Rorug04G0370000

BUD13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010092
Physical Location & Seq
Forward (+)
54100417 .. 54100800
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0370000.1

Sequence Viewer

Length: 384 bp
ATGCAGCAAGGAAAGGAACCCAACTTTCTTGCTGATGAGGAAGCAAGAGGTCCTAGTAGTCATGAAGAGCCTCAGCCATCTCTTAGGAGATGTAATGACTCGGCCAATGCTGAAGCAGTCGTAACTGATAACAACTCTAAGGAGGCGGATGATGGGTATGCTCCATCAATTGACGATCACAGTTTCATTGACAGCCATGAGCAAGACATTCCAGAAGATTCTGAGCAATCAGAGCTGAAAAGTGGCCGACGAAAACCAGCTAGGGGATGCAAATCTAGATTGTCTAGAACACACTCAGTGAAGGCAGTTGTTGAAGACGCAAAGAAATTTTTAGGAGAAACTCCAGAAGCATCAAATGCAAGTTTGCTCAATGAGAGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bud13

Protein Analysis

127

Amino Acids

13.94

Weight (kDa)

4.9

Isoelectric Point (pI)

61.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 146
AcoI YGGCCR 2 cut(s) 102, 244
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 1 cut(s) 132
AdeI CACNNNGTG 1 cut(s) 298
AfiI CCNNNNNNNGG 1 cut(s) 263
AgsI TTSAA 1 cut(s) 314
AluBI AGCT 2 cut(s) 235, 260
AluI AGCT 2 cut(s) 235, 260
AoxI GGCC 2 cut(s) 102, 244
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 1 cut(s) 326
AspS9I GGNCC 1 cut(s) 50
AvaII GGWCC 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 321
BbvCI CCTCAGC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 16
BccI CCATC 3 cut(s) 85, 146, 172
BfaI CTAG 4 cut(s) 54, 261, 276, 285
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme18I GGWCC 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 50
BmiI GGNNCC 1 cut(s) 18
BmsI GCATC 2 cut(s) 257, 359
BpiI GAAGAC 1 cut(s) 321
BpmI CTGGAG 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 271
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse8I GATNNNNATC 1 cut(s) 271
BseGI GGATG 2 cut(s) 154, 272
BseJI GATNNNNATC 1 cut(s) 271
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMII CTCAG 3 cut(s) 86, 213, 309
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 2 cut(s) 104, 246
BslI CCNNNNNNNGG 1 cut(s) 263
BsnI GGCC 2 cut(s) 104, 246
Bsp143I GATC 1 cut(s) 175
BspACI CCGC 1 cut(s) 146
BspANI GGCC 2 cut(s) 104, 246
BspCNI CTCAG 3 cut(s) 85, 214, 308
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 18
BspQI GCTCTTC 1 cut(s) 60
BssMI GATC 1 cut(s) 175
Bst4CI ACNGT 1 cut(s) 182
Bst6I CTCTTC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 356
BstDEI CTNAG 5 cut(s) 72, 83, 138, 222, 295
BstF5I GGATG 2 cut(s) 154, 272
BstKTI GATC 1 cut(s) 178
BstMBI GATC 1 cut(s) 175
BstMWI GCNNNNNNNGC 2 cut(s) 232, 356
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 321
BsuRI GGCC 2 cut(s) 104, 246
BtsCI GGATG 2 cut(s) 154, 272
BtsIMutI CAGTG 1 cut(s) 303
CciI TCATGA 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 50
CseI GACGC 1 cut(s) 326
CviAII CATG 2 cut(s) 62, 197
CviJI RGCY 7 cut(s) 70, 76, 104, 195, 235, 246, 260
CviKI_1 RGCY 7 cut(s) 70, 76, 104, 195, 235, 246, 260
DdeI CTNAG 5 cut(s) 72, 83, 138, 222, 295
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
DraIII CACNNNGTG 1 cut(s) 298
EaeI YGGCCR 2 cut(s) 102, 244
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
EciI GGCGGA 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 132
EcoO109I RGGNCCY 1 cut(s) 50
FaeI CATG 2 cut(s) 65, 200
FaiI YATR 3 cut(s) 63, 159, 198
FatI CATG 2 cut(s) 61, 196
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 2 cut(s) 161, 279
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 4 cut(s) 54, 261, 276, 285
GluI GCNGC 1 cut(s) 5
GsuI CTGGAG 1 cut(s) 327
HaeIII GGCC 2 cut(s) 104, 246
HgaI GACGC 1 cut(s) 326
Hin1II CATG 2 cut(s) 65, 200
HinfI GANTC 2 cut(s) 98, 218
Hpy188I TCNGA 2 cut(s) 223, 232
Hpy188III TCNNGA 5 cut(s) 62, 212, 276, 285, 344
Hpy99I CGWCG 1 cut(s) 252
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4V TGCA 3 cut(s) 4, 270, 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 232, 356
HpyF3I CTNAG 5 cut(s) 72, 83, 138, 222, 295
Hsp92II CATG 2 cut(s) 65, 200
Kzo9I GATC 1 cut(s) 175
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 1 cut(s) 166
LpnPI CCDG 3 cut(s) 225, 270, 357
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 2 cut(s) 257, 359
MaeI CTAG 4 cut(s) 54, 261, 276, 285
MaeIII GTNAC 1 cut(s) 121
MalI GATC 1 cut(s) 177
MboI GATC 1 cut(s) 175
MboII GAAGA 3 cut(s) 77, 227, 326
MfeI CAATTG 1 cut(s) 168
MluCI AATT 2 cut(s) 168, 326
MlyI GAGTC 1 cut(s) 92
MnlI CCTC 4 cut(s) 31, 41, 81, 136
MunI CAATTG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 232, 356
NdeII GATC 1 cut(s) 175
NlaIII CATG 2 cut(s) 65, 200
NlaIV GGNNCC 1 cut(s) 18
NmeAIII GCCGAG 1 cut(s) 80
PagI TCATGA 1 cut(s) 61
PciSI GCTCTTC 1 cut(s) 60
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PpuMI RGGWCCY 1 cut(s) 50
Psp5II RGGWCCY 1 cut(s) 50
PspN4I GGNNCC 1 cut(s) 18
PspPI GGNCC 1 cut(s) 50
PspPPI RGGWCCY 1 cut(s) 50
SapI GCTCTTC 1 cut(s) 60
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 1 cut(s) 175
Sau96I GGNCC 1 cut(s) 50
SchI GAGTC 1 cut(s) 92
SetI ASST 3 cut(s) 52, 237, 262
SfaNI GCATC 2 cut(s) 257, 359
SinI GGWCC 1 cut(s) 50
Sse9I AATT 2 cut(s) 168, 326
SsiI CCGC 1 cut(s) 146
SspMI CTAG 4 cut(s) 54, 261, 276, 285
TaaI ACNGT 1 cut(s) 182
TasI AATT 2 cut(s) 168, 326
TfiI GAWTC 1 cut(s) 218
TscAI CASTG 1 cut(s) 303
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 2 cut(s) 78, 175
TspRI CASTG 1 cut(s) 303
VpaK11BI GGWCC 1 cut(s) 50
XapI RAATTY 1 cut(s) 326
XbaI TCTAGA 2 cut(s) 275, 284
XspI CTAG 4 cut(s) 54, 261, 276, 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.