FvH4_5g03820

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007700
Physical Location & Seq
Reverse (-)
2285329 .. 2288215
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03820.t1

Sequence Viewer

Length: 693 bp
ATGAACGACACCGATGTCTCCAAGCAGATCCAGCAGATGGTGAGGTTCATTCGCCAGGAAGCTGAGGAAAAGGCCAACGAGATCTCCGTCTCCGCCGAAGAAGAATTCAACATTGAGAAGCTGCAGCTGGTTGAGGCGGAGAAGAAGAAGATCAGGCAGGAGTATGAGAAGAAGGAGAAGCAAGTCGATGTGCGAAAGAAGATTGAGTATTCCATGCAACTCAATGCTTCTCGTATTAAGGTTCTTCAAGCTCAAGATGATGTGGTCAACTCCATGAAGGAGGCTGCATCCAAGGAGCTTCTGAATGTGAGCAAAGATCATAAAAAATATGCAAAGCTCCTGAAAGATCTGATCATTCAGAATTTGCTTAGGCTGAAAGAGCCTGCTGTGCTGTTGCGTTGTCGCAAGGATGACGTGCATCTAGTGGAGTCTATTCTGGAAGCAGCTGCGCAGGAATATGCAGAGAAAAGAGATGTTCATGCACCAGAGATCCTAGTTGATCACACTGTCTATCTTCCACCTGCTCCCACCCATCATAATCCCCATGTTAATTCCTGTTCTGGAGGTGTAGTCTTGGCTTCTCGAGACGGGAAGATTGTGTGTGAGAACACTCTTGATGCACGGTTGGATGTTGTATTCCGTAAAAAGCTTCCAGAGATCCGCAGAAGGCTCTTTGGTCAGATTGTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
vATP-synt_E

Protein Analysis

231

Amino Acids

26.46

Weight (kDa)

8.3

Isoelectric Point (pI)

