Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001019

Physical Location & Seq

Genomic Coordinates Reverse (-)

446606 .. 450712

Gene Structure 4107 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1006000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2268 bp

ATGGGATTGCCTCAGGTGCCCTCAACTGGAACTTCTGAGGAGGTGCGAGCTGCTTGTCTGGGGTCCTTTTTACAAAGCCCGCCTCGATTTTCAAGTATGAGCACATGTGATATGGATGGGATGCTTGGTGGAAGTGCAAGCCAAGTGGGGGGGAATCCATTATGCTCCTCTTTAGGAGATTACCAGACAAGGACCTCCCTAGAATTTTCAAAGCTCCCTGATCATTCACTTGGATTTAGAGGGACCGCGCGAGTCACTAACAATGTTCATGGTTTGTCAATTGTTTCTGTTGATAAAGCTGGTCAGATCACCCCAAGAAATGGACAGAATGTTCTGACCCCTGCTTCTAGGATTGTAGGCTTTGAATCAAGCGGAACAAGTTCTCTAAATGATGATGACTCCAAGCGTGTTTCATGTGGGCACGTTCACTCCGCTTCTGCAGTTAATGTCACGACTAATGAAATTGTGACCAGTGGGTCTCGAGTCAGGAAGCGATTGTTGTCCTCATTGAATACTATGCTTTTTCCGCACCAGTTTGAAGGTGATCGTTTAAACATTGGTTGCACAACTTCTGGAGCAAATTATCCTGCTGTGAGTGATAATTTTAGTACGTCTGTCGCACATGATTATAAGAAAGCAAATGTTGGGAGCAAACGTAGTTTTACCATGCCAACTTGGTCTCTATCTAGTTGCTTTGAGCAGAAGAGCATGGCACATGATAGTGGTATTATGGAATCCATTTTTTTCACGAATGGCCCTTTGCTGGAAAACAAGGATATCGGACCTCGAAATAGTTATCAATCTGCACCTAGACGTGATGAGTTGAGAAAGCGGAGTAATGCAAAAATCCAAAGTGGGGCAATATCCACATCTCCAAAGGAAGTGATCTCACCTTTGCATCCTTTGTCACCTCTTGGCCCCAGGTTGTCTGAAAGATTGAAAATTGCAGGGGGCTGCAGGACTACTGACAAAGAGTTGGAGGATTGTCATTCAACCTTAAGGAACGTGAAACAAACACTTGACAAGTCTGATGCAGACATTCTCTTTTCCCCTGAACTAGGGGAGTTTAAGATTGCTAGTAAATTATTGGAAGATGTTGATGTTTTTCACAGGGAATTCCTTCCATCTTCTCTAGAGAATAGTACTGGCATAAGTTATTCTTTGTCACACGGGTCAGCTCCCAACTCCCAATGCATGAGTGGGCTTTCTGTCGGGAGATCCTTGGTTGGTTCATTCGAAGAATCTCTACTGTCTGGTCGGTTCATATCAGGGAAACCCAGTCAGAGAATCAATGGTTTTCTGGCTGTGCTAAGCATCACTGGGGGAAACTTTTCTCCGCAATCACAGAAGCTTCCTTTTTCAGTAACTAGTGTAGAAGGTGATTGCTATTTATTGTATTGCGCATCCATACATCTTTCGAGAAATTCAGCATTGAATAGGAGTGGAGTACAAAAGATTAAACGAGGCCTCAGCAATGATGGTTCACCGATTGTCACAAGCCGCCTGCGCATTCCTGTGAAGGGCCGTATACAATTGGTTCTAAGCAATCCAGAGAAGACACCTATTCATACCTTTCTTTGTAACTACGATTTAAGCGACATGCCAGCTGGTAGCAAAACTTTCTTACGCCAGAAGGTCACCTTAGCATGCAGCCCAAATTCTTCCCAGTCGGGACATGGGAAGACTGATCTGAACTCAAAAGTCATAGAGAAGAAGGAAGTAGAGAATACAGCCGGAGTAGACATTGTACATACAACAAGATCTGTGGATCAGAAAACTGAAATTAGAAGTGAAGACTCTAATTTTGTGGATTCAATAGATGAGGGGGATATCTCCAACTTATACCCTAAACTGGGAAGGGGATCCAATGCTTCGTTCCTTGTTGAGAAGAGCCTCAACAATGGGGAGTACCGAAGCAGCAATGGAAAGGAGCGCGAGTTTGCTGATGCATGCTATGGAACCGACAGGAAGCACGGACATGTTTGTTCAAGGTTCAATCAAAACAGTAATGGTGCTCTCCGCTATGCTCTCCATCTCCGTTTCACATGCCCTTTTCCCAAAAAAAGTTCTAGGACAGTCCAGAGGTGCAAATCAGATCCCCTATCTACACCACAGAGAACAGGCTTAGACATGGATGGGGAGCGCAGATTCTACCTGTACAATGACTTGAGGGTCGTGTTCCCTCAACGCCACACAGACGCTGATGAGGGAGAGTTGAACGTGGAATACCATTTCCCGGAGGATCCGAAATACTTCGACATCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Chromosome_seg DUF4210

