FvH4_5g04480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006624
Physical Location & Seq
Forward (+)
2617264 .. 2620789
3526 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04480.t4

Sequence Viewer

Length: 1119 bp
ATGGAGAAGCGTTTTGCTCTTTTGGTCGGGGTTCTGCTCTTGCAGCTTATGATTCATTGCTCTGGTGCTGATTTGAAGGTAGAAGAAGGTGCAAAAACTGTAGTGGATCCAAAAGTTTCATCGACATCTGAAGGTTCAAATAGCTCGGATGATAAGAAACAGAAAGTTGTGACGAATTTAGTTTCAGACGGTAATGAAGTTCAAGAAGTGAAGAAAGATAAAGATCAGGGTGGTGGATCAAATAATGGTGTTGGGAAAAGCAAGGAGAAGACCGGTTCAGATGGGGAAGTTGGGTCGACAGAAACGCATAGTGTGGCAAAGGGTGAGAAAGGATCAAATGATGGGAAGAATGGGAAGTCGAGTGAGGAATCAAAGGCAATGGCTAGGGAGGAGGTGGGTAATGCCGGAAATGTGAACCCTGTGAGAGAGGATGGTACTCCCCGTGAGGAATGTGGTTCTGCTAATATGTGCACTGTAAAGGAGAACAAGCTGGTTGCCTGTTTGAGAGTTCCTGGAGATGATGATTCTCCTCATCTTTCGCTTTTAATTCAGAACAAGGGTAAAGACCCTCTGGTGGTTACCATTTCAGCTCCTGAATTTGTCCGGCTAGACAAAACAAAAGTTCAACTGAAAGAGAAAGATAATGCAAAGGTGGATGTTTCTGTTGGAAGTGGAGGAGCTACCAGCATAATTGTTCTGAAGGCAGGAAATGGCAACTGCAGCCTTGATTTCAAGGACCTGATCACGCACAGTTCCCAGAAGGAGCCTGATAATTCTTCAAACACCACCTACTTGTTCCTCTGGACACACAGACCTGCCATTGGGATTCTATTGGTTGCCTTGCTAATGATTTTGGTATTTGCCGGGATGTATGTCAGATTCATGAAGAAGCGGGTTTCCAGCAGTGGCTTTAAATATCAGAAGCTTGATGATGTGCACCTCCCAGTTTTGAGCAGTGAAAAGCCAGAGTTGCACATTAATGATGGATGGGACGACACTTGGGATGATAAGTGGGATGATGAGGAGGCCCCCCATACTCCCTCGATGCCGGTTACTCCTAGCCTCTCTGGTAAAGGCCTTGCCTCACGAAGGTTGAACAAGGAAGGGTGGAAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

373

Amino Acids

40.16

Weight (kDa)

