Rroxscaffold_5G00386760

BUD13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010092
Physical Location & Seq
Reverse (-)
65546721 .. 65550331
3611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386760.1

Sequence Viewer

Length: 1452 bp
ATGGCGGCTTCCACGAAATCGCTTAAAGAGTATTTGAAACGATACGAATCTAGTAATAACGAGGACGATACGAAGAAGAAGAGGAAGAAGAAGCAGAAGAGCAAGGCGGAGGCAATGGGGGTTAAAGTGGTGGATGAGGACCCTGTGTGGCAGAAACCGGTGAAAATTGAGGGAGAAGAAGATAACAATTCAGCTGACGAAGAGGAGAAGCCTTTAGTTGATGAAGACATTGAGGTGAAGAGGATGAAGAGGCTTGAGTTATTAAGGGCGCGGCGGCCCTATGATGCTATATCTGAAGATGGCAGTGGTTGGGTTAAGGTCCCTCTGAAACGAGAAGGCTCCGAAGAGGCGGACTTGTCACCTCCGCGGAAGCGGTATCATTCTCCCTCACCTGAGCCTGGAATGGCTTCGAGGGGATCAGACCTTTCGCCGCCGAGGAAAAGTAGGAGGGGCGGTGATGAAGATCTTTCTCCACCGAGGAAGAGTAGGAGCAATGTTGAAGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGTTGCGGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGGTGATGGAGATCTTTCTCCACCAAGGAAGAGTCGGAACAATGTTGCAGATCTTTCTCCACCGAGGAAGAGTAGGAACAATGGTGATGGAGATCTTTCAATACCAAGGAAGAGAAGGAACAATGGTGATGCAGATCTTTCTCCACCAAGGAAAAGTAGGAGGGGTATTGATGAAGATCTATCTCCACCAAGGAAAAAGAAGCAAGTATCTCCGGGTCCTTTGAAAGAGAAGCCAAAAAGTGGTTTGATATCTGGCCGAGATATCAGCGAAGAGATTTCTAAAACAAAGAGAGCTGAGCAGTCGAGATTTGAACAGATGGATCCTTCTGAAACTGGGCAACAAGCAGCAGTTGTATTTCGTAATAAGAAAACAGGAGAACGCATGTCGGAGGAGGAGCGATTGAAATCAGAAAAGAAACTTGATGAAAAGCCTAAGGAGATAAAGTTGGAATGGGGTAAGGGCTTGGCTCAAAAGCGGGAAGCTGAAGCTAAGCTAGAGGAATTAGAACGTGAGAAGGAAAAACCATTTGCACGGACTAGAGATGATCCAGAACTTGACAAAATGATGAAGGAGAGAGTAAGATGGGGTGACCCAATGGCACATTTGGTAAAGAAAAAGTATCCTGAAGCAGTTCTCCCTAACCTAGGGGATAGCGAGAAAATGAAGGACTCTGGATTTATTATCCCTCAGGATATTCCTAATCATAGCTGGTTAAAAAGAGGATTAGATGCTGCACCAAATCGATATGGTATAAAACCAGGGAGACATTGGGATGGAGTTGATCGCAGTAACGGATTTGAGAAAGGATTGTTCAAACGGACTAACGAGAAACAAGCTACTCAGACGGAGGCTTATCTTTGGTCTGTGGCAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bud13

Protein Analysis

483

Amino Acids

55.02

Weight (kDa)

9.58

Isoelectric Point (pI)

