FvH4_5g09080

DUF4210

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001019
Physical Location & Seq
Forward (+)
5240600 .. 5246280
5681 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g09080.t4

Sequence Viewer

Length: 1620 bp
ATGGGCTTGCCTCAAGTGTCTTCTAGCAGTATTGCTGAGGAAGTGGCAGCATCACTATGCACTTTTATGCAACCTCCATCCAGAATTGCTGGCGTGAGTAGCTGTGATTTAAGTGGTTTGCATGGGGGAAACATGGGAAATCACATGCAGGATATGACATGCTCTTATTCTGGAGAGCTCCAAAGGAACACCATGACAGAGATTCCAAATAAACCAGATTTTTCGAATATGTGTACTGGTCGATCAAACATGCATTCCTTGAAGATCAACATCATGGAACAAAATAACTGGTTGAGTCAGAAAGTAGGTCCAAATGTTCAGACATCTATTCCCAGAATTGTTGGTTTTGAACCAAGAAACTTTAATTCTCCTCTTGATTTGTTCAATGCAAACCGACATTCTTCTAGTGGGGTGGCTGTCACAGGCGACACAAATGAGGCCACTGGGTTAGTAGCCAGGAAACGGTTATCGTCTCCTTTGAATGGAATGCATCTCCAAGATCAATTTAATGGCAATTCTCTAAACATTGGTGATGGGATCCATAAGACCTTTGGATGCGAAGATGGCTCTAATGTTCCCACATTTCAAGAGCATAAAAAAGCTCACATTGGCAACTCCAGTTGTTTCGGTTCTACTATTTGGTCTGCATCTTGTTCCCCAGAGTGGAAGTATTCATTGGATGAGAAGTGTACTATAAACTCCTCCTTTGTAGTTGATGGTCCTTTGCTTGAAACCAAGGAGCCAAAATCACATAATCAGTTCATATCATCATCTGGACTTGATCACTCCCCAGAAACGATAAAGGTAAGGTCACAGTCTGGAGCAATAGCCATAAATTCAAGAAAGGTGGCGCTACATCCACTTTCTTTATCTCCTCTTGGCCCAAAATTTCCTGAAAGAGTGAAATTTGGAGAACTCTGCGGGGATCATACACCAAAGGCAGATGATGATTACATTATGACTTTGAAGAACATGGACCCTTCACTCGATGGGACTATCCCAGGCATGTCAGCTTCTTGGAAAGAGGAGACTTTTAGGGCTCCAAGTAATTCATTGGAAGATGTCGATATTTTGCAGAAAAAGTTTGTCCTCTTTAGTCCTGAGAGTCCCAACGGGATGCAACAGCAATGGAATCAGGATTTGAAATCCAAGTCGGAAGCTGTTAAATTAGCTCGAAGCCTCACTGGACTGCCTGTTAGGAGGTCCTTGGTGGGTTCATTTGAGGAATCCCTCTTGTCTGGCCGACTGGTATCAGCAAAGGTTAATCAGAGAATTGATGGTTTTCTAGCTGTTCTGAATGTTACTGGAGGAAATTTCTCCCCAAAATCACAAAAACTTCCATTTGCCGTGACCAGTGTGGATGGAGAGAACTACTTGCTGTATTATTCATCAATAGATCTTGCTGGGAGAAGGACATCAGAAATGTATGGAGGACCCAAGATGAAAAGAAGCCTTAGCATGGATGATTCACAAGCAGACAGGAACCGTCTACGAATACCTATGAAAGGCCGCATACAACTGGTTCTCAGCAATCCAGAGAAGACACCAATTCACACATTCATCTGTAACTACGACTTGAGTGACATGCCAACCGGTAGCAAGGTAATCAGGAAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromosome_seg DUF4210

Protein Analysis

540

Amino Acids

59.08

Weight (kDa)

8.23

Isoelectric Point (pI)

