MD06G1105600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006624
Physical Location & Seq
Forward (+)
24417017 .. 24420819
3803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1105600.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGGAGAAGAATTTTGTTCTGTTGGTTGGGATTTTATTGTTGCAGATTGTAGTTCATTGCTCTGGTGCTGATTCGAAGGTAGAATCCGGTGTGAAAACTGATTTGGGTCGGAAAAGTTCGTTAGCATCGACTAATTCAAATGGTTCCAATGATAACACTGTTGTGTCTAATTTGGTTTTGAATGCTAACGATGTTCAAAAATTGAAGAAAGACGGAGACCAGGTGGTTGGATCAAAGGAGGGTGATGCGGATAAGAAGAGCAATCCAAGTAAGGAAGTGGGTGTGAAAGAATCTGATACTGTGGCAAAGAGTGGTGGAGGATTGAGTGAGGGATCCAAAATAGAGGGCGAGGACAAGAAACAGAAGCCGAGTGATGGGTTGGAATCAAAGGAAGTGCCTAAGGAGGTGGATAATGGAGGAAATGCAATCTCTGTGAACCCCATGAGGAAGGAGGGGACTCGGAGTGAGGAATGTGGTCTATCTAATATGTGCACTGATAAGGGCAACCAGCTGGTTGCGTGTTTGAGAGTTCCCGGAAATGATTCTCCCCATCTTTCACTTTTAATTAAGAACAAGGCCAAAGGCCCTCTCTTGGTTACCATTGTAGCTCCTGGTTTTGTGAGACTGGACAAAACAAAAATTCAACTTGAAGAGAAAGAAAACAGAAAGGTTTTGGTTTCAATTGGAAAGGGAGGAGCTGGCGGTTCAATTGTGCTAAAAGCAGGAAATGGTCAATGCAACCTTGATTTGAAGGACCTGGTCACACACAGTTCCAGGAAGGAGCCTGAGAATTCTTCAAACTCTGCCTACACAAACTTCCTAAAACAAAAACCTACGATTGCAGTTGTGTTCTTTGCTTCAGTAATGATATTGGCATCAGCTTGGATGTGCATCAGCTTTAGGAATAGGCGGTTCTCTGGCAATGGGTTGAAATATCAGAAGCTAGATGAGGACCTGCCAATTTCCAATGAGGAAAAACCGGTGTTGCAGGTTAACGATGGATGGGATAATAACTGGGACGATAATTGGGATGATGAGGAGGCACCCCACACACCCTCAATGCCGATTACTCCTAGTCTCTCTGGCAAAGGCCTCGCCTCACGACGATTGAACAAGGAAGCGTGGAAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

378

Amino Acids

40.63

Weight (kDa)

7.55

Isoelectric Point (pI)

