Rh7DG154300

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0007700
Physical Location & Seq
Forward (+)
13249046 .. 13251132
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG154300.1

Sequence Viewer

Length: 606 bp
ATGAACGACGCCGATGTCTCGAAGCAGATCCAGCAGATGGTGAGGTTCATTCGCCAAGAAGCCGAGGAAAAGGCCAACGAGATCTCCGTCTCCGCCGAAGAAGTTGTGTGGAGTAGTGAGTATTCCATGCAGCTCAATGCTTCTCGTATTAAAGTTCTTCAAGCTCAAGATGATGTAGTCAATGCCATGAAGGATGCTGCATCCAAGGAGCTCCTGAATGTGAGCAAAGATCACCATGGGTATGCAAAGCTTCTGAAAGATCTTATCATTCAGAGTTTGCTCAGGCTGAAAGAGCCTGCTGTTTTGTTGCGTTGTCGCAAAGATGACGTGCATCTGGTGGAGTCTGTTCTGGAAGCAGCAGCAAAGGAATATGCGGAGAAAAGAGATGTTCATGCACCAGAGATTTTAGTTGACCATACTGTCTATCTTCCACCTGCTCCCACCCATTATAATCCCCATGTTAATTCCTGTTCTGGAGGTGTAGTCTTGGCTTCTCGAGATGGGAAGATCGTGTGTGAGAACACTCTCGATGCACGGTTGGATGTTGTATTCCGTAAAAAACTTCCTGAGATCCGCAGAAGGCTCTTCGGTCAGGTTGTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
vATP-synt_E

Protein Analysis

201

Amino Acids

22.63

Weight (kDa)