62.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bud13 PF09736 308 - 466 5.6e-40 Pre-mRNA-splicing factor of RES complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 812
AccII CGCG 2 cut(s) 271, 367
AclWI GGATC 5 cut(s) 424, 549, 887, 900, 1112
AcoI YGGCCR 1 cut(s) 826
AcuI CTGAAG 3 cut(s) 315, 1077, 1218
AfiI CCNNNNNNNGG 3 cut(s) 398, 812, 1217
AgeI ACCGGT 1 cut(s) 157
AgsI TTSAA 7 cut(s) 37, 500, 672, 796, 884, 976, 1387
AjnI CCWGG 2 cut(s) 397, 1330
AluBI AGCT 7 cut(s) 194, 866, 1055, 1061, 1066, 1281, 1409
AluI AGCT 7 cut(s) 194, 866, 1055, 1061, 1066, 1281, 1409
Alw26I GTCTC 1 cut(s) 1330
AlwI GGATC 5 cut(s) 424, 549, 887, 900, 1112
AoxI GGCC 2 cut(s) 275, 826
ApeKI GCWGC 2 cut(s) 917, 1304
AsiGI ACCGGT 1 cut(s) 157
Asp700I GAANNNNTTC 2 cut(s) 406, 1203
AspA2I CCTAGG 1 cut(s) 1216
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 4 cut(s) 139, 276, 319, 788
AsuC2I CCSGG 1 cut(s) 786
AsuHPI GGTGA 9 cut(s) 172, 247, 351, 381, 467, 587, 668, 710, 1172
AvaII GGWCC 3 cut(s) 139, 319, 788
AvrII CCTAGG 1 cut(s) 1216
AxyI CCTNAGG 2 cut(s) 1005, 1260
BamHI GGATCC 1 cut(s) 892
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 929, 1291
BccI CCATC 6 cut(s) 293, 572, 653, 883, 1149, 1340
BciT130I CCWGG 2 cut(s) 399, 1332
BciVI GTATCC 1 cut(s) 1203
BcnI CCSGG 1 cut(s) 786
BcoDI GTCTC 1 cut(s) 1330
BfaI CTAG 4 cut(s) 51, 1067, 1110, 1217
BfuI GTATCC 1 cut(s) 1203
BglII AGATCT 7 cut(s) 463, 502, 583, 622, 664, 706, 748
BisI GCNGC 6 cut(s) 6, 272, 275, 431, 918, 1305
BlnI CCTAGG 1 cut(s) 1216
BlpI GCTNAGC 2 cut(s) 867, 1062
BlsI GCNGC 6 cut(s) 7, 273, 276, 432, 919, 1306
Bme1390I CCNGG 3 cut(s) 399, 786, 1332
Bme18I GGWCC 3 cut(s) 139, 319, 788
BmgT120I GGNCC 4 cut(s) 139, 276, 319, 788
BmiI GGNNCC 5 cut(s) 141, 321, 340, 789, 894
BmrFI CCNGG 3 cut(s) 399, 786, 1332
BmrI ACTGGG 1 cut(s) 915
BmsI GCATC 3 cut(s) 274, 691, 1291
BmuI ACTGGG 1 cut(s) 915
BpiI GAAGAC 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 393
Bpu1102I GCTNAGC 2 cut(s) 867, 1062
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 786
Bsa29I ATCGAT 1 cut(s) 1315
BsaBI GATNNNNATC 7 cut(s) 46, 462, 582, 663, 705, 747, 976
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 4 cut(s) 439, 469, 1001, 1031
Bsc4I CCNNNNNNNGG 3 cut(s) 398, 812, 1217
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 910
Bse21I CCTNAGG 2 cut(s) 1005, 1260
Bse3DI GCAATG 2 cut(s) 120, 499
Bse8I GATNNNNATC 7 cut(s) 46, 462, 582, 663, 705, 747, 976
BseBI CCWGG 2 cut(s) 399, 1332
BseCI ATCGAT 1 cut(s) 1315
BseGI GGATG 3 cut(s) 139, 249, 1351
BseJI GATNNNNATC 7 cut(s) 46, 462, 582, 663, 705, 747, 976
BseLI CCNNNNNNNGG 3 cut(s) 398, 812, 1217
BseMI GCAATG 2 cut(s) 120, 499
BseMII CTCAG 4 cut(s) 384, 858, 1274, 1427
BseNI ACTGG 1 cut(s) 910
BseRI GAGGAG 3 cut(s) 218, 977, 980
BseXI GCAGC 2 cut(s) 929, 1291
BsgI GTGCAG 1 cut(s) 1290
Bsh1236I CGCG 2 cut(s) 271, 367
BshFI GGCC 2 cut(s) 277, 828
BshTI ACCGGT 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 1315
BsiSI CCGG 2 cut(s) 158, 785
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 3 cut(s) 398, 812, 1217
BsmAI GTCTC 1 cut(s) 1330
BsmFI GGGAC 1 cut(s) 305