Protein Analysis

756

Amino Acids

82.49

Weight (kDa)

8.61

Isoelectric Point (pI)

44.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Chromosome_seg	PF13889	672 - 754	1.3e-23	Chromosome segregation during meiosis

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001019)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26890 AT3G26890 AT3G26890 AT3G26890 AT3G26890 AT3G26890 AT3G26890 AT3G26890 AT5G41110 AT5G41110 AT5G41110
fragaria_vesca	FvH4_3g02970 FvH4_5g09080 FvH4_5g09080 FvH4_5g09080 FvH4_5g09080 FvH4_5g09080 FvH4_5g09080 FvH4_6g54040 FvH4_6g54040 FvH4_6g54040
malus_domestica	MD06G1134000.v1.1 MD09G1006000.v1.1 MD14G1150300.v1.1 MD17G1010000.v1.1
prunus_persica	Prupe.3G311000_v2.0.a1 Prupe.3G311000_v2.0.a1 Prupe.5G145000_v2.0.a1 Prupe.5G145000_v2.0.a1 Prupe.5G145000_v2.0.a1 Prupe.5G145000_v2.0.a1 Prupe.5G145000_v2.0.a1
pyrus_communis	pycom03g07800 pycom06g12520 pycom111g00440 pycom14g12490 pycom17g00450
rosa_chinensis	RchiOBHm_Chr2g0176221 RchiOBHm_Chr7g0189131
rosa_laevigata	RLG00000004630 RLG00000022392
rosa_multiflora	Rmu_co8469623.1_g000001 Rmu_sc0000405.1_g000014 Rmu_sc0007859.1_g000006
rosa_roxburghii	Rroxscaffold_2G00076670 Rroxscaffold_3G00265760
rosa_rugosa	Rorug02G0591900 Rorug02G0591900 Rorug02G0592000 Rorug06G0496100
rosa_samantha	Rh1DG179700 Rh2AG670500 Rh2BG682000 Rh2CG644800 Rh2DG695600 Rh7AG100900 Rh7BG103500 Rh7CG104900 Rh7DG102800
rosa_wichuraiana	Rw2G055000 Rw7G008710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	630
AasI	GACNNNNNNGTC	2 cut(s)	475, 2171
Acc16I	TGCGCA	2 cut(s)	1402, 1508
AccB1I	GGYRCC	1 cut(s)	16
AccB7I	CCANNNNNTGG	1 cut(s)	320
AccI	GTMKAC	2 cut(s)	1528, 1740
AccII	CGCG	3 cut(s)	248, 250, 1935
AciI	CCGC	9 cut(s)	80, 246, 372, 432, 527, 832, 1337, 1501, 2020
AclWI	GGATC	7 cut(s)	1212, 1776, 1857, 1870, 2090, 2237, 2250
AcsI	RAATTY	4 cut(s)	203, 1115, 1423, 1657
AfaI	GTAC	6 cut(s)	610, 1144, 1449, 1749, 1910, 2159
AflII	CTTAAG	1 cut(s)	997
AflIII	ACRYGT	2 cut(s)	104, 1978
AhlI	ACTAGT	1 cut(s)	1367
AjiI	CACGTC	1 cut(s)	815
AjnI	CCWGG	1 cut(s)	920
AjuI	GAANNNNNNNTTGG	2 cut(s)	1859, 1891