52.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromosome_seg PF13889 658 - 740 3.9e-24 Chromosome segregation during meiosis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1489
AciI CCGC 2 cut(s) 921, 1510
AclWI GGATC 3 cut(s) 532, 545, 933
AcoI YGGCCR 1 cut(s) 1240
AcsI RAATTY 4 cut(s) 835, 887, 905, 1312
AfaI GTAC 3 cut(s) 235, 691, 1616
AfiI CCNNNNNNNGG 5 cut(s) 462, 482, 663, 1459, 1505
AgeI ACCGGT 1 cut(s) 1592
AgsI TTSAA 9 cut(s) 262, 350, 385, 481, 587, 731, 840, 967, 1144
AjnI CCWGG 2 cut(s) 455, 1000
AjuI GAANNNNNNNTTGG 2 cut(s) 659, 691
AluBI AGCT 7 cut(s) 102, 178, 602, 1013, 1160, 1172, 1289
AluI AGCT 7 cut(s) 102, 178, 602, 1013, 1160, 1172, 1289
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 2 cut(s) 477, 1022
AlwI GGATC 3 cut(s) 532, 545, 933
AoxI GGCC 4 cut(s) 438, 880, 1240, 1507
ApeKI GCWGC 1 cut(s) 47
ApoI RAATTY 4 cut(s) 835, 887, 905, 1312
AsiGI ACCGGT 1 cut(s) 1592
AspLEI GCGC 1 cut(s) 853
AspS9I GGNCC 6 cut(s) 308, 719, 881, 976, 1203, 1433
AsuHPI GGTGA 1 cut(s) 542
AsuII TTCGAA 1 cut(s) 224
AvaII GGWCC 5 cut(s) 308, 719, 976, 1203, 1433
BamHI GGATCC 1 cut(s) 537
BanII GRGCYC 2 cut(s) 180, 1042
BbsI GAAGAC 2 cut(s) 12, 1547
Bbv12I GWGCWC 1 cut(s) 180
BbvCI CCTCAGC 1 cut(s) 36
BbvI GCAGC 1 cut(s) 59
BccI CCATC 7 cut(s) 85, 527, 557, 710, 983, 1271, 1355
BceAI ACGGC 1 cut(s) 1331
BciT130I CCWGG 2 cut(s) 457, 1002
BclI TGATCA 1 cut(s) 781
BcoDI GTCTC 2 cut(s) 477, 1022
BfaI CTAG 3 cut(s) 24, 405, 1286
BfoI RGCGCY 1 cut(s) 854
BglII AGATCT 1 cut(s) 1396
BisI GCNGC 2 cut(s) 48, 1510
BlsI GCNGC 2 cut(s) 49, 1511
BmcAI AGTACT 1 cut(s) 1616
Bme1390I CCNGG 2 cut(s) 457, 1002
Bme18I GGWCC 5 cut(s) 308, 719, 976, 1203, 1433
BmgT120I GGNCC 6 cut(s) 308, 719, 881, 976, 1203, 1433
BmiI GGNNCC 6 cut(s) 539, 741, 978, 1041, 1435, 1484
BmrFI CCNGG 2 cut(s) 457, 1002
BmrI ACTGGG 1 cut(s) 453
BmsI GCATC 5 cut(s) 59, 499, 545, 656, 1107
BmuI ACTGGG 1 cut(s) 453
BpiI GAAGAC 2 cut(s) 12, 1547
BpmI CTGGAG 4 cut(s) 192, 601, 840, 1326
Bpu10I CCTNAGC 2 cut(s) 36, 1454
Bpu14I TTCGAA 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 1597
BsaBI GATNNNNATC 1 cut(s) 269
BsaJI CCNNGG 3 cut(s) 735, 1000, 1206
BsaWI WCCGGW 1 cut(s) 1592
Bsc4I CCNNNNNNNGG 5 cut(s) 462, 482, 663, 1459, 1505
Bse118I RCCGGY 1 cut(s) 1592
Bse1I ACTGG 9 cut(s) 241, 293, 448, 618, 1189, 1251, 1309, 1353, 1524
Bse3DI GCAATG 1 cut(s) 1133
Bse8I GATNNNNATC 1 cut(s) 269
BseBI CCWGG 2 cut(s) 457, 1002
BseDI CCNNGG 3 cut(s) 735, 1000, 1206
