MD14G1127900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006624
Physical Location & Seq
Forward (+)
20359958 .. 20361638
1681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1127900.v1.1.491

Sequence Viewer

Length: 537 bp
ATGAACATGGCCAAAGGCCCTCTCTTGGTTACCATTGTAACTCCTGATTTTGTGCAACTGGACGAAACAAAAATTCAACTTGAAGAGAAAGAAAACAGAAAAGGAGGGGCTGGCAGTTCAATTGTCCTAAAAGCTAGAAATGGTCGATGCAACCTTGATTTGAAGGACCTAATCACAAACAGTTCCAGGAAGAAGCCTGAGAATTCTTCAAACTTCGCCTACACAAACTTCCCAAAACAAAAACCTACGATTGCAATTCTGTTCTTTGCTTCAGCAATGATATTAGCATCGGCTTGGATGTGCATCAGCTTTAGAAATAGGCGGGTCTCTGGCAGTGGGTTGAAATATCAAAAGCTAGATAAGGACCTACCAATTTCCAATGGAAAAAACCGGGTGTTGCATGTTAACGATGTATGGGATAATAACTGGGACGATAATTGGGATGATGAGGAGGCACCTCACACACCCTTGATGCCGATTACTCCTAGTCTTTCTGGCAAGGGCCTCGCCTCACGACGGTTGAACAAGGAAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.94

Weight (kDa)

9.39

Isoelectric Point (pI)

