MD06G1134000.v1.1

DUF4210

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001019
Physical Location & Seq
Reverse (-)
27824236 .. 27830044
5809 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1134000.v1.1.491

Sequence Viewer

Length: 2196 bp
ATGGGATTGCCTCAAGTGTCTTCTAGCAGCGTTGCCGAGGAAGTGGCAGCATCACTGTGCACATTTATGCAACCTCAACCTAGAGTTGCTGGCATGAGCACTTGTGATTTCAGTGGGATGCACGGAGCAAACCTGGGAAACCATATGCAGGTGGATTTCACATGCTCTTCTTTTGGAGAATTCCATAGGAAATCCATCACGGAGATTCCAAATAAACCAGAGTTTTCTAATTCTCGTAAAGATAGTAGTAGATCAAGCATGCATGGATTGAAGATTAACTCTTCAGAACAAAAAAACTGGTTAAGTCAGAGATGTGGGCAGAATATTCAGACACCTGTTCCTAGGGTTGTTGGTTTTGAACCAAGAATATTAGATTCCCCTATTAATTTCTTTAATGGAGACCAATACTCTTCAAGTGCGTTGACCGTCACAGGGGACACAACTGAGGCTGCTGGGTCAGTACTCAGGAGGCGGTTATTGTCTCCTTTGAATGGAATGCTTCTCCAGGATCAGTGTAATGGTGATTCTCTAGAGATTGTGGACAGGGTTCATAAGAGTGGCTCCTGGGGTTGTAATGCTAGTTCTAATGATCCCATGTTGCAAGAGCATAAGAAAGCTCATCTTGGCAACTCCAGTTGTTGCAATTCTACAATCTTGTCTGCATCTTGTTCCCTGGAGTGGAAGAGATCACCGGATCACAACTGCAATACAAACTCCTCTTTTGTATTTGATGGTCCTTTGCTTGAAAACAAGGAGTCACGATCTCCGAATCAATTGATATCGTCATCCGGACTTGATTACTCTAGAGAAACAATTAAGGTGAGGTCACAATCAAGCGCTGTAGACATATCTTCAAGTAAAGTTGTGTTGCCTACACTTTCTTTATCTCCTCTTGGTCCAAAGTTTCCTGAAAGAGTTAAACCTGGAGAATCATGCAGGGTTCGTACACCAAAGTTAGATAACAATATAACCCTGAAGGACATGGAACAGTCACTTAATGGAACGATTGCAGGCATTTCCTCATCCTGGAAAGAGGACGCTTTTAGGTGGCTAAGGAAATCATCAGAAGATGTTGATGTTCTGCAGGAAAAGTTTGACCTCTTTAGTTCTGAGAGTACTACTCCTGGGATGGGGCAGCACTGGAGTCAGGACTTGAAACTTAATCCTAAATGTGTTAAATTAGTACGATCACCGACTGGATTGCCTGTTAGAAGGTCATTGGTTGGTTCATTTGAGGAATCCCTGTTGTCTGGTCGATTATTATCTACAAAGAGAATTGATGGTTTTCTTGCTGTTCTGAATGTCACTGGGGGGAATTTCTCGCCAAAACCTCAGAAACTTCCATTTTCAGTGACCAGTGTGGATGGAGATAACTATCTACTATATTATTCATCAATCGGTCTTGCTGAAAGTACAACACCAAACAAGTATGGAAGCCCCAGGATGAAGAGGAGCCTTAGCATGGATGATTCACGGGCAAACAAGAACCGTCTGAGAATACCTACGAAAGGCCGTATACAACTGGTTCTCAGCAATCCAGAGAAGACACCTATTCACACCTTCTTCTGTAATTATGATTTGAATGACATGCCAGCTGGAACCAAGACGTTTTTGCGCCAAAAGATCACTCTAGCCTCATCTGAAACAACCTCTAAGGCCGGGAATGGAAGACATGCAGATCCTGCTAAAGAAAATGATGCCAAACCATCTTCGATCCTAGATGCTGCGAATTCTTTGCAGCTTAGTGACAATGATGCGGACTCAAACGAGTTTAAGGATGTAGTTAATCAGTACCAAAGAGAGGGAGAACGCACAAAGGCCTGTTATCACAGTGAACCCAGTGATTGTAGCCTTTGTACGTCTGAAAAGACCAGTGAGATTAGTGAAAGTAGGGTGCGTAGCCCTTTAAAGACGAGCAAGAAAACTGCTGGTGCTGGCGTTCTGCGTTATGCTCTTCACCTTCGTTTCTCATGCCTATTTCCTAAGAAATGTTCGAGGTCAGTCCAGAGATGCAAGTTAGATCCATCATCTGGACAAGCGACAAACAGTATCGATGTCGAAGGGGGGAGGCGCTTCTACTTGTATAATGATTTGAGGGTCGTGTTCCCTCAACGCCAGACGGATGCTGATGAGGGCAAGTTACATGTGGAATATCACTTTCCTTCCGATCCGAAATACTTCGATATCAGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromosome_seg DUF4210

