MD17G1010000.v1.1

DUF4210

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001019
Physical Location & Seq
Reverse (-)
618233 .. 622918
4686 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1010000.v1.1.491

Sequence Viewer

Length: 2295 bp
ATGGGATTGCCTCAAGTGCCCTCAACTGTAACTTCTGAGGAAGTGCTAGCTCCTTCTCCGGGCTCCTTTTTACAAAGCCCGCCTCGATTTTCTAGTATGAGCGCGTGTGATATGGATGGGATGCTTGGTGGCGGTGCAAGCCAAGTGGGAGGGAATCCATTATGCTCCTCTTTAGGAGATTACCAGACTAGAACCTCCCTAGAATTTTCAAAGCTCCCAGATGATTCACTTAGATTTAGAGGGACCGTGGACGTCACTAACAATGTTCATGGTTCTTCAATAGTTTCTGTTGATAAAGCTGATCAGATCACTGCAAGAAATGGACGGAATATTCAGACCCCTGCTTCTAGGATTGTAGGTTTTGAATCGACCGGAACAAGTTCTCTAAATGATGATTCGAGGGGTGTTTCATGTGGTCCTGTTCACTCTTCTTCTGCAGTTAATGTCACAACTAATGAAACTGTGACCAGTGGGTCACTAGTCAGGAAGCGATTGTTGTCCCCATTGAATACTATGCTTTTTCCACACCAGTTTAAAGGTGATCATTTGGACATTGGTTGCAGAACTTCTGGAACAAATTATCCTGCTATGGGTGATAATTTTAGTACACCTATTGCACATGATCATAAGAAAGCAAATGTTGGGATCAAAGGTAGTTTTACCAGGCCAACTTGTTCTCTGTCTAGTTACTTGGAGCAGAAGAGCATGCCATTGCTACATGATGATAGTATTATGGAAACCAGTTTTTTCACAGATGGCCCTTTGCTGGAAAACAAGGATCTTGTACCTCAAAATAGTTATCGATCTTCACCTAGACATGATGAGTTGACAAAATGGAGTAATGCAAAAATCCAAAGTGGGGAAATATCAATATCTCCGAAGGAAGTGATCTCACCTTTCCATTCTTTGTCACCTCTTGGCCCCAGGTTGTCTGAAAGATTGAAAATTGCAGGGGGCTGCAGGACTACCGACAAAGAGTTGGAGGATTGTCATTCAACCTTAAGGAATGTAAAACAAACACTTTACAAGTCTGATGCAGACATTCCCTTTTCCCCTGAAGTAGGGGAGTTTAGGATTGCTAGTAAACTATTTGAAGATGTTGATATTTTTCACAGGGAATTCCTTCCATCTTCTCTAGAGAACAGTACTGGCGTAAGTTATTCTTTGTCCCACGCCCACGGGTCAGCTCCCAACTCCCATTGCATGAGGTTTGTTAGAAGTTTGAGTGGCCTTTCTGTTCAGAGATCCTTGGTTGGTTCATTCGAAGAGTCTCTACTGTCTGGTCGGTTCATATCAGGCAAACCCAGTCAGAGAATAGATGGTTTTCTGGCTGTGCTGAGCATCACTGGGGGAAACTTTTCTCCGCAATCACAGAAGCTTCCTTTTTCAGTAACTAGTGTAGAAGGTGATTGCTATTTATTGTATTGCGCATCCATACATCTTTCGGGAAATTCTGCATTGAATAGGAGTGGAGGACAAAAGATTAAACAAGGCCTTAGCAATGATGGTTCACAGATTGTCACAAGCCGCCTGCGTATTCCTGTGAAGGGCCGTATACAACTGGTTCTAAGCAATCCTGAGAAGACACCTATTCATACGTTTCTTTGCAACTATGATTTAAGTGACATGCCAGCTGGTAGCAAAACTTTCTTACGCCAGAAGGTCACCTTAGCATGCAGCCCAAATTCTCCCCTGTCAAGACAAGGGAAGACTGATTTGAACTCAAAAGTGATAGAGGCGAAGGAAGTAGAGAATACAGCCGGAGTACACATTGTACATACAACAAGATCTGTAGATCAGAAAACTGAAATTAGAAGTGAAGGCTCTAATTTTGTGGATTCCATAGATGAGGGGGATATGTCCAAGGTATCCCCTAAAACGGGAAGGGTGTCCATTCCTTCGTTCCTAGTTGAGAAGAGCTTCAACAATGAGGAGTACCGAAGCAGCAACGGAAAGGAGCGCAAGTGTGTTGATGCATGCTATGGAACCGACAGGAAACATGTACATGGCTGTTCAAGGGTTAACCAAAGCAGTAATGGTGCTCTTCGCTATGCTCTTCATCTCCGTTTCATATGCCCTTTTCCTAAAAAAAGTTCTCGGTCAGTCCAGAGGTGCAAATCAGATCCCCTATCAACACCACAAAGAACAGGCTTAGACATGGATGGGGAGCGCAGGTTCTACCTGTACAATGACTTGAGGGTGGTGTTCCCTCAACGCCACTCAGACGCTGACGAGGGAAAGTTGAATGTGGAATACCATTTCCCGGATGATCCAAAGTACTTCGACATCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromosome_seg DUF4210