AloI	GAACNNNNNNTCC	2 cut(s)	315, 347
AluBI	AGCT	6 cut(s)	50, 214, 299, 1178, 1351, 1607
AluI	AGCT	6 cut(s)	50, 214, 299, 1178, 1351, 1607
Alw21I	GWGCWC	2 cut(s)	104, 2017
Alw26I	GTCTC	2 cut(s)	483, 684
AlwI	GGATC	7 cut(s)	1212, 1776, 1857, 1870, 2090, 2237, 2250
AlwNI	CAGNNNCTG	1 cut(s)	2201
Ama87I	CYCGRG	1 cut(s)	480
AoxI	GGCC	4 cut(s)	754, 916, 1465, 1522
ApeKI	GCWGC	4 cut(s)	50, 954, 1650, 1917
ApoI	RAATTY	4 cut(s)	203, 1115, 1423, 1657
ArsI	GACNNNNNNTTYG	2 cut(s)	1787, 1819
Asp700I	GAANNNNTTC	4 cut(s)	379, 1119, 1330, 2252
AspLEI	GCGC	5 cut(s)	250, 1403, 1509, 1935, 2145
AspS9I	GGNCC	7 cut(s)	63, 192, 243, 755, 782, 917, 1522
AsuC2I	CCSGG	1 cut(s)	2237
AsuHPI	GGTGA	7 cut(s)	301, 554, 882, 900, 1391, 1476, 1630
AsuII	TTCGAA	1 cut(s)	1236
AvaI	CYCGRG	1 cut(s)	480
AvaII	GGWCC	4 cut(s)	63, 192, 243, 782
AxyI	CCTNAGG	1 cut(s)	12
BaeGI	GKGCMC	2 cut(s)	21, 423
BamHI	GGATCC	2 cut(s)	1862, 2242
BanI	GGYRCC	1 cut(s)	16
BarI	GAAGNNNNNNTAC	2 cut(s)	1230, 1262
BbsI	GAAGAC	3 cut(s)	1562, 1688, 1800
Bbv12I	GWGCWC	2 cut(s)	104, 2017
BbvCI	CCTCAGC	1 cut(s)	1469
BbvI	GCAGC	4 cut(s)	37, 941, 1662, 1929
BccI	CCATC	5 cut(s)	110, 1132, 1472, 2040, 2129
BceAI	ACGGC	1 cut(s)	1509
BciT130I	CCWGG	1 cut(s)	922
BclI	TGATCA	1 cut(s)	220
BcnI	CCSGG	1 cut(s)	2237
BcoDI	GTCTC	2 cut(s)	483, 684
BcuI	ACTAGT	1 cut(s)	1367
BfaI	CTAG	9 cut(s)	200, 348, 687, 810, 1058, 1077, 1133, 1368, 2070
BfmI	CTRYAG	2 cut(s)	438, 955
BfrI	CTTAAG	1 cut(s)	997
BglII	AGATCT	1 cut(s)	1760
BisI	GCNGC	5 cut(s)	51, 955, 1501, 1651, 1918
BlpI	GCTNAGC	1 cut(s)	1310
BlsI	GCNGC	5 cut(s)	52, 956, 1502, 1652, 1919
BmcAI	AGTACT	1 cut(s)	1144
Bme1390I	CCNGG	2 cut(s)	922, 2237
Bme18I	GGWCC	4 cut(s)	63, 192, 243, 782
BmeT110I	CYCGRG	1 cut(s)	480
BmgBI	CACGTC	1 cut(s)	815
BmgT120I	GGNCC	7 cut(s)	63, 192, 243, 755, 782, 917, 1522
BmiI	GGNNCC	7 cut(s)	18, 64, 244, 919, 1864, 1960, 2244
BmrFI	CCNGG	2 cut(s)	922, 2237
BmrI	ACTGGG	4 cut(s)	1272, 1329, 1660, 1862
BmsI	GCATC	6 cut(s)	111, 907, 1021, 1323, 1412, 1936
BmuI	ACTGGG	4 cut(s)	1272, 1329, 1660, 1862
BpiI	GAAGAC	3 cut(s)	1562, 1688, 1800
BpmI	CTGGAG	1 cut(s)	594