Protein Analysis

732

Amino Acids

80.7

Weight (kDa)

8.75

Isoelectric Point (pI)

59.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromosome_seg PF13889 647 - 729 1.1e-22 Chromosome segregation during meiosis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
Acc36I ACCTGC 1 cut(s) 139
AccB7I CCANNNNNTGG 1 cut(s) 2030
AccI GTMKAC 2 cut(s) 843, 1516
AccIII TCCGGA 1 cut(s) 788
AciI CCGC 2 cut(s) 472, 1757
AclWI GGATC 7 cut(s) 516, 584, 702, 1673, 1708, 2015, 2162
AcsI RAATTY 3 cut(s) 179, 1315, 1729
AcuI CTGAAG 2 cut(s) 267, 995
AfaI GTAC 7 cut(s) 462, 946, 1117, 1185, 1414, 1793, 1858
AfeI AGCGCT 1 cut(s) 838
AflIII ACRYGT 1 cut(s) 2143
AgsI TTSAA 8 cut(s) 271, 359, 414, 490, 746, 855, 1156, 1582
AjnI CCWGG 8 cut(s) 132, 504, 563, 672, 922, 1025, 1123, 1439
AluBI AGCT 3 cut(s) 617, 1595, 1741
AluI AGCT 3 cut(s) 617, 1595, 1741
Alw21I GWGCWC 2 cut(s) 62, 101
Alw26I GTCTC 2 cut(s) 393, 486
Alw44I GTGCAC 1 cut(s) 58
AlwI GGATC 7 cut(s) 516, 584, 702, 1673, 1708, 2015, 2162
AlwNI CAGNNNCTG 1 cut(s) 1682
Aor13HI TCCGGA 1 cut(s) 788
Aor51HI AGCGCT 1 cut(s) 838
AoxI GGCC 3 cut(s) 1510, 1656, 1818
ApaLI GTGCAC 1 cut(s) 58
ApeKI GCWGC 6 cut(s) 27, 47, 449, 1135, 1724, 1738
ApoI RAATTY 3 cut(s) 179, 1315, 1729
AseI ATTAAT 1 cut(s) 384
Asp700I GAANNNNTTC 1 cut(s) 2177
AspA2I CCTAGG 1 cut(s) 341
AspLEI GCGC 3 cut(s) 839, 1617, 2073
AspS9I GGNCC 2 cut(s) 734, 896
AsuC2I CCSGG 1 cut(s) 1660
AsuHPI GGTGA 5 cut(s) 533, 681, 832, 1182, 1949
AvaII GGWCC 2 cut(s) 734, 896
AvrII CCTAGG 1 cut(s) 341
BaeGI GKGCMC 1 cut(s) 62
BbsI GAAGAC 3 cut(s) 12, 1550, 1675
Bbv12I GWGCWC 2 cut(s) 62, 101
BbvI GCAGC 6 cut(s) 39, 59, 436, 1147, 1711, 1750
BccI CCATC 7 cut(s) 203, 725, 1123, 1274, 1358, 1714, 2032
BceAI ACGGC 1 cut(s) 1497
BcgI CGANNNNNNTGC 4 cut(s) 1183, 1217, 1717, 1751
BciT130I CCWGG 8 cut(s) 134, 506, 565, 674, 924, 1027, 1125, 1441
BcnI CCSGG 1 cut(s) 1660
BcoDI GTCTC 2 cut(s) 393, 486
BfaI CTAG 8 cut(s) 24, 81, 342, 530, 579, 804, 1631, 1718
BfmI CTRYAG 2 cut(s) 840, 1082
BfoI RGCGCY 2 cut(s) 840, 2074
BfuAI ACCTGC 1 cut(s) 139
BisI GCNGC 6 cut(s) 28, 48, 450, 1136, 1725, 1739
BlnI CCTAGG 1 cut(s) 341
BlsI GCNGC 6 cut(s) 29, 49, 451, 1137, 1726, 1740
BmcAI AGTACT 2 cut(s) 462, 1117
Bme1390I CCNGG 9 cut(s) 134, 506, 565, 674, 924, 1027, 1125, 1441, 1660
Bme18I GGWCC 2 cut(s) 734, 896
BmgT120I GGNCC 2 cut(s) 734, 896