25.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7356 PF24053 2 - 57 2.4e-07 Domain of unknown function (DUF7356)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 454
AciI CCGC 1 cut(s) 322
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 2 cut(s) 72, 202
AcuI CTGAAG 1 cut(s) 255
AfiI CCNNNNNNNGG 2 cut(s) 25, 516
AgsI TTSAA 7 cut(s) 77, 83, 120, 163, 210, 343, 523
AjnI CCWGG 1 cut(s) 185
AluBI AGCT 3 cut(s) 134, 309, 355
AluI AGCT 3 cut(s) 134, 309, 355
Alw26I GTCTC 1 cut(s) 331
AoxI GGCC 3 cut(s) 9, 16, 502
ApoI RAATTY 2 cut(s) 72, 202
AspS9I GGNCC 4 cut(s) 17, 166, 364, 502
AsuC2I CCSGG 1 cut(s) 392
AvaII GGWCC 2 cut(s) 166, 364
BalI TGGCCA 1 cut(s) 11
BanI GGYRCC 1 cut(s) 454
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BciT130I CCWGG 1 cut(s) 187
BcnI CCSGG 1 cut(s) 392
BcoDI GTCTC 1 cut(s) 331
BfaI CTAG 3 cut(s) 135, 356, 486
Bme1390I CCNGG 2 cut(s) 187, 392
Bme18I GGWCC 2 cut(s) 166, 364
BmgT120I GGNCC 4 cut(s) 17, 166, 364, 502
BmiI GGNNCC 1 cut(s) 456
BmrFI CCNGG 2 cut(s) 187, 392
BmrI ACTGGG 1 cut(s) 436
BmsI GCATC 4 cut(s) 137, 296, 312, 462
BmuI ACTGGG 1 cut(s) 436
BpuMI CCSGG 1 cut(s) 392
BsaBI GATNNNNATC 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 331
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 516
Bse1I ACTGG 2 cut(s) 63, 431
Bse3DI GCAATG 1 cut(s) 282
Bse8I GATNNNNATC 1 cut(s) 302
BseBI CCWGG 1 cut(s) 187
BseGI GGATG 2 cut(s) 303, 448
BseJI GATNNNNATC 1 cut(s) 302
BseLI CCNNNNNNNGG 2 cut(s) 25, 516
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 2 cut(s) 63, 431
BseRI GAGGAG 1 cut(s) 464
BshFI GGCC 3 cut(s) 11, 18, 504
BshNI GGYRCC 1 cut(s) 454
BsiSI CCGG 1 cut(s) 391
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 2 cut(s) 25, 516
BsmAI GTCTC 1 cut(s) 331
BsmFI GGGAC 1 cut(s) 443
BsnI GGCC 3 cut(s) 11, 18, 504
Bso31I GGTCTC 1 cut(s) 331
BspACI CCGC 1 cut(s) 322
BspANI GGCC 3 cut(s) 11, 18, 504
BspCNI CTCAG 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 456
BspT107I GGYRCC 1 cut(s) 454
BspTNI GGTCTC 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 282
BsrI ACTGG 2 cut(s) 63, 431
Bst2UI CCWGG 1 cut(s) 187
Bst4CI ACNGT 2 cut(s) 182, 519
Bst6I CTCTTC 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 1 cut(s) 198
BstEII GGTNACC 1 cut(s) 28
BstF5I GGATG 2 cut(s) 303, 448
BstMAI GTCTC 1 cut(s) 331
BstNI CCWGG 1 cut(s) 187
BstNSI RCATGY 1 cut(s) 404
BstPI GGTNACC 1 cut(s) 28
BstSCI CCNGG 2 cut(s) 185, 390
BsuRI GGCC 3 cut(s) 11, 18, 504
BtsCI GGATG 2 cut(s) 303, 448
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 112
Cfr13I GGNCC 4 cut(s) 17, 166, 364, 502
CviAII CATG 2 cut(s) 7, 401
CviJI RGCY 9 cut(s) 11, 18, 110, 134, 196, 293, 309, 355, 504
CviKI_1 RGCY 9 cut(s) 11, 18, 110, 134, 196, 293, 309, 355, 504
DdeI CTNAG 1 cut(s) 198
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 331
Eco47I GGWCC 2 cut(s) 166, 364
Eco57I CTGAAG 1 cut(s) 255
Eco91I GGTNACC 1 cut(s) 28
EcoO109I RGGNCCY 4 cut(s) 17, 166, 364, 502
EcoO65I GGTNACC 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 185
FaeI CATG 2 cut(s) 10, 404
FaiI YATR 3 cut(s) 8, 402, 415
FaqI GGGAC 1 cut(s) 443
FatI CATG 2 cut(s) 6, 400
FauI CCCGC 1 cut(s) 315
FokI GGATG 2 cut(s) 310, 455
FspBI CTAG 3 cut(s) 135, 356, 486
HaeIII GGCC 3 cut(s) 11, 18, 504
HapII CCGG 1 cut(s) 391
Hin1II CATG 2 cut(s) 10, 404
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HpaI GTTAAC 1 cut(s) 406
HpaII CCGG 1 cut(s) 391
Hpy166II GTNNAC 1 cut(s) 406
Hpy188III TCNNGA 2 cut(s) 44, 513
Hpy8I GTNNAC 1 cut(s) 406
Hpy99I CGWCG 1 cut(s) 519
HpyAV CCTTC 1 cut(s) 157
HpyCH4III ACNGT 2 cut(s) 182, 519
HpyCH4V TGCA 5 cut(s) 55, 150, 254, 303, 400
HpyF3I CTNAG 1 cut(s) 198
Hsp92II CATG 2 cut(s) 10, 404
KspAI GTTAAC 1 cut(s) 406
LweI GCATC 4 cut(s) 137, 296, 312, 462
MaeI CTAG 3 cut(s) 135, 356, 486
MaeIII GTNAC 2 cut(s) 28, 37
MboII GAAGA 3 cut(s) 95, 198, 202
MfeI CAATTG 1 cut(s) 120
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 6 cut(s) 72, 120, 202, 255, 372, 436
MluNI TGGCCA 1 cut(s) 11
MnlI CCTC 7 cut(s) 30, 98, 442, 445, 468, 515, 520
Mox20I TGGCCA 1 cut(s) 11
MscI TGGCCA 1 cut(s) 11
MseI TTAA 1 cut(s) 405
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 1 cut(s) 391
MspR9I CCNGG 2 cut(s) 187, 392
MunI CAATTG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 187
NciI CCSGG 1 cut(s) 392
NlaIII CATG 2 cut(s) 10, 404
NlaIV GGNNCC 1 cut(s) 456
NspI RCATGY 1 cut(s) 404
PfoI TCCNGGA 1 cut(s) 185
PpuMI RGGWCCY 2 cut(s) 166, 364
Psp5II RGGWCCY 2 cut(s) 166, 364
Psp6I CCWGG 1 cut(s) 185
PspEI GGTNACC 1 cut(s) 28
PspGI CCWGG 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 456
PspPI GGNCC 4 cut(s) 17, 166, 364, 502
PspPPI RGGWCCY 2 cut(s) 166, 364
SaqAI TTAA 1 cut(s) 405
Sau96I GGNCC 4 cut(s) 17, 166, 364, 502
ScrFI CCNGG 2 cut(s) 187, 392
SetI ASST 8 cut(s) 136, 156, 171, 247, 311, 357, 369, 460
SfaNI GCATC 4 cut(s) 137, 296, 312, 462
SinI GGWCC 2 cut(s) 166, 364
Sse9I AATT 6 cut(s) 72, 120, 202, 255, 372, 436
SsiI CCGC 1 cut(s) 322
SspMI CTAG 3 cut(s) 135, 356, 486
StyD4I CCNGG 2 cut(s) 185, 390
TaaI ACNGT 2 cut(s) 182, 519
TaqI TCGA 1 cut(s) 145
TasI AATT 6 cut(s) 72, 120, 202, 255, 372, 436
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 1 cut(s) 340
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 340
VpaK11BI GGWCC 2 cut(s) 166, 364
XapI RAATTY 2 cut(s) 72, 202
XceI RCATGY 1 cut(s) 404
XspI CTAG 3 cut(s) 135, 356, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.