MD16G1006500.v1.1

BUD13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010092
Physical Location & Seq
Forward (+)
499381 .. 502312
2932 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1006500.v1.1.491

Sequence Viewer

Length: 1482 bp
ATGGCGTCTTCGAGTAAATCTTTGAAAGAGTATTTGAAACGGTATGAAGTGAATAATGAAGAGGATAAGAAGAAAAAGAAGAAGAACAAGAAGCAGAAAACCAAGGCGGAGCCAATGGGCGTTTTAGTGGTTGATGAAGACCCTGTTTGGCAGAAACCGGTGAATGTTGAGGAAGATAATGGTGACAATTCATCGGATGAAGACAAACCTGTAGTTGATGAGGACATTGAGATTAAGAGGATGAAGAGACTTCAACAATTGAGGGCCAGACGTCCCTTTAATTCCATATCCGAAGATGGAAGCGGTTGGGTTACAGTTGGAGACTCTCCTCCTAAACAGTCAAACTCTGATTTATCTCCACCTCGTAGGGAGAGGAGTTTGGTTCTGAAATCTGAACCAATGTCTCCGAAAAATAGGAGGGAAGATGCTGACTTGTCACCTCCACGACAGAGACGGAAGCGCCACCACACCCCGTCACCCGAGCCTGAAACAAATCACGAGTACTTGCCTCCTCCTCCTGGATCAGACCTTTCACCCCCAAGGAAAAGGAGGCAGGATGTTGATACAGATCTGTCTTCACCAAGGAAGAGCAGGAAGGATAGTAATAAAGATCTGTCTCCACCAAGGAAGGGCAGGAAGTACGCTGATTCAGATCTTTCTCCACCGAGGAAAGGTAGGAACAATGTTGATGCTGATCTTTCTCCACCAAGGAATAGCAGGAGGGATGTTGATACTGATCTTTCCCCACCAAGGAAAAGCAGGAAGGATGTTGATGGTGATCTTTCCCCGCCAAGGAATAAAAAGCAAGTTACTCCGCACCCTCGTAAAGAGCAACCAAAAGTTGGTCTGATCTCTGGCCGAGAGATTAGTGACGAGATCAATAAAACGAAGAAGGATGAGTGGTTAAGATTTCAACAGATGGATCCTTCTGTAAGTGGGAGAGAGGCAGTACCTATATATCGTGATAAGAAAACAGGGGAACGCATTTCGAAGGAGGAGCTTTTGAAATCGCAAAGGAAAATAGATGAAAAACCAAAGGAGATAAAGTTGGAATGGGGCAAAGGCTTGGCCCAAAAGCGAGAAGCTGAAACTAAGCTGGAGGAATTAGAACTTGAGAAGGATAAGCCTTTTGCGCGGACTAGAGATGATCCAGAACTCGACAAAATATTGAAAGAGAGGACAAGGTGGGGCGATCCCATGGCACATTTGGTAAAGAAAAAGCATTCCGAGCCAGTTCTCTCTAACTTGGGGGATGATGAGAAAATGAAGGAATCTGGATTCATTATTCCCCAGGATATTCCTGTTCACAGCTGGTTAAAAAGAGGATTGGATGCTGCACCAAATCGATACGGTATAAGACCAGGAAGACATTGGGATGGAGTTGATCGCAGTAACGGATTTGAGAAGGGAATGTTCAAAAGGAAGAATGAGAAACAAGCCACGGAGGCAGAAGCTTATCTTTGGTCTGTGGCGGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bud13

Protein Analysis

494

Amino Acids

56.86

Weight (kDa)

9.54

Isoelectric Point (pI)

