 1348
BstFNI CGCG 1 cut(s) 367
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 1 cut(s) 375
BstMAI GTCTC 3 cut(s) 62, 244, 1327
BstNI CCWGG 2 cut(s) 399, 1329
BstNSI RCATGY 1 cut(s) 955
BstPI GGTNACC 1 cut(s) 1157
BstSCI CCNGG 3 cut(s) 397, 781, 1327
BstUI CGCG 1 cut(s) 367
BstV1I GCAGC 2 cut(s) 926, 1288
BstV2I GAAGAC 2 cut(s) 144, 231
BstX2I RGATCY 9 cut(s) 463, 502, 541, 583, 622, 661, 703, 745, 889
BstYI RGATCY 9 cut(s) 463, 502, 541, 583, 622, 661, 703, 745, 889
Bsu15I ATCGAT 1 cut(s) 1312
Bsu36I CCTNAGG 2 cut(s) 1002, 1257
BsuI GTATCC 1 cut(s) 1200
BsuRI GGCC 1 cut(s) 825
BsuTUI ATCGAT 1 cut(s) 1312
BtgI CCRYGG 1 cut(s) 365
BtsCI GGATG 2 cut(s) 249, 1348
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 1226
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 319, 785
Cfr42I CCGCGG 1 cut(s) 368
ClaI ATCGAT 1 cut(s) 1312
CseI GACGC 1 cut(s) 263
CspAI ACCGGT 1 cut(s) 157
CviAII CATG 1 cut(s) 952
DdeI CTNAG 7 cut(s) 168, 393, 864, 1002, 1059, 1257, 1410
EaeI YGGCCR 1 cut(s) 823
EciI GGCGGA 3 cut(s) 122, 622, 661
Eco130I CCWWGG 5 cut(s) 554, 596, 674, 758, 1213
Eco24I GRGCYC 1 cut(s) 271
Eco32I GATATC 2 cut(s) 819, 832
Eco47I GGWCC 2 cut(s) 319, 785
Eco47III AGCGCT 1 cut(s) 374
Eco57I CTGAAG 3 cut(s) 315, 1074, 1215
Eco81I CCTNAGG 2 cut(s) 1002, 1257
Eco91I GGTNACC 1 cut(s) 1157
EcoNI CCTNNNNNAGG 1 cut(s) 1212
EcoO109I RGGNCCY 1 cut(s) 319
EcoO65I GGTNACC 1 cut(s) 1157
EcoRII CCWGG 2 cut(s) 397, 1327
EcoRV GATATC 2 cut(s) 819, 832
EcoT14I CCWWGG 5 cut(s) 554, 596, 674, 758, 1213
EcoT38I GRGCYC 1 cut(s) 271
ErhI CCWWGG 5 cut(s) 554, 596, 674, 758, 1213
Esp3I CGTCTC 1 cut(s) 62
FaeI CATG 1 cut(s) 955
FaiI YATR 7 cut(s) 282, 290, 953, 1275, 1317, 1322, 1445
FaqI GGGAC 2 cut(s) 261, 305
FatI CATG 1 cut(s) 951
FauI CCCGC 1 cut(s) 1038
Fnu4HI GCNGC 4 cut(s) 6, 431, 915, 1302
FokI GGATG 2 cut(s) 256, 1355
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 4 cut(s) 6, 431, 915, 1302
FspBI CTAG 4 cut(s) 51, 1064, 1107, 1214
GlaI GCGC 1 cut(s) 374
GluI GCNGC 4 cut(s) 6, 431, 915, 1302
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 1 cut(s) 825
HapII CCGG 2 cut(s) 158, 783
HgaI GACGC 1 cut(s) 263
HhaI GCGC 1 cut(s) 375
Hin1I GRCGYC 1 cut(s) 274
Hin1II CATG 1 cut(s) 955
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HinfI GANTC 2 cut(s) 47, 1238
HpaII CCGG 2 cut(s) 158, 783
HphI GGTGA 8 cut(s) 172, 247, 351, 381, 540, 587, 707, 1169
Hpy188I TCNGA 8 cut(s) 295, 327, 343, 421, 898, 958, 979, 1413
Hpy188III TCNNGA 5 cut(s) 873, 1118, 1193, 1242, 1259
HpyAV CCTTC 6 cut(s) 329, 678, 903, 1078, 1132, 1228
HpyCH4IV ACGT 1 cut(s) 1078
HpyCH4V TGCA 4 cut(s) 620, 701, 1100, 1304
HpyF3I CTNAG 7 cut(s) 168, 393, 864, 1002, 1059, 1257, 1410
HpySE526I ACGT 1 cut(s) 1078
Hsp92I GRCGYC 1 cut(s) 274
Hsp92II CATG 1 cut(s) 955
HspAI GCGC 1 cut(s) 373
KspI CCGCGG 1 cut(s) 368