30.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7356 PF24053 150 - 253 9.9e-31 Domain of unknown function (DUF7356)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 963, 979
AccB1I GGYRCC 1 cut(s) 1042
AciI CCGC 3 cut(s) 248, 702, 910
AclWI GGATC 3 cut(s) 238, 327, 340
AcsI RAATTY 3 cut(s) 10, 639, 790
AcuI CTGAAG 1 cut(s) 843
AfiI CCNNNNNNNGG 2 cut(s) 374, 592
AgeI ACCGGT 1 cut(s) 979
AjnI CCWGG 4 cut(s) 219, 610, 756, 773
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AleI CACNNNNGTG 1 cut(s) 161
AluBI AGCT 6 cut(s) 511, 608, 698, 881, 897, 943
AluI AGCT 6 cut(s) 511, 608, 698, 881, 897, 943
Alw21I GWGCWC 1 cut(s) 494
Alw26I GTCTC 3 cut(s) 210, 616, 1082
Alw44I GTGCAC 1 cut(s) 490
AlwI GGATC 3 cut(s) 238, 327, 340
AoxI GGCC 3 cut(s) 576, 583, 1090
ApaLI GTGCAC 1 cut(s) 490
ApoI RAATTY 3 cut(s) 10, 639, 790
AsiGI ACCGGT 1 cut(s) 979
Asp700I GAANNNNTTC 1 cut(s) 541
AspS9I GGNCC 3 cut(s) 584, 754, 952
AsuC2I CCSGG 1 cut(s) 534
AsuHPI GGTGA 1 cut(s) 254
AsuII TTCGAA 1 cut(s) 74
AvaII GGWCC 2 cut(s) 754, 952
AxyI CCTNAGG 1 cut(s) 399
BaeGI GKGCMC 1 cut(s) 494
BamHI GGATCC 1 cut(s) 332
BanI GGYRCC 1 cut(s) 1042
Bbv12I GWGCWC 1 cut(s) 494
BccI CCATC 4 cut(s) 368, 558, 992, 996
BcgI CGANNNNNNTGC 2 cut(s) 1075, 1109
BciT130I CCWGG 4 cut(s) 221, 612, 758, 775
BcnI CCSGG 1 cut(s) 534
BcoDI GTCTC 3 cut(s) 210, 616, 1082
BfaI CTAG 2 cut(s) 944, 1074
BfuAI ACCTGC 2 cut(s) 963, 979
Bme1390I CCNGG 5 cut(s) 221, 534, 612, 758, 775
Bme18I GGWCC 2 cut(s) 754, 952
BmgT120I GGNCC 3 cut(s) 584, 754, 952
BmiI GGNNCC 4 cut(s) 145, 334, 783, 1044
BmrFI CCNGG 5 cut(s) 221, 534, 612, 758, 775
BmrI ACTGGG 1 cut(s) 1024
BmsI GCATC 4 cut(s) 134, 235, 884, 900
BmuI ACTGGG 1 cut(s) 1024
Bpu14I TTCGAA 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 534
BsaBI GATNNNNATC 1 cut(s) 890
BsaI GGTCTC 1 cut(s) 210
BsaWI WCCGGW 2 cut(s) 86, 979
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 2 cut(s) 374, 592
Bse118I RCCGGY 1 cut(s) 979
Bse1I ACTGG 2 cut(s) 630, 1019
Bse21I CCTNAGG 1 cut(s) 399
Bse3DI GCAATG 2 cut(s) 55, 928
Bse8I GATNNNNATC 1 cut(s) 890
BseBI CCWGG 4 cut(s) 221, 612, 758, 775
BseGI GGATG 3 cut(s) 891, 1007, 1036
BseJI GATNNNNATC 1 cut(s) 890
BseLI CCNNNNNNNGG 2 cut(s) 374, 592
BseMI GCAATG 2 cut(s) 55, 928
BseMII CTCAG 1 cut(s) 777
BseNI ACTGG 2 cut(s) 630, 1019
BseRI GAGGAG 2 cut(s) 708, 1052
BseSI GKGCMC 1 cut(s) 494
BshFI GGCC 3 cut(s) 578, 585, 1092
BshNI GGYRCC 1 cut(s) 1042
BshTI ACCGGT 1 cut(s) 979
BsiHKAI GWGCWC 1 cut(s) 494
BsiSI CCGG 3 cut(s) 87, 534, 980
BslFI GGGAC 2 cut(s) 469, 1031
BslI CCNNNNNNNGG 2 cut(s) 374, 592
BsmAI GTCTC 3 cut(s) 210, 616, 1082
BsmFI GGGAC 2 cut(s) 469, 1031
BsmI GAATGC 1 cut(s) 187
BsnI GGCC 3 cut(s) 578, 585, 1092
Bso31I GGTCTC 1 cut(s) 210
Bsp119I TTCGAA 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 494