BsnI GGCC 2 cut(s) 277, 828
Bsp1720I GCTNAGC 2 cut(s) 867, 1062
BspANI GGCC 2 cut(s) 277, 828
BspCNI CTCAG 4 cut(s) 385, 859, 1273, 1426
BspDI ATCGAT 1 cut(s) 1315
BspFNI CGCG 2 cut(s) 271, 367
BspLI GGNNCC 5 cut(s) 141, 321, 340, 789, 894
BspPI GGATC 5 cut(s) 424, 549, 887, 900, 1112
BspQI GCTCTTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 120, 499
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 910
BssAI RCCGGY 1 cut(s) 157
BssT1I CCWWGG 5 cut(s) 596, 677, 719, 761, 1216
Bst2UI CCWGG 2 cut(s) 399, 1332
BstDEI CTNAG 6 cut(s) 393, 867, 1005, 1062, 1260, 1413
BstDSI CCRYGG 1 cut(s) 365
BstEII GGTNACC 1 cut(s) 1160
BstENI CCTNNNNNAGG 1 cut(s) 1215
BstF5I GGATG 3 cut(s) 139, 249, 1351
BstFNI CGCG 2 cut(s) 271, 367
BstHHI GCGC 1 cut(s) 271
BstMAI GTCTC 1 cut(s) 1330
BstNI CCWGG 2 cut(s) 399, 1332
BstNSI RCATGY 1 cut(s) 958
BstPI GGTNACC 1 cut(s) 1160
BstSCI CCNGG 3 cut(s) 397, 784, 1330
BstUI CGCG 2 cut(s) 271, 367
BstV1I GCAGC 2 cut(s) 929, 1291
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 9 cut(s) 463, 502, 541, 583, 622, 664, 706, 748, 892
BstYI RGATCY 9 cut(s) 463, 502, 541, 583, 622, 664, 706, 748, 892
Bsu15I ATCGAT 1 cut(s) 1315
Bsu36I CCTNAGG 2 cut(s) 1005, 1260
BsuI GTATCC 1 cut(s) 1203
BsuRI GGCC 2 cut(s) 277, 828
BsuTUI ATCGAT 1 cut(s) 1315
BtgI CCRYGG 1 cut(s) 365
BtsCI GGATG 3 cut(s) 139, 249, 1351
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
CfoI GCGC 1 cut(s) 271
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 4 cut(s) 139, 276, 319, 788
Cfr42I CCGCGG 1 cut(s) 368
ClaI ATCGAT 1 cut(s) 1315
CspAI ACCGGT 1 cut(s) 157
CviAII CATG 1 cut(s) 955
DdeI CTNAG 6 cut(s) 393, 867, 1005, 1062, 1260, 1413
EaeI YGGCCR 1 cut(s) 826
EciI GGCGGA 2 cut(s) 122, 365
Eco130I CCWWGG 5 cut(s) 596, 677, 719, 761, 1216
Eco32I GATATC 2 cut(s) 822, 835
Eco47I GGWCC 3 cut(s) 139, 319, 788
Eco57I CTGAAG 3 cut(s) 315, 1077, 1218
Eco81I CCTNAGG 2 cut(s) 1005, 1260
Eco91I GGTNACC 1 cut(s) 1160
EcoNI CCTNNNNNAGG 1 cut(s) 1215
EcoO109I RGGNCCY 3 cut(s) 139, 319, 788
EcoO65I GGTNACC 1 cut(s) 1160
EcoRII CCWGG 2 cut(s) 397, 1330
EcoRV GATATC 2 cut(s) 822, 835
EcoT14I CCWWGG 5 cut(s) 596, 677, 719, 761, 1216
ErhI CCWWGG 5 cut(s) 596, 677, 719, 761, 1216
FaeI CATG 1 cut(s) 958
FaiI YATR 7 cut(s) 282, 290, 956, 1278, 1320, 1325, 1448
FaqI GGGAC 1 cut(s) 305
FatI CATG 1 cut(s) 954
FauI CCCGC 1 cut(s) 1041
Fnu4HI GCNGC 6 cut(s) 6, 272, 275, 431, 918, 1305
FokI GGATG 3 cut(s) 146, 256, 1358
Fsp4HI GCNGC 6 cut(s) 6, 272, 275, 431, 918, 1305
FspBI CTAG 4 cut(s) 51, 1067, 1110, 1217
GlaI GCGC 1 cut(s) 270
GluI GCNGC 6 cut(s) 6, 272, 275, 431, 918, 1305
HaeIII GGCC 2 cut(s) 277, 828
HapII CCGG 2 cut(s) 158, 785
HhaI GCGC 1 cut(s) 271
Hin1II CATG 1 cut(s) 958
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HinfI GANTC 3 cut(s) 47, 604, 1241
HpaII CCGG 2 cut(s) 158, 785
HphI GGTGA 9 cut(s) 172, 247, 351, 381, 467, 587, 668, 710, 1172
Hpy188I TCNGA 9 cut(s) 295, 327, 343, 421, 609, 901, 961, 982, 1416
Hpy188III TCNNGA 5 cut(s) 876, 1121, 1196, 1245, 1262
HpyAV CCTTC 6 cut(s) 329, 681, 906, 1081, 1135, 1231
HpyCH4IV ACGT 1 cut(s) 1081
HpyCH4V TGCA 4 cut(s) 620, 704, 1103, 1307
