Rh2BG395300

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007700
Physical Location & Seq
Reverse (-)
56012758 .. 56015202
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG395300.1

Sequence Viewer

Length: 447 bp
ATGGAGTCGAACCTTCCCACAAGGTTTACCCAGAGTTTCAGTCTCTTCGTGTTGTCCACAGAACTGGGTTTCATTTTCGATCTCCCCGCCACTGGATTAACGGAAGGACTTCCTACGTTTGCAGAAATTCAGTGGGACCGAAGTATGTCTGTTGATTTTCGAAACCACGACTTTGGTTTCTGTTTTGGTAGTAGTGCACTAGTGCTCGAGGCCCTCTATGTTGGTTTGCTCGGGCTGAGAGAGTCTGCTGTTTTGTTGCATTGTCGCAAAGATGACCTGCATCTGGTGGAGTCAGTTCTGGAAGCAGCAGCACAGGAATATGCTGGAAAAAGAAATGTTCATGCACCAGAGATCATAGTTGACCACAATATCTATCTTCCACTTGCTCCCACCCATCCTAATCCCTATGATAATTCTGATTCTGGAGGTGTAGTCTTGGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
vATP-synt_E

Protein Analysis

148

Amino Acids

16.28

Weight (kDa)

4.7

Isoelectric Point (pI)