BmiI GGNNCC 3 cut(s) 562, 1454, 1600
BmrFI CCNGG 9 cut(s) 134, 506, 565, 674, 924, 1027, 1125, 1441, 1660
BmrI ACTGGG 2 cut(s) 1317, 1833
BmsI GCATC 8 cut(s) 59, 108, 671, 1687, 1711, 1744, 2000, 2113
BmuI ACTGGG 2 cut(s) 1317, 1833
BpiI GAAGAC 3 cut(s) 12, 1550, 1675
BplI GAGNNNNNCTC 2 cut(s) 1105, 1137
BpmI CTGGAG 5 cut(s) 488, 616, 695, 945, 1162
Bpu10I CCTNAGC 2 cut(s) 1052, 1457
BpuMI CCSGG 1 cut(s) 1660
Bsa29I ATCGAT 1 cut(s) 2052
BsaBI GATNNNNATC 2 cut(s) 1261, 1374
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 7 cut(s) 36, 133, 341, 564, 672, 1124, 1439
BsaWI WCCGGW 2 cut(s) 691, 788
Bse1I ACTGG 9 cut(s) 302, 633, 1145, 1201, 1312, 1356, 1527, 1839, 1872
Bse8I GATNNNNATC 2 cut(s) 1261, 1374
BseAI TCCGGA 1 cut(s) 788
BseBI CCWGG 8 cut(s) 134, 506, 565, 674, 924, 1027, 1125, 1441
BseCI ATCGAT 1 cut(s) 2052
BseDI CCNNGG 7 cut(s) 36, 133, 341, 564, 672, 1124, 1439
BseGI GGATG 9 cut(s) 123, 785, 1022, 1134, 1369, 1449, 1471, 1783, 2128
BseJI GATNNNNATC 2 cut(s) 1261, 1374
BseMII CTCAG 6 cut(s) 435, 478, 1101, 1346, 1484, 1543
BseNI ACTGG 9 cut(s) 302, 633, 1145, 1201, 1312, 1356, 1527, 1839, 1872
BseRI GAGGAG 3 cut(s) 706, 879, 1465
BseSI GKGCMC 1 cut(s) 62
BseXI GCAGC 6 cut(s) 39, 59, 436, 1147, 1711, 1750
BseYI CCCAGC 1 cut(s) 452
BshFI GGCC 3 cut(s) 1512, 1658, 1820
BshVI ATCGAT 1 cut(s) 2052
BsiHKAI GWGCWC 2 cut(s) 62, 101
BsiSI CCGG 3 cut(s) 692, 789, 1659
BslFI GGGAC 1 cut(s) 449
BsmAI GTCTC 2 cut(s) 393, 486
BsmFI GGGAC 1 cut(s) 449
BsmI GAATGC 1 cut(s) 501
BsnI GGCC 3 cut(s) 1512, 1658, 1820
Bso31I GGTCTC 1 cut(s) 393
Bsp1286I GDGCHC 2 cut(s) 62, 101
Bsp13I TCCGGA 1 cut(s) 788
BspACI CCGC 2 cut(s) 472, 1757
BspANI GGCC 3 cut(s) 1512, 1658, 1820
BspCNI CTCAG 6 cut(s) 436, 477, 1102, 1345, 1485, 1542
BspDI ATCGAT 1 cut(s) 2052
BspEI TCCGGA 1 cut(s) 788
BspLI GGNNCC 3 cut(s) 562, 1454, 1600
BspMAI CTGCAG 1 cut(s) 1086
BspMI ACCTGC 1 cut(s) 139
BspPI GGATC 7 cut(s) 516, 584, 702, 1673, 1708, 2015, 2162
BspQI GCTCTTC 2 cut(s) 172, 1959
BspTNI GGTCTC 1 cut(s) 393
BsrI ACTGG 9 cut(s) 302, 633, 1145, 1201, 1312, 1356, 1527, 1839, 1872
BssECI CCNNGG 7 cut(s) 36, 133, 341, 564, 672, 1124, 1439
BssNAI GTATAC 1 cut(s) 1517
BssT1I CCWWGG 1 cut(s) 341
Bst1107I GTATAC 1 cut(s) 1517
Bst2UI CCWGG 8 cut(s) 134, 506, 565, 674, 924, 1027, 1125, 1441
Bst4CI ACNGT 6 cut(s) 57, 427, 990, 1490, 1832, 2048
Bst6I CTCTTC 6 cut(s) 172, 286, 415, 677, 1442, 1959
