Rh2BG682000

DUF4210

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001019
Physical Location & Seq
Forward (+)
89111879 .. 89117521
5643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG682000.1

Sequence Viewer

Length: 1590 bp
ATGGGATTGCCTCAACTTCCCACAAGTGGAACCTCTGAGGAGGTACAATCAGGATCTTTCGGCTCATTTTTACAAAGCCCGCCTCGATCTTCTGGGATGAGCGCATGTGATTTGGATGTAATGCATGTTGAGAGTGTAAGCAGAATGGGCGGAAATTCATTATGCTCCTCTTTAGGAGAATACAAAGCAAGAAGCCCGGTAGAACTGTCAAAGTTCCCTGATGATGATTCATTTCGGTTTAGAGGGAATGGTTCTCTCAGTAAAGCTGGTCATAACACTCCCAGAAATGGACGATATATTCAGACCCCTGCTTCTAGGACTGTGGGTTTTGAAGCAAGAAGAACAAGTCTAAATGATGGTTCCAACAGTGTTTTATCTGATCACGTGCACTCTTCATCTGCAGTTATTGCCACCGCCAATGAAATTGCGAGCAGTGGGTCATTAGTGAAGAAGAGAATGCACTCCCCATTCAAGTCCCTATCAGCAGACCAATTTAATGGTGAGCATTTGGACCTTGCTTGCAGAACTGATCGAACAAATACTCCTGCTGTCAGTGATAGTAACAGTACATCACATGATCATAAGAAACCAAATGTTGGAAGTGAAAACCATTTTACCATTCCAGCTCGGTCCGAGCAGAAGAGCTTACCCTGTGATAATGGTAGTATGGCTTCCATTTTCACTGACGGCCCTTTAGTGGTCATAAATCATCCCCCACCTAATCTTTTGTATTTACCATCTCCTAGATGTGAAGTGAGAGAGTTAAAAGTCCAAAGTGAGCCAATATCAATATCTCCGAAAGAAGTGATTTTACCTCAGCTTTCTTTGTCACCTCTTGGCCGCAAGGTGTCTGAAAGAGGGAAATCTGCAGGGAGGTGCAGCAACAGTACTAATGAAGAGTTGGAGGATCGTCATTTGACGTTGAGGAATGTAAAAGAAGCAAGCAAAAAGTCTAAATTAGGGAGTCTGTTTTCCCCTGAAGAAGATGAGTTTCAGATAACAAGTAAATCATTTGAAGAGGAAGAGTTTGATATATTCCACAAAGAGTTTTGTCCATCTTCTGTGGAAACTGATACTCCTTTAAGTTATTCCTTACCCCAAGGGTTATCCCCCACCTCTCAATGCATGAGATTCGTTAGAAATCTAAGTGGGCTTTCAGTTAGGAGATCCTTGGTTGGTTCGTTTGAAGAATCTCTACTGTCTGGTCGGTTCTTATCAGGGAAACTCAGTCAGAGAATTGATGGTTTTCTGGGTGTACTAAGCGTTACTGGGGGAAACTTCTCACCACGATCACAGAAGCTTCCTTTTTCAGTAACTAGTGTAGAGGGAGATTGCTACTTATTGTATTATGCATCCATATATCTTGCGGGAAATTCGGCCTTGAATAGGAGTGGAGGACAAAAGCTGAAACGGGGACTTAGTAATGATGATTCACAGATTGTCAAGAGCCGCCTGCGCATTCCTGTCAAGGGTCGTATACAACTGGTTCTAAGTAATCCTGAAAAGACACCAATTCATACTTTCCTTTGCAACTATGATTTAAGTGACATGCCGGCTGGTACCAAAGTAAGAGGAATCTTTCCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromosome_seg DUF4210

Protein Analysis

529

Amino Acids

57.64

Weight (kDa)

8.58

Isoelectric Point (pI)

