RLG00000004152

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006624
Physical Location & Seq
Forward (+)
56626881 .. 56629408
2528 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004152

Sequence Viewer

Length: 1104 bp
ATGATCCGCCAAAGCAACCGGGTCCTTGAACTCCGGTCCTTCGCTTCTTTAGTCCCTCGAGTCTTTGACTCTTCATCGTCTTCGTTGACCTCTATGGATCCGAATGTTACAGTAACATCCAAGAGTTCAGATAGTTCGGATGATAAGAAGGTCGTGTCGAATTTAGTTTCAAATGTTAAAGAAGTTCAAGATGTGAAGAAAGATAAAGTTCAGGATGGTGGATCCAAGGATGGTGTTGGGAATGGCAAGGAGAAGAGCGGTTCAGATGGGGAAGTTGGGTCAACAGAATCTCATAGTGTGCCAAAGGTTAAGAAAGGATCAAATGATGGGAAGAATGGAAAGCCGAGTGAGAAATCAAAGGCAATGCCTAAGGAGGAGGTGGGTAATGCCGGAAATGTGAACCCGGTGAGAGAGGACGGTACTGCCAGTGAAGAATGTGGTTCAGCTAATATGTGCACTGTTGAGGAGAACAAGCTGGTTGCGTGTTTGAGAGTTCCTGGAGATGATGATTCTCCTCATCTTTCACTTTTAATTCAGAACAAGGGTAAGGACCCTCTTGTGGTTACCATTTCAGCTCCTGATTTTGTGCGGCTAGACAAATCAGAAGTTCAACTGAAAGAGAAAGATAATGCAAAGGTGGATGTTTCTGTTGGAAGTGGAGGAGCTACCAGTATAATTGTTCTAAAGGCAGGAAATGGCAATTGCAGCCTTGATTTCAAGGACCTAATCACGCACAGTTCCAGGAAGGAGCCTGATAATTCTTCAAGTACCACCTACTTATACCTCTGGACACACAGACCTGCCATTGGGATTCTATTGGTTGCCTCACTGATGATTTTGGTATTGGCTGGGATGTACGTCCGATTTATGAAGAGGCGGGTTTCCAGCAGTGGCTTTAAATATCAGAAGCTAGATGATGTGCGCCTCCCAGTTTTGAGCAGCGAAAAGCCGGAGTTGCACATTAATGATGGATGGGACGACACTTGGGATGATAACTGGGATGATGAGGAGGCCCCCCATACACCCTCGATGCCGGTTACTCCTAGCCTCTCTGGCAAAGGCCTTGCCTCACGAAGGTTGAACAAGGAAGGGTGGAAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

368

Amino Acids

39.69

Weight (kDa)