HpyF3I CTNAG 6 cut(s) 393, 867, 1005, 1062, 1260, 1413
HpySE526I ACGT 1 cut(s) 1081
Hsp92II CATG 1 cut(s) 958
HspAI GCGC 1 cut(s) 269
KspI CCGCGG 1 cut(s) 368
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 3 cut(s) 344, 489, 967
Lsp1109I GCAGC 2 cut(s) 929, 1291
LweI GCATC 3 cut(s) 274, 691, 1291
MaeI CTAG 4 cut(s) 51, 1067, 1110, 1217
MaeII ACGT 1 cut(s) 1081
MaeIII GTNAC 3 cut(s) 357, 1160, 1361
MflI RGATCY 9 cut(s) 463, 502, 541, 583, 622, 664, 706, 748, 892
MluCI AATT 3 cut(s) 165, 187, 1073
MlyI GAGTC 2 cut(s) 613, 1235
MmeI TCCRAC 3 cut(s) 587, 939, 999
MroXI GAANNNNTTC 2 cut(s) 406, 1203
MseI TTAA 5 cut(s) 24, 123, 263, 315, 1286
MslI CAYNNNNRTG 2 cut(s) 233, 1344
MspA1I CMGCKG 2 cut(s) 194, 367
MspI CCGG 2 cut(s) 158, 785
MspR9I CCNGG 3 cut(s) 399, 786, 1332
MvaI CCWGG 2 cut(s) 399, 1332
MvnI CGCG 2 cut(s) 271, 367
NciI CCSGG 1 cut(s) 786
NlaIII CATG 1 cut(s) 958
NlaIV GGNNCC 5 cut(s) 141, 321, 340, 789, 894
NmeAIII GCCGAG 2 cut(s) 459, 854
NmuCI GTSAC 2 cut(s) 357, 1160
NspI RCATGY 1 cut(s) 958
PciSI GCTCTTC 1 cut(s) 92
PdmI GAANNNNTTC 2 cut(s) 406, 1203
PfeI GAWTC 1 cut(s) 47
PflMI CCANNNNNTGG 1 cut(s) 812
PinAI ACCGGT 1 cut(s) 157
PkrI GCNGC 6 cut(s) 7, 273, 276, 432, 919, 1306
PleI GAGTC 2 cut(s) 612, 1235
PpsI GAGTC 2 cut(s) 612, 1235
PpuMI RGGWCCY 3 cut(s) 139, 319, 788
Psp5II RGGWCCY 3 cut(s) 139, 319, 788
Psp6I CCWGG 2 cut(s) 397, 1330
PspEI GGTNACC 1 cut(s) 1160
PspGI CCWGG 2 cut(s) 397, 1330
PspN4I GGNNCC 5 cut(s) 141, 321, 340, 789, 894
PspPI GGNCC 4 cut(s) 139, 276, 319, 788
PspPPI RGGWCCY 3 cut(s) 139, 319, 788
PsuI RGATCY 9 cut(s) 463, 502, 541, 583, 622, 664, 706, 748, 892
PvuII CAGCTG 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 233, 1344
SacII CCGCGG 1 cut(s) 368
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 5 cut(s) 24, 123, 263, 315, 1286
SatI GCNGC 6 cut(s) 6, 272, 275, 431, 918, 1305
Sau96I GGNCC 4 cut(s) 139, 276, 319, 788
SchI GAGTC 2 cut(s) 613, 1235
ScrFI CCNGG 3 cut(s) 399, 786, 1332
SfaNI GCATC 3 cut(s) 274, 691, 1291
Sfr303I CCGCGG 1 cut(s) 368
SgrBI CCGCGG 1 cut(s) 368
SinI GGWCC 3 cut(s) 139, 319, 788
SmiMI CAYNNNNRTG 2 cut(s) 233, 1344
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 3 cut(s) 165, 187, 1073
SspMI CTAG 4 cut(s) 51, 1067, 1110, 1217
StyD4I CCNGG 3 cut(s) 397, 784, 1330
StyI CCWWGG 5 cut(s) 596, 677, 719, 761, 1216
TaiI ACGT 1 cut(s) 1084
TaqI TCGA 3 cut(s) 410, 875, 1315
TasI AATT 3 cut(s) 165, 187, 1073
TauI GCSGC 4 cut(s) 8, 274, 277, 433
TfiI GAWTC 1 cut(s) 47
Tru1I TTAA 5 cut(s) 24, 123, 263, 315, 1286
Tru9I TTAA 5 cut(s) 24, 123, 263, 315, 1286
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 2 cut(s) 357, 1160
TseI GCWGC 2 cut(s) 917, 1304
Tsp45I GTSAC 2 cut(s) 357, 1160
TspDTI ATGAA 7 cut(s) 237, 260, 474, 759, 1011, 1154, 1250
TspGWI ACGGA 4 cut(s) 1120, 1380, 1405, 1433
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 812
VpaK11BI GGWCC 3 cut(s) 139, 319, 788
XagI CCTNNNNNAGG 1 cut(s) 1215
XceI RCATGY 1 cut(s) 958
XcmI CCANNNNNNNNNTGG 1 cut(s) 1338
XmaJI CCTAGG 1 cut(s) 1216
XmnI GAANNNNTTC 2 cut(s) 406, 1203
XspI CTAG 4 cut(s) 51, 1067, 1110, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.