Bpu10I	CCTNAGC	2 cut(s)	1469, 1642
Bpu1102I	GCTNAGC	1 cut(s)	1310
Bpu14I	TTCGAA	1 cut(s)	1236
BpuEI	CTTGAG	1 cut(s)	2188
BpuMI	CCSGG	1 cut(s)	2237
BsaI	GGTCTC	2 cut(s)	483, 684
BsaJI	CCNNGG	2 cut(s)	920, 1221
BsaXI	ACNNNNNCTCC	6 cut(s)	413, 443, 640, 670, 1922, 1952
Bse1I	ACTGG	8 cut(s)	31, 471, 532, 1150, 1278, 1324, 1666, 1857
Bse21I	CCTNAGG	1 cut(s)	12
Bse3DI	GCAATG	2 cut(s)	1480, 1927
BseBI	CCWGG	1 cut(s)	922
BseDI	CCNNGG	2 cut(s)	920, 1221
BseGI	GGATG	5 cut(s)	121, 126, 898, 1403, 2140
BseMI	GCAATG	2 cut(s)	1480, 1927
BseMII	CTCAG	3 cut(s)	26, 27, 1483
BseNI	ACTGG	8 cut(s)	31, 471, 532, 1150, 1278, 1324, 1666, 1857
BseRI	GAGGAG	2 cut(s)	53, 157
BseSI	GKGCMC	2 cut(s)	21, 423
BseXI	GCAGC	4 cut(s)	37, 941, 1662, 1929
BsgI	GTGCAG	1 cut(s)	789
Bsh1236I	CGCG	3 cut(s)	248, 250, 1935
BshFI	GGCC	4 cut(s)	756, 918, 1467, 1524
BshNI	GGYRCC	1 cut(s)	16
BsiHKAI	GWGCWC	2 cut(s)	104, 2017
BsiHKCI	CYCGRG	1 cut(s)	480
BsiSI	CCGG	2 cut(s)	1734, 2237
BslFI	GGGAC	2 cut(s)	256, 1686
BsmAI	GTCTC	2 cut(s)	483, 684
BsmFI	GGGAC	2 cut(s)	256, 1686
BsmI	GAATGC	1 cut(s)	1509
BsnI	GGCC	4 cut(s)	756, 918, 1467, 1524
Bso31I	GGTCTC	2 cut(s)	483, 684
BsoBI	CYCGRG	1 cut(s)	480
Bsp119I	TTCGAA	1 cut(s)	1236
Bsp1286I	GDGCHC	4 cut(s)	21, 104, 423, 2017
Bsp1407I	TGTACA	2 cut(s)	1747, 2157
Bsp1720I	GCTNAGC	1 cut(s)	1310
BspACI	CCGC	9 cut(s)	80, 246, 372, 432, 527, 832, 1337, 1501, 2020
BspANI	GGCC	4 cut(s)	756, 918, 1467, 1524
BspCNI	CTCAG	3 cut(s)	25, 28, 1482
BspFNI	CGCG	3 cut(s)	248, 250, 1935
BspLI	GGNNCC	7 cut(s)	18, 64, 244, 919, 1864, 1960, 2244
BspMAI	CTGCAG	2 cut(s)	442, 959
BspPI	GGATC	7 cut(s)	1212, 1776, 1857, 1870, 2090, 2237, 2250
BspQI	GCTCTTC	2 cut(s)	698, 1883
BspT104I	TTCGAA	1 cut(s)	1236
BspT107I	GGYRCC	1 cut(s)	16
BspTI	CTTAAG	1 cut(s)	997
BspTNI	GGTCTC	2 cut(s)	483, 684
BsrDI	GCAATG	2 cut(s)	1480, 1927
BsrGI	TGTACA	2 cut(s)	1747, 2157
BsrI	ACTGG	8 cut(s)	31, 471, 532, 1150, 1278, 1324, 1666, 1857
BssECI	CCNNGG	2 cut(s)	920, 1221
BssNAI	GTATAC	1 cut(s)	1529
BssT1I	CCWWGG	1 cut(s)	1221
Bst1107I	GTATAC	1 cut(s)	1529
Bst2UI	CCWGG	1 cut(s)	922
Bst4CI	ACNGT	3 cut(s)	1251, 2006, 2077
