Rorug04G0370100

BUD13 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010092
Physical Location & Seq
Forward (+)
54100875 .. 54102369
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0370100.1

Sequence Viewer

Length: 441 bp
ATGATTGTGATAGCGAAGGACGTTCTGGTAGTGTCACAGCTGGTGGGCATAGGAAGAGGCGCCAACCCGTTGCTTCAGCAGTGCAAACCCCTGGAGAACAGTGATATAATCTCAGACACCGCAAGACGCACATTGGCAGCAGCATCAGCAGCGCCTCACCTGAAAAGTAGAAGGAAGGAAGAATCAAATCCTGAATCTGTTGGTGTAGACCTGATTCAGGTCACAACATTAAAAACTGTGGAGTCAACAGAGGAGCGAGTTGTGAGGTTTGCAACACCAGAACCACAAGACACTGTTAATGGCAATGCTGATGCTGATGCAACGAAGTTGGTTGAAGAGAGAGAGTTGAGTACGGAAGTGAATGGCACAGAATCTAGTGGTGGCAACAGCACAGGTGGGGAGAGTGACAACAGCACAGGTGATGAGAGCGGCGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bud13

Protein Analysis

146

Amino Acids

15.36

Weight (kDa)

4.46

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccBSI CCGCTC 1 cut(s) 429
AccI GTMKAC 1 cut(s) 207
AciI CCGC 2 cut(s) 120, 429
AcuI CTGAAG 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 1 cut(s) 335
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 1 cut(s) 40
AluI AGCT 1 cut(s) 40
ApeKI GCWGC 3 cut(s) 137, 140, 149
AspLEI GCGC 2 cut(s) 62, 154
AsuHPI GGTGA 2 cut(s) 149, 431
BanI GGYRCC 1 cut(s) 59
BbvI GCAGC 3 cut(s) 149, 152, 161
BciT130I CCWGG 1 cut(s) 92
BfaI CTAG 1 cut(s) 375
BfoI RGCGCY 2 cut(s) 63, 155
BisI GCNGC 4 cut(s) 138, 141, 150, 430
BlsI GCNGC 4 cut(s) 139, 142, 151, 431
Bme1390I CCNGG 1 cut(s) 92
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 92
BmsI GCATC 3 cut(s) 152, 301, 307
BpmI CTGGAG 1 cut(s) 113
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse3DI GCAATG 1 cut(s) 310
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 90
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 266
BseXI GCAGC 3 cut(s) 149, 152, 161
BshNI GGYRCC 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 217
BspACI CCGC 2 cut(s) 120, 429
BspCNI CTCAG 1 cut(s) 125
BspLI GGNNCC 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 310
BssECI CCNNGG 1 cut(s) 90
BssNI GRCGYC 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 3 cut(s) 101, 238, 295
Bst6I CTCTTC 2 cut(s) 49, 330
BstACI GRCGYC 1 cut(s) 60
BstDEI CTNAG 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 215
BstH2I RGCGCY 2 cut(s) 63, 155
BstHHI GCGC 2 cut(s) 62, 154
BstMWI GCNNNNNNNGC 2 cut(s) 146, 149
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 149, 152, 161
BtsI GCAGTG 1 cut(s) 86
BtsIMutI CAGTG 3 cut(s) 86, 106, 291
CfoI GCGC 2 cut(s) 62, 154
CseI GACGC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 351
CviJI RGCY 1 cut(s) 40
CviKI_1 RGCY 1 cut(s) 40
CviQI GTAC 1 cut(s) 351
DdeI CTNAG 1 cut(s) 112
DinI GGCGCC 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 49, 330
EarI CTCTTC 2 cut(s) 49, 330
Eco57I CTGAAG 1 cut(s) 59
EcoNI CCTNNNNNAGG 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 90
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
FaiI YATR 2 cut(s) 50, 107
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 4 cut(s) 138, 141, 150, 430
Fsp4HI GCNGC 4 cut(s) 138, 141, 150, 430
FspBI CTAG 1 cut(s) 375
GlaI GCGC 2 cut(s) 61, 153
GluI GCNGC 4 cut(s) 138, 141, 150, 430
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 2 cut(s) 63, 155
HgaI GACGC 1 cut(s) 135
HhaI GCGC 2 cut(s) 62, 154
Hin1I GRCGYC 1 cut(s) 60
Hin6I GCGC 2 cut(s) 60, 152
HinP1I GCGC 2 cut(s) 60, 152
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HinfI GANTC 5 cut(s) 182, 194, 214, 242, 371
HphI GGTGA 2 cut(s) 149, 431
Hpy166II GTNNAC 2 cut(s) 208, 246
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 2 cut(s) 208, 246
HpyAV CCTTC 3 cut(s) 10, 165, 169
HpyCH4III ACNGT 3 cut(s) 101, 238, 295
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 3 cut(s) 84, 272, 320
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 149
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 1 cut(s) 60
HspAI GCGC 2 cut(s) 60, 152
KasI GGCGCC 1 cut(s) 59
LmnI GCTCC 1 cut(s) 253
Lsp1109I GCAGC 3 cut(s) 149, 152, 161
LweI GCATC 3 cut(s) 152, 301, 307
MaeI CTAG 1 cut(s) 375
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 3 cut(s) 33, 220, 404
MbiI CCGCTC 1 cut(s) 429
MboII GAAGA 3 cut(s) 66, 191, 347
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 4 cut(s) 50, 165, 244, 258
MseI TTAA 2 cut(s) 230, 297
MspA1I CMGCKG 1 cut(s) 40
MspR9I CCNGG 1 cut(s) 92
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 2 cut(s) 146, 149
NarI GGCGCC 1 cut(s) 60
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 3 cut(s) 33, 220, 404
PfeI GAWTC 4 cut(s) 182, 194, 214, 371
PkrI GCNGC 4 cut(s) 139, 142, 151, 431
PleI GAGTC 1 cut(s) 250
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 40
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
SaqAI TTAA 2 cut(s) 230, 297
SatI GCNGC 4 cut(s) 138, 141, 150, 430
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 92
SetI ASST 8 cut(s) 24, 42, 162, 213, 222, 269, 397, 421
SfaNI GCATC 3 cut(s) 152, 301, 307
SfoI GGCGCC 1 cut(s) 61
SsiI CCGC 2 cut(s) 120, 429
SspDI GGCGCC 1 cut(s) 59
SspMI CTAG 1 cut(s) 375
StyD4I CCNGG 1 cut(s) 90
TaaI ACNGT 3 cut(s) 101, 238, 295
TaiI ACGT 1 cut(s) 24
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 4 cut(s) 182, 194, 214, 371
Tru1I TTAA 2 cut(s) 230, 297
Tru9I TTAA 2 cut(s) 230, 297
TscAI CASTG 3 cut(s) 86, 106, 298
TseFI GTSAC 3 cut(s) 33, 220, 404
TseI GCWGC 3 cut(s) 137, 140, 149
Tsp45I GTSAC 3 cut(s) 33, 220, 404
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 3 cut(s) 86, 106, 298
XagI CCTNNNNNAGG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 207
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.