Bsp143I GATC 2 cut(s) 230, 332
BspACI CCGC 3 cut(s) 248, 702, 910
BspANI GGCC 3 cut(s) 578, 585, 1092
BspCNI CTCAG 1 cut(s) 778
BspLI GGNNCC 4 cut(s) 145, 334, 783, 1044
BspMI ACCTGC 2 cut(s) 963, 979
BspPI GGATC 3 cut(s) 238, 327, 340
BspQI GCTCTTC 1 cut(s) 251
BspT104I TTCGAA 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 1042
BspTNI GGTCTC 1 cut(s) 210
BsrDI GCAATG 2 cut(s) 55, 928
BsrFI RCCGGY 1 cut(s) 979
BsrI ACTGG 2 cut(s) 630, 1019
BssAI RCCGGY 1 cut(s) 979
BssMI GATC 2 cut(s) 230, 332
Bst2UI CCWGG 4 cut(s) 221, 612, 758, 775
Bst4CI ACNGT 3 cut(s) 160, 301, 770
Bst6I CTCTTC 2 cut(s) 251, 645
BstBI TTCGAA 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 700
BstDEI CTNAG 2 cut(s) 399, 786
BstEII GGTNACC 1 cut(s) 595
BstF5I GGATG 3 cut(s) 891, 1007, 1036
BstKTI GATC 2 cut(s) 233, 335
BstMAI GTCTC 3 cut(s) 210, 616, 1082
BstMBI GATC 2 cut(s) 230, 332
BstNI CCWGG 4 cut(s) 221, 612, 758, 775
BstPI GGTNACC 1 cut(s) 595
BstSCI CCNGG 5 cut(s) 219, 532, 610, 756, 773
BstSLI GKGCMC 1 cut(s) 494
BstX2I RGATCY 1 cut(s) 332
BstXI CCANNNNNNTGG 1 cut(s) 227
BstYI RGATCY 1 cut(s) 332
Bsu36I CCTNAGG 1 cut(s) 399
BsuRI GGCC 3 cut(s) 578, 585, 1092
BtsCI GGATG 3 cut(s) 891, 1007, 1036
BtsIMutI CAGTG 2 cut(s) 156, 492
BveI ACCTGC 2 cut(s) 963, 979
Cac8I GCNNGC 1 cut(s) 700
Cfr10I RCCGGY 1 cut(s) 979
Cfr13I GGNCC 3 cut(s) 584, 754, 952
CsiI ACCWGGT 2 cut(s) 219, 756
CspAI ACCGGT 1 cut(s) 979
CviAII CATG 1 cut(s) 442
DdeI CTNAG 2 cut(s) 399, 786
DpnI GATC 2 cut(s) 232, 334
DpnII GATC 2 cut(s) 230, 332
Eam1104I CTCTTC 2 cut(s) 251, 645
EarI CTCTTC 2 cut(s) 251, 645
Eco147I AGGCCT 1 cut(s) 1092
Eco31I GGTCTC 1 cut(s) 210
Eco47I GGWCC 2 cut(s) 754, 952
Eco57I CTGAAG 1 cut(s) 843
Eco81I CCTNAGG 1 cut(s) 399
Eco91I GGTNACC 1 cut(s) 595
EcoO109I RGGNCCY 3 cut(s) 584, 754, 952
EcoO65I GGTNACC 1 cut(s) 595
EcoRI GAATTC 1 cut(s) 790
EcoRII CCWGG 4 cut(s) 219, 610, 756, 773
FaeI CATG 1 cut(s) 445
FaiI YATR 2 cut(s) 443, 488
FaqI GGGAC 2 cut(s) 469, 1031
FatI CATG 1 cut(s) 441
FokI GGATG 3 cut(s) 898, 1014, 1043
FspBI CTAG 2 cut(s) 944, 1074
HaeIII GGCC 3 cut(s) 578, 585, 1092
HapII CCGG 3 cut(s) 87, 534, 980
Hin1II CATG 1 cut(s) 445
HincII GTYRAC 1 cut(s) 994
HindII GTYRAC 1 cut(s) 994
HinfI GANTC 6 cut(s) 71, 83, 290, 383, 457, 542
HpaI GTTAAC 1 cut(s) 994
HpaII CCGG 3 cut(s) 87, 534, 980
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 3 cut(s) 436, 492, 994
Hpy188I TCNGA 4 cut(s) 111, 295, 462, 939
Hpy188III TCNNGA 1 cut(s) 1101
Hpy8I GTNNAC 3 cut(s) 436, 492, 994
Hpy99I CGWCG 1 cut(s) 1107
HpyAV CCTTC 4 cut(s) 70, 442, 745, 772
HpyCH4III ACNGT 3 cut(s) 160, 301, 770
HpyCH4V TGCA 7 cut(s) 43, 425, 492, 738, 842, 891, 988
HpyF3I CTNAG 2 cut(s) 399, 786
Hsp92II CATG 1 cut(s) 445
KspAI GTTAAC 1 cut(s) 994
Kzo9I GATC 2 cut(s) 230, 332
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 3 cut(s) 613, 695, 781
