Rh7AG100900

DUF4210

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001019
Physical Location & Seq
Reverse (-)
7790167 .. 7795580
5414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG100900.1

Sequence Viewer

Length: 1620 bp
ATGGGATTGCCTCAAGTGTCTTCTAGCAGTATTGCTGAGGAAGTGGCAGCATCGTTGTGCACTTTTATGCAACCTCCAACCAGAATTGCTGGCGTGAGCAGCTGTGATTTAAGTGGTTTGCATGGCGGAAACATGGGAAATCACATGCAGGTGGATTTGACATGCTCTTCTCCTGGAGAGTTGCAAAGGAAAACCTTGACAGAGATTCCAAATAAGACAGATTTTTCGAATATACGTGTTGGTCGATCAAACATGCATTCCTTGAAGATCAACACCATGGAACAAAATAACTGGTTAAATCTGAAATTAGGTCAGAATATTCAGACATCTGTTCCCAGAATTGTTGGTTTTGAACCAAGAAATTTAAATTCTCCTCTTGATTTGTTTAATGGAAACCGACACTCTTCTAGTGGGGTGGCTGTCACAGATGACACAAATGAGGCCACCGGGTCAGTAGCCAGGAAGCGGTTATCGTCTCCTTTGAATGGAATGCGTCTCCAAGATCAATTTAATGGTGATTCTCTAAACATTGGTGATGGGGTTCATAAGACTTTTGGATGCAAAGATGGCTCTAATATTCCCACATTTCAAGAGCATAAAAAAGCTCACATTGGCAACTCCAATTGTTTCAGTTCTACTACCTGGTCTGCATCTTGTTCCCCAGAGTGGAAGAATTCATTGGATGAGAAGTGTACTACAAACTCCTCCTTTGTAGTTGATGGTCCTTTGCTTGAAACCAAGGAGCCAAAATCACATAATCAGTTCATATCGTCATCTGGACTTGATTACTCCCCAGAAACAATAAAGGTAAGGTCACAATCTGGAGCAATAGCCATAAATTCAAGAAAGGTGGCGGTGCATCCACTTTCTTTATCTCCTCTTGGCCCAAAATTTCCTGAAAGAGTGAAATCTGGAGAAGTATGCAGGGATCATACACCGGGGGCAGATGATTACAGTATGACTTTTAAGGACATGGACCATTCACTTGATGGAACTATCCCAGGCATTTCATCGTCTTGGAAAGAGGAGACTTTTAGGGCTCCAAGTAACTCATTGGAAGATGTTGATATTTTGCAGAAAAAGTTTGACCTCTTTAGTCCTGAGAGTCCCAACGGAATGCAACAGCACTGGAGTCAGGATTTGAAATCCAAGTCTGAAGGTGTTAAATTAGCTCGAAGCCTGACTGGACTACCTGTTAGAAGGTCCTTGGTGGGTTCATTTGAGGAATCCCTCTTGTCTGGGCGATTGGTGTCGGCAAAGGTTAATCAGAGAATTGATGGTTTTCTTGCTGTTCTGAATGTTACTGGAGGGAATTTCTCACCAAAATCACAAAAACTTCCATTTTCCGTGACCAGTGTGGATGGAGAGAACTACTTGCTGTATTATTCATCAATAGATCTTGCTGGGAGAAGGACATCAGAAATCTATGGAGGCCCAAAGATGAAGAGAAGCCTTAGCATGGATGATTCACGAGCAGACAGGAACCGTCTAAGAATACCTATGAAAGGCCGTGTACAACTGGTTCTCAGCAATCCAGAAAAGACACCAATTCATACTTTCATCTGTAACTACGATTTGAGTGACATGCCAGCCGGTAGCAAGGTACTCAGGAAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromosome_seg DUF4210

Protein Analysis

539

Amino Acids

59.04

Weight (kDa)