50.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
vATP-synt_E PF01991 16 - 225 3.4e-70 ATP synthase (E/31 kDa) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 529
AasI GACNNNNNNGTC 1 cut(s) 14
Acc16I TGCGCA 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 529
AccB7I CCANNNNNTGG 1 cut(s) 37
AciI CCGC 3 cut(s) 93, 137, 661
AclWI GGATC 3 cut(s) 22, 484, 652
AcsI RAATTY 2 cut(s) 104, 361
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 2 cut(s) 109, 248
AjiI CACGTC 1 cut(s) 415
AjnI CCWGG 1 cut(s) 54
AluBI AGCT 8 cut(s) 62, 121, 127, 251, 298, 337, 446, 649
AluI AGCT 8 cut(s) 62, 121, 127, 251, 298, 337, 446, 649
Alw26I GTCTC 3 cut(s) 22, 94, 579
AlwI GGATC 3 cut(s) 22, 484, 652
Ama87I CYCGRG 1 cut(s) 582
AoxI GGCC 1 cut(s) 72
ApeKI GCWGC 5 cut(s) 121, 124, 284, 443, 446
ApoI RAATTY 2 cut(s) 104, 361
AspLEI GCGC 1 cut(s) 451
AsuHPI GGTGA 1 cut(s) 52
AvaI CYCGRG 1 cut(s) 582
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 5 cut(s) 108, 136, 271, 433, 455
BccI CCATC 2 cut(s) 31, 540
BciT130I CCWGG 1 cut(s) 56
BclI TGATCA 2 cut(s) 351, 499
BcoDI GTCTC 3 cut(s) 22, 94, 579
BfaI CTAG 2 cut(s) 422, 494
BfmI CTRYAG 1 cut(s) 122
BfuAI ACCTGC 1 cut(s) 529
BglII AGATCT 2 cut(s) 81, 346
BisI GCNGC 5 cut(s) 122, 125, 285, 444, 447
BlsI GCNGC 5 cut(s) 123, 126, 286, 445, 448
Bme1390I CCNGG 1 cut(s) 56
BmeT110I CYCGRG 1 cut(s) 582
BmgBI CACGTC 1 cut(s) 415
BmrFI CCNGG 1 cut(s) 56
BmsI GCATC 3 cut(s) 296, 427, 607
BpmI CTGGAG 1 cut(s) 582
Bpu10I CCTNAGC 2 cut(s) 63, 368
BpuEI CTTGAG 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 291
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 555, 585
Bsc4I CCNNNNNNNGG 1 cut(s) 37
BseBI CCWGG 1 cut(s) 56
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 3 cut(s) 287, 415, 634
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMII CTCAG 1 cut(s) 54
BseXI GCAGC 5 cut(s) 108, 136, 271, 433, 455
BshFI GGCC 1 cut(s) 74
BsiHKCI CYCGRG 1 cut(s) 582
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 3 cut(s) 22, 94, 579
BsmBI CGTCTC 2 cut(s) 94, 579
BsnI GGCC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 582
Bsp143I GATC 9 cut(s) 27, 81, 150, 316, 346, 351, 489, 499, 657
BspACI CCGC 3 cut(s) 93, 137, 661
BspANI GGCC 1 cut(s) 74
BspCNI CTCAG 1 cut(s) 55
BspMAI CTGCAG 1 cut(s) 126
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 3 cut(s) 22, 484, 652
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 9 cut(s) 27, 81, 150, 316, 346, 351, 489, 499, 657
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 56
Bst4CI ACNGT 2 cut(s) 508, 624
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 2 cut(s) 63, 368
BstF5I GGATG 3 cut(s) 287, 415, 634
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 9 cut(s) 30, 84, 153, 319, 349, 354, 492, 502, 660
BstMAI GTCTC 3 cut(s) 22, 94, 579
BstMBI GATC 9 cut(s) 27, 81, 150, 316, 346, 351, 489, 499, 657
BstMWI GCNNNNNNNGC 3 cut(s) 31, 379, 388
BstNI CCWGG 1 cut(s) 56
BstSCI CCNGG 1 cut(s) 54
BstSFI CTRYAG 1 cut(s) 122
BstV1I GCAGC 5 cut(s) 108, 136, 271, 433, 455
BstX2I RGATCY 5 cut(s) 27, 81, 346, 489, 657
BstYI RGATCY 5 cut(s) 27, 81, 346, 489, 657
BsuRI GGCC 1 cut(s) 74
BtrI CACGTC 1 cut(s) 415
BtsCI GGATG 3 cut(s) 287, 415, 634
BtsIMutI CAGTG 1 cut(s) 504
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 1 cut(s) 384
CfoI GCGC 1 cut(s) 451
CviAII CATG 4 cut(s) 214, 274, 479, 545
DdeI CTNAG 2 cut(s) 63, 368
DpnI GATC 9 cut(s) 29, 83, 152, 318, 348, 353, 491, 501, 659
DpnII GATC 9 cut(s) 27, 81, 150, 316, 346, 351, 489, 499, 657
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
EciI GGCGGA 2 cut(s) 82, 152
