FvH4_5g16840

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005794
Physical Location & Seq
Forward (+)
9623643 .. 9627004
3362 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16840.t2

Sequence Viewer

Length: 678 bp
ATGCTCAGAAGCATTTGCATGCCAATGTGCTTTCGGCTGGATTACCACTTGAATGTGAAAGCTGCCAAGAAAGCCATTCTTGATGGTTTTCGGAAAACTGATGAGGCTATTCTTCAAGAAAGTGCTGCAGTGGGATGGCAAGATGGTGCTACAGCTGTATGTGTTTGGATATTAGGAAAAACAGTATTTGTTGCTAATATTGGGGATGCAAAAGCTGTGGTAGCAAGATCATCTCCTGTTGATGGATTGCAAAATGGTTCTGATGAAGCGCGTCTTTTGAAGGCCATTGTTTTAACAAGAGAACACAAAGCCATCTACGCACAGGAGCGTGCTCGTATTCAGAAGGCTGGTGGTAGTGTGGGTTCAAATGGACGACTACAGGGTCGCCTTGAAGTTTCTAGGGCCTTTGGAGACCGCCAGTTCAAAAAGTTTGGTGTTGTTGCTACCCCAGATATCCATTCATTTGACCTTACAGAAAGAGAGCACTTCATAATTCTTGGTTGCGACGGCCTCTGGGGAGTAATTGGACCAAGTGATGCTGTTGATTTTGTTCAGAAACAGCTGAAGGAGGGATTACCTGTTATGGCTATTAGCCGCTCTCTAGTAAGAGAAGCTGTGCGTGAGCGGCGCTGTAAAGATAACTGTACGGCAATCATAATCCTATTAAGGCACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

226

Amino Acids

24.79

Weight (kDa)

9.43

Isoelectric Point (pI)