BstAPI GCANNNNNTGC 1 cut(s) 1682
BstC8I GCNNGC 5 cut(s) 91, 260, 1012, 1593, 1936
BstENI CCTNNNNNAGG 1 cut(s) 1506
BstF5I GGATG 9 cut(s) 123, 785, 1022, 1134, 1369, 1449, 1471, 1783, 2128
BstH2I RGCGCY 2 cut(s) 840, 2074
BstHHI GCGC 3 cut(s) 839, 1617, 2073
BstMAI GTCTC 2 cut(s) 393, 486
BstMWI GCNNNNNNNGC 1 cut(s) 1682
BstNI CCWGG 8 cut(s) 134, 506, 565, 674, 924, 1027, 1125, 1441
BstNSI RCATGY 5 cut(s) 165, 262, 1591, 1676, 2147
BstSCI CCNGG 9 cut(s) 132, 504, 563, 672, 922, 1025, 1123, 1439, 1658
BstSFI CTRYAG 2 cut(s) 840, 1082
BstSLI GKGCMC 1 cut(s) 62
BstV1I GCAGC 6 cut(s) 39, 59, 436, 1147, 1711, 1750
BstV2I GAAGAC 3 cut(s) 12, 1550, 1675
BstX2I RGATCY 2 cut(s) 1678, 2020
BstYI RGATCY 2 cut(s) 1678, 2020
BstZ17I GTATAC 1 cut(s) 1517
Bsu15I ATCGAT 1 cut(s) 2052
BsuRI GGCC 3 cut(s) 1512, 1658, 1820
BsuTUI ATCGAT 1 cut(s) 2052
BtsCI GGATG 9 cut(s) 123, 785, 1022, 1134, 1369, 1449, 1471, 1783, 2128
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 5 cut(s) 91, 260, 1012, 1593, 1936
CaiI CAGNNNCTG 1 cut(s) 1682
CfoI GCGC 3 cut(s) 839, 1617, 2073
Cfr13I GGNCC 2 cut(s) 734, 896
ClaI ATCGAT 1 cut(s) 2052
CseI GACGC 1 cut(s) 1046
Csp6I GTAC 7 cut(s) 461, 945, 1116, 1184, 1413, 1792, 1857
CviQI GTAC 7 cut(s) 461, 945, 1116, 1184, 1413, 1792, 1857
DraI TTTAAA 1 cut(s) 1908
Eam1104I CTCTTC 6 cut(s) 172, 286, 415, 677, 1442, 1959
EarI CTCTTC 6 cut(s) 172, 286, 415, 677, 1442, 1959
Eco130I CCWWGG 1 cut(s) 341
Eco147I AGGCCT 1 cut(s) 1820
Eco31I GGTCTC 1 cut(s) 393
Eco32I GATATC 2 cut(s) 780, 2185
Eco47I GGWCC 2 cut(s) 734, 896
Eco47III AGCGCT 1 cut(s) 838
Eco57I CTGAAG 2 cut(s) 267, 995
EcoNI CCTNNNNNAGG 1 cut(s) 1506
EcoRI GAATTC 2 cut(s) 179, 1729
EcoRII CCWGG 8 cut(s) 132, 504, 563, 672, 922, 1025, 1123, 1439
EcoRV GATATC 2 cut(s) 780, 2185
EcoT14I CCWWGG 1 cut(s) 341
EcoT22I ATGCAT 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 341
FalI AAGNNNNNCTT 2 cut(s) 606, 638
FaqI GGGAC 1 cut(s) 449
FauNDI CATATG 1 cut(s) 144
FblI GTMKAC 2 cut(s) 843, 1516
Fnu4HI GCNGC 6 cut(s) 28, 48, 450, 1136, 1725, 1739
FokI GGATG 9 cut(s) 130, 772, 1009, 1141, 1376, 1456, 1478, 1790, 2135
Fsp4HI GCNGC 6 cut(s) 28, 48, 450, 1136, 1725, 1739
FspBI CTAG 8 cut(s) 24, 81, 342, 530, 579, 804, 1631, 1718
GlaI GCGC 3 cut(s) 838, 1616, 2072
GluI GCNGC 6 cut(s) 28, 48, 450, 1136, 1725, 1739
GsaI CCCAGC 1 cut(s) 456