56.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1457
Acc65I GGTACC 1 cut(s) 1559
AccB1I GGYRCC 1 cut(s) 1559
AccB7I CCANNNNNTGG 1 cut(s) 596
AccI GTMKAC 1 cut(s) 1477
AciI CCGC 6 cut(s) 80, 150, 414, 841, 1367, 1450
AclWI GGATC 3 cut(s) 61, 915, 1161
AcoI YGGCCR 1 cut(s) 838
AcsI RAATTY 2 cut(s) 154, 1372
AcuI CTGAAG 1 cut(s) 999
AcvI CACGTG 1 cut(s) 385
AfaI GTAC 5 cut(s) 45, 568, 889, 1257, 1561
AfiI CCNNNNNNNGG 6 cut(s) 26, 287, 596, 697, 1386, 1469
AgsI TTSAA 5 cut(s) 332, 472, 1016, 1187, 1384
AhlI ACTAGT 1 cut(s) 1316
AloI GAACNNNNNNTCC 2 cut(s) 526, 558
AluBI AGCT 6 cut(s) 266, 626, 645, 820, 1300, 1405
AluI AGCT 6 cut(s) 266, 626, 645, 820, 1300, 1405
Alw21I GWGCWC 1 cut(s) 390
Alw44I GTGCAC 1 cut(s) 386
AlwI GGATC 3 cut(s) 61, 915, 1161
AoxI GGCC 3 cut(s) 688, 838, 1377
ApaLI GTGCAC 1 cut(s) 386
ApeKI GCWGC 1 cut(s) 879
ApoI RAATTY 2 cut(s) 154, 1372
Asp718I GGTACC 1 cut(s) 1559
AspLEI GCGC 2 cut(s) 104, 1458
AspS9I GGNCC 3 cut(s) 511, 630, 689
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 3 cut(s) 512, 822, 1275
AvaII GGWCC 2 cut(s) 511, 630
BaeGI GKGCMC 1 cut(s) 390
BaeI ACNNNNGTAYC 2 cut(s) 1551, 1584
BanI GGYRCC 1 cut(s) 1559
BarI GAAGNNNNNNTAC 6 cut(s) 655, 687, 795, 827, 1179, 1211
BbrPI CACGTG 1 cut(s) 385
Bbv12I GWGCWC 1 cut(s) 390
BbvCI CCTCAGC 1 cut(s) 816
BbvI GCAGC 1 cut(s) 891
BccI CCATC 4 cut(s) 350, 745, 1063, 1235
BceAI ACGGC 1 cut(s) 703
BcgI CGANNNNNNTGC 2 cut(s) 1114, 1148
BclI TGATCA 2 cut(s) 379, 577
BcnI CCSGG 1 cut(s) 197
BcuI ACTAGT 1 cut(s) 1316
BfaI CTAG 3 cut(s) 315, 744, 1317
BfmI CTRYAG 2 cut(s) 399, 867
BisI GCNGC 3 cut(s) 841, 880, 1450
BlsI GCNGC 3 cut(s) 842, 881, 1451
BmcAI AGTACT 1 cut(s) 889
Bme1390I CCNGG 1 cut(s) 197
Bme18I GGWCC 2 cut(s) 511, 630
BmgT120I GGNCC 3 cut(s) 511, 630, 689
BmiI GGNNCC 3 cut(s) 31, 361, 1561
BmrFI CCNGG 1 cut(s) 197
BmrI ACTGGG 1 cut(s) 1278
BmsI GCATC 1 cut(s) 1361
BmuI ACTGGG 1 cut(s) 1278
Bpu10I CCTNAGC 1 cut(s) 816
BpuMI CCSGG 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 385
BsaJI CCNNGG 2 cut(s) 1099, 1170
BsaXI ACNNNNNCTCC 4 cut(s) 526, 556, 1060, 1090
Bsc4I CCNNNNNNNGG 6 cut(s) 26, 287, 596, 697, 1386, 1469
Bse118I RCCGGY 1 cut(s) 1552
Bse1I ACTGG 2 cut(s) 1273, 1488
BseDI CCNNGG 2 cut(s) 1099, 1170
BseGI GGATG 4 cut(s) 102, 121, 709, 1352
BseLI CCNNNNNNNGG 6 cut(s) 26, 287, 596, 697, 1386, 1469
BseMII CTCAG 4 cut(s) 27, 271, 830, 1240
BseNI ACTGG 2 cut(s) 1273, 1488
BseRI GAGGAG 2 cut(s) 53, 157
BseSI GKGCMC 1 cut(s) 390
BseXI GCAGC 1 cut(s) 891
BsgI GTGCAG 1 cut(s) 898
BshFI GGCC 3 cut(s) 690, 840, 1379
