MD14G1119200.v1.1

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007700
Physical Location & Seq
Reverse (-)
19346298 .. 19348806
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1119200.v1.1.491

Sequence Viewer

Length: 693 bp
ATGAACGACGCAGATGTCTCCAAGCAGATCCAGCAGATGGTGAGGTTCATCCGCCAAGAAGCTGAGGAGAAGGCCAACGAGATCTCCGTATCTGCTGAGGAAGAATTCAACATTGAGAAGTTGCAGCTCGTCGAGGCGGAGAAGAAGAAGATCAGGCAAGAGTATGAGAAGAAGGAGAAGCAAGTCGACGTCCGAAAGAAGATTGAATACTCGATGCAGCTCAATGCTTCTCGTATTAAAGTTCTTCAGGCTCAGGATGACGTGGTTAACTCCATGAAGGAGGCAGCATCCAACGAGCTTCTGAATGTGAGCCAAGATCACCATGCGTACAAAAAGCTTCTTAAAGATCTCATCGTTCAGAGTTTGCTCAGGCTGAAAGAGCCTGCGGTTTTGTTGCGTTGTCGGAAAGATGACCTACATCTGGTGGAGGCTGTACTGGAATCAGCGGGAGAGGAATATGCAGGCAAAGCAAAAGTTCATTCACCAGAGATTATAGTGGACACTACTGTCTTTCTTCCACCTGCTCCTACCCATCATAATCCCCATGTTTCTTCCTGTTCTGGAGGTGTAGTCCTGGCTTCTCGAGATGGGAAGATTGTGTGTGAGAACACGCTTGATGCACGATTGGATGTTGTATTCCGTAAAAAACTTCCAGAGATCCGCAGAAAGCTCTTTGGTCAGGTTGCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
vATP-synt_E

Protein Analysis

231

Amino Acids

26.09

Weight (kDa)