Bst6I	CTCTTC	2 cut(s)	698, 1883
BstAFI	CTTAAG	1 cut(s)	997
BstAUI	TGTACA	2 cut(s)	1747, 2157
BstBI	TTCGAA	1 cut(s)	1236
BstC8I	GCNNGC	7 cut(s)	48, 80, 139, 1505, 1605, 1648, 1951
BstDEI	CTNAG	7 cut(s)	12, 36, 1310, 1469, 1541, 1642, 2125
BstEII	GGTNACC	1 cut(s)	1636
BstENI	CCTNNNNNAGG	1 cut(s)	1056
BstF5I	GGATG	5 cut(s)	121, 126, 898, 1403, 2140
BstFNI	CGCG	3 cut(s)	248, 250, 1935
BstHHI	GCGC	5 cut(s)	250, 1403, 1509, 1935, 2145
BstMAI	GTCTC	2 cut(s)	483, 684
BstMWI	GCNNNNNNNGC	3 cut(s)	16, 526, 1506
BstNI	CCWGG	1 cut(s)	922
BstNSI	RCATGY	6 cut(s)	108, 1603, 1650, 1953, 1982, 2049
BstPI	GGTNACC	1 cut(s)	1636
BstSCI	CCNGG	2 cut(s)	920, 2235
BstSFI	CTRYAG	2 cut(s)	438, 955
BstSLI	GKGCMC	2 cut(s)	21, 423
BstUI	CGCG	3 cut(s)	248, 250, 1935
BstV1I	GCAGC	4 cut(s)	37, 941, 1662, 1929
BstV2I	GAAGAC	3 cut(s)	1562, 1688, 1800
BstX2I	RGATCY	5 cut(s)	1217, 1760, 1862, 2095, 2242
BstYI	RGATCY	5 cut(s)	1217, 1760, 1862, 2095, 2242
BstZ17I	GTATAC	1 cut(s)	1529
Bsu36I	CCTNAGG	1 cut(s)	12
BsuRI	GGCC	4 cut(s)	756, 918, 1467, 1524
BtrI	CACGTC	1 cut(s)	815
BtsCI	GGATG	5 cut(s)	121, 126, 898, 1403, 2140
BtsIMutI	CAGTG	2 cut(s)	478, 1317
Cac8I	GCNNGC	7 cut(s)	48, 80, 139, 1505, 1605, 1648, 1951
CaiI	CAGNNNCTG	1 cut(s)	2201
CfoI	GCGC	5 cut(s)	250, 1403, 1509, 1935, 2145
Cfr13I	GGNCC	7 cut(s)	63, 192, 243, 755, 782, 917, 1522
CseI	GACGC	1 cut(s)	2207
Csp6I	GTAC	6 cut(s)	609, 1143, 1448, 1748, 1909, 2158
CspCI	CAANNNNNGTGG	4 cut(s)	126, 161, 1746, 1781
CviQI	GTAC	6 cut(s)	609, 1143, 1448, 1748, 1909, 2158
DdeI	CTNAG	7 cut(s)	12, 36, 1310, 1469, 1541, 1642, 2125
DraI	TTTAAA	1 cut(s)	552
DrdI	GACNNNNNNGTC	2 cut(s)	475, 2171
DseDI	GACNNNNNNGTC	2 cut(s)	475, 2171
Eam1104I	CTCTTC	2 cut(s)	698, 1883
EarI	CTCTTC	2 cut(s)	698, 1883
Eco130I	CCWWGG	1 cut(s)	1221
Eco147I	AGGCCT	1 cut(s)	1467
Eco31I	GGTCTC	2 cut(s)	483, 684
Eco32I	GATATC	2 cut(s)	778, 1831
Eco47I	GGWCC	4 cut(s)	63, 192, 243, 782
Eco81I	CCTNAGG	1 cut(s)	12
Eco88I	CYCGRG	1 cut(s)	480
Eco91I	GGTNACC	1 cut(s)	1636
EcoNI	CCTNNNNNAGG	1 cut(s)	1056
EcoO109I	RGGNCCY	2 cut(s)	63, 192
EcoO65I	GGTNACC	1 cut(s)	1636
EcoRI	GAATTC	1 cut(s)	1115