62.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bud13 PF09736 319 - 476 4.8e-41 Pre-mRNA-splicing factor of RES complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 274
AccB7I CCANNNNNTGG 1 cut(s) 842
AccII CGCG 1 cut(s) 1135
AciI CCGC 6 cut(s) 107, 303, 788, 815, 1135, 1472
AclWI GGATC 5 cut(s) 529, 917, 930, 1142, 1187
AcoI YGGCCR 1 cut(s) 856
AcyI GRCGYC 2 cut(s) 5, 271
AfaI GTAC 3 cut(s) 503, 641, 951
AfiI CCNNNNNNNGG 6 cut(s) 518, 629, 671, 750, 792, 842
AgeI ACCGGT 1 cut(s) 157
AgsI TTSAA 7 cut(s) 25, 37, 254, 914, 1006, 1171, 1417
AjnI CCWGG 3 cut(s) 517, 1290, 1360
AluBI AGCT 5 cut(s) 1000, 1085, 1096, 1311, 1454
AluI AGCT 5 cut(s) 1000, 1085, 1096, 1311, 1454
Alw26I GTCTC 5 cut(s) 241, 315, 408, 445, 621
AlwI GGATC 5 cut(s) 529, 917, 930, 1142, 1187
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 3 cut(s) 264, 856, 1068
ApeKI GCWGC 1 cut(s) 1334
AsiGI ACCGGT 1 cut(s) 157
AspLEI GCGC 2 cut(s) 462, 1135
AspS9I GGNCC 2 cut(s) 264, 1069
AsuHPI GGTGA 7 cut(s) 172, 194, 429, 468, 525, 570, 788
AsuII TTCGAA 1 cut(s) 989
AvaI CYCGRG 1 cut(s) 479
BamHI GGATCC 1 cut(s) 922
BauI CACGAG 1 cut(s) 497
BbsI GAAGAC 4 cut(s) 144, 207, 567, 1372
BbvI GCAGC 1 cut(s) 1321
BccI CCATC 4 cut(s) 290, 767, 913, 1370
BciT130I CCWGG 3 cut(s) 519, 1292, 1362
BcoDI GTCTC 5 cut(s) 241, 315, 408, 445, 621
BfaI CTAG 1 cut(s) 1140
BfmI CTRYAG 1 cut(s) 210
BfoI RGCGCY 1 cut(s) 463
BglI GCCNNNNNGGC 1 cut(s) 1445
BglII AGATCT 3 cut(s) 568, 610, 652
BisI GCNGC 1 cut(s) 1335
BlsI GCNGC 1 cut(s) 1336
BmcAI AGTACT 1 cut(s) 503
Bme1390I CCNGG 3 cut(s) 519, 1292, 1362
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 2 cut(s) 264, 1069
BmiI GGNNCC 2 cut(s) 111, 924
BmrFI CCNGG 3 cut(s) 519, 1292, 1362
BmsI GCATC 3 cut(s) 415, 679, 1321
BpiI GAAGAC 4 cut(s) 144, 207, 567, 1372
BpmI CTGGAG 1 cut(s) 1118
Bpu14I TTCGAA 1 cut(s) 989
BpuEI CTTGAG 1 cut(s) 1133
Bsa29I ATCGAT 1 cut(s) 1345
BsaBI GATNNNNATC 5 cut(s) 567, 651, 693, 735, 777
BsaHI GRCGYC 2 cut(s) 5, 271
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 1031, 1061
Bsc4I CCNNNNNNNGG 6 cut(s) 518, 629, 671, 750, 792, 842
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 1232
Bse8I GATNNNNATC 5 cut(s) 567, 651, 693, 735, 777
BseBI CCWGG 3 cut(s) 519, 1292, 1362
BseCI ATCGAT 1 cut(s) 1345
BseGI GGATG 9 cut(s) 202, 246, 562, 730, 772, 901, 1258, 1336, 1381