49.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 58 - 278 1.5e-47 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 381
AccBSI CCGCTC 2 cut(s) 597, 625
AccII CGCG 1 cut(s) 271
AciI CCGC 3 cut(s) 415, 595, 625
AcuI CTGAAG 1 cut(s) 584
AfaI GTAC 1 cut(s) 646
AfiI CCNNNNNNNGG 1 cut(s) 242
AgsI TTSAA 6 cut(s) 52, 116, 280, 366, 392, 424
AluBI AGCT 5 cut(s) 62, 155, 215, 562, 614
AluI AGCT 5 cut(s) 62, 155, 215, 562, 614
Alw21I GWGCWC 2 cut(s) 334, 486
Alw26I GTCTC 1 cut(s) 405
AoxI GGCC 3 cut(s) 282, 402, 508
ApeKI GCWGC 2 cut(s) 62, 125
AspLEI GCGC 2 cut(s) 271, 630
AspS9I GGNCC 2 cut(s) 402, 527
AvaII GGWCC 1 cut(s) 527
Bbv12I GWGCWC 2 cut(s) 334, 486
BbvI GCAGC 2 cut(s) 49, 112
BccI CCATC 5 cut(s) 77, 129, 137, 236, 320
BceAI ACGGC 2 cut(s) 523, 663
BcoDI GTCTC 1 cut(s) 405
BfaI CTAG 2 cut(s) 399, 602
BfmI CTRYAG 3 cut(s) 126, 150, 377
BfoI RGCGCY 1 cut(s) 631
BisI GCNGC 4 cut(s) 63, 126, 595, 626
BlsI GCNGC 4 cut(s) 64, 127, 596, 627
Bme18I GGWCC 1 cut(s) 527
BmgT120I GGNCC 2 cut(s) 402, 527
BmsI GCATC 2 cut(s) 196, 526
BsaI GGTCTC 1 cut(s) 405
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 418
BseGI GGATG 2 cut(s) 140, 211
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMII CTCAG 1 cut(s) 19
BseNI ACTGG 1 cut(s) 418
BseXI GCAGC 2 cut(s) 49, 112
Bsh1236I CGCG 1 cut(s) 271
BshFI GGCC 3 cut(s) 284, 404, 510
BsiHKAI GWGCWC 2 cut(s) 334, 486
BslI CCNNNNNNNGG 1 cut(s) 242
BsmAI GTCTC 1 cut(s) 405
BsnI GGCC 3 cut(s) 284, 404, 510
Bso31I GGTCTC 1 cut(s) 405
Bsp1286I GDGCHC 2 cut(s) 334, 486
Bsp143I GATC 1 cut(s) 227
BspACI CCGC 3 cut(s) 415, 595, 625
BspANI GGCC 3 cut(s) 284, 404, 510
BspCNI CTCAG 1 cut(s) 18
BspFNI CGCG 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 130
BspTNI GGTCTC 1 cut(s) 405
BsrBI CCGCTC 2 cut(s) 597, 625
BsrI ACTGG 1 cut(s) 418
BssMI GATC 1 cut(s) 227
Bst4CI ACNGT 2 cut(s) 184, 644
BstC8I GCNNGC 2 cut(s) 20, 330
BstDEI CTNAG 1 cut(s) 5
BstF5I GGATG 2 cut(s) 140, 211
BstFNI CGCG 1 cut(s) 271
BstH2I RGCGCY 1 cut(s) 631
BstHHI GCGC 2 cut(s) 271, 630
BstKTI GATC 1 cut(s) 230
BstMAI GTCTC 1 cut(s) 405
BstMBI GATC 1 cut(s) 227
BstMWI GCNNNNNNNGC 4 cut(s) 71, 221, 317, 625
BstNSI RCATGY 1 cut(s) 22
BstSFI CTRYAG 3 cut(s) 126, 150, 377
BstUI CGCG 1 cut(s) 271
BstV1I GCAGC 2 cut(s) 49, 112
BsuRI GGCC 3 cut(s) 284, 404, 510
BtsCI GGATG 2 cut(s) 140, 211
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 2 cut(s) 20, 330
CfoI GCGC 2 cut(s) 271, 630
Cfr13I GGNCC 2 cut(s) 402, 527
CseI GACGC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 645
CspCI CAANNNNNGTGG 2 cut(s) 198, 233
CviAII CATG 1 cut(s) 19
CviQI GTAC 1 cut(s) 645
DdeI CTNAG 1 cut(s) 5
DpnI GATC 1 cut(s) 229
DpnII GATC 1 cut(s) 227
DrdI GACNNNNNNGTC 1 cut(s) 381
DseDI GACNNNNNNGTC 1 cut(s) 381
Eco31I GGTCTC 1 cut(s) 405
