Rorug07G0019700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006624
Physical Location & Seq
Reverse (-)
1499703 .. 1502637
2935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0019700.1

Sequence Viewer

Length: 1353 bp
ATGGAGCCCTCCCCTGAATCCCGCGATAAAGTTGAAAAGTTTGCGAGGGCTACTGGGGATTTCCAGTCACTGTCAGAGGTTGATATTCGTCTTATTGCTATGACATATACACTGGAGGCTCAGATACATGGCACTGAGCGCCTCAGGGACTGTCCTCCGCCGGTGCATACTCTCAATGTCAGGAGGTTGCCGGAGAAGGAATTGCCCGGCTGGGAAAATAATGTCCCTAATTTGGAAGAGTGGGAAGCGCTGGAAAACAAAGCGGAGGATAAGAGCAACTCTGGTTCGAGGATTCTTCCGTTGAAAGATATTAACTTGAATGTTTCTGTTGATGGTTCTGCGGTGGAAATTAGAAGTGATGTTCATTCTGAGAATCAGGAGGGTGGTGAGGGCATTCAGGGGAGATATAGGAGATATTTTCCAAAGAAGAAAGAGATAAATATTGAAGGGAAGATGGTTTCTGATGGAATTGATGCGTCCCAAGGACAAGTTGATGATAATGCTGGTGACTGGATGCCTGCTGTTAGTCGGAGTACACATAGAAGGTTTTTGAGAAGAAAAGCTAGACGTGAGTCTTATGAGGCGGCATCAGCTGATAATGATCCTCAGCTACATGCTGAGGAAAACACAAGTGGTGGCGTACTTGTGGATGTGAATTTTTCTGTTGAAGGAGATGAGGTTGAAATGATAAATGAAAGGCTGGATCACTTGGAGATCTCAAGCGATAATGATGCAAGTGTTGATACATTAAATGTGAACGATGATACTAGTCAGCAGATCTGGATGCTGAGATCCTTGTCTGAGTCTAGTGTAGCTTGTATAACAAGTGATTTTGCAATGCAAAATGTTATTCTGCAGATGGGTTTACGGTTGTTGGCACCTGGAGGAATGCAGATCCGCCAGCTGAAGAGGTGGATTTTGAAATGCCATGCTTGTAATACTTTGACCACTGCAATTGGGAGAATGTTTTGTCCAAAGTGTGGAAATGGTGGTACCTTGTGCAAGGTAGCAGTTACAGTGGATGAGAATGGGATTACTGTTGCGGCACCTAAGCCTCAGATCACCAAGCGTGGCACAAGATTTTCACTTCCTTTACCACAAGGTGGAAGAAATGCCATTACCAAAAACCCTGTTTTACGTGAGGATCAGCTTCCACAAAAGTACCTCCATCCAAAGACAAAGAAGAAAGCAAATAAGCCGGGAGATGACGACCTACTCACTTCCAATGACTTCATCTTCATCCACCATTCGGATAAAAATGCTCCTTTGCAGCCTCCAGTGAGGAAGGCATTAGCTGCTTTCCGCGGGAGGAGGAATCCCAACGACAATCATTATTCACATTCCAAGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

450

Amino Acids

50.3

Weight (kDa)

6.38

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIN_6 PF17146 2 - 38 7.8e-10 PIN domain of ribonuclease
Zn_ribbon_NOB1 PF08772 299 - 369 1.3e-22 Nin one binding (NOB1) Zn-ribbon like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 992
AccB1I GGYRCC 3 cut(s) 877, 992, 1045
AccB7I CCANNNNNTGG 2 cut(s) 980, 1103
AccII CGCG 2 cut(s) 24, 1305
AciI CCGC 9 cut(s) 22, 158, 263, 341, 584, 898, 1043, 1303, 1305
AclWI GGATC 5 cut(s) 596, 711, 786, 889, 1152
AcsI RAATTY 1 cut(s) 655
AcuI CTGAAG 1 cut(s) 926
AdeI CACNNNGTG 1 cut(s) 1103
AfaI GTAC 4 cut(s) 535, 642, 994, 1163
AfeI AGCGCT 1 cut(s) 249
AfiI CCNNNNNNNGG 4 cut(s) 232, 980, 1103, 1249
AgsI TTSAA 7 cut(s) 35, 304, 319, 446, 668, 683, 922
AhlI ACTAGT 1 cut(s) 767