GsuI CTGGAG 5 cut(s) 488, 616, 695, 945, 1162
HaeII RGCGCY 2 cut(s) 840, 2074
HaeIII GGCC 3 cut(s) 1512, 1658, 1820
HapII CCGG 3 cut(s) 692, 789, 1659
HgaI GACGC 1 cut(s) 1046
HhaI GCGC 3 cut(s) 839, 1617, 2073
Hin6I GCGC 3 cut(s) 837, 1615, 2071
HinP1I GCGC 3 cut(s) 837, 1615, 2071
HincII GTYRAC 1 cut(s) 423
HindII GTYRAC 1 cut(s) 423
HpaII CCGG 3 cut(s) 692, 789, 1659
HphI GGTGA 5 cut(s) 533, 681, 832, 1182, 1949
Hpy166II GTNNAC 7 cut(s) 60, 423, 541, 844, 947, 1517, 1835
Hpy8I GTNNAC 7 cut(s) 60, 423, 541, 844, 947, 1517, 1835
HpyAV CCTTC 6 cut(s) 970, 1206, 1570, 1970, 2054, 2172
HpyCH4III ACNGT 6 cut(s) 57, 427, 990, 1490, 1832, 2048
HpyCH4IV ACGT 2 cut(s) 1607, 1859
HpyF10VI GCNNNNNNNGC 1 cut(s) 1682
HpySE526I ACGT 2 cut(s) 1607, 1859
HspAI GCGC 3 cut(s) 837, 1615, 2071
Kpn2I TCCGGA 1 cut(s) 788
LguI GCTCTTC 2 cut(s) 172, 1959
LmnI GCTCC 3 cut(s) 125, 566, 1452
Lsp1109I GCAGC 6 cut(s) 39, 59, 436, 1147, 1711, 1750
LweI GCATC 8 cut(s) 59, 108, 671, 1687, 1711, 1744, 2000, 2113
MaeI CTAG 8 cut(s) 24, 81, 342, 530, 579, 804, 1631, 1718
MaeII ACGT 2 cut(s) 1607, 1859
MaeIII GTNAC 8 cut(s) 427, 756, 825, 990, 1303, 1351, 1745, 2139
MfeI CAATTG 1 cut(s) 773
MflI RGATCY 2 cut(s) 1678, 2020
MhlI GDGCHC 2 cut(s) 62, 101
MlyI GAGTC 3 cut(s) 764, 1153, 1754
Mph1103I ATGCAT 1 cut(s) 264
MroI TCCGGA 1 cut(s) 788
MroXI GAANNNNTTC 1 cut(s) 2177
MslI CAYNNNNRTG 3 cut(s) 55, 65, 555
MspA1I CMGCKG 1 cut(s) 1595
MspI CCGG 3 cut(s) 692, 789, 1659
MspR9I CCNGG 9 cut(s) 134, 506, 565, 674, 924, 1027, 1125, 1441, 1660
MunI CAATTG 1 cut(s) 773
Mva1269I GAATGC 1 cut(s) 501
MvaI CCWGG 8 cut(s) 134, 506, 565, 674, 924, 1027, 1125, 1441
MwoI GCNNNNNNNGC 1 cut(s) 1682
NciI CCSGG 1 cut(s) 1660
NdeI CATATG 1 cut(s) 144
NlaIV GGNNCC 3 cut(s) 562, 1454, 1600
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 7 cut(s) 427, 756, 825, 990, 1303, 1351, 1745
NsiI ATGCAT 1 cut(s) 264
NspI RCATGY 5 cut(s) 165, 262, 1591, 1676, 2147
PaeI GCATGC 1 cut(s) 262
PaqCI CACCTGC 1 cut(s) 139
PceI AGGCCT 1 cut(s) 1820
PciI ACATGT 1 cut(s) 2143
PciSI GCTCTTC 2 cut(s) 172, 1959
PctI GAATGC 1 cut(s) 501
PdmI GAANNNNTTC 1 cut(s) 2177
PfeI GAWTC 7 cut(s) 205, 374, 524, 769, 929, 1238, 1469
PflMI CCANNNNNTGG 1 cut(s) 2030
PfoI TCCNGGA 2 cut(s) 504, 1025
PkrI GCNGC 6 cut(s) 29, 49, 451, 1137, 1726, 1740
PleI GAGTC 3 cut(s) 763, 1152, 1754
PpsI GAGTC 3 cut(s) 763, 1152, 1754