50.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
vATP-synt_E PF01991 73 - 147 6.9e-09 ATP synthase (E/31 kDa) subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 285
AciI CCGC 1 cut(s) 87
AcsI RAATTY 1 cut(s) 126
AfiI CCNNNNNNNGG 2 cut(s) 92, 283
AhlI ACTAGT 1 cut(s) 199
Alw21I GWGCWC 2 cut(s) 199, 207
Alw26I GTCTC 1 cut(s) 47
Alw44I GTGCAC 1 cut(s) 195
Ama87I CYCGRG 2 cut(s) 206, 230
AoxI GGCC 1 cut(s) 210
ApaLI GTGCAC 1 cut(s) 195
ApeKI GCWGC 2 cut(s) 305, 308
ApoI RAATTY 1 cut(s) 126
Asp700I GAANNNNTTC 1 cut(s) 108
AspS9I GGNCC 2 cut(s) 136, 211
AsuII TTCGAA 1 cut(s) 160
AvaI CYCGRG 2 cut(s) 206, 230
AvaII GGWCC 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 199
Bbv12I GWGCWC 2 cut(s) 199, 207
BbvI GCAGC 2 cut(s) 317, 320
BccI CCATC 1 cut(s) 402
BcoDI GTCTC 1 cut(s) 47
BcuI ACTAGT 1 cut(s) 199
BfaI CTAG 1 cut(s) 200
BfuAI ACCTGC 1 cut(s) 285
BisI GCNGC 2 cut(s) 306, 309
BlsI GCNGC 2 cut(s) 307, 310
Bme18I GGWCC 1 cut(s) 136
BmeT110I CYCGRG 2 cut(s) 206, 230
BmgT120I GGNCC 2 cut(s) 136, 211
BmiI GGNNCC 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 74
BmsI GCATC 1 cut(s) 289
BmuI ACTGGG 1 cut(s) 74
BpmI CTGGAG 1 cut(s) 444
Bpu14I TTCGAA 1 cut(s) 160
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 283
Bse1I ACTGG 2 cut(s) 69, 97
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 2 cut(s) 92, 283
BseMII CTCAG 1 cut(s) 227
BseNI ACTGG 2 cut(s) 69, 97
BseSI GKGCMC 1 cut(s) 199
BseXI GCAGC 2 cut(s) 317, 320
BshFI GGCC 1 cut(s) 212
BsiHKAI GWGCWC 2 cut(s) 199, 207
BsiHKCI CYCGRG 2 cut(s) 206, 230
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 2 cut(s) 92, 283
BsmAI GTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 1 cut(s) 212
BsoBI CYCGRG 2 cut(s) 206, 230
Bsp119I TTCGAA 1 cut(s) 160
Bsp1286I GDGCHC 2 cut(s) 199, 207
Bsp143I GATC 2 cut(s) 79, 351
BspACI CCGC 1 cut(s) 87
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 1 cut(s) 228
BspLI GGNNCC 1 cut(s) 137
BspMI ACCTGC 1 cut(s) 285
BspT104I TTCGAA 1 cut(s) 160
BsrI ACTGG 2 cut(s) 69, 97
BssMI GATC 2 cut(s) 79, 351
Bst6I CTCTTC 1 cut(s) 50
BstBI TTCGAA 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 236
BstF5I GGATG 1 cut(s) 394
BstKTI GATC 2 cut(s) 82, 354
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 2 cut(s) 79, 351
BstSLI GKGCMC 1 cut(s) 199
BstV1I GCAGC 2 cut(s) 317, 320
BstXI CCANNNNNNTGG 2 cut(s) 64, 173
BsuRI GGCC 1 cut(s) 212
BtsCI GGATG 1 cut(s) 394
BtsIMutI CAGTG 2 cut(s) 90, 137
BveI ACCTGC 1 cut(s) 285
Cfr13I GGNCC 2 cut(s) 136, 211
CviAII CATG 1 cut(s) 341
CviJI RGCY 3 cut(s) 212, 235, 440
CviKI_1 RGCY 3 cut(s) 212, 235, 440
DdeI CTNAG 1 cut(s) 236
DpnI GATC 2 cut(s) 81, 353
DpnII GATC 2 cut(s) 79, 351
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
Eco47I GGWCC 1 cut(s) 136
Eco88I CYCGRG 2 cut(s) 206, 230
EcoO109I RGGNCCY 1 cut(s) 211
FaeI CATG 1 cut(s) 344
FaiI YATR 6 cut(s) 146, 219, 321, 342, 356, 408
FaqI GGGAC 1 cut(s) 149
FatI CATG 1 cut(s) 340
FauI CCCGC 1 cut(s) 94
Fnu4HI GCNGC 2 cut(s) 306, 309
FokI GGATG 1 cut(s) 381
Fsp4HI GCNGC 2 cut(s) 306, 309
FspBI CTAG 1 cut(s) 200
GluI GCNGC 2 cut(s) 306, 309
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 1 cut(s) 212
Hin1II CATG 1 cut(s) 344
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 4 cut(s) 5, 242, 290, 419
Hpy166II GTNNAC 4 cut(s) 27, 57, 197, 361
Hpy188I TCNGA 1 cut(s) 418
Hpy188III TCNNGA 3 cut(s) 299, 423, 444
Hpy8I GTNNAC 4 cut(s) 27, 57, 197, 361
HpyAV CCTTC 2 cut(s) 23, 98
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 5 cut(s) 122, 197, 259, 280, 344
HpyF3I CTNAG 1 cut(s) 236
HpySE526I ACGT 1 cut(s) 116
Hsp92II CATG 1 cut(s) 344
Kzo9I GATC 2 cut(s) 79, 351
LmnI GCTCC 1 cut(s) 391
Lsp1109I GCAGC 2 cut(s) 317, 320
LweI GCATC 1 cut(s) 289
MaeI CTAG 1 cut(s) 200
MaeII ACGT 1 cut(s) 116
MalI GATC 2 cut(s) 81, 353
MboI GATC 2 cut(s) 79, 351
MboII GAAGA 2 cut(s) 37, 368
MhlI GDGCHC 2 cut(s) 199, 207
MluCI AATT 2 cut(s) 126, 412
MlyI GAGTC 3 cut(s) 14, 251, 299
MnlI CCTC 3 cut(s) 202, 224, 419
MroXI GAANNNNTTC 1 cut(s) 108
MseI TTAA 1 cut(s) 98
NdeII GATC 2 cut(s) 79, 351
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 1 cut(s) 137
NspV TTCGAA 1 cut(s) 160
PaeR7I CTCGAG 1 cut(s) 206
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 2 cut(s) 307, 310
PleI GAGTC 3 cut(s) 13, 250, 298
PpsI GAGTC 3 cut(s) 13, 250, 298
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 2 cut(s) 136, 211
PspXI VCTCGAGB 1 cut(s) 206
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 2 cut(s) 306, 309
Sau3AI GATC 2 cut(s) 79, 351
Sau96I GGNCC 2 cut(s) 136, 211
SchI GAGTC 3 cut(s) 14, 251, 299
SduI GDGCHC 2 cut(s) 199, 207
SetI ASST 5 cut(s) 15, 26, 119, 279, 430
SfaNI GCATC 1 cut(s) 289
Sfr274I CTCGAG 1 cut(s) 206
SfuI TTCGAA 1 cut(s) 160
SinI GGWCC 1 cut(s) 136
SlaI CTCGAG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
SpeI ACTAGT 1 cut(s) 199
Sse9I AATT 2 cut(s) 126, 412
SsiI CCGC 1 cut(s) 87
SspMI CTAG 1 cut(s) 200
TaiI ACGT 1 cut(s) 119
TaqI TCGA 4 cut(s) 8, 78, 160, 207
TaqII GACCGA 1 cut(s) 153
TasI AATT 2 cut(s) 126, 412
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TscAI CASTG 2 cut(s) 97, 137
TseI GCWGC 2 cut(s) 305, 308
TspDTI ATGAA 2 cut(s) 61, 329
TspGWI ACGGA 1 cut(s) 116
TspRI CASTG 2 cut(s) 97, 137
VneI GTGCAC 1 cut(s) 195
VpaK11BI GGWCC 1 cut(s) 136
XapI RAATTY 1 cut(s) 126
XhoI CTCGAG 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.