5.85

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7356 PF24053 144 - 247 9.7e-31 Domain of unknown function (DUF7356)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 823
AccI GTMKAC 1 cut(s) 296
AciI CCGC 1 cut(s) 892
AclWI GGATC 4 cut(s) 101, 114, 244, 340
AcsI RAATTY 2 cut(s) 175, 596
AcuI CTGAAG 2 cut(s) 150, 719
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 3 cut(s) 574, 821, 1089
AgeI ACCGGT 1 cut(s) 272
AgsI TTSAA 7 cut(s) 76, 138, 203, 626, 733, 780, 1096
AjnI CCWGG 1 cut(s) 511
AluBI AGCT 6 cut(s) 46, 144, 490, 590, 680, 925
AluI AGCT 6 cut(s) 46, 144, 490, 590, 680, 925
Alw21I GWGCWC 2 cut(s) 473, 939
Alw44I GTGCAC 2 cut(s) 469, 935
AlwI GGATC 4 cut(s) 101, 114, 244, 340
AlwNI CAGNNNCTG 1 cut(s) 593
AoxI GGCC 2 cut(s) 1026, 1075
ApaLI GTGCAC 2 cut(s) 469, 935
ApeKI GCWGC 2 cut(s) 43, 720
ApoI RAATTY 2 cut(s) 175, 596
AseI ATTAAT 1 cut(s) 978
AsiGI ACCGGT 1 cut(s) 272
AspS9I GGNCC 2 cut(s) 736, 1027
AsuC2I CCSGG 1 cut(s) 865
AsuHPI GGTGA 1 cut(s) 335
AvaII GGWCC 1 cut(s) 736
BaeGI GKGCMC 2 cut(s) 473, 939
BamHI GGATCC 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 275
Bbv12I GWGCWC 2 cut(s) 473, 939
BbvI GCAGC 2 cut(s) 55, 732
BccI CCATC 5 cut(s) 275, 335, 425, 977, 981
BciT130I CCWGG 1 cut(s) 513
BclI TGATCA 1 cut(s) 741
BcnI CCSGG 1 cut(s) 865
BfaI CTAG 3 cut(s) 384, 608, 1059
BfmI CTRYAG 2 cut(s) 99, 718
BfuAI ACCTGC 1 cut(s) 823
BisI GCNGC 2 cut(s) 44, 721
BlsI GCNGC 2 cut(s) 45, 722
Bme1390I CCNGG 2 cut(s) 513, 865
Bme18I GGWCC 1 cut(s) 736
BmgT120I GGNCC 2 cut(s) 736, 1027
BmiI GGNNCC 3 cut(s) 108, 765, 1029
BmrFI CCNGG 2 cut(s) 513, 865
BmrI ACTGGG 1 cut(s) 938
BmsI GCATC 1 cut(s) 1035
BmuI ACTGGG 1 cut(s) 938
BpiI GAAGAC 1 cut(s) 275
BpmI CTGGAG 1 cut(s) 534
BpuMI CCSGG 1 cut(s) 865
BsaBI GATNNNNATC 1 cut(s) 222
BsaWI WCCGGW 1 cut(s) 272
Bsc4I CCNNNNNNNGG 3 cut(s) 574, 821, 1089
Bse118I RCCGGY 2 cut(s) 272, 1048
Bse1I ACTGG 1 cut(s) 944
Bse3DI GCAATG 2 cut(s) 55, 384
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 1 cut(s) 513
BseGI GGATG 7 cut(s) 154, 436, 661, 873, 992, 1009, 1021
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 3 cut(s) 574, 821, 1089
BseMI GCAATG 2 cut(s) 55, 384
BseNI ACTGG 1 cut(s) 944
BseRI GAGGAG 4 cut(s) 404, 519, 690, 1037
BseSI GKGCMC 2 cut(s) 473, 939
BseXI GCAGC 2 cut(s) 55, 732
BshFI GGCC 2 cut(s) 1028, 1077
BshTI ACCGGT 1 cut(s) 272
BsiHKAI GWGCWC 2 cut(s) 473, 939
BsiSI CCGG 5 cut(s) 273, 405, 604, 864, 1049
BslFI GGGAC 1 cut(s) 1004
BslI CCNNNNNNNGG 3 cut(s) 574, 821, 1089
BsmFI GGGAC 1 cut(s) 1004
BsnI GGCC 2 cut(s) 1028, 1077
Bsp1286I GDGCHC 2 cut(s) 473, 939
Bsp143I GATC 5 cut(s) 106, 223, 236, 332, 741
BspACI CCGC 1 cut(s) 892
BspANI GGCC 2 cut(s) 1028, 1077
BspHI TCATGA 1 cut(s) 882