BshNI GGYRCC 1 cut(s) 1559
BsiHKAI GWGCWC 1 cut(s) 390
BsiSI CCGG 2 cut(s) 197, 1553
BslFI GGGAC 2 cut(s) 460, 1428
BslI CCNNNNNNNGG 6 cut(s) 26, 287, 596, 697, 1386, 1469
BsmFI GGGAC 2 cut(s) 460, 1428
BsmI GAATGC 2 cut(s) 462, 1458
BsnI GGCC 3 cut(s) 690, 840, 1379
Bsp1286I GDGCHC 1 cut(s) 390
Bsp143I GATC 8 cut(s) 53, 86, 379, 529, 577, 907, 1166, 1289
BspACI CCGC 6 cut(s) 80, 150, 414, 841, 1367, 1450
BspANI GGCC 3 cut(s) 690, 840, 1379
BspCNI CTCAG 4 cut(s) 28, 270, 829, 1239
BspLI GGNNCC 3 cut(s) 31, 361, 1561
BspMAI CTGCAG 2 cut(s) 403, 871
BspPI GGATC 3 cut(s) 61, 915, 1161
BspQI GCTCTTC 1 cut(s) 635
BspT107I GGYRCC 1 cut(s) 1559
BsrFI RCCGGY 1 cut(s) 1552
BsrI ACTGG 2 cut(s) 1273, 1488
BssAI RCCGGY 1 cut(s) 1552
BssECI CCNNGG 2 cut(s) 1099, 1170
BssMI GATC 8 cut(s) 53, 86, 379, 529, 577, 907, 1166, 1289
BssNAI GTATAC 1 cut(s) 1478
BssT1I CCWWGG 2 cut(s) 1099, 1170
Bst1107I GTATAC 1 cut(s) 1478
Bst4CI ACNGT 6 cut(s) 207, 322, 368, 566, 887, 1200
Bst6I CTCTTC 6 cut(s) 397, 446, 635, 891, 1011, 1017
BstAPI GCANNNNNTGC 1 cut(s) 407
BstBAI YACGTR 1 cut(s) 385
BstC8I GCNNGC 6 cut(s) 80, 430, 520, 943, 1454, 1554
BstDEI CTNAG 8 cut(s) 36, 257, 816, 1145, 1226, 1259, 1418, 1490
BstENI CCTNNNNNAGG 1 cut(s) 1384
BstF5I GGATG 4 cut(s) 102, 121, 709, 1352
BstHHI GCGC 2 cut(s) 104, 1458
BstKTI GATC 8 cut(s) 56, 89, 382, 532, 580, 910, 1169, 1292
BstMBI GATC 8 cut(s) 53, 86, 379, 529, 577, 907, 1166, 1289
BstMWI GCNNNNNNNGC 3 cut(s) 147, 407, 1455
BstNSI RCATGY 3 cut(s) 108, 128, 1552
BstSCI CCNGG 1 cut(s) 195
BstSFI CTRYAG 2 cut(s) 399, 867
BstSLI GKGCMC 1 cut(s) 390
BstV1I GCAGC 1 cut(s) 891
BstX2I RGATCY 2 cut(s) 53, 1166
BstXI CCANNNNNNTGG 1 cut(s) 497
BstYI RGATCY 2 cut(s) 53, 1166
BstZ17I GTATAC 1 cut(s) 1478
BsuRI GGCC 3 cut(s) 690, 840, 1379
BtsCI GGATG 4 cut(s) 102, 121, 709, 1352
BtsI GCAGTG 1 cut(s) 439
BtsIMutI CAGTG 4 cut(s) 373, 439, 559, 681
Cac8I GCNNGC 6 cut(s) 80, 430, 520, 943, 1454, 1554
CfoI GCGC 2 cut(s) 104, 1458
Cfr10I RCCGGY 1 cut(s) 1552
Cfr13I GGNCC 3 cut(s) 511, 630, 689
CpoI CGGWCCG 1 cut(s) 630
Csp6I GTAC 5 cut(s) 44, 567, 888, 1256, 1560
CspI CGGWCCG 1 cut(s) 630
CviAII CATG 5 cut(s) 105, 125, 575, 1126, 1549
CviQI GTAC 5 cut(s) 44, 567, 888, 1256, 1560
DdeI CTNAG 8 cut(s) 36, 257, 816, 1145, 1226, 1259, 1418, 1490
DpnI GATC 8 cut(s) 55, 88, 381, 531, 579, 909, 1168, 1291
DpnII GATC 8 cut(s) 53, 86, 379, 529, 577, 907, 1166, 1289
EaeI YGGCCR 1 cut(s) 838
Eam1104I CTCTTC 6 cut(s) 397, 446, 635, 891, 1011, 1017
EarI CTCTTC 6 cut(s) 397, 446, 635, 891, 1011, 1017