AjiI CACGTC 1 cut(s) 569
AjnI CCWGG 1 cut(s) 880
AluBI AGCT 7 cut(s) 563, 593, 610, 815, 904, 1150, 1295
AluI AGCT 7 cut(s) 563, 593, 610, 815, 904, 1150, 1295
AlwI GGATC 5 cut(s) 596, 711, 786, 889, 1152
AlwNI CAGNNNCTG 2 cut(s) 70, 150
Aor51HI AGCGCT 1 cut(s) 249
ApeKI GCWGC 2 cut(s) 1270, 1295
ApoI RAATTY 1 cut(s) 655
Asp718I GGTACC 1 cut(s) 992
AspLEI GCGC 2 cut(s) 141, 250
AsuC2I CCSGG 2 cut(s) 207, 1200
AsuHPI GGTGA 3 cut(s) 398, 518, 1054
AxyI CCTNAGG 1 cut(s) 143
BaeI ACNNNNGTAYC 2 cut(s) 1145, 1178
BanI GGYRCC 3 cut(s) 877, 992, 1045
BanII GRGCYC 1 cut(s) 9
BbvCI CCTCAGC 2 cut(s) 606, 618
BbvI GCAGC 2 cut(s) 1282, 1282
BccI CCATC 5 cut(s) 326, 448, 458, 853, 1176
BcgI CGANNNNNNTGC 2 cut(s) 713, 747
BciT130I CCWGG 1 cut(s) 882
BcnI CCSGG 2 cut(s) 207, 1200
BcuI ACTAGT 1 cut(s) 767
BfaI CTAG 3 cut(s) 564, 768, 807
BfmI CTRYAG 1 cut(s) 854
BfoI RGCGCY 2 cut(s) 142, 251
BglII AGATCT 2 cut(s) 714, 777
BisI GCNGC 4 cut(s) 585, 1044, 1271, 1296
BlsI GCNGC 4 cut(s) 586, 1045, 1272, 1297
Bme1390I CCNGG 3 cut(s) 207, 882, 1200
BmgBI CACGTC 1 cut(s) 569
BmiI GGNNCC 4 cut(s) 6, 879, 994, 1047
BmrFI CCNGG 3 cut(s) 207, 882, 1200
BmrI ACTGGG 1 cut(s) 63
BmsI GCATC 5 cut(s) 463, 504, 596, 721, 774
BmuI ACTGGG 1 cut(s) 63
BoxI GACNNNNGTC 1 cut(s) 571
BpmI CTGGAG 3 cut(s) 134, 903, 1260
Bpu10I CCTNAGC 3 cut(s) 606, 618, 1050
BpuEI CTTGAG 1 cut(s) 703
BpuMI CCSGG 2 cut(s) 207, 1200
BsaAI YACGTR 1 cut(s) 1139
BsaBI GATNNNNATC 1 cut(s) 600
BsaJI CCNNGG 2 cut(s) 481, 1303
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 4 cut(s) 232, 980, 1103, 1249
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 5 cut(s) 58, 64, 117, 515, 1277
Bse21I CCTNAGG 1 cut(s) 143
Bse3DI GCAATG 1 cut(s) 843
Bse8I GATNNNNATC 1 cut(s) 600
BseBI CCWGG 1 cut(s) 882
BseDI CCNNGG 2 cut(s) 481, 1303
BseGI GGATG 6 cut(s) 519, 655, 789, 1027, 1168, 1239
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 4 cut(s) 232, 980, 1103, 1249
BseMI GCAATG 1 cut(s) 843
BseMII CTCAG 9 cut(s) 126, 134, 157, 360, 609, 620, 779, 792, 1070
BseNI ACTGG 5 cut(s) 58, 64, 117, 515, 1277
BseRI GAGGAG 1 cut(s) 1324
BseXI GCAGC 2 cut(s) 1282, 1282
BseYI CCCAGC 1 cut(s) 210
Bsh1236I CGCG 2 cut(s) 24, 1305
BshNI GGYRCC 3 cut(s) 877, 992, 1045
BsiSI CCGG 4 cut(s) 161, 191, 207, 1199
BslFI GGGAC 3 cut(s) 161, 209, 463
BslI CCNNNNNNNGG 4 cut(s) 232, 980, 1103, 1249
BsmFI GGGAC 3 cut(s) 161, 209, 463
BsmI GAATGC 2 cut(s) 393, 894