Protein Analysis

765

Amino Acids

83.63

Weight (kDa)

8.33

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4210 PF13915 417 - 463 1e-05 Domain of unknown function (DUF4210)
Chromosome_seg PF13889 681 - 763 4.9e-24 Chromosome segregation during meiosis
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 472
AatII GACGTC 1 cut(s) 255
Acc16I TGCGCA 1 cut(s) 1429
Acc36I ACCTGC 1 cut(s) 2163
AccI GTMKAC 1 cut(s) 1555
AccII CGCG 1 cut(s) 104
AciI CCGC 4 cut(s) 80, 132, 1364, 1528
AclWI GGATC 5 cut(s) 653, 786, 1239, 2117, 2264
AcsI RAATTY 4 cut(s) 203, 1118, 1450, 1684
AcuI CTGAAG 1 cut(s) 1077
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 9 cut(s) 607, 786, 1147, 1767, 1776, 1937, 2004, 2186, 2279
AfiI CCNNNNNNNGG 9 cut(s) 59, 590, 766, 859, 1061, 1547, 1879, 1880, 2263
AflII CTTAAG 1 cut(s) 1000
AflIII ACRYGT 1 cut(s) 1999
AhlI ACTAGT 2 cut(s) 478, 1394
AjnI CCWGG 2 cut(s) 662, 923
AloI GAACNNNNNNTCC 4 cut(s) 565, 597, 2159, 2191
AluBI AGCT 7 cut(s) 50, 214, 299, 1187, 1378, 1634, 1920
AluI AGCT 7 cut(s) 50, 214, 299, 1187, 1378, 1634, 1920
Alw21I GWGCWC 1 cut(s) 2044
Alw26I GTCTC 1 cut(s) 1275
AlwI GGATC 5 cut(s) 653, 786, 1239, 2117, 2264
AlwNI CAGNNNCTG 1 cut(s) 2228
AoxI GGCC 6 cut(s) 665, 757, 919, 1228, 1492, 1549
ApeKI GCWGC 3 cut(s) 957, 1677, 1944
ApoI RAATTY 4 cut(s) 203, 1118, 1450, 1684
Asp700I GAANNNNTTC 4 cut(s) 379, 1122, 1357, 1919
AspLEI GCGC 4 cut(s) 104, 1430, 1962, 2172
AspS9I GGNCC 5 cut(s) 243, 416, 758, 920, 1549
AsuC2I CCSGG 2 cut(s) 60, 2264
AsuHPI GGTGA 7 cut(s) 551, 605, 801, 885, 903, 1418, 1657
AsuII TTCGAA 1 cut(s) 1263
AsuNHI GCTAGC 1 cut(s) 46
AvaII GGWCC 2 cut(s) 243, 416
BaeGI GKGCMC 1 cut(s) 21
BanII GRGCYC 1 cut(s) 65
BarI GAAGNNNNNNTAC 2 cut(s) 1257, 1289
BbsI GAAGAC 2 cut(s) 1589, 1715
Bbv12I GWGCWC 1 cut(s) 2044
BbvI GCAGC 3 cut(s) 944, 1689, 1956
BccI CCATC 6 cut(s) 110, 749, 1135, 1313, 1499, 2156
BceAI ACGGC 1 cut(s) 1536
BciT130I CCWGG 2 cut(s) 664, 925