EciI GGCGGA 1 cut(s) 165
Eco130I CCWWGG 2 cut(s) 1099, 1170
Eco47I GGWCC 2 cut(s) 511, 630
Eco57I CTGAAG 1 cut(s) 999
Eco72I CACGTG 1 cut(s) 385
EcoNI CCTNNNNNAGG 1 cut(s) 1384
EcoT14I CCWWGG 2 cut(s) 1099, 1170
EcoT22I ATGCAT 3 cut(s) 126, 1127, 1354
ErhI CCWWGG 2 cut(s) 1099, 1170
FaeI CATG 5 cut(s) 108, 128, 578, 1129, 1552
FaqI GGGAC 2 cut(s) 460, 1428
FatI CATG 5 cut(s) 104, 124, 574, 1125, 1548
FauI CCCGC 2 cut(s) 87, 1360
FbaI TGATCA 2 cut(s) 379, 577
FblI GTMKAC 1 cut(s) 1477
Fnu4HI GCNGC 3 cut(s) 841, 880, 1450
FokI GGATG 4 cut(s) 109, 128, 696, 1339
Fsp4HI GCNGC 3 cut(s) 841, 880, 1450
FspBI CTAG 3 cut(s) 315, 744, 1317
FspI TGCGCA 1 cut(s) 1457
GlaI GCGC 2 cut(s) 103, 1457
GluI GCNGC 3 cut(s) 841, 880, 1450
HaeIII GGCC 3 cut(s) 690, 840, 1379
HapII CCGG 2 cut(s) 197, 1553
HhaI GCGC 2 cut(s) 104, 1458
Hin1II CATG 5 cut(s) 108, 128, 578, 1129, 1552
Hin6I GCGC 2 cut(s) 102, 1456
HinP1I GCGC 2 cut(s) 102, 1456
HindIII AAGCTT 1 cut(s) 1298
HinfI GANTC 6 cut(s) 227, 964, 1131, 1190, 1430, 1575
HpaII CCGG 2 cut(s) 197, 1553
HphI GGTGA 3 cut(s) 512, 822, 1275
Hpy166II GTNNAC 3 cut(s) 388, 1256, 1478
Hpy188I TCNGA 8 cut(s) 37, 303, 379, 634, 798, 853, 996, 1233
Hpy188III TCNNGA 3 cut(s) 51, 1444, 1499
Hpy8I GTNNAC 3 cut(s) 388, 1256, 1478
HpyCH4III ACNGT 6 cut(s) 207, 322, 368, 566, 887, 1200
HpyCH4IV ACGT 2 cut(s) 384, 920
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 407, 1455
HpyF3I CTNAG 8 cut(s) 36, 257, 816, 1145, 1226, 1259, 1418, 1490
HpySE526I ACGT 2 cut(s) 384, 920
Hsp92II CATG 5 cut(s) 108, 128, 578, 1129, 1552
HspAI GCGC 2 cut(s) 102, 1456
KpnI GGTACC 1 cut(s) 1563
KroI GCCGGC 1 cut(s) 1552
KroNI GCCGGC 1 cut(s) 1554
Ksp22I TGATCA 2 cut(s) 379, 577
Kzo9I GATC 8 cut(s) 53, 86, 379, 529, 577, 907, 1166, 1289
LguI GCTCTTC 1 cut(s) 635
LmnI GCTCC 1 cut(s) 170
Lsp1109I GCAGC 1 cut(s) 891
LweI GCATC 1 cut(s) 1361
MaeI CTAG 3 cut(s) 315, 744, 1317
MaeII ACGT 2 cut(s) 384, 920
MaeIII GTNAC 5 cut(s) 560, 828, 1264, 1312, 1544
MalI GATC 8 cut(s) 55, 88, 381, 531, 579, 909, 1168, 1291
MboI GATC 8 cut(s) 53, 86, 379, 529, 577, 907, 1166, 1289
MflI RGATCY 2 cut(s) 53, 1166
MhlI GDGCHC 1 cut(s) 390
MluCI AATT 7 cut(s) 154, 423, 491, 956, 1236, 1372, 1512
MlyI GAGTC 1 cut(s) 973
MmeI TCCRAC 3 cut(s) 387, 577, 882
Mph1103I ATGCAT 3 cut(s) 126, 1127, 1354
MroNI GCCGGC 1 cut(s) 1552
MseI TTAA 5 cut(s) 495, 764, 1082, 1541, 1588
MspI CCGG 2 cut(s) 197, 1553
MspR9I CCNGG 1 cut(s) 197
Mva1269I GAATGC 2 cut(s) 462, 1458
MwoI GCNNNNNNNGC 3 cut(s) 147, 407, 1455
NaeI GCCGGC 1 cut(s) 1554