7.06

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
vATP-synt_E PF01991 16 - 225 4.5e-72 ATP synthase (E/31 kDa) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 529
AasI GACNNNNNNGTC 2 cut(s) 14, 506
AatII GACGTC 1 cut(s) 192
Acc36I ACCTGC 1 cut(s) 529
AccB7I CCANNNNNTGG 1 cut(s) 37
AccI GTMKAC 1 cut(s) 186
AciI CCGC 5 cut(s) 52, 137, 386, 446, 661
AclWI GGATC 2 cut(s) 22, 652
AcsI RAATTY 1 cut(s) 104
AcuI CTGAAG 1 cut(s) 230
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 2 cut(s) 329, 435
AfiI CCNNNNNNNGG 2 cut(s) 37, 421
AgsI TTSAA 2 cut(s) 109, 206
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 1 cut(s) 573
AluBI AGCT 6 cut(s) 62, 127, 220, 298, 337, 670
AluI AGCT 6 cut(s) 62, 127, 220, 298, 337, 670
Alw26I GTCTC 1 cut(s) 22
AlwI GGATC 2 cut(s) 22, 652
Ama87I CYCGRG 1 cut(s) 582
AoxI GGCC 1 cut(s) 72
ApeKI GCWGC 4 cut(s) 124, 217, 284, 686
ApoI RAATTY 1 cut(s) 104
AsuHPI GGTGA 3 cut(s) 52, 311, 474
AvaI CYCGRG 1 cut(s) 582
BarI GAAGNNNNNNTAC 2 cut(s) 191, 223
BbvCI CCTCAGC 2 cut(s) 63, 96
BbvI GCAGC 4 cut(s) 136, 229, 296, 673
BccI CCATC 3 cut(s) 31, 540, 581
BciT130I CCWGG 1 cut(s) 575
BcoDI GTCTC 1 cut(s) 22
BfuAI ACCTGC 1 cut(s) 529
BglII AGATCT 2 cut(s) 81, 346
BisI GCNGC 4 cut(s) 125, 218, 285, 687
BlsI GCNGC 4 cut(s) 126, 219, 286, 688
Bme1390I CCNGG 1 cut(s) 575
BmeT110I CYCGRG 1 cut(s) 582
BmgBI CACGTC 1 cut(s) 262
BmrFI CCNGG 1 cut(s) 575
BmsI GCATC 3 cut(s) 204, 296, 607
BpmI CTGGAG 1 cut(s) 582
Bpu10I CCTNAGC 4 cut(s) 63, 96, 252, 368
BsaHI GRCGYC 1 cut(s) 189
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 555, 585
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 421
Bse1I ACTGG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 575
BseGI GGATG 4 cut(s) 48, 262, 287, 634
BseLI CCNNNNNNNGG 2 cut(s) 37, 421
BseMII CTCAG 4 cut(s) 54, 87, 266, 382
BseNI ACTGG 1 cut(s) 441
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 4 cut(s) 136, 229, 296, 673
BshFI GGCC 1 cut(s) 74
BsiHKCI CYCGRG 1 cut(s) 582
BslI CCNNNNNNNGG 2 cut(s) 37, 421
BsmAI GTCTC 1 cut(s) 22
BsnI GGCC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 582
Bsp143I GATC 6 cut(s) 27, 81, 150, 316, 346, 657
BspACI CCGC 5 cut(s) 52, 137, 386, 446, 661
BspANI GGCC 1 cut(s) 74
BspCNI CTCAG 4 cut(s) 55, 88, 265, 381
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 2 cut(s) 22, 652
BsrI ACTGG 1 cut(s) 441
BssMI GATC 6 cut(s) 27, 81, 150, 316, 346, 657
BssNI GRCGYC 1 cut(s) 189
Bst2UI CCWGG 1 cut(s) 575
Bst4CI ACNGT 1 cut(s) 508
BstACI GRCGYC 1 cut(s) 189
BstC8I GCNNGC 2 cut(s) 384, 463
BstDEI CTNAG 4 cut(s) 63, 96, 252, 368
BstF5I GGATG 4 cut(s) 48, 262, 287, 634
BstKTI GATC 6 cut(s) 30, 84, 153, 319, 349, 660
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 6 cut(s) 27, 81, 150, 316, 346, 657
BstMWI GCNNNNNNNGC 3 cut(s) 31, 379, 467
BstNI CCWGG 1 cut(s) 575
BstSCI CCNGG 1 cut(s) 573
BstV1I GCAGC 4 cut(s) 136, 229, 296, 673
BstX2I RGATCY 4 cut(s) 27, 81, 346, 657
BstYI RGATCY 4 cut(s) 27, 81, 346, 657
BsuRI GGCC 1 cut(s) 74
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 4 cut(s) 48, 262, 287, 634
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 2 cut(s) 384, 463
CseI GACGC 1 cut(s) 17
Csp6I GTAC 2 cut(s) 328, 434
CviAII CATG 3 cut(s) 274, 323, 545
CviQI GTAC 2 cut(s) 328, 434
DdeI CTNAG 4 cut(s) 63, 96, 252, 368
DpnI GATC 6 cut(s) 29, 83, 152, 318, 348, 659
DpnII GATC 6 cut(s) 27, 81, 150, 316, 346, 657