BseJI GATNNNNATC 5 cut(s) 567, 651, 693, 735, 777
BseLI CCNNNNNNNGG 6 cut(s) 518, 629, 671, 750, 792, 842
BseNI ACTGG 1 cut(s) 1232
BseRI GAGGAG 5 cut(s) 318, 388, 501, 504, 1010
BseXI GCAGC 1 cut(s) 1321
BsgI GTGCAG 1 cut(s) 1320
Bsh1236I CGCG 1 cut(s) 1135
BshFI GGCC 3 cut(s) 266, 858, 1070
BshTI ACCGGT 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 1345
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 1 cut(s) 258
BslI CCNNNNNNNGG 6 cut(s) 518, 629, 671, 750, 792, 842
BsmAI GTCTC 5 cut(s) 241, 315, 408, 445, 621
BsmBI CGTCTC 1 cut(s) 445
BsmFI GGGAC 1 cut(s) 258
BsmI GAATGC 1 cut(s) 1222
BsnI GGCC 3 cut(s) 266, 858, 1070
BsoBI CYCGRG 1 cut(s) 479
Bsp119I TTCGAA 1 cut(s) 989
Bsp19I CCATGG 1 cut(s) 1197
BspACI CCGC 6 cut(s) 107, 303, 788, 815, 1135, 1472
BspANI GGCC 3 cut(s) 266, 858, 1070
BspDI ATCGAT 1 cut(s) 1345
BspFNI CGCG 1 cut(s) 1135
BspLI GGNNCC 2 cut(s) 111, 924
BspPI GGATC 5 cut(s) 529, 917, 930, 1142, 1187
BspQI GCTCTTC 1 cut(s) 581
BspT104I TTCGAA 1 cut(s) 989
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 1232
BssAI RCCGGY 1 cut(s) 157
BssNI GRCGYC 2 cut(s) 5, 271
BssSI CACGAG 1 cut(s) 497
BssT1I CCWWGG 8 cut(s) 102, 539, 581, 623, 707, 749, 791, 1197
Bst2BI CACGAG 1 cut(s) 497
Bst2UI CCWGG 3 cut(s) 519, 1292, 1362
Bst4CI ACNGT 4 cut(s) 42, 316, 339, 1352
Bst6I CTCTTC 3 cut(s) 54, 239, 581
BstACI GRCGYC 2 cut(s) 5, 271
BstBI TTCGAA 1 cut(s) 989
BstDEI CTNAG 1 cut(s) 1092
BstDSI CCRYGG 2 cut(s) 1197, 1440
BstF5I GGATG 9 cut(s) 202, 246, 562, 730, 772, 901, 1258, 1336, 1381
BstFNI CGCG 1 cut(s) 1135
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 2 cut(s) 462, 1135
BstMAI GTCTC 5 cut(s) 241, 315, 408, 445, 621
BstMWI GCNNNNNNNGC 3 cut(s) 1132, 1228, 1445
BstNI CCWGG 3 cut(s) 519, 1292, 1362
BstSCI CCNGG 3 cut(s) 517, 1290, 1360
BstSFI CTRYAG 1 cut(s) 210
BstUI CGCG 1 cut(s) 1135
BstV1I GCAGC 1 cut(s) 1321
BstV2I GAAGAC 4 cut(s) 144, 207, 567, 1372
BstX2I RGATCY 4 cut(s) 568, 610, 652, 922
BstYI RGATCY 4 cut(s) 568, 610, 652, 922
Bsu15I ATCGAT 1 cut(s) 1345
BsuRI GGCC 3 cut(s) 266, 858, 1070
BsuTUI ATCGAT 1 cut(s) 1345
BtgI CCRYGG 2 cut(s) 1197, 1440
BtsCI GGATG 9 cut(s) 202, 246, 562, 730, 772, 901, 1258, 1336, 1381
CfoI GCGC 2 cut(s) 462, 1135
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 264, 1069