PscI ACATGT 1 cut(s) 2143
PshBI ATTAAT 1 cut(s) 384
Psp6I CCWGG 8 cut(s) 132, 504, 563, 672, 922, 1025, 1123, 1439
PspFI CCCAGC 1 cut(s) 452
PspGI CCWGG 8 cut(s) 132, 504, 563, 672, 922, 1025, 1123, 1439
PspN4I GGNNCC 3 cut(s) 562, 1454, 1600
PspPI GGNCC 2 cut(s) 734, 896
PsrI GAACNNNNNNTAC 2 cut(s) 565, 597
PstI CTGCAG 1 cut(s) 1086
PstNI CAGNNNCTG 1 cut(s) 1682
PsuI RGATCY 2 cut(s) 1678, 2020
PvuII CAGCTG 1 cut(s) 1595
RsaI GTAC 7 cut(s) 462, 946, 1117, 1185, 1414, 1793, 1858
RsaNI GTAC 7 cut(s) 461, 945, 1116, 1184, 1413, 1792, 1857
RseI CAYNNNNRTG 3 cut(s) 55, 65, 555
SapI GCTCTTC 2 cut(s) 172, 1959
SatI GCNGC 6 cut(s) 28, 48, 450, 1136, 1725, 1739
Sau96I GGNCC 2 cut(s) 734, 896
ScaI AGTACT 2 cut(s) 462, 1117
SchI GAGTC 3 cut(s) 764, 1153, 1754
ScrFI CCNGG 9 cut(s) 134, 506, 565, 674, 924, 1027, 1125, 1441, 1660
SduI GDGCHC 2 cut(s) 62, 101
SfaNI GCATC 8 cut(s) 59, 108, 671, 1687, 1711, 1744, 2000, 2113
SfcI CTRYAG 2 cut(s) 840, 1082
SinI GGWCC 2 cut(s) 734, 896
SmiMI CAYNNNNRTG 3 cut(s) 55, 65, 555
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
SphI GCATGC 1 cut(s) 262
SseBI AGGCCT 1 cut(s) 1820
SsiI CCGC 2 cut(s) 472, 1757
SspI AATATT 2 cut(s) 325, 369
SspMI CTAG 8 cut(s) 24, 81, 342, 530, 579, 804, 1631, 1718
StuI AGGCCT 1 cut(s) 1820
StyD4I CCNGG 9 cut(s) 132, 504, 563, 672, 922, 1025, 1123, 1439, 1658
StyI CCWWGG 1 cut(s) 341
TaaI ACNGT 6 cut(s) 57, 427, 990, 1490, 1832, 2048
TaiI ACGT 2 cut(s) 1610, 1862
TaqI TCGA 6 cut(s) 1255, 1712, 1994, 2052, 2058, 2181
TaqII GACCGA 1 cut(s) 1388
TatI WGTACW 3 cut(s) 460, 1115, 1412
TfiI GAWTC 7 cut(s) 205, 374, 524, 769, 929, 1238, 1469
TseFI GTSAC 7 cut(s) 427, 756, 825, 990, 1303, 1351, 1745
TseI GCWGC 6 cut(s) 27, 47, 449, 1135, 1724, 1738
Tsp45I GTSAC 7 cut(s) 427, 756, 825, 990, 1303, 1351, 1745
TspDTI ATGAA 4 cut(s) 539, 1218, 1380, 1460
TspGWI ACGGA 3 cut(s) 138, 215, 2135
Van91I CCANNNNNTGG 1 cut(s) 2030
VneI GTGCAC 1 cut(s) 58
VpaK11BI GGWCC 2 cut(s) 734, 896
VspI ATTAAT 1 cut(s) 384
XagI CCTNNNNNAGG 1 cut(s) 1506
XapI RAATTY 3 cut(s) 179, 1315, 1729
XbaI TCTAGA 2 cut(s) 529, 803
XceI RCATGY 5 cut(s) 165, 262, 1591, 1676, 2147
XmaJI CCTAGG 1 cut(s) 341
XmiI GTMKAC 2 cut(s) 843, 1516
XmnI GAANNNNTTC 1 cut(s) 2177
XspI CTAG 8 cut(s) 24, 81, 342, 530, 579, 804, 1631, 1718
ZrmI AGTACT 2 cut(s) 462, 1117
Zsp2I ATGCAT 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.