5.97

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7356 PF24053 139 - 242 7e-31 Domain of unknown function (DUF7356)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 808
AccBSI CCGCTC 1 cut(s) 258
AciI CCGC 4 cut(s) 7, 258, 589, 877
AclWI GGATC 5 cut(s) 92, 105, 216, 229, 325
AcsI RAATTY 1 cut(s) 160
AfaI GTAC 3 cut(s) 421, 769, 857
AfiI CCNNNNNNNGG 3 cut(s) 559, 806, 1074
AgsI TTSAA 7 cut(s) 29, 171, 188, 611, 718, 765, 1081
AjnI CCWGG 2 cut(s) 496, 740
AluBI AGCT 5 cut(s) 446, 475, 575, 665, 910
AluI AGCT 5 cut(s) 446, 475, 575, 665, 910
Alw21I GWGCWC 1 cut(s) 458
Alw44I GTGCAC 1 cut(s) 454
AlwI GGATC 5 cut(s) 92, 105, 216, 229, 325
AlwNI CAGNNNCTG 1 cut(s) 578
Ama87I CYCGRG 1 cut(s) 57
AoxI GGCC 2 cut(s) 1011, 1060
ApaLI GTGCAC 1 cut(s) 454
ApeKI GCWGC 2 cut(s) 705, 939
ApoI RAATTY 1 cut(s) 160
AseI ATTAAT 1 cut(s) 963
AspLEI GCGC 1 cut(s) 924
AspS9I GGNCC 5 cut(s) 22, 36, 550, 721, 1012
AsuC2I CCSGG 2 cut(s) 20, 404
AsuHPI GGTGA 1 cut(s) 418
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 4 cut(s) 22, 36, 550, 721
AxyI CCTNAGG 1 cut(s) 369
BaeGI GKGCMC 1 cut(s) 458
BamHI GGATCC 2 cut(s) 97, 221
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 458
BbvI GCAGC 2 cut(s) 717, 951
BccI CCATC 6 cut(s) 209, 224, 260, 320, 962, 966
BciT130I CCWGG 2 cut(s) 498, 742
BcnI CCSGG 2 cut(s) 20, 404
BfaI CTAG 3 cut(s) 593, 911, 1044
BfuAI ACCTGC 1 cut(s) 808
BglI GCCNNNNNGGC 1 cut(s) 1053
BisI GCNGC 3 cut(s) 590, 706, 940
BlsI GCNGC 3 cut(s) 591, 707, 941
Bme1390I CCNGG 4 cut(s) 20, 404, 498, 742
Bme18I GGWCC 4 cut(s) 22, 36, 550, 721
BmeT110I CYCGRG 1 cut(s) 57
BmgT120I GGNCC 5 cut(s) 22, 36, 550, 721, 1012
BmiI GGNNCC 6 cut(s) 23, 99, 223, 552, 750, 1014
BmrFI CCNGG 4 cut(s) 20, 404, 498, 742
BmrI ACTGGG 2 cut(s) 923, 1006
BmsI GCATC 1 cut(s) 1020
BmuI ACTGGG 2 cut(s) 923, 1006
BpiI GAAGAC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 519
BpuMI CCSGG 2 cut(s) 20, 404
BsaJI CCNNGG 1 cut(s) 225
BsaWI WCCGGW 1 cut(s) 33
Bsc4I CCNNNNNNNGG 3 cut(s) 559, 806, 1074
Bse118I RCCGGY 1 cut(s) 1033
Bse1I ACTGG 4 cut(s) 426, 669, 929, 1001
Bse21I CCTNAGG 1 cut(s) 369
Bse3DI GCAATG 1 cut(s) 369
BseBI CCWGG 2 cut(s) 498, 742
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 9 cut(s) 116, 145, 220, 235, 646, 858, 977, 994, 1006
BseLI CCNNNNNNNGG 3 cut(s) 559, 806, 1074
BseMI GCAATG 1 cut(s) 369
BseNI ACTGG 4 cut(s) 426, 669, 929, 1001
BseRI GAGGAG 5 cut(s) 389, 479, 504, 675, 1022
BseSI GKGCMC 1 cut(s) 458
BseXI GCAGC 2 cut(s) 717, 951
BseYI CCCAGC 1 cut(s) 848
BshFI GGCC 2 cut(s) 1013, 1062
BsiHKAI GWGCWC 1 cut(s) 458
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 6 cut(s) 19, 34, 390, 404, 950, 1034
BslFI GGGAC 2 cut(s) 38, 989
BslI CCNNNNNNNGG 3 cut(s) 559, 806, 1074
BsmFI GGGAC 2 cut(s) 38, 989
BsnI GGCC 2 cut(s) 1013, 1062
BsoBI CYCGRG 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 458
Bsp143I GATC 4 cut(s) 3, 97, 221, 317
BspACI CCGC 4 cut(s) 7, 258, 589, 877
BspANI GGCC 2 cut(s) 1013, 1062
BspLI GGNNCC 6 cut(s) 23, 99, 223, 552, 750, 1014
BspMI ACCTGC 1 cut(s) 808
BspPI GGATC 5 cut(s) 92, 105, 216, 229, 325
BspQI GCTCTTC 1 cut(s) 248
