Rh4DG439800

BUD13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010092
Physical Location & Seq
Reverse (-)
64185307 .. 64188593
3287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG439800.1

Sequence Viewer

Length: 1488 bp
ATGGCGGCTTCCACGAAATCGCTTAAAGAGTATTTGAAACGATACGAATCTAGTAATAACGAGGACGATACGAAGAAGAAGAGGAAGAAGAAGCAGAAGAGCAAGGCGGAGGCAATGGGGGTTAAAGTGGTGGATGAGGACCCTGTGTGGCAGAAACCGGTGAAAATTGAGGGAGAAGAAGATAACAATTCAGCTGACGAAGAGGAGAAGCCTTTAGTTGATGAAGACATTGAGGTGAAGAGGATGAAGAGGCTTGAGTTATTAAGGGCGCGGCGGCCCTATGATGCTATATCTGAAGATGGCAGTGGTTGGGTTAAGGTCCCTCTGAAAAGAGAAGGCTCCGAAGAGGCTGACTTGTCACCTCCGCGGAAGCGCTATCATTCTCCCTCACCTGAGCCTGGAATGGCTTCGAGGGGATCAGACCTTTCGCCGCCGAGGAAAAGTAGGAGGGGTGGTGATGAAGATCTTTCTCCACCGAGGAAGAGTAGGAGCAACGTTGAAGATCTTTCTCCACCGAGGAAGAGTAGGAGCAATGTTGAAGATCTTTCTCCACCGAGGAAGAGGAGGAACAATGTTGGGGATCTTTCTCCACCAAGGAAGAGTAGGAACAATGGTGATGGAGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGTGGCAGATCTTTCTCCACCGAGGAACAGGCGGAACAATGTTGAAGATCTTTCTCCACCAAGGAAGAGAAGGAACAATGGTGATGCAGATCTTTCTCCACCAAGGAAAAGTAGGAGGGATATTGATGAAGATCTATCTCCACCAAGGAAAAAGAAGCAAGTATCTCCTGGTCCTTTGAAAGAGAAGCCAAAAAGTGGTTTGATATCTGGCCGAGATATCAGCGAAGAGATTTCTAAAACGAAGAGAGCTGAGCAGTCGAGATTTGAACAGATGGATCCTTCTGAAACTGGGCAACAAGCAGCAGTTGTATTTCGTAATAAGAAAACAGGAGAACGCATGTCGGAGGAGGAGCGATTGAAATCAGAAAAGAAACTTGATGAAAAGCCTAAGGAGATAAAGTTGGAATGGGGTAAGGGCTTGGCTCAAAAGCGGGAAGCTGAAGCTAAGCTAGAGGAATTAGAACGTGAGAAGGAAAAACCATTTGCACGGACTAGAGATGATCCAGAACTTGACAAAATGATGAAGGAGAGAGTAAGATGGGGTGACCCAATGGCACATTTGGTAAAGAAAAAGTATCCTGAAGCAGTTCTCCCTAACCTAGGGGATAGCGAGAAAATGAAGGACTCTGGATTTATTATCCCTCAGGATATTCCTAATCATAGCTGGTTAAAAAGAGGATTAGATGCTGCACCAAATCGATATGGTATAAAACCAGGGAGACATTGGGATGGAGTTGATCGCAGTAACGGATTTGAGAAAGGATTGTTCAAACGGACTAACGAGAAACAAGCTACTCAGACGGAGGCTTATCTTTGGTCTGTCGCAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bud13

Protein Analysis

495

Amino Acids

56.64

Weight (kDa)

9.61

Isoelectric Point (pI)