NciI CCSGG 1 cut(s) 197
NdeII GATC 8 cut(s) 53, 86, 379, 529, 577, 907, 1166, 1289
NgoMIV GCCGGC 1 cut(s) 1552
NlaIII CATG 5 cut(s) 108, 128, 578, 1129, 1552
NlaIV GGNNCC 3 cut(s) 31, 361, 1561
NmuCI GTSAC 2 cut(s) 828, 1544
NsbI TGCGCA 1 cut(s) 1457
NsiI ATGCAT 3 cut(s) 126, 1127, 1354
NspI RCATGY 3 cut(s) 108, 128, 1552
PciSI GCTCTTC 1 cut(s) 635
PctI GAATGC 2 cut(s) 462, 1458
PdiI GCCGGC 1 cut(s) 1554
PfeI GAWTC 5 cut(s) 227, 1131, 1190, 1430, 1575
PflMI CCANNNNNTGG 1 cut(s) 596
PkrI GCNGC 3 cut(s) 842, 881, 1451
PleI GAGTC 1 cut(s) 972
PmaCI CACGTG 1 cut(s) 385
PmlI CACGTG 1 cut(s) 385
PpsI GAGTC 1 cut(s) 972
Ppu21I YACGTR 1 cut(s) 385
PspCI CACGTG 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 31, 361, 1561
PspPI GGNCC 3 cut(s) 511, 630, 689
PstI CTGCAG 2 cut(s) 403, 871
PsuI RGATCY 2 cut(s) 53, 1166
RsaI GTAC 5 cut(s) 45, 568, 889, 1257, 1561
RsaNI GTAC 5 cut(s) 44, 567, 888, 1256, 1560
Rsr2I CGGWCCG 1 cut(s) 630
RsrII CGGWCCG 1 cut(s) 630
SapI GCTCTTC 1 cut(s) 635
SaqAI TTAA 5 cut(s) 495, 764, 1082, 1541, 1588
SatI GCNGC 3 cut(s) 841, 880, 1450
Sau3AI GATC 8 cut(s) 53, 86, 379, 529, 577, 907, 1166, 1289
Sau96I GGNCC 3 cut(s) 511, 630, 689
ScaI AGTACT 1 cut(s) 889
SchI GAGTC 1 cut(s) 973
ScrFI CCNGG 1 cut(s) 197
SduI GDGCHC 1 cut(s) 390
SfaNI GCATC 1 cut(s) 1361
SfcI CTRYAG 2 cut(s) 399, 867
SinI GGWCC 2 cut(s) 511, 630
SpeI ACTAGT 1 cut(s) 1316
Sse9I AATT 7 cut(s) 154, 423, 491, 956, 1236, 1372, 1512
SsiI CCGC 6 cut(s) 80, 150, 414, 841, 1367, 1450
SspMI CTAG 3 cut(s) 315, 744, 1317
StyD4I CCNGG 1 cut(s) 195
StyI CCWWGG 2 cut(s) 1099, 1170
TaaI ACNGT 6 cut(s) 207, 322, 368, 566, 887, 1200
TaiI ACGT 2 cut(s) 387, 923
TaqI TCGA 2 cut(s) 85, 532
TaqII GACCGA 1 cut(s) 618
TasI AATT 7 cut(s) 154, 423, 491, 956, 1236, 1372, 1512
TatI WGTACW 3 cut(s) 566, 887, 1255
TauI GCSGC 2 cut(s) 843, 1452
TfiI GAWTC 5 cut(s) 227, 1131, 1190, 1430, 1575
Tru1I TTAA 5 cut(s) 495, 764, 1082, 1541, 1588
Tru9I TTAA 5 cut(s) 495, 764, 1082, 1541, 1588
TscAI CASTG 4 cut(s) 373, 439, 559, 688
TseFI GTSAC 2 cut(s) 828, 1544
TseI GCWGC 1 cut(s) 879
Tsp45I GTSAC 2 cut(s) 828, 1544
TspDTI ATGAA 6 cut(s) 147, 219, 384, 435, 909, 1505
TspRI CASTG 4 cut(s) 373, 439, 559, 688
Van91I CCANNNNNTGG 1 cut(s) 596
VneI GTGCAC 1 cut(s) 386
VpaK11BI GGWCC 2 cut(s) 511, 630
XagI CCTNNNNNAGG 1 cut(s) 1384
XapI RAATTY 2 cut(s) 154, 1372
XceI RCATGY 3 cut(s) 108, 128, 1552
XmiI GTMKAC 1 cut(s) 1477
XspI CTAG 3 cut(s) 315, 744, 1317
ZrmI AGTACT 1 cut(s) 889
Zsp2I ATGCAT 3 cut(s) 126, 1127, 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.