BspLI GGNNCC 3 cut(s) 108, 765, 1029
BspMAI CTGCAG 1 cut(s) 722
BspMI ACCTGC 1 cut(s) 823
BspPI GGATC 4 cut(s) 101, 114, 244, 340
BsrDI GCAATG 2 cut(s) 55, 384
BsrFI RCCGGY 2 cut(s) 272, 1048
BsrI ACTGG 1 cut(s) 944
BssAI RCCGGY 2 cut(s) 272, 1048
BssMI GATC 5 cut(s) 106, 223, 236, 332, 741
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 4 cut(s) 100, 191, 475, 752
BstEII GGTNACC 1 cut(s) 577
BstENI CCTNNNNNAGG 1 cut(s) 1087
BstF5I GGATG 7 cut(s) 154, 436, 661, 873, 992, 1009, 1021
BstKTI GATC 5 cut(s) 109, 226, 239, 335, 744
BstMBI GATC 5 cut(s) 106, 223, 236, 332, 741
BstMWI GCNNNNNNNGC 3 cut(s) 43, 720, 970
BstNI CCWGG 1 cut(s) 513
BstPI GGTNACC 1 cut(s) 577
BstSCI CCNGG 2 cut(s) 511, 863
BstSFI CTRYAG 2 cut(s) 99, 718
BstSLI GKGCMC 2 cut(s) 473, 939
BstV1I GCAGC 2 cut(s) 55, 732
BstV2I GAAGAC 1 cut(s) 275
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BsuRI GGCC 2 cut(s) 1028, 1077
BtsCI GGATG 7 cut(s) 154, 436, 661, 873, 992, 1009, 1021
BtsI GCAGTG 2 cut(s) 910, 961
BtsIMutI CAGTG 3 cut(s) 471, 910, 961
BveI ACCTGC 1 cut(s) 823
CaiI CAGNNNCTG 1 cut(s) 593
CciI TCATGA 1 cut(s) 882
Cfr10I RCCGGY 2 cut(s) 272, 1048
Cfr13I GGNCC 2 cut(s) 736, 1027
Csp6I GTAC 1 cut(s) 435
CspAI ACCGGT 1 cut(s) 272
CviAII CATG 1 cut(s) 883
CviQI GTAC 1 cut(s) 435
DpnI GATC 5 cut(s) 108, 225, 238, 334, 743
DpnII GATC 5 cut(s) 106, 223, 236, 332, 741
DraI TTTAAA 1 cut(s) 913
Eco147I AGGCCT 1 cut(s) 1077
Eco47I GGWCC 1 cut(s) 736
Eco57I CTGAAG 2 cut(s) 150, 719
Eco91I GGTNACC 1 cut(s) 577
EcoNI CCTNNNNNAGG 1 cut(s) 1087
EcoO109I RGGNCCY 2 cut(s) 736, 1027
EcoO65I GGTNACC 1 cut(s) 577
EcoRII CCWGG 1 cut(s) 511
FaeI CATG 1 cut(s) 886
FaiI YATR 7 cut(s) 50, 309, 467, 689, 873, 884, 1035
FaqI GGGAC 1 cut(s) 1004
FatI CATG 1 cut(s) 882
FauI CCCGC 1 cut(s) 885
FbaI TGATCA 1 cut(s) 741
FblI GTMKAC 1 cut(s) 296
Fnu4HI GCNGC 2 cut(s) 44, 721
FokI GGATG 7 cut(s) 161, 443, 668, 880, 999, 1016, 1028
Fsp4HI GCNGC 2 cut(s) 44, 721
FspBI CTAG 3 cut(s) 384, 608, 1059
GluI GCNGC 2 cut(s) 44, 721
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 2 cut(s) 1028, 1077
HapII CCGG 5 cut(s) 273, 405, 604, 864, 1049
Hin1II CATG 1 cut(s) 886
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 5 cut(s) 52, 368, 524, 826, 879
HpaII CCGG 5 cut(s) 273, 405, 604, 864, 1049
HphI GGTGA 1 cut(s) 335
Hpy166II GTNNAC 4 cut(s) 297, 415, 471, 937
Hpy188I TCNGA 8 cut(s) 130, 148, 187, 280, 552, 699, 878, 921
Hpy188III TCNNGA 5 cut(s) 203, 593, 802, 883, 1086
Hpy8I GTNNAC 4 cut(s) 297, 415, 471, 937
HpyAV CCTTC 7 cut(s) 70, 80, 125, 694, 754, 1083, 1097
HpyCH4III ACNGT 4 cut(s) 100, 191, 475, 752
HpyCH4V TGCA 7 cut(s) 43, 92, 471, 647, 720, 937, 973
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 720, 970