ClaI ATCGAT 1 cut(s) 1345
Csp6I GTAC 3 cut(s) 502, 640, 950
CspAI ACCGGT 1 cut(s) 157
CviAII CATG 1 cut(s) 1198
CviQI GTAC 3 cut(s) 502, 640, 950
DdeI CTNAG 1 cut(s) 1092
EaeI YGGCCR 1 cut(s) 856
Eam1104I CTCTTC 3 cut(s) 54, 239, 581
EarI CTCTTC 3 cut(s) 54, 239, 581
EciI GGCGGA 1 cut(s) 122
Eco130I CCWWGG 8 cut(s) 102, 539, 581, 623, 707, 749, 791, 1197
Eco88I CYCGRG 1 cut(s) 479
EcoRII CCWGG 3 cut(s) 517, 1290, 1360
EcoT14I CCWWGG 8 cut(s) 102, 539, 581, 623, 707, 749, 791, 1197
ErhI CCWWGG 8 cut(s) 102, 539, 581, 623, 707, 749, 791, 1197
Esp3I CGTCTC 1 cut(s) 445
FaeI CATG 1 cut(s) 1201
FaiI YATR 7 cut(s) 45, 287, 956, 958, 1199, 1355, 1478
FalI AAGNNNNNCTT 2 cut(s) 1443, 1475
FaqI GGGAC 1 cut(s) 258
FatI CATG 1 cut(s) 1197
FauI CCCGC 1 cut(s) 795
Fnu4HI GCNGC 1 cut(s) 1335
FokI GGATG 9 cut(s) 209, 253, 569, 737, 779, 908, 1265, 1343, 1388
Fsp4HI GCNGC 1 cut(s) 1335
FspBI CTAG 1 cut(s) 1140
GlaI GCGC 2 cut(s) 461, 1134
GluI GCNGC 1 cut(s) 1335
GsuI CTGGAG 1 cut(s) 1118
HaeII RGCGCY 1 cut(s) 463
HaeIII GGCC 3 cut(s) 266, 858, 1070
HapII CCGG 1 cut(s) 158
HhaI GCGC 2 cut(s) 462, 1135
Hin1I GRCGYC 2 cut(s) 5, 271
Hin1II CATG 1 cut(s) 1201
Hin6I GCGC 2 cut(s) 460, 1133
HinP1I GCGC 2 cut(s) 460, 1133
HindIII AAGCTT 1 cut(s) 1452
HinfI GANTC 4 cut(s) 323, 647, 1271, 1278
HpaII CCGG 1 cut(s) 158
HphI GGTGA 7 cut(s) 172, 194, 429, 468, 525, 570, 788
Hpy166II GTNNAC 1 cut(s) 1306
Hpy188III TCNNGA 4 cut(s) 497, 962, 1151, 1275
Hpy8I GTNNAC 1 cut(s) 1306
HpyAV CCTTC 9 cut(s) 589, 622, 757, 886, 936, 985, 1111, 1261, 1399
HpyCH4III ACNGT 4 cut(s) 42, 316, 339, 1352
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 1 cut(s) 1337
HpyF10VI GCNNNNNNNGC 3 cut(s) 1132, 1228, 1445
HpyF3I CTNAG 1 cut(s) 1092
HpySE526I ACGT 1 cut(s) 271
Hsp92I GRCGYC 2 cut(s) 5, 271
Hsp92II CATG 1 cut(s) 1201
HspAI GCGC 2 cut(s) 460, 1133
LguI GCTCTTC 1 cut(s) 581
LmnI GCTCC 2 cut(s) 109, 997
Lsp1109I GCAGC 1 cut(s) 1321
LweI GCATC 3 cut(s) 415, 679, 1321
MaeI CTAG 1 cut(s) 1140
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 7 cut(s) 182, 310, 435, 474, 808, 869, 1391
MfeI CAATTG 1 cut(s) 257
MflI RGATCY 4 cut(s) 568, 610, 652, 922
MluCI AATT 4 cut(s) 187, 257, 280, 1103
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 2 cut(s) 298, 1029
MseI TTAA 4 cut(s) 234, 279, 905, 1316
MslI CAYNNNNRTG 1 cut(s) 1374
