FvH4_5g14530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007742
Physical Location & Seq
Reverse (-)
8231093 .. 8232741
1649 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14530.t1

Sequence Viewer

Length: 567 bp
ATGGATGGCGGCCAATTCGAATTCGATCCAGACGGCTCAGAATTCAAACGCTGGCAGAAAATCTCCAACACCAAATCCAGAGAATGGTCAATGGTAGCCATCAGAGACTCCACCTCCTCCGTTTTCCCTCCAATAAACCACGAAAACCTCCACCTCCCAACCTCCCCATCACTCTCGTCTTCTTCCTCCTCCTCTCTCTCCTCGTTTTCTCCGTCCGATTCCGACGAATCGGGCCCGGTTTCGCCGACGAAGGTCAATACCCAGGTCGGGAAATGCAGTGAATTGAGAAGCTGGGTGGGTCTTGGAGTTGGGTTGCTGCGTTCTAGAATTTTGGGGATTGGAGGGTTGGTTAAACGTTGGGGCTTTTCTTCTGGGTATGTTGTGGCGGTGGTGTTTATGGTGTGGTCGTTTTACTTGCGAGTGAAGCAGAGGCGGTATCGGAGGCGTTGCGAGTTGGTGATCAAGGAGAAAGATGAGAAGATTAGTAAGCTGCTGAACCAAATCGCGCAATTGAACGAAGTGCTGGTAGCTCGGCATAGAGTTCTGGCTTCCAAACTGGCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

21.05

Weight (kDa)

9.95

Isoelectric Point (pI)