BciVI GTATCC 1 cut(s) 1879
BclI TGATCA 3 cut(s) 301, 541, 622
BcnI CCSGG 2 cut(s) 60, 2264
BcoDI GTCTC 1 cut(s) 1275
BcuI ACTAGT 2 cut(s) 478, 1394
BfmI CTRYAG 3 cut(s) 435, 958, 1791
BfrI CTTAAG 1 cut(s) 1000
BfuAI ACCTGC 1 cut(s) 2163
BfuI GTATCC 1 cut(s) 1879
BglII AGATCT 1 cut(s) 1787
BisI GCNGC 4 cut(s) 958, 1528, 1678, 1945
BlpI GCTNAGC 1 cut(s) 1337
BlsI GCNGC 4 cut(s) 959, 1529, 1679, 1946
BmcAI AGTACT 2 cut(s) 1147, 2279
Bme1390I CCNGG 4 cut(s) 60, 664, 925, 2264
Bme18I GGWCC 2 cut(s) 243, 416
BmgT120I GGNCC 5 cut(s) 243, 416, 758, 920, 1549
BmiI GGNNCC 4 cut(s) 64, 244, 922, 1987
BmrFI CCNGG 4 cut(s) 60, 664, 925, 2264
BmrI ACTGGG 2 cut(s) 1299, 1356
BmsI GCATC 5 cut(s) 111, 1024, 1350, 1439, 1963
BmtI GCTAGC 1 cut(s) 50
BmuI ACTGGG 2 cut(s) 1299, 1356
BpiI GAAGAC 2 cut(s) 1589, 1715
Bpu10I CCTNAGC 2 cut(s) 1496, 1669
Bpu1102I GCTNAGC 1 cut(s) 1337
Bpu14I TTCGAA 1 cut(s) 1263
BpuEI CTTGAG 1 cut(s) 2215
BpuMI CCSGG 2 cut(s) 60, 2264
Bsa29I ATCGAT 1 cut(s) 802
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 5 cut(s) 246, 923, 1177, 1248, 1863
BsaWI WCCGGW 1 cut(s) 371
BsaXI ACNNNNNCTCC 4 cut(s) 1949, 1979, 2159, 2189
Bsc4I CCNNNNNNNGG 9 cut(s) 59, 590, 766, 859, 1061, 1547, 1879, 1880, 2263
Bse1I ACTGG 7 cut(s) 468, 529, 741, 1153, 1305, 1351, 1566
Bse3DI GCAATG 3 cut(s) 710, 1198, 1507
BseBI CCWGG 2 cut(s) 664, 925
BseCI ATCGAT 1 cut(s) 802
BseDI CCNNGG 5 cut(s) 246, 923, 1177, 1248, 1863
BseGI GGATG 5 cut(s) 121, 126, 1430, 2167, 2272
BseLI CCNNNNNNNGG 9 cut(s) 59, 590, 766, 859, 1061, 1547, 1879, 1880, 2263
BseMI GCAATG 3 cut(s) 710, 1198, 1507
BseMII CTCAG 4 cut(s) 27, 1328, 1569, 2235
BseNI ACTGG 7 cut(s) 468, 529, 741, 1153, 1305, 1351, 1566
BseRI GAGGAG 2 cut(s) 157, 1946
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 3 cut(s) 944, 1689, 1956
Bsh1236I CGCG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 6 cut(s) 667, 759, 921, 1230, 1494, 1551
BshVI ATCGAT 1 cut(s) 802
BsiEI CGRYCG 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 2044
BsiSI CCGG 4 cut(s) 59, 372, 1761, 2264
BslFI GGGAC 3 cut(s) 256, 484, 1153
BslI CCNNNNNNNGG 9 cut(s) 59, 590, 766, 859, 1061, 1547, 1879, 1880, 2263
BsmAI GTCTC 1 cut(s) 1275
BsmFI GGGAC 3 cut(s) 256, 484, 1153
BsnI GGCC 6 cut(s) 667, 759, 921, 1230, 1494, 1551
Bsp119I TTCGAA 1 cut(s) 1263