BseGI GGATG 7 cut(s) 77, 560, 685, 856, 1122, 1366, 1468
BseJI GATNNNNATC 1 cut(s) 269
BseLI CCNNNNNNNGG 5 cut(s) 462, 482, 663, 1459, 1505
BseMI GCAATG 1 cut(s) 1133
BseMII CTCAG 3 cut(s) 27, 1092, 1540
BseNI ACTGG 9 cut(s) 241, 293, 448, 618, 1189, 1251, 1309, 1353, 1524
BseRI GAGGAG 4 cut(s) 360, 691, 864, 1040
BseXI GCAGC 1 cut(s) 59
BseYI CCCAGC 1 cut(s) 1403
BshFI GGCC 4 cut(s) 440, 882, 1242, 1509
BshTI ACCGGT 1 cut(s) 1592
BsiHKAI GWGCWC 1 cut(s) 180
BsiSI CCGG 1 cut(s) 1593
BslFI GGGAC 2 cut(s) 1006, 1092
BslI CCNNNNNNNGG 5 cut(s) 462, 482, 663, 1459, 1505
BsmAI GTCTC 2 cut(s) 477, 1022
BsmBI CGTCTC 1 cut(s) 477
BsmFI GGGAC 2 cut(s) 1006, 1092
BsmI GAATGC 2 cut(s) 253, 492
BsnI GGCC 4 cut(s) 440, 882, 1242, 1509
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 2 cut(s) 180, 1042
Bsp143I GATC 7 cut(s) 242, 264, 499, 537, 781, 925, 1396
BspACI CCGC 2 cut(s) 921, 1510
BspANI GGCC 4 cut(s) 440, 882, 1242, 1509
BspCNI CTCAG 3 cut(s) 28, 1093, 1539
BspLI GGNNCC 6 cut(s) 539, 741, 978, 1041, 1435, 1484
BspPI GGATC 3 cut(s) 532, 545, 933
BspT104I TTCGAA 1 cut(s) 224
BsrDI GCAATG 1 cut(s) 1133
BsrFI RCCGGY 1 cut(s) 1592
BsrI ACTGG 9 cut(s) 241, 293, 448, 618, 1189, 1251, 1309, 1353, 1524
BssAI RCCGGY 1 cut(s) 1592
BssECI CCNNGG 3 cut(s) 735, 1000, 1206
BssMI GATC 7 cut(s) 242, 264, 499, 537, 781, 925, 1396
BssT1I CCWWGG 2 cut(s) 735, 1206
Bst2UI CCWGG 2 cut(s) 457, 1002
Bst4CI ACNGT 3 cut(s) 465, 816, 1487
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 8, 91
BstDEI CTNAG 4 cut(s) 36, 1101, 1454, 1526
BstENI CCTNNNNNAGG 1 cut(s) 1503
BstF5I GGATG 7 cut(s) 77, 560, 685, 856, 1122, 1366, 1468
BstH2I RGCGCY 1 cut(s) 854
BstHHI GCGC 1 cut(s) 853
BstKTI GATC 7 cut(s) 245, 267, 502, 540, 784, 928, 1399
BstMAI GTCTC 2 cut(s) 477, 1022
BstMBI GATC 7 cut(s) 242, 264, 499, 537, 781, 925, 1396
BstMWI GCNNNNNNNGC 2 cut(s) 99, 564
BstNI CCWGG 2 cut(s) 457, 1002
BstNSI RCATGY 5 cut(s) 148, 162, 253, 1009, 1588
BstSCI CCNGG 2 cut(s) 455, 1000
BstV1I GCAGC 1 cut(s) 59
BstV2I GAAGAC 2 cut(s) 12, 1547
BstX2I RGATCY 2 cut(s) 537, 1396
BstYI RGATCY 2 cut(s) 537, 1396
BsuRI GGCC 4 cut(s) 440, 882, 1242, 1509
BtsCI GGATG 7 cut(s) 77, 560, 685, 856, 1122, 1366, 1468
BtsIMutI CAGTG 3 cut(s) 441, 1182, 1360
Cac8I GCNNGC 2 cut(s) 8, 91
CfoI GCGC 1 cut(s) 853
Cfr10I RCCGGY 1 cut(s) 1592
Cfr13I GGNCC 6 cut(s) 308, 719, 881, 976, 1203, 1433
Csp6I GTAC 3 cut(s) 234, 690, 1615
CspAI ACCGGT 1 cut(s) 1592
CspCI CAANNNNNGTGG 2 cut(s) 828, 863
CviQI GTAC 3 cut(s) 234, 690, 1615
