Rorug02G0591900

DUF4210

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001019
Physical Location & Seq
Reverse (-)
71391660 .. 71399948
8289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0591900.1

Sequence Viewer

Length: 5412 bp
ATGTTAGCTAATCTTACAATACCCTCGGAGCATTATGATCCAATCCCAGTGGAAATGGGAGCCCGAATATTGAGAATTGCATGGAATAATCTGGAAGATCCTTTAAGTCAAACAGTTAAACAAGCTCATCTTATTTTTGATCTCTTCCTAGACATCCGTTCCACCCTTTGTTGGTCAGAAGGTAGTGAGAGAATAAGGTCATTCTTGCAAAATATTGCTTCTGATCTTCTTCGTTTGGGTCCTCGCTGCAAGGGGAGGTATGTTCCTTTAGCTTCACTGACCAAGCGATTGGGCGCAAAAACTATGTTAAATATGAGTCCTAACCTGATGTATGAGATTGTACATGCCTATGTTGATGATGATGTCTGCTGTGCTGTCACATCATTTTTGAAGTGTTTACTTGAGCACTTGCGTGACGAGTGTTGGAGCAGCGATGGTATTGAAGGTGGTTATGCACTTTACAGAGGGCATTGTTTGCCCCCATTATTGTATGGACTTTCTTCTGGGGTTTCAAGGCTCCGCTCTAATTTGAACACATATGCTCTTCCAATTTTACTTGAAGTGGATGAGGACAGCTTATTTTCCATGCTGGCATTTATTTCAGTTGGTCCGAGTAAGGGTGATGATCAACTGTTATATCCTGAGCTCTTTTGTGATAACATAGTATTGAGGGTTGAACAAAAGGTGGCAATCTTAGTTTCATTGCTCAAGGTATCTCGTTTGCTTGCTTTGATTGAAGGGGACATTGATTGGTGCAAAGATTCTGATCAGTATGCTCTTGTTTGTATCAAGGGAACAAAAGTTGAAGTTGTGGTCGAGTGGTTAGTGCTTGCGTTGACCCATGTTGATGAGTCATTACGTGTGGATGCTGCAGAGACTCTTTTCTTGAATCCCAAGACGGCTAGTTTGCCTTCCCATTTAGAACTCATGTTGTTCAGGGAAGCAGTCCCACTTAACATGAGATGTTGCTCTACAGGTTTTCAAATGAAGTGGAGTAGCTTGTTTAGAAAGTTTTTTTCTCGTGTGCGAACAGCCTTGGAGAGACAATTCAAGCAGGGAAGCTGGCAGCCGATTGAGGATAACAACAACAGTGGAAAACATCTTTCAAATGGAAGTGAGCACACTGAAGCTCACAGAGCAAGTGATCTTTTTCATTTCATGAGGTGGTTAAGTTCATTTCTATTTTTCTCATGCTATCCTTCTGCCCCTTATCAGAGAAAAATAATGGCCACACAGCTGATTCTGATAATGCTTAATGTTTGGTCCATTGTGCCTGCTACTGAAGAAAAATTTGGCTCTGTTTCTTTAGAAGGCTGTCTCTATCCTTATAATAAGGGAACGACTTCACCTGATACAACTTTGTTGTTAGTTGGATCAATAATTGATAGTTGGGACAGGCTGAGAGAGTGTTTCTTTCGTATACTGTTACACTTTCCAAATCCACTTCCGGGAATTTCAGACGAAGATATGGTCCAGAACGTGGTTTCATGGGCTAAGAAATTAGTTTGCAGTCCGCGTGTGAGAGAAAGTGATGCTGGAGCTCTGGCTTTAAGGCTAATTTTCAGGAAGTATGTCTTACAGCTAGGATGGACAGTTCAAGCTTCAGTTTGTGTAGCTTGCATTCAATCACAGTCTGGATTGGAAAACGGAGATTGTCAGACTTATAATTCTAGGCACCCTGTGATAGAATATGTTAGATCACTGATTGATTGGTTGGATGTTTCCATAGTGGAAGGGGAGAGGGATCTTTCAGAAGCCTGCAAAAGCAGCTTTGTCCATGGAGTATTACTTACACTACGATATACTTTTGAGGAACTAGACTTCAGCACTGATGGAGTACTGTCTAGTATTTCAGAGATGAGACATCTATTAGAGAAGCTCTTGGAGCTTGTGATGCGAATAACTTCTTTGGCACTTTGGGTGGTTTCTGCAGATGCTTTGCAGCTTCCTGAGGACATGGATGATATGGTTGATGATGAAACTTTCTTATCCGAGGTTCCAGAAGAGGTGGAGGCAAAAACATCTCTTTTGGAGCATGGAGACAATAACTCCACACTTCTGCAGGATAATAGGCGATCGGAACAAACTGTAATGGTTGGTTGCTGGCTGGCGATGAAAGAGGTGAGTCTTCTTTTGGGAACTATTGTAAGAAAGGTTCCTTTACCAAGTAGCCCTTCCTCAGAATCATTACATGCAGAAGCCACCTCTTGTGCTTCTGGAGTTTCAGATATGATGGTTTCGGATGCAATGCTTGATTTGAAACAACTTGAAACAATTGGGAATCACTTCTTGGAAGTCCTTCTTAAAATGAAGCATAATGGTGCCATTGATAAAACAAGGGCTGGATTTACAGCTCTTTGCAACCGTTTACTTTGCTCAAATGATCCTAGGCTTTGTAAGTTAACAGAGTCCTGGATGGAGCAACTTATGGAAAGAACTGTGGCCAAGGGTCAAGTAGTGGATGACTTGCTAAGGAGAAGTGCAGGTATTCCTGCTGCATTTACAGCTCTATTTCTCTCAGAGCCTGAAGGTGCACCTAAGAAACTTCTCCCACGGGCCTTGCGGTGGCTGATAGATGTTGCTAAAGAGTCTTTTATGGATCAATTTGAAACCAGCAGCTCCAATGGTGACATGCGTAAATTTTGTTCGACAAAGTCGGACAATAGTTTTGATTCTGCACTGCCATCAGAGAGAAATATAAGTGACAAAGTTTCAAAGATCCGAGATGAGGGTGTCGTTCCTACCGTACATGCATTCAATGTCCTTAGGGCTGCTTTCAATGACACCAACCTGGCCACTGATACATCAGGGTTTTCTGCTGAGGCTATGATTATTTCAATTCGCTCCTTTTCTTCTCCCTACTGGGAGGTTCGGAACAGTGCTTGTCAGGCATACACTGCTTTAGTACGTCGCATGATTGGATTCCTTAATGTCCAGAAACGGGAGTCGCAAAGGCGTGCACTAACTGGGGTTGAATTTTTTCATAGGTATCCCTCTTTGCATCCATTTTTATTCAAGGAACTGAAAGCTGCAACTGAGTTGCTTGGGGATGGGCCATCTGGACAGTCTGGGTGTAATCTGGAAGATGCTGTGCACCCAAGCTTGTGTCCTGTGTTGATTCTGTTATCCAGGCTTAAGCCCTCAACTATTGCAAGTGAGGCTGGAGATGACCTAGATCCTTCTTTGTTCATGCCGTTCATTAGGAGCTGCTCAACCCAAAGCAATCTCAGAGTCCGTGTCCTTGCATCTAGAGCTTTAACAGGCCTTGTATCTAATGAGAAATTGCCAACTGTTATTCTCAATATAGTATCAGAGTTGCCTAGCATAGACAACCAAGCTACAATGACTCCTGAGTCGTCCTTGCTACTTCACAAAACCAGAAGAAGTAATTGGATTCATGGAGTTCTGTTGCAGTTGAGTTCTCTCCTGGATACAAACTGTAGAAATCTGGCCGATGTCGTAAAGAAAGATCAGATTTTAGGTGACTTATTTGAAGCTCTTCTAAGTCATTCATGGATGGCAAAGCCCAGATGTTGCCCTTGTCCCATCCTCAATGCCTCTTTCTTAAAGCTGCTGGACCACATGCTCAGTATTGCGAGGACAAGCCATACCAGCAGACATTTTTATGCCCTCCGCAACCTACTGTTACAGTTATCTACAGAATGCCTAGATGTAGAAGCTTCTCATGGGTTCTCTTATTATGATCCGACAATAGCTCAACTCCGGCAACAAGCAGCAGCATCCTATTTCAGTTGCGTCTTTCAAGCAACTGAGGAAATGGCTGCAGAGGCTTTTCAGATGCCTCAGAGGTACTCTCATATTGATTCAACTAATCAGAAGATACCTGAAACAGAGAATGCTTTTATAGGACTCCAGGAAAGACTGGTCCGGTCTCTGTCAGATTCGGAATACGAAGTTCGACTTGCAACATTGAAGTGGCTACTGAAATTCCTAACATCAACAGAATCTGGTAGTGGGTTCCATAATTTTAGCAGTGAGGTTAGGATCATTCGCCACTGGATCAGAACTAAACTCCAGACCTCATTGGTTGATCTCTTAGATGTGGAGAAGTATTACAGATGCTCATATTACATTCTGAGAATTATATTCACTTGGAATGCGCTACAGTTCCAGAAGCCTAGGGATGAAAAATGCACTGAATCAGTTTATGTTGGTAGTATGGAATGTGATTCTGTGTTTCTGCTTTGGGATAAGTTGATTTCCTTGTACAATCTCACAAGACATGCAAAGACTCGAGAAACACTCGTATGCTGCATTGGAATCTGCATAAAGAGGTTTGCAGGTTTATTTACAACTTCTGTTCTTTCTGATGTAACGGACAACAATGAGTCTGATCAGTTAGAAAAACTGGGCGCACTTCATAGTAGAATTTCCTTTTTCACTGAATTAATTAAGAAACATAGTGCTTCATCTCAGCCGGTAAACATGCGTAAGGCAGCAGCAGAGTCTATCATAGCATCTGGTTTGCTAGAGCAAGCTGAGTTGATTGGTTCTACTGTGTTCAATAGCCAAACCCATTTGGAAAGTTCATCGTCTCATTTTGAAGGAAAAGAAGCGGTTAATATCTATGCTCATCAAATAGTAGATATATGGTTCACATGTATCAAGCTGCTGGAGGATGAAGATGACGAGATTAGGCAAAGGCTCGCCATGGGTATTCAAAGGTGTTTTTCTTGCCAAAGATCCGGAAGAAGCTCTCTTACTGGAGAAGTTCCAACTCAAGTGGAGAAAGTGATAGAGTCGAGTTTCGAGCATCTAACTTCCATCTTTGGTCACTGGATTGGGTATTTGGATTTTCTTTTACACAGTGTACTGAATGCAGCAAGCCATGAGGTGTCCAAAGGAGATCTTGTGAGACAGGTATTTGATAAGGAAATCGATAATCATCATGAGGAAAAGCTTTTGATCTGCCAGCTTTGCTGTTCTCAATTAGAGAAGCTTCCGATTACAAAATCTTGTGCTGTCGACATTTCAGACAAACAGCAGTTTAGGGATTATTTACATGATTGGAGACTCAGGTTTTCCCGCCAATTGATGTCGTTTGCCAAGGACCGCATGGCGAAACTAGGTGGAGCCGATTGGGTTGGTGGAGTGGGCAACCATAAGGATTCATTTCTCCCCCTGTATTCCAGTTTGCTTGCATTTCATGCCATCTCGAACTGCATGTTGAAAGGGAATACGGAAGATAATACGCATCTACTGTCTGATGTTGTTGAAGTCGGGAGAACTATCAGTCCATTTCTGAAGAACCCTCTGATCTCTAACCTGTATTTTTCAGTACTTAAATCTCATGAAGATGCTCTTGGTCCGACCAATGATGACTTGATCTCCAAACTGAGAGGAGAGGATGCAATATGGAAGGGGTTCAATCCCCATTTTCTTCTTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromosome_seg DUF4210

Protein Analysis

1803

Amino Acids

203.04

Weight (kDa)

5.83

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_Trm732 PF25150 160 - 468 1.9e-17 Trm732, TPR repeats
DUF2428 PF10350 623 - 956 7.5e-74 THADA/TRM732, DUF2428
TPR_Trm732_C PF25151 958 - 1141 3.2e-32 Trm732, C-terminal TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1329, 1665
AasI GACNNNNNNGTC 1 cut(s) 3352
Acc36I ACCTGC 2 cut(s) 2474, 4292
AccB1I GGYRCC 2 cut(s) 1674, 2321
AccB7I CCANNNNNTGG 1 cut(s) 1480
AccBSI CCGCTC 1 cut(s) 522
AccI GTMKAC 2 cut(s) 1420, 4986
AccII CGCG 1 cut(s) 1516
AccIII TCCGGA 1 cut(s) 4706
AciI CCGC 7 cut(s) 520, 1514, 2563, 3634, 4577, 5047, 5074
AcoI YGGCCR 4 cut(s) 1227, 2442, 2793, 3450
AcsI RAATTY 6 cut(s) 1289, 1452, 2639, 2975, 3947, 4389
AcuI CTGAAG 6 cut(s) 1146, 1302, 1587, 1807, 2547, 5285
AdeI CACNNNGTG 1 cut(s) 1681
AfaI GTAC 8 cut(s) 342, 1839, 2748, 2907, 3812, 4229, 4833, 5301
AfiI CCNNNNNNNGG 7 cut(s) 171, 617, 1448, 1480, 2565, 2728, 2941
AflII CTTAAG 1 cut(s) 3134
AflIII ACRYGT 2 cut(s) 859, 4619
AjnI CCWGG 5 cut(s) 2411, 2790, 3128, 3426, 3873
AleI CACNNNNGTG 1 cut(s) 411
AloI GAACNNNNNNTCC 4 cut(s) 1578, 1610, 5239, 5271
Alw21I GWGCWC 7 cut(s) 408, 648, 1122, 1543, 2536, 2962, 3096
Alw26I GTCTC 9 cut(s) 869, 1036, 1322, 1855, 2034, 3897, 4560, 4870, 5026
Alw44I GTGCAC 3 cut(s) 2532, 2958, 3092
AlwNI CAGNNNCTG 2 cut(s) 2525, 3968
Ama87I CYCGRG 1 cut(s) 4254
Aor13HI TCCGGA 1 cut(s) 4706
AoxI GGCC 7 cut(s) 1227, 2442, 2556, 2793, 3053, 3262, 3450
ApaLI GTGCAC 3 cut(s) 2532, 2958, 3092
ApoI RAATTY 6 cut(s) 1289, 1452, 2639, 2975, 3947, 4389
ArsI GACNNNNNNTTYG 6 cut(s) 889, 921, 1455, 1487, 2651, 2683
AseI ATTAAT 1 cut(s) 4409
Asp700I GAANNNNTTC 7 cut(s) 1342, 1903, 2285, 2298, 2979, 3498, 5384
AspA2I CCTAGG 2 cut(s) 2387, 4139
AspLEI GCGC 3 cut(s) 296, 4123, 4376
AsuC2I CCSGG 1 cut(s) 1449
AsuHPI GGTGA 5 cut(s) 632, 1338, 2134, 2639, 3494