68.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bud13 PF09736 320 - 478 5.8e-40 Pre-mRNA-splicing factor of RES complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 848
AccII CGCG 2 cut(s) 271, 367
AciI CCGC 9 cut(s) 5, 107, 271, 274, 365, 367, 431, 685, 1084
AclI AACGTT 1 cut(s) 495
AclWI GGATC 5 cut(s) 424, 588, 923, 936, 1148
AcoI YGGCCR 1 cut(s) 862
AcuI CTGAAG 3 cut(s) 315, 1113, 1254
AfeI AGCGCT 1 cut(s) 374
AfiI CCNNNNNNNGG 3 cut(s) 398, 848, 1253
AgeI ACCGGT 1 cut(s) 157
AgsI TTSAA 8 cut(s) 37, 500, 539, 698, 832, 920, 1012, 1423
AjnI CCWGG 3 cut(s) 397, 820, 1366
AluBI AGCT 7 cut(s) 194, 902, 1091, 1097, 1102, 1317, 1445
AluI AGCT 7 cut(s) 194, 902, 1091, 1097, 1102, 1317, 1445
Alw26I GTCTC 1 cut(s) 1366
AlwI GGATC 5 cut(s) 424, 588, 923, 936, 1148
Aor51HI AGCGCT 1 cut(s) 374
AoxI GGCC 2 cut(s) 275, 862
ApeKI GCWGC 2 cut(s) 953, 1340
AsiGI ACCGGT 1 cut(s) 157
Asp700I GAANNNNTTC 2 cut(s) 406, 1239
AspA2I CCTAGG 1 cut(s) 1252
AspLEI GCGC 2 cut(s) 271, 375
AspS9I GGNCC 4 cut(s) 139, 276, 319, 824
AsuHPI GGTGA 8 cut(s) 172, 247, 351, 381, 467, 626, 746, 1208
AvaII GGWCC 3 cut(s) 139, 319, 824
AvrII CCTAGG 1 cut(s) 1252
AxyI CCTNAGG 2 cut(s) 1041, 1296
BamHI GGATCC 1 cut(s) 928
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 965, 1327
BccI CCATC 5 cut(s) 293, 611, 919, 1185, 1376
BciT130I CCWGG 3 cut(s) 399, 822, 1368
BciVI GTATCC 1 cut(s) 1239
BcoDI GTCTC 1 cut(s) 1366
BfaI CTAG 4 cut(s) 51, 1103, 1146, 1253
BfoI RGCGCY 1 cut(s) 376
BfuI GTATCC 1 cut(s) 1239
BglII AGATCT 8 cut(s) 463, 502, 541, 622, 661, 700, 742, 784
BisI GCNGC 6 cut(s) 6, 272, 275, 431, 954, 1341
BlnI CCTAGG 1 cut(s) 1252
BlpI GCTNAGC 2 cut(s) 903, 1098
BlsI GCNGC 6 cut(s) 7, 273, 276, 432, 955, 1342
Bme1390I CCNGG 3 cut(s) 399, 822, 1368
Bme18I GGWCC 3 cut(s) 139, 319, 824
BmgT120I GGNCC 4 cut(s) 139, 276, 319, 824
BmiI GGNNCC 4 cut(s) 141, 321, 340, 930
BmrFI CCNGG 3 cut(s) 399, 822, 1368
BmrI ACTGGG 1 cut(s) 951
BmsI GCATC 3 cut(s) 274, 727, 1327
BmuI ACTGGG 1 cut(s) 951
BpiI GAAGAC 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 393
Bpu1102I GCTNAGC 2 cut(s) 903, 1098
BpuEI CTTGAG 1 cut(s) 275
Bsa29I ATCGAT 1 cut(s) 1351
BsaBI GATNNNNATC 6 cut(s) 46, 462, 621, 741, 783, 1012
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 4 cut(s) 439, 469, 1037, 1067
Bsc4I CCNNNNNNNGG 3 cut(s) 398, 848, 1253
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 946