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 8 cut(s) 601, 703, 714, 777, 791, 894, 1059, 1144
BspACI CCGC 9 cut(s) 22, 158, 263, 341, 584, 898, 1043, 1303, 1305
BspCNI CTCAG 9 cut(s) 127, 133, 156, 361, 610, 619, 780, 793, 1069
BspFNI CGCG 2 cut(s) 24, 1305
BspLI GGNNCC 4 cut(s) 6, 879, 994, 1047
BspMAI CTGCAG 1 cut(s) 858
BspPI GGATC 5 cut(s) 596, 711, 786, 889, 1152
BspT107I GGYRCC 3 cut(s) 877, 992, 1045
BsrDI GCAATG 1 cut(s) 843
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 5 cut(s) 58, 64, 117, 515, 1277
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 2 cut(s) 481, 1303
BssMI GATC 8 cut(s) 601, 703, 714, 777, 791, 894, 1059, 1144
BssT1I CCWWGG 1 cut(s) 481
Bst2UI CCWGG 1 cut(s) 882
Bst4CI ACNGT 5 cut(s) 72, 152, 870, 1018, 1039
Bst6I CTCTTC 2 cut(s) 231, 902
BstAPI GCANNNNNTGC 1 cut(s) 1295
BstBAI YACGTR 1 cut(s) 1139
BstC8I GCNNGC 2 cut(s) 519, 902
BstDSI CCRYGG 1 cut(s) 1303
BstF5I GGATG 6 cut(s) 519, 655, 789, 1027, 1168, 1239
BstFNI CGCG 2 cut(s) 24, 1305
BstH2I RGCGCY 2 cut(s) 142, 251
BstHHI GCGC 2 cut(s) 141, 250
BstKTI GATC 8 cut(s) 604, 706, 717, 780, 794, 897, 1062, 1147
BstMBI GATC 8 cut(s) 601, 703, 714, 777, 791, 894, 1059, 1144
BstMWI GCNNNNNNNGC 3 cut(s) 138, 590, 1295
BstNI CCWGG 1 cut(s) 882
BstNSI RCATGY 1 cut(s) 617
BstPAI GACNNNNGTC 1 cut(s) 571
BstSCI CCNGG 3 cut(s) 205, 880, 1198
BstSFI CTRYAG 1 cut(s) 854
BstUI CGCG 2 cut(s) 24, 1305
BstV1I GCAGC 2 cut(s) 1282, 1282
BstX2I RGATCY 4 cut(s) 714, 777, 791, 894
BstYI RGATCY 4 cut(s) 714, 777, 791, 894
Bsu36I CCTNAGG 1 cut(s) 143
BtgI CCRYGG 1 cut(s) 1303
BtrI CACGTC 1 cut(s) 569
BtsCI GGATG 6 cut(s) 519, 655, 789, 1027, 1168, 1239
BtsI GCAGTG 1 cut(s) 948
BtsIMutI CAGTG 6 cut(s) 68, 110, 132, 948, 1023, 1284
Cac8I GCNNGC 2 cut(s) 519, 902
CaiI CAGNNNCTG 2 cut(s) 70, 150
CfoI GCGC 2 cut(s) 141, 250
Cfr10I RCCGGY 1 cut(s) 160
Cfr42I CCGCGG 1 cut(s) 1306
CseI GACGC 1 cut(s) 465
Csp6I GTAC 4 cut(s) 534, 641, 993, 1162
CspCI CAANNNNNGTGG 2 cut(s) 937, 972
CviAII CATG 3 cut(s) 128, 614, 929
CviQI GTAC 4 cut(s) 534, 641, 993, 1162
DpnI GATC 8 cut(s) 603, 705, 716, 779, 793, 896, 1061, 1146
DpnII GATC 8 cut(s) 601, 703, 714, 777, 791, 894, 1059, 1144
DraIII CACNNNGTG 1 cut(s) 1103
Eam1104I CTCTTC 2 cut(s) 231, 902
EarI CTCTTC 2 cut(s) 231, 902
EciI GGCGGA 2 cut(s) 147, 887
Eco130I CCWWGG 1 cut(s) 481
Eco24I GRGCYC 1 cut(s) 9
Eco47III AGCGCT 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 926