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 3 cut(s) 344, 450, 964
Lsp1109I GCAGC 2 cut(s) 926, 1288
LweI GCATC 3 cut(s) 274, 688, 1288
MaeI CTAG 4 cut(s) 51, 1064, 1107, 1214
MaeII ACGT 1 cut(s) 1078
MaeIII GTNAC 3 cut(s) 357, 1157, 1358
MflI RGATCY 9 cut(s) 463, 502, 541, 583, 622, 661, 703, 745, 889
MhlI GDGCHC 1 cut(s) 271
MluCI AATT 1 cut(s) 187
MmeI TCCRAC 2 cut(s) 936, 996
MroXI GAANNNNTTC 2 cut(s) 406, 1200
MseI TTAA 5 cut(s) 24, 123, 263, 315, 1283
MslI CAYNNNNRTG 2 cut(s) 233, 1341
MspA1I CMGCKG 2 cut(s) 194, 367
MspI CCGG 2 cut(s) 158, 783
MspR9I CCNGG 3 cut(s) 399, 783, 1329
MvaI CCWGG 2 cut(s) 399, 1329
MvnI CGCG 1 cut(s) 367
NciI CCSGG 1 cut(s) 783
NlaIII CATG 1 cut(s) 955
NlaIV GGNNCC 3 cut(s) 321, 340, 891
NmeAIII GCCGAG 3 cut(s) 250, 459, 851
NmuCI GTSAC 2 cut(s) 357, 1157
NspI RCATGY 1 cut(s) 955
PciSI GCTCTTC 1 cut(s) 92
PdmI GAANNNNTTC 2 cut(s) 406, 1200
PfeI GAWTC 2 cut(s) 47, 1238
PflMI CCANNNNNTGG 1 cut(s) 809
PinAI ACCGGT 1 cut(s) 157
PkrI GCNGC 4 cut(s) 7, 432, 916, 1303
PpuMI RGGWCCY 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 2 cut(s) 397, 1327
PspEI GGTNACC 1 cut(s) 1157
PspGI CCWGG 2 cut(s) 397, 1327
PspN4I GGNNCC 3 cut(s) 321, 340, 891
PspPI GGNCC 2 cut(s) 319, 785
PspPPI RGGWCCY 1 cut(s) 319
PsuI RGATCY 9 cut(s) 463, 502, 541, 583, 622, 661, 703, 745, 889
PvuII CAGCTG 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 233, 1341
SacII CCGCGG 1 cut(s) 368
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 5 cut(s) 24, 123, 263, 315, 1283
SatI GCNGC 4 cut(s) 6, 431, 915, 1302
Sau96I GGNCC 2 cut(s) 319, 785
ScrFI CCNGG 3 cut(s) 399, 783, 1329
SduI GDGCHC 1 cut(s) 271
SfaNI GCATC 3 cut(s) 274, 688, 1288
Sfr303I CCGCGG 1 cut(s) 368
SgrBI CCGCGG 1 cut(s) 368
SinI GGWCC 2 cut(s) 319, 785
SmiMI CAYNNNNRTG 2 cut(s) 233, 1341
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 1 cut(s) 187
SsiI CCGC 9 cut(s) 5, 107, 365, 367, 431, 539, 607, 646, 1045
SspMI CTAG 4 cut(s) 51, 1064, 1107, 1214
StyD4I CCNGG 3 cut(s) 397, 781, 1327
StyI CCWWGG 5 cut(s) 554, 596, 674, 758, 1213
TaiI ACGT 1 cut(s) 1081
TaqI TCGA 3 cut(s) 410, 872, 1312
TasI AATT 1 cut(s) 187
TauI GCSGC 2 cut(s) 8, 433
TfiI GAWTC 2 cut(s) 47, 1238
Tru1I TTAA 5 cut(s) 24, 123, 263, 315, 1283
Tru9I TTAA 5 cut(s) 24, 123, 263, 315, 1283
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 2 cut(s) 357, 1157
TseI GCWGC 2 cut(s) 914, 1301
Tsp45I GTSAC 2 cut(s) 357, 1157
TspDTI ATGAA 7 cut(s) 150, 237, 260, 756, 1008, 1151, 1247
TspGWI ACGGA 4 cut(s) 1117, 1377, 1402, 1430
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 809
VpaK11BI GGWCC 2 cut(s) 319, 785
XagI CCTNNNNNAGG 1 cut(s) 1212
XceI RCATGY 1 cut(s) 955
XcmI CCANNNNNNNNNTGG 1 cut(s) 1335
XmaJI CCTAGG 1 cut(s) 1213
XmnI GAANNNNTTC 2 cut(s) 406, 1200
XspI CTAG 4 cut(s) 51, 1064, 1107, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.