Bse21I CCTNAGG 2 cut(s) 1041, 1296
Bse3DI GCAATG 2 cut(s) 120, 538
Bse8I GATNNNNATC 6 cut(s) 46, 462, 621, 741, 783, 1012
BseBI CCWGG 3 cut(s) 399, 822, 1368
BseCI ATCGAT 1 cut(s) 1351
BseGI GGATG 3 cut(s) 139, 249, 1387
BseJI GATNNNNATC 6 cut(s) 46, 462, 621, 741, 783, 1012
BseLI CCNNNNNNNGG 3 cut(s) 398, 848, 1253
BseMI GCAATG 2 cut(s) 120, 538
BseMII CTCAG 4 cut(s) 384, 894, 1310, 1463
BseNI ACTGG 1 cut(s) 946
BseRI GAGGAG 4 cut(s) 218, 577, 1013, 1016
BseXI GCAGC 2 cut(s) 965, 1327
BsgI GTGCAG 1 cut(s) 1326
Bsh1236I CGCG 2 cut(s) 271, 367
BshFI GGCC 2 cut(s) 277, 864
BshTI ACCGGT 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 1351
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 3 cut(s) 398, 848, 1253
BsmAI GTCTC 1 cut(s) 1366
BsmFI GGGAC 1 cut(s) 305
BsnI GGCC 2 cut(s) 277, 864
Bsp1720I GCTNAGC 2 cut(s) 903, 1098
BspACI CCGC 9 cut(s) 5, 107, 271, 274, 365, 367, 431, 685, 1084
BspANI GGCC 2 cut(s) 277, 864
BspCNI CTCAG 4 cut(s) 385, 895, 1309, 1462
BspDI ATCGAT 1 cut(s) 1351
BspFNI CGCG 2 cut(s) 271, 367
BspLI GGNNCC 4 cut(s) 141, 321, 340, 930
BspPI GGATC 5 cut(s) 424, 588, 923, 936, 1148
BspQI GCTCTTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 120, 538
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 946
BssAI RCCGGY 1 cut(s) 157
BssT1I CCWWGG 5 cut(s) 593, 713, 755, 797, 1252
Bst2UI CCWGG 3 cut(s) 399, 822, 1368
BstDEI CTNAG 6 cut(s) 393, 903, 1041, 1098, 1296, 1449
BstDSI CCRYGG 1 cut(s) 365
BstEII GGTNACC 1 cut(s) 1196
BstENI CCTNNNNNAGG 1 cut(s) 1251
BstF5I GGATG 3 cut(s) 139, 249, 1387
BstFNI CGCG 2 cut(s) 271, 367
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 2 cut(s) 271, 375
BstMAI GTCTC 1 cut(s) 1366
BstNI CCWGG 3 cut(s) 399, 822, 1368
BstNSI RCATGY 1 cut(s) 994
BstPI GGTNACC 1 cut(s) 1196
BstSCI CCNGG 3 cut(s) 397, 820, 1366
BstUI CGCG 2 cut(s) 271, 367
BstV1I GCAGC 2 cut(s) 965, 1327
BstV2I GAAGAC 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 1351
Bsu36I CCTNAGG 2 cut(s) 1041, 1296
BsuI GTATCC 1 cut(s) 1239
BsuRI GGCC 2 cut(s) 277, 864
BsuTUI ATCGAT 1 cut(s) 1351
BtgI CCRYGG 1 cut(s) 365
BtsCI GGATG 3 cut(s) 139, 249, 1387
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
CfoI GCGC 2 cut(s) 271, 375
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 4 cut(s) 139, 276, 319, 824
Cfr42I CCGCGG 1 cut(s) 368
ClaI ATCGAT 1 cut(s) 1351