8.71

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
Acc36I ACCTGC 1 cut(s) 139
AciI CCGC 3 cut(s) 126, 466, 854
AclWI GGATC 1 cut(s) 936
AcsI RAATTY 6 cut(s) 361, 367, 673, 838, 890, 1312
AcuI CTGAAG 1 cut(s) 1176
AfaI GTAC 4 cut(s) 694, 1515, 1605, 1616
AfiI CCNNNNNNNGG 5 cut(s) 465, 485, 666, 1459, 1505
AflIII ACRYGT 1 cut(s) 235
AgsI TTSAA 7 cut(s) 265, 353, 484, 590, 734, 843, 1144
AjnI CCWGG 4 cut(s) 172, 458, 641, 1000
AjuI GAANNNNNNNTTGG 2 cut(s) 662, 694
AluBI AGCT 3 cut(s) 102, 605, 1172
AluI AGCT 3 cut(s) 102, 605, 1172
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 3 cut(s) 480, 500, 1022
Alw44I GTGCAC 1 cut(s) 58
AlwI GGATC 1 cut(s) 936
AoxI GGCC 4 cut(s) 441, 883, 1432, 1507
ApaLI GTGCAC 1 cut(s) 58
ApeKI GCWGC 2 cut(s) 47, 99
ApoI RAATTY 6 cut(s) 361, 367, 673, 838, 890, 1312
ArsI GACNNNNNNTTYG 2 cut(s) 208, 240
AspS9I GGNCC 5 cut(s) 722, 884, 976, 1203, 1433
AsuC2I CCSGG 2 cut(s) 448, 939
AsuHPI GGTGA 3 cut(s) 527, 545, 1311
AsuII TTCGAA 1 cut(s) 227
AvaII GGWCC 3 cut(s) 722, 976, 1203
BaeGI GKGCMC 1 cut(s) 62
BanII GRGCYC 1 cut(s) 1042
BauI CACGAG 1 cut(s) 1470
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 1 cut(s) 62
BbvCI CCTCAGC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 59, 111
BccI CCATC 6 cut(s) 530, 560, 713, 983, 1271, 1355
BceAI ACGGC 1 cut(s) 1494
BciT130I CCWGG 4 cut(s) 174, 460, 643, 1002
BcnI CCSGG 2 cut(s) 448, 939
BcoDI GTCTC 3 cut(s) 480, 500, 1022
BfaI CTAG 2 cut(s) 24, 408
BfuAI ACCTGC 1 cut(s) 139
BglII AGATCT 1 cut(s) 1396
BisI GCNGC 2 cut(s) 48, 100
BlsI GCNGC 2 cut(s) 49, 101
BmcAI AGTACT 1 cut(s) 1616
Bme1390I CCNGG 6 cut(s) 174, 448, 460, 643, 939, 1002
Bme18I GGWCC 3 cut(s) 722, 976, 1203
BmgT120I GGNCC 5 cut(s) 722, 884, 976, 1203, 1433
BmiI GGNNCC 3 cut(s) 744, 1041, 1484
BmrFI CCNGG 6 cut(s) 174, 448, 460, 643, 939, 1002
BmsI GCATC 4 cut(s) 59, 548, 659, 868
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 5 cut(s) 195, 843, 933, 1150, 1326
Bpu10I CCTNAGC 2 cut(s) 36, 1454
Bpu14I TTCGAA 1 cut(s) 227
BpuMI CCSGG 2 cut(s) 448, 939
BsaAI YACGTR 1 cut(s) 236
BsaJI CCNNGG 5 cut(s) 276, 738, 938, 1000, 1206
Bsc4I CCNNNNNNNGG 5 cut(s) 465, 485, 666, 1459, 1505
Bse118I RCCGGY 1 cut(s) 1592
Bse1I ACTGG 6 cut(s) 296, 1133, 1189, 1309, 1353, 1524
BseBI CCWGG 4 cut(s) 174, 460, 643, 1002
BseDI CCNNGG 5 cut(s) 276, 738, 938, 1000, 1206
BseGI GGATG 5 cut(s) 563, 688, 859, 1366, 1468
BseLI CCNNNNNNNGG 5 cut(s) 465, 485, 666, 1459, 1505
BseMII CTCAG 3 cut(s) 27, 1092, 1540
BseNI ACTGG 6 cut(s) 296, 1133, 1189, 1309, 1353, 1524
BseRI GAGGAG 4 cut(s) 363, 694, 867, 1040
BseSI GKGCMC 1 cut(s) 62
BseXI GCAGC 2 cut(s) 59, 111
BseYI CCCAGC 1 cut(s) 1403
BshFI GGCC 4 cut(s) 443, 885, 1434, 1509