AvaI CYCGRG 1 cut(s) 4254
AvaII GGWCC 8 cut(s) 239, 608, 1263, 1471, 3577, 3886, 5071, 5327
AvrII CCTAGG 2 cut(s) 2387, 4139
AxyI CCTNAGG 2 cut(s) 1950, 2765
BaeGI GKGCMC 3 cut(s) 2536, 2962, 3096
BalI TGGCCA 3 cut(s) 1229, 2444, 2795
BanI GGYRCC 2 cut(s) 1674, 2321
BanII GRGCYC 3 cut(s) 64, 648, 1543
BarI GAAGNNNNNNTAC 2 cut(s) 4705, 4737
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 1 cut(s) 2120
Bbv12I GWGCWC 7 cut(s) 408, 648, 1122, 1543, 2536, 2962, 3096
BbvCI CCTCAGC 1 cut(s) 2820
BceAI ACGGC 2 cut(s) 915, 3178
BciT130I CCWGG 5 cut(s) 2413, 2792, 3130, 3428, 3875
BciVI GTATCC 2 cut(s) 3000, 3424
BclI TGATCA 3 cut(s) 625, 766, 4354
BcnI CCSGG 1 cut(s) 1449
BcoDI GTCTC 9 cut(s) 869, 1036, 1322, 1855, 2034, 3897, 4560, 4870, 5026
BfmI CTRYAG 8 cut(s) 870, 972, 1929, 2060, 3439, 3657, 3783, 4124
BfrI CTTAAG 1 cut(s) 3134
BfuAI ACCTGC 2 cut(s) 2474, 4292
BfuI GTATCC 2 cut(s) 3000, 3424
BglII AGATCT 1 cut(s) 4867
BlnI CCTAGG 2 cut(s) 2387, 4139
BmcAI AGTACT 2 cut(s) 1839, 5301
Bme1390I CCNGG 6 cut(s) 1449, 2413, 2792, 3130, 3428, 3875
Bme18I GGWCC 8 cut(s) 239, 608, 1263, 1471, 3577, 3886, 5071, 5327
BmeT110I CYCGRG 1 cut(s) 4254
BmiI GGNNCC 9 cut(s) 61, 240, 518, 1676, 1998, 2157, 2323, 3980, 5095
BmrFI CCNGG 6 cut(s) 1449, 2413, 2792, 3130, 3428, 3875
BmrI ACTGGG 4 cut(s) 41, 2872, 2976, 4379
BmuI ACTGGG 4 cut(s) 41, 2872, 2976, 4379
BpiI GAAGAC 1 cut(s) 2120
BplI GAGNNNNNCTC 4 cut(s) 3799, 3831, 4248, 4280
BpmI CTGGAG 7 cut(s) 1557, 2238, 3183, 3857, 4019, 4655, 4746
Bpu10I CCTNAGC 3 cut(s) 642, 2471, 2820
BpuEI CTTGAG 3 cut(s) 422, 692, 4725
BpuMI CCSGG 1 cut(s) 1449
Bsa29I ATCGAT 1 cut(s) 4899
BsaAI YACGTR 1 cut(s) 860
BsaBI GATNNNNATC 2 cut(s) 765, 4905
BsaI GGTCTC 1 cut(s) 3897
BsaWI WCCGGW 2 cut(s) 3888, 4706
BsaXI ACNNNNNCTCC 8 cut(s) 2033, 2063, 3331, 3361, 4857, 4887, 5333, 5363
Bsc4I CCNNNNNNNGG 7 cut(s) 171, 617, 1448, 1480, 2565, 2728, 2941
Bse118I RCCGGY 1 cut(s) 4438
Bse1I ACTGG 9 cut(s) 47, 2867, 2971, 3888, 4022, 4374, 4729, 4802, 5151
Bse21I CCTNAGG 2 cut(s) 1950, 2765
Bse3DI GCAATG 2 cut(s) 701, 2253
Bse8I GATNNNNATC 2 cut(s) 765, 4905
BseAI TCCGGA 1 cut(s) 4706
BseBI CCWGG 5 cut(s) 2413, 2792, 3130, 3428, 3875
BseCI ATCGAT 1 cut(s) 4899
BseJI GATNNNNATC 2 cut(s) 765, 4905
BseLI CCNNNNNNNGG 7 cut(s) 171, 617, 1448, 1480, 2565, 2728, 2941
BseMI GCAATG 2 cut(s) 701, 2253
BseNI ACTGG 9 cut(s) 47, 2867, 2971, 3888, 4022, 4374, 4729, 4802, 5151
BseRI GAGGAG 1 cut(s) 5376
BseSI GKGCMC 3 cut(s) 2536, 2962, 3096
BsgI GTGCAG 2 cut(s) 2502, 2661