Bsp1286I GDGCHC 3 cut(s) 21, 65, 2044
Bsp1407I TGTACA 3 cut(s) 1774, 2002, 2184
Bsp1720I GCTNAGC 1 cut(s) 1337
BspACI CCGC 4 cut(s) 80, 132, 1364, 1528
BspANI GGCC 6 cut(s) 667, 759, 921, 1230, 1494, 1551
BspCNI CTCAG 4 cut(s) 28, 1329, 1570, 2234
BspDI ATCGAT 1 cut(s) 802
BspFNI CGCG 1 cut(s) 104
BspLI GGNNCC 4 cut(s) 64, 244, 922, 1987
BspMAI CTGCAG 2 cut(s) 439, 962
BspMI ACCTGC 1 cut(s) 2163
BspOI GCTAGC 1 cut(s) 50
BspPI GGATC 5 cut(s) 653, 786, 1239, 2117, 2264
BspQI GCTCTTC 4 cut(s) 695, 1910, 2049, 2061
BspT104I TTCGAA 1 cut(s) 1263
BspTI CTTAAG 1 cut(s) 1000
BsrDI GCAATG 3 cut(s) 710, 1198, 1507
BsrGI TGTACA 3 cut(s) 1774, 2002, 2184
BsrI ACTGG 7 cut(s) 468, 529, 741, 1153, 1305, 1351, 1566
BssECI CCNNGG 5 cut(s) 246, 923, 1177, 1248, 1863
BssNAI GTATAC 1 cut(s) 1556
BssNI GRCGYC 1 cut(s) 252
BssT1I CCWWGG 2 cut(s) 1248, 1863
Bst1107I GTATAC 1 cut(s) 1556
Bst2UI CCWGG 2 cut(s) 664, 925
Bst4CI ACNGT 5 cut(s) 28, 247, 463, 1145, 1278
Bst6I CTCTTC 6 cut(s) 433, 695, 1260, 1910, 2049, 2061
BstACI GRCGYC 1 cut(s) 252
BstAFI CTTAAG 1 cut(s) 1000
BstAUI TGTACA 3 cut(s) 1774, 2002, 2184
BstBI TTCGAA 1 cut(s) 1263
BstC8I GCNNGC 8 cut(s) 48, 80, 139, 707, 1532, 1632, 1675, 1978
BstDEI CTNAG 9 cut(s) 36, 230, 1337, 1496, 1568, 1578, 1669, 2152, 2221
BstDSI CCRYGG 2 cut(s) 246, 1177
BstEII GGTNACC 1 cut(s) 1663
BstENI CCTNNNNNAGG 1 cut(s) 1059
BstF5I GGATG 5 cut(s) 121, 126, 1430, 2167, 2272
BstFNI CGCG 1 cut(s) 104
BstHHI GCGC 4 cut(s) 104, 1430, 1962, 2172
BstMAI GTCTC 1 cut(s) 1275
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 2 cut(s) 16, 138
BstNI CCWGG 2 cut(s) 664, 925
BstNSI RCATGY 5 cut(s) 709, 1630, 1677, 1980, 2003
BstPI GGTNACC 1 cut(s) 1663
BstSCI CCNGG 4 cut(s) 58, 662, 923, 2262
BstSFI CTRYAG 3 cut(s) 435, 958, 1791
BstSLI GKGCMC 1 cut(s) 21
BstUI CGCG 1 cut(s) 104
BstV1I GCAGC 3 cut(s) 944, 1689, 1956
BstV2I GAAGAC 2 cut(s) 1589, 1715
BstX2I RGATCY 4 cut(s) 778, 1244, 1787, 2122
BstYI RGATCY 4 cut(s) 778, 1244, 1787, 2122
BstZ17I GTATAC 1 cut(s) 1556
Bsu15I ATCGAT 1 cut(s) 802
BsuI GTATCC 1 cut(s) 1879
BsuRI GGCC 6 cut(s) 667, 759, 921, 1230, 1494, 1551
BsuTUI ATCGAT 1 cut(s) 802
BtgI CCRYGG 2 cut(s) 246, 1177
BtsCI GGATG 5 cut(s) 121, 126, 1430, 2167, 2272
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 3 cut(s) 309, 475, 1344