DrdI GACNNNNNNGTC 2 cut(s) 14, 506
DseDI GACNNNNNNGTC 2 cut(s) 14, 506
EciI GGCGGA 2 cut(s) 41, 152
Eco57I CTGAAG 1 cut(s) 230
Eco88I CYCGRG 1 cut(s) 582
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 573
FaeI CATG 3 cut(s) 277, 326, 548
FaiI YATR 8 cut(s) 165, 275, 324, 459, 494, 537, 546, 691
FatI CATG 3 cut(s) 273, 322, 544
FauI CCCGC 1 cut(s) 439
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 4 cut(s) 125, 218, 285, 687
FokI GGATG 4 cut(s) 35, 269, 274, 641
Fsp4HI GCNGC 4 cut(s) 125, 218, 285, 687
GluI GCNGC 4 cut(s) 125, 218, 285, 687
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 1 cut(s) 74
HgaI GACGC 1 cut(s) 17
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 3 cut(s) 277, 326, 548
HincII GTYRAC 2 cut(s) 187, 268
HindII GTYRAC 2 cut(s) 187, 268
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 1 cut(s) 440
HpaI GTTAAC 1 cut(s) 268
HphI GGTGA 3 cut(s) 52, 311, 474
Hpy166II GTNNAC 3 cut(s) 187, 268, 499
Hpy188I TCNGA 4 cut(s) 194, 303, 360, 405
Hpy188III TCNNGA 5 cut(s) 254, 561, 582, 584, 653
Hpy8I GTNNAC 3 cut(s) 187, 268, 499
Hpy99I CGWCG 3 cut(s) 11, 134, 191
HpyAV CCTTC 3 cut(s) 64, 166, 271
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 2 cut(s) 189, 261
HpyCH4V TGCA 5 cut(s) 124, 217, 461, 620, 689
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 379, 467
HpyF3I CTNAG 4 cut(s) 63, 96, 252, 368
HpySE526I ACGT 2 cut(s) 189, 261
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 3 cut(s) 277, 326, 548
KspAI GTTAAC 1 cut(s) 268
Kzo9I GATC 6 cut(s) 27, 81, 150, 316, 346, 657
LmnI GCTCC 1 cut(s) 529
Lsp1109I GCAGC 4 cut(s) 136, 229, 296, 673
LweI GCATC 3 cut(s) 204, 296, 607
MaeII ACGT 2 cut(s) 189, 261
MalI GATC 6 cut(s) 29, 83, 152, 318, 348, 659
MboI GATC 6 cut(s) 27, 81, 150, 316, 346, 657
MflI RGATCY 4 cut(s) 27, 81, 346, 657
MluCI AATT 1 cut(s) 104
MmeI TCCRAC 2 cut(s) 315, 383
MnlI CCTC 8 cut(s) 36, 58, 91, 127, 274, 421, 445, 557
MseI TTAA 3 cut(s) 237, 267, 342
MspA1I CMGCKG 1 cut(s) 446
MspR9I CCNGG 1 cut(s) 575
MvaI CCWGG 1 cut(s) 575
MwoI GCNNNNNNNGC 3 cut(s) 31, 379, 467
NdeII GATC 6 cut(s) 27, 81, 150, 316, 346, 657
NlaIII CATG 3 cut(s) 277, 326, 548
PaeR7I CTCGAG 1 cut(s) 582
PaqCI CACCTGC 1 cut(s) 529
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 1 cut(s) 440
PflMI CCANNNNNTGG 1 cut(s) 37
PkrI GCNGC 4 cut(s) 126, 219, 286, 688
Psp6I CCWGG 1 cut(s) 573
PspGI CCWGG 1 cut(s) 573
PsuI RGATCY 4 cut(s) 27, 81, 346, 657
RsaI GTAC 2 cut(s) 329, 435
RsaNI GTAC 2 cut(s) 328, 434
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 3 cut(s) 237, 267, 342
SatI GCNGC 4 cut(s) 125, 218, 285, 687
Sau3AI GATC 6 cut(s) 27, 81, 150, 316, 346, 657
ScrFI CCNGG 1 cut(s) 575
SfaNI GCATC 3 cut(s) 204, 296, 607
Sfr274I CTCGAG 1 cut(s) 582
SlaI CTCGAG 1 cut(s) 582
SmlI CTYRAG 1 cut(s) 582
SmoI CTYRAG 1 cut(s) 582
Sse9I AATT 1 cut(s) 104
SsiI CCGC 5 cut(s) 52, 137, 386, 446, 661
StyD4I CCNGG 1 cut(s) 573
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 2 cut(s) 192, 264
TaqI TCGA 4 cut(s) 132, 186, 212, 583
TasI AATT 1 cut(s) 104
TatI WGTACW 1 cut(s) 433
TfiI GAWTC 1 cut(s) 440
Tru1I TTAA 3 cut(s) 237, 267, 342
Tru9I TTAA 3 cut(s) 237, 267, 342
TseI GCWGC 4 cut(s) 124, 217, 284, 686
TspDTI ATGAA 4 cut(s) 17, 37, 290, 467
TspGWI ACGGA 2 cut(s) 76, 629
Van91I CCANNNNNTGG 1 cut(s) 37
XapI RAATTY 1 cut(s) 104
XhoI CTCGAG 1 cut(s) 582
XmiI GTMKAC 1 cut(s) 186
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.