DdeI CTNAG 4 cut(s) 36, 1101, 1454, 1526
DpnI GATC 7 cut(s) 244, 266, 501, 539, 783, 927, 1398
DpnII GATC 7 cut(s) 242, 264, 499, 537, 781, 925, 1396
EaeI YGGCCR 1 cut(s) 1240
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 2 cut(s) 735, 1206
Eco24I GRGCYC 2 cut(s) 180, 1042
Eco47I GGWCC 5 cut(s) 308, 719, 976, 1203, 1433
Eco53kI GAGCTC 1 cut(s) 178
EcoICRI GAGCTC 1 cut(s) 178
EcoNI CCTNNNNNAGG 1 cut(s) 1503
EcoO109I RGGNCCY 2 cut(s) 1203, 1433
EcoRII CCWGG 2 cut(s) 455, 1000
EcoT14I CCWWGG 2 cut(s) 735, 1206
EcoT22I ATGCAT 2 cut(s) 255, 492
EcoT38I GRGCYC 2 cut(s) 180, 1042
ErhI CCWWGG 2 cut(s) 735, 1206
Esp3I CGTCTC 1 cut(s) 477
FaqI GGGAC 2 cut(s) 1006, 1092
FauI CCCGC 1 cut(s) 914
FbaI TGATCA 1 cut(s) 781
FblI GTMKAC 1 cut(s) 1489
Fnu4HI GCNGC 2 cut(s) 48, 1510
FokI GGATG 7 cut(s) 64, 567, 692, 843, 1129, 1373, 1475
FriOI GRGCYC 2 cut(s) 180, 1042
Fsp4HI GCNGC 2 cut(s) 48, 1510
FspBI CTAG 3 cut(s) 24, 405, 1286
GlaI GCGC 1 cut(s) 852
GluI GCNGC 2 cut(s) 48, 1510
GsaI CCCAGC 1 cut(s) 1407
GsuI CTGGAG 4 cut(s) 192, 601, 840, 1326
HaeII RGCGCY 1 cut(s) 854
HaeIII GGCC 4 cut(s) 440, 882, 1242, 1509
HapII CCGG 1 cut(s) 1593
HhaI GCGC 1 cut(s) 853
Hin6I GCGC 1 cut(s) 851
HinP1I GCGC 1 cut(s) 851
HinfI GANTC 6 cut(s) 202, 295, 1105, 1132, 1226, 1466
HpaII CCGG 1 cut(s) 1593
HphI GGTGA 1 cut(s) 542
Hpy166II GTNNAC 3 cut(s) 234, 690, 1490
Hpy188I TCNGA 6 cut(s) 300, 321, 1156, 1269, 1296, 1420
Hpy8I GTNNAC 3 cut(s) 234, 690, 1490
HpyAV CCTTC 2 cut(s) 990, 1404
HpyCH4III ACNGT 3 cut(s) 465, 816, 1487
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 564
HpyF3I CTNAG 4 cut(s) 36, 1101, 1454, 1526
HspAI GCGC 1 cut(s) 851
Ksp22I TGATCA 1 cut(s) 781
Kzo9I GATC 7 cut(s) 242, 264, 499, 537, 781, 925, 1396
LmnI GCTCC 4 cut(s) 183, 739, 821, 1045
Lsp1109I GCAGC 1 cut(s) 59
LweI GCATC 5 cut(s) 59, 499, 545, 656, 1107
MaeI CTAG 3 cut(s) 24, 405, 1286
MaeIII GTNAC 6 cut(s) 418, 810, 1300, 1348, 1565, 1580
MalI GATC 7 cut(s) 244, 266, 501, 539, 783, 927, 1398
MboI GATC 7 cut(s) 242, 264, 499, 537, 781, 925, 1396
MboII GAAGA 7 cut(s) 12, 274, 393, 572, 979, 1070, 1552
MflI RGATCY 2 cut(s) 537, 1396
MhlI GDGCHC 2 cut(s) 180, 1042
MlyI GAGTC 2 cut(s) 304, 1114
MmeI TCCRAC 1 cut(s) 1134
Mph1103I ATGCAT 2 cut(s) 255, 492
MseI TTAA 5 cut(s) 110, 363, 507, 1164, 1263
MslI CAYNNNNRTG 2 cut(s) 55, 65
MspI CCGG 1 cut(s) 1593
MspR9I CCNGG 2 cut(s) 457, 1002
Mva1269I GAATGC 2 cut(s) 253, 492
MvaI CCWGG 2 cut(s) 457, 1002
MwoI GCNNNNNNNGC 2 cut(s) 99, 564