Eco81I CCTNAGG 1 cut(s) 143
EcoRII CCWGG 1 cut(s) 880
EcoT14I CCWWGG 1 cut(s) 481
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 481
FaeI CATG 3 cut(s) 131, 617, 932
FaqI GGGAC 3 cut(s) 161, 209, 463
FatI CATG 3 cut(s) 127, 613, 928
FauI CCCGC 2 cut(s) 29, 1298
Fnu4HI GCNGC 4 cut(s) 585, 1044, 1271, 1296
FokI GGATG 6 cut(s) 526, 662, 796, 1034, 1155, 1226
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 4 cut(s) 585, 1044, 1271, 1296
FspBI CTAG 3 cut(s) 564, 768, 807
GlaI GCGC 2 cut(s) 140, 249
GluI GCNGC 4 cut(s) 585, 1044, 1271, 1296
GsaI CCCAGC 1 cut(s) 214
GsuI CTGGAG 3 cut(s) 134, 903, 1260
HaeII RGCGCY 2 cut(s) 142, 251
HapII CCGG 4 cut(s) 161, 191, 207, 1199
HgaI GACGC 1 cut(s) 465
HhaI GCGC 2 cut(s) 141, 250
Hin1II CATG 3 cut(s) 131, 617, 932
Hin6I GCGC 2 cut(s) 139, 248
HinP1I GCGC 2 cut(s) 139, 248
HinfI GANTC 6 cut(s) 17, 292, 373, 572, 803, 1315
HpaII CCGG 4 cut(s) 161, 191, 207, 1199
HphI GGTGA 3 cut(s) 398, 518, 1054
Hpy166II GTNNAC 3 cut(s) 536, 757, 866
Hpy188I TCNGA 8 cut(s) 76, 123, 370, 463, 531, 802, 1059, 1252
Hpy188III TCNNGA 3 cut(s) 181, 377, 781
Hpy8I GTNNAC 3 cut(s) 536, 757, 866
HpyAV CCTTC 5 cut(s) 190, 440, 537, 662, 1279
HpyCH4III ACNGT 5 cut(s) 72, 152, 870, 1018, 1039
HpyCH4IV ACGT 2 cut(s) 568, 1138
HpyCH4V TGCA 9 cut(s) 166, 734, 836, 841, 856, 892, 953, 1002, 1270
HpyF10VI GCNNNNNNNGC 3 cut(s) 138, 590, 1295
HpySE526I ACGT 2 cut(s) 568, 1138
Hsp92II CATG 3 cut(s) 131, 617, 932
HspAI GCGC 2 cut(s) 139, 248
KpnI GGTACC 1 cut(s) 996
KspI CCGCGG 1 cut(s) 1306
Kzo9I GATC 8 cut(s) 601, 703, 714, 777, 791, 894, 1059, 1144
LmnI GCTCC 2 cut(s) 4, 1267
Lsp1109I GCAGC 2 cut(s) 1282, 1282
LweI GCATC 5 cut(s) 463, 504, 596, 721, 774
MaeI CTAG 3 cut(s) 564, 768, 807
MaeII ACGT 2 cut(s) 568, 1138
MaeIII GTNAC 3 cut(s) 66, 506, 1012
MalI GATC 8 cut(s) 603, 705, 716, 779, 793, 896, 1061, 1146
MboI GATC 8 cut(s) 601, 703, 714, 777, 791, 894, 1059, 1144
MboII GAAGA 9 cut(s) 248, 287, 439, 463, 567, 919, 1119, 1195, 1228
MfeI CAATTG 1 cut(s) 954
MflI RGATCY 4 cut(s) 714, 777, 791, 894
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 6 cut(s) 200, 229, 348, 468, 655, 954
MlyI GAGTC 2 cut(s) 581, 812
MmeI TCCRAC 1 cut(s) 509
MseI TTAA 2 cut(s) 312, 749
MspA1I CMGCKG 3 cut(s) 593, 904, 1305
MspI CCGG 4 cut(s) 161, 191, 207, 1199
MspR9I CCNGG 3 cut(s) 207, 882, 1200
MunI CAATTG 1 cut(s) 954
Mva1269I GAATGC 2 cut(s) 393, 894