Bsh1236I CGCG 1 cut(s) 1516
Bsh1285I CGRYCG 1 cut(s) 2078
BshFI GGCC 7 cut(s) 1229, 2444, 2558, 2795, 3055, 3264, 3452
BshNI GGYRCC 2 cut(s) 1674, 2321
BshVI ATCGAT 1 cut(s) 4899
BsiEI CGRYCG 1 cut(s) 2078
BsiHKAI GWGCWC 7 cut(s) 408, 648, 1122, 1543, 2536, 2962, 3096
BsiHKCI CYCGRG 1 cut(s) 4254
BsiSI CCGG 5 cut(s) 1448, 3724, 3889, 4439, 4707
BslFI GGGAC 4 cut(s) 755, 932, 1406, 3528
BslI CCNNNNNNNGG 7 cut(s) 171, 617, 1448, 1480, 2565, 2728, 2941
BsmAI GTCTC 9 cut(s) 869, 1036, 1322, 1855, 2034, 3897, 4560, 4870, 5026
BsmBI CGTCTC 1 cut(s) 4560
BsmFI GGGAC 4 cut(s) 755, 932, 1406, 3528
BsmI GAATGC 6 cut(s) 1620, 2753, 3668, 3862, 4123, 4843
BsnI GGCC 7 cut(s) 1229, 2444, 2558, 2795, 3055, 3264, 3452
Bso31I GGTCTC 1 cut(s) 3897
BsoBI CYCGRG 1 cut(s) 4254
Bsp1286I GDGCHC 8 cut(s) 64, 408, 648, 1122, 1543, 2536, 2962, 3096
Bsp13I TCCGGA 1 cut(s) 4706
Bsp1407I TGTACA 2 cut(s) 340, 4227
Bsp19I CCATGG 2 cut(s) 1777, 4671
BspACI CCGC 7 cut(s) 520, 1514, 2563, 3634, 4577, 5047, 5074
BspANI GGCC 7 cut(s) 1229, 2444, 2558, 2795, 3055, 3264, 3452
BspDI ATCGAT 1 cut(s) 4899
BspEI TCCGGA 1 cut(s) 4706
BspFNI CGCG 1 cut(s) 1516
BspHI TCATGA 3 cut(s) 1158, 4909, 5311
BspLI GGNNCC 9 cut(s) 61, 240, 518, 1676, 1998, 2157, 2323, 3980, 5095
BspMAI CTGCAG 4 cut(s) 874, 1933, 2064, 3787
BspMI ACCTGC 2 cut(s) 2474, 4292
BspQI GCTCTTC 2 cut(s) 549, 3504
BspT107I GGYRCC 2 cut(s) 1674, 2321
BspTI CTTAAG 1 cut(s) 3134
BspTNI GGTCTC 1 cut(s) 3897
BsrBI CCGCTC 1 cut(s) 522
BsrDI GCAATG 2 cut(s) 701, 2253
BsrFI RCCGGY 1 cut(s) 4438
BsrGI TGTACA 2 cut(s) 340, 4227
BsrI ACTGG 9 cut(s) 47, 2867, 2971, 3888, 4022, 4374, 4729, 4802, 5151
BssAI RCCGGY 1 cut(s) 4438
BssNAI GTATAC 1 cut(s) 1421
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 7 cut(s) 1035, 1777, 2387, 2445, 4139, 4671, 5067
Bst1107I GTATAC 1 cut(s) 1421
Bst2BI CACGAG 1 cut(s) 1020
Bst2UI CCWGG 5 cut(s) 2413, 2792, 3130, 3428, 3875
Bst6I CTCTTC 4 cut(s) 149, 549, 1998, 3504
BstAFI CTTAAG 1 cut(s) 3134
BstAPI GCANNNNNTGC 3 cut(s) 475, 2897, 5168
BstAUI TGTACA 2 cut(s) 340, 4227
BstBAI YACGTR 1 cut(s) 860
BstDSI CCRYGG 3 cut(s) 1777, 2552, 4671
BstFNI CGCG 1 cut(s) 1516
BstHHI GCGC 3 cut(s) 296, 4123, 4376
BstMAI GTCTC 9 cut(s) 869, 1036, 1322, 1855, 2034, 3897, 4560, 4870, 5026
BstMCI CGRYCG 1 cut(s) 2078
BstNI CCWGG 5 cut(s) 2413, 2792, 3130, 3428, 3875
BstNSI RCATGY 9 cut(s) 347, 2195, 2635, 2753, 3586, 4247, 4450, 4623, 5188
BstSCI CCNGG 6 cut(s) 1447, 2411, 2790, 3128, 3426, 3873
BstSFI CTRYAG 8 cut(s) 870, 972, 1929, 2060, 3439, 3657, 3783, 4124