6.39

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
vATP-synt_E PF01991 39 - 196 5.3e-46 ATP synthase (E/31 kDa) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
AarI CACCTGC 1 cut(s) 442
AasI GACNNNNNNGTC 2 cut(s) 14, 419
Acc36I ACCTGC 1 cut(s) 442
AccB7I CCANNNNNTGG 1 cut(s) 37
AciI CCGC 3 cut(s) 93, 374, 574
AclWI GGATC 2 cut(s) 22, 565
AcyI GRCGYC 1 cut(s) 9
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 1 cut(s) 161
AjiI CACGTC 1 cut(s) 328
AluBI AGCT 4 cut(s) 133, 164, 211, 250
AluI AGCT 4 cut(s) 133, 164, 211, 250
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 2 cut(s) 22, 94
AlwI GGATC 2 cut(s) 22, 565
Ama87I CYCGRG 1 cut(s) 495
AoxI GGCC 1 cut(s) 72
ApeKI GCWGC 4 cut(s) 130, 197, 356, 359
AsuHPI GGTGA 2 cut(s) 52, 224
AvaI CYCGRG 1 cut(s) 495
BanII GRGCYC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 4 cut(s) 142, 184, 368, 371
BccI CCATC 2 cut(s) 31, 494
BcoDI GTCTC 2 cut(s) 22, 94
BfuAI ACCTGC 1 cut(s) 442
BglII AGATCT 2 cut(s) 81, 259
BisI GCNGC 4 cut(s) 131, 198, 357, 360
BlsI GCNGC 4 cut(s) 132, 199, 358, 361
BmeT110I CYCGRG 1 cut(s) 495
BmgBI CACGTC 1 cut(s) 328
BmsI GCATC 4 cut(s) 184, 209, 340, 520
BpmI CTGGAG 1 cut(s) 495
Bpu10I CCTNAGC 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 3 cut(s) 63, 204, 235
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 468, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 37
BseDI CCNNGG 3 cut(s) 63, 204, 235
BseGI GGATG 3 cut(s) 199, 200, 547
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMII CTCAG 2 cut(s) 295, 558
BseXI GCAGC 4 cut(s) 142, 184, 368, 371
BshFI GGCC 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 495
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 2 cut(s) 22, 94
BsmBI CGTCTC 1 cut(s) 94
BsnI GGCC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 495
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 6 cut(s) 27, 81, 229, 259, 507, 570
Bsp19I CCATGG 1 cut(s) 235
BspACI CCGC 3 cut(s) 93, 374, 574
BspANI GGCC 1 cut(s) 74
BspCNI CTCAG 2 cut(s) 294, 559
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 2 cut(s) 22, 565
BspQI GCTCTTC 1 cut(s) 590
BssECI CCNNGG 3 cut(s) 63, 204, 235
BssMI GATC 6 cut(s) 27, 81, 229, 259, 507, 570
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 2 cut(s) 204, 235
Bst4CI ACNGT 2 cut(s) 421, 537
Bst6I CTCTTC 1 cut(s) 590
BstACI GRCGYC 1 cut(s) 9
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 2 cut(s) 281, 567
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 3 cut(s) 199, 200, 547
BstKTI GATC 6 cut(s) 30, 84, 232, 262, 510, 573
BstMAI GTCTC 2 cut(s) 22, 94
BstMBI GATC 6 cut(s) 27, 81, 229, 259, 507, 570
BstMWI GCNNNNNNNGC 2 cut(s) 31, 292
BstV1I GCAGC 4 cut(s) 142, 184, 368, 371
BstX2I RGATCY 4 cut(s) 27, 81, 259, 570
BstYI RGATCY 4 cut(s) 27, 81, 259, 570
BsuRI GGCC 1 cut(s) 74
BtgI CCRYGG 1 cut(s) 235
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 3 cut(s) 199, 200, 547
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 297
CseI GACGC 1 cut(s) 17
CviAII CATG 5 cut(s) 127, 187, 236, 392, 458
DdeI CTNAG 2 cut(s) 281, 567
DpnI GATC 6 cut(s) 29, 83, 231, 261, 509, 572
DpnII GATC 6 cut(s) 27, 81, 229, 259, 507, 570
DrdI GACNNNNNNGTC 2 cut(s) 14, 419
DseDI GACNNNNNNGTC 2 cut(s) 14, 419
Eam1104I CTCTTC 1 cut(s) 590
EarI CTCTTC 1 cut(s) 590
EciI GGCGGA 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 2 cut(s) 204, 235
Eco24I GRGCYC 1 cut(s) 213
Eco53kI GAGCTC 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 495
EcoICRI GAGCTC 1 cut(s) 211
EcoT14I CCWWGG 2 cut(s) 204, 235
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 2 cut(s) 204, 235
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 5 cut(s) 130, 190, 239, 395, 461
FatI CATG 5 cut(s) 126, 186, 235, 391, 457
Fnu4HI GCNGC 4 cut(s) 131, 198, 357, 360
FokI GGATG 3 cut(s) 187, 206, 554
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 4 cut(s) 131, 198, 357, 360
GluI GCNGC 4 cut(s) 131, 198, 357, 360
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 1 cut(s) 74
HgaI GACGC 1 cut(s) 17
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 5 cut(s) 130, 190, 239, 395, 461
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 1 cut(s) 341
HphI GGTGA 2 cut(s) 52, 224
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 2 cut(s) 255, 273
Hpy188III TCNNGA 9 cut(s) 19, 167, 214, 350, 474, 495, 497, 527, 566
Hpy8I GTNNAC 1 cut(s) 412
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 2 cut(s) 184, 573
HpyCH4III ACNGT 2 cut(s) 421, 537
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 7 cut(s) 130, 200, 245, 331, 395, 533, 602
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 292
HpyF3I CTNAG 2 cut(s) 281, 567
HpySE526I ACGT 1 cut(s) 327
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 5 cut(s) 130, 190, 239, 395, 461
Kzo9I GATC 6 cut(s) 27, 81, 229, 259, 507, 570
LguI GCTCTTC 1 cut(s) 590
LmnI GCTCC 3 cut(s) 208, 216, 442
Lsp1109I GCAGC 4 cut(s) 142, 184, 368, 371
LweI GCATC 4 cut(s) 184, 209, 340, 520
MaeII ACGT 1 cut(s) 327
MalI GATC 6 cut(s) 29, 83, 231, 261, 509, 572
MboI GATC 6 cut(s) 27, 81, 229, 259, 507, 570
MboII GAAGA 5 cut(s) 110, 149, 419, 517, 577
MflI RGATCY 4 cut(s) 27, 81, 259, 570
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 1 cut(s) 463
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 1 cut(s) 519
MnlI CCTC 3 cut(s) 36, 58, 470
MseI TTAA 2 cut(s) 150, 462
MslI CAYNNNNRTG 1 cut(s) 240
MwoI GCNNNNNNNGC 2 cut(s) 31, 292
NcoI CCATGG 1 cut(s) 235
NdeII GATC 6 cut(s) 27, 81, 229, 259, 507, 570
NlaIII CATG 5 cut(s) 130, 190, 239, 395, 461
NmeAIII GCCGAG 1 cut(s) 88
PaeR7I CTCGAG 1 cut(s) 495
PaqCI CACCTGC 1 cut(s) 442
PciSI GCTCTTC 1 cut(s) 590
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PflMI CCANNNNNTGG 1 cut(s) 37
PkrI GCNGC 4 cut(s) 132, 199, 358, 361
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
PsiI TTATAA 1 cut(s) 450
Psp124BI GAGCTC 1 cut(s) 213
PsuI RGATCY 4 cut(s) 27, 81, 259, 570
RseI CAYNNNNRTG 1 cut(s) 240
SacI GAGCTC 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 590
SaqAI TTAA 2 cut(s) 150, 462
SatI GCNGC 4 cut(s) 131, 198, 357, 360
Sau3AI GATC 6 cut(s) 27, 81, 229, 259, 507, 570
SchI GAGTC 1 cut(s) 350
SduI GDGCHC 1 cut(s) 213
SetI ASST 9 cut(s) 47, 135, 166, 213, 252, 330, 436, 481, 597
SfaNI GCATC 4 cut(s) 184, 209, 340, 520
Sfr274I CTCGAG 1 cut(s) 495
SlaI CTCGAG 1 cut(s) 495
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 2 cut(s) 165, 495
SmoI CTYRAG 2 cut(s) 165, 495
Sse9I AATT 1 cut(s) 463
SsiI CCGC 3 cut(s) 93, 374, 574
SstI GAGCTC 1 cut(s) 213
StyI CCWWGG 2 cut(s) 204, 235
TaaI ACNGT 2 cut(s) 421, 537
TaiI ACGT 1 cut(s) 330
TaqI TCGA 3 cut(s) 20, 496, 528
TaqII GACCGA 1 cut(s) 578
TasI AATT 1 cut(s) 463
Tru1I TTAA 2 cut(s) 150, 462
Tru9I TTAA 2 cut(s) 150, 462
TseI GCWGC 4 cut(s) 130, 197, 356, 359
TspDTI ATGAA 4 cut(s) 17, 37, 203, 380
TspGWI ACGGA 2 cut(s) 76, 542
Van91I CCANNNNNTGG 1 cut(s) 37
XhoI CTCGAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.