EcoRII	CCWGG	1 cut(s)	920
EcoRV	GATATC	2 cut(s)	778, 1831
EcoT14I	CCWWGG	1 cut(s)	1221
EcoT22I	ATGCAT	2 cut(s)	1196, 1951
ErhI	CCWWGG	1 cut(s)	1221
FalI	AAGNNNNNCTT	4 cut(s)	1144, 1176, 1625, 1657
FaqI	GGGAC	2 cut(s)	256, 1686
FauI	CCCGC	1 cut(s)	87
FbaI	TGATCA	1 cut(s)	220
FblI	GTMKAC	2 cut(s)	1528, 1740
Fnu4HI	GCNGC	5 cut(s)	51, 955, 1501, 1651, 1918
FokI	GGATG	5 cut(s)	128, 133, 885, 1390, 2147
Fsp4HI	GCNGC	5 cut(s)	51, 955, 1501, 1651, 1918
FspBI	CTAG	9 cut(s)	200, 348, 687, 810, 1058, 1077, 1133, 1368, 2070
FspI	TGCGCA	2 cut(s)	1402, 1508
GlaI	GCGC	5 cut(s)	249, 1402, 1508, 1934, 2144
GluI	GCNGC	5 cut(s)	51, 955, 1501, 1651, 1918
GsuI	CTGGAG	1 cut(s)	594
HaeIII	GGCC	4 cut(s)	756, 918, 1467, 1524
HapII	CCGG	2 cut(s)	1734, 2237
HgaI	GACGC	1 cut(s)	2207
HhaI	GCGC	5 cut(s)	250, 1403, 1509, 1935, 2145
Hin6I	GCGC	5 cut(s)	248, 1401, 1507, 1933, 2143
HinP1I	GCGC	5 cut(s)	248, 1401, 1507, 1933, 2143
HindIII	AAGCTT	1 cut(s)	1349
HpaII	CCGG	2 cut(s)	1734, 2237
HphI	GGTGA	7 cut(s)	301, 554, 882, 900, 1391, 1476, 1630
Hpy166II	GTNNAC	4 cut(s)	427, 1484, 1529, 1741
Hpy8I	GTNNAC	4 cut(s)	427, 1484, 1529, 1741
HpyAV	CCTTC	7 cut(s)	533, 1130, 1370, 1513, 1627, 1708, 1851
HpyCH4III	ACNGT	3 cut(s)	1251, 2006, 2077
HpyCH4IV	ACGT	6 cut(s)	423, 611, 655, 814, 1005, 2220
HpyF10VI	GCNNNNNNNGC	3 cut(s)	16, 526, 1506
HpyF3I	CTNAG	7 cut(s)	12, 36, 1310, 1469, 1541, 1642, 2125
HpySE526I	ACGT	6 cut(s)	423, 611, 655, 814, 1005, 2220
HspAI	GCGC	5 cut(s)	248, 1401, 1507, 1933, 2143
Ksp22I	TGATCA	1 cut(s)	220
LguI	GCTCTTC	2 cut(s)	698, 1883
LmnI	GCTCC	7 cut(s)	170, 219, 575, 648, 1183, 1930, 2140
Lsp1109I	GCAGC	4 cut(s)	37, 941, 1662, 1929
LweI	GCATC	6 cut(s)	111, 907, 1021, 1323, 1412, 1936
MaeI	CTAG	9 cut(s)	200, 348, 687, 810, 1058, 1077, 1133, 1368, 2070
MaeII	ACGT	6 cut(s)	423, 611, 655, 814, 1005, 2220
MaeIII	GTNAC	9 cut(s)	253, 448, 466, 906, 1164, 1363, 1492, 1580, 1636
MfeI	CAATTG	2 cut(s)	279, 1532
MflI	RGATCY	5 cut(s)	1217, 1760, 1862, 2095, 2242
MhlI	GDGCHC	4 cut(s)	21, 104, 423, 2017
MlyI	GAGTC	4 cut(s)	261, 392, 492, 1790
MmeI	TCCRAC	2 cut(s)	957, 1860