BstSLI GKGCMC 3 cut(s) 2536, 2962, 3096
BstUI CGCG 1 cut(s) 1516
BstV2I GAAGAC 1 cut(s) 2120
BstX2I RGATCY 6 cut(s) 97, 1744, 2718, 3175, 4703, 4867
BstXI CCANNNNNNTGG 1 cut(s) 289
BstYI RGATCY 6 cut(s) 97, 1744, 2718, 3175, 4703, 4867
BstZ17I GTATAC 1 cut(s) 1421
Bsu15I ATCGAT 1 cut(s) 4899
Bsu36I CCTNAGG 2 cut(s) 1950, 2765
BsuI GTATCC 2 cut(s) 3000, 3424
BsuRI GGCC 7 cut(s) 1229, 2444, 2558, 2795, 3055, 3264, 3452
BsuTUI ATCGAT 1 cut(s) 4899
BtgI CCRYGG 3 cut(s) 1777, 2552, 4671
BtgZI GCGATG 2 cut(s) 447, 2126
BtsI GCAGTG 3 cut(s) 2678, 2895, 4000
BveI ACCTGC 2 cut(s) 2474, 4292
CaiI CAGNNNCTG 2 cut(s) 2525, 3968
CciI TCATGA 3 cut(s) 1158, 4909, 5311
CfoI GCGC 3 cut(s) 296, 4123, 4376
Cfr10I RCCGGY 1 cut(s) 4438
ClaI ATCGAT 1 cut(s) 4899
CseI GACGC 1 cut(s) 3745
Csp6I GTAC 8 cut(s) 341, 1838, 2747, 2906, 3811, 4228, 4832, 5300
CviQI GTAC 8 cut(s) 341, 1838, 2747, 2906, 3811, 4228, 4832, 5300
DraIII CACNNNGTG 1 cut(s) 1681
DrdI GACNNNNNNGTC 1 cut(s) 3352
DseDI GACNNNNNNGTC 1 cut(s) 3352
EaeI YGGCCR 4 cut(s) 1227, 2442, 2793, 3450
Eam1104I CTCTTC 4 cut(s) 149, 549, 1998, 3504
EarI CTCTTC 4 cut(s) 149, 549, 1998, 3504
Ecl136II GAGCTC 2 cut(s) 646, 1541
Eco130I CCWWGG 7 cut(s) 1035, 1777, 2387, 2445, 4139, 4671, 5067
Eco147I AGGCCT 1 cut(s) 3264
Eco24I GRGCYC 3 cut(s) 64, 648, 1543
Eco31I GGTCTC 1 cut(s) 3897
Eco47I GGWCC 8 cut(s) 239, 608, 1263, 1471, 3577, 3886, 5071, 5327
Eco53kI GAGCTC 2 cut(s) 646, 1541
Eco57I CTGAAG 6 cut(s) 1146, 1302, 1587, 1807, 2547, 5285
Eco81I CCTNAGG 2 cut(s) 1950, 2765
Eco88I CYCGRG 1 cut(s) 4254
EcoICRI GAGCTC 2 cut(s) 646, 1541
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 5 cut(s) 2411, 2790, 3128, 3426, 3873
EcoT14I CCWWGG 7 cut(s) 1035, 1777, 2387, 2445, 4139, 4671, 5067
EcoT22I ATGCAT 1 cut(s) 2755
EcoT38I GRGCYC 3 cut(s) 64, 648, 1543
ErhI CCWWGG 7 cut(s) 1035, 1777, 2387, 2445, 4139, 4671, 5067
Esp3I CGTCTC 1 cut(s) 4560
FaqI GGGAC 4 cut(s) 755, 932, 1406, 3528
FauI CCCGC 1 cut(s) 5054
FauNDI CATATG 1 cut(s) 538
FbaI TGATCA 3 cut(s) 625, 766, 4354
FblI GTMKAC 2 cut(s) 1420, 4986
FriOI GRGCYC 3 cut(s) 64, 648, 1543
GlaI GCGC 3 cut(s) 295, 4122, 4375
GsuI CTGGAG 7 cut(s) 1557, 2238, 3183, 3857, 4019, 4655, 4746
HaeIII GGCC 7 cut(s) 1229, 2444, 2558, 2795, 3055, 3264, 3452
HapII CCGG 5 cut(s) 1448, 3724, 3889, 4439, 4707
HgaI GACGC 1 cut(s) 3745
HhaI GCGC 3 cut(s) 296, 4123, 4376
Hin6I GCGC 3 cut(s) 294, 4121, 4374
HinP1I GCGC 3 cut(s) 294, 4121, 4374
HincII GTYRAC 3 cut(s) 837, 2403, 4987
HindII GTYRAC 3 cut(s) 837, 2403, 4987