BveI ACCTGC 1 cut(s) 2163
Cac8I GCNNGC 8 cut(s) 48, 80, 139, 707, 1532, 1632, 1675, 1978
CaiI CAGNNNCTG 1 cut(s) 2228
CfoI GCGC 4 cut(s) 104, 1430, 1962, 2172
Cfr13I GGNCC 5 cut(s) 243, 416, 758, 920, 1549
ClaI ATCGAT 1 cut(s) 802
CseI GACGC 1 cut(s) 2234
Csp6I GTAC 9 cut(s) 606, 785, 1146, 1766, 1775, 1936, 2003, 2185, 2278
CspCI CAANNNNNGTGG 2 cut(s) 126, 161
CviQI GTAC 9 cut(s) 606, 785, 1146, 1766, 1775, 1936, 2003, 2185, 2278
DdeI CTNAG 9 cut(s) 36, 230, 1337, 1496, 1568, 1578, 1669, 2152, 2221
DraI TTTAAA 1 cut(s) 535
DrdI GACNNNNNNGTC 1 cut(s) 472
DseDI GACNNNNNNGTC 1 cut(s) 472
Eam1104I CTCTTC 6 cut(s) 433, 695, 1260, 1910, 2049, 2061
EarI CTCTTC 6 cut(s) 433, 695, 1260, 1910, 2049, 2061
Eco130I CCWWGG 2 cut(s) 1248, 1863
Eco147I AGGCCT 1 cut(s) 1494
Eco24I GRGCYC 1 cut(s) 65
Eco47I GGWCC 2 cut(s) 243, 416
Eco57I CTGAAG 1 cut(s) 1077
Eco91I GGTNACC 1 cut(s) 1663
EcoNI CCTNNNNNAGG 1 cut(s) 1059
EcoO65I GGTNACC 1 cut(s) 1663
EcoRI GAATTC 1 cut(s) 1118
EcoRII CCWGG 2 cut(s) 662, 923
EcoT14I CCWWGG 2 cut(s) 1248, 1863
EcoT22I ATGCAT 1 cut(s) 1978
EcoT38I GRGCYC 1 cut(s) 65
ErhI CCWWGG 2 cut(s) 1248, 1863
FalI AAGNNNNNCTT 4 cut(s) 1147, 1179, 1652, 1684
FaqI GGGAC 3 cut(s) 256, 484, 1153
FauI CCCGC 1 cut(s) 87
FauNDI CATATG 1 cut(s) 2072
FbaI TGATCA 3 cut(s) 301, 541, 622
FblI GTMKAC 1 cut(s) 1555
Fnu4HI GCNGC 4 cut(s) 958, 1528, 1678, 1945
FokI GGATG 5 cut(s) 128, 133, 1417, 2174, 2279
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 4 cut(s) 958, 1528, 1678, 1945
FspI TGCGCA 1 cut(s) 1429
GlaI GCGC 4 cut(s) 103, 1429, 1961, 2171
GluI GCNGC 4 cut(s) 958, 1528, 1678, 1945
HaeIII GGCC 6 cut(s) 667, 759, 921, 1230, 1494, 1551
HapII CCGG 4 cut(s) 59, 372, 1761, 2264
HgaI GACGC 1 cut(s) 2234
HhaI GCGC 4 cut(s) 104, 1430, 1962, 2172
Hin1I GRCGYC 1 cut(s) 252
Hin6I GCGC 4 cut(s) 102, 1428, 1960, 2170
HinP1I GCGC 4 cut(s) 102, 1428, 1960, 2170
HincII GTYRAC 2 cut(s) 828, 2023
HindII GTYRAC 2 cut(s) 828, 2023
HindIII AAGCTT 1 cut(s) 1376
HinfI GANTC 6 cut(s) 154, 224, 365, 395, 1268, 1838
HpaI GTTAAC 1 cut(s) 2023
HpaII CCGG 4 cut(s) 59, 372, 1761, 2264
HphI GGTGA 7 cut(s) 551, 605, 801, 885, 903, 1418, 1657
Hpy166II GTNNAC 9 cut(s) 250, 424, 608, 828, 1085, 1511, 1556, 1768, 2023
Hpy188III TCNNGA 7 cut(s) 484, 570, 1136, 1446, 1577, 1698, 2107