CspAI ACCGGT 1 cut(s) 157
CviAII CATG 1 cut(s) 991
DdeI CTNAG 6 cut(s) 393, 903, 1041, 1098, 1296, 1449
EaeI YGGCCR 1 cut(s) 862
EciI GGCGGA 2 cut(s) 122, 700
Eco130I CCWWGG 5 cut(s) 593, 713, 755, 797, 1252
Eco32I GATATC 2 cut(s) 858, 871
Eco47I GGWCC 3 cut(s) 139, 319, 824
Eco47III AGCGCT 1 cut(s) 374
Eco57I CTGAAG 3 cut(s) 315, 1113, 1254
Eco81I CCTNAGG 2 cut(s) 1041, 1296
Eco91I GGTNACC 1 cut(s) 1196
EcoNI CCTNNNNNAGG 1 cut(s) 1251
EcoO109I RGGNCCY 2 cut(s) 139, 319
EcoO65I GGTNACC 1 cut(s) 1196
EcoRII CCWGG 3 cut(s) 397, 820, 1366
EcoRV GATATC 2 cut(s) 858, 871
EcoT14I CCWWGG 5 cut(s) 593, 713, 755, 797, 1252
ErhI CCWWGG 5 cut(s) 593, 713, 755, 797, 1252
FaeI CATG 1 cut(s) 994
FaiI YATR 7 cut(s) 282, 290, 992, 1314, 1356, 1361, 1484
FaqI GGGAC 1 cut(s) 305
FatI CATG 1 cut(s) 990
FauI CCCGC 1 cut(s) 1077
Fnu4HI GCNGC 6 cut(s) 6, 272, 275, 431, 954, 1341
FokI GGATG 3 cut(s) 146, 256, 1394
Fsp4HI GCNGC 6 cut(s) 6, 272, 275, 431, 954, 1341
FspBI CTAG 4 cut(s) 51, 1103, 1146, 1253
GlaI GCGC 2 cut(s) 270, 374
GluI GCNGC 6 cut(s) 6, 272, 275, 431, 954, 1341
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 2 cut(s) 277, 864
HapII CCGG 1 cut(s) 158
HhaI GCGC 2 cut(s) 271, 375
Hin1II CATG 1 cut(s) 994
Hin6I GCGC 2 cut(s) 269, 373
HinP1I GCGC 2 cut(s) 269, 373
HinfI GANTC 2 cut(s) 47, 1277
HpaII CCGG 1 cut(s) 158
HphI GGTGA 8 cut(s) 172, 247, 351, 381, 467, 626, 746, 1208
Hpy188I TCNGA 8 cut(s) 295, 327, 343, 421, 937, 997, 1018, 1452
Hpy188III TCNNGA 5 cut(s) 912, 1157, 1232, 1281, 1298
HpyAV CCTTC 6 cut(s) 329, 717, 942, 1117, 1171, 1267
HpyCH4IV ACGT 2 cut(s) 495, 1117
HpyCH4V TGCA 3 cut(s) 740, 1139, 1343
HpyF3I CTNAG 6 cut(s) 393, 903, 1041, 1098, 1296, 1449
HpySE526I ACGT 2 cut(s) 495, 1117
Hsp92II CATG 1 cut(s) 994
HspAI GCGC 2 cut(s) 269, 373
KspI CCGCGG 1 cut(s) 368
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 4 cut(s) 344, 489, 528, 1003
Lsp1109I GCAGC 2 cut(s) 965, 1327
LweI GCATC 3 cut(s) 274, 727, 1327
MaeI CTAG 4 cut(s) 51, 1103, 1146, 1253
MaeII ACGT 2 cut(s) 495, 1117
MaeIII GTNAC 3 cut(s) 357, 1196, 1397
MluCI AATT 3 cut(s) 165, 187, 1109
MlyI GAGTC 1 cut(s) 1271
MmeI TCCRAC 2 cut(s) 975, 1035
MroXI GAANNNNTTC 2 cut(s) 406, 1239
MseI TTAA 5 cut(s) 24, 123, 263, 315, 1322
MslI CAYNNNNRTG 2 cut(s) 233, 1380
MspA1I CMGCKG 2 cut(s) 194, 367
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 3 cut(s) 399, 822, 1368