HindIII AAGCTT 5 cut(s) 1599, 3100, 3678, 4919, 4958
HpaI GTTAAC 1 cut(s) 2403
HpaII CCGG 5 cut(s) 1448, 3724, 3889, 4439, 4707
HphI GGTGA 5 cut(s) 632, 1338, 2134, 2639, 3494
Hpy99I CGWCG 1 cut(s) 2913
HpyCH4IV ACGT 3 cut(s) 859, 1479, 2908
HpySE526I ACGT 3 cut(s) 859, 1479, 2908
HspAI GCGC 3 cut(s) 294, 4121, 4374
Kpn2I TCCGGA 1 cut(s) 4706
Ksp22I TGATCA 3 cut(s) 625, 766, 4354
KspAI GTTAAC 1 cut(s) 2403
LguI GCTCTTC 2 cut(s) 549, 3504
MaeII ACGT 3 cut(s) 859, 1479, 2908
MaeIII GTNAC 9 cut(s) 376, 413, 1425, 2627, 2702, 3482, 3645, 4333, 4793
MbiI CCGCTC 1 cut(s) 522
MfeI CAATTG 2 cut(s) 2274, 5051
MflI RGATCY 6 cut(s) 97, 1744, 2718, 3175, 4703, 4867
MhlI GDGCHC 8 cut(s) 64, 408, 648, 1122, 1543, 2536, 2962, 3096
MlsI TGGCCA 3 cut(s) 1229, 2444, 2795
MluNI TGGCCA 3 cut(s) 1229, 2444, 2795
MmeI TCCRAC 7 cut(s) 404, 1351, 1695, 2637, 3731, 4760, 5354
Mox20I TGGCCA 3 cut(s) 1229, 2444, 2795
Mph1103I ATGCAT 1 cut(s) 2755
MroI TCCGGA 1 cut(s) 4706
MroXI GAANNNNTTC 7 cut(s) 1342, 1903, 2285, 2298, 2979, 3498, 5384
MscI TGGCCA 3 cut(s) 1229, 2444, 2795
MslI CAYNNNNRTG 8 cut(s) 348, 411, 846, 2319, 3624, 3934, 4267, 5316
Msp20I TGGCCA 3 cut(s) 1229, 2444, 2795
MspA1I CMGCKG 1 cut(s) 1237
MspCI CTTAAG 1 cut(s) 3134
MspI CCGG 5 cut(s) 1448, 3724, 3889, 4439, 4707
MspR9I CCNGG 6 cut(s) 1449, 2413, 2792, 3130, 3428, 3875
MunI CAATTG 2 cut(s) 2274, 5051
Mva1269I GAATGC 6 cut(s) 1620, 2753, 3668, 3862, 4123, 4843
MvaI CCWGG 5 cut(s) 2413, 2792, 3130, 3428, 3875
MvnI CGCG 1 cut(s) 1516
NciI CCSGG 1 cut(s) 1449
NcoI CCATGG 2 cut(s) 1777, 4671
NdeI CATATG 1 cut(s) 538
NlaIV GGNNCC 9 cut(s) 61, 240, 518, 1676, 1998, 2157, 2323, 3980, 5095
NmuCI GTSAC 6 cut(s) 376, 413, 2627, 2702, 3482, 4793
NsiI ATGCAT 1 cut(s) 2755
NspI RCATGY 9 cut(s) 347, 2195, 2635, 2753, 3586, 4247, 4450, 4623, 5188
OliI CACNNNNGTG 1 cut(s) 411
PacI TTAATTAA 1 cut(s) 4413
PaeR7I CTCGAG 1 cut(s) 4254
PagI TCATGA 3 cut(s) 1158, 4909, 5311
PceI AGGCCT 1 cut(s) 3264
PciI ACATGT 1 cut(s) 4619
PciSI GCTCTTC 2 cut(s) 549, 3504
PcsI WCGNNNNNNNCGW 1 cut(s) 2742
PctI GAATGC 6 cut(s) 1620, 2753, 3668, 3862, 4123, 4843
PdmI GAANNNNTTC 7 cut(s) 1342, 1903, 2285, 2298, 2979, 3498, 5384
PflFI GACNNNGTC 1 cut(s) 2653
PflMI CCANNNNNTGG 1 cut(s) 1480
PfoI TCCNGGA 4 cut(s) 1447, 2411, 3426, 3873
Ple19I CGATCG 1 cut(s) 2078
Ppu21I YACGTR 1 cut(s) 860
PpuMI RGGWCCY 1 cut(s) 239
PscI ACATGT 1 cut(s) 4619
PshBI ATTAAT 1 cut(s) 4409
PsiI TTATAA 2 cut(s) 1329, 1665
Psp124BI GAGCTC 2 cut(s) 648, 1543
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 5 cut(s) 2411, 2790, 3128, 3426, 3873