60.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 84
AccII CGCG 1 cut(s) 506
AciI CCGC 3 cut(s) 9, 386, 433
AclI AACGTT 1 cut(s) 355
AclWI GGATC 1 cut(s) 20
AcoI YGGCCR 1 cut(s) 10
AcsI RAATTY 3 cut(s) 20, 41, 327
AfiI CCNNNNNNNGG 2 cut(s) 84, 267
AgsI TTSAA 2 cut(s) 46, 514
AjnI CCWGG 1 cut(s) 261
AluBI AGCT 3 cut(s) 291, 490, 530
AluI AGCT 3 cut(s) 291, 490, 530
Alw26I GTCTC 1 cut(s) 99
AlwI GGATC 1 cut(s) 20
AoxI GGCC 2 cut(s) 10, 232
ApaI GGGCCC 1 cut(s) 236
ApeKI GCWGC 2 cut(s) 316, 490
ApoI RAATTY 3 cut(s) 20, 41, 327
AspLEI GCGC 1 cut(s) 508
AspS9I GGNCC 2 cut(s) 232, 233
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 1 cut(s) 469
AsuII TTCGAA 1 cut(s) 18
BaeGI GKGCMC 1 cut(s) 236
BanII GRGCYC 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 303, 477
BccI CCATC 2 cut(s) 107, 175
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 1 cut(s) 263
BclI TGATCA 1 cut(s) 459
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 99
BfaI CTAG 1 cut(s) 324
BisI GCNGC 3 cut(s) 10, 317, 491
BlsI GCNGC 3 cut(s) 11, 318, 492
Bme1390I CCNGG 2 cut(s) 236, 263
BmgT120I GGNCC 2 cut(s) 232, 233
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 2 cut(s) 236, 263
BoxI GACNNNNGTC 1 cut(s) 251
BpiI GAAGAC 1 cut(s) 171
Bpu14I TTCGAA 1 cut(s) 18
BpuMI CCSGG 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 261
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 333, 363
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 267
Bse1I ACTGG 1 cut(s) 561
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 84, 267
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 561
BseRI GAGGAG 4 cut(s) 106, 178, 181, 190
BseSI GKGCMC 1 cut(s) 236
BseXI GCAGC 2 cut(s) 303, 477
BseYI CCCAGC 1 cut(s) 291
Bsh1236I CGCG 1 cut(s) 506
BshFI GGCC 2 cut(s) 12, 234
BsiSI CCGG 1 cut(s) 236
BslI CCNNNNNNNGG 2 cut(s) 84, 267
BsmAI GTCTC 1 cut(s) 99
BsnI GGCC 2 cut(s) 12, 234
Bsp119I TTCGAA 1 cut(s) 18
Bsp120I GGGCCC 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 2 cut(s) 25, 459
BspACI CCGC 3 cut(s) 9, 386, 433
BspANI GGCC 2 cut(s) 12, 234
BspCNI CTCAG 1 cut(s) 50
BspFNI CGCG 1 cut(s) 506
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 1 cut(s) 20
BspT104I TTCGAA 1 cut(s) 18
BsrI ACTGG 1 cut(s) 561
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 2 cut(s) 25, 459
Bst2UI CCWGG 1 cut(s) 263
BstBI TTCGAA 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 53
BstDEI CTNAG 1 cut(s) 37
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 506
BstHHI GCGC 1 cut(s) 508
BstKTI GATC 2 cut(s) 28, 462
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 2 cut(s) 25, 459
BstMWI GCNNNNNNNGC 1 cut(s) 424
BstNI CCWGG 1 cut(s) 263
BstPAI GACNNNNGTC 1 cut(s) 251
BstSCI CCNGG 2 cut(s) 234, 261
BstSLI GKGCMC 1 cut(s) 236
BstUI CGCG 1 cut(s) 506
BstV1I GCAGC 2 cut(s) 303, 477
BstV2I GAAGAC 1 cut(s) 171
BsuRI GGCC 2 cut(s) 12, 234
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 283
Cac8I GCNNGC 1 cut(s) 53
CfoI GCGC 1 cut(s) 508
Cfr13I GGNCC 2 cut(s) 232, 233
DdeI CTNAG 1 cut(s) 37
DpnI GATC 2 cut(s) 27, 461
DpnII GATC 2 cut(s) 25, 459
EaeI YGGCCR 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 236
EcoRI GAATTC 2 cut(s) 20, 41
EcoRII CCWGG 1 cut(s) 261
EcoT38I GRGCYC 1 cut(s) 236
FaiI YATR 3 cut(s) 378, 398, 537
FbaI TGATCA 1 cut(s) 459
Fnu4HI GCNGC 3 cut(s) 10, 317, 491
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 3 cut(s) 10, 317, 491
FspBI CTAG 1 cut(s) 324
GlaI GCGC 1 cut(s) 507
GluI GCNGC 3 cut(s) 10, 317, 491
GsaI CCCAGC 1 cut(s) 295
HaeIII GGCC 2 cut(s) 12, 234
HapII CCGG 1 cut(s) 236
HhaI GCGC 1 cut(s) 508
Hin6I GCGC 1 cut(s) 506
HinP1I GCGC 1 cut(s) 506
HinfI GANTC 3 cut(s) 107, 218, 227
HpaII CCGG 1 cut(s) 236
HphI GGTGA 1 cut(s) 469
Hpy188I TCNGA 5 cut(s) 40, 104, 217, 223, 441
Hpy188III TCNNGA 4 cut(s) 29, 78, 268, 324
Hpy99I CGWCG 2 cut(s) 227, 250
HpyAV CCTTC 1 cut(s) 244
HpyCH4IV ACGT 1 cut(s) 355
HpyCH4V TGCA 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 1 cut(s) 424
HpyF3I CTNAG 1 cut(s) 37
HpySE526I ACGT 1 cut(s) 355
HspAI GCGC 1 cut(s) 506
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 2 cut(s) 25, 459
Lsp1109I GCAGC 2 cut(s) 303, 477
MaeI CTAG 1 cut(s) 324
MaeII ACGT 1 cut(s) 355
MalI GATC 2 cut(s) 27, 461
MboI GATC 2 cut(s) 25, 459
MboII GAAGA 4 cut(s) 171, 174, 360, 490
MfeI CAATTG 1 cut(s) 509
MhlI GDGCHC 1 cut(s) 236
MluCI AATT 7 cut(s) 14, 20, 41, 281, 327, 509, 562
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 2 cut(s) 90, 246
MseI TTAA 1 cut(s) 351
MspI CCGG 1 cut(s) 236
MspR9I CCNGG 2 cut(s) 236, 263
MunI CAATTG 1 cut(s) 509
MvaI CCWGG 1 cut(s) 263
MvnI CGCG 1 cut(s) 506
MwoI GCNNNNNNNGC 1 cut(s) 424
NciI CCSGG 1 cut(s) 236
NdeII GATC 2 cut(s) 25, 459
NlaIV GGNNCC 1 cut(s) 234
NmeAIII GCCGAG 1 cut(s) 511
NspV TTCGAA 1 cut(s) 18
PcsI WCGNNNNNNNCGW 1 cut(s) 209
PfeI GAWTC 2 cut(s) 218, 227
PflMI CCANNNNNTGG 1 cut(s) 84
PkrI GCNGC 3 cut(s) 11, 318, 492
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PshAI GACNNNNGTC 1 cut(s) 251
Psp1406I AACGTT 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 261
PspFI CCCAGC 1 cut(s) 291
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 1 cut(s) 234
PspOMI GGGCCC 1 cut(s) 232
PspPI GGNCC 2 cut(s) 232, 233
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 3 cut(s) 10, 317, 491
Sau3AI GATC 2 cut(s) 25, 459
Sau96I GGNCC 2 cut(s) 232, 233
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 2 cut(s) 236, 263
SduI GDGCHC 1 cut(s) 236
SfuI TTCGAA 1 cut(s) 18
Sse9I AATT 7 cut(s) 14, 20, 41, 281, 327, 509, 562
SsiI CCGC 3 cut(s) 9, 386, 433
SspMI CTAG 1 cut(s) 324
StyD4I CCNGG 2 cut(s) 234, 261
TaiI ACGT 1 cut(s) 358
TaqI TCGA 2 cut(s) 18, 24
TasI AATT 7 cut(s) 14, 20, 41, 281, 327, 509, 562
TauI GCSGC 1 cut(s) 12
TfiI GAWTC 2 cut(s) 218, 227
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 283
TseI GCWGC 2 cut(s) 316, 490
TspGWI ACGGA 2 cut(s) 109, 201
TspRI CASTG 1 cut(s) 283
Van91I CCANNNNNTGG 1 cut(s) 84
XapI RAATTY 3 cut(s) 20, 41, 327
XbaI TCTAGA 1 cut(s) 323
XspI CTAG 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.