BsiHKAI GWGCWC 1 cut(s) 62
BsiSI CCGG 3 cut(s) 447, 938, 1593
BslFI GGGAC 1 cut(s) 1092
BslI CCNNNNNNNGG 5 cut(s) 465, 485, 666, 1459, 1505
BsmAI GTCTC 3 cut(s) 480, 500, 1022
BsmBI CGTCTC 2 cut(s) 480, 500
BsmFI GGGAC 1 cut(s) 1092
BsmI GAATGC 3 cut(s) 256, 495, 1122
BsnI GGCC 4 cut(s) 443, 885, 1434, 1509
Bsp119I TTCGAA 1 cut(s) 227
Bsp1286I GDGCHC 2 cut(s) 62, 1042
Bsp1407I TGTACA 1 cut(s) 1513
Bsp143I GATC 5 cut(s) 245, 267, 502, 928, 1396
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 3 cut(s) 126, 466, 854
BspANI GGCC 4 cut(s) 443, 885, 1434, 1509
BspCNI CTCAG 4 cut(s) 28, 1093, 1539, 1620
BspLI GGNNCC 3 cut(s) 744, 1041, 1484
BspMI ACCTGC 1 cut(s) 139
BspPI GGATC 1 cut(s) 936
BspQI GCTCTTC 1 cut(s) 172
BspT104I TTCGAA 1 cut(s) 227
BsrFI RCCGGY 1 cut(s) 1592
BsrGI TGTACA 1 cut(s) 1513
BsrI ACTGG 6 cut(s) 296, 1133, 1189, 1309, 1353, 1524
BssAI RCCGGY 1 cut(s) 1592
BssECI CCNNGG 5 cut(s) 276, 738, 938, 1000, 1206
BssMI GATC 5 cut(s) 245, 267, 502, 928, 1396
BssSI CACGAG 1 cut(s) 1470
BssT1I CCWWGG 3 cut(s) 276, 738, 1206
Bst2BI CACGAG 1 cut(s) 1470
Bst2UI CCWGG 4 cut(s) 174, 460, 643, 1002
Bst4CI ACNGT 2 cut(s) 956, 1487
Bst6I CTCTTC 3 cut(s) 172, 409, 1439
BstAUI TGTACA 1 cut(s) 1513
BstBAI YACGTR 1 cut(s) 236
BstBI TTCGAA 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 91, 1590
BstDEI CTNAG 6 cut(s) 36, 1101, 1454, 1490, 1526, 1607
BstDSI CCRYGG 1 cut(s) 276
BstENI CCTNNNNNAGG 1 cut(s) 1503
BstF5I GGATG 5 cut(s) 563, 688, 859, 1366, 1468
BstKTI GATC 5 cut(s) 248, 270, 505, 931, 1399
BstMAI GTCTC 3 cut(s) 480, 500, 1022
BstMBI GATC 5 cut(s) 245, 267, 502, 928, 1396
BstMWI GCNNNNNNNGC 2 cut(s) 99, 567
BstNI CCWGG 4 cut(s) 174, 460, 643, 1002
BstNSI RCATGY 4 cut(s) 148, 165, 256, 1588
BstSCI CCNGG 6 cut(s) 172, 446, 458, 641, 937, 1000
BstSLI GKGCMC 1 cut(s) 62
BstV1I GCAGC 2 cut(s) 59, 111
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 1396
BstYI RGATCY 1 cut(s) 1396
BsuRI GGCC 4 cut(s) 443, 885, 1434, 1509
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 5 cut(s) 563, 688, 859, 1366, 1468
BtsIMutI CAGTG 2 cut(s) 1126, 1360
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 2 cut(s) 91, 1590
Cfr10I RCCGGY 1 cut(s) 1592
Cfr13I GGNCC 5 cut(s) 722, 884, 976, 1203, 1433
CseI GACGC 1 cut(s) 482
CsiI ACCWGGT 1 cut(s) 641
Csp6I GTAC 4 cut(s) 693, 1514, 1604, 1615
CspCI CAANNNNNGTGG 2 cut(s) 831, 866
CviAII CATG 9 cut(s) 122, 133, 145, 162, 253, 277, 973, 1459, 1585
CviQI GTAC 4 cut(s) 693, 1514, 1604, 1615
DdeI CTNAG 6 cut(s) 36, 1101, 1454, 1490, 1526, 1607
DpnI GATC 5 cut(s) 247, 269, 504, 930, 1398
DpnII GATC 5 cut(s) 245, 267, 502, 928, 1396
DraI TTTAAA 1 cut(s) 366
Eam1104I CTCTTC 3 cut(s) 172, 409, 1439