NdeII GATC 7 cut(s) 242, 264, 499, 537, 781, 925, 1396
NlaIV GGNNCC 6 cut(s) 539, 741, 978, 1041, 1435, 1484
NmuCI GTSAC 4 cut(s) 418, 810, 1348, 1580
NsiI ATGCAT 2 cut(s) 255, 492
NspI RCATGY 5 cut(s) 148, 162, 253, 1009, 1588
NspV TTCGAA 1 cut(s) 224
PctI GAATGC 2 cut(s) 253, 492
PfeI GAWTC 4 cut(s) 202, 1132, 1226, 1466
PinAI ACCGGT 1 cut(s) 1592
PkrI GCNGC 2 cut(s) 49, 1511
PleI GAGTC 2 cut(s) 303, 1113
PpsI GAGTC 2 cut(s) 303, 1113
PpuMI RGGWCCY 2 cut(s) 1203, 1433
Psp124BI GAGCTC 1 cut(s) 180
Psp5II RGGWCCY 2 cut(s) 1203, 1433
Psp6I CCWGG 2 cut(s) 455, 1000
PspFI CCCAGC 1 cut(s) 1403
PspGI CCWGG 2 cut(s) 455, 1000
PspN4I GGNNCC 6 cut(s) 539, 741, 978, 1041, 1435, 1484
PspPI GGNCC 6 cut(s) 308, 719, 881, 976, 1203, 1433
PspPPI RGGWCCY 2 cut(s) 1203, 1433
PsuI RGATCY 2 cut(s) 537, 1396
RsaI GTAC 3 cut(s) 235, 691, 1616
RsaNI GTAC 3 cut(s) 234, 690, 1615
RseI CAYNNNNRTG 2 cut(s) 55, 65
SacI GAGCTC 1 cut(s) 180
SaqAI TTAA 5 cut(s) 110, 363, 507, 1164, 1263
SatI GCNGC 2 cut(s) 48, 1510
Sau3AI GATC 7 cut(s) 242, 264, 499, 537, 781, 925, 1396
Sau96I GGNCC 6 cut(s) 308, 719, 881, 976, 1203, 1433
ScaI AGTACT 1 cut(s) 1616
SchI GAGTC 2 cut(s) 304, 1114
ScrFI CCNGG 2 cut(s) 457, 1002
SduI GDGCHC 2 cut(s) 180, 1042
SfaNI GCATC 5 cut(s) 59, 499, 545, 656, 1107
SfuI TTCGAA 1 cut(s) 224
SinI GGWCC 5 cut(s) 308, 719, 976, 1203, 1433
SmiMI CAYNNNNRTG 2 cut(s) 55, 65
SmlI CTYRAG 2 cut(s) 12, 1576
SmoI CTYRAG 2 cut(s) 12, 1576
SsiI CCGC 2 cut(s) 921, 1510
SspMI CTAG 3 cut(s) 24, 405, 1286
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 455, 1000
StyI CCWWGG 2 cut(s) 735, 1206
TaaI ACNGT 3 cut(s) 465, 816, 1487
TaqI TCGA 5 cut(s) 224, 241, 987, 1065, 1174
TatI WGTACW 3 cut(s) 233, 689, 1614
TauI GCSGC 1 cut(s) 1512
TfiI GAWTC 4 cut(s) 202, 1132, 1226, 1466
Tru1I TTAA 5 cut(s) 110, 363, 507, 1164, 1263
Tru9I TTAA 5 cut(s) 110, 363, 507, 1164, 1263
TscAI CASTG 3 cut(s) 448, 1189, 1360
TseFI GTSAC 4 cut(s) 418, 810, 1348, 1580
TseI GCWGC 1 cut(s) 47
Tsp45I GTSAC 4 cut(s) 418, 810, 1348, 1580
TspDTI ATGAA 8 cut(s) 663, 751, 1041, 1206, 1377, 1457, 1517, 1549
TspRI CASTG 3 cut(s) 448, 1189, 1360
VpaK11BI GGWCC 5 cut(s) 308, 719, 976, 1203, 1433
XagI CCTNNNNNAGG 1 cut(s) 1503
XapI RAATTY 4 cut(s) 835, 887, 905, 1312
XceI RCATGY 5 cut(s) 148, 162, 253, 1009, 1588
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 1489
XspI CTAG 3 cut(s) 24, 405, 1286
ZrmI AGTACT 1 cut(s) 1616
Zsp2I ATGCAT 2 cut(s) 255, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.