LweI GCATC 4 cut(s) 134, 235, 884, 900
MabI ACCWGGT 2 cut(s) 219, 756
MaeI CTAG 2 cut(s) 944, 1074
MaeIII GTNAC 2 cut(s) 595, 760
MalI GATC 2 cut(s) 232, 334
MboI GATC 2 cut(s) 230, 332
MboII GAAGA 5 cut(s) 19, 217, 268, 662, 786
MfeI CAATTG 2 cut(s) 681, 708
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 1 cut(s) 494
MlyI GAGTC 1 cut(s) 451
MmeI TCCRAC 3 cut(s) 89, 208, 360
MroXI GAANNNNTTC 1 cut(s) 541
MseI TTAA 3 cut(s) 563, 567, 993
MslI CAYNNNNRTG 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 511
MspI CCGG 3 cut(s) 87, 534, 980
MspR9I CCNGG 5 cut(s) 221, 534, 612, 758, 775
MunI CAATTG 2 cut(s) 681, 708
Mva1269I GAATGC 1 cut(s) 187
MvaI CCWGG 4 cut(s) 221, 612, 758, 775
NciI CCSGG 1 cut(s) 534
NdeII GATC 2 cut(s) 230, 332
NlaIII CATG 1 cut(s) 445
NlaIV GGNNCC 4 cut(s) 145, 334, 783, 1044
NmeAIII GCCGAG 1 cut(s) 393
NmuCI GTSAC 1 cut(s) 760
NspV TTCGAA 1 cut(s) 74
OliI CACNNNNGTG 1 cut(s) 161
PacI TTAATTAA 1 cut(s) 567
PceI AGGCCT 1 cut(s) 1092
PciSI GCTCTTC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PctI GAATGC 1 cut(s) 187
PdmI GAANNNNTTC 1 cut(s) 541
PfeI GAWTC 5 cut(s) 71, 83, 290, 383, 542
PflFI GACNNNGTC 1 cut(s) 758
PfoI TCCNGGA 2 cut(s) 532, 773
PinAI ACCGGT 1 cut(s) 979
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PpuMI RGGWCCY 2 cut(s) 754, 952
Psp5II RGGWCCY 2 cut(s) 754, 952
Psp6I CCWGG 4 cut(s) 219, 610, 756, 773
PspEI GGTNACC 1 cut(s) 595
PspGI CCWGG 4 cut(s) 219, 610, 756, 773
PspN4I GGNNCC 4 cut(s) 145, 334, 783, 1044
PspPI GGNCC 3 cut(s) 584, 754, 952
PspPPI RGGWCCY 2 cut(s) 754, 952
PsuI RGATCY 1 cut(s) 332
PsyI GACNNNGTC 1 cut(s) 758
PvuII CAGCTG 1 cut(s) 511
RseI CAYNNNNRTG 1 cut(s) 161
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 3 cut(s) 563, 567, 993
Sau3AI GATC 2 cut(s) 230, 332
Sau96I GGNCC 3 cut(s) 584, 754, 952
SchI GAGTC 1 cut(s) 451
ScrFI CCNGG 5 cut(s) 221, 534, 612, 758, 775
SduI GDGCHC 1 cut(s) 494
SexAI ACCWGGT 2 cut(s) 219, 756
SfaNI GCATC 4 cut(s) 134, 235, 884, 900
SfuI TTCGAA 1 cut(s) 74
SinI GGWCC 2 cut(s) 754, 952
SmiMI CAYNNNNRTG 1 cut(s) 161
SseBI AGGCCT 1 cut(s) 1092
SsiI CCGC 3 cut(s) 248, 702, 910
SspMI CTAG 2 cut(s) 944, 1074
StuI AGGCCT 1 cut(s) 1092
StyD4I CCNGG 5 cut(s) 219, 532, 610, 756, 773
TaaI ACNGT 3 cut(s) 160, 301, 770
TaqI TCGA 2 cut(s) 74, 128
TfiI GAWTC 5 cut(s) 71, 83, 290, 383, 542
Tru1I TTAA 3 cut(s) 563, 567, 993
Tru9I TTAA 3 cut(s) 563, 567, 993
TscAI CASTG 2 cut(s) 163, 499
TseFI GTSAC 1 cut(s) 760
Tsp45I GTSAC 1 cut(s) 760
TspDTI ATGAA 1 cut(s) 44
TspGWI ACGGA 1 cut(s) 228
TspRI CASTG 2 cut(s) 163, 499
Tth111I GACNNNGTC 1 cut(s) 758
VneI GTGCAC 1 cut(s) 490
VpaK11BI GGWCC 2 cut(s) 754, 952
XapI RAATTY 3 cut(s) 10, 639, 790
XmnI GAANNNNTTC 1 cut(s) 541
XspI CTAG 2 cut(s) 944, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.