Eco32I GATATC 1 cut(s) 454
Eco47I GGWCC 1 cut(s) 527
Eco57I CTGAAG 1 cut(s) 584
EcoO109I RGGNCCY 1 cut(s) 402
EcoRV GATATC 1 cut(s) 454
FaeI CATG 1 cut(s) 22
FaiI YATR 5 cut(s) 20, 160, 491, 584, 656
FalI AAGNNNNNCTT 4 cut(s) 63, 95, 258, 290
FatI CATG 1 cut(s) 18
Fnu4HI GCNGC 4 cut(s) 63, 126, 595, 626
FokI GGATG 2 cut(s) 147, 218
Fsp4HI GCNGC 4 cut(s) 63, 126, 595, 626
FspBI CTAG 2 cut(s) 399, 602
GlaI GCGC 2 cut(s) 270, 629
GluI GCNGC 4 cut(s) 63, 126, 595, 626
HaeII RGCGCY 1 cut(s) 631
HaeIII GGCC 3 cut(s) 284, 404, 510
HgaI GACGC 1 cut(s) 260
HhaI GCGC 2 cut(s) 271, 630
Hin1II CATG 1 cut(s) 22
Hin6I GCGC 2 cut(s) 269, 628
HinP1I GCGC 2 cut(s) 269, 628
Hpy188I TCNGA 5 cut(s) 8, 93, 262, 342, 555
Hpy188III TCNNGA 2 cut(s) 80, 116
Hpy99I CGWCG 1 cut(s) 509
HpyAV CCTTC 3 cut(s) 274, 337, 559
HpyCH4III ACNGT 2 cut(s) 184, 644
HpyCH4V TGCA 4 cut(s) 18, 128, 209, 250
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 221, 317, 625
HpyF3I CTNAG 1 cut(s) 5
Hsp92II CATG 1 cut(s) 22
HspAI GCGC 2 cut(s) 269, 628
Kzo9I GATC 1 cut(s) 227
LmnI GCTCC 1 cut(s) 325
LpnPI CCDG 9 cut(s) 23, 249, 308, 333, 365, 431, 462, 499, 591
Lsp1109I GCAGC 2 cut(s) 49, 112
LweI GCATC 2 cut(s) 196, 526
MaeI CTAG 2 cut(s) 399, 602
MalI GATC 1 cut(s) 229
MbiI CCGCTC 2 cut(s) 597, 625
MboI GATC 1 cut(s) 227
MboII GAAGA 1 cut(s) 104
MhlI GDGCHC 2 cut(s) 334, 486
MluCI AATT 2 cut(s) 492, 522
MnlI CCTC 3 cut(s) 97, 521, 562
MseI TTAA 2 cut(s) 293, 665
MslI CAYNNNNRTG 3 cut(s) 17, 23, 51
MspA1I CMGCKG 2 cut(s) 155, 562
MvnI CGCG 1 cut(s) 271
MwoI GCNNNNNNNGC 4 cut(s) 71, 221, 317, 625
NdeII GATC 1 cut(s) 227
NlaIII CATG 1 cut(s) 22
NspI RCATGY 1 cut(s) 22
PaeI GCATGC 1 cut(s) 22
PkrI GCNGC 4 cut(s) 64, 127, 596, 627
PspPI GGNCC 2 cut(s) 402, 527
PsrI GAACNNNNNNTAC 2 cut(s) 346, 378
PstI CTGCAG 1 cut(s) 130
PvuII CAGCTG 2 cut(s) 155, 562
RsaI GTAC 1 cut(s) 646
RsaNI GTAC 1 cut(s) 645
RseI CAYNNNNRTG 3 cut(s) 17, 23, 51
SaqAI TTAA 2 cut(s) 293, 665
SatI GCNGC 4 cut(s) 63, 126, 595, 626
Sau3AI GATC 1 cut(s) 227
Sau96I GGNCC 2 cut(s) 402, 527
SduI GDGCHC 2 cut(s) 334, 486
SetI ASST 7 cut(s) 64, 157, 217, 471, 564, 580, 616
SfaNI GCATC 2 cut(s) 196, 526
SfcI CTRYAG 3 cut(s) 126, 150, 377
SinI GGWCC 1 cut(s) 527
SmiMI CAYNNNNRTG 3 cut(s) 17, 23, 51
SphI GCATGC 1 cut(s) 22
Sse9I AATT 2 cut(s) 492, 522
SsiI CCGC 3 cut(s) 415, 595, 625
SspI AATATT 1 cut(s) 199
SspMI CTAG 2 cut(s) 399, 602
TaaI ACNGT 2 cut(s) 184, 644
TasI AATT 2 cut(s) 492, 522
TauI GCSGC 2 cut(s) 597, 628
Tru1I TTAA 2 cut(s) 293, 665
Tru9I TTAA 2 cut(s) 293, 665
TscAI CASTG 1 cut(s) 135
TseI GCWGC 2 cut(s) 62, 125
TspDTI ATGAA 3 cut(s) 279, 450, 478
TspRI CASTG 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 527
XceI RCATGY 1 cut(s) 22
XspI CTAG 2 cut(s) 399, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.