Mph1103I	ATGCAT	2 cut(s)	1196, 1951
MroXI	GAANNNNTTC	4 cut(s)	379, 1119, 1330, 2252
MseI	TTAA	6 cut(s)	444, 551, 998, 1068, 1458, 1592
MslI	CAYNNNNRTG	3 cut(s)	720, 1514, 1977
MspA1I	CMGCKG	1 cut(s)	1607
MspCI	CTTAAG	1 cut(s)	997
MspI	CCGG	2 cut(s)	1734, 2237
MspR9I	CCNGG	2 cut(s)	922, 2237
MssI	GTTTAAAC	1 cut(s)	552
MunI	CAATTG	2 cut(s)	279, 1532
Mva1269I	GAATGC	1 cut(s)	1509
MvaI	CCWGG	1 cut(s)	922
MvnI	CGCG	3 cut(s)	248, 250, 1935
MwoI	GCNNNNNNNGC	3 cut(s)	16, 526, 1506
NciI	CCSGG	1 cut(s)	2237
NlaIV	GGNNCC	7 cut(s)	18, 64, 244, 919, 1864, 1960, 2244
NmuCI	GTSAC	7 cut(s)	253, 448, 466, 906, 1164, 1492, 1636
NsbI	TGCGCA	2 cut(s)	1402, 1508
NsiI	ATGCAT	2 cut(s)	1196, 1951
NspI	RCATGY	6 cut(s)	108, 1603, 1650, 1953, 1982, 2049
NspV	TTCGAA	1 cut(s)	1236
PaeI	GCATGC	2 cut(s)	1650, 1953
PaeR7I	CTCGAG	1 cut(s)	480
PceI	AGGCCT	1 cut(s)	1467
PciI	ACATGT	2 cut(s)	104, 1978
PciSI	GCTCTTC	2 cut(s)	698, 1883
PcsI	WCGNNNNNNNCGW	1 cut(s)	1593
PctI	GAATGC	1 cut(s)	1509
PdmI	GAANNNNTTC	4 cut(s)	379, 1119, 1330, 2252
PfeI	GAWTC	7 cut(s)	154, 365, 734, 1241, 1287, 1811, 2148
PflMI	CCANNNNNTGG	1 cut(s)	320
PfoI	TCCNGGA	1 cut(s)	2235
PkrI	GCNGC	5 cut(s)	52, 956, 1502, 1652, 1919
PleI	GAGTC	4 cut(s)	260, 392, 491, 1790
PmeI	GTTTAAAC	1 cut(s)	552
PpsI	GAGTC	4 cut(s)	260, 392, 491, 1790
PpuMI	RGGWCCY	2 cut(s)	63, 192
PscI	ACATGT	2 cut(s)	104, 1978
PsiI	TTATAA	1 cut(s)	630
Psp5II	RGGWCCY	2 cut(s)	63, 192
Psp6I	CCWGG	1 cut(s)	920
PspEI	GGTNACC	1 cut(s)	1636
PspGI	CCWGG	1 cut(s)	920
PspN4I	GGNNCC	7 cut(s)	18, 64, 244, 919, 1864, 1960, 2244
PspPI	GGNCC	7 cut(s)	63, 192, 243, 755, 782, 917, 1522
PspPPI	RGGWCCY	2 cut(s)	63, 192
PsrI	GAACNNNNNNTAC	2 cut(s)	2210, 2242
PstI	CTGCAG	2 cut(s)	442, 959
PstNI	CAGNNNCTG	1 cut(s)	2201
PsuI	RGATCY	5 cut(s)	1217, 1760, 1862, 2095, 2242
PvuII	CAGCTG	1 cut(s)	1607
RsaI	GTAC	6 cut(s)	610, 1144, 1449, 1749, 1910, 2159
RsaNI	GTAC	6 cut(s)	609, 1143, 1448, 1748, 1909, 2158
RseI	CAYNNNNRTG	3 cut(s)	720, 1514, 1977
SapI	GCTCTTC	2 cut(s)	698, 1883
SaqAI	TTAA	6 cut(s)	444, 551, 998, 1068, 1458, 1592
SatI	GCNGC	5 cut(s)	51, 955, 1501, 1651, 1918