Hsp92II CATG 1 cut(s) 886
Ksp22I TGATCA 1 cut(s) 741
Kzo9I GATC 5 cut(s) 106, 223, 236, 332, 741
LmnI GCTCC 3 cut(s) 595, 677, 763
Lsp1109I GCAGC 2 cut(s) 55, 732
LweI GCATC 1 cut(s) 1035
MaeI CTAG 3 cut(s) 384, 608, 1059
MaeIII GTNAC 3 cut(s) 169, 577, 1051
MalI GATC 5 cut(s) 108, 225, 238, 334, 743
MboI GATC 5 cut(s) 106, 223, 236, 332, 741
MboII GAAGA 6 cut(s) 95, 223, 280, 358, 768, 898
MflI RGATCY 1 cut(s) 106
MhlI GDGCHC 2 cut(s) 473, 939
MluCI AATT 5 cut(s) 175, 546, 596, 690, 772
MmeI TCCRAC 1 cut(s) 646
MseI TTAA 3 cut(s) 545, 912, 978
MslI CAYNNNNRTG 1 cut(s) 978
MspI CCGG 5 cut(s) 273, 405, 604, 864, 1049
MspR9I CCNGG 2 cut(s) 513, 865
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 3 cut(s) 43, 720, 970
NciI CCSGG 1 cut(s) 865
NdeII GATC 5 cut(s) 106, 223, 236, 332, 741
NlaIII CATG 1 cut(s) 886
NlaIV GGNNCC 3 cut(s) 108, 765, 1029
NmuCI GTSAC 1 cut(s) 169
PagI TCATGA 1 cut(s) 882
PceI AGGCCT 1 cut(s) 1077
PfeI GAWTC 5 cut(s) 52, 368, 524, 826, 879
PfoI TCCNGGA 1 cut(s) 511
PinAI ACCGGT 1 cut(s) 272
PkrI GCNGC 2 cut(s) 45, 722
PpuMI RGGWCCY 1 cut(s) 736
PshBI ATTAAT 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 736
Psp6I CCWGG 1 cut(s) 511
PspEI GGTNACC 1 cut(s) 577
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 3 cut(s) 108, 765, 1029
PspPI GGNCC 2 cut(s) 736, 1027
PspPPI RGGWCCY 1 cut(s) 736
PstI CTGCAG 1 cut(s) 722
PstNI CAGNNNCTG 1 cut(s) 593
PsuI RGATCY 1 cut(s) 106
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 978
SalI GTCGAC 1 cut(s) 295
SaqAI TTAA 3 cut(s) 545, 912, 978
SatI GCNGC 2 cut(s) 44, 721
Sau3AI GATC 5 cut(s) 106, 223, 236, 332, 741
Sau96I GGNCC 2 cut(s) 736, 1027
ScrFI CCNGG 2 cut(s) 513, 865
SduI GDGCHC 2 cut(s) 473, 939
SfaNI GCATC 1 cut(s) 1035
SfcI CTRYAG 2 cut(s) 99, 718
SinI GGWCC 1 cut(s) 736
SmiMI CAYNNNNRTG 1 cut(s) 978
Sse9I AATT 5 cut(s) 175, 546, 596, 690, 772
SseBI AGGCCT 1 cut(s) 1077
SsiI CCGC 1 cut(s) 892
SspMI CTAG 3 cut(s) 384, 608, 1059
StuI AGGCCT 1 cut(s) 1077
StyD4I CCNGG 2 cut(s) 511, 863
TaaI ACNGT 4 cut(s) 100, 191, 475, 752
TaqI TCGA 4 cut(s) 122, 296, 359, 1043
TasI AATT 5 cut(s) 175, 546, 596, 690, 772
TfiI GAWTC 5 cut(s) 52, 368, 524, 826, 879
Tru1I TTAA 3 cut(s) 545, 912, 978
Tru9I TTAA 3 cut(s) 545, 912, 978
TscAI CASTG 3 cut(s) 478, 910, 961
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 2 cut(s) 43, 720
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 5 cut(s) 44, 108, 210, 871, 899
TspRI CASTG 3 cut(s) 478, 910, 961
VneI GTGCAC 2 cut(s) 469, 935
VpaK11BI GGWCC 1 cut(s) 736
VspI ATTAAT 1 cut(s) 978
XagI CCTNNNNNAGG 1 cut(s) 1087
XapI RAATTY 2 cut(s) 175, 596
XmiI GTMKAC 1 cut(s) 296
XspI CTAG 3 cut(s) 384, 608, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.