Hpy8I GTNNAC 9 cut(s) 250, 424, 608, 828, 1085, 1511, 1556, 1768, 2023
HpyCH4III ACNGT 5 cut(s) 28, 247, 463, 1145, 1278
HpyCH4IV ACGT 2 cut(s) 252, 1598
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 138
HpyF3I CTNAG 9 cut(s) 36, 230, 1337, 1496, 1568, 1578, 1669, 2152, 2221
HpySE526I ACGT 2 cut(s) 252, 1598
Hsp92I GRCGYC 1 cut(s) 252
HspAI GCGC 4 cut(s) 102, 1428, 1960, 2170
Ksp22I TGATCA 3 cut(s) 301, 541, 622
KspAI GTTAAC 1 cut(s) 2023
LguI GCTCTTC 4 cut(s) 695, 1910, 2049, 2061
LmnI GCTCC 8 cut(s) 55, 68, 170, 219, 694, 1192, 1957, 2167
Lsp1109I GCAGC 3 cut(s) 944, 1689, 1956
LweI GCATC 5 cut(s) 111, 1024, 1350, 1439, 1963
MaeII ACGT 2 cut(s) 252, 1598
MflI RGATCY 4 cut(s) 778, 1244, 1787, 2122
MhlI GDGCHC 3 cut(s) 21, 65, 2044
MluCI AATT 9 cut(s) 203, 577, 598, 945, 1118, 1450, 1684, 1809, 1828
MlyI GAGTC 1 cut(s) 1277
MmeI TCCRAC 1 cut(s) 960
Mph1103I ATGCAT 1 cut(s) 1978
MroXI GAANNNNTTC 4 cut(s) 379, 1122, 1357, 1919
MseI TTAA 6 cut(s) 441, 534, 1001, 1485, 1619, 2022
MslI CAYNNNNRTG 1 cut(s) 2004
MspA1I CMGCKG 1 cut(s) 1634
MspCI CTTAAG 1 cut(s) 1000
MspI CCGG 4 cut(s) 59, 372, 1761, 2264
MspR9I CCNGG 4 cut(s) 60, 664, 925, 2264
MvaI CCWGG 2 cut(s) 664, 925
MvnI CGCG 1 cut(s) 104
MwoI GCNNNNNNNGC 2 cut(s) 16, 138
NciI CCSGG 2 cut(s) 60, 2264
NdeI CATATG 1 cut(s) 2072
NheI GCTAGC 1 cut(s) 46
NlaIV GGNNCC 4 cut(s) 64, 244, 922, 1987
NmuCI GTSAC 8 cut(s) 253, 445, 463, 474, 909, 1519, 1622, 1663
NsbI TGCGCA 1 cut(s) 1429
NsiI ATGCAT 1 cut(s) 1978
NspI RCATGY 5 cut(s) 709, 1630, 1677, 1980, 2003
NspV TTCGAA 1 cut(s) 1263
PaeI GCATGC 3 cut(s) 709, 1677, 1980
PceI AGGCCT 1 cut(s) 1494
PciI ACATGT 1 cut(s) 1999
PciSI GCTCTTC 4 cut(s) 695, 1910, 2049, 2061
PdmI GAANNNNTTC 4 cut(s) 379, 1122, 1357, 1919
PfeI GAWTC 5 cut(s) 154, 224, 365, 395, 1838
PfoI TCCNGGA 1 cut(s) 2262
PkrI GCNGC 4 cut(s) 959, 1529, 1679, 1946
PleI GAGTC 1 cut(s) 1276
PpsI GAGTC 1 cut(s) 1276
PscI ACATGT 1 cut(s) 1999
Psp6I CCWGG 2 cut(s) 662, 923
PspEI GGTNACC 1 cut(s) 1663
PspGI CCWGG 2 cut(s) 662, 923
PspN4I GGNNCC 4 cut(s) 64, 244, 922, 1987
PspPI GGNCC 5 cut(s) 243, 416, 758, 920, 1549
PstI CTGCAG 2 cut(s) 439, 962
PstNI CAGNNNCTG 1 cut(s) 2228
PsuI RGATCY 4 cut(s) 778, 1244, 1787, 2122
PvuII CAGCTG 1 cut(s) 1634