Sau96I	GGNCC	7 cut(s)	63, 192, 243, 755, 782, 917, 1522
ScaI	AGTACT	1 cut(s)	1144
SchI	GAGTC	4 cut(s)	261, 392, 492, 1790
ScrFI	CCNGG	2 cut(s)	922, 2237
SduI	GDGCHC	4 cut(s)	21, 104, 423, 2017
SfaNI	GCATC	6 cut(s)	111, 907, 1021, 1323, 1412, 1936
SfcI	CTRYAG	2 cut(s)	438, 955
Sfr274I	CTCGAG	1 cut(s)	480
SfuI	TTCGAA	1 cut(s)	1236
SinI	GGWCC	4 cut(s)	63, 192, 243, 782
SlaI	CTCGAG	1 cut(s)	480
SmiMI	CAYNNNNRTG	3 cut(s)	720, 1514, 1977
SmlI	CTYRAG	3 cut(s)	480, 997, 2167
SmoI	CTYRAG	3 cut(s)	480, 997, 2167
SpeI	ACTAGT	1 cut(s)	1367
SphI	GCATGC	2 cut(s)	1650, 1953
SseBI	AGGCCT	1 cut(s)	1467
SsiI	CCGC	9 cut(s)	80, 246, 372, 432, 527, 832, 1337, 1501, 2020
SspMI	CTAG	9 cut(s)	200, 348, 687, 810, 1058, 1077, 1133, 1368, 2070
StuI	AGGCCT	1 cut(s)	1467
StyD4I	CCNGG	2 cut(s)	920, 2235
StyI	CCWWGG	1 cut(s)	1221
TaaI	ACNGT	3 cut(s)	1251, 2006, 2077
TaiI	ACGT	6 cut(s)	426, 614, 658, 817, 1008, 2223
TaqI	TCGA	6 cut(s)	85, 481, 787, 1236, 1418, 2256
TatI	WGTACW	4 cut(s)	1142, 1447, 1747, 2157
TauI	GCSGC	1 cut(s)	1503
TfiI	GAWTC	7 cut(s)	154, 365, 734, 1241, 1287, 1811, 2148
Tru1I	TTAA	6 cut(s)	444, 551, 998, 1068, 1458, 1592
Tru9I	TTAA	6 cut(s)	444, 551, 998, 1068, 1458, 1592
TscAI	CASTG	2 cut(s)	478, 1324
TseFI	GTSAC	7 cut(s)	253, 448, 466, 906, 1164, 1492, 1636
TseI	GCWGC	4 cut(s)	50, 954, 1650, 1917
Tsp45I	GTSAC	7 cut(s)	253, 448, 466, 906, 1164, 1492, 1636
TspDTI	ATGAA	6 cut(s)	257, 402, 474, 1221, 1252, 1556
TspGWI	ACGGA	2 cut(s)	1989, 2027
TspRI	CASTG	2 cut(s)	478, 1324
Van91I	CCANNNNNTGG	1 cut(s)	320
Vha464I	CTTAAG	1 cut(s)	997
VpaK11BI	GGWCC	4 cut(s)	63, 192, 243, 782
XagI	CCTNNNNNAGG	1 cut(s)	1056
XapI	RAATTY	4 cut(s)	203, 1115, 1423, 1657
XbaI	TCTAGA	1 cut(s)	1132
XceI	RCATGY	6 cut(s)	108, 1603, 1650, 1953, 1982, 2049
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1196, 1673
XhoI	CTCGAG	1 cut(s)	480
XmiI	GTMKAC	2 cut(s)	1528, 1740
XmnI	GAANNNNTTC	4 cut(s)	379, 1119, 1330, 2252
XspI	CTAG	9 cut(s)	200, 348, 687, 810, 1058, 1077, 1133, 1368, 2070
ZrmI	AGTACT	1 cut(s)	1144
Zsp2I	ATGCAT	2 cut(s)	1196, 1951

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.