Eco130I CCWWGG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 582
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 54
EcoT14I CCWWGG 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 291
Esp3I CGTCTC 2 cut(s) 94, 579
FaeI CATG 4 cut(s) 217, 277, 482, 548
FatI CATG 4 cut(s) 213, 273, 478, 544
FbaI TGATCA 2 cut(s) 351, 499
Fnu4HI GCNGC 5 cut(s) 122, 125, 285, 444, 447
FokI GGATG 3 cut(s) 274, 422, 641
Fsp4HI GCNGC 5 cut(s) 122, 125, 285, 444, 447
FspBI CTAG 2 cut(s) 422, 494
FspI TGCGCA 1 cut(s) 450
GlaI GCGC 1 cut(s) 450
GluI GCNGC 5 cut(s) 122, 125, 285, 444, 447
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 1 cut(s) 74
HhaI GCGC 1 cut(s) 451
Hin1II CATG 4 cut(s) 217, 277, 482, 548
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 1 cut(s) 428
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 4 cut(s) 303, 351, 360, 681
Hpy188III TCNNGA 8 cut(s) 254, 340, 437, 561, 582, 584, 614, 653
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 3 cut(s) 166, 271, 660
HpyCH4III ACNGT 2 cut(s) 508, 624
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 9 cut(s) 124, 217, 287, 332, 418, 461, 482, 620, 689
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 379, 388
HpyF3I CTNAG 2 cut(s) 63, 368
HpySE526I ACGT 1 cut(s) 414
Hsp92II CATG 4 cut(s) 217, 277, 482, 548
HspAI GCGC 1 cut(s) 449
Ksp22I TGATCA 2 cut(s) 351, 499
Kzo9I GATC 9 cut(s) 27, 81, 150, 316, 346, 351, 489, 499, 657
LmnI GCTCC 3 cut(s) 295, 342, 529
Lsp1109I GCAGC 5 cut(s) 108, 136, 271, 433, 455
LweI GCATC 3 cut(s) 296, 427, 607
MaeI CTAG 2 cut(s) 422, 494
MaeII ACGT 1 cut(s) 414
MalI GATC 9 cut(s) 29, 83, 152, 318, 348, 353, 491, 501, 659
MboI GATC 9 cut(s) 27, 81, 150, 316, 346, 351, 489, 499, 657
MflI RGATCY 5 cut(s) 27, 81, 346, 489, 657
MluCI AATT 3 cut(s) 104, 361, 550
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 1 cut(s) 606
MnlI CCTC 5 cut(s) 36, 58, 127, 274, 557
MseI TTAA 2 cut(s) 237, 549
MspA1I CMGCKG 2 cut(s) 127, 446
MspR9I CCNGG 1 cut(s) 56
MvaI CCWGG 1 cut(s) 56
MwoI GCNNNNNNNGC 3 cut(s) 31, 379, 388
NdeII GATC 9 cut(s) 27, 81, 150, 316, 346, 351, 489, 499, 657
NlaIII CATG 4 cut(s) 217, 277, 482, 548
NsbI TGCGCA 1 cut(s) 450
PaeR7I CTCGAG 1 cut(s) 582
PaqCI CACCTGC 1 cut(s) 529
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PflMI CCANNNNNTGG 1 cut(s) 37
PkrI GCNGC 5 cut(s) 123, 126, 286, 445, 448
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
Psp6I CCWGG 1 cut(s) 54
PspGI CCWGG 1 cut(s) 54
PstI CTGCAG 1 cut(s) 126
PsuI RGATCY 5 cut(s) 27, 81, 346, 489, 657
PvuII CAGCTG 2 cut(s) 127, 446
SaqAI TTAA 2 cut(s) 237, 549
SatI GCNGC 5 cut(s) 122, 125, 285, 444, 447
Sau3AI GATC 9 cut(s) 27, 81, 150, 316, 346, 351, 489, 499, 657
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 1 cut(s) 56
SfaNI GCATC 3 cut(s) 296, 427, 607
SfcI CTRYAG 1 cut(s) 122
Sfr274I CTCGAG 1 cut(s) 582
SlaI CTCGAG 1 cut(s) 582
SmlI CTYRAG 2 cut(s) 252, 582
SmoI CTYRAG 2 cut(s) 252, 582
Sse9I AATT 3 cut(s) 104, 361, 550
SsiI CCGC 3 cut(s) 93, 137, 661
SspMI CTAG 2 cut(s) 422, 494
StyD4I CCNGG 1 cut(s) 54
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 2 cut(s) 508, 624
TaiI ACGT 1 cut(s) 417
TaqI TCGA 2 cut(s) 186, 583
TasI AATT 3 cut(s) 104, 361, 550
Tru1I TTAA 2 cut(s) 237, 549
Tru9I TTAA 2 cut(s) 237, 549
TscAI CASTG 1 cut(s) 511
TseI GCWGC 5 cut(s) 121, 124, 284, 443, 446
TspDTI ATGAA 4 cut(s) 17, 37, 290, 467
TspGWI ACGGA 2 cut(s) 76, 629
TspRI CASTG 1 cut(s) 511
Van91I CCANNNNNTGG 1 cut(s) 37
XapI RAATTY 2 cut(s) 104, 361
XhoI CTCGAG 1 cut(s) 582
XspI CTAG 2 cut(s) 422, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.