BsrBI CCGCTC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 1033
BsrI ACTGG 4 cut(s) 426, 669, 929, 1001
BssAI RCCGGY 1 cut(s) 1033
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 4 cut(s) 3, 97, 221, 317
BssT1I CCWWGG 1 cut(s) 225
Bst2UI CCWGG 2 cut(s) 498, 742
Bst4CI ACNGT 4 cut(s) 112, 419, 460, 737
Bst6I CTCTTC 3 cut(s) 76, 248, 866
BstDEI CTNAG 1 cut(s) 369
BstEII GGTNACC 1 cut(s) 562
BstENI CCTNNNNNAGG 1 cut(s) 1072
BstF5I GGATG 9 cut(s) 116, 145, 220, 235, 646, 858, 977, 994, 1006
BstHHI GCGC 1 cut(s) 924
BstKTI GATC 4 cut(s) 6, 100, 224, 320
BstMBI GATC 4 cut(s) 3, 97, 221, 317
BstMWI GCNNNNNNNGC 3 cut(s) 705, 955, 1053
BstNI CCWGG 2 cut(s) 498, 742
BstPI GGTNACC 1 cut(s) 562
BstSCI CCNGG 4 cut(s) 18, 402, 496, 740
BstSLI GKGCMC 1 cut(s) 458
BstV1I GCAGC 2 cut(s) 717, 951
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 2 cut(s) 97, 221
BstYI RGATCY 2 cut(s) 97, 221
Bsu36I CCTNAGG 1 cut(s) 369
BsuRI GGCC 2 cut(s) 1013, 1062
BtsCI GGATG 9 cut(s) 116, 145, 220, 235, 646, 858, 977, 994, 1006
BtsI GCAGTG 1 cut(s) 895
BtsIMutI CAGTG 4 cut(s) 433, 456, 827, 895
BveI ACCTGC 1 cut(s) 808
CaiI CAGNNNCTG 1 cut(s) 578
CfoI GCGC 1 cut(s) 924
Cfr10I RCCGGY 1 cut(s) 1033
Cfr13I GGNCC 5 cut(s) 22, 36, 550, 721, 1012
Csp6I GTAC 3 cut(s) 420, 768, 856
CviQI GTAC 3 cut(s) 420, 768, 856
DdeI CTNAG 1 cut(s) 369
DpnI GATC 4 cut(s) 5, 99, 223, 319
DpnII GATC 4 cut(s) 3, 97, 221, 317
DraI TTTAAA 1 cut(s) 898
Eam1104I CTCTTC 3 cut(s) 76, 248, 866
EarI CTCTTC 3 cut(s) 76, 248, 866
Eco130I CCWWGG 1 cut(s) 225
Eco147I AGGCCT 1 cut(s) 1062
Eco47I GGWCC 4 cut(s) 22, 36, 550, 721
Eco81I CCTNAGG 1 cut(s) 369
Eco88I CYCGRG 1 cut(s) 57
Eco91I GGTNACC 1 cut(s) 562
EcoNI CCTNNNNNAGG 1 cut(s) 1072
EcoO109I RGGNCCY 4 cut(s) 22, 550, 721, 1012
EcoO65I GGTNACC 1 cut(s) 562
EcoRII CCWGG 2 cut(s) 496, 740
EcoT14I CCWWGG 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 225
FaiI YATR 7 cut(s) 95, 294, 452, 674, 781, 869, 1020
FaqI GGGAC 2 cut(s) 38, 989
FauI CCCGC 1 cut(s) 870
Fnu4HI GCNGC 3 cut(s) 590, 706, 940
FokI GGATG 9 cut(s) 103, 152, 227, 242, 653, 865, 984, 1001, 1013
Fsp4HI GCNGC 3 cut(s) 590, 706, 940
FspBI CTAG 3 cut(s) 593, 911, 1044
GlaI GCGC 1 cut(s) 923
GluI GCNGC 3 cut(s) 590, 706, 940
GsaI CCCAGC 1 cut(s) 852
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 2 cut(s) 1013, 1062
HapII CCGG 6 cut(s) 19, 34, 390, 404, 950, 1034
HhaI GCGC 1 cut(s) 924
Hin6I GCGC 1 cut(s) 922
HinP1I GCGC 1 cut(s) 922
HincII GTYRAC 2 cut(s) 87, 282
HindII GTYRAC 2 cut(s) 87, 282
HinfI GANTC 5 cut(s) 60, 68, 287, 509, 811
HpaII CCGG 6 cut(s) 19, 34, 390, 404, 950, 1034
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 4 cut(s) 87, 282, 400, 456
Hpy188I TCNGA 8 cut(s) 102, 130, 139, 265, 537, 604, 863, 906
Hpy188III TCNNGA 5 cut(s) 188, 212, 578, 787, 1071
Hpy8I GTNNAC 4 cut(s) 87, 282, 400, 456
HpyAV CCTTC 5 cut(s) 49, 142, 739, 1068, 1082
HpyCH4III ACNGT 4 cut(s) 112, 419, 460, 737
HpyCH4IV ACGT 1 cut(s) 858
HpyCH4V TGCA 4 cut(s) 456, 632, 705, 958
HpyF10VI GCNNNNNNNGC 3 cut(s) 705, 955, 1053
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 1 cut(s) 858
HspAI GCGC 1 cut(s) 922
Kzo9I GATC 4 cut(s) 3, 97, 221, 317