RsaI GTAC 9 cut(s) 607, 786, 1147, 1767, 1776, 1937, 2004, 2186, 2279
RsaNI GTAC 9 cut(s) 606, 785, 1146, 1766, 1775, 1936, 2003, 2185, 2278
RseI CAYNNNNRTG 1 cut(s) 2004
SapI GCTCTTC 4 cut(s) 695, 1910, 2049, 2061
SaqAI TTAA 6 cut(s) 441, 534, 1001, 1485, 1619, 2022
SatI GCNGC 4 cut(s) 958, 1528, 1678, 1945
Sau96I GGNCC 5 cut(s) 243, 416, 758, 920, 1549
ScaI AGTACT 2 cut(s) 1147, 2279
SchI GAGTC 1 cut(s) 1277
ScrFI CCNGG 4 cut(s) 60, 664, 925, 2264
SduI GDGCHC 3 cut(s) 21, 65, 2044
SfaNI GCATC 5 cut(s) 111, 1024, 1350, 1439, 1963
SfcI CTRYAG 3 cut(s) 435, 958, 1791
SfuI TTCGAA 1 cut(s) 1263
SinI GGWCC 2 cut(s) 243, 416
SmiMI CAYNNNNRTG 1 cut(s) 2004
SmlI CTYRAG 3 cut(s) 12, 1000, 2194
SmoI CTYRAG 3 cut(s) 12, 1000, 2194
SpeI ACTAGT 2 cut(s) 478, 1394
SphI GCATGC 3 cut(s) 709, 1677, 1980
Sse9I AATT 9 cut(s) 203, 577, 598, 945, 1118, 1450, 1684, 1809, 1828
SseBI AGGCCT 1 cut(s) 1494
SsiI CCGC 4 cut(s) 80, 132, 1364, 1528
SspI AATATT 1 cut(s) 331
StuI AGGCCT 1 cut(s) 1494
StyD4I CCNGG 4 cut(s) 58, 662, 923, 2262
StyI CCWWGG 2 cut(s) 1248, 1863
TaaI ACNGT 5 cut(s) 28, 247, 463, 1145, 1278
TaiI ACGT 2 cut(s) 255, 1601
TaqI TCGA 6 cut(s) 85, 368, 398, 802, 1263, 2283
TaqII GACCGA 1 cut(s) 2088
TasI AATT 9 cut(s) 203, 577, 598, 945, 1118, 1450, 1684, 1809, 1828
TatI WGTACW 7 cut(s) 605, 1145, 1765, 1774, 2002, 2184, 2277
TauI GCSGC 1 cut(s) 1530
TfiI GAWTC 5 cut(s) 154, 224, 365, 395, 1838
Tru1I TTAA 6 cut(s) 441, 534, 1001, 1485, 1619, 2022
Tru9I TTAA 6 cut(s) 441, 534, 1001, 1485, 1619, 2022
TscAI CASTG 3 cut(s) 316, 475, 1351
TseFI GTSAC 8 cut(s) 253, 445, 463, 474, 909, 1519, 1622, 1663
TseI GCWGC 3 cut(s) 957, 1677, 1944
Tsp45I GTSAC 8 cut(s) 253, 445, 463, 474, 909, 1519, 1622, 1663
TspDTI ATGAA 8 cut(s) 257, 399, 471, 1248, 1279, 1583, 2048, 2059
TspGWI ACGGA 3 cut(s) 340, 1965, 2054
TspRI CASTG 3 cut(s) 316, 475, 1351
Vha464I CTTAAG 1 cut(s) 1000
VpaK11BI GGWCC 2 cut(s) 243, 416
XagI CCTNNNNNAGG 1 cut(s) 1059
XapI RAATTY 4 cut(s) 203, 1118, 1450, 1684
XbaI TCTAGA 1 cut(s) 1135
XceI RCATGY 5 cut(s) 709, 1630, 1677, 1980, 2003
XcmI CCANNNNNNNNNTGG 1 cut(s) 2033
XmiI GTMKAC 1 cut(s) 1555
XmnI GAANNNNTTC 4 cut(s) 379, 1122, 1357, 1919
ZraI GACGTC 1 cut(s) 253
ZrmI AGTACT 2 cut(s) 1147, 2279
Zsp2I ATGCAT 1 cut(s) 1978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.