EarI CTCTTC 3 cut(s) 172, 409, 1439
EciI GGCGGA 1 cut(s) 141
Eco130I CCWWGG 3 cut(s) 276, 738, 1206
Eco24I GRGCYC 1 cut(s) 1042
Eco47I GGWCC 3 cut(s) 722, 976, 1203
Eco57I CTGAAG 1 cut(s) 1176
EcoNI CCTNNNNNAGG 1 cut(s) 1503
EcoO109I RGGNCCY 1 cut(s) 1203
EcoRI GAATTC 1 cut(s) 673
EcoRII CCWGG 4 cut(s) 172, 458, 641, 1000
EcoT14I CCWWGG 3 cut(s) 276, 738, 1206
EcoT22I ATGCAT 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 1042
ErhI CCWWGG 3 cut(s) 276, 738, 1206
Esp3I CGTCTC 2 cut(s) 480, 500
FaeI CATG 9 cut(s) 125, 136, 148, 165, 256, 280, 976, 1462, 1588
FaqI GGGAC 1 cut(s) 1092
FatI CATG 9 cut(s) 121, 132, 144, 161, 252, 276, 972, 1458, 1584
Fnu4HI GCNGC 2 cut(s) 48, 100
FokI GGATG 5 cut(s) 570, 695, 846, 1373, 1475
FriOI GRGCYC 1 cut(s) 1042
Fsp4HI GCNGC 2 cut(s) 48, 100
FspBI CTAG 2 cut(s) 24, 408
GluI GCNGC 2 cut(s) 48, 100
GsaI CCCAGC 1 cut(s) 1407
GsuI CTGGAG 5 cut(s) 195, 843, 933, 1150, 1326
HaeIII GGCC 4 cut(s) 443, 885, 1434, 1509
HapII CCGG 3 cut(s) 447, 938, 1593
HgaI GACGC 1 cut(s) 482
Hin1II CATG 9 cut(s) 125, 136, 148, 165, 256, 280, 976, 1462, 1588
HinfI GANTC 6 cut(s) 205, 518, 1105, 1132, 1226, 1466
HpaII CCGG 3 cut(s) 447, 938, 1593
HphI GGTGA 3 cut(s) 527, 545, 1311
Hpy166II GTNNAC 3 cut(s) 60, 693, 1514
Hpy188I TCNGA 7 cut(s) 303, 315, 324, 1156, 1269, 1296, 1420
Hpy8I GTNNAC 3 cut(s) 60, 693, 1514
HpyAV CCTTC 3 cut(s) 1151, 1194, 1404
HpyCH4III ACNGT 2 cut(s) 956, 1487
HpyCH4IV ACGT 1 cut(s) 235
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 567
HpyF3I CTNAG 6 cut(s) 36, 1101, 1454, 1490, 1526, 1607
HpySE526I ACGT 1 cut(s) 235
Hsp92II CATG 9 cut(s) 125, 136, 148, 165, 256, 280, 976, 1462, 1588
Kzo9I GATC 5 cut(s) 245, 267, 502, 928, 1396
LguI GCTCTTC 1 cut(s) 172
LmnI GCTCC 3 cut(s) 742, 824, 1045
Lsp1109I GCAGC 2 cut(s) 59, 111
LweI GCATC 4 cut(s) 59, 548, 659, 868
MabI ACCWGGT 1 cut(s) 641
MaeI CTAG 2 cut(s) 24, 408
MaeII ACGT 1 cut(s) 235
MaeIII GTNAC 7 cut(s) 421, 813, 1046, 1300, 1348, 1565, 1580
MalI GATC 5 cut(s) 247, 269, 504, 930, 1398
MboI GATC 5 cut(s) 245, 267, 502, 928, 1396
MboII GAAGA 7 cut(s) 12, 159, 277, 396, 682, 1070, 1456
MfeI CAATTG 1 cut(s) 622
MflI RGATCY 1 cut(s) 1396
MhlI GDGCHC 2 cut(s) 62, 1042
MlyI GAGTC 2 cut(s) 1114, 1141
MmeI TCCRAC 1 cut(s) 101
Mph1103I ATGCAT 1 cut(s) 258
MseI TTAA 8 cut(s) 110, 296, 365, 387, 510, 966, 1164, 1263
MslI CAYNNNNRTG 3 cut(s) 55, 65, 149
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 3 cut(s) 447, 938, 1593
MspR9I CCNGG 6 cut(s) 174, 448, 460, 643, 939, 1002
MunI CAATTG 1 cut(s) 622
Mva1269I GAATGC 3 cut(s) 256, 495, 1122
MvaI CCWGG 4 cut(s) 174, 460, 643, 1002
MwoI GCNNNNNNNGC 2 cut(s) 99, 567
NciI CCSGG 2 cut(s) 448, 939
NcoI CCATGG 1 cut(s) 276