LguI GCTCTTC 1 cut(s) 248
LmnI GCTCC 3 cut(s) 580, 662, 748
Lsp1109I GCAGC 2 cut(s) 717, 951
LweI GCATC 1 cut(s) 1020
MaeI CTAG 3 cut(s) 593, 911, 1044
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 4 cut(s) 106, 112, 562, 1036
MalI GATC 4 cut(s) 5, 99, 223, 319
MbiI CCGCTC 1 cut(s) 258
MboI GATC 4 cut(s) 3, 97, 221, 317
MboII GAAGA 8 cut(s) 63, 72, 208, 265, 343, 443, 753, 883
MfeI CAATTG 1 cut(s) 700
MflI RGATCY 2 cut(s) 97, 221
MhlI GDGCHC 1 cut(s) 458
MluCI AATT 5 cut(s) 160, 531, 675, 700, 757
MlyI GAGTC 2 cut(s) 62, 69
MmeI TCCRAC 1 cut(s) 631
MseI TTAA 5 cut(s) 177, 309, 530, 897, 963
MslI CAYNNNNRTG 1 cut(s) 963
MspI CCGG 6 cut(s) 19, 34, 390, 404, 950, 1034
MspR9I CCNGG 4 cut(s) 20, 404, 498, 742
MunI CAATTG 1 cut(s) 700
MvaI CCWGG 2 cut(s) 498, 742
MwoI GCNNNNNNNGC 3 cut(s) 705, 955, 1053
NciI CCSGG 2 cut(s) 20, 404
NdeII GATC 4 cut(s) 3, 97, 221, 317
NlaIV GGNNCC 6 cut(s) 23, 99, 223, 552, 750, 1014
NmeAIII GCCGAG 1 cut(s) 369
PaeR7I CTCGAG 1 cut(s) 57
PceI AGGCCT 1 cut(s) 1062
PciSI GCTCTTC 1 cut(s) 248
PfeI GAWTC 3 cut(s) 287, 509, 811
PfoI TCCNGGA 2 cut(s) 496, 740
PkrI GCNGC 3 cut(s) 591, 707, 941
PleI GAGTC 2 cut(s) 62, 68
PpsI GAGTC 2 cut(s) 62, 68
PpuMI RGGWCCY 3 cut(s) 22, 550, 721
PshBI ATTAAT 1 cut(s) 963
Psp5II RGGWCCY 3 cut(s) 22, 550, 721
Psp6I CCWGG 2 cut(s) 496, 740
PspEI GGTNACC 1 cut(s) 562
PspFI CCCAGC 1 cut(s) 848
PspGI CCWGG 2 cut(s) 496, 740
PspN4I GGNNCC 6 cut(s) 23, 99, 223, 552, 750, 1014
PspPI GGNCC 5 cut(s) 22, 36, 550, 721, 1012
PspPPI RGGWCCY 3 cut(s) 22, 550, 721
PspXI VCTCGAGB 1 cut(s) 57
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 2 cut(s) 97, 221
RsaI GTAC 3 cut(s) 421, 769, 857
RsaNI GTAC 3 cut(s) 420, 768, 856
RseI CAYNNNNRTG 1 cut(s) 963
SapI GCTCTTC 1 cut(s) 248
SaqAI TTAA 5 cut(s) 177, 309, 530, 897, 963
SatI GCNGC 3 cut(s) 590, 706, 940
Sau3AI GATC 4 cut(s) 3, 97, 221, 317
Sau96I GGNCC 5 cut(s) 22, 36, 550, 721, 1012
SchI GAGTC 2 cut(s) 62, 69
ScrFI CCNGG 4 cut(s) 20, 404, 498, 742
SduI GDGCHC 1 cut(s) 458
SfaNI GCATC 1 cut(s) 1020
Sfr274I CTCGAG 1 cut(s) 57
SinI GGWCC 4 cut(s) 22, 36, 550, 721
SlaI CTCGAG 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 963
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
Sse9I AATT 5 cut(s) 160, 531, 675, 700, 757
SseBI AGGCCT 1 cut(s) 1062
SsiI CCGC 4 cut(s) 7, 258, 589, 877
SspMI CTAG 3 cut(s) 593, 911, 1044
StuI AGGCCT 1 cut(s) 1062
StyD4I CCNGG 4 cut(s) 18, 402, 496, 740
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 4 cut(s) 112, 419, 460, 737
TaiI ACGT 1 cut(s) 861
TaqI TCGA 3 cut(s) 58, 158, 1028
TasI AATT 5 cut(s) 160, 531, 675, 700, 757
TauI GCSGC 1 cut(s) 592
TfiI GAWTC 3 cut(s) 287, 509, 811
Tru1I TTAA 5 cut(s) 177, 309, 530, 897, 963
Tru9I TTAA 5 cut(s) 177, 309, 530, 897, 963
TscAI CASTG 4 cut(s) 433, 463, 834, 895
TseI GCWGC 2 cut(s) 705, 939
TspDTI ATGAA 2 cut(s) 63, 884
TspRI CASTG 4 cut(s) 433, 463, 834, 895
VneI GTGCAC 1 cut(s) 454
VpaK11BI GGWCC 4 cut(s) 22, 36, 550, 721
VspI ATTAAT 1 cut(s) 963
XagI CCTNNNNNAGG 1 cut(s) 1072
XapI RAATTY 1 cut(s) 160
XhoI CTCGAG 1 cut(s) 57
XspI CTAG 3 cut(s) 593, 911, 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.