PspGI CCWGG 5 cut(s) 2411, 2790, 3128, 3426, 3873
PspN4I GGNNCC 9 cut(s) 61, 240, 518, 1676, 1998, 2157, 2323, 3980, 5095
PspPPI RGGWCCY 1 cut(s) 239
PsrI GAACNNNNNNTAC 4 cut(s) 2139, 2171, 2629, 2661
PstI CTGCAG 4 cut(s) 874, 1933, 2064, 3787
PstNI CAGNNNCTG 2 cut(s) 2525, 3968
PsuI RGATCY 6 cut(s) 97, 1744, 2718, 3175, 4703, 4867
PsyI GACNNNGTC 1 cut(s) 2653
PvuI CGATCG 1 cut(s) 2078
PvuII CAGCTG 1 cut(s) 1237
RsaI GTAC 8 cut(s) 342, 1839, 2748, 2907, 3812, 4229, 4833, 5301
RsaNI GTAC 8 cut(s) 341, 1838, 2747, 2906, 3811, 4228, 4832, 5300
RseI CAYNNNNRTG 8 cut(s) 348, 411, 846, 2319, 3624, 3934, 4267, 5316
SacI GAGCTC 2 cut(s) 648, 1543
SalI GTCGAC 1 cut(s) 4985
SapI GCTCTTC 2 cut(s) 549, 3504
ScaI AGTACT 2 cut(s) 1839, 5301
ScrFI CCNGG 6 cut(s) 1449, 2413, 2792, 3130, 3428, 3875
SduI GDGCHC 8 cut(s) 64, 408, 648, 1122, 1543, 2536, 2962, 3096
SfcI CTRYAG 8 cut(s) 870, 972, 1929, 2060, 3439, 3657, 3783, 4124
Sfr274I CTCGAG 1 cut(s) 4254
SinI GGWCC 8 cut(s) 239, 608, 1263, 1471, 3577, 3886, 5071, 5327
SlaI CTCGAG 1 cut(s) 4254
SmiMI CAYNNNNRTG 8 cut(s) 348, 411, 846, 2319, 3624, 3934, 4267, 5316
SmlI CTYRAG 5 cut(s) 401, 707, 3134, 4254, 4740
SmoI CTYRAG 5 cut(s) 401, 707, 3134, 4254, 4740
SseBI AGGCCT 1 cut(s) 3264
SsiI CCGC 7 cut(s) 520, 1514, 2563, 3634, 4577, 5047, 5074
SspI AATATT 2 cut(s) 69, 214
SstI GAGCTC 2 cut(s) 648, 1543
StuI AGGCCT 1 cut(s) 3264
StyD4I CCNGG 6 cut(s) 1447, 2411, 2790, 3128, 3426, 3873
StyI CCWWGG 7 cut(s) 1035, 1777, 2387, 2445, 4139, 4671, 5067
TaiI ACGT 3 cut(s) 862, 1482, 2911
TaqI TCGA 9 cut(s) 816, 2648, 3919, 4255, 4763, 4770, 4899, 4986, 5177
TatI WGTACW 5 cut(s) 340, 1837, 4227, 4831, 5299
TseFI GTSAC 6 cut(s) 376, 413, 2627, 2702, 3482, 4793
Tsp45I GTSAC 6 cut(s) 376, 413, 2627, 2702, 3482, 4793
TspGWI ACGGA 5 cut(s) 146, 1662, 3224, 4352, 5216
Tth111I GACNNNGTC 1 cut(s) 2653
Van91I CCANNNNNTGG 1 cut(s) 1480
Vha464I CTTAAG 1 cut(s) 3134
VneI GTGCAC 3 cut(s) 2532, 2958, 3092
VpaK11BI GGWCC 8 cut(s) 239, 608, 1263, 1471, 3577, 3886, 5071, 5327
VspI ATTAAT 1 cut(s) 4409
XapI RAATTY 6 cut(s) 1289, 1452, 2639, 2975, 3947, 4389
XbaI TCTAGA 1 cut(s) 3248
XceI RCATGY 9 cut(s) 347, 2195, 2635, 2753, 3586, 4247, 4450, 4623, 5188
XcmI CCANNNNNNNNNTGG 1 cut(s) 5074
XhoI CTCGAG 1 cut(s) 4254
XmaJI CCTAGG 2 cut(s) 2387, 4139
XmiI GTMKAC 2 cut(s) 1420, 4986
XmnI GAANNNNTTC 7 cut(s) 1342, 1903, 2285, 2298, 2979, 3498, 5384
ZrmI AGTACT 2 cut(s) 1839, 5301
Zsp2I ATGCAT 1 cut(s) 2755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.