MvaI CCWGG 1 cut(s) 882
MvnI CGCG 2 cut(s) 24, 1305
MwoI GCNNNNNNNGC 3 cut(s) 138, 590, 1295
NciI CCSGG 2 cut(s) 207, 1200
NdeII GATC 8 cut(s) 601, 703, 714, 777, 791, 894, 1059, 1144
NlaIII CATG 3 cut(s) 131, 617, 932
NlaIV GGNNCC 4 cut(s) 6, 879, 994, 1047
NmuCI GTSAC 2 cut(s) 66, 506
NspI RCATGY 1 cut(s) 617
PctI GAATGC 2 cut(s) 393, 894
PfeI GAWTC 4 cut(s) 17, 292, 373, 1315
PflMI CCANNNNNTGG 2 cut(s) 980, 1103
PkrI GCNGC 4 cut(s) 586, 1045, 1272, 1297
PleI GAGTC 2 cut(s) 580, 811
PpsI GAGTC 2 cut(s) 580, 811
Ppu21I YACGTR 1 cut(s) 1139
PshAI GACNNNNGTC 1 cut(s) 571
Psp6I CCWGG 1 cut(s) 880
PspFI CCCAGC 1 cut(s) 210
PspGI CCWGG 1 cut(s) 880
PspN4I GGNNCC 4 cut(s) 6, 879, 994, 1047
PstI CTGCAG 1 cut(s) 858
PstNI CAGNNNCTG 2 cut(s) 70, 150
PsuI RGATCY 4 cut(s) 714, 777, 791, 894
PvuII CAGCTG 2 cut(s) 593, 904
RsaI GTAC 4 cut(s) 535, 642, 994, 1163
RsaNI GTAC 4 cut(s) 534, 641, 993, 1162
SacII CCGCGG 1 cut(s) 1306
SaqAI TTAA 2 cut(s) 312, 749
SatI GCNGC 4 cut(s) 585, 1044, 1271, 1296
Sau3AI GATC 8 cut(s) 601, 703, 714, 777, 791, 894, 1059, 1144
SchI GAGTC 2 cut(s) 581, 812
ScrFI CCNGG 3 cut(s) 207, 882, 1200
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 5 cut(s) 463, 504, 596, 721, 774
SfcI CTRYAG 1 cut(s) 854
Sfr303I CCGCGG 1 cut(s) 1306
SgrAI CRCCGGYG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 1306
SmlI CTYRAG 1 cut(s) 718
SmoI CTYRAG 1 cut(s) 718
SpeI ACTAGT 1 cut(s) 767
Sse9I AATT 6 cut(s) 200, 229, 348, 468, 655, 954
SsiI CCGC 9 cut(s) 22, 158, 263, 341, 584, 898, 1043, 1303, 1305
SspI AATATT 1 cut(s) 442
SspMI CTAG 3 cut(s) 564, 768, 807
StyD4I CCNGG 3 cut(s) 205, 880, 1198
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 5 cut(s) 72, 152, 870, 1018, 1039
TaiI ACGT 2 cut(s) 571, 1141
TaqI TCGA 1 cut(s) 287
TasI AATT 6 cut(s) 200, 229, 348, 468, 655, 954
TatI WGTACW 1 cut(s) 533
TauI GCSGC 2 cut(s) 587, 1046
TfiI GAWTC 4 cut(s) 17, 292, 373, 1315
Tru1I TTAA 2 cut(s) 312, 749
Tru9I TTAA 2 cut(s) 312, 749
TscAI CASTG 6 cut(s) 75, 117, 139, 955, 1023, 1284
TseFI GTSAC 2 cut(s) 66, 506
TseI GCWGC 2 cut(s) 1270, 1295
Tsp45I GTSAC 2 cut(s) 66, 506
TspDTI ATGAA 4 cut(s) 353, 708, 1222, 1228
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 6 cut(s) 75, 117, 139, 955, 1023, 1284
Van91I CCANNNNNTGG 2 cut(s) 980, 1103
XapI RAATTY 1 cut(s) 655
XceI RCATGY 1 cut(s) 617
XspI CTAG 3 cut(s) 564, 768, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.