MspA1I CMGCKG 1 cut(s) 1311
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 3 cut(s) 519, 1292, 1362
MunI CAATTG 1 cut(s) 257
Mva1269I GAATGC 1 cut(s) 1222
MvaI CCWGG 3 cut(s) 519, 1292, 1362
MvnI CGCG 1 cut(s) 1135
MwoI GCNNNNNNNGC 3 cut(s) 1132, 1228, 1445
NcoI CCATGG 1 cut(s) 1197
NlaIII CATG 1 cut(s) 1201
NlaIV GGNNCC 2 cut(s) 111, 924
NmeAIII GCCGAG 1 cut(s) 884
NmuCI GTSAC 4 cut(s) 182, 435, 474, 869
NspV TTCGAA 1 cut(s) 989
PciSI GCTCTTC 1 cut(s) 581
PctI GAATGC 1 cut(s) 1222
PfeI GAWTC 3 cut(s) 647, 1271, 1278
PflMI CCANNNNNTGG 1 cut(s) 842
PfoI TCCNGGA 1 cut(s) 517
PinAI ACCGGT 1 cut(s) 157
PkrI GCNGC 1 cut(s) 1336
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
Psp6I CCWGG 3 cut(s) 517, 1290, 1360
PspGI CCWGG 3 cut(s) 517, 1290, 1360
PspN4I GGNNCC 2 cut(s) 111, 924
PspPI GGNCC 2 cut(s) 264, 1069
PsuI RGATCY 4 cut(s) 568, 610, 652, 922
PvuII CAGCTG 1 cut(s) 1311
RsaI GTAC 3 cut(s) 503, 641, 951
RsaNI GTAC 3 cut(s) 502, 640, 950
RseI CAYNNNNRTG 1 cut(s) 1374
SapI GCTCTTC 1 cut(s) 581
SaqAI TTAA 4 cut(s) 234, 279, 905, 1316
SatI GCNGC 1 cut(s) 1335
Sau96I GGNCC 2 cut(s) 264, 1069
ScaI AGTACT 1 cut(s) 503
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 3 cut(s) 519, 1292, 1362
SfaNI GCATC 3 cut(s) 415, 679, 1321
SfcI CTRYAG 1 cut(s) 210
SfuI TTCGAA 1 cut(s) 989
SmiMI CAYNNNNRTG 1 cut(s) 1374
SmlI CTYRAG 1 cut(s) 1112
SmoI CTYRAG 1 cut(s) 1112
Sse9I AATT 4 cut(s) 187, 257, 280, 1103
SsiI CCGC 6 cut(s) 107, 303, 788, 815, 1135, 1472
SspI AATATT 1 cut(s) 1167
SspMI CTAG 1 cut(s) 1140
StyD4I CCNGG 3 cut(s) 517, 1290, 1360
StyI CCWWGG 8 cut(s) 102, 539, 581, 623, 707, 749, 791, 1197
TaaI ACNGT 4 cut(s) 42, 316, 339, 1352
TaiI ACGT 1 cut(s) 274
TaqI TCGA 4 cut(s) 11, 989, 1158, 1345
TasI AATT 4 cut(s) 187, 257, 280, 1103
TatI WGTACW 1 cut(s) 501
TfiI GAWTC 3 cut(s) 647, 1271, 1278
Tru1I TTAA 4 cut(s) 234, 279, 905, 1316
Tru9I TTAA 4 cut(s) 234, 279, 905, 1316
TseFI GTSAC 4 cut(s) 182, 435, 474, 869
TseI GCWGC 1 cut(s) 1334
Tsp45I GTSAC 4 cut(s) 182, 435, 474, 869
TspDTI ATGAA 9 cut(s) 60, 72, 150, 180, 213, 257, 1041, 1270, 1280
TspGWI ACGGA 3 cut(s) 469, 1410, 1457
Van91I CCANNNNNTGG 1 cut(s) 842
XcmI CCANNNNNNNNNTGG 2 cut(s) 1204, 1368
XspI CTAG 1 cut(s) 1140
ZraI GACGTC 1 cut(s) 272
ZrmI AGTACT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.