MvaI CCWGG 3 cut(s) 399, 822, 1368
MvnI CGCG 2 cut(s) 271, 367
NlaIII CATG 1 cut(s) 994
NlaIV GGNNCC 4 cut(s) 141, 321, 340, 930
NmeAIII GCCGAG 2 cut(s) 459, 890
NmuCI GTSAC 2 cut(s) 357, 1196
NspI RCATGY 1 cut(s) 994
PciSI GCTCTTC 1 cut(s) 92
PdmI GAANNNNTTC 2 cut(s) 406, 1239
PfeI GAWTC 1 cut(s) 47
PflMI CCANNNNNTGG 1 cut(s) 848
PinAI ACCGGT 1 cut(s) 157
PkrI GCNGC 6 cut(s) 7, 273, 276, 432, 955, 1342
PleI GAGTC 1 cut(s) 1271
PpsI GAGTC 1 cut(s) 1271
PpuMI RGGWCCY 2 cut(s) 139, 319
Psp1406I AACGTT 1 cut(s) 495
Psp5II RGGWCCY 2 cut(s) 139, 319
Psp6I CCWGG 3 cut(s) 397, 820, 1366
PspEI GGTNACC 1 cut(s) 1196
PspGI CCWGG 3 cut(s) 397, 820, 1366
PspN4I GGNNCC 4 cut(s) 141, 321, 340, 930
PspPI GGNCC 4 cut(s) 139, 276, 319, 824
PspPPI RGGWCCY 2 cut(s) 139, 319
PvuII CAGCTG 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 233, 1380
SacII CCGCGG 1 cut(s) 368
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 5 cut(s) 24, 123, 263, 315, 1322
SatI GCNGC 6 cut(s) 6, 272, 275, 431, 954, 1341
Sau96I GGNCC 4 cut(s) 139, 276, 319, 824
SchI GAGTC 1 cut(s) 1271
ScrFI CCNGG 3 cut(s) 399, 822, 1368
SfaNI GCATC 3 cut(s) 274, 727, 1327
Sfr303I CCGCGG 1 cut(s) 368
SgrBI CCGCGG 1 cut(s) 368
SinI GGWCC 3 cut(s) 139, 319, 824
SmiMI CAYNNNNRTG 2 cut(s) 233, 1380
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 3 cut(s) 165, 187, 1109
SsiI CCGC 9 cut(s) 5, 107, 271, 274, 365, 367, 431, 685, 1084
SspMI CTAG 4 cut(s) 51, 1103, 1146, 1253
StyD4I CCNGG 3 cut(s) 397, 820, 1366
StyI CCWWGG 5 cut(s) 593, 713, 755, 797, 1252
TaiI ACGT 2 cut(s) 498, 1120
TaqI TCGA 3 cut(s) 410, 911, 1351
TasI AATT 3 cut(s) 165, 187, 1109
TauI GCSGC 4 cut(s) 8, 274, 277, 433
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 5 cut(s) 24, 123, 263, 315, 1322
Tru9I TTAA 5 cut(s) 24, 123, 263, 315, 1322
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 2 cut(s) 357, 1196
TseI GCWGC 2 cut(s) 953, 1340
Tsp45I GTSAC 2 cut(s) 357, 1196
TspDTI ATGAA 7 cut(s) 237, 260, 474, 795, 1047, 1190, 1286
TspGWI ACGGA 4 cut(s) 1156, 1416, 1441, 1469
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 848
VpaK11BI GGWCC 3 cut(s) 139, 319, 824
XagI CCTNNNNNAGG 1 cut(s) 1251
XceI RCATGY 1 cut(s) 994
XcmI CCANNNNNNNNNTGG 1 cut(s) 1374
XmaJI CCTAGG 1 cut(s) 1252
XmnI GAANNNNTTC 2 cut(s) 406, 1239
XspI CTAG 4 cut(s) 51, 1103, 1146, 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.