NdeII GATC 5 cut(s) 245, 267, 502, 928, 1396
NlaIII CATG 9 cut(s) 125, 136, 148, 165, 256, 280, 976, 1462, 1588
NlaIV GGNNCC 3 cut(s) 744, 1041, 1484
NmuCI GTSAC 4 cut(s) 421, 813, 1348, 1580
NsiI ATGCAT 1 cut(s) 258
NspI RCATGY 4 cut(s) 148, 165, 256, 1588
NspV TTCGAA 1 cut(s) 227
PaqCI CACCTGC 1 cut(s) 139
PciSI GCTCTTC 1 cut(s) 172
PcsI WCGNNNNNNNCGW 1 cut(s) 241
PctI GAATGC 3 cut(s) 256, 495, 1122
PfeI GAWTC 4 cut(s) 205, 518, 1226, 1466
PfoI TCCNGGA 1 cut(s) 172
PkrI GCNGC 2 cut(s) 49, 101
PleI GAGTC 2 cut(s) 1113, 1140
PpsI GAGTC 2 cut(s) 1113, 1140
Ppu21I YACGTR 1 cut(s) 236
PpuMI RGGWCCY 1 cut(s) 1203
Psp5II RGGWCCY 1 cut(s) 1203
Psp6I CCWGG 4 cut(s) 172, 458, 641, 1000
PspFI CCCAGC 1 cut(s) 1403
PspGI CCWGG 4 cut(s) 172, 458, 641, 1000
PspN4I GGNNCC 3 cut(s) 744, 1041, 1484
PspPI GGNCC 5 cut(s) 722, 884, 976, 1203, 1433
PspPPI RGGWCCY 1 cut(s) 1203
PsrI GAACNNNNNNTAC 2 cut(s) 1506, 1538
PsuI RGATCY 1 cut(s) 1396
PvuII CAGCTG 1 cut(s) 102
RsaI GTAC 4 cut(s) 694, 1515, 1605, 1616
RsaNI GTAC 4 cut(s) 693, 1514, 1604, 1615
RseI CAYNNNNRTG 3 cut(s) 55, 65, 149
SapI GCTCTTC 1 cut(s) 172
SaqAI TTAA 8 cut(s) 110, 296, 365, 387, 510, 966, 1164, 1263
SatI GCNGC 2 cut(s) 48, 100
Sau3AI GATC 5 cut(s) 245, 267, 502, 928, 1396
Sau96I GGNCC 5 cut(s) 722, 884, 976, 1203, 1433
ScaI AGTACT 1 cut(s) 1616
SchI GAGTC 2 cut(s) 1114, 1141
ScrFI CCNGG 6 cut(s) 174, 448, 460, 643, 939, 1002
SduI GDGCHC 2 cut(s) 62, 1042
SexAI ACCWGGT 1 cut(s) 641
SfaNI GCATC 4 cut(s) 59, 548, 659, 868
SfuI TTCGAA 1 cut(s) 227
SinI GGWCC 3 cut(s) 722, 976, 1203
SmiI ATTTAAAT 1 cut(s) 366
SmiMI CAYNNNNRTG 3 cut(s) 55, 65, 149
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
SsiI CCGC 3 cut(s) 126, 466, 854
SspI AATATT 2 cut(s) 319, 577
SspMI CTAG 2 cut(s) 24, 408
StyD4I CCNGG 6 cut(s) 172, 446, 458, 641, 937, 1000
StyI CCWWGG 3 cut(s) 276, 738, 1206
SwaI ATTTAAAT 1 cut(s) 366
TaaI ACNGT 2 cut(s) 956, 1487
TaiI ACGT 1 cut(s) 238
TaqI TCGA 3 cut(s) 227, 244, 1174
TatI WGTACW 3 cut(s) 692, 1513, 1614
TfiI GAWTC 4 cut(s) 205, 518, 1226, 1466
Tru1I TTAA 8 cut(s) 110, 296, 365, 387, 510, 966, 1164, 1263
Tru9I TTAA 8 cut(s) 110, 296, 365, 387, 510, 966, 1164, 1263
TscAI CASTG 2 cut(s) 1133, 1360
TseFI GTSAC 4 cut(s) 421, 813, 1348, 1580
TseI GCWGC 2 cut(s) 47, 99
Tsp45I GTSAC 4 cut(s) 421, 813, 1348, 1580
TspGWI ACGGA 2 cut(s) 1128, 1336
TspRI CASTG 2 cut(s) 1133, 1360
VneI GTGCAC 1 cut(s) 58
VpaK11BI GGWCC 3 cut(s) 722, 976, 1203
XagI CCTNNNNNAGG 1 cut(s) 1503
XapI RAATTY 6 cut(s) 361, 367, 673, 838, 890, 1312
XceI RCATGY 4 cut(s) 148, 165, 256, 1588
XcmI CCANNNNNNNNNTGG 1 cut(s) 986
XspI CTAG 2 cut(s) 24, 408
ZrmI AGTACT 1 cut